AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024918.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024918.01.T01 MTR_7g062720 65.909 132 45 0 1 132 1 132 8.67e-60 190
MsG0580024918.01.T01 MTR_5g061150 62.992 127 47 0 20 146 10 136 2.58e-48 160
MsG0580024918.01.T01 MTR_4g037355 57.971 138 35 5 151 287 1 116 2.31e-41 142
MsG0580024918.01.T01 MTR_4g112730 71.831 71 18 1 138 208 1 69 1.12e-26 102
MsG0580024918.01.T01 MTR_1g036690 58.537 82 27 3 280 360 5 80 1.12e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 5:-11656204 MsG0580024918.01.T01:CDS
TTCGGCATTTAAATGAATTT+TGG 0.166319 5:+11656152 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 5:-11655309 MsG0580024918.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 5:-11655317 MsG0580024918.01.T01:CDS
ATGGTTATATTTGCGTTTAC+AGG 0.259438 5:-11655929 MsG0580024918.01.T01:CDS
AAATTGTCCCAAAGATCTTT+TGG 0.267031 5:+11656006 None:intergenic
TATAGGTGGGGTTCTATTCT+TGG 0.275831 5:+11655155 None:intergenic
TATTTCTGGAAATAATTATC+TGG 0.282933 5:-11656190 MsG0580024918.01.T01:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.283634 5:-11656014 MsG0580024918.01.T01:CDS
TGTGTTGCTACATTCACTTC+TGG 0.294995 5:+11655640 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 5:+11655340 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 5:-11655257 MsG0580024918.01.T01:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 5:-11656185 MsG0580024918.01.T01:CDS
TATGGAAGGAGACGTGGTTT+TGG 0.347848 5:-11655544 MsG0580024918.01.T01:CDS
TGGAAATAATTATCTGGCTT+GGG 0.353242 5:-11656184 MsG0580024918.01.T01:CDS
CGTGGACGTGGTCATGGTTA+TGG 0.384243 5:-11655562 MsG0580024918.01.T01:CDS
AACCATTCATTACGAGCATT+AGG 0.386252 5:+11655946 None:intergenic
GAAAATGATGAAGATGATTA+TGG 0.391347 5:-11655265 MsG0580024918.01.T01:CDS
TTTGGGACAATTTAAAAGAA+AGG 0.395342 5:-11655996 MsG0580024918.01.T01:CDS
AGAAGATCCAAAAGATCTTT+GGG 0.400056 5:-11656013 MsG0580024918.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 5:-11655388 MsG0580024918.01.T01:CDS
GTGTTGCTACATTCACTTCT+GGG 0.423307 5:+11655641 None:intergenic
CCTTCGTCACCACCTTCATA+AGG 0.433246 5:-11656061 MsG0580024918.01.T01:CDS
ATTAACACTGTGTGGAGAAA+AGG 0.440407 5:-11655854 MsG0580024918.01.T01:CDS
CAAAATCGTGGTCGTGGCCG+TGG 0.442209 5:-11655604 MsG0580024918.01.T01:CDS
CGTGGTCGTGGCCGTGGTCA+TGG 0.454221 5:-11655598 MsG0580024918.01.T01:CDS
CAAAACAACTGAACAACAAA+AGG 0.454927 5:-11656100 MsG0580024918.01.T01:CDS
AATTGTATCTCCATGCCCTT+CGG 0.460742 5:+11656134 None:intergenic
AGAAATCAGCAGCATCTAAG+TGG 0.464577 5:+11655227 None:intergenic
TTTCACGGTATTGTTGCTGC+AGG 0.465441 5:+11655773 None:intergenic
AATGTTGTAAAGAATGATAT+TGG 0.471241 5:-11655439 MsG0580024918.01.T01:CDS
GCAGCAACAATACCGTGAAA+AGG 0.474876 5:-11655770 MsG0580024918.01.T01:CDS
AATCACTCGGCTCGTCCCAC+TGG 0.475930 5:-11655676 MsG0580024918.01.T01:CDS
GCTACATTCACTTCTGGGAA+TGG 0.483138 5:+11655646 None:intergenic
ACTTCAAAATAAGGATAAAA+GGG 0.487291 5:-11655308 MsG0580024918.01.T01:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 5:-11656144 MsG0580024918.01.T01:CDS
CAGCAACAATACCGTGAAAA+GGG 0.498612 5:-11655769 MsG0580024918.01.T01:CDS
GTAGCAACACATGATCACTA+TGG 0.504040 5:-11655628 MsG0580024918.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 5:-11655381 MsG0580024918.01.T01:CDS
CGTGGCCGTGGTCATGGTCG+TGG 0.506333 5:-11655592 MsG0580024918.01.T01:CDS
TTCCTAATGCTCGTAATGAA+TGG 0.506622 5:-11655948 MsG0580024918.01.T01:CDS
TACATTAGATGCATGAAAAG+TGG 0.516304 5:+11655798 None:intergenic
CATGGTCGTGGACGTGGACG+TGG 0.524744 5:-11655580 MsG0580024918.01.T01:CDS
CATGGAGATACAATTAAAGA+AGG 0.533844 5:-11656126 MsG0580024918.01.T01:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 5:-11656150 MsG0580024918.01.T01:CDS
CGTGGTCATGGTCGTGGACG+TGG 0.540833 5:-11655586 MsG0580024918.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.546682 5:-11655574 MsG0580024918.01.T01:CDS
CGTGGACGTGGACGTGGTCA+TGG 0.554275 5:-11655568 MsG0580024918.01.T01:CDS
CTTAATATCATGCCTGCTTG+TGG 0.567363 5:-11655728 MsG0580024918.01.T01:CDS
TATGGACAAAATCGTGGTCG+TGG 0.573891 5:-11655610 MsG0580024918.01.T01:CDS
GTCTGTAATCGAATATAACT+CGG 0.574961 5:-11655899 MsG0580024918.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 5:-11655397 MsG0580024918.01.T01:CDS
ATTCATTACGAGCATTAGGA+AGG 0.589984 5:+11655950 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 5:+11656061 None:intergenic
AGTATTTGCTATCGTTGTGG+AGG 0.599695 5:-11655394 MsG0580024918.01.T01:CDS
CATGGTTATGGAAGGAGACG+TGG 0.612985 5:-11655550 MsG0580024918.01.T01:CDS
GACGTGGTCATGGTTATGGA+AGG 0.616713 5:-11655558 MsG0580024918.01.T01:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 5:+11655228 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 5:-11656149 MsG0580024918.01.T01:CDS
CACGTCCACGACCATGACCA+CGG 0.661673 5:+11655587 None:intergenic
GTGGTGACGAAGGAATATCA+TGG 0.663314 5:+11656071 None:intergenic
ACTTCTCTATTAACACTGTG+TGG 0.668278 5:-11655862 MsG0580024918.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 5:+11655660 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 5:+11655661 None:intergenic
GATCACTATGGACAAAATCG+TGG 0.728183 5:-11655616 MsG0580024918.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG - Chr5:11655957-11655976 MsG0580024918.01.T01:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG - Chr5:11656088-11656107 MsG0580024918.01.T01:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG - Chr5:11655206-11655225 MsG0580024918.01.T01:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG - Chr5:11655246-11655265 MsG0580024918.01.T01:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG - Chr5:11655247-11655266 MsG0580024918.01.T01:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG - Chr5:11656087-11656106 MsG0580024918.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG - Chr5:11656131-11656150 MsG0580024918.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG - Chr5:11656139-11656158 MsG0580024918.01.T01:CDS 25.0%
!! TAAAGATCAACAAGATGTTT+TGG + Chr5:11656059-11656078 None:intergenic 25.0%
!! TATTTTCATCTCTAATGAGA+TGG + Chr5:11656220-11656239 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG + Chr5:11655947-11655966 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG + Chr5:11655247-11655266 None:intergenic 25.0%
!! TTTGGGACAATTTAAAAGAA+AGG - Chr5:11655400-11655419 MsG0580024918.01.T01:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG + Chr5:11655350-11655369 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG + Chr5:11655923-11655942 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG - Chr5:11655383-11655402 MsG0580024918.01.T01:CDS 30.0%
ATGGTTATATTTGCGTTTAC+AGG - Chr5:11655467-11655486 MsG0580024918.01.T01:CDS 30.0%
CAAAACAACTGAACAACAAA+AGG - Chr5:11655296-11655315 MsG0580024918.01.T01:CDS 30.0%
CATGGAGATACAATTAAAGA+AGG - Chr5:11655270-11655289 MsG0580024918.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG - Chr5:11656079-11656098 MsG0580024918.01.T01:CDS 30.0%
GTCTGTAATCGAATATAACT+CGG - Chr5:11655497-11655516 MsG0580024918.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG + Chr5:11655601-11655620 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG - Chr5:11655382-11655401 MsG0580024918.01.T01:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG + Chr5:11655393-11655412 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG + Chr5:11655382-11655401 None:intergenic 30.0%
! GCTTTTGATGAAAAATCACT+CGG - Chr5:11655707-11655726 MsG0580024918.01.T01:CDS 30.0%
! TGGAAATAATTATCTGGCTT+GGG - Chr5:11655212-11655231 MsG0580024918.01.T01:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG - Chr5:11655192-11655211 MsG0580024918.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG + Chr5:11655347-11655366 None:intergenic 30.0%
!!! ACCTTTTAAAACCCTTTTCA+CGG + Chr5:11655641-11655660 None:intergenic 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG + Chr5:11656082-11656101 None:intergenic 30.0%
AACCATTCATTACGAGCATT+AGG + Chr5:11655453-11655472 None:intergenic 35.0%
ACTTCTCTATTAACACTGTG+TGG - Chr5:11655534-11655553 MsG0580024918.01.T01:CDS 35.0%
ATTAACACTGTGTGGAGAAA+AGG - Chr5:11655542-11655561 MsG0580024918.01.T01:CDS 35.0%
ATTCATTACGAGCATTAGGA+AGG + Chr5:11655449-11655468 None:intergenic 35.0%
CTGGAAATAATTATCTGGCT+TGG - Chr5:11655211-11655230 MsG0580024918.01.T01:CDS 35.0%
TTCCTAATGCTCGTAATGAA+TGG - Chr5:11655448-11655467 MsG0580024918.01.T01:CDS 35.0%
! AGATGGTCAATCTTTTCATC+AGG + Chr5:11656203-11656222 None:intergenic 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG + Chr5:11655179-11655198 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG - Chr5:11655176-11655195 MsG0580024918.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG + Chr5:11655377-11655396 None:intergenic 35.0%
!!! ACCGTGAAAAGGGTTTTAAA+AGG - Chr5:11655637-11655656 MsG0580024918.01.T01:CDS 35.0%
AATTGTATCTCCATGCCCTT+CGG + Chr5:11655265-11655284 None:intergenic 40.0%
AGAAATCAGCAGCATCTAAG+TGG + Chr5:11656172-11656191 None:intergenic 40.0%
CAGCAACAATACCGTGAAAA+GGG - Chr5:11655627-11655646 MsG0580024918.01.T01:CDS 40.0%
CTTAATATCATGCCTGCTTG+TGG - Chr5:11655668-11655687 MsG0580024918.01.T01:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG + Chr5:11656171-11656190 None:intergenic 40.0%
GATCACTATGGACAAAATCG+TGG - Chr5:11655780-11655799 MsG0580024918.01.T01:CDS 40.0%
GTAGCAACACATGATCACTA+TGG - Chr5:11655768-11655787 MsG0580024918.01.T01:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG + Chr5:11655431-11655450 None:intergenic 40.0%
! GTGTTGCTACATTCACTTCT+GGG + Chr5:11655758-11655777 None:intergenic 40.0%
! TATTTTGTTCTGCCACAAGC+AGG + Chr5:11655683-11655702 None:intergenic 40.0%
! TGTGTTGCTACATTCACTTC+TGG + Chr5:11655759-11655778 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG - Chr5:11656002-11656021 MsG0580024918.01.T01:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG - Chr5:11655999-11656018 MsG0580024918.01.T01:CDS 40.0%
CATTTAAATGCCGAAGGGCA+TGG - Chr5:11655252-11655271 MsG0580024918.01.T01:CDS 45.0%
GCAGCAACAATACCGTGAAA+AGG - Chr5:11655626-11655645 MsG0580024918.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG - Chr5:11656015-11656034 MsG0580024918.01.T01:CDS 45.0%
TATGGACAAAATCGTGGTCG+TGG - Chr5:11655786-11655805 MsG0580024918.01.T01:CDS 45.0%
TGCTATCGTTGTGGAGGAAA+AGG - Chr5:11656008-11656027 MsG0580024918.01.T01:CDS 45.0%
TTTCACGGTATTGTTGCTGC+AGG + Chr5:11655626-11655645 None:intergenic 45.0%
! GCTACATTCACTTCTGGGAA+TGG + Chr5:11655753-11655772 None:intergenic 45.0%
! GTGGTGACGAAGGAATATCA+TGG + Chr5:11655328-11655347 None:intergenic 45.0%
! TATGGAAGGAGACGTGGTTT+TGG - Chr5:11655852-11655871 MsG0580024918.01.T01:CDS 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG + Chr5:11656048-11656067 None:intergenic 45.0%
CATGGTTATGGAAGGAGACG+TGG - Chr5:11655846-11655865 MsG0580024918.01.T01:CDS 50.0%
CCTTCGTCACCACCTTCATA+AGG - Chr5:11655335-11655354 MsG0580024918.01.T01:CDS 50.0%
GACGTGGTCATGGTTATGGA+AGG - Chr5:11655838-11655857 MsG0580024918.01.T01:CDS 50.0%
! CCTTATGAAGGTGGTGACGA+AGG + Chr5:11655338-11655357 None:intergenic 50.0%
CGTGGACGTGGTCATGGTTA+TGG - Chr5:11655834-11655853 MsG0580024918.01.T01:CDS 55.0%
! GGGAATGGAGCACTACCAGT+GGG + Chr5:11655738-11655757 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG + Chr5:11655739-11655758 None:intergenic 55.0%
CAAAATCGTGGTCGTGGCCG+TGG - Chr5:11655792-11655811 MsG0580024918.01.T01:CDS 60.0%
CACGTCCACGACCATGACCA+CGG + Chr5:11655812-11655831 None:intergenic 60.0%
! AATCACTCGGCTCGTCCCAC+TGG - Chr5:11655720-11655739 MsG0580024918.01.T01:CDS 60.0%
CATGGTCGTGGACGTGGACG+TGG - Chr5:11655816-11655835 MsG0580024918.01.T01:CDS 65.0%
CGTGGACGTGGACGTGGTCA+TGG - Chr5:11655828-11655847 MsG0580024918.01.T01:CDS 65.0%
CGTGGTCATGGTCGTGGACG+TGG - Chr5:11655810-11655829 MsG0580024918.01.T01:CDS 65.0%
CGTGGACGTGGACGTGGACG+TGG - Chr5:11655822-11655841 MsG0580024918.01.T01:CDS 70.0%
CGTGGCCGTGGTCATGGTCG+TGG - Chr5:11655804-11655823 MsG0580024918.01.T01:CDS 70.0%
CGTGGTCGTGGCCGTGGTCA+TGG - Chr5:11655798-11655817 MsG0580024918.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 11655168 11656250 11655168 ID=MsG0580024918.01;Name=MsG0580024918.01
Chr5 mRNA 11655168 11656250 11655168 ID=MsG0580024918.01.T01;Parent=MsG0580024918.01;Name=MsG0580024918.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|360
Chr5 exon 11655168 11656250 11655168 ID=MsG0580024918.01.T01:exon:19830;Parent=MsG0580024918.01.T01
Chr5 CDS 11655168 11656250 11655168 ID=MsG0580024918.01.T01:cds;Parent=MsG0580024918.01.T01
Gene Sequence

>MsG0580024918.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCTCTAGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACAATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAAGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTATATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGGTGACTGATGCGCAGATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCCTGCAGCAACAATACCGTGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGCCTGCTTGTGGCAGAACAAAATAACGAGCTTTTGATGAAAAATCACTCGGCTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACACATGATCACTATGGACAAAATCGTGGTCGTGGCCGTGGTCATGGTCGTGGACGTGGACGTGGACGTGGTCATGGTTATGGAAGGAGACGTGGTTTTGGTTTTAATCGTAATCACAATAGAGATTACAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACACACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGACCATCTCATTAGAGATGAAAATATCCAAGAATAG

Protein sequence

>MsG0580024918.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHSARPTGSAPFPEVNVATHDHYGQNRGRGRGHGRGRGRGRGHGYGRRRGFGFNRNHNRDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE*