AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027617.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027617.01.T01 MTR_7g062720 61.268 142 55 0 1 142 1 142 7.14e-60 191
MsG0580027617.01.T01 MTR_5g061150 62.992 127 47 0 20 146 10 136 4.56e-48 159
MsG0580027617.01.T01 MTR_4g037355 57.971 138 35 5 151 287 1 116 2.57e-41 142
MsG0580027617.01.T01 MTR_4g112730 71.831 71 18 1 138 208 1 69 1.07e-26 102
MsG0580027617.01.T01 MTR_1g036690 58.537 82 27 3 280 360 5 80 1.15e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 5:-64446095 MsG0580027617.01.T01:CDS
TTCGGCATTTAAATGAATTT+TGG 0.166319 5:+64446043 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 5:-64445200 MsG0580027617.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 5:-64445208 MsG0580027617.01.T01:CDS
ATGGTTATATTTGCGTTTAC+AGG 0.259438 5:-64445820 MsG0580027617.01.T01:CDS
TATGGAAGGGGACGTGGTTT+TGG 0.259444 5:-64445435 MsG0580027617.01.T01:CDS
AAATTGTCCCAAAGATCTTT+TGG 0.267031 5:+64445897 None:intergenic
TATTTCTGGAAATAATTATC+TGG 0.282933 5:-64446081 MsG0580027617.01.T01:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.283634 5:-64445905 MsG0580027617.01.T01:CDS
TGTGTTGCTACATTCACTTC+TGG 0.294995 5:+64445531 None:intergenic
TATAGGTGGGGTTCTATTCC+TGG 0.331534 5:+64445046 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 5:+64445231 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 5:-64445148 MsG0580027617.01.T01:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 5:-64446076 MsG0580027617.01.T01:CDS
TGGAAATAATTATCTGGCTT+GGG 0.353242 5:-64446075 MsG0580027617.01.T01:CDS
CGTGGACGTGGTCGTGGTTA+TGG 0.362984 5:-64445453 MsG0580027617.01.T01:CDS
AACCATTCATTACGAGCATT+AGG 0.386252 5:+64445837 None:intergenic
GAAAATGATGAAGATGATTA+TGG 0.391347 5:-64445156 MsG0580027617.01.T01:CDS
TTTGGGACAATTTAAAAGAA+AGG 0.395342 5:-64445887 MsG0580027617.01.T01:CDS
AGAAGATCCAAAAGATCTTT+GGG 0.400056 5:-64445904 MsG0580027617.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 5:-64445279 MsG0580027617.01.T01:CDS
GTGTTGCTACATTCACTTCT+GGG 0.423307 5:+64445532 None:intergenic
CGTGGTTATGGAAGGGGACG+TGG 0.429618 5:-64445441 MsG0580027617.01.T01:CDS
CCTTCGTCACCACCTTCATA+AGG 0.433246 5:-64445952 MsG0580027617.01.T01:CDS
ATTAACACTGTGTGGAGAAA+AGG 0.440407 5:-64445745 MsG0580027617.01.T01:CDS
CAAAATCGTGGTCGTGGCCG+TGG 0.442209 5:-64445495 MsG0580027617.01.T01:CDS
CGTGGTCGTGGCCGTGGTCA+TGG 0.454221 5:-64445489 MsG0580027617.01.T01:CDS
CAAAACAACTGAACAACAAA+AGG 0.454927 5:-64445991 MsG0580027617.01.T01:CDS
AATTGTATCTCCATGCCCTT+CGG 0.460742 5:+64446025 None:intergenic
AGAAATCAGCAGCATCTAAG+TGG 0.464577 5:+64445118 None:intergenic
TTTCACGGTATTGTTGCTGC+AGG 0.465441 5:+64445664 None:intergenic
AATGTTGTAAAGAATGATAT+TGG 0.471241 5:-64445330 MsG0580027617.01.T01:CDS
GCAGCAACAATACCGTGAAA+AGG 0.474876 5:-64445661 MsG0580027617.01.T01:CDS
AATCACTCGGCTCGTCCCAC+TGG 0.475930 5:-64445567 MsG0580027617.01.T01:CDS
GCTACATTCACTTCTGGGAA+TGG 0.483138 5:+64445537 None:intergenic
ACTTCAAAATAAGGATAAAA+GGG 0.487291 5:-64445199 MsG0580027617.01.T01:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 5:-64446035 MsG0580027617.01.T01:CDS
CAGCAACAATACCGTGAAAA+GGG 0.498612 5:-64445660 MsG0580027617.01.T01:CDS
GACGTGGTCGTGGTTATGGA+AGG 0.501950 5:-64445449 MsG0580027617.01.T01:CDS
GTAGCAACACATGATCACTA+TGG 0.504040 5:-64445519 MsG0580027617.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 5:-64445272 MsG0580027617.01.T01:CDS
CGTGGCCGTGGTCATGGTCG+TGG 0.506333 5:-64445483 MsG0580027617.01.T01:CDS
TTCCTAATGCTCGTAATGAA+TGG 0.506622 5:-64445839 MsG0580027617.01.T01:CDS
TACATTAGATGCATGAAAAG+TGG 0.516304 5:+64445689 None:intergenic
CATGGTCGTGGACGTGGACG+TGG 0.524744 5:-64445471 MsG0580027617.01.T01:CDS
CGTGGACGTGGACGTGGTCG+TGG 0.524981 5:-64445459 MsG0580027617.01.T01:CDS
CATTAGAGATGAAAATATCC+AGG 0.532865 5:-64445064 MsG0580027617.01.T01:CDS
CATGGAGATACAATTAAAGA+AGG 0.533844 5:-64446017 MsG0580027617.01.T01:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 5:-64446041 MsG0580027617.01.T01:CDS
CGTGGTCATGGTCGTGGACG+TGG 0.540833 5:-64445477 MsG0580027617.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.546682 5:-64445465 MsG0580027617.01.T01:CDS
CGTGGTCGTGGTTATGGAAG+GGG 0.551769 5:-64445447 MsG0580027617.01.T01:CDS
CTTAATATCATGCCTGCTTG+TGG 0.567363 5:-64445619 MsG0580027617.01.T01:CDS
TATGGACAAAATCGTGGTCG+TGG 0.573891 5:-64445501 MsG0580027617.01.T01:CDS
GTCTGTAATCGAATATAACT+CGG 0.574961 5:-64445790 MsG0580027617.01.T01:CDS
AACTGAACAACAAAAGGCTA+AGG 0.582581 5:-64445985 MsG0580027617.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 5:-64445288 MsG0580027617.01.T01:CDS
ATTCATTACGAGCATTAGGA+AGG 0.589984 5:+64445841 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 5:+64445952 None:intergenic
ACGTGGTCGTGGTTATGGAA+GGG 0.598966 5:-64445448 MsG0580027617.01.T01:CDS
AGTATTTGCTATCGTTGTGG+AGG 0.599695 5:-64445285 MsG0580027617.01.T01:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 5:+64445119 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 5:-64446040 MsG0580027617.01.T01:CDS
CACGTCCACGACCATGACCA+CGG 0.661673 5:+64445478 None:intergenic
GTGGTGACGAAGGAATATCA+TGG 0.663314 5:+64445962 None:intergenic
ACTTCTCTATTAACACTGTG+TGG 0.668278 5:-64445753 MsG0580027617.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 5:+64445551 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 5:+64445552 None:intergenic
GATCACTATGGACAAAATCG+TGG 0.728183 5:-64445507 MsG0580027617.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG - Chr5:64445848-64445867 MsG0580027617.01.T01:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG - Chr5:64445979-64445998 MsG0580027617.01.T01:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG - Chr5:64445097-64445116 MsG0580027617.01.T01:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG - Chr5:64445137-64445156 MsG0580027617.01.T01:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG - Chr5:64445138-64445157 MsG0580027617.01.T01:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG - Chr5:64445978-64445997 MsG0580027617.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG - Chr5:64446022-64446041 MsG0580027617.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG - Chr5:64446030-64446049 MsG0580027617.01.T01:CDS 25.0%
!! TAAAGATCAACAAGATGTTT+TGG + Chr5:64445950-64445969 None:intergenic 25.0%
!! TATTTTCATCTCTAATGAGA+TGG + Chr5:64446111-64446130 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG + Chr5:64445838-64445857 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG + Chr5:64445138-64445157 None:intergenic 25.0%
!! TTTGGGACAATTTAAAAGAA+AGG - Chr5:64445291-64445310 MsG0580027617.01.T01:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG + Chr5:64445241-64445260 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG + Chr5:64445814-64445833 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG - Chr5:64445274-64445293 MsG0580027617.01.T01:CDS 30.0%
ATGGTTATATTTGCGTTTAC+AGG - Chr5:64445358-64445377 MsG0580027617.01.T01:CDS 30.0%
CAAAACAACTGAACAACAAA+AGG - Chr5:64445187-64445206 MsG0580027617.01.T01:CDS 30.0%
CATGGAGATACAATTAAAGA+AGG - Chr5:64445161-64445180 MsG0580027617.01.T01:CDS 30.0%
CATTAGAGATGAAAATATCC+AGG - Chr5:64446114-64446133 MsG0580027617.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG - Chr5:64445970-64445989 MsG0580027617.01.T01:CDS 30.0%
GTCTGTAATCGAATATAACT+CGG - Chr5:64445388-64445407 MsG0580027617.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG + Chr5:64445492-64445511 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG - Chr5:64445273-64445292 MsG0580027617.01.T01:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG + Chr5:64445284-64445303 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG + Chr5:64445273-64445292 None:intergenic 30.0%
! GCTTTTGATGAAAAATCACT+CGG - Chr5:64445598-64445617 MsG0580027617.01.T01:CDS 30.0%
! TGGAAATAATTATCTGGCTT+GGG - Chr5:64445103-64445122 MsG0580027617.01.T01:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG - Chr5:64445083-64445102 MsG0580027617.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG + Chr5:64445238-64445257 None:intergenic 30.0%
!! GGTTTTAATCGTAATCACAA+TGG - Chr5:64445764-64445783 MsG0580027617.01.T01:CDS 30.0%
!!! ACCTTTTAAAACCCTTTTCA+CGG + Chr5:64445532-64445551 None:intergenic 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG + Chr5:64445973-64445992 None:intergenic 30.0%
AACCATTCATTACGAGCATT+AGG + Chr5:64445344-64445363 None:intergenic 35.0%
AACTGAACAACAAAAGGCTA+AGG - Chr5:64445193-64445212 MsG0580027617.01.T01:CDS 35.0%
ACTTCTCTATTAACACTGTG+TGG - Chr5:64445425-64445444 MsG0580027617.01.T01:CDS 35.0%
ATTAACACTGTGTGGAGAAA+AGG - Chr5:64445433-64445452 MsG0580027617.01.T01:CDS 35.0%
ATTCATTACGAGCATTAGGA+AGG + Chr5:64445340-64445359 None:intergenic 35.0%
CTGGAAATAATTATCTGGCT+TGG - Chr5:64445102-64445121 MsG0580027617.01.T01:CDS 35.0%
TTCCTAATGCTCGTAATGAA+TGG - Chr5:64445339-64445358 MsG0580027617.01.T01:CDS 35.0%
! AGATGGTCAATCTTTTCATC+AGG + Chr5:64446094-64446113 None:intergenic 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG + Chr5:64445070-64445089 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG - Chr5:64445067-64445086 MsG0580027617.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG + Chr5:64445268-64445287 None:intergenic 35.0%
!!! ACCGTGAAAAGGGTTTTAAA+AGG - Chr5:64445528-64445547 MsG0580027617.01.T01:CDS 35.0%
AATTGTATCTCCATGCCCTT+CGG + Chr5:64445156-64445175 None:intergenic 40.0%
AGAAATCAGCAGCATCTAAG+TGG + Chr5:64446063-64446082 None:intergenic 40.0%
CAGCAACAATACCGTGAAAA+GGG - Chr5:64445518-64445537 MsG0580027617.01.T01:CDS 40.0%
CTTAATATCATGCCTGCTTG+TGG - Chr5:64445559-64445578 MsG0580027617.01.T01:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG + Chr5:64446062-64446081 None:intergenic 40.0%
GATCACTATGGACAAAATCG+TGG - Chr5:64445671-64445690 MsG0580027617.01.T01:CDS 40.0%
GTAGCAACACATGATCACTA+TGG - Chr5:64445659-64445678 MsG0580027617.01.T01:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG + Chr5:64445322-64445341 None:intergenic 40.0%
! GTGTTGCTACATTCACTTCT+GGG + Chr5:64445649-64445668 None:intergenic 40.0%
! TATTTTGTTCTGCCACAAGC+AGG + Chr5:64445574-64445593 None:intergenic 40.0%
! TGTGTTGCTACATTCACTTC+TGG + Chr5:64445650-64445669 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG - Chr5:64445893-64445912 MsG0580027617.01.T01:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG - Chr5:64445890-64445909 MsG0580027617.01.T01:CDS 40.0%
CATTTAAATGCCGAAGGGCA+TGG - Chr5:64445143-64445162 MsG0580027617.01.T01:CDS 45.0%
GCAGCAACAATACCGTGAAA+AGG - Chr5:64445517-64445536 MsG0580027617.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG - Chr5:64445906-64445925 MsG0580027617.01.T01:CDS 45.0%
TATGGACAAAATCGTGGTCG+TGG - Chr5:64445677-64445696 MsG0580027617.01.T01:CDS 45.0%
TGCTATCGTTGTGGAGGAAA+AGG - Chr5:64445899-64445918 MsG0580027617.01.T01:CDS 45.0%
TTTCACGGTATTGTTGCTGC+AGG + Chr5:64445517-64445536 None:intergenic 45.0%
! GCTACATTCACTTCTGGGAA+TGG + Chr5:64445644-64445663 None:intergenic 45.0%
! GTGGTGACGAAGGAATATCA+TGG + Chr5:64445219-64445238 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG + Chr5:64445939-64445958 None:intergenic 45.0%
ACGTGGTCGTGGTTATGGAA+GGG - Chr5:64445730-64445749 MsG0580027617.01.T01:CDS 50.0%
CCTTCGTCACCACCTTCATA+AGG - Chr5:64445226-64445245 MsG0580027617.01.T01:CDS 50.0%
! CCTTATGAAGGTGGTGACGA+AGG + Chr5:64445229-64445248 None:intergenic 50.0%
! TATGGAAGGGGACGTGGTTT+TGG - Chr5:64445743-64445762 MsG0580027617.01.T01:CDS 50.0%
CGTGGTCGTGGTTATGGAAG+GGG - Chr5:64445731-64445750 MsG0580027617.01.T01:CDS 55.0%
GACGTGGTCGTGGTTATGGA+AGG - Chr5:64445729-64445748 MsG0580027617.01.T01:CDS 55.0%
! GGGAATGGAGCACTACCAGT+GGG + Chr5:64445629-64445648 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG + Chr5:64445630-64445649 None:intergenic 55.0%
CAAAATCGTGGTCGTGGCCG+TGG - Chr5:64445683-64445702 MsG0580027617.01.T01:CDS 60.0%
CACGTCCACGACCATGACCA+CGG + Chr5:64445703-64445722 None:intergenic 60.0%
CGTGGACGTGGTCGTGGTTA+TGG - Chr5:64445725-64445744 MsG0580027617.01.T01:CDS 60.0%
CGTGGTTATGGAAGGGGACG+TGG - Chr5:64445737-64445756 MsG0580027617.01.T01:CDS 60.0%
! AATCACTCGGCTCGTCCCAC+TGG - Chr5:64445611-64445630 MsG0580027617.01.T01:CDS 60.0%
CATGGTCGTGGACGTGGACG+TGG - Chr5:64445707-64445726 MsG0580027617.01.T01:CDS 65.0%
CGTGGTCATGGTCGTGGACG+TGG - Chr5:64445701-64445720 MsG0580027617.01.T01:CDS 65.0%
CGTGGACGTGGACGTGGACG+TGG - Chr5:64445713-64445732 MsG0580027617.01.T01:CDS 70.0%
CGTGGACGTGGACGTGGTCG+TGG - Chr5:64445719-64445738 MsG0580027617.01.T01:CDS 70.0%
CGTGGCCGTGGTCATGGTCG+TGG - Chr5:64445695-64445714 MsG0580027617.01.T01:CDS 70.0%
CGTGGTCGTGGCCGTGGTCA+TGG - Chr5:64445689-64445708 MsG0580027617.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 64445059 64446141 64445059 ID=MsG0580027617.01;Name=MsG0580027617.01
Chr5 mRNA 64445059 64446141 64445059 ID=MsG0580027617.01.T01;Parent=MsG0580027617.01;Name=MsG0580027617.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|360
Chr5 exon 64445059 64446141 64445059 ID=MsG0580027617.01.T01:exon:23091;Parent=MsG0580027617.01.T01
Chr5 CDS 64445059 64446141 64445059 ID=MsG0580027617.01.T01:cds;Parent=MsG0580027617.01.T01
Gene Sequence

>MsG0580027617.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCTCTAGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACAATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTATATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGGTGACTGATGTGCAGATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCCTGCAGCAACAATACCGTGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGCCTGCTTGTGGCAGAACAAAATAACGAGCTTTTGATGAAAAATCACTCGGCTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACACATGATCACTATGGACAAAATCGTGGTCGTGGCCGTGGTCATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTATGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGACCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MsG0580027617.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDVQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHSARPTGSAPFPEVNVATHDHYGQNRGRGRGHGRGRGRGRGRGYGRGRGFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE*