AlfalfaGEDB | MsG0680035349.01

M. sativa cultivar ZhongmuNo.1 / MsG0680035349.01

KEGG: -
GO: GO:0006869
PFAM: PF14368

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035349.01.T01	XP_003620406.1	97.826	92	2	1	92	1	92	5.71E-57	180
MsG0680035349.01.T03	XP_003620406.1	100	29	0	1	29	64	92	1.09E-11	63.2
MsG0680035349.01.T02	XP_003620406.1	100	70	0	1	70	23	92	5.86E-42	141

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035349.01.T01	Q43681	55	80	35	1	14	92	20	99	1.42E-25	94
MsG0680035349.01.T02	P82353	61.765	68	26	0	3	70	1	68	1.09E-25	92.4

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035349.01.T01	B7FM99	97.826	92	2	1	92	1	92	2.73e-57	180
MsG0680035349.01.T02	B7FM99	100.000	70	0	1	70	23	92	2.80e-42	141
MsG0680035349.01.T03	B7FM99	100.000	29	0	1	29	64	92	5.21e-12	63.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048633.01	MsG0680035349.01	0.803002	4.290742e-49	2.132122e-46
MsG0080048652.01	MsG0680035349.01	0.816746	4.718317e-52	3.371553e-49
MsG0180000111.01	MsG0680035349.01	0.808161	3.547964e-50	2.014014e-47
MsG0180003360.01	MsG0680035349.01	0.855264	7.087251e-62	1.626745e-58
MsG0180003427.01	MsG0680035349.01	0.822096	2.844024e-53	2.357191e-50
MsG0180004688.01	MsG0680035349.01	0.802370	5.794568e-49	2.833677e-46
MsG0380014130.01	MsG0680035349.01	0.816133	6.469956e-52	4.546417e-49
MsG0380014132.01	MsG0680035349.01	0.845480	3.956055e-59	6.601816e-56
MsG0380014594.01	MsG0680035349.01	0.835374	1.742015e-56	2.126190e-53
MsG0380014881.01	MsG0680035349.01	0.820231	7.650236e-53	6.018544e-50
MsG0680035349.01	MsG0780041271.01	0.856357	3.397379e-62	8.087625e-59
MsG0680035349.01	MsG0780041820.01	0.802002	6.897396e-49	3.341314e-46
MsG0680035349.01	MsG0880043855.01	0.805559	1.259252e-49	6.682619e-47
MsG0680035349.01	MsG0880043948.01	0.805532	1.275660e-49	6.765055e-47
MsG0680035349.01	MsG0880043951.01	0.800013	1.758944e-48	8.102064e-46
MsG0680035349.01	MsG0880044984.01	0.813156	2.952216e-51	1.913013e-48
MsG0480019699.01	MsG0680035349.01	0.813115	3.012942e-51	1.950220e-48
MsG0480022873.01	MsG0680035349.01	0.840660	7.608603e-58	1.091211e-54
MsG0480023195.01	MsG0680035349.01	0.808651	2.789829e-50	1.603956e-47
MsG0480023532.01	MsG0680035349.01	0.819349	1.216787e-52	9.339499e-50
MsG0380017819.01	MsG0680035349.01	0.858242	9.424554e-63	2.389370e-59
MsG0380017989.01	MsG0680035349.01	0.806869	6.669637e-50	3.660468e-47
MsG0280008407.01	MsG0680035349.01	0.819894	9.138798e-53	7.122801e-50
MsG0280010303.01	MsG0680035349.01	0.849487	3.132793e-60	5.948199e-57

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035349.01.T01	MTR_6g082480	97.826	92	2	0	1	92	1	92	6.92e-61	180
MsG0680035349.01.T01	MTR_1g109650	58.696	92	38	0	1	92	1	92	9.01e-38	122
MsG0680035349.01.T01	MTR_7g007440	50.633	79	39	0	14	92	20	98	5.41e-29	100
MsG0680035349.01.T01	MTR_7g082640	48.864	88	42	1	8	92	7	94	4.09e-28	97.8
MsG0680035349.01.T01	MTR_7g082590	38.372	86	50	1	8	90	7	92	7.42e-19	74.3
MsG0680035349.01.T01	MTR_4g077720	32.609	92	36	1	1	92	1	66	1.96e-12	57.4
MsG0680035349.01.T03	MTR_6g082480	100.000	29	0	0	1	29	64	92	1.32e-15	63.2
MsG0680035349.01.T02	MTR_6g082480	100.000	70	0	0	1	70	23	92	7.09e-46	141
MsG0680035349.01.T02	MTR_1g109650	65.714	70	24	0	1	70	23	92	7.44e-32	106
MsG0680035349.01.T02	MTR_7g007440	56.522	69	30	0	2	70	30	98	3.21e-28	97.4
MsG0680035349.01.T02	MTR_7g082640	57.971	69	29	0	2	70	26	94	6.80e-26	91.3
MsG0680035349.01.T02	MTR_7g082590	43.284	67	38	0	2	68	26	92	2.32e-16	67.0
MsG0680035349.01.T02	MTR_4g077720	55.000	40	18	0	31	70	27	66	2.64e-12	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035349.01.T01	AT3G18280	65.000	80	28	0	13	92	17	96	1.41e-34	114
MsG0680035349.01.T01	AT3G18280	65.000	80	28	0	13	92	41	120	2.78e-34	114
MsG0680035349.01.T01	AT1G48750	60.811	74	29	0	19	92	21	94	8.22e-30	102
MsG0680035349.01.T01	AT1G73780	48.837	86	42	1	9	92	13	98	2.93e-26	93.2
MsG0680035349.01.T01	AT5G38170	50.000	68	34	0	25	92	36	103	5.63e-22	82.4
MsG0680035349.01.T01	AT5G38160	48.529	68	35	0	25	92	36	103	9.70e-20	77.0
MsG0680035349.01.T01	AT1G66850	43.662	71	40	0	22	92	32	102	3.76e-18	72.8
MsG0680035349.01.T01	AT5G38195	39.189	74	45	0	19	92	22	95	1.80e-15	65.9
MsG0680035349.01.T01	AT1G43665	37.234	94	51	2	4	91	5	96	3.43e-15	65.9
MsG0680035349.01.T01	AT1G43666	38.028	71	42	1	22	92	27	95	1.43e-14	63.5
MsG0680035349.01.T01	AT1G07747	31.522	92	61	1	1	92	3	92	2.35e-14	63.2
MsG0680035349.01.T01	AT5G38180	32.632	95	61	1	1	92	1	95	2.59e-13	60.5
MsG0680035349.01.T01	AT2G14846	40.323	62	37	0	31	92	38	99	6.22e-13	59.3
MsG0680035349.01.T01	AT1G43667	31.000	100	59	3	1	92	1	98	1.22e-12	58.5
MsG0680035349.01.T01	AT3G57310	38.806	67	41	0	26	92	37	103	3.55e-12	57.8
MsG0680035349.01.T01	AT4G12825	38.806	67	39	1	26	92	31	95	8.24e-12	56.6
MsG0680035349.01.T01	AT5G38197	31.959	97	60	2	1	92	1	96	1.12e-11	56.2
MsG0680035349.01.T02	AT3G18280	72.464	69	19	0	2	70	28	96	4.33e-33	109
MsG0680035349.01.T02	AT3G18280	72.464	69	19	0	2	70	52	120	7.94e-33	109
MsG0680035349.01.T02	AT1G48750	62.319	69	26	0	2	70	26	94	9.93e-29	98.6
MsG0680035349.01.T02	AT1G73780	53.030	66	31	0	5	70	33	98	1.68e-23	85.5
MsG0680035349.01.T02	AT5G38170	50.000	68	34	0	3	70	36	103	9.05e-22	81.3
MsG0680035349.01.T02	AT5G38160	48.529	68	35	0	3	70	36	103	9.84e-20	75.9
MsG0680035349.01.T02	AT1G66850	44.286	70	39	0	1	70	33	102	2.93e-18	72.0
MsG0680035349.01.T02	AT5G38195	43.284	67	38	0	4	70	29	95	2.28e-15	64.7
MsG0680035349.01.T02	AT1G43665	44.776	67	35	1	3	69	32	96	7.16e-15	64.3
MsG0680035349.01.T02	AT1G43666	40.299	67	38	1	4	70	31	95	1.81e-14	62.4
MsG0680035349.01.T02	AT2G14846	40.323	62	37	0	9	70	38	99	3.53e-13	59.3
MsG0680035349.01.T02	AT1G07747	37.879	66	39	1	5	70	29	92	1.11e-12	57.8
MsG0680035349.01.T02	AT3G57310	38.806	67	41	0	4	70	37	103	1.46e-12	57.8
MsG0680035349.01.T02	AT5G38180	38.806	67	41	0	4	70	29	95	5.45e-12	56.2
MsG0680035349.01.T02	AT4G12825	38.806	67	39	1	4	70	31	95	5.75e-12	55.8
MsG0680035349.01.T02	AT1G43667	32.353	68	44	1	3	70	33	98	1.06e-11	55.5

Find 18 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTCTTTGCTCTTTGATTT+TGG	0.172074	6:-103014396	None:intergenic
GTTAACAAACTTCCTAAGAT+TGG	0.334175	6:-103014444	None:intergenic
ACATGAAGAATCCCAATCTT+AGG	0.336088	6:+103014432	MsG0680035349.01.T01:CDS
GATTGGGATTCTTCATGTAT+TGG	0.352202	6:-103014427	None:intergenic
TTGGAGCAGCATAGGTTAGA+TGG	0.365920	6:-103014377	None:intergenic
TTAGCAACTTTCCTTGCATT+AGG	0.379178	6:-103014470	None:intergenic
TTAACAAACTTCCTAAGATT+GGG	0.387314	6:-103014443	None:intergenic
AACATTTAACACCTTGGAAA+AGG	0.413827	6:-103014506	None:intergenic
ACACATGAGCTGAGCTGCAC+TGG	0.449963	6:-103014329	None:intergenic
GGTTAATGTTCAAGTGTCAA+TGG	0.483776	6:+103014292	MsG0680035349.01.T01:CDS
AGTTTGAACATTTAACACCT+TGG	0.520743	6:-103014512	None:intergenic
CATTAACCAAAAGCAGCAAA+AGG	0.535941	6:-103014277	None:intergenic
GATTCTTCATGTATTGGCAA+AGG	0.553986	6:-103014421	None:intergenic
AGGAAAGTTGCTAACACTTG+TGG	0.574369	6:+103014479	MsG0680035349.01.T01:CDS
TTCATGTATTGGCAAAGGCA+AGG	0.575594	6:-103014416	None:intergenic
AACCAAAAGCAGCAAAAGGA+AGG	0.589777	6:-103014273	None:intergenic
TTGTTAACACTCCTAATGCA+AGG	0.595933	6:+103014459	MsG0680035349.01.T01:CDS
GAGCAGCATAGGTTAGATGG+AGG	0.635595	6:-103014374	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTAACAAACTTCCTAAGATT+GGG	-	Chr6:103014446-103014465	None:intergenic	25.0%
	GTTAACAAACTTCCTAAGAT+TGG	-	Chr6:103014447-103014466	None:intergenic	30.0%
	ACATGAAGAATCCCAATCTT+AGG	+	Chr6:103014432-103014451	MsG0680035349.01.T01:CDS	35.0%
	CATTAACCAAAAGCAGCAAA+AGG	-	Chr6:103014280-103014299	None:intergenic	35.0%
	GATTGGGATTCTTCATGTAT+TGG	-	Chr6:103014430-103014449	None:intergenic	35.0%
	GGTTAATGTTCAAGTGTCAA+TGG	+	Chr6:103014292-103014311	MsG0680035349.01.T01:CDS	35.0%
	TTAGCAACTTTCCTTGCATT+AGG	-	Chr6:103014473-103014492	None:intergenic	35.0%
	TTGTTAACACTCCTAATGCA+AGG	+	Chr6:103014459-103014478	MsG0680035349.01.T01:CDS	35.0%
!	GATTCTTCATGTATTGGCAA+AGG	-	Chr6:103014424-103014443	None:intergenic	35.0%
!!	AGGTCTTTGCTCTTTGATTT+TGG	-	Chr6:103014399-103014418	None:intergenic	35.0%
	AACCAAAAGCAGCAAAAGGA+AGG	-	Chr6:103014276-103014295	None:intergenic	40.0%
	AGGAAAGTTGCTAACACTTG+TGG	+	Chr6:103014479-103014498	MsG0680035349.01.T01:CDS	40.0%
!	TTCATGTATTGGCAAAGGCA+AGG	-	Chr6:103014419-103014438	None:intergenic	40.0%
!!!	CTTTGATTTTGGAGCAGCAT+AGG	-	Chr6:103014388-103014407	None:intergenic	40.0%
	TTGGAGCAGCATAGGTTAGA+TGG	-	Chr6:103014380-103014399	None:intergenic	45.0%
!	CTTGTGGAACTCCTTTTCCA+AGG	+	Chr6:103014495-103014514	MsG0680035349.01.T01:CDS	45.0%
!!!	CACCTTCCTTTTGCTGCTTT+TGG	+	Chr6:103014271-103014290	MsG0680035349.01.T01:CDS	45.0%
	GAGCAGCATAGGTTAGATGG+AGG	-	Chr6:103014377-103014396	None:intergenic	50.0%
	ACACATGAGCTGAGCTGCAC+TGG	-	Chr6:103014332-103014351	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	103014245	103014523	103014245	ID=MsG0680035349.01;Name=MsG0680035349.01
Chr6	mRNA	103014245	103014523	103014245	ID=MsG0680035349.01.T01;Parent=MsG0680035349.01;Name=MsG0680035349.01.T01;_AED=0.35;_eAED=0.35;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|92
Chr6	exon	103014245	103014523	103014245	ID=MsG0680035349.01.T01:exon:17211;Parent=MsG0680035349.01.T01
Chr6	CDS	103014245	103014523	103014245	ID=MsG0680035349.01.T01:cds;Parent=MsG0680035349.01.T01
Chr6	mRNA	103014311	103014523	103014311	ID=MsG0680035349.01.T02;Parent=MsG0680035349.01;Name=MsG0680035349.01.T02;_AED=0.39;_eAED=0.39;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|70
Chr6	exon	103014311	103014523	103014311	ID=MsG0680035349.01.T02:exon:17212;Parent=MsG0680035349.01.T02
Chr6	CDS	103014311	103014523	103014311	ID=MsG0680035349.01.T02:cds;Parent=MsG0680035349.01.T02
Chr6	mRNA	103014434	103014523	103014434	ID=MsG0680035349.01.T03;Parent=MsG0680035349.01;Name=MsG0680035349.01.T03;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|29
Chr6	exon	103014434	103014523	103014434	ID=MsG0680035349.01.T03:exon:17213;Parent=MsG0680035349.01.T03
Chr6	CDS	103014434	103014523	103014434	ID=MsG0680035349.01.T03:cds;Parent=MsG0680035349.01.T03

Gene Sequence

>MsG0680035349.01.T01

ATGAAAGCATCACAAATTGCTATTTGCACCTTCCTTTTGCTGCTTTTGGTTAATGTTCAAGTGTCAATGGCAGTAACATGCAGTCCAGTGCAGCTCAGCTCATGTGTGAGTGCAATCACATCTTCAACTCCTCCATCTAACCTATGCTGCTCCAAAATCAAAGAGCAAAGACCTTGCCTTTGCCAATACATGAAGAATCCCAATCTTAGGAAGTTTGTTAACACTCCTAATGCAAGGAAAGTTGCTAACACTTGTGGAACTCCTTTTCCAAGGTGTTAA

>MsG0680035349.01.T03

ATGAAGAATCCCAATCTTAGGAAGTTTGTTAACACTCCTAATGCAAGGAAAGTTGCTAACACTTGTGGAACTCCTTTTCCAAGGTGTTAA

>MsG0680035349.01.T02

ATGGCAGTAACATGCAGTCCAGTGCAGCTCAGCTCATGTGTGAGTGCAATCACATCTTCAACTCCTCCATCTAACCTATGCTGCTCCAAAATCAAAGAGCAAAGACCTTGCCTTTGCCAATACATGAAGAATCCCAATCTTAGGAAGTTTGTTAACACTCCTAATGCAAGGAAAGTTGCTAACACTTGTGGAACTCCTTTTCCAAGGTGTTAA

Protein sequence

>MsG0680035349.01.T01

MKASQIAICTFLLLLLVNVQVSMAVTCSPVQLSSCVSAITSSTPPSNLCCSKIKEQRPCLCQYMKNPNLRKFVNTPNARKVANTCGTPFPRC*

>MsG0680035349.01.T03

MKNPNLRKFVNTPNARKVANTCGTPFPRC*

>MsG0680035349.01.T02

MAVTCSPVQLSSCVSAITSSTPPSNLCCSKIKEQRPCLCQYMKNPNLRKFVNTPNARKVANTCGTPFPRC*