AlfalfaGEDB | MsG0380012947.01

M. sativa cultivar ZhongmuNo.1 / MsG0380012947.01

KEGG: K13448
GO: GO:0005509
PFAM: PF13499

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012947.01.T01	XP_003611244.1	87.5	56	7	0	59	114	84	139	6.39E-25	102

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012947.01.T01	Q94AZ4	78.571	56	12	0	59	114	85	140	6.08E-24	93.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012947.01.T01	B7FNA2	87.500	56	7	0	59	114	84	139	3.05e-25	102

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048231.01	MsG0380012947.01	0.801589	8.384190e-49	4.019176e-46
MsG0080048515.01	MsG0380012947.01	0.816470	5.439172e-52	3.857355e-49
MsG0180000241.01	MsG0380012947.01	0.802116	6.536109e-49	3.175464e-46
MsG0180000470.01	MsG0380012947.01	0.808619	2.833580e-50	1.627731e-47
MsG0180002195.01	MsG0380012947.01	0.815265	1.010146e-51	6.931356e-49
MsG0180006054.01	MsG0380012947.01	0.801807	7.564251e-49	3.646275e-46
MsG0380012947.01	MsG0380014727.01	0.810390	1.180548e-50	7.106820e-48
MsG0380012947.01	MsG0380017060.01	0.807954	3.926667e-50	2.216714e-47
MsG0380012947.01	MsG0480018236.01	0.826684	2.369800e-54	2.238080e-51
MsG0380012947.01	MsG0480018318.01	0.825526	4.467114e-54	4.081854e-51
MsG0380012947.01	MsG0480021499.01	0.816789	4.613975e-52	3.300956e-49
MsG0380012947.01	MsG0480021869.01	0.829793	4.220472e-55	4.365139e-52
MsG0380012947.01	MsG0480023251.01	0.824301	8.694052e-54	7.670020e-51
MsG0380012947.01	MsG0580025832.01	0.813912	2.013174e-51	1.331643e-48
MsG0380012947.01	MsG0580028173.01	0.804080	2.564615e-49	1.310244e-46
MsG0380012947.01	MsG0580029376.01	0.803984	2.684666e-49	1.368265e-46
MsG0380012947.01	MsG0680030677.01	0.808378	3.189596e-50	1.820940e-47
MsG0380012947.01	MsG0680030880.01	0.833432	5.351229e-56	6.161578e-53
MsG0380012947.01	MsG0680035444.01	0.806675	7.331538e-50	4.003368e-47
MsG0380012947.01	MsG0780036128.01	0.842932	1.911213e-58	2.942558e-55
MsG0380012947.01	MsG0780036201.01	0.825527	4.464518e-54	4.079592e-51
MsG0380012947.01	MsG0780037047.01	0.841265	5.275566e-58	7.709682e-55
MsG0380012947.01	MsG0780037292.01	0.800124	1.669987e-48	7.714125e-46
MsG0380012947.01	MsG0780039178.01	0.821735	3.447096e-53	2.827894e-50
MsG0380012947.01	MsG0780039573.01	0.812926	3.315341e-51	2.135116e-48
MsG0380012947.01	MsG0780040074.01	0.806965	6.364897e-50	3.501818e-47
MsG0380012947.01	MsG0780040576.01	-0.813012	3.174121e-51	2.048994e-48
MsG0380012947.01	MsG0880042161.01	0.806084	9.767167e-50	5.253696e-47
MsG0380012947.01	MsG0880043157.01	0.812506	4.096222e-51	2.609238e-48
MsG0380012947.01	MsG0880044573.01	0.815472	9.085345e-52	6.270252e-49
MsG0380012947.01	MsG0880046264.01	0.818570	1.829803e-52	1.374611e-49
MsG0280008112.01	MsG0380012947.01	0.810859	9.345997e-51	5.697624e-48
MsG0280008456.01	MsG0380012947.01	0.803838	2.879698e-49	1.462120e-46
MsG0280008894.01	MsG0380012947.01	0.826938	2.060660e-54	1.960569e-51
MsG0280010102.01	MsG0380012947.01	0.804899	1.730506e-49	9.030408e-47
MsG0280010349.01	MsG0380012947.01	0.816535	5.259424e-52	3.736593e-49
MsG0280010517.01	MsG0380012947.01	0.809198	2.129904e-50	1.242608e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012947.01.T01	MTR_5g011850	87.500	56	7	0	59	114	84	139	7.73e-29	102
MsG0380012947.01.T01	MTR_0168s0120	40.789	76	42	1	39	114	33	105	1.77e-15	67.8
MsG0380012947.01.T01	MTR_6g025320	40.789	76	42	1	39	114	33	105	1.77e-15	67.8
MsG0380012947.01.T01	MTR_5g088320	40.789	76	42	1	39	114	69	141	3.72e-15	67.8
MsG0380012947.01.T01	MTR_6g025320	41.333	75	41	1	39	113	69	140	4.05e-15	67.8
MsG0380012947.01.T01	MTR_7g087610	40.000	75	42	1	39	113	69	140	1.19e-14	66.6
MsG0380012947.01.T01	MTR_1g041285	32.927	82	52	1	33	114	63	141	1.34e-12	61.2
MsG0380012947.01.T01	MTR_1g076650	32.099	81	52	1	33	113	63	140	8.01e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012947.01.T01	AT1G12310	78.571	56	12	0	59	114	85	140	6.20e-25	93.2
MsG0380012947.01.T01	AT1G62820	76.786	56	13	0	59	114	85	140	6.54e-25	93.2
MsG0380012947.01.T01	AT3G56800	42.667	75	40	1	39	113	69	140	1.60e-15	68.9
MsG0380012947.01.T01	AT2G27030	42.667	75	40	1	39	113	69	140	1.60e-15	68.9
MsG0380012947.01.T01	AT2G41110	42.667	75	40	1	39	113	69	140	1.60e-15	68.9
MsG0380012947.01.T01	AT3G43810	40.789	76	42	1	39	114	33	105	1.86e-15	67.8
MsG0380012947.01.T01	AT3G43810	40.789	76	42	1	39	114	33	105	1.86e-15	67.8
MsG0380012947.01.T01	AT2G27030	42.105	76	41	1	39	114	69	141	3.09e-15	68.9
MsG0380012947.01.T01	AT5G21274	41.333	75	41	1	39	113	69	140	3.77e-15	67.8
MsG0380012947.01.T01	AT3G43810	41.333	75	41	1	39	113	69	140	4.24e-15	67.8
MsG0380012947.01.T01	AT2G41110	42.105	76	41	1	39	114	81	153	5.56e-15	67.8
MsG0380012947.01.T01	AT3G43810	40.789	76	42	1	39	114	69	141	2.42e-14	67.0
MsG0380012947.01.T01	AT1G66410	38.158	76	44	1	39	114	33	105	4.15e-14	64.3
MsG0380012947.01.T01	AT1G66410	38.158	76	44	1	39	114	33	105	4.15e-14	64.3
MsG0380012947.01.T01	AT5G37780	38.158	76	44	1	39	114	69	141	8.37e-14	64.3
MsG0380012947.01.T01	AT1G66410	38.158	76	44	1	39	114	69	141	8.37e-14	64.3
MsG0380012947.01.T01	AT1G66410	38.158	76	44	1	39	114	79	151	9.69e-14	64.3
MsG0380012947.01.T01	AT5G37780	38.158	76	44	1	39	114	56	128	1.02e-13	63.9
MsG0380012947.01.T01	AT5G37780	38.158	76	44	1	39	114	84	156	1.35e-13	64.3
MsG0380012947.01.T01	AT5G37780	38.158	76	44	1	39	114	95	167	1.87e-13	63.9
MsG0380012947.01.T01	AT3G22930	30.769	91	60	1	24	114	77	164	5.49e-12	60.1
MsG0380012947.01.T01	AT4G14640	29.670	91	61	1	24	114	37	124	3.01e-11	57.4
MsG0380012947.01.T01	AT4G14640	29.670	91	61	1	24	114	55	142	4.87e-11	57.4

Find 30 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATGGATTTGAGTTGGGCT+TGG	0.312614	3:+27456889	None:intergenic
ATTTGAGTTGGGCTTGGGTC+GGG	0.416027	3:+27456895	None:intergenic
CATTGATGAGATTTGAAACT+CGG	0.418570	3:+27457009	None:intergenic
CGGCGACGATGGATTTGAGT+TGG	0.421727	3:+27456883	None:intergenic
GATGGATTTGAGTTGGGCTT+GGG	0.428812	3:+27456890	None:intergenic
GATTTGAGTTGGGCTTGGGT+CGG	0.446248	3:+27456894	None:intergenic
TGAGTTTGATGAATGTATTA+AGG	0.454903	3:-27456747	MsG0380012947.01.T01:CDS
GGCGACGATGGATTTGAGTT+GGG	0.457758	3:+27456884	None:intergenic
TGCGATCTTTCCGTCGTTGT+CGG	0.465353	3:+27456959	None:intergenic
GTTCTCGATAAAGATGCTAC+TGG	0.465422	3:-27456833	MsG0380012947.01.T01:CDS
GAAATCGAAAGGAGAACATT+CGG	0.467430	3:+27456863	None:intergenic
TACCGCCGAGAGAAAGCAAC+AGG	0.474309	3:+27456919	None:intergenic
GGAATCCTGTTGCTTTCTCT+CGG	0.475828	3:-27456924	MsG0380012947.01.T01:CDS
AGGAGAACATTCGGCGACGA+TGG	0.475961	3:+27456872	None:intergenic
TGTATTAAGGAAGTTGATGT+TGG	0.481927	3:-27456734	MsG0380012947.01.T01:CDS
AAGATCGCACCATCGAAGCT+TGG	0.500822	3:-27456945	MsG0380012947.01.T01:CDS
GAAGTTGATGTTGGGTCTGA+TGG	0.505058	3:-27456725	MsG0380012947.01.T01:CDS
AAACAATTGCATTCATCACT+TGG	0.521261	3:+27456665	None:intergenic
ATACATTCATCAAACTCAGC+AGG	0.529884	3:+27456752	None:intergenic
GTATTAAGGAAGTTGATGTT+GGG	0.537176	3:-27456733	MsG0380012947.01.T01:CDS
ATTGATGAGATTTGAAACTC+GGG	0.547054	3:+27457010	None:intergenic
GAACTTTGAGAGAAATCGAA+AGG	0.580421	3:+27456852	None:intergenic
ATGAAGATTCATCGCAGGAA+TGG	0.581704	3:-27456691	MsG0380012947.01.T01:CDS
CCGTTATGAAGATTCATCGC+AGG	0.586768	3:-27456696	MsG0380012947.01.T01:CDS
AAGTTGATGTTGGGTCTGAT+GGG	0.589192	3:-27456724	MsG0380012947.01.T01:CDS
CCTGCGATGAATCTTCATAA+CGG	0.593500	3:+27456696	None:intergenic
CTCTTCGACACCGACAACGA+CGG	0.595446	3:-27456969	MsG0380012947.01.T01:CDS
AACAGGATTCCAAGCTTCGA+TGG	0.609426	3:+27456936	None:intergenic
ATCCTGTTGCTTTCTCTCGG+CGG	0.610054	3:-27456921	MsG0380012947.01.T01:CDS
GATGAGATTTGAAACTCGGG+AGG	0.647816	3:+27457013	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGAGTTTGATGAATGTATTA+AGG	-	Chr3:27456924-27456943	MsG0380012947.01.T01:CDS	25.0%
	AAACAATTGCATTCATCACT+TGG	+	Chr3:27457009-27457028	None:intergenic	30.0%
!	ATTGATGAGATTTGAAACTC+GGG	+	Chr3:27456664-27456683	None:intergenic	30.0%
!	CATTGATGAGATTTGAAACT+CGG	+	Chr3:27456665-27456684	None:intergenic	30.0%
!!	GTATTAAGGAAGTTGATGTT+GGG	-	Chr3:27456938-27456957	MsG0380012947.01.T01:CDS	30.0%
!!	TGTATTAAGGAAGTTGATGT+TGG	-	Chr3:27456937-27456956	MsG0380012947.01.T01:CDS	30.0%
	ATACATTCATCAAACTCAGC+AGG	+	Chr3:27456922-27456941	None:intergenic	35.0%
	GAAATCGAAAGGAGAACATT+CGG	+	Chr3:27456811-27456830	None:intergenic	35.0%
!	GAACTTTGAGAGAAATCGAA+AGG	+	Chr3:27456822-27456841	None:intergenic	35.0%
!!!	CGTCATATTTTGACGAGTAT+TGG	-	Chr3:27456883-27456902	MsG0380012947.01.T01:CDS	35.0%
	ATGAAGATTCATCGCAGGAA+TGG	-	Chr3:27456980-27456999	MsG0380012947.01.T01:CDS	40.0%
	CCTGCGATGAATCTTCATAA+CGG	+	Chr3:27456978-27456997	None:intergenic	40.0%
	GTTCTCGATAAAGATGCTAC+TGG	-	Chr3:27456838-27456857	MsG0380012947.01.T01:CDS	40.0%
!!	AAGTTGATGTTGGGTCTGAT+GGG	-	Chr3:27456947-27456966	MsG0380012947.01.T01:CDS	40.0%
	AACAGGATTCCAAGCTTCGA+TGG	+	Chr3:27456738-27456757	None:intergenic	45.0%
	CCGTTATGAAGATTCATCGC+AGG	-	Chr3:27456975-27456994	MsG0380012947.01.T01:CDS	45.0%
!	GATGAGATTTGAAACTCGGG+AGG	+	Chr3:27456661-27456680	None:intergenic	45.0%
!!	GAAGTTGATGTTGGGTCTGA+TGG	-	Chr3:27456946-27456965	MsG0380012947.01.T01:CDS	45.0%
!!	GATGGATTTGAGTTGGGCTT+GGG	+	Chr3:27456784-27456803	None:intergenic	45.0%
!!	GGAATCCTGTTGCTTTCTCT+CGG	-	Chr3:27456747-27456766	MsG0380012947.01.T01:CDS	45.0%
	AAGATCGCACCATCGAAGCT+TGG	-	Chr3:27456726-27456745	MsG0380012947.01.T01:CDS	50.0%
	TGCGATCTTTCCGTCGTTGT+CGG	+	Chr3:27456715-27456734	None:intergenic	50.0%
!	GGCGACGATGGATTTGAGTT+GGG	+	Chr3:27456790-27456809	None:intergenic	50.0%
!!	ATCCTGTTGCTTTCTCTCGG+CGG	-	Chr3:27456750-27456769	MsG0380012947.01.T01:CDS	50.0%
!!	ATTTGAGTTGGGCTTGGGTC+GGG	+	Chr3:27456779-27456798	None:intergenic	50.0%
!!	CGATGGATTTGAGTTGGGCT+TGG	+	Chr3:27456785-27456804	None:intergenic	50.0%
!!	GATTTGAGTTGGGCTTGGGT+CGG	+	Chr3:27456780-27456799	None:intergenic	50.0%
	AGGAGAACATTCGGCGACGA+TGG	+	Chr3:27456802-27456821	None:intergenic	55.0%
	CTCTTCGACACCGACAACGA+CGG	-	Chr3:27456702-27456721	MsG0380012947.01.T01:CDS	55.0%
	TACCGCCGAGAGAAAGCAAC+AGG	+	Chr3:27456755-27456774	None:intergenic	55.0%
!	CGGCGACGATGGATTTGAGT+TGG	+	Chr3:27456791-27456810	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	27456646	27457047	27456646	ID=MsG0380012947.01;Name=MsG0380012947.01
Chr3	mRNA	27456646	27457047	27456646	ID=MsG0380012947.01.T01;Parent=MsG0380012947.01;Name=MsG0380012947.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|133
Chr3	exon	27456646	27457047	27456646	ID=MsG0380012947.01.T01:exon:22236;Parent=MsG0380012947.01.T01
Chr3	CDS	27456646	27457047	27456646	ID=MsG0380012947.01.T01:cds;Parent=MsG0380012947.01.T01

Gene Sequence

>MsG0380012947.01.T01

ATGTATGATTTACCTCCCGAGTTTCAAATCTCATCAATGAAAGTAGCATATTCACACTCTTCGACACCGACAACGACGGAAAGATCGCACCATCGAAGCTTGGAATCCTGTTGCTTTCTCTCGGCGGTAACCCGACCCAAGCCCAACTCAAATCCATCGTCGCCGAATGTTCTCCTTTCGATTTCTCTCAAAGTTCTCGATAAAGATGCTACTGGATTTGTCTCTGTGAATGAGCTTCGTCATATTTTGACGAGTATTGGTGAGAATCTTGAGCCTGCTGAGTTTGATGAATGTATTAAGGAAGTTGATGTTGGGTCTGATGGGAAGATCCGTTATGAAGATTCATCGCAGGAATGGTTGCCAAGTGATGAATGCAATTGTTTAGTTATTTTCTCTGTTTAA

Protein sequence

>MsG0380012947.01.T01

MYDLPPEFQISSMKVAYSHSSTPTTTERSHHRSLESCCFLSAVTRPKPNSNPSSPNVLLSISLKVLDKDATGFVSVNELRHILTSIGENLEPAEFDECIKEVDVGSDGKIRYEDSSQEWLPSDECNCLVIFSV*