AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039544.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039544.01.T01 MTR_7g062720 65.909 132 45 0 1 132 1 132 8.25e-60 190
MsG0780039544.01.T01 MTR_5g061150 62.992 127 47 0 20 146 10 136 2.55e-48 160
MsG0780039544.01.T01 MTR_4g037355 55.714 140 37 4 151 289 1 116 4.79e-41 141
MsG0780039544.01.T01 MTR_4g112730 69.863 73 20 1 138 210 1 71 2.12e-25 98.6
MsG0780039544.01.T01 MTR_1g036690 58.537 82 27 3 282 362 5 80 1.08e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 67 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 7:-65619607 MsG0780039544.01.T01:CDS
TTCGGCATTTAAATGAATTT+TGG 0.166319 7:+65619555 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 7:-65618706 MsG0780039544.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 7:-65618714 MsG0780039544.01.T01:CDS
ATGGTTGTATTTACGTTTAC+AGG 0.245586 7:-65619332 MsG0780039544.01.T01:CDS
TATGGAAGGGGACGTGGTTT+TGG 0.259444 7:-65618941 MsG0780039544.01.T01:CDS
TGCGTTGCTACATTCACTTC+TGG 0.264941 7:+65619043 None:intergenic
AAATTGTCCCAAAGATCTTT+TGG 0.267031 7:+65619409 None:intergenic
TATTTCTGGAAATAATTATC+TGG 0.282933 7:-65619593 MsG0780039544.01.T01:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.283634 7:-65619417 MsG0780039544.01.T01:CDS
TATAGGTGGGGTTCTATTCC+TGG 0.331534 7:+65618552 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 7:+65618737 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 7:-65618654 MsG0780039544.01.T01:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 7:-65619588 MsG0780039544.01.T01:CDS
TGGAAATAATTATCTGGCTT+GGG 0.361577 7:-65619587 MsG0780039544.01.T01:CDS
CGTGGACGTGGTCGTGGTTA+TGG 0.362984 7:-65618959 MsG0780039544.01.T01:CDS
GAAAATGATGAAGATGATTA+TGG 0.394855 7:-65618662 MsG0780039544.01.T01:CDS
TTTGGGACAATTTAAAAGAA+AGG 0.395342 7:-65619399 MsG0780039544.01.T01:CDS
AACCATTCATTACGAGCATT+AGG 0.398670 7:+65619349 None:intergenic
AGAAGATCCAAAAGATCTTT+GGG 0.400056 7:-65619416 MsG0780039544.01.T01:CDS
CGTGGTCGTGGCCATGGTTA+TGG 0.406960 7:-65618995 MsG0780039544.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 7:-65618785 MsG0780039544.01.T01:CDS
CGTGGTTATGGAAGGGGACG+TGG 0.429618 7:-65618947 MsG0780039544.01.T01:CDS
GCGTTGCTACATTCACTTCT+GGG 0.431019 7:+65619044 None:intergenic
CCTTCGTCACCACCTTCATA+AGG 0.433246 7:-65619464 MsG0780039544.01.T01:CDS
ATTAACACTGTGTGGAGAAA+AGG 0.440407 7:-65619257 MsG0780039544.01.T01:CDS
CAAAACAACTGAACAACAAA+AGG 0.454927 7:-65619503 MsG0780039544.01.T01:CDS
CAAACTCGTGGTCGTGGTCG+TGG 0.459890 7:-65619007 MsG0780039544.01.T01:CDS
AGAAATCAGCAGCATCTAAG+TGG 0.464577 7:+65618624 None:intergenic
AATGGTATCTCCATGCCCTT+CGG 0.470113 7:+65619537 None:intergenic
AATGTTGTAAAGAATGATAT+TGG 0.471241 7:-65618836 MsG0780039544.01.T01:CDS
CGTGGTCGTGGTCGTGGCCA+TGG 0.475975 7:-65619001 MsG0780039544.01.T01:CDS
GTAGCAACGCATGATCACTA+TGG 0.484635 7:-65619031 MsG0780039544.01.T01:CDS
GCAGCAACAATACCGCGAAA+AGG 0.486558 7:-65619173 MsG0780039544.01.T01:CDS
ACTTCAAAATAAGGATAAAA+GGG 0.487291 7:-65618705 MsG0780039544.01.T01:CDS
GCTACATTCACTTCTGGGAA+TGG 0.488554 7:+65619049 None:intergenic
TACATTAGATGCATGAAAAG+TGG 0.490606 7:+65619201 None:intergenic
CAGCAACAATACCGCGAAAA+GGG 0.491478 7:-65619172 MsG0780039544.01.T01:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 7:-65619547 MsG0780039544.01.T01:CDS
GACGTGGTCGTGGTTATGGA+AGG 0.501950 7:-65618955 MsG0780039544.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 7:-65618778 MsG0780039544.01.T01:CDS
TTCCTAATGCTCGTAATGAA+TGG 0.506622 7:-65619351 MsG0780039544.01.T01:CDS
TATGGACAAACTCGTGGTCG+TGG 0.513531 7:-65619013 MsG0780039544.01.T01:CDS
CATGGAGATACCATTAAAGA+AGG 0.521442 7:-65619529 MsG0780039544.01.T01:CDS
CGTGGACGTGGACGTGGTCG+TGG 0.524981 7:-65618965 MsG0780039544.01.T01:CDS
CATTAGAGATGAAAATATCC+AGG 0.532865 7:-65618570 MsG0780039544.01.T01:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 7:-65619553 MsG0780039544.01.T01:CDS
CATGGTTATGGTCGTGGACG+TGG 0.538890 7:-65618983 MsG0780039544.01.T01:CDS
CGTGGCCATGGTTATGGTCG+TGG 0.540024 7:-65618989 MsG0780039544.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.546682 7:-65618971 MsG0780039544.01.T01:CDS
CGTGGTCGTGGTTATGGAAG+GGG 0.551769 7:-65618953 MsG0780039544.01.T01:CDS
TATGGTCGTGGACGTGGACG+TGG 0.556253 7:-65618977 MsG0780039544.01.T01:CDS
AACTGAACAACAAAAGGCTA+AGG 0.582581 7:-65619497 MsG0780039544.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 7:-65618794 MsG0780039544.01.T01:CDS
ATTCATTACGAGCATTAGGA+AGG 0.589984 7:+65619353 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 7:+65619464 None:intergenic
ACGTGGTCGTGGTTATGGAA+GGG 0.598966 7:-65618954 MsG0780039544.01.T01:CDS
AGTATTTGCTATCGTTGTGG+AGG 0.599695 7:-65618791 MsG0780039544.01.T01:CDS
AATCACACGACTCGTCCCAC+TGG 0.600994 7:-65619079 MsG0780039544.01.T01:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 7:+65618625 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 7:-65619552 MsG0780039544.01.T01:CDS
CACGTCCACGACCATAACCA+TGG 0.649591 7:+65618984 None:intergenic
GTGGTGACGAAGGAATATCA+TGG 0.663314 7:+65619474 None:intergenic
ACTTCTCTATTAACACTGTG+TGG 0.668278 7:-65619265 MsG0780039544.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 7:+65619063 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 7:+65619064 None:intergenic
GATCACTATGGACAAACTCG+TGG 0.716183 7:-65619019 MsG0780039544.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG - Chr7:65619360-65619379 MsG0780039544.01.T01:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG - Chr7:65619491-65619510 MsG0780039544.01.T01:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG - Chr7:65618603-65618622 MsG0780039544.01.T01:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG - Chr7:65618643-65618662 MsG0780039544.01.T01:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG - Chr7:65618644-65618663 MsG0780039544.01.T01:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG - Chr7:65619490-65619509 MsG0780039544.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG - Chr7:65619534-65619553 MsG0780039544.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG - Chr7:65619542-65619561 MsG0780039544.01.T01:CDS 25.0%
!! ATCATTTTCACATGCAAAAT+GGG + Chr7:65619523-65619542 None:intergenic 25.0%
!! TAAAGATCAACAAGATGTTT+TGG + Chr7:65619462-65619481 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG + Chr7:65619350-65619369 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG + Chr7:65618644-65618663 None:intergenic 25.0%
!! TTTGGGACAATTTAAAAGAA+AGG - Chr7:65618797-65618816 MsG0780039544.01.T01:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG + Chr7:65618747-65618766 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG + Chr7:65619326-65619345 None:intergenic 25.0%
!!! TAAATGAATTTTGGCATCAA+GGG + Chr7:65618635-65618654 None:intergenic 25.0%
!!! TGTTTTGTTTCCTTCTTTAA+TGG + Chr7:65618680-65618699 None:intergenic 25.0%
!!! TTAAATGAATTTTGGCATCA+AGG + Chr7:65618636-65618655 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG - Chr7:65618780-65618799 MsG0780039544.01.T01:CDS 30.0%
ATGGTTGTATTTACGTTTAC+AGG - Chr7:65618864-65618883 MsG0780039544.01.T01:CDS 30.0%
CAAAACAACTGAACAACAAA+AGG - Chr7:65618693-65618712 MsG0780039544.01.T01:CDS 30.0%
CATTAGAGATGAAAATATCC+AGG - Chr7:65619626-65619645 MsG0780039544.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG - Chr7:65619482-65619501 MsG0780039544.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG + Chr7:65618998-65619017 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG - Chr7:65618779-65618798 MsG0780039544.01.T01:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG + Chr7:65618790-65618809 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG + Chr7:65618779-65618798 None:intergenic 30.0%
! AGATGATCAATCTTTTCATC+AGG + Chr7:65619606-65619625 None:intergenic 30.0%
! CATCATTTTCACATGCAAAA+TGG + Chr7:65619524-65619543 None:intergenic 30.0%
! TGGAAATAATTATCTGGCTT+GGG - Chr7:65618609-65618628 MsG0780039544.01.T01:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG - Chr7:65618589-65618608 MsG0780039544.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG + Chr7:65618744-65618763 None:intergenic 30.0%
!! GGTTTTAATCGTAATCACAA+TGG - Chr7:65619276-65619295 MsG0780039544.01.T01:CDS 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG + Chr7:65619485-65619504 None:intergenic 30.0%
AACCATTCATTACGAGCATT+AGG + Chr7:65618850-65618869 None:intergenic 35.0%
AACTGAACAACAAAAGGCTA+AGG - Chr7:65618699-65618718 MsG0780039544.01.T01:CDS 35.0%
ACTTCTCTATTAACACTGTG+TGG - Chr7:65618931-65618950 MsG0780039544.01.T01:CDS 35.0%
ATTAACACTGTGTGGAGAAA+AGG - Chr7:65618939-65618958 MsG0780039544.01.T01:CDS 35.0%
ATTCATTACGAGCATTAGGA+AGG + Chr7:65618846-65618865 None:intergenic 35.0%
CATGGAGATACCATTAAAGA+AGG - Chr7:65618667-65618686 MsG0780039544.01.T01:CDS 35.0%
CTGGAAATAATTATCTGGCT+TGG - Chr7:65618608-65618627 MsG0780039544.01.T01:CDS 35.0%
TTCCTAATGCTCGTAATGAA+TGG - Chr7:65618845-65618864 MsG0780039544.01.T01:CDS 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG + Chr7:65618576-65618595 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG - Chr7:65618573-65618592 MsG0780039544.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG + Chr7:65618774-65618793 None:intergenic 35.0%
!!! ACCTTTTAAAACCCTTTTCG+CGG + Chr7:65619038-65619057 None:intergenic 35.0%
AGAAATCAGCAGCATCTAAG+TGG + Chr7:65619575-65619594 None:intergenic 40.0%
GAAATCAGCAGCATCTAAGT+GGG + Chr7:65619574-65619593 None:intergenic 40.0%
! GAAGGATGACAGTTTTCTGA+TGG + Chr7:65618828-65618847 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG - Chr7:65619405-65619424 MsG0780039544.01.T01:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG - Chr7:65619402-65619421 MsG0780039544.01.T01:CDS 40.0%
!!! ACCGCGAAAAGGGTTTTAAA+AGG - Chr7:65619034-65619053 MsG0780039544.01.T01:CDS 40.0%
AATGGTATCTCCATGCCCTT+CGG + Chr7:65618662-65618681 None:intergenic 45.0%
CAGCAACAATACCGCGAAAA+GGG - Chr7:65619024-65619043 MsG0780039544.01.T01:CDS 45.0%
CATTTAAATGCCGAAGGGCA+TGG - Chr7:65618649-65618668 MsG0780039544.01.T01:CDS 45.0%
GATCACTATGGACAAACTCG+TGG - Chr7:65619177-65619196 MsG0780039544.01.T01:CDS 45.0%
GCGTTGCTACATTCACTTCT+GGG + Chr7:65619155-65619174 None:intergenic 45.0%
GTAGCAACGCATGATCACTA+TGG - Chr7:65619165-65619184 MsG0780039544.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG - Chr7:65619418-65619437 MsG0780039544.01.T01:CDS 45.0%
TGCGTTGCTACATTCACTTC+TGG + Chr7:65619156-65619175 None:intergenic 45.0%
TGCTATCGTTGTGGAGGAAA+AGG - Chr7:65619411-65619430 MsG0780039544.01.T01:CDS 45.0%
! GCTACATTCACTTCTGGGAA+TGG + Chr7:65619150-65619169 None:intergenic 45.0%
! GTGGTGACGAAGGAATATCA+TGG + Chr7:65618725-65618744 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG + Chr7:65619451-65619470 None:intergenic 45.0%
ACGTGGTCGTGGTTATGGAA+GGG - Chr7:65619242-65619261 MsG0780039544.01.T01:CDS 50.0%
CCTTCGTCACCACCTTCATA+AGG - Chr7:65618732-65618751 MsG0780039544.01.T01:CDS 50.0%
GCAGCAACAATACCGCGAAA+AGG - Chr7:65619023-65619042 MsG0780039544.01.T01:CDS 50.0%
TATGGACAAACTCGTGGTCG+TGG - Chr7:65619183-65619202 MsG0780039544.01.T01:CDS 50.0%
! CCTTATGAAGGTGGTGACGA+AGG + Chr7:65618735-65618754 None:intergenic 50.0%
! TATGGAAGGGGACGTGGTTT+TGG - Chr7:65619255-65619274 MsG0780039544.01.T01:CDS 50.0%
AATCACACGACTCGTCCCAC+TGG - Chr7:65619117-65619136 MsG0780039544.01.T01:CDS 55.0%
CACGTCCACGACCATAACCA+TGG + Chr7:65619215-65619234 None:intergenic 55.0%
CATGGTTATGGTCGTGGACG+TGG - Chr7:65619213-65619232 MsG0780039544.01.T01:CDS 55.0%
CGTGGTCGTGGTTATGGAAG+GGG - Chr7:65619243-65619262 MsG0780039544.01.T01:CDS 55.0%
GACGTGGTCGTGGTTATGGA+AGG - Chr7:65619241-65619260 MsG0780039544.01.T01:CDS 55.0%
! GGGAATGGAGCACTACCAGT+GGG + Chr7:65619135-65619154 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG + Chr7:65619136-65619155 None:intergenic 55.0%
CAAACTCGTGGTCGTGGTCG+TGG - Chr7:65619189-65619208 MsG0780039544.01.T01:CDS 60.0%
CGTGGACGTGGTCGTGGTTA+TGG - Chr7:65619237-65619256 MsG0780039544.01.T01:CDS 60.0%
CGTGGCCATGGTTATGGTCG+TGG - Chr7:65619207-65619226 MsG0780039544.01.T01:CDS 60.0%
CGTGGTCGTGGCCATGGTTA+TGG - Chr7:65619201-65619220 MsG0780039544.01.T01:CDS 60.0%
CGTGGTTATGGAAGGGGACG+TGG - Chr7:65619249-65619268 MsG0780039544.01.T01:CDS 60.0%
TATGGTCGTGGACGTGGACG+TGG - Chr7:65619219-65619238 MsG0780039544.01.T01:CDS 60.0%
CGTGGACGTGGACGTGGACG+TGG - Chr7:65619225-65619244 MsG0780039544.01.T01:CDS 70.0%
CGTGGACGTGGACGTGGTCG+TGG - Chr7:65619231-65619250 MsG0780039544.01.T01:CDS 70.0%
CGTGGTCGTGGTCGTGGCCA+TGG - Chr7:65619195-65619214 MsG0780039544.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 65618565 65619653 65618565 ID=MsG0780039544.01;Name=MsG0780039544.01
Chr7 mRNA 65618565 65619653 65618565 ID=MsG0780039544.01.T01;Parent=MsG0780039544.01;Name=MsG0780039544.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|362
Chr7 exon 65618565 65619653 65618565 ID=MsG0780039544.01.T01:exon:10399;Parent=MsG0780039544.01.T01
Chr7 CDS 65618565 65619653 65618565 ID=MsG0780039544.01.T01:cds;Parent=MsG0780039544.01.T01
Gene Sequence

>MsG0780039544.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCCCTTGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACCATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTGTATTTACGTTTACAGGACTTTAAGTCTGTAATCGAATATAATTCAGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGGTGACTGATGCACAAATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCTTGCAGCAACAATACCGCGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGTCTGCTTGTAGCAGAACAAAATAACGAGCTTTTGATGAAAAATCACACGACTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACGCATGATCACTATGGACAAACTCGTGGTCGTGGTCGTGGCCATGGTTATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTATGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAATACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACCCATTTTGCATGTGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGATCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MsG0780039544.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHTTRPTGSAPFPEVNVATHDHYGQTRGRGRGHGYGRGRGRGRGRGYGRGRGFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE*