Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036666.01.T01 | XP_039684161.1 | 43.22 | 236 | 52 | 6 | 1 | 158 | 237 | 468 | 1.21E-41 | 157 |
| MsG0780036666.01.T01 | XP_039684161.1 | 100 | 35 | 0 | 0 | 1 | 35 | 134 | 168 | 7.30E-13 | 77.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036666.01.T01 | Q9STF3 | 32.934 | 167 | 62 | 4 | 48 | 165 | 430 | 595 | 1.15E-22 | 97.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036666.01.T01 | Q2HSF0 | 43.220 | 236 | 52 | 6 | 1 | 158 | 354 | 585 | 3.33e-41 | 157 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048849.01 | MsG0780036666.01 | 0.817122 | 3.883448e-52 | 2.803705e-49 |
| MsG0080049069.01 | MsG0780036666.01 | 0.814664 | 1.373288e-51 | 9.270759e-49 |
| MsG0180001548.01 | MsG0780036666.01 | 0.847556 | 1.073466e-59 | 1.915300e-56 |
| MsG0180001704.01 | MsG0780036666.01 | 0.807071 | 6.046690e-50 | 3.335830e-47 |
| MsG0180002184.01 | MsG0780036666.01 | 0.807452 | 5.020317e-50 | 2.797334e-47 |
| MsG0180004025.01 | MsG0780036666.01 | 0.809530 | 1.807847e-50 | 1.064062e-47 |
| MsG0180005005.01 | MsG0780036666.01 | 0.821168 | 4.660275e-53 | 3.763018e-50 |
| MsG0180006055.01 | MsG0780036666.01 | 0.811719 | 6.083512e-51 | 3.793692e-48 |
| MsG0380012868.01 | MsG0780036666.01 | 0.812134 | 4.939244e-51 | 3.114781e-48 |
| MsG0480019384.01 | MsG0780036666.01 | 0.836633 | 8.345243e-57 | 1.057766e-53 |
| MsG0480021201.01 | MsG0780036666.01 | 0.821776 | 3.372146e-53 | 2.769770e-50 |
| MsG0480023449.01 | MsG0780036666.01 | 0.823356 | 1.447816e-53 | 1.243612e-50 |
| MsG0580025280.01 | MsG0780036666.01 | 0.802883 | 4.542072e-49 | 2.250266e-46 |
| MsG0580025564.01 | MsG0780036666.01 | 0.829464 | 5.072397e-55 | 5.195434e-52 |
| MsG0380017793.01 | MsG0780036666.01 | 0.806235 | 9.075812e-50 | 4.900992e-47 |
| MsG0480018426.01 | MsG0780036666.01 | 0.841632 | 4.224159e-58 | 6.244548e-55 |
| MsG0580029511.01 | MsG0780036666.01 | 0.845868 | 3.104765e-59 | 5.246881e-56 |
| MsG0280007647.01 | MsG0780036666.01 | 0.813827 | 2.101576e-51 | 1.386953e-48 |
| MsG0780036666.01 | MsG0780041747.01 | 0.805964 | 1.035374e-49 | 5.552310e-47 |
| MsG0780036666.01 | MsG0880042512.01 | 0.855590 | 5.695634e-62 | 1.321618e-58 |
| MsG0780036666.01 | MsG0880043024.01 | 0.812919 | 3.326956e-51 | 2.142245e-48 |
| MsG0780036666.01 | MsG0880045312.01 | 0.850405 | 1.734616e-60 | 3.394238e-57 |
| MsG0780036666.01 | MsG0880046536.01 | 0.819912 | 9.049868e-53 | 7.057284e-50 |
| MsG0780036666.01 | MsG0880046930.01 | 0.801126 | 1.042832e-48 | 4.941366e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036666.01.T01 | MTR_7g016960 | 43.220 | 236 | 52 | 6 | 1 | 158 | 354 | 585 | 8.44e-45 | 157 |
| MsG0780036666.01.T01 | MTR_7g016960 | 100.000 | 35 | 0 | 0 | 1 | 35 | 251 | 285 | 9.98e-17 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036666.01.T01 | AT3G46790 | 32.934 | 167 | 62 | 4 | 48 | 165 | 430 | 595 | 1.17e-23 | 97.8 |
| MsG0780036666.01.T01 | AT3G57430 | 28.889 | 135 | 78 | 1 | 31 | 165 | 712 | 828 | 8.65e-11 | 60.5 |
Find 37 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGAATTAATACAAAATA+TGG | 0.224923 | 7:+11550621 | MsG0780036666.01.T01:CDS |
| AGGACACTCACCATTGTTAT+TGG | 0.229883 | 7:-11550455 | None:intergenic |
| CTTCAAAAGGTGAAATCATT+AGG | 0.289637 | 7:+11551095 | MsG0780036666.01.T01:CDS |
| CAAGCATAATGCTCCATTCT+TGG | 0.292157 | 7:-11550557 | None:intergenic |
| GGAAAGCTTGCCGTTGCTTT+CGG | 0.323608 | 7:+11551061 | MsG0780036666.01.T01:CDS |
| AAAAGTTCCAATGCTTTCAT+AGG | 0.365010 | 7:-11550392 | None:intergenic |
| CACGAAGCTCTTCGATTTGT+GGG | 0.367803 | 7:-11550923 | None:intergenic |
| CTCACCATTGTTATTGGATT+CGG | 0.374114 | 7:-11550449 | None:intergenic |
| TCTCGGTTAACAGTGTAATA+AGG | 0.382822 | 7:-11550950 | None:intergenic |
| GTTAACCGAGATCAAGAATC+AGG | 0.383390 | 7:+11550962 | MsG0780036666.01.T01:CDS |
| ATATTCTTGGCCGAGCTAAT+CGG | 0.391209 | 7:+11550587 | MsG0780036666.01.T01:CDS |
| TGCTTGTATGGTGATATTCT+TGG | 0.401200 | 7:+11550574 | MsG0780036666.01.T01:CDS |
| AGAGAAGGAGAAGAATTGTA+TGG | 0.402534 | 7:+11551031 | MsG0780036666.01.T01:CDS |
| GGACTCATTAATACTTCAAA+AGG | 0.423177 | 7:+11551082 | MsG0780036666.01.T01:CDS |
| CAAAATATGGATTTCAAACC+TGG | 0.433346 | 7:+11550634 | MsG0780036666.01.T01:CDS |
| GAAGAATTGTATGGGAATAG+TGG | 0.452202 | 7:+11551040 | MsG0780036666.01.T01:CDS |
| GCAAAAGATTCCGTTGCAGC+TGG | 0.452531 | 7:+11550850 | MsG0780036666.01.T01:CDS |
| GCACGAAGCTCTTCGATTTG+TGG | 0.453741 | 7:-11550924 | None:intergenic |
| CTAGGATGTGGAACGATGTA+AGG | 0.464429 | 7:+11550797 | MsG0780036666.01.T01:intron |
| TGAAATGCCTATGAAAGCAT+TGG | 0.484672 | 7:+11550385 | MsG0780036666.01.T01:CDS |
| AATGGAGCATTATGCTTGTA+TGG | 0.495835 | 7:+11550562 | MsG0780036666.01.T01:CDS |
| GAACCCCAAACCGTTGGTCC+AGG | 0.496892 | 7:-11550652 | None:intergenic |
| GCAGCTGGATTGAAGTCAAA+AGG | 0.502973 | 7:+11550865 | MsG0780036666.01.T01:CDS |
| CATAATGCTCCATTCTTGGA+TGG | 0.504654 | 7:-11550553 | None:intergenic |
| TGACTTCAATCCAGCTGCAA+CGG | 0.516833 | 7:-11550860 | None:intergenic |
| GAGAAGGAGAAGAATTGTAT+GGG | 0.539879 | 7:+11551032 | MsG0780036666.01.T01:CDS |
| GTACAGAATCCATCCAAGAA+TGG | 0.540687 | 7:+11550544 | MsG0780036666.01.T01:intron |
| CGATCTCGATGAAGAAGAGA+AGG | 0.551474 | 7:+11551016 | MsG0780036666.01.T01:CDS |
| CTTCATCAAACCGATTAGCT+CGG | 0.556750 | 7:-11550597 | None:intergenic |
| ATAACCCTGATTCTTGATCT+CGG | 0.565988 | 7:-11550967 | None:intergenic |
| GGATTTCAAACCTGGACCAA+CGG | 0.587827 | 7:+11550642 | MsG0780036666.01.T01:CDS |
| TAAGTAACAACGTTTGTCTG+TGG | 0.609575 | 7:-11550993 | None:intergenic |
| ATTAATGAGTCCGAAAGCAA+CGG | 0.620662 | 7:-11551071 | None:intergenic |
| TTAACCGAGATCAAGAATCA+GGG | 0.657230 | 7:+11550963 | MsG0780036666.01.T01:CDS |
| TAAGGCGTGTGAGGAAACAA+TGG | 0.668162 | 7:+11550815 | MsG0780036666.01.T01:CDS |
| AGTTCCGAATCCAATAACAA+TGG | 0.689702 | 7:+11550445 | MsG0780036666.01.T01:CDS |
| GGAACGATGTAAGGCGTGTG+AGG | 0.696311 | 7:+11550806 | MsG0780036666.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAATTAATACAAAATA+TGG | + | Chr7:11550621-11550640 | MsG0780036666.01.T01:CDS | 10.0% |
| AAAAGTTCCAATGCTTTCAT+AGG | - | Chr7:11550395-11550414 | None:intergenic | 30.0% | |
| CAAAATATGGATTTCAAACC+TGG | + | Chr7:11550634-11550653 | MsG0780036666.01.T01:CDS | 30.0% | |
| CTTCAAAAGGTGAAATCATT+AGG | + | Chr7:11551095-11551114 | MsG0780036666.01.T01:CDS | 30.0% | |
| GGACTCATTAATACTTCAAA+AGG | + | Chr7:11551082-11551101 | MsG0780036666.01.T01:CDS | 30.0% | |
| TCAGATATTACGCAAAATCT+AGG | + | Chr7:11550779-11550798 | MsG0780036666.01.T01:intron | 30.0% | |
| ! | TTTTCAGATAATGATGCTTG+AGG | + | Chr7:11550412-11550431 | MsG0780036666.01.T01:CDS | 30.0% |
| AAATCTTTGCCTCTTCAACA+AGG | - | Chr7:11550502-11550521 | None:intergenic | 35.0% | |
| AGAGAAGGAGAAGAATTGTA+TGG | + | Chr7:11551031-11551050 | MsG0780036666.01.T01:CDS | 35.0% | |
| AGTTCCGAATCCAATAACAA+TGG | + | Chr7:11550445-11550464 | MsG0780036666.01.T01:CDS | 35.0% | |
| ATAACCCTGATTCTTGATCT+CGG | - | Chr7:11550970-11550989 | None:intergenic | 35.0% | |
| ATTAATGAGTCCGAAAGCAA+CGG | - | Chr7:11551074-11551093 | None:intergenic | 35.0% | |
| GAAGAATTGTATGGGAATAG+TGG | + | Chr7:11551040-11551059 | MsG0780036666.01.T01:CDS | 35.0% | |
| GAGAAGGAGAAGAATTGTAT+GGG | + | Chr7:11551032-11551051 | MsG0780036666.01.T01:CDS | 35.0% | |
| TAAGTAACAACGTTTGTCTG+TGG | - | Chr7:11550996-11551015 | None:intergenic | 35.0% | |
| TCTCGGTTAACAGTGTAATA+AGG | - | Chr7:11550953-11550972 | None:intergenic | 35.0% | |
| TGCTTGTATGGTGATATTCT+TGG | + | Chr7:11550574-11550593 | MsG0780036666.01.T01:CDS | 35.0% | |
| TTAACCGAGATCAAGAATCA+GGG | + | Chr7:11550963-11550982 | MsG0780036666.01.T01:CDS | 35.0% | |
| ! | AATGGAGCATTATGCTTGTA+TGG | + | Chr7:11550562-11550581 | MsG0780036666.01.T01:CDS | 35.0% |
| ! | ATTACGCAAAATCTAGGATG+TGG | + | Chr7:11550785-11550804 | MsG0780036666.01.T01:intron | 35.0% |
| ! | CTCACCATTGTTATTGGATT+CGG | - | Chr7:11550452-11550471 | None:intergenic | 35.0% |
| ! | TGAAATGCCTATGAAAGCAT+TGG | + | Chr7:11550385-11550404 | MsG0780036666.01.T01:CDS | 35.0% |
| ACGTAATTGCCAGCATTCTT+AGG | - | Chr7:11550753-11550772 | None:intergenic | 40.0% | |
| AGGACACTCACCATTGTTAT+TGG | - | Chr7:11550458-11550477 | None:intergenic | 40.0% | |
| ATATTCTTGGCCGAGCTAAT+CGG | + | Chr7:11550587-11550606 | MsG0780036666.01.T01:CDS | 40.0% | |
| CAAGCATAATGCTCCATTCT+TGG | - | Chr7:11550560-11550579 | None:intergenic | 40.0% | |
| CATAATGCTCCATTCTTGGA+TGG | - | Chr7:11550556-11550575 | None:intergenic | 40.0% | |
| GTACAGAATCCATCCAAGAA+TGG | + | Chr7:11550544-11550563 | MsG0780036666.01.T01:intron | 40.0% | |
| GTTAACCGAGATCAAGAATC+AGG | + | Chr7:11550962-11550981 | MsG0780036666.01.T01:CDS | 40.0% | |
| ! | AAGCGCTATGCTATTTGAGT+TGG | + | Chr7:11550725-11550744 | MsG0780036666.01.T01:intron | 40.0% |
| ! | CTTCATCAAACCGATTAGCT+CGG | - | Chr7:11550600-11550619 | None:intergenic | 40.0% |
| CGATCTCGATGAAGAAGAGA+AGG | + | Chr7:11551016-11551035 | MsG0780036666.01.T01:CDS | 45.0% | |
| CTAGGATGTGGAACGATGTA+AGG | + | Chr7:11550797-11550816 | MsG0780036666.01.T01:intron | 45.0% | |
| GCAGCTGGATTGAAGTCAAA+AGG | + | Chr7:11550865-11550884 | MsG0780036666.01.T01:CDS | 45.0% | |
| GGATTTCAAACCTGGACCAA+CGG | + | Chr7:11550642-11550661 | MsG0780036666.01.T01:CDS | 45.0% | |
| GGTTCGCTTCTTGATCTTGT+AGG | + | Chr7:11550670-11550689 | MsG0780036666.01.T01:intron | 45.0% | |
| TAAGGCGTGTGAGGAAACAA+TGG | + | Chr7:11550815-11550834 | MsG0780036666.01.T01:CDS | 45.0% | |
| TGACTTCAATCCAGCTGCAA+CGG | - | Chr7:11550863-11550882 | None:intergenic | 45.0% | |
| ! | CACGAAGCTCTTCGATTTGT+GGG | - | Chr7:11550926-11550945 | None:intergenic | 45.0% |
| AAACCTGGACCAACGGTTTG+GGG | + | Chr7:11550649-11550668 | MsG0780036666.01.T01:intron | 50.0% | |
| CAAACCTGGACCAACGGTTT+GGG | + | Chr7:11550648-11550667 | MsG0780036666.01.T01:intron | 50.0% | |
| GAGTTGGAGCCTAAGAATGC+TGG | + | Chr7:11550741-11550760 | MsG0780036666.01.T01:intron | 50.0% | |
| GCAAAAGATTCCGTTGCAGC+TGG | + | Chr7:11550850-11550869 | MsG0780036666.01.T01:CDS | 50.0% | |
| TCAAACCTGGACCAACGGTT+TGG | + | Chr7:11550647-11550666 | MsG0780036666.01.T01:intron | 50.0% | |
| ! | GCACGAAGCTCTTCGATTTG+TGG | - | Chr7:11550927-11550946 | None:intergenic | 50.0% |
| ! | GGAAAGCTTGCCGTTGCTTT+CGG | + | Chr7:11551061-11551080 | MsG0780036666.01.T01:CDS | 50.0% |
| AGAAGCGAACCCCAAACCGT+TGG | - | Chr7:11550661-11550680 | None:intergenic | 55.0% | |
| CCTCTTCAACAAGGCCTGCA+TGG | - | Chr7:11550493-11550512 | None:intergenic | 55.0% | |
| CTGCATGGCTGCAAGCTTGA+AGG | - | Chr7:11550478-11550497 | None:intergenic | 55.0% | |
| CTTCAAGCTTGCAGCCATGC+AGG | + | Chr7:11550476-11550495 | MsG0780036666.01.T01:intron | 55.0% | |
| GGAACGATGTAAGGCGTGTG+AGG | + | Chr7:11550806-11550825 | MsG0780036666.01.T01:CDS | 55.0% | |
| !! | CCATGCAGGCCTTGTTGAAG+AGG | + | Chr7:11550490-11550509 | MsG0780036666.01.T01:intron | 55.0% |
| GAACCCCAAACCGTTGGTCC+AGG | - | Chr7:11550655-11550674 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 11550362 | 11551142 | 11550362 | ID=MsG0780036666.01;Name=MsG0780036666.01 |
| Chr7 | mRNA | 11550362 | 11551142 | 11550362 | ID=MsG0780036666.01.T01;Parent=MsG0780036666.01;Name=MsG0780036666.01.T01;_AED=0.42;_eAED=0.47;_QI=0|0|0|1|1|1|3|0|186 |
| Chr7 | exon | 11550362 | 11550466 | 11550362 | ID=MsG0780036666.01.T01:exon:2143;Parent=MsG0780036666.01.T01 |
| Chr7 | exon | 11550550 | 11550663 | 11550550 | ID=MsG0780036666.01.T01:exon:2144;Parent=MsG0780036666.01.T01 |
| Chr7 | exon | 11550801 | 11551142 | 11550801 | ID=MsG0780036666.01.T01:exon:2145;Parent=MsG0780036666.01.T01 |
| Chr7 | CDS | 11550362 | 11550466 | 11550362 | ID=MsG0780036666.01.T01:cds;Parent=MsG0780036666.01.T01 |
| Chr7 | CDS | 11550550 | 11550663 | 11550550 | ID=MsG0780036666.01.T01:cds;Parent=MsG0780036666.01.T01 |
| Chr7 | CDS | 11550801 | 11551142 | 11550801 | ID=MsG0780036666.01.T01:cds;Parent=MsG0780036666.01.T01 |
| Gene Sequence |
| Protein sequence |
