Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037047.01.T01 | XP_003611244.1 | 89.286 | 56 | 6 | 0 | 47 | 102 | 84 | 139 | 2.00E-25 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037047.01.T01 | Q94AZ4 | 76.786 | 56 | 13 | 0 | 47 | 102 | 85 | 140 | 2.08E-23 | 91.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037047.01.T01 | B7FNA2 | 89.286 | 56 | 6 | 0 | 47 | 102 | 84 | 139 | 9.56e-26 | 103 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000470.01 | MsG0780037047.01 | 0.818690 | 1.718150e-52 | 1.295050e-49 |
| MsG0180003673.01 | MsG0780037047.01 | 0.800283 | 1.549978e-48 | 7.188938e-46 |
| MsG0180003686.01 | MsG0780037047.01 | 0.810989 | 8.758866e-51 | 5.358715e-48 |
| MsG0180004275.01 | MsG0780037047.01 | 0.810681 | 1.021348e-50 | 6.196749e-48 |
| MsG0180005185.01 | MsG0780037047.01 | 0.807154 | 5.805241e-50 | 3.209634e-47 |
| MsG0180006134.01 | MsG0780037047.01 | 0.801291 | 9.651670e-49 | 4.592269e-46 |
| MsG0280010698.01 | MsG0780037047.01 | 0.810358 | 1.199621e-50 | 7.215762e-48 |
| MsG0380012947.01 | MsG0780037047.01 | 0.841265 | 5.275566e-58 | 7.709682e-55 |
| MsG0680030880.01 | MsG0780037047.01 | 0.815142 | 1.075767e-51 | 7.356453e-49 |
| MsG0480018653.01 | MsG0780037047.01 | 0.800481 | 1.412310e-48 | 6.583160e-46 |
| MsG0480020836.01 | MsG0780037047.01 | 0.800261 | 1.566181e-48 | 7.259848e-46 |
| MsG0480022068.01 | MsG0780037047.01 | 0.805727 | 1.160801e-49 | 6.187476e-47 |
| MsG0380015039.01 | MsG0780037047.01 | 0.810320 | 1.222414e-50 | 7.345426e-48 |
| MsG0480018236.01 | MsG0780037047.01 | 0.801834 | 7.467548e-49 | 3.602156e-46 |
| MsG0580027018.01 | MsG0780037047.01 | 0.816074 | 6.669354e-52 | 4.679225e-49 |
| MsG0280010349.01 | MsG0780037047.01 | 0.810786 | 9.692656e-51 | 5.897314e-48 |
| MsG0780036018.01 | MsG0780037047.01 | 0.825922 | 3.599793e-54 | 3.326775e-51 |
| MsG0780036201.01 | MsG0780037047.01 | 0.816994 | 4.150108e-52 | 2.985997e-49 |
| MsG0780037047.01 | MsG0780038439.01 | 0.815074 | 1.113485e-51 | 7.600637e-49 |
| MsG0780037047.01 | MsG0780039171.01 | 0.803002 | 4.291312e-49 | 2.132390e-46 |
| MsG0780037047.01 | MsG0780040074.01 | 0.808128 | 3.605934e-50 | 2.045075e-47 |
| MsG0780037047.01 | MsG0880043941.01 | 0.802748 | 4.842694e-49 | 2.391061e-46 |
| MsG0780037047.01 | MsG0880045372.01 | 0.809048 | 2.293455e-50 | 1.332602e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037047.01.T01 | MTR_5g011850 | 89.286 | 56 | 6 | 0 | 47 | 102 | 84 | 139 | 2.42e-29 | 103 |
| MsG0780037047.01.T01 | MTR_0168s0120 | 42.105 | 76 | 41 | 1 | 27 | 102 | 33 | 105 | 5.56e-16 | 68.9 |
| MsG0780037047.01.T01 | MTR_6g025320 | 42.105 | 76 | 41 | 1 | 27 | 102 | 33 | 105 | 5.56e-16 | 68.9 |
| MsG0780037047.01.T01 | MTR_5g088320 | 42.105 | 76 | 41 | 1 | 27 | 102 | 69 | 141 | 1.06e-15 | 69.3 |
| MsG0780037047.01.T01 | MTR_6g025320 | 42.105 | 76 | 41 | 1 | 27 | 102 | 69 | 141 | 1.10e-15 | 69.3 |
| MsG0780037047.01.T01 | MTR_7g087610 | 41.333 | 75 | 41 | 1 | 27 | 101 | 69 | 140 | 3.21e-15 | 67.8 |
| MsG0780037047.01.T01 | MTR_1g041285 | 32.895 | 76 | 48 | 1 | 27 | 102 | 69 | 141 | 1.03e-11 | 58.9 |
| MsG0780037047.01.T01 | MTR_1g076650 | 31.579 | 76 | 49 | 1 | 27 | 102 | 69 | 141 | 2.78e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037047.01.T01 | AT1G12310 | 76.786 | 56 | 13 | 0 | 47 | 102 | 85 | 140 | 2.12e-24 | 91.7 |
| MsG0780037047.01.T01 | AT1G62820 | 75.000 | 56 | 14 | 0 | 47 | 102 | 85 | 140 | 2.14e-24 | 91.7 |
| MsG0780037047.01.T01 | AT3G43810 | 42.105 | 76 | 41 | 1 | 27 | 102 | 33 | 105 | 5.83e-16 | 68.9 |
| MsG0780037047.01.T01 | AT3G43810 | 42.105 | 76 | 41 | 1 | 27 | 102 | 33 | 105 | 5.83e-16 | 68.9 |
| MsG0780037047.01.T01 | AT5G21274 | 42.105 | 76 | 41 | 1 | 27 | 102 | 69 | 141 | 1.01e-15 | 69.3 |
| MsG0780037047.01.T01 | AT3G43810 | 42.105 | 76 | 41 | 1 | 27 | 102 | 69 | 141 | 1.16e-15 | 69.3 |
| MsG0780037047.01.T01 | AT3G56800 | 40.789 | 76 | 42 | 1 | 27 | 102 | 69 | 141 | 4.35e-15 | 67.8 |
| MsG0780037047.01.T01 | AT2G27030 | 40.789 | 76 | 42 | 1 | 27 | 102 | 69 | 141 | 4.35e-15 | 67.8 |
| MsG0780037047.01.T01 | AT2G41110 | 40.789 | 76 | 42 | 1 | 27 | 102 | 69 | 141 | 4.35e-15 | 67.8 |
| MsG0780037047.01.T01 | AT2G27030 | 40.789 | 76 | 42 | 1 | 27 | 102 | 69 | 141 | 7.14e-15 | 67.8 |
| MsG0780037047.01.T01 | AT3G43810 | 42.105 | 76 | 41 | 1 | 27 | 102 | 69 | 141 | 9.68e-15 | 68.2 |
| MsG0780037047.01.T01 | AT1G66410 | 39.474 | 76 | 43 | 1 | 27 | 102 | 33 | 105 | 1.40e-14 | 65.5 |
| MsG0780037047.01.T01 | AT1G66410 | 39.474 | 76 | 43 | 1 | 27 | 102 | 33 | 105 | 1.40e-14 | 65.5 |
| MsG0780037047.01.T01 | AT2G41110 | 40.789 | 76 | 42 | 1 | 27 | 102 | 81 | 153 | 1.96e-14 | 66.2 |
| MsG0780037047.01.T01 | AT5G37780 | 39.474 | 76 | 43 | 1 | 27 | 102 | 69 | 141 | 2.48e-14 | 65.9 |
| MsG0780037047.01.T01 | AT1G66410 | 39.474 | 76 | 43 | 1 | 27 | 102 | 69 | 141 | 2.48e-14 | 65.9 |
| MsG0780037047.01.T01 | AT5G37780 | 39.474 | 76 | 43 | 1 | 27 | 102 | 56 | 128 | 2.87e-14 | 65.1 |
| MsG0780037047.01.T01 | AT1G66410 | 39.474 | 76 | 43 | 1 | 27 | 102 | 79 | 151 | 3.07e-14 | 65.9 |
| MsG0780037047.01.T01 | AT5G37780 | 39.474 | 76 | 43 | 1 | 27 | 102 | 84 | 156 | 3.49e-14 | 65.5 |
| MsG0780037047.01.T01 | AT5G37780 | 39.474 | 76 | 43 | 1 | 27 | 102 | 95 | 167 | 5.58e-14 | 65.5 |
| MsG0780037047.01.T01 | AT3G22930 | 41.071 | 56 | 33 | 0 | 47 | 102 | 109 | 164 | 2.35e-11 | 58.5 |
| MsG0780037047.01.T01 | AT4G14640 | 32.895 | 76 | 48 | 1 | 27 | 102 | 70 | 142 | 4.58e-11 | 57.4 |
| MsG0780037047.01.T01 | AT4G14640 | 32.895 | 76 | 48 | 1 | 27 | 102 | 52 | 124 | 5.02e-11 | 56.6 |
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAGTTTGATGAATGTATT+AGG | 0.235303 | 7:+18549747 | MsG0780037047.01.T01:CDS |
| CGATGGATTTGAGTTGGGCT+TGG | 0.312614 | 7:-18549606 | None:intergenic |
| TGCGATCTTGCCGTCGTTGT+CGG | 0.415377 | 7:-18549536 | None:intergenic |
| ATTTGAGTTGGGCTTGGGTC+GGG | 0.416027 | 7:-18549600 | None:intergenic |
| CGGCGACGATGGATTTGAGT+TGG | 0.421727 | 7:-18549612 | None:intergenic |
| GATGGATTTGAGTTGGGCTT+GGG | 0.428812 | 7:-18549605 | None:intergenic |
| GATTTGAGTTGGGCTTGGGT+CGG | 0.446248 | 7:-18549601 | None:intergenic |
| GGCGACGATGGATTTGAGTT+GGG | 0.457758 | 7:-18549611 | None:intergenic |
| GTTCTCGATAAAGATGCTAC+TGG | 0.465422 | 7:+18549662 | MsG0780037047.01.T01:CDS |
| GAAATCGAAAGGAGAACATT+CGG | 0.467430 | 7:-18549632 | None:intergenic |
| AGGAGAACATTCGGCGACGA+TGG | 0.475961 | 7:-18549623 | None:intergenic |
| TGAGTTTGATGAATGTATTA+GGG | 0.475992 | 7:+18549748 | MsG0780037047.01.T01:CDS |
| GAAGTTGATGTTGGGTCTGA+TGG | 0.478332 | 7:+18549770 | MsG0780037047.01.T01:CDS |
| GGAATCTTGTTGCTTTCTCT+CGG | 0.491374 | 7:+18549571 | MsG0780037047.01.T01:CDS |
| TGTATTAGGGAAGTTGATGT+TGG | 0.500218 | 7:+18549761 | MsG0780037047.01.T01:CDS |
| AAGCAATTGCATTCATCACT+TGG | 0.500551 | 7:-18549830 | None:intergenic |
| ATGAAGATTCATCGCAAGAA+TGG | 0.516523 | 7:+18549804 | MsG0780037047.01.T01:CDS |
| ATACATTCATCAAACTCAGC+AGG | 0.529884 | 7:-18549743 | None:intergenic |
| GTATTAGGGAAGTTGATGTT+GGG | 0.530223 | 7:+18549762 | MsG0780037047.01.T01:CDS |
| AAGTTGATGTTGGGTCTGAT+GGG | 0.550763 | 7:+18549771 | MsG0780037047.01.T01:CDS |
| CTTGCGATGAATCTTCATAA+CGG | 0.557826 | 7:-18549799 | None:intergenic |
| CGACGGCAAGATCGCACCAT+CGG | 0.559292 | 7:+18549543 | MsG0780037047.01.T01:CDS |
| AAGATCGCACCATCGGAGCT+CGG | 0.573545 | 7:+18549550 | MsG0780037047.01.T01:CDS |
| GAACTTTGAGAGAAATCGAA+AGG | 0.580421 | 7:-18549643 | None:intergenic |
| AACAAGATTCCGAGCTCCGA+TGG | 0.595819 | 7:-18549559 | None:intergenic |
| ATCTTGTTGCTTTCTCTCGG+CGG | 0.608961 | 7:+18549574 | MsG0780037047.01.T01:CDS |
| CTCTTCGACACCGACAACGA+CGG | 0.661849 | 7:+18549526 | MsG0780037047.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCTTTTTATATTTTTC+TGG | + | Chr7:18549845-18549864 | MsG0780037047.01.T01:CDS | 15.0% |
| ! | TGAGTTTGATGAATGTATTA+GGG | + | Chr7:18549748-18549767 | MsG0780037047.01.T01:CDS | 25.0% |
| CTGAGTTTGATGAATGTATT+AGG | + | Chr7:18549747-18549766 | MsG0780037047.01.T01:CDS | 30.0% | |
| !!! | CATCATATTTTGACGAGTAT+TGG | + | Chr7:18549707-18549726 | MsG0780037047.01.T01:CDS | 30.0% |
| AAGCAATTGCATTCATCACT+TGG | - | Chr7:18549833-18549852 | None:intergenic | 35.0% | |
| ATACATTCATCAAACTCAGC+AGG | - | Chr7:18549746-18549765 | None:intergenic | 35.0% | |
| ATGAAGATTCATCGCAAGAA+TGG | + | Chr7:18549804-18549823 | MsG0780037047.01.T01:CDS | 35.0% | |
| CTTGCGATGAATCTTCATAA+CGG | - | Chr7:18549802-18549821 | None:intergenic | 35.0% | |
| GAAATCGAAAGGAGAACATT+CGG | - | Chr7:18549635-18549654 | None:intergenic | 35.0% | |
| ! | GAACTTTGAGAGAAATCGAA+AGG | - | Chr7:18549646-18549665 | None:intergenic | 35.0% |
| !! | GTATTAGGGAAGTTGATGTT+GGG | + | Chr7:18549762-18549781 | MsG0780037047.01.T01:CDS | 35.0% |
| !! | TGTATTAGGGAAGTTGATGT+TGG | + | Chr7:18549761-18549780 | MsG0780037047.01.T01:CDS | 35.0% |
| GTTCTCGATAAAGATGCTAC+TGG | + | Chr7:18549662-18549681 | MsG0780037047.01.T01:CDS | 40.0% | |
| !! | AAGTTGATGTTGGGTCTGAT+GGG | + | Chr7:18549771-18549790 | MsG0780037047.01.T01:CDS | 40.0% |
| !! | GGAATCTTGTTGCTTTCTCT+CGG | + | Chr7:18549571-18549590 | MsG0780037047.01.T01:CDS | 40.0% |
| !! | ATCTTGTTGCTTTCTCTCGG+CGG | + | Chr7:18549574-18549593 | MsG0780037047.01.T01:CDS | 45.0% |
| !! | GAAGTTGATGTTGGGTCTGA+TGG | + | Chr7:18549770-18549789 | MsG0780037047.01.T01:CDS | 45.0% |
| !! | GATGGATTTGAGTTGGGCTT+GGG | - | Chr7:18549608-18549627 | None:intergenic | 45.0% |
| AACAAGATTCCGAGCTCCGA+TGG | - | Chr7:18549562-18549581 | None:intergenic | 50.0% | |
| ! | GGCGACGATGGATTTGAGTT+GGG | - | Chr7:18549614-18549633 | None:intergenic | 50.0% |
| !! | ATTTGAGTTGGGCTTGGGTC+GGG | - | Chr7:18549603-18549622 | None:intergenic | 50.0% |
| !! | CGATGGATTTGAGTTGGGCT+TGG | - | Chr7:18549609-18549628 | None:intergenic | 50.0% |
| !! | GATTTGAGTTGGGCTTGGGT+CGG | - | Chr7:18549604-18549623 | None:intergenic | 50.0% |
| AAGATCGCACCATCGGAGCT+CGG | + | Chr7:18549550-18549569 | MsG0780037047.01.T01:CDS | 55.0% | |
| AGGAGAACATTCGGCGACGA+TGG | - | Chr7:18549626-18549645 | None:intergenic | 55.0% | |
| CTCTTCGACACCGACAACGA+CGG | + | Chr7:18549526-18549545 | MsG0780037047.01.T01:CDS | 55.0% | |
| TGCGATCTTGCCGTCGTTGT+CGG | - | Chr7:18549539-18549558 | None:intergenic | 55.0% | |
| ! | CGGCGACGATGGATTTGAGT+TGG | - | Chr7:18549615-18549634 | None:intergenic | 55.0% |
| CGACGGCAAGATCGCACCAT+CGG | + | Chr7:18549543-18549562 | MsG0780037047.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 18549506 | 18549898 | 18549506 | ID=MsG0780037047.01;Name=MsG0780037047.01 |
| Chr7 | mRNA | 18549506 | 18549898 | 18549506 | ID=MsG0780037047.01.T01;Parent=MsG0780037047.01;Name=MsG0780037047.01.T01;_AED=0.12;_eAED=0.31;_QI=0|-1|0|1|-1|0|1|0|130 |
| Chr7 | exon | 18549506 | 18549898 | 18549506 | ID=MsG0780037047.01.T01:exon:11800;Parent=MsG0780037047.01.T01 |
| Chr7 | CDS | 18549506 | 18549898 | 18549506 | ID=MsG0780037047.01.T01:cds;Parent=MsG0780037047.01.T01 |
| Gene Sequence |
| Protein sequence |
