AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039503.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039503.01.T01 MTR_7g062720 64.394 132 47 0 1 132 1 132 2.45e-57 184
MsG0780039503.01.T01 MTR_5g061150 62.205 127 48 0 20 146 10 136 8.11e-48 159
MsG0780039503.01.T01 MTR_4g037355 55.714 140 37 4 151 289 1 116 4.31e-41 141
MsG0780039503.01.T01 MTR_4g112730 69.863 73 20 1 138 210 1 71 1.04e-26 102
MsG0780039503.01.T01 MTR_1g036690 58.537 82 27 3 282 362 5 80 1.14e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 66 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 7:+65002996 MsG0780039503.01.T01:CDS
TTCGGCATTTAAATGAATTT+TGG 0.166319 7:-65003048 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 7:+65003897 MsG0780039503.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 7:+65003889 MsG0780039503.01.T01:CDS
TATGGAAGGGGACGTGGTTT+TGG 0.259444 7:+65003662 MsG0780039503.01.T01:CDS
TGCGTTGCTACATTCACTTC+TGG 0.264941 7:-65003560 None:intergenic
TAGAAGATCCAAAAGATCTT+TGG 0.269782 7:+65003186 MsG0780039503.01.T01:CDS
TATTTCTGGAAATAATTATC+TGG 0.282933 7:+65003010 MsG0780039503.01.T01:CDS
ATGGTTGTATTTGCGTTTAC+AGG 0.299146 7:+65003271 MsG0780039503.01.T01:CDS
TATAGGTGGGGTTCTATTCC+TGG 0.331534 7:-65004051 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 7:-65003866 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 7:+65003949 MsG0780039503.01.T01:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 7:+65003015 MsG0780039503.01.T01:CDS
TGGAAATAATTATCTGGCTT+GGG 0.361577 7:+65003016 MsG0780039503.01.T01:CDS
CGTGGACGTGGTCGTGGTTA+TGG 0.362984 7:+65003644 MsG0780039503.01.T01:CDS
TTTGGGAAAATTTAAAAGAA+AGG 0.385472 7:+65003204 MsG0780039503.01.T01:CDS
AACCATTCGTTACGAGCATT+AGG 0.390171 7:-65003254 None:intergenic
AGAAGATCCAAAAGATCTTT+GGG 0.393236 7:+65003187 MsG0780039503.01.T01:CDS
GAAAATGATGAAGATGATTA+TGG 0.394855 7:+65003941 MsG0780039503.01.T01:CDS
CGTGGTCGTGGCCATGGTTA+TGG 0.406960 7:+65003608 MsG0780039503.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 7:+65003818 MsG0780039503.01.T01:CDS
CGTGGTTATGGAAGGGGACG+TGG 0.429618 7:+65003656 MsG0780039503.01.T01:CDS
GCGTTGCTACATTCACTTCT+GGG 0.431019 7:-65003559 None:intergenic
CCTTCGTCACCACCTTCATA+AGG 0.433246 7:+65003139 MsG0780039503.01.T01:CDS
CAAACTCGTGGTCGTGGTCG+TGG 0.459890 7:+65003596 MsG0780039503.01.T01:CDS
AGAAATCAGCAGCATCTAAG+TGG 0.464577 7:-65003979 None:intergenic
AGTAACAACTGAACAACAAA+AGG 0.468873 7:+65003100 MsG0780039503.01.T01:CDS
AATGGTATCTCCATGCCCTT+CGG 0.470113 7:-65003066 None:intergenic
AATGTTGTAAAGAATGATAT+TGG 0.471241 7:+65003767 MsG0780039503.01.T01:CDS
CGTGGTCGTGGTCGTGGCCA+TGG 0.475975 7:+65003602 MsG0780039503.01.T01:CDS
ATTGACACTGTGTGGAGAAA+AGG 0.483584 7:+65003346 MsG0780039503.01.T01:CDS
GTAGCAACGCATGATCACTA+TGG 0.484635 7:+65003572 MsG0780039503.01.T01:CDS
GCAGCAACAATACCGCGAAA+AGG 0.486558 7:+65003430 MsG0780039503.01.T01:CDS
ACTTCAAAATAAGGATAAAA+GGG 0.487291 7:+65003898 MsG0780039503.01.T01:CDS
GCTACATTCACTTCTGGGAA+TGG 0.488554 7:-65003554 None:intergenic
TACATTAGATGCATGAAAAG+TGG 0.490606 7:-65003402 None:intergenic
CAGCAACAATACCGCGAAAA+GGG 0.491478 7:+65003431 MsG0780039503.01.T01:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 7:+65003056 MsG0780039503.01.T01:CDS
GACGTGGTCGTGGTTATGGA+AGG 0.501950 7:+65003648 MsG0780039503.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 7:+65003825 MsG0780039503.01.T01:CDS
TATGGACAAACTCGTGGTCG+TGG 0.513531 7:+65003590 MsG0780039503.01.T01:CDS
CATGGAGATACCATTAAAGA+AGG 0.521442 7:+65003074 MsG0780039503.01.T01:CDS
CGTGGACGTGGACGTGGTCG+TGG 0.524981 7:+65003638 MsG0780039503.01.T01:CDS
CATTAGAGATGAAAATATCC+AGG 0.532865 7:+65004033 MsG0780039503.01.T01:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 7:+65003050 MsG0780039503.01.T01:CDS
CATGGTTATGGTCGTGGACG+TGG 0.538890 7:+65003620 MsG0780039503.01.T01:CDS
CGTGGCCATGGTTATGGTCG+TGG 0.540024 7:+65003614 MsG0780039503.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.546682 7:+65003632 MsG0780039503.01.T01:CDS
CGTGGTCGTGGTTATGGAAG+GGG 0.551769 7:+65003650 MsG0780039503.01.T01:CDS
TATGGTCGTGGACGTGGACG+TGG 0.556253 7:+65003626 MsG0780039503.01.T01:CDS
TTCCTAATGCTCGTAACGAA+TGG 0.575736 7:+65003252 MsG0780039503.01.T01:CDS
AACTGAACAACAAAAGGCTA+AGG 0.582581 7:+65003106 MsG0780039503.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 7:+65003809 MsG0780039503.01.T01:CDS
ATTCGTTACGAGCATTAGGA+AGG 0.589689 7:-65003250 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 7:-65003139 None:intergenic
ACGTGGTCGTGGTTATGGAA+GGG 0.598966 7:+65003649 MsG0780039503.01.T01:CDS
AGTATTTGCTATCGTTGTGG+AGG 0.599695 7:+65003812 MsG0780039503.01.T01:CDS
AATCACACGACTCGTCCCAC+TGG 0.600994 7:+65003524 MsG0780039503.01.T01:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 7:-65003978 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 7:+65003051 MsG0780039503.01.T01:CDS
CACGTCCACGACCATAACCA+TGG 0.649591 7:-65003619 None:intergenic
GTGGTGACGAAGGAATATCA+TGG 0.663314 7:-65003129 None:intergenic
ACTTCTCTATTGACACTGTG+TGG 0.668278 7:+65003338 MsG0780039503.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 7:-65003540 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 7:-65003539 None:intergenic
GATCACTATGGACAAACTCG+TGG 0.716183 7:+65003584 MsG0780039503.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG + Chr7:65003767-65003786 MsG0780039503.01.T01:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG + Chr7:65003898-65003917 MsG0780039503.01.T01:CDS 20.0%
!! TTTGGGAAAATTTAAAAGAA+AGG + Chr7:65003204-65003223 MsG0780039503.01.T01:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG + Chr7:65003010-65003029 MsG0780039503.01.T01:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG + Chr7:65003050-65003069 MsG0780039503.01.T01:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG + Chr7:65003051-65003070 MsG0780039503.01.T01:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG + Chr7:65003897-65003916 MsG0780039503.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG + Chr7:65003941-65003960 MsG0780039503.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG + Chr7:65003949-65003968 MsG0780039503.01.T01:CDS 25.0%
!! AAATTTTCCCAAAGATCTTT+TGG - Chr7:65003197-65003216 None:intergenic 25.0%
!! ATCATTTTCACATGCAAAAT+GGG - Chr7:65003930-65003949 None:intergenic 25.0%
!! TAAAGATCAACAAGATGTTT+TGG - Chr7:65003869-65003888 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG - Chr7:65003757-65003776 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG - Chr7:65003051-65003070 None:intergenic 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG - Chr7:65003154-65003173 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG - Chr7:65003733-65003752 None:intergenic 25.0%
!!! TAAATGAATTTTGGCATCAA+GGG - Chr7:65003042-65003061 None:intergenic 25.0%
!!! TGTTACTTTTCCTTCTTTAA+TGG - Chr7:65003087-65003106 None:intergenic 25.0%
!!! TTAAATGAATTTTGGCATCA+AGG - Chr7:65003043-65003062 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG + Chr7:65003187-65003206 MsG0780039503.01.T01:CDS 30.0%
AGTAACAACTGAACAACAAA+AGG + Chr7:65003100-65003119 MsG0780039503.01.T01:CDS 30.0%
CATTAGAGATGAAAATATCC+AGG + Chr7:65004033-65004052 MsG0780039503.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG + Chr7:65003889-65003908 MsG0780039503.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG - Chr7:65003405-65003424 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG + Chr7:65003186-65003205 MsG0780039503.01.T01:CDS 30.0%
! AAGATCTTTTGGATCTTCTA+TGG - Chr7:65003186-65003205 None:intergenic 30.0%
! AGATGATCAATCTTTTCATC+AGG - Chr7:65004013-65004032 None:intergenic 30.0%
! CATCATTTTCACATGCAAAA+TGG - Chr7:65003931-65003950 None:intergenic 30.0%
! TGGAAATAATTATCTGGCTT+GGG + Chr7:65003016-65003035 MsG0780039503.01.T01:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG + Chr7:65002996-65003015 MsG0780039503.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG - Chr7:65003151-65003170 None:intergenic 30.0%
!! GGTTTTAATCGTAATCACAA+TGG + Chr7:65003683-65003702 MsG0780039503.01.T01:CDS 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG - Chr7:65003892-65003911 None:intergenic 30.0%
AACTGAACAACAAAAGGCTA+AGG + Chr7:65003106-65003125 MsG0780039503.01.T01:CDS 35.0%
ATGGTTGTATTTGCGTTTAC+AGG + Chr7:65003271-65003290 MsG0780039503.01.T01:CDS 35.0%
CATGGAGATACCATTAAAGA+AGG + Chr7:65003074-65003093 MsG0780039503.01.T01:CDS 35.0%
CTGGAAATAATTATCTGGCT+TGG + Chr7:65003015-65003034 MsG0780039503.01.T01:CDS 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG - Chr7:65002983-65003002 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG + Chr7:65002980-65002999 MsG0780039503.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG - Chr7:65003181-65003200 None:intergenic 35.0%
!!! ACCTTTTAAAACCCTTTTCG+CGG - Chr7:65003445-65003464 None:intergenic 35.0%
AACCATTCGTTACGAGCATT+AGG - Chr7:65003257-65003276 None:intergenic 40.0%
ACTTCTCTATTGACACTGTG+TGG + Chr7:65003338-65003357 MsG0780039503.01.T01:CDS 40.0%
AGAAATCAGCAGCATCTAAG+TGG - Chr7:65003982-65004001 None:intergenic 40.0%
ATTCGTTACGAGCATTAGGA+AGG - Chr7:65003253-65003272 None:intergenic 40.0%
ATTGACACTGTGTGGAGAAA+AGG + Chr7:65003346-65003365 MsG0780039503.01.T01:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG - Chr7:65003981-65004000 None:intergenic 40.0%
TTCCTAATGCTCGTAACGAA+TGG + Chr7:65003252-65003271 MsG0780039503.01.T01:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG - Chr7:65003235-65003254 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG + Chr7:65003812-65003831 MsG0780039503.01.T01:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG + Chr7:65003809-65003828 MsG0780039503.01.T01:CDS 40.0%
!!! ACCGCGAAAAGGGTTTTAAA+AGG + Chr7:65003441-65003460 MsG0780039503.01.T01:CDS 40.0%
AATGGTATCTCCATGCCCTT+CGG - Chr7:65003069-65003088 None:intergenic 45.0%
CAGCAACAATACCGCGAAAA+GGG + Chr7:65003431-65003450 MsG0780039503.01.T01:CDS 45.0%
CATTTAAATGCCGAAGGGCA+TGG + Chr7:65003056-65003075 MsG0780039503.01.T01:CDS 45.0%
GATCACTATGGACAAACTCG+TGG + Chr7:65003584-65003603 MsG0780039503.01.T01:CDS 45.0%
GCGTTGCTACATTCACTTCT+GGG - Chr7:65003562-65003581 None:intergenic 45.0%
GTAGCAACGCATGATCACTA+TGG + Chr7:65003572-65003591 MsG0780039503.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG + Chr7:65003825-65003844 MsG0780039503.01.T01:CDS 45.0%
TGCGTTGCTACATTCACTTC+TGG - Chr7:65003563-65003582 None:intergenic 45.0%
TGCTATCGTTGTGGAGGAAA+AGG + Chr7:65003818-65003837 MsG0780039503.01.T01:CDS 45.0%
! GCTACATTCACTTCTGGGAA+TGG - Chr7:65003557-65003576 None:intergenic 45.0%
! GTGGTGACGAAGGAATATCA+TGG - Chr7:65003132-65003151 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG - Chr7:65003858-65003877 None:intergenic 45.0%
ACGTGGTCGTGGTTATGGAA+GGG + Chr7:65003649-65003668 MsG0780039503.01.T01:CDS 50.0%
CCTTCGTCACCACCTTCATA+AGG + Chr7:65003139-65003158 MsG0780039503.01.T01:CDS 50.0%
GCAGCAACAATACCGCGAAA+AGG + Chr7:65003430-65003449 MsG0780039503.01.T01:CDS 50.0%
TATGGACAAACTCGTGGTCG+TGG + Chr7:65003590-65003609 MsG0780039503.01.T01:CDS 50.0%
! CCTTATGAAGGTGGTGACGA+AGG - Chr7:65003142-65003161 None:intergenic 50.0%
! TATGGAAGGGGACGTGGTTT+TGG + Chr7:65003662-65003681 MsG0780039503.01.T01:CDS 50.0%
AATCACACGACTCGTCCCAC+TGG + Chr7:65003524-65003543 MsG0780039503.01.T01:CDS 55.0%
CACGTCCACGACCATAACCA+TGG - Chr7:65003622-65003641 None:intergenic 55.0%
CATGGTTATGGTCGTGGACG+TGG + Chr7:65003620-65003639 MsG0780039503.01.T01:CDS 55.0%
CGTGGTCGTGGTTATGGAAG+GGG + Chr7:65003650-65003669 MsG0780039503.01.T01:CDS 55.0%
GACGTGGTCGTGGTTATGGA+AGG + Chr7:65003648-65003667 MsG0780039503.01.T01:CDS 55.0%
! GGGAATGGAGCACTACCAGT+GGG - Chr7:65003542-65003561 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG - Chr7:65003543-65003562 None:intergenic 55.0%
CAAACTCGTGGTCGTGGTCG+TGG + Chr7:65003596-65003615 MsG0780039503.01.T01:CDS 60.0%
CGTGGACGTGGTCGTGGTTA+TGG + Chr7:65003644-65003663 MsG0780039503.01.T01:CDS 60.0%
CGTGGCCATGGTTATGGTCG+TGG + Chr7:65003614-65003633 MsG0780039503.01.T01:CDS 60.0%
CGTGGTCGTGGCCATGGTTA+TGG + Chr7:65003608-65003627 MsG0780039503.01.T01:CDS 60.0%
CGTGGTTATGGAAGGGGACG+TGG + Chr7:65003656-65003675 MsG0780039503.01.T01:CDS 60.0%
TATGGTCGTGGACGTGGACG+TGG + Chr7:65003626-65003645 MsG0780039503.01.T01:CDS 60.0%
CGTGGACGTGGACGTGGACG+TGG + Chr7:65003632-65003651 MsG0780039503.01.T01:CDS 70.0%
CGTGGACGTGGACGTGGTCG+TGG + Chr7:65003638-65003657 MsG0780039503.01.T01:CDS 70.0%
CGTGGTCGTGGTCGTGGCCA+TGG + Chr7:65003602-65003621 MsG0780039503.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 65002972 65004060 65002972 ID=MsG0780039503.01;Name=MsG0780039503.01
Chr7 mRNA 65002972 65004060 65002972 ID=MsG0780039503.01.T01;Parent=MsG0780039503.01;Name=MsG0780039503.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|362
Chr7 exon 65002972 65004060 65002972 ID=MsG0780039503.01.T01:exon:10131;Parent=MsG0780039503.01.T01
Chr7 CDS 65002972 65004060 65002972 ID=MsG0780039503.01.T01:cds;Parent=MsG0780039503.01.T01
Gene Sequence

>MsG0780039503.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCCCTTGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACCATTAAAGAAGGAAAAGTAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGAAAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAACGAATGGTTGTATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCAGCTGTATTTAAGATTACTTCTCTATTGACACTGTGTGGAGAAAAGGTGACTGATGCACAAATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCTTGCAGCAACAATACCGCGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGTCTGCTTGTAGCAGAACAAAACAACGAGCTTTTGATGAAAAATCACACGACTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACGCATGATCACTATGGACAAACTCGTGGTCGTGGTCGTGGCCATGGTTATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTATGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAATACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACCCATTTTGCATGTGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGATCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MsG0780039503.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGKVTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWENLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHTTRPTGSAPFPEVNVATHDHYGQTRGRGRGHGYGRGRGRGRGRGYGRGRGFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE*