AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038597.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038597.01.T01 MTR_7g062720 65.152 132 46 0 1 132 1 132 2.65e-58 186
MsG0780038597.01.T01 MTR_5g061150 62.205 127 48 0 20 146 10 136 7.64e-47 156
MsG0780038597.01.T01 MTR_4g037355 57.246 138 36 5 151 287 1 116 1.71e-40 139
MsG0780038597.01.T01 MTR_4g112730 68.493 73 21 1 138 210 1 71 1.30e-24 96.7
MsG0780038597.01.T01 MTR_1g036690 58.537 82 27 3 280 360 5 80 1.07e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 7:+49536115 MsG0780038597.01.T01:CDS
TATAGGTGGGGTTCTATTTC+TGG 0.146869 7:-49537164 None:intergenic
TTCGGCATTTAAATGAATTT+TGG 0.166319 7:-49536167 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 7:+49537010 MsG0780038597.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 7:+49537002 MsG0780038597.01.T01:CDS
ATGGTTATATTTGCGTTTAC+AGG 0.259438 7:+49536390 MsG0780038597.01.T01:CDS
TATGGAAGGGGACGTGGTTT+TGG 0.259444 7:+49536775 MsG0780038597.01.T01:CDS
AAATTGTCCCAAAGATCTTT+TGG 0.267031 7:-49536313 None:intergenic
TATTTCTGGAAATAATTATC+TGG 0.282933 7:+49536129 MsG0780038597.01.T01:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.283634 7:+49536305 MsG0780038597.01.T01:CDS
TGTGTTGCTACATTCACTTC+TGG 0.294995 7:-49536679 None:intergenic
TAAAGATCAGCAAGATGTTT+TGG 0.328294 7:-49536979 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 7:+49537062 MsG0780038597.01.T01:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 7:+49536134 MsG0780038597.01.T01:CDS
TGGAAATAATTATCTGGCTT+GGG 0.353242 7:+49536135 MsG0780038597.01.T01:CDS
CGTGGACGTGGTCGTGGTTA+TGG 0.362984 7:+49536757 MsG0780038597.01.T01:CDS
AACCATTCATTACGAGCATT+AGG 0.386252 7:-49536373 None:intergenic
GAAAATGATGAAGATGATTA+TGG 0.391347 7:+49537054 MsG0780038597.01.T01:CDS
TTTGGGACAATTTAAAAGAA+AGG 0.395342 7:+49536323 MsG0780038597.01.T01:CDS
AGAAGATCCAAAAGATCTTT+GGG 0.400056 7:+49536306 MsG0780038597.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 7:+49536931 MsG0780038597.01.T01:CDS
GTGTTGCTACATTCACTTCT+GGG 0.423307 7:-49536678 None:intergenic
CCTTTGTCACCACCTTCATA+AGG 0.426832 7:+49536258 MsG0780038597.01.T01:CDS
CGTGGTTATGGAAGGGGACG+TGG 0.429618 7:+49536769 MsG0780038597.01.T01:CDS
ATTAACACTGTGTGGAGAAA+AGG 0.440407 7:+49536465 MsG0780038597.01.T01:CDS
CAAAATCGTGGTCGTGGCCG+TGG 0.442209 7:+49536715 MsG0780038597.01.T01:CDS
CGTGGTCGTGGCCGTGGTCA+TGG 0.454221 7:+49536721 MsG0780038597.01.T01:CDS
CAAAACAACTGAACAACAAA+AGG 0.454927 7:+49536219 MsG0780038597.01.T01:CDS
AATTGTATCTCCATGCCCTT+CGG 0.460742 7:-49536185 None:intergenic
AGAAATCAGCAGCATCTAAG+TGG 0.464577 7:-49537092 None:intergenic
TTTCACGGTATTGTTGCTGC+AGG 0.465441 7:-49536546 None:intergenic
AATGTTGTAAAGAATGATAT+TGG 0.471241 7:+49536880 MsG0780038597.01.T01:CDS
GCAGCAACAATACCGTGAAA+AGG 0.474876 7:+49536549 MsG0780038597.01.T01:CDS
AATCACTCGGCTCGTCCCAC+TGG 0.475930 7:+49536643 MsG0780038597.01.T01:CDS
GCTACATTCACTTCTGGGAA+TGG 0.483138 7:-49536673 None:intergenic
ACTTCAAAATAAGGATAAAA+GGG 0.487291 7:+49537011 MsG0780038597.01.T01:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 7:+49536175 MsG0780038597.01.T01:CDS
CAGCAACAATACCGTGAAAA+GGG 0.498612 7:+49536550 MsG0780038597.01.T01:CDS
GACGTGGTCGTGGTTATGGA+AGG 0.501950 7:+49536761 MsG0780038597.01.T01:CDS
GTAGCAACACATGATCACTA+TGG 0.504040 7:+49536691 MsG0780038597.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 7:+49536938 MsG0780038597.01.T01:CDS
CGTGGCCGTGGTCATGGTCG+TGG 0.506333 7:+49536727 MsG0780038597.01.T01:CDS
TTCCTAATGCTCGTAATGAA+TGG 0.506622 7:+49536371 MsG0780038597.01.T01:CDS
TACATTAGATGCATGAAAAG+TGG 0.516304 7:-49536521 None:intergenic
CATGGTCGTGGACGTGGACG+TGG 0.524744 7:+49536739 MsG0780038597.01.T01:CDS
CGTGGACGTGGACGTGGTCG+TGG 0.524981 7:+49536751 MsG0780038597.01.T01:CDS
CATGGAGATACAATTAAAGA+AGG 0.533844 7:+49536193 MsG0780038597.01.T01:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 7:+49536169 MsG0780038597.01.T01:CDS
CGTGGTCATGGTCGTGGACG+TGG 0.540833 7:+49536733 MsG0780038597.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.546682 7:+49536745 MsG0780038597.01.T01:CDS
CCTTATGAAGGTGGTGACAA+AGG 0.550160 7:-49536258 None:intergenic
CGTGGTCGTGGTTATGGAAG+GGG 0.551769 7:+49536763 MsG0780038597.01.T01:CDS
CTTAATATCATGCCTGCTTG+TGG 0.567363 7:+49536591 MsG0780038597.01.T01:CDS
TATGGACAAAATCGTGGTCG+TGG 0.573891 7:+49536709 MsG0780038597.01.T01:CDS
GTCTGTAATCGAATATAACT+CGG 0.574961 7:+49536420 MsG0780038597.01.T01:CDS
AACTGAACAACAAAAGGCTA+AGG 0.582581 7:+49536225 MsG0780038597.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 7:+49536922 MsG0780038597.01.T01:CDS
ATTCATTACGAGCATTAGGA+AGG 0.589984 7:-49536369 None:intergenic
ACGTGGTCGTGGTTATGGAA+GGG 0.598966 7:+49536762 MsG0780038597.01.T01:CDS
AGTATTTGCTATCGTTGTGG+AGG 0.599695 7:+49536925 MsG0780038597.01.T01:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 7:-49537091 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 7:+49536170 MsG0780038597.01.T01:CDS
GTGGTGACAAAGGAATATCA+TGG 0.639743 7:-49536248 None:intergenic
CACGTCCACGACCATGACCA+CGG 0.661673 7:-49536732 None:intergenic
ACTTCTCTATTAACACTGTG+TGG 0.668278 7:+49536457 MsG0780038597.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 7:-49536659 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 7:-49536658 None:intergenic
GATCACTATGGACAAAATCG+TGG 0.728183 7:+49536703 MsG0780038597.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG + Chr7:49536880-49536899 MsG0780038597.01.T01:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG + Chr7:49537011-49537030 MsG0780038597.01.T01:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG + Chr7:49536129-49536148 MsG0780038597.01.T01:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG + Chr7:49536169-49536188 MsG0780038597.01.T01:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG + Chr7:49536170-49536189 MsG0780038597.01.T01:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG + Chr7:49537010-49537029 MsG0780038597.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG + Chr7:49537054-49537073 MsG0780038597.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG + Chr7:49537062-49537081 MsG0780038597.01.T01:CDS 25.0%
!! TATTTTCATCTCTAATGAGA+TGG - Chr7:49537143-49537162 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG - Chr7:49536870-49536889 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG - Chr7:49536170-49536189 None:intergenic 25.0%
!! TTTGGGACAATTTAAAAGAA+AGG + Chr7:49536323-49536342 MsG0780038597.01.T01:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG - Chr7:49536273-49536292 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG - Chr7:49536846-49536865 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG + Chr7:49536306-49536325 MsG0780038597.01.T01:CDS 30.0%
ATGGTTATATTTGCGTTTAC+AGG + Chr7:49536390-49536409 MsG0780038597.01.T01:CDS 30.0%
CAAAACAACTGAACAACAAA+AGG + Chr7:49536219-49536238 MsG0780038597.01.T01:CDS 30.0%
CATGGAGATACAATTAAAGA+AGG + Chr7:49536193-49536212 MsG0780038597.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG + Chr7:49537002-49537021 MsG0780038597.01.T01:CDS 30.0%
GTCTGTAATCGAATATAACT+CGG + Chr7:49536420-49536439 MsG0780038597.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG - Chr7:49536524-49536543 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG + Chr7:49536305-49536324 MsG0780038597.01.T01:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG - Chr7:49536316-49536335 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG - Chr7:49536305-49536324 None:intergenic 30.0%
! GCTTTTGATGAAAAATCACT+CGG + Chr7:49536630-49536649 MsG0780038597.01.T01:CDS 30.0%
! TAAAGATCAGCAAGATGTTT+TGG - Chr7:49536982-49537001 None:intergenic 30.0%
! TGGAAATAATTATCTGGCTT+GGG + Chr7:49536135-49536154 MsG0780038597.01.T01:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG + Chr7:49536115-49536134 MsG0780038597.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG - Chr7:49536270-49536289 None:intergenic 30.0%
!! GGTTTTAATCGTAATCACAA+TGG + Chr7:49536796-49536815 MsG0780038597.01.T01:CDS 30.0%
!!! ACCTTTTAAAACCCTTTTCA+CGG - Chr7:49536564-49536583 None:intergenic 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG - Chr7:49537005-49537024 None:intergenic 30.0%
AACCATTCATTACGAGCATT+AGG - Chr7:49536376-49536395 None:intergenic 35.0%
AACTGAACAACAAAAGGCTA+AGG + Chr7:49536225-49536244 MsG0780038597.01.T01:CDS 35.0%
ACTTCTCTATTAACACTGTG+TGG + Chr7:49536457-49536476 MsG0780038597.01.T01:CDS 35.0%
ATTAACACTGTGTGGAGAAA+AGG + Chr7:49536465-49536484 MsG0780038597.01.T01:CDS 35.0%
ATTCATTACGAGCATTAGGA+AGG - Chr7:49536372-49536391 None:intergenic 35.0%
CTGGAAATAATTATCTGGCT+TGG + Chr7:49536134-49536153 MsG0780038597.01.T01:CDS 35.0%
TTCCTAATGCTCGTAATGAA+TGG + Chr7:49536371-49536390 MsG0780038597.01.T01:CDS 35.0%
! AGATGGTCAATCTTTTCATC+AGG - Chr7:49537126-49537145 None:intergenic 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG - Chr7:49536102-49536121 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG + Chr7:49536099-49536118 MsG0780038597.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG - Chr7:49536300-49536319 None:intergenic 35.0%
!!! ACCGTGAAAAGGGTTTTAAA+AGG + Chr7:49536560-49536579 MsG0780038597.01.T01:CDS 35.0%
AATTGTATCTCCATGCCCTT+CGG - Chr7:49536188-49536207 None:intergenic 40.0%
AGAAATCAGCAGCATCTAAG+TGG - Chr7:49537095-49537114 None:intergenic 40.0%
CAGCAACAATACCGTGAAAA+GGG + Chr7:49536550-49536569 MsG0780038597.01.T01:CDS 40.0%
CTTAATATCATGCCTGCTTG+TGG + Chr7:49536591-49536610 MsG0780038597.01.T01:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG - Chr7:49537094-49537113 None:intergenic 40.0%
GATCACTATGGACAAAATCG+TGG + Chr7:49536703-49536722 MsG0780038597.01.T01:CDS 40.0%
GTAGCAACACATGATCACTA+TGG + Chr7:49536691-49536710 MsG0780038597.01.T01:CDS 40.0%
GTGGTGACAAAGGAATATCA+TGG - Chr7:49536251-49536270 None:intergenic 40.0%
! GAAGGATGACAGTTTTCTGA+TGG - Chr7:49536354-49536373 None:intergenic 40.0%
! GTGTTGCTACATTCACTTCT+GGG - Chr7:49536681-49536700 None:intergenic 40.0%
! TATTTTGTTCTGCCACAAGC+AGG - Chr7:49536606-49536625 None:intergenic 40.0%
! TGTGTTGCTACATTCACTTC+TGG - Chr7:49536682-49536701 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG + Chr7:49536925-49536944 MsG0780038597.01.T01:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG + Chr7:49536922-49536941 MsG0780038597.01.T01:CDS 40.0%
CATTTAAATGCCGAAGGGCA+TGG + Chr7:49536175-49536194 MsG0780038597.01.T01:CDS 45.0%
CCTTATGAAGGTGGTGACAA+AGG - Chr7:49536261-49536280 None:intergenic 45.0%
CCTTTGTCACCACCTTCATA+AGG + Chr7:49536258-49536277 MsG0780038597.01.T01:CDS 45.0%
GCAGCAACAATACCGTGAAA+AGG + Chr7:49536549-49536568 MsG0780038597.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG + Chr7:49536938-49536957 MsG0780038597.01.T01:CDS 45.0%
TATGGACAAAATCGTGGTCG+TGG + Chr7:49536709-49536728 MsG0780038597.01.T01:CDS 45.0%
TGCTATCGTTGTGGAGGAAA+AGG + Chr7:49536931-49536950 MsG0780038597.01.T01:CDS 45.0%
TTTCACGGTATTGTTGCTGC+AGG - Chr7:49536549-49536568 None:intergenic 45.0%
! GCTACATTCACTTCTGGGAA+TGG - Chr7:49536676-49536695 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG - Chr7:49536971-49536990 None:intergenic 45.0%
ACGTGGTCGTGGTTATGGAA+GGG + Chr7:49536762-49536781 MsG0780038597.01.T01:CDS 50.0%
! TATGGAAGGGGACGTGGTTT+TGG + Chr7:49536775-49536794 MsG0780038597.01.T01:CDS 50.0%
CGTGGTCGTGGTTATGGAAG+GGG + Chr7:49536763-49536782 MsG0780038597.01.T01:CDS 55.0%
GACGTGGTCGTGGTTATGGA+AGG + Chr7:49536761-49536780 MsG0780038597.01.T01:CDS 55.0%
! GGGAATGGAGCACTACCAGT+GGG - Chr7:49536661-49536680 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG - Chr7:49536662-49536681 None:intergenic 55.0%
CAAAATCGTGGTCGTGGCCG+TGG + Chr7:49536715-49536734 MsG0780038597.01.T01:CDS 60.0%
CACGTCCACGACCATGACCA+CGG - Chr7:49536735-49536754 None:intergenic 60.0%
CGTGGACGTGGTCGTGGTTA+TGG + Chr7:49536757-49536776 MsG0780038597.01.T01:CDS 60.0%
CGTGGTTATGGAAGGGGACG+TGG + Chr7:49536769-49536788 MsG0780038597.01.T01:CDS 60.0%
! AATCACTCGGCTCGTCCCAC+TGG + Chr7:49536643-49536662 MsG0780038597.01.T01:CDS 60.0%
CATGGTCGTGGACGTGGACG+TGG + Chr7:49536739-49536758 MsG0780038597.01.T01:CDS 65.0%
CGTGGTCATGGTCGTGGACG+TGG + Chr7:49536733-49536752 MsG0780038597.01.T01:CDS 65.0%
CGTGGACGTGGACGTGGACG+TGG + Chr7:49536745-49536764 MsG0780038597.01.T01:CDS 70.0%
CGTGGACGTGGACGTGGTCG+TGG + Chr7:49536751-49536770 MsG0780038597.01.T01:CDS 70.0%
CGTGGCCGTGGTCATGGTCG+TGG + Chr7:49536727-49536746 MsG0780038597.01.T01:CDS 70.0%
CGTGGTCGTGGCCGTGGTCA+TGG + Chr7:49536721-49536740 MsG0780038597.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 49536091 49537173 49536091 ID=MsG0780038597.01;Name=MsG0780038597.01
Chr7 mRNA 49536091 49537173 49536091 ID=MsG0780038597.01.T01;Parent=MsG0780038597.01;Name=MsG0780038597.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|360
Chr7 exon 49536091 49537173 49536091 ID=MsG0780038597.01.T01:exon:6665;Parent=MsG0780038597.01.T01
Chr7 CDS 49536091 49537173 49536091 ID=MsG0780038597.01.T01:cds;Parent=MsG0780038597.01.T01
Gene Sequence

>MsG0780038597.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCTCTAGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACAATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTTGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTATATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGGTGACTGATGCGCAGATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCCTGCAGCAACAATACCGTGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGCCTGCTTGTGGCAGAACAAAATAAAGAGCTTTTGATGAAAAATCACTCGGCTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACACATGATCACTATGGACAAAATCGTGGTCGTGGCCGTGGTCATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTATGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGCTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGACCATCTCATTAGAGATGAAAATATCCAGAAATAG

Protein sequence

>MsG0780038597.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLCHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNKELLMKNHSARPTGSAPFPEVNVATHDHYGQNRGRGRGHGRGRGRGRGRGYGRGRGFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLADLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQK*