Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041631.01.T01 | BAE71305.1 | 97.26 | 73 | 2 | 0 | 1 | 73 | 230 | 302 | 5.41E-46 | 158 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041631.01.T01 | Q05212 | 79.412 | 68 | 14 | 0 | 1 | 68 | 242 | 309 | 5.16E-36 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041631.01.T01 | Q2PEP7 | 97.260 | 73 | 2 | 0 | 1 | 73 | 230 | 302 | 2.59e-46 | 158 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049068.01 | MsG0780041631.01 | 0.805270 | 1.447297e-49 | 7.623928e-47 |
| MsG0180000246.01 | MsG0780041631.01 | 0.806536 | 7.842030e-50 | 4.267184e-47 |
| MsG0180000785.01 | MsG0780041631.01 | 0.822014 | 2.970309e-53 | 2.456125e-50 |
| MsG0780041091.01 | MsG0780041631.01 | 0.817920 | 2.566913e-52 | 1.894282e-49 |
| MsG0780041631.01 | MsG0880044392.01 | 0.803145 | 4.008157e-49 | 1.999043e-46 |
| MsG0780041631.01 | MsG0880045044.01 | 0.819736 | 9.928168e-53 | 7.703909e-50 |
| MsG0780041631.01 | MsG0880047322.01 | 0.804278 | 2.332642e-49 | 1.197758e-46 |
| MsG0280010564.01 | MsG0780041631.01 | 0.805250 | 1.461477e-49 | 7.694551e-47 |
| MsG0680031716.01 | MsG0780041631.01 | -0.803860 | 2.849831e-49 | 1.447747e-46 |
| MsG0680034766.01 | MsG0780041631.01 | 0.806351 | 8.580931e-50 | 4.647072e-47 |
| MsG0680034767.01 | MsG0780041631.01 | 0.818857 | 1.574482e-52 | 1.192353e-49 |
| MsG0480018848.01 | MsG0780041631.01 | 0.805454 | 1.324636e-49 | 7.010990e-47 |
| MsG0580024873.01 | MsG0780041631.01 | 0.809770 | 1.605775e-50 | 9.511937e-48 |
| MsG0580025607.01 | MsG0780041631.01 | 0.808607 | 2.849622e-50 | 1.636484e-47 |
| MsG0380016864.01 | MsG0780041631.01 | 0.831366 | 1.738497e-55 | 1.882562e-52 |
| MsG0580028024.01 | MsG0780041631.01 | 0.809729 | 1.638122e-50 | 9.693103e-48 |
| MsG0580029734.01 | MsG0780041631.01 | 0.803370 | 3.601027e-49 | 1.806372e-46 |
| MsG0780038056.01 | MsG0780041631.01 | 0.821793 | 3.342773e-53 | 2.746908e-50 |
| MsG0780038639.01 | MsG0780041631.01 | 0.800921 | 1.148418e-48 | 5.413576e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041631.01.T01 | MTR_7g114750 | 95.890 | 73 | 3 | 0 | 1 | 73 | 232 | 304 | 4.94e-47 | 151 |
| MsG0780041631.01.T01 | MTR_7g114750 | 96.721 | 61 | 2 | 0 | 1 | 61 | 232 | 292 | 8.61e-42 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041631.01.T01 | AT3G04880 | 79.412 | 68 | 14 | 0 | 1 | 68 | 242 | 309 | 5.26e-37 | 125 |
Find 15 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGCAATTGATAAGGAATT+AGG | 0.203217 | 7:-93260510 | MsG0780041631.01.T01:CDS |
| TCATCAAATGGGATGGTCAT+TGG | 0.341138 | 7:-93260569 | MsG0780041631.01.T01:CDS |
| TTTGATGAGGATCTTGAAAC+TGG | 0.372349 | 7:-93260537 | MsG0780041631.01.T01:CDS |
| CATCAAATGGGATGGTCATT+GGG | 0.378212 | 7:-93260568 | MsG0780041631.01.T01:CDS |
| TGGAAGGGAAGAAGAGTGTT+TGG | 0.378608 | 7:-93260695 | MsG0780041631.01.T01:CDS |
| AGAGATATGATCTAACGGTT+GGG | 0.464266 | 7:-93260656 | MsG0780041631.01.T01:CDS |
| GATTACTTGTTTACACCTGC+TGG | 0.491415 | 7:-93260633 | MsG0780041631.01.T01:CDS |
| GAGAGATATGATCTAACGGT+TGG | 0.501797 | 7:-93260657 | MsG0780041631.01.T01:CDS |
| TGTTTGGAATTTGACAAAAG+AGG | 0.505958 | 7:-93260679 | MsG0780041631.01.T01:CDS |
| CATGATTTGGTGGTGATGGA+AGG | 0.508786 | 7:-93260711 | None:intergenic |
| ATGATTTGGTGGTGATGGAA+GGG | 0.521433 | 7:-93260710 | None:intergenic |
| GAGATATGATCTAACGGTTG+GGG | 0.546777 | 7:-93260655 | MsG0780041631.01.T01:CDS |
| ACTCTATGCACATCACCAGC+AGG | 0.587588 | 7:+93260618 | None:intergenic |
| AGAGGAGAGATATGATCTAA+CGG | 0.623308 | 7:-93260661 | MsG0780041631.01.T01:CDS |
| GCATAGAGTGAAATATCATG+AGG | 0.703910 | 7:-93260604 | MsG0780041631.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGCAATTGATAAGGAATT+AGG | - | Chr7:93260683-93260702 | MsG0780041631.01.T01:CDS | 25.0% |
| ! | TGGAAAAATTGCAATTGATA+AGG | - | Chr7:93260676-93260695 | MsG0780041631.01.T01:CDS | 25.0% |
| !! | ATACTGAGTTTTTCATCAAA+TGG | - | Chr7:93260612-93260631 | MsG0780041631.01.T01:CDS | 25.0% |
| !! | TACTGAGTTTTTCATCAAAT+GGG | - | Chr7:93260613-93260632 | MsG0780041631.01.T01:CDS | 25.0% |
| ! | TGTTTGGAATTTGACAAAAG+AGG | - | Chr7:93260514-93260533 | MsG0780041631.01.T01:CDS | 30.0% |
| !!! | TTGGGATATGTTTTTTGATG+AGG | - | Chr7:93260643-93260662 | MsG0780041631.01.T01:CDS | 30.0% |
| AGAGATATGATCTAACGGTT+GGG | - | Chr7:93260537-93260556 | MsG0780041631.01.T01:CDS | 35.0% | |
| AGAGGAGAGATATGATCTAA+CGG | - | Chr7:93260532-93260551 | MsG0780041631.01.T01:CDS | 35.0% | |
| GCATAGAGTGAAATATCATG+AGG | - | Chr7:93260589-93260608 | MsG0780041631.01.T01:CDS | 35.0% | |
| TTTGATGAGGATCTTGAAAC+TGG | - | Chr7:93260656-93260675 | MsG0780041631.01.T01:CDS | 35.0% | |
| ! | GAGTTTTTCATCAAATGGGA+TGG | - | Chr7:93260617-93260636 | MsG0780041631.01.T01:CDS | 35.0% |
| CATCAAATGGGATGGTCATT+GGG | - | Chr7:93260625-93260644 | MsG0780041631.01.T01:CDS | 40.0% | |
| GAGAGATATGATCTAACGGT+TGG | - | Chr7:93260536-93260555 | MsG0780041631.01.T01:CDS | 40.0% | |
| GAGATATGATCTAACGGTTG+GGG | - | Chr7:93260538-93260557 | MsG0780041631.01.T01:CDS | 40.0% | |
| GATTACTTGTTTACACCTGC+TGG | - | Chr7:93260560-93260579 | MsG0780041631.01.T01:CDS | 40.0% | |
| TCATCAAATGGGATGGTCAT+TGG | - | Chr7:93260624-93260643 | MsG0780041631.01.T01:CDS | 40.0% | |
| !! | TGGAAGGGAAGAAGAGTGTT+TGG | - | Chr7:93260498-93260517 | MsG0780041631.01.T01:CDS | 45.0% |
| ACTCTATGCACATCACCAGC+AGG | + | Chr7:93260578-93260597 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 93260497 | 93260718 | 93260497 | ID=MsG0780041631.01;Name=MsG0780041631.01 |
| Chr7 | mRNA | 93260497 | 93260718 | 93260497 | ID=MsG0780041631.01.T01;Parent=MsG0780041631.01;Name=MsG0780041631.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|73 |
| Chr7 | exon | 93260497 | 93260718 | 93260497 | ID=MsG0780041631.01.T01:exon:5226;Parent=MsG0780041631.01.T01 |
| Chr7 | CDS | 93260497 | 93260718 | 93260497 | ID=MsG0780041631.01.T01:cds;Parent=MsG0780041631.01.T01 |
| Gene Sequence |
| Protein sequence |
