AlfalfaGEDB | MsG0680031716.01

M. sativa cultivar ZhongmuNo.1 / MsG0680031716.01

KEGG: -
GO: -
PFAM: PF02338

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031716.01.T01	XP_013451591.1	93.304	224	7	1	1	224	1	216	1.81E-154	441

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031716.01.T01	Q9SGA5	75.622	201	41	1	24	224	13	205	1.33E-115	335

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031716.01.T01	A0A072U777	93.304	224	7	1	1	224	1	216	8.66e-155	441

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047899.01	MsG0680031716.01	-0.806961	6.377874e-50	3.508597e-47
MsG0080048727.01	MsG0680031716.01	-0.803937	2.746956e-49	1.398326e-46
MsG0080048773.01	MsG0680031716.01	-0.814987	1.164374e-51	7.929363e-49
MsG0080048878.01	MsG0680031716.01	-0.828808	7.316823e-55	7.350911e-52
MsG0080049039.01	MsG0680031716.01	-0.804398	2.202536e-49	1.134413e-46
MsG0080049040.01	MsG0680031716.01	-0.847701	9.792542e-60	1.755356e-56
MsG0080049068.01	MsG0680031716.01	-0.849771	2.610640e-60	5.003087e-57
MsG0180000113.01	MsG0680031716.01	-0.802183	6.329852e-49	3.080517e-46
MsG0180000188.01	MsG0680031716.01	0.812547	4.012275e-51	2.558569e-48
MsG0180001085.01	MsG0680031716.01	-0.818908	1.533524e-52	1.162975e-49
MsG0180001175.01	MsG0680031716.01	-0.810204	1.294985e-50	7.757752e-48
MsG0180001270.01	MsG0680031716.01	-0.832518	9.033221e-56	1.012047e-52
MsG0180001610.01	MsG0680031716.01	-0.818920	1.523450e-52	1.155768e-49
MsG0180001722.01	MsG0680031716.01	-0.814142	1.791048e-51	1.192168e-48
MsG0180002700.01	MsG0680031716.01	-0.819808	9.560151e-53	7.433637e-50
MsG0180003353.01	MsG0680031716.01	-0.815958	7.080725e-52	4.951721e-49
MsG0180003821.01	MsG0680031716.01	-0.811471	6.887591e-51	4.267185e-48
MsG0180004115.01	MsG0680031716.01	-0.800957	1.129416e-48	5.328825e-46
MsG0180004192.01	MsG0680031716.01	-0.841285	5.212447e-58	7.621831e-55
MsG0180004276.01	MsG0680031716.01	-0.803092	4.110176e-49	2.047087e-46
MsG0180004448.01	MsG0680031716.01	-0.817525	3.151386e-52	2.300476e-49
MsG0180004466.01	MsG0680031716.01	-0.842059	3.258009e-58	4.880997e-55
MsG0180004759.01	MsG0680031716.01	0.853302	2.613803e-61	5.621540e-58
MsG0180005349.01	MsG0680031716.01	-0.825054	5.778693e-54	5.210086e-51
MsG0180005395.01	MsG0680031716.01	0.846074	2.728682e-59	4.641644e-56
MsG0180005750.01	MsG0680031716.01	-0.802284	6.035670e-49	2.945098e-46
MsG0180005807.01	MsG0680031716.01	-0.809139	2.193124e-50	1.277446e-47
MsG0180005961.01	MsG0680031716.01	-0.801895	7.255821e-49	3.505579e-46
MsG0180005964.01	MsG0680031716.01	-0.842339	2.747701e-58	4.153243e-55
MsG0180006031.01	MsG0680031716.01	-0.814061	1.865933e-51	1.239299e-48
MsG0380012868.01	MsG0680031716.01	-0.807858	4.115730e-50	2.317507e-47
MsG0380014297.01	MsG0680031716.01	0.844637	6.682248e-59	1.085542e-55
MsG0680030784.01	MsG0680031716.01	-0.801941	7.098026e-49	3.433155e-46
MsG0680030908.01	MsG0680031716.01	-0.802018	6.844702e-49	3.317189e-46
MsG0680031469.01	MsG0680031716.01	-0.821522	3.860710e-53	3.148178e-50
MsG0680031716.01	MsG0680031826.01	-0.813297	2.749043e-51	1.788320e-48
MsG0680031716.01	MsG0680032473.01	0.806971	6.348844e-50	3.493473e-47
MsG0680031716.01	MsG0680033377.01	-0.848099	7.603538e-60	1.379952e-56
MsG0680031716.01	MsG0680034064.01	-0.827893	1.216283e-54	1.189871e-51
MsG0680031716.01	MsG0780035992.01	-0.830762	2.445965e-55	2.601752e-52
MsG0680031716.01	MsG0780036711.01	-0.801660	8.107438e-49	3.893507e-46
MsG0680031716.01	MsG0780036715.01	-0.805671	1.192645e-49	6.347802e-47
MsG0680031716.01	MsG0780038056.01	-0.816630	5.007393e-52	3.566847e-49
MsG0680031716.01	MsG0780038351.01	-0.823421	1.397903e-53	1.202975e-50
MsG0680031716.01	MsG0780038584.01	0.805197	1.499254e-49	7.882937e-47
MsG0680031716.01	MsG0780038616.01	-0.821886	3.180288e-53	2.620362e-50
MsG0680031716.01	MsG0780038784.01	-0.827932	1.190138e-54	1.165634e-51
MsG0680031716.01	MsG0780038846.01	-0.806434	8.242869e-50	4.473453e-47
MsG0680031716.01	MsG0780039675.01	-0.819242	1.286911e-52	9.849508e-50
MsG0680031716.01	MsG0780039746.01	-0.807628	4.606356e-50	2.578344e-47
MsG0680031716.01	MsG0780039853.01	-0.806544	7.814428e-50	4.253009e-47
MsG0680031716.01	MsG0780040277.01	-0.836528	8.878062e-57	1.121783e-53
MsG0680031716.01	MsG0780040284.01	-0.824278	8.801746e-54	7.760044e-51
MsG0680031716.01	MsG0780040388.01	-0.829810	4.178879e-55	4.324476e-52
MsG0680031716.01	MsG0780041138.01	-0.805204	1.494709e-49	7.860356e-47
MsG0680031716.01	MsG0780041631.01	-0.803860	2.849831e-49	1.447747e-46
MsG0680031716.01	MsG0880041947.01	-0.837326	5.552681e-57	7.187769e-54
MsG0680031716.01	MsG0880042890.01	-0.831808	1.353232e-55	1.484877e-52
MsG0680031716.01	MsG0880043024.01	-0.855275	7.039410e-62	1.616380e-58
MsG0680031716.01	MsG0880043174.01	-0.801562	8.490251e-49	4.067303e-46
MsG0680031716.01	MsG0880043277.01	-0.833195	6.130978e-56	7.009566e-53
MsG0680031716.01	MsG0880044142.01	-0.802442	5.599890e-49	2.743406e-46
MsG0680031716.01	MsG0880044392.01	-0.853432	2.399720e-61	5.184416e-58
MsG0680031716.01	MsG0880044487.01	-0.811868	5.645821e-51	3.534682e-48
MsG0680031716.01	MsG0880045101.01	-0.829907	3.958653e-55	4.107700e-52
MsG0680031716.01	MsG0880046054.01	-0.847938	8.423609e-60	1.521264e-56
MsG0680031716.01	MsG0880046334.01	0.803693	3.085600e-49	1.560792e-46
MsG0680031716.01	MsG0880046382.01	-0.868131	8.199545e-66	2.933660e-62
MsG0680031716.01	MsG0880046765.01	-0.831946	1.251310e-55	1.378549e-52
MsG0680031716.01	MsG0880047422.01	0.855922	4.557104e-62	1.069213e-58
MsG0680031716.01	MsG0880047587.01	-0.820812	5.627962e-53	4.499628e-50
MsG0680031716.01	MsG0880047656.01	-0.833032	6.733082e-56	7.660605e-53
MsG0680031716.01	MsG0880047703.01	-0.814795	1.284221e-51	8.700396e-49
MsG0480018584.01	MsG0680031716.01	-0.803696	3.081888e-49	1.559019e-46
MsG0480018644.01	MsG0680031716.01	-0.834682	2.603072e-56	3.111356e-53
MsG0480018850.01	MsG0680031716.01	-0.824209	9.137886e-54	8.041116e-51
MsG0480019340.01	MsG0680031716.01	-0.841737	3.962419e-58	5.876250e-55
MsG0480020315.01	MsG0680031716.01	-0.803162	3.976279e-49	1.984080e-46
MsG0480020408.01	MsG0680031716.01	-0.811096	8.304188e-51	5.094262e-48
MsG0480020690.01	MsG0680031716.01	-0.851188	1.043991e-60	2.096188e-57
MsG0480020758.01	MsG0680031716.01	-0.829776	4.260877e-55	4.404644e-52
MsG0480020889.01	MsG0680031716.01	-0.812794	3.544332e-51	2.274575e-48
MsG0480021248.01	MsG0680031716.01	-0.801507	8.715258e-49	4.169368e-46
MsG0480021803.01	MsG0680031716.01	-0.806991	6.286831e-50	3.461106e-47
MsG0480021822.01	MsG0680031716.01	0.800958	1.128888e-48	5.326444e-46
MsG0480022149.01	MsG0680031716.01	-0.828365	9.363362e-55	9.286829e-52
MsG0480022647.01	MsG0680031716.01	-0.835524	1.596238e-56	1.956905e-53
MsG0480023073.01	MsG0680031716.01	-0.804272	2.339362e-49	1.201016e-46
MsG0480023314.01	MsG0680031716.01	-0.851418	8.990710e-61	1.818762e-57
MsG0480023553.01	MsG0680031716.01	-0.816695	4.844404e-52	3.456738e-49
MsG0480023992.01	MsG0680031716.01	-0.801881	7.304482e-49	3.527845e-46
MsG0580024873.01	MsG0680031716.01	-0.826003	3.442979e-54	3.189223e-51
MsG0580025366.01	MsG0680031716.01	-0.839321	1.699575e-57	2.338632e-54
MsG0580025502.01	MsG0680031716.01	-0.837141	6.192957e-57	7.971278e-54
MsG0580025561.01	MsG0680031716.01	0.826029	3.394831e-54	3.146866e-51
MsG0580025607.01	MsG0680031716.01	-0.835401	1.714947e-56	2.094870e-53
MsG0580025627.01	MsG0680031716.01	-0.842195	2.999344e-58	4.512460e-55
MsG0580025668.01	MsG0680031716.01	-0.843002	1.831419e-58	2.825849e-55
MsG0580025971.01	MsG0680031716.01	-0.801524	8.647734e-49	4.138812e-46
MsG0380015603.01	MsG0680031716.01	-0.814493	1.498100e-51	1.006758e-48
MsG0380016057.01	MsG0680031716.01	-0.806486	8.036275e-50	4.367211e-47
MsG0380016192.01	MsG0680031716.01	-0.844286	8.305143e-59	1.334407e-55
MsG0380016194.01	MsG0680031716.01	-0.812465	4.181831e-51	2.660992e-48
MsG0380016284.01	MsG0680031716.01	-0.809152	2.179196e-50	1.269769e-47
MsG0380016347.01	MsG0680031716.01	0.825345	4.931716e-54	4.483230e-51
MsG0380016425.01	MsG0680031716.01	0.804551	2.045921e-49	1.057932e-46
MsG0380016540.01	MsG0680031716.01	-0.811316	7.442242e-51	4.591715e-48
MsG0380016820.01	MsG0680031716.01	-0.804843	1.777840e-49	9.263540e-47
MsG0380016864.01	MsG0680031716.01	-0.835475	1.642606e-56	2.010798e-53
MsG0380017087.01	MsG0680031716.01	-0.828731	7.639452e-55	7.657548e-52
MsG0380017088.01	MsG0680031716.01	-0.816754	4.698827e-52	3.358424e-49
MsG0380017703.01	MsG0680031716.01	-0.870006	2.020523e-66	7.738412e-63
MsG0380017785.01	MsG0680031716.01	-0.800870	1.176682e-48	5.539422e-46
MsG0380017968.01	MsG0680031716.01	-0.823594	1.273369e-53	1.101267e-50
MsG0380018037.01	MsG0680031716.01	-0.807609	4.649100e-50	2.600903e-47
MsG0380018061.01	MsG0680031716.01	-0.814440	1.538714e-51	1.032549e-48
MsG0380018069.01	MsG0680031716.01	-0.836447	9.307597e-57	1.173317e-53
MsG0480018091.01	MsG0680031716.01	0.803078	4.139376e-49	2.060865e-46
MsG0580026159.01	MsG0680031716.01	0.804701	1.903772e-49	9.882684e-47
MsG0580027394.01	MsG0680031716.01	-0.830796	2.400024e-55	2.555423e-52
MsG0580028912.01	MsG0680031716.01	0.807762	4.315164e-50	2.423745e-47
MsG0580029734.01	MsG0680031716.01	-0.822726	2.030047e-53	1.713211e-50
MsG0680030516.01	MsG0680031716.01	-0.827802	1.279265e-54	1.248042e-51
MsG0280006996.01	MsG0680031716.01	-0.827607	1.425141e-54	1.382466e-51
MsG0280007075.01	MsG0680031716.01	-0.817596	3.038189e-52	2.222138e-49
MsG0280007502.01	MsG0680031716.01	-0.831043	2.087112e-55	2.238480e-52
MsG0280007647.01	MsG0680031716.01	-0.826845	2.169435e-54	2.058314e-51
MsG0280008223.01	MsG0680031716.01	-0.810325	1.219521e-50	7.328970e-48
MsG0280008388.01	MsG0680031716.01	-0.843950	1.022437e-58	1.625700e-55
MsG0280008394.01	MsG0680031716.01	-0.848385	6.339581e-60	1.161383e-56
MsG0280008451.01	MsG0680031716.01	-0.851982	6.221670e-61	1.280998e-57
MsG0280008591.01	MsG0680031716.01	-0.822747	2.008068e-53	1.695691e-50
MsG0280009685.01	MsG0680031716.01	-0.809128	2.205336e-50	1.284180e-47
MsG0280010353.01	MsG0680031716.01	-0.825525	4.469799e-54	4.084148e-51
MsG0280010503.01	MsG0680031716.01	-0.841397	4.870231e-58	7.147566e-55

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031716.01.T01	MTR_6g033065	93.304	224	7	1	1	224	1	216	2.20e-158	441
MsG0680031716.01.T01	MTR_3g081150	59.799	199	70	2	24	220	22	212	4.10e-87	260
MsG0680031716.01.T01	MTR_1g063900	48.214	224	100	4	1	220	104	315	2.13e-73	229
MsG0680031716.01.T01	MTR_1g094650	53.922	204	84	3	18	220	146	340	1.87e-71	224
MsG0680031716.01.T01	MTR_1g063900	50.289	173	78	3	1	169	104	272	9.00e-56	181
MsG0680031716.01.T01	MTR_4g094860	36.957	92	56	1	77	166	28	119	1.88e-12	67.8
MsG0680031716.01.T01	MTR_4g094860	36.957	92	56	1	77	166	28	119	2.17e-12	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031716.01.T01	AT3G02070	75.622	201	41	1	24	224	13	205	1.36e-116	335
MsG0680031716.01.T01	AT3G02070	75.622	201	41	1	24	224	13	205	1.36e-116	335
MsG0680031716.01.T01	AT3G22260	60.714	196	68	2	26	220	36	223	4.73e-85	255
MsG0680031716.01.T01	AT3G22260	60.714	196	68	2	26	220	36	223	5.77e-85	255
MsG0680031716.01.T01	AT3G22260	60.714	196	68	2	26	220	36	223	5.77e-85	255
MsG0680031716.01.T01	AT3G22260	60.714	196	68	2	26	220	36	223	5.77e-85	255
MsG0680031716.01.T01	AT3G22260	60.714	196	68	2	26	220	36	223	5.77e-85	255
MsG0680031716.01.T01	AT3G22260	60.714	196	68	2	26	220	36	223	5.77e-85	255
MsG0680031716.01.T01	AT5G04250	51.813	193	82	2	28	220	145	326	5.97e-69	218
MsG0680031716.01.T01	AT5G04250	51.813	193	82	2	28	220	145	326	5.97e-69	218
MsG0680031716.01.T01	AT5G03330	46.256	227	110	3	4	229	129	344	1.38e-66	212
MsG0680031716.01.T01	AT5G03330	46.256	227	110	3	4	229	129	344	1.38e-66	212
MsG0680031716.01.T01	AT5G03330	45.673	208	101	3	4	210	129	325	9.34e-58	189
MsG0680031716.01.T01	AT5G03330	45.673	208	101	3	4	210	129	325	9.34e-58	189
MsG0680031716.01.T01	AT2G39320	33.594	128	57	3	95	221	2	102	1.86e-16	76.3
MsG0680031716.01.T01	AT5G67170	30.137	146	88	3	77	219	24	158	8.31e-12	65.5
MsG0680031716.01.T01	AT5G67170	30.137	146	88	3	77	219	24	158	8.31e-12	65.5

Find 76 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAAGTGTGGTTGAGCTTC+TGG	0.259325	6:-25236668	MsG0680031716.01.T01:CDS
CTTTGTGAAGTTAGAGTATC+TGG	0.260470	6:-25238209	MsG0680031716.01.T01:CDS
TGCAATTGTTGTAGGGTTAT+TGG	0.280794	6:-25240040	MsG0680031716.01.T01:intron
ACCAACTGCTCCATCTAAAT+TGG	0.295926	6:+25239832	None:intergenic
CTCCTTGGTTTGTGCTGGAT+TGG	0.304888	6:+25236521	None:intergenic
ATGGAAGACAGACTCAAAAT+TGG	0.312696	6:-25239949	MsG0680031716.01.T01:CDS
TTCGAACTTGGCACCTGTTC+CGG	0.338179	6:-25239801	MsG0680031716.01.T01:intron
GTTTCCTCCTTGGTTTGTGC+TGG	0.339042	6:+25236516	None:intergenic
CGAACATGCTTGTGGTGTTC+AGG	0.343767	6:+25238051	None:intergenic
TAATATCGTTTGTACTTCAT+TGG	0.355923	6:+25237550	None:intergenic
CACTATAAATGACGCCAGTA+TGG	0.372664	6:-25239196	MsG0680031716.01.T01:CDS
GGGTGACCATGTTACCTTAC+AGG	0.378745	6:-25237381	MsG0680031716.01.T01:CDS
TGGAAGACAGACTCAAAATT+GGG	0.391954	6:-25239948	MsG0680031716.01.T01:CDS
GTGTTGGGATTTAGAATGAA+TGG	0.391999	6:+25240896	None:intergenic
GAAGAGTATGCCAATTTAGA+TGG	0.397908	6:-25239842	MsG0680031716.01.T01:CDS
CTTACAGGCAGCTTCTGATA+AGG	0.408830	6:-25237366	MsG0680031716.01.T01:CDS
TCGTAAAGATATTGTAAAAC+AGG	0.423090	6:-25238031	MsG0680031716.01.T01:intron
TGGTCGGCGCCTTTCGAACT+TGG	0.423186	6:-25239813	MsG0680031716.01.T01:CDS
GCAGGGATTCCTACCGGAAC+AGG	0.425396	6:+25239788	None:intergenic
CATACTGGCGTCATTTATAG+TGG	0.427137	6:+25239197	None:intergenic
TCTTACCGCGCTGCGGTGCT+TGG	0.429756	6:+25237090	None:intergenic
AACAACCAATGTTTCCTCCT+TGG	0.432342	6:+25236506	None:intergenic
CTTATCAGAAGCTGCCTGTA+AGG	0.433796	6:+25237367	None:intergenic
TGTTGTAGGGTTATTGGCTG+TGG	0.439555	6:-25240034	MsG0680031716.01.T01:intron
TGGTACATAGGATTCATATA+AGG	0.449434	6:+25237570	None:intergenic
TTTGTACTTCATTGGTACAT+AGG	0.451108	6:+25237558	None:intergenic
AACTCACTTTATGAAATTCG+AGG	0.456383	6:-25236630	MsG0680031716.01.T01:CDS
CCTTGTGCAGATCTGGAGAA+TGG	0.471939	6:-25237403	MsG0680031716.01.T01:intron
ATCTGGAGATGGAAATTGTC+AGG	0.472072	6:-25238192	MsG0680031716.01.T01:intron
CACTTTCAGATCAGCTGTAC+CGG	0.478852	6:-25238077	MsG0680031716.01.T01:CDS
AGAAGATGTAGTGTCGTGTA+TGG	0.493008	6:-25236481	MsG0680031716.01.T01:CDS
AACCTGTGAGCGATTGTGTT+GGG	0.500150	6:+25240881	None:intergenic
CTTGTGCAGATCTGGAGAAT+GGG	0.504959	6:-25237402	MsG0680031716.01.T01:intron
AACTTCGCTTAGTAGTCAGC+AGG	0.513849	6:-25239903	MsG0680031716.01.T01:CDS
AATATGCTTATGCTTAATAG+AGG	0.514631	6:+25236585	None:intergenic
GTCATTTATAGTGGGGATGA+AGG	0.516277	6:+25239206	None:intergenic
ATTGGCTGTGGATTGTGTTG+AGG	0.516663	6:-25240022	MsG0680031716.01.T01:five_prime_UTR
GAAGAAGACACGGTAGTAGT+TGG	0.517300	6:+25241041	None:intergenic
GATGATTGCTCTTGTGCTAT+CGG	0.520346	6:-25239864	MsG0680031716.01.T01:CDS
TGTTGGGATTTAGAATGAAT+GGG	0.531457	6:+25240897	None:intergenic
CAAACGATATTACAAGAGAA+TGG	0.535116	6:-25237539	MsG0680031716.01.T01:CDS
CAGACGCTGATGATCCATAC+TGG	0.538309	6:+25239182	None:intergenic
GCCAATTTAGATGGAGCAGT+TGG	0.540811	6:-25239833	MsG0680031716.01.T01:CDS
GGGGATGAAGGTATTTATGC+GGG	0.542802	6:+25239218	None:intergenic
ATCATCAGCGTCTGCTACAG+AGG	0.553379	6:-25239173	MsG0680031716.01.T01:intron
CGCTGCGGTGCTTGGTACAG+TGG	0.555933	6:+25237098	None:intergenic
ATACTGGCGTCATTTATAGT+GGG	0.564488	6:+25239198	None:intergenic
GCTTATGCTTAATAGAGGAT+CGG	0.565678	6:+25236590	None:intergenic
GCTTGTGGTGTTCAGGTGAC+CGG	0.574916	6:+25238058	None:intergenic
GAACAGGTGCCAAGTTCGAA+AGG	0.581669	6:+25239804	None:intergenic
ATCCCAACACAATCGCTCAC+AGG	0.584837	6:-25240883	MsG0680031716.01.T01:intron
GAAGTTAGAGTATCTGGAGA+TGG	0.586909	6:-25238203	MsG0680031716.01.T01:CDS
GTGGTTGAGCTTCTGGTCCG+AGG	0.587217	6:-25236661	MsG0680031716.01.T01:CDS
ACAAACCAAGGAGGAAACAT+TGG	0.595884	6:-25236511	MsG0680031716.01.T01:CDS
ATTTAGATGGAGCAGTTGGT+CGG	0.596467	6:-25239829	MsG0680031716.01.T01:CDS
ATGAATGGGTGTGATGAAGA+AGG	0.597213	6:+25240911	None:intergenic
TATCTTTACGAACATGCTTG+TGG	0.599180	6:+25238043	None:intergenic
TATCTCTGAATGATGTCAGA+AGG	0.609488	6:+25237141	None:intergenic
AAGCTGCCTGTAAGGTAACA+TGG	0.614667	6:+25237375	None:intergenic
CACCAATCCAGCACAAACCA+AGG	0.617742	6:-25236523	MsG0680031716.01.T01:CDS
CATAGGATTCATATAAGGAA+CGG	0.626211	6:+25237575	None:intergenic
GTCGTGTATGGCTCATCGAA+TGG	0.630803	6:-25236469	MsG0680031716.01.T01:CDS
AAGTGAGTTGTAATGAACCT+CGG	0.634872	6:+25236644	None:intergenic
TACTGGCGTCATTTATAGTG+GGG	0.643650	6:+25239199	None:intergenic
AAACCTGTGAGCGATTGTGT+TGG	0.649827	6:+25240880	None:intergenic
ACAGCTGATCTGAAAGTGCA+CGG	0.650890	6:+25238082	None:intergenic
GGTGAGGTCTTACCGCGCTG+CGG	0.658969	6:+25237083	None:intergenic
GGAAGACAGACTCAAAATTG+GGG	0.661464	6:-25239947	MsG0680031716.01.T01:CDS
TGGGGATGAAGGTATTTATG+CGG	0.664042	6:+25239217	None:intergenic
TTCTTAATGATGCAGAAGTG+TGG	0.666124	6:-25236680	MsG0680031716.01.T01:intron
AATGGGTGTGATGAAGAAGG+AGG	0.677563	6:+25240914	None:intergenic
TTGTGCAGATCTGGAGAATG+GGG	0.683415	6:-25237401	MsG0680031716.01.T01:intron
CAATCCAGCACAAACCAAGG+AGG	0.692550	6:-25236520	MsG0680031716.01.T01:CDS
AAGGTTGATGGAAGAAGACA+CGG	0.696191	6:+25241031	None:intergenic
CTGTACCAAGCACCGCAGCG+CGG	0.717604	6:-25237095	MsG0680031716.01.T01:intron
GGGATGAAGGTATTTATGCG+GGG	0.722601	6:+25239219	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATGTAAATAGTTAATAAT+TGG	+	Chr6:25240168-25240187	None:intergenic	10.0%
!!	ATATAAATTAATACATTTCA+CGG	+	Chr6:25239502-25239521	None:intergenic	10.0%
!!	ATTAAAAAAAATATGAAGAA+AGG	+	Chr6:25236627-25236646	None:intergenic	10.0%
!!	TTAAAAAAAATATGAAGAAA+GGG	+	Chr6:25236626-25236645	None:intergenic	10.0%
!!!	AGAAAATTTAAAACTATATT+AGG	+	Chr6:25239342-25239361	None:intergenic	10.0%
!!!	TTTTTACAAGAAAAATATTT+TGG	-	Chr6:25237000-25237019	MsG0680031716.01.T01:intron	10.0%
!!	AGTTATATACTGTAATATTT+AGG	-	Chr6:25239708-25239727	MsG0680031716.01.T01:intron	15.0%
!!	GTTATATACTGTAATATTTA+GGG	-	Chr6:25239709-25239728	MsG0680031716.01.T01:intron	15.0%
!!	TACATTAAAAAATAAGAAAC+AGG	+	Chr6:25240289-25240308	None:intergenic	15.0%
!!	TATAAATTAATACATTTCAC+GGG	+	Chr6:25239501-25239520	None:intergenic	15.0%
!!	TTTATAATCTTGAATTTGAA+GGG	-	Chr6:25236891-25236910	MsG0680031716.01.T01:intron	15.0%
!!!	AAATTTTATTGTTAAACTGT+GGG	+	Chr6:25237724-25237743	None:intergenic	15.0%
!!!	TAAAAGTAAAGTTTTCAATT+TGG	+	Chr6:25236474-25236493	None:intergenic	15.0%
!!	AAGAAAATAAAAGGAAACAA+AGG	+	Chr6:25240773-25240792	None:intergenic	20.0%
!!	ATATATAATAGCTTAAGTGA+CGG	+	Chr6:25239978-25239997	None:intergenic	20.0%
!!	ATATTTAGGGAAGAAAAATA+CGG	-	Chr6:25239722-25239741	MsG0680031716.01.T01:intron	20.0%
!!	CTTTATAATCTTGAATTTGA+AGG	-	Chr6:25236890-25236909	MsG0680031716.01.T01:intron	20.0%
!!	TACAACTATATCTACAATAA+TGG	+	Chr6:25238788-25238807	None:intergenic	20.0%
!!	TGCATCATTAAGAAAATAAA+AGG	+	Chr6:25240782-25240801	None:intergenic	20.0%
!!!	AATTTTTATTTAGAATAGCG+AGG	+	Chr6:25237899-25237918	None:intergenic	20.0%
!!!	ACAAGAAAAATATTTTGGAT+TGG	-	Chr6:25237005-25237024	MsG0680031716.01.T01:intron	20.0%
!!!	ATCATTTTCTATCATTGATT+TGG	-	Chr6:25236783-25236802	MsG0680031716.01.T01:intron	20.0%
!!!	CAAATTTTATTGTTAAACTG+TGG	+	Chr6:25237725-25237744	None:intergenic	20.0%
!!!	CAAGAAAAATATTTTGGATT+GGG	-	Chr6:25237006-25237025	MsG0680031716.01.T01:intron	20.0%
!!!	CCATTTTCTGAAATTATTAA+CGG	-	Chr6:25237125-25237144	MsG0680031716.01.T01:CDS	20.0%
!!!	GAATTTTACGATTAATAATG+TGG	-	Chr6:25237335-25237354	MsG0680031716.01.T01:intron	20.0%
!!!	GATTGTACTATTAGAATTTT+AGG	-	Chr6:25236949-25236968	MsG0680031716.01.T01:intron	20.0%
!!!	TATCCATTTTCAATATTCAA+CGG	+	Chr6:25239821-25239840	None:intergenic	20.0%
!!!	TATGGAATTTTCAATATTTG+TGG	-	Chr6:25238178-25238197	MsG0680031716.01.T01:intron	20.0%
!	AAAAAATATGAAGAAAGGGA+AGG	+	Chr6:25236622-25236641	None:intergenic	25.0%
!	AACGATAAAGAACAAATTTC+AGG	+	Chr6:25239606-25239625	None:intergenic	25.0%
!	AAGAATTTCAGTAACTTATC+CGG	+	Chr6:25239012-25239031	None:intergenic	25.0%
!	AATATGCTTATGCTTAATAG+AGG	+	Chr6:25240886-25240905	None:intergenic	25.0%
!	ATAAGTTACTGAAATTCTTC+TGG	-	Chr6:25239013-25239032	MsG0680031716.01.T01:intron	25.0%
!	ATGAACAAAAAAGATCGAAT+CGG	+	Chr6:25237398-25237417	None:intergenic	25.0%
!	ATGTTTATGCAATTGTTGTA+GGG	-	Chr6:25237421-25237440	MsG0680031716.01.T01:intron	25.0%
!	CCGTTAATAATTTCAGAAAA+TGG	+	Chr6:25237128-25237147	None:intergenic	25.0%
!	CTAAAAGAGTGAAGATAAAA+TGG	+	Chr6:25239223-25239242	None:intergenic	25.0%
!	GAATTCTCTTGACAAAATTA+AGG	-	Chr6:25240196-25240215	MsG0680031716.01.T01:intron	25.0%
!	GTAAAATTCTAATAACCATG+AGG	+	Chr6:25237324-25237343	None:intergenic	25.0%
!	GTACAAATTATTCTGAATAG+AGG	-	Chr6:25238605-25238624	MsG0680031716.01.T01:intron	25.0%
!	TAATATCGTTTGTACTTCAT+TGG	+	Chr6:25239921-25239940	None:intergenic	25.0%
!	TATGTTTATGCAATTGTTGT+AGG	-	Chr6:25237420-25237439	MsG0680031716.01.T01:intron	25.0%
!	TCGTAAAGATATTGTAAAAC+AGG	-	Chr6:25239437-25239456	MsG0680031716.01.T01:intron	25.0%
!	TGGATTATTTAATGATGTAG+TGG	-	Chr6:25239050-25239069	MsG0680031716.01.T01:intron	25.0%
!	TTCAATGTAATCACATTTGT+CGG	+	Chr6:25237100-25237119	None:intergenic	25.0%
!	TTTGTGAGTAAAAGTCATTT+CGG	-	Chr6:25240553-25240572	MsG0680031716.01.T01:intron	25.0%
!!	AATGACTTTTACTCACAAAT+AGG	+	Chr6:25240552-25240571	None:intergenic	25.0%
!!	AATTTTGCAACAAGATTAGT+TGG	-	Chr6:25237920-25237939	MsG0680031716.01.T01:intron	25.0%
!!	ATCCATTTTCAATATTCAAC+GGG	+	Chr6:25239820-25239839	None:intergenic	25.0%
!!	ATGACTTTTACTCACAAATA+GGG	+	Chr6:25240551-25240570	None:intergenic	25.0%
!!	ATGGTTAACGAAGTATTATA+TGG	-	Chr6:25238959-25238978	MsG0680031716.01.T01:intron	25.0%
!!	CATTTTATCTTCACTCTTTT+AGG	-	Chr6:25239221-25239240	MsG0680031716.01.T01:CDS	25.0%
!!	GTAGATATAGTTGTACTTTT+GGG	-	Chr6:25238793-25238812	MsG0680031716.01.T01:intron	25.0%
!!	TCGAAGTTTCAAAAAGTTTT+CGG	-	Chr6:25237481-25237500	MsG0680031716.01.T01:intron	25.0%
!!	TGGTTAACGAAGTATTATAT+GGG	-	Chr6:25238960-25238979	MsG0680031716.01.T01:intron	25.0%
!!	TGTAGATATAGTTGTACTTT+TGG	-	Chr6:25238792-25238811	MsG0680031716.01.T01:intron	25.0%
!!	TTTATCTCCAATCTTTTCTT+TGG	+	Chr6:25239557-25239576	None:intergenic	25.0%
!!	TTTCAAAAAGTTTTCGGTTT+TGG	-	Chr6:25237487-25237506	MsG0680031716.01.T01:intron	25.0%
!!	TTTTATTGCAATTGTGTACA+AGG	-	Chr6:25239304-25239323	MsG0680031716.01.T01:intron	25.0%
!!!	AGTTTTCAATTTGGTGTTTT+TGG	+	Chr6:25236465-25236484	None:intergenic	25.0%
!!!	ATTACCAGTGTTGTTAATTT+TGG	-	Chr6:25238518-25238537	MsG0680031716.01.T01:intron	25.0%
!!!	CCTAGATTTTTAAAATAACG+AGG	-	Chr6:25239128-25239147	MsG0680031716.01.T01:intron	25.0%
!!!	CCTCGTTATTTTAAAAATCT+AGG	+	Chr6:25239131-25239150	None:intergenic	25.0%
!!!	GTAATATGTGCTATTTGAAA+TGG	+	Chr6:25238503-25238522	None:intergenic	25.0%
!!!	TTACCAGTGTTGTTAATTTT+GGG	-	Chr6:25238519-25238538	MsG0680031716.01.T01:intron	25.0%
!!!	TTTTAGTTCTTAAATGTGTG+TGG	+	Chr6:25240705-25240724	None:intergenic	25.0%
	AACTCACTTTATGAAATTCG+AGG	-	Chr6:25240838-25240857	MsG0680031716.01.T01:intron	30.0%
	AAGCAAGTAACTCAAAGTTA+AGG	-	Chr6:25239093-25239112	MsG0680031716.01.T01:intron	30.0%
	AATATGAGACGGTTGTAAAA+TGG	+	Chr6:25237242-25237261	None:intergenic	30.0%
	AATTATTATCTCTGTAGCTC+CGG	-	Chr6:25237030-25237049	MsG0680031716.01.T01:intron	30.0%
	ATATGCACTGTTATCAATTG+CGG	+	Chr6:25238693-25238712	None:intergenic	30.0%
	CAAACGATATTACAAGAGAA+TGG	-	Chr6:25239929-25239948	MsG0680031716.01.T01:CDS	30.0%
	CATAGGATTCATATAAGGAA+CGG	+	Chr6:25239896-25239915	None:intergenic	30.0%
	CTAAGGTTACTATTTACTAC+AGG	+	Chr6:25239640-25239659	None:intergenic	30.0%
	GCATATTCCAAAGAAAAGAT+TGG	-	Chr6:25239547-25239566	MsG0680031716.01.T01:intron	30.0%
	GGAAAAACTTATCACTTGTT+AGG	-	Chr6:25237356-25237375	MsG0680031716.01.T01:CDS	30.0%
	GTAGATTCTACATAACAGAA+TGG	+	Chr6:25238325-25238344	None:intergenic	30.0%
	TAAATACGTCTTTCGTTAGT+CGG	+	Chr6:25240635-25240654	None:intergenic	30.0%
	TATGAAAATGACTTGAGGAT+AGG	-	Chr6:25238727-25238746	MsG0680031716.01.T01:intron	30.0%
	TGATAACAGTGCATATAACT+TGG	-	Chr6:25238697-25238716	MsG0680031716.01.T01:intron	30.0%
	TGGTACATAGGATTCATATA+AGG	+	Chr6:25239901-25239920	None:intergenic	30.0%
	TGTTCAAATCCAATATGAGA+CGG	+	Chr6:25237253-25237272	None:intergenic	30.0%
	TTGTTCTTTATCGTTGATCA+AGG	-	Chr6:25239611-25239630	MsG0680031716.01.T01:intron	30.0%
	TTTGTACTTCATTGGTACAT+AGG	+	Chr6:25239913-25239932	None:intergenic	30.0%
	TTTGTCAAGAGAATTCGAAA+AGG	+	Chr6:25240192-25240211	None:intergenic	30.0%
!	AGAATCTACTATTTCTTGTG+AGG	-	Chr6:25238336-25238355	MsG0680031716.01.T01:intron	30.0%
!	ATTTTACAACCGTCTCATAT+TGG	-	Chr6:25237241-25237260	MsG0680031716.01.T01:intron	30.0%
!	TAACTTTGAGTTACTTGCTT+AGG	+	Chr6:25239093-25239112	None:intergenic	30.0%
!	TAGCTGTATTTCTACCAAAA+GGG	+	Chr6:25238393-25238412	None:intergenic	30.0%
!	TCCATTTTCAATATTCAACG+GGG	+	Chr6:25239819-25239838	None:intergenic	30.0%
!	TCTTCTTTTTAAATCACTCG+CGG	-	Chr6:25237787-25237806	MsG0680031716.01.T01:intron	30.0%
!	TGTTGGGATTTAGAATGAAT+GGG	+	Chr6:25236574-25236593	None:intergenic	30.0%
!	TTCTGGTTACAATAAGTACT+TGG	-	Chr6:25239030-25239049	MsG0680031716.01.T01:intron	30.0%
!	TTTCAATTTTCTTCTCTAGC+AGG	+	Chr6:25239674-25239693	None:intergenic	30.0%
!	TTTTATTGTTAAACTGTGGG+TGG	+	Chr6:25237721-25237740	None:intergenic	30.0%
!!	GTTTTGAATCAGGTTTTTGT+TGG	-	Chr6:25240136-25240155	MsG0680031716.01.T01:intron	30.0%
!!	TACCAGTGTTGTTAATTTTG+GGG	-	Chr6:25238520-25238539	MsG0680031716.01.T01:intron	30.0%
!!	TTATCTTCACTCTTTTAGGT+TGG	-	Chr6:25239225-25239244	MsG0680031716.01.T01:CDS	30.0%
!!	TTGTGTTGAGGTTTTTGTTT+AGG	-	Chr6:25237458-25237477	MsG0680031716.01.T01:intron	30.0%
!!!	CAATTTGGTGTTTTTGGTTA+AGG	+	Chr6:25236459-25236478	None:intergenic	30.0%
!!!	TCGTAATGTTGTTTTGAATC+AGG	-	Chr6:25240126-25240145	MsG0680031716.01.T01:intron	30.0%
!!!	TCTTTTAGGTTGGATGTTTA+TGG	-	Chr6:25239235-25239254	MsG0680031716.01.T01:intron	30.0%
	AAACTCTTTCCTACACTAGA+AGG	+	Chr6:25238762-25238781	None:intergenic	35.0%
	AACTCTTTGTACAAGAAGAG+TGG	-	Chr6:25239773-25239792	MsG0680031716.01.T01:intron	35.0%
	AAGTGAGTTGTAATGAACCT+CGG	+	Chr6:25240827-25240846	None:intergenic	35.0%
	ACCATTAACCGATCTTAAGA+GGG	-	Chr6:25238054-25238073	MsG0680031716.01.T01:CDS	35.0%
	ACCCCGTTGAATATTGAAAA+TGG	-	Chr6:25239815-25239834	MsG0680031716.01.T01:CDS	35.0%
	ACGGTGTTTAAATCATGTGT+GGG	-	Chr6:25239741-25239760	MsG0680031716.01.T01:intron	35.0%
	ACTGTTATCAATTGCGGATT+GGG	+	Chr6:25238687-25238706	None:intergenic	35.0%
	ATACTGGCGTCATTTATAGT+GGG	+	Chr6:25238273-25238292	None:intergenic	35.0%
	ATGGAAGACAGACTCAAAAT+TGG	-	Chr6:25237519-25237538	MsG0680031716.01.T01:intron	35.0%
	ATTCCTATGTAAGATCACTC+GGG	+	Chr6:25237964-25237983	None:intergenic	35.0%
	ATTCTGAATAGAGGCTATAG+CGG	-	Chr6:25238614-25238633	MsG0680031716.01.T01:intron	35.0%
	ATTTGCCCCAAAAGTCATTT+CGG	-	Chr6:25240466-25240485	MsG0680031716.01.T01:intron	35.0%
	CACATAATTCCTTCTAGTGT+AGG	-	Chr6:25238750-25238769	MsG0680031716.01.T01:intron	35.0%
	CTACTAATAGTAATGACCAC+GGG	-	Chr6:25238410-25238429	MsG0680031716.01.T01:intron	35.0%
	CTCAAGGAATAAGCAAAGAT+AGG	-	Chr6:25238905-25238924	MsG0680031716.01.T01:intron	35.0%
	CTTCGTATGAAAATGACTTG+AGG	-	Chr6:25238722-25238741	MsG0680031716.01.T01:intron	35.0%
	CTTGATGCAAATGCTTCTAA+AGG	+	Chr6:25240027-25240046	None:intergenic	35.0%
	GATTCCTATGTAAGATCACT+CGG	+	Chr6:25237965-25237984	None:intergenic	35.0%
	GCTACTAATAGTAATGACCA+CGG	-	Chr6:25238409-25238428	MsG0680031716.01.T01:intron	35.0%
	GCTGTGGATTTACTCTTTAT+CGG	-	Chr6:25239188-25239207	MsG0680031716.01.T01:CDS	35.0%
	GCTTATGCTTAATAGAGGAT+CGG	+	Chr6:25240881-25240900	None:intergenic	35.0%
	TAACTTATCCGGATACAGAA+AGG	+	Chr6:25239001-25239020	None:intergenic	35.0%
	TACGGTGTTTAAATCATGTG+TGG	-	Chr6:25239740-25239759	MsG0680031716.01.T01:intron	35.0%
	TATCTCTGAATGATGTCAGA+AGG	+	Chr6:25240330-25240349	None:intergenic	35.0%
	TATCTTTACGAACATGCTTG+TGG	+	Chr6:25239428-25239447	None:intergenic	35.0%
	TCATATAACTACTCTTGCTG+TGG	-	Chr6:25239172-25239191	MsG0680031716.01.T01:intron	35.0%
	TCTAGTGTAGGAAAGAGTTT+GGG	-	Chr6:25238762-25238781	MsG0680031716.01.T01:intron	35.0%
	TCTTAAGATCGGTTAATGGT+GGG	+	Chr6:25238054-25238073	None:intergenic	35.0%
	TGCAATTGTTGTAGGGTTAT+TGG	-	Chr6:25237428-25237447	MsG0680031716.01.T01:intron	35.0%
	TGGAAGACAGACTCAAAATT+GGG	-	Chr6:25237520-25237539	MsG0680031716.01.T01:intron	35.0%
	TTCTAGTGTAGGAAAGAGTT+TGG	-	Chr6:25238761-25238780	MsG0680031716.01.T01:intron	35.0%
	TTCTTAATGATGCAGAAGTG+TGG	-	Chr6:25240788-25240807	MsG0680031716.01.T01:intron	35.0%
	TTTAAATCATGTGTGGGATG+TGG	-	Chr6:25239747-25239766	MsG0680031716.01.T01:intron	35.0%
	TTTCGGTCTCTAATTGTATG+TGG	-	Chr6:25240570-25240589	MsG0680031716.01.T01:intron	35.0%
!	CTTTGTGAAGTTAGAGTATC+TGG	-	Chr6:25239259-25239278	MsG0680031716.01.T01:intron	35.0%
!	GAAGAGTATGCCAATTTAGA+TGG	-	Chr6:25237626-25237645	MsG0680031716.01.T01:intron	35.0%
!	GTAGCTGTATTTCTACCAAA+AGG	+	Chr6:25238394-25238413	None:intergenic	35.0%
!	GTGTTGGGATTTAGAATGAA+TGG	+	Chr6:25236575-25236594	None:intergenic	35.0%
!	GTTCTCGTCGAAAGTTTTTA+TGG	+	Chr6:25238863-25238882	None:intergenic	35.0%
!	TTATTTAGAATAGCGAGGCA+AGG	+	Chr6:25237894-25237913	None:intergenic	35.0%
!!	AAAGGTATCGAAAAATGCGA+TGG	+	Chr6:25240009-25240028	None:intergenic	35.0%
!!	ATAGTTGTACTTTTGGGAGA+CGG	-	Chr6:25238799-25238818	MsG0680031716.01.T01:intron	35.0%
!!	TAATAGAGGATCGGTTTTAG+TGG	+	Chr6:25240872-25240891	None:intergenic	35.0%
!!!	GTGTTTTTGGTTAAGGTTGA+TGG	+	Chr6:25236452-25236471	None:intergenic	35.0%
!!!	TCGGTTTTGGATATGATGAA+TGG	-	Chr6:25237500-25237519	MsG0680031716.01.T01:intron	35.0%
	AACAACCAATGTTTCCTCCT+TGG	+	Chr6:25240965-25240984	None:intergenic	40.0%
	ACAAACCAAGGAGGAAACAT+TGG	-	Chr6:25240957-25240976	MsG0680031716.01.T01:five_prime_UTR	40.0%
	ACCAACTGCTCCATCTAAAT+TGG	+	Chr6:25237639-25237658	None:intergenic	40.0%
	AGAAGATGTAGTGTCGTGTA+TGG	-	Chr6:25240987-25241006	MsG0680031716.01.T01:five_prime_UTR	40.0%
	AGCAACAGTTCATTCGATGA+GGG	+	Chr6:25240493-25240512	None:intergenic	40.0%
	AGCCCCAAAATTAACAACAC+TGG	+	Chr6:25238525-25238544	None:intergenic	40.0%
	ATCTGGAGATGGAAATTGTC+AGG	-	Chr6:25239276-25239295	MsG0680031716.01.T01:intron	40.0%
	ATGAATGGGTGTGATGAAGA+AGG	+	Chr6:25236560-25236579	None:intergenic	40.0%
	ATGTGCTTTAACCCGATAGT+AGG	-	Chr6:25238005-25238024	MsG0680031716.01.T01:intron	40.0%
	ATTTAGATGGAGCAGTTGGT+CGG	-	Chr6:25237639-25237658	MsG0680031716.01.T01:intron	40.0%
	CACCATTAACCGATCTTAAG+AGG	-	Chr6:25238053-25238072	MsG0680031716.01.T01:CDS	40.0%
	CACTATAAATGACGCCAGTA+TGG	-	Chr6:25238272-25238291	MsG0680031716.01.T01:intron	40.0%
	CACTGTTATCAATTGCGGAT+TGG	+	Chr6:25238688-25238707	None:intergenic	40.0%
	CAGGTAAGTTGTGCTTATCT+TGG	-	Chr6:25239456-25239475	MsG0680031716.01.T01:intron	40.0%
	CATACTGGCGTCATTTATAG+TGG	+	Chr6:25238274-25238293	None:intergenic	40.0%
	CATTTCTCTTACGACTTGTG+AGG	+	Chr6:25238942-25238961	None:intergenic	40.0%
	CTCACAAGTCGTAAGAGAAA+TGG	-	Chr6:25238940-25238959	MsG0680031716.01.T01:intron	40.0%
	CTCTTAAGATCGGTTAATGG+TGG	+	Chr6:25238055-25238074	None:intergenic	40.0%
	CTTAAGATCGGTTAATGGTG+GGG	+	Chr6:25238053-25238072	None:intergenic	40.0%
	CTTATCTTGGTTGTGTGCAA+TGG	-	Chr6:25239469-25239488	MsG0680031716.01.T01:intron	40.0%
	GATGATTGCTCTTGTGCTAT+CGG	-	Chr6:25237604-25237623	MsG0680031716.01.T01:intron	40.0%
	GATTTGAACAATGTCCGTTG+AGG	-	Chr6:25237263-25237282	MsG0680031716.01.T01:intron	40.0%
	GCTATAGCACTGTTCTATAG+CGG	-	Chr6:25238638-25238657	MsG0680031716.01.T01:intron	40.0%
	GGAAGACAGACTCAAAATTG+GGG	-	Chr6:25237521-25237540	MsG0680031716.01.T01:intron	40.0%
	GGAGCATAACAGAAAAAGCA+GGG	+	Chr6:25237700-25237719	None:intergenic	40.0%
	TACTGGCGTCATTTATAGTG+GGG	+	Chr6:25238272-25238291	None:intergenic	40.0%
	TGCGAGTATTCACCCAAAAA+GGG	+	Chr6:25238126-25238145	None:intergenic	40.0%
	TGGAGCATAACAGAAAAAGC+AGG	+	Chr6:25237701-25237720	None:intergenic	40.0%
	TGTATGGTCATCCCTACTAT+CGG	+	Chr6:25238020-25238039	None:intergenic	40.0%
	TGTGCTTTAACCCGATAGTA+GGG	-	Chr6:25238006-25238025	MsG0680031716.01.T01:intron	40.0%
!	AGTTATCGTCTATGCCCTTT+TGG	-	Chr6:25238376-25238395	MsG0680031716.01.T01:intron	40.0%
!	CTGTTATCAATTGCGGATTG+GGG	+	Chr6:25238686-25238705	None:intergenic	40.0%
!	GAAGTTAGAGTATCTGGAGA+TGG	-	Chr6:25239265-25239284	MsG0680031716.01.T01:intron	40.0%
!	GTCATTTATAGTGGGGATGA+AGG	+	Chr6:25238265-25238284	None:intergenic	40.0%
!	GTTTGACTTTGTCACCTCAA+CGG	+	Chr6:25237280-25237299	None:intergenic	40.0%
!!	AAGGTTGATGGAAGAAGACA+CGG	+	Chr6:25236440-25236459	None:intergenic	40.0%
!!	CACATCCCTTTTCTGAAAAG+GGG	-	Chr6:25238144-25238163	MsG0680031716.01.T01:intron	40.0%
!!	GCACATCCCTTTTCTGAAAA+GGG	-	Chr6:25238143-25238162	MsG0680031716.01.T01:intron	40.0%
!!	TGGGGATGAAGGTATTTATG+CGG	+	Chr6:25238254-25238273	None:intergenic	40.0%
	AAACCTGTGAGCGATTGTGT+TGG	+	Chr6:25236591-25236610	None:intergenic	45.0%
	AAACTACGAAGCACGTGCAT+TGG	+	Chr6:25237190-25237209	None:intergenic	45.0%
	AACAGCGACCTTTCTGTATC+CGG	-	Chr6:25238990-25239009	MsG0680031716.01.T01:intron	45.0%
	AACCTGTGAGCGATTGTGTT+GGG	+	Chr6:25236590-25236609	None:intergenic	45.0%
	AACTTCGCTTAGTAGTCAGC+AGG	-	Chr6:25237565-25237584	MsG0680031716.01.T01:CDS	45.0%
	AAGCTGCCTGTAAGGTAACA+TGG	+	Chr6:25240096-25240115	None:intergenic	45.0%
	AAGTGCATCATTTCTTCCCG+TGG	+	Chr6:25238429-25238448	None:intergenic	45.0%
	AATGGGTGTGATGAAGAAGG+AGG	+	Chr6:25236557-25236576	None:intergenic	45.0%
	AGGAACGGTGATCTTTAAGC+TGG	+	Chr6:25239881-25239900	None:intergenic	45.0%
	CACTTTCAGATCAGCTGTAC+CGG	-	Chr6:25239391-25239410	MsG0680031716.01.T01:intron	45.0%
	CAGCAACAGTTCATTCGATG+AGG	+	Chr6:25240494-25240513	None:intergenic	45.0%
	CTCAACCCTATTGCTAGAGT+TGG	+	Chr6:25238090-25238109	None:intergenic	45.0%
	CTGGCATTCTTCAAAGCTCA+AGG	-	Chr6:25238889-25238908	MsG0680031716.01.T01:intron	45.0%
	CTTACAGGCAGCTTCTGATA+AGG	-	Chr6:25240102-25240121	MsG0680031716.01.T01:intron	45.0%
	CTTATCAGAAGCTGCCTGTA+AGG	+	Chr6:25240104-25240123	None:intergenic	45.0%
	CTTGTGCAGATCTGGAGAAT+GGG	-	Chr6:25240066-25240085	MsG0680031716.01.T01:intron	45.0%
	GAAAAAGCAGGGATTCCTAC+CGG	+	Chr6:25237689-25237708	None:intergenic	45.0%
	GAAGAAGACACGGTAGTAGT+TGG	+	Chr6:25236430-25236449	None:intergenic	45.0%
	GCAACAGTTCATTCGATGAG+GGG	+	Chr6:25240492-25240511	None:intergenic	45.0%
	GCCAATTTAGATGGAGCAGT+TGG	-	Chr6:25237635-25237654	MsG0680031716.01.T01:intron	45.0%
	GCCCTCTTAAGATCGGTTAA+TGG	+	Chr6:25238058-25238077	None:intergenic	45.0%
	GCGAGTATTCACCCAAAAAG+GGG	+	Chr6:25238125-25238144	None:intergenic	45.0%
	GCTAGCCAACTCTAGCAATA+GGG	-	Chr6:25238082-25238101	MsG0680031716.01.T01:CDS	45.0%
	GGGCATAGACGATAACTTCA+AGG	+	Chr6:25238373-25238392	None:intergenic	45.0%
	GTATGGTCATCCCTACTATC+GGG	+	Chr6:25238019-25238038	None:intergenic	45.0%
	GTGCGAGTATTCACCCAAAA+AGG	+	Chr6:25238127-25238146	None:intergenic	45.0%
	TAGCCCGAGTGATCTTACAT+AGG	-	Chr6:25237958-25237977	MsG0680031716.01.T01:intron	45.0%
	TATTCACCCAAAAAGGGGCA+GGG	+	Chr6:25238120-25238139	None:intergenic	45.0%
	TGCTAGCCAACTCTAGCAAT+AGG	-	Chr6:25238081-25238100	MsG0680031716.01.T01:CDS	45.0%
	TTGTGCAGATCTGGAGAATG+GGG	-	Chr6:25240067-25240086	MsG0680031716.01.T01:intron	45.0%
	TTTCGACGAGAACAGCATAC+TGG	-	Chr6:25238870-25238889	MsG0680031716.01.T01:intron	45.0%
!	ACAGCTGATCTGAAAGTGCA+CGG	+	Chr6:25239389-25239408	None:intergenic	45.0%
!	ATTGGCTGTGGATTGTGTTG+AGG	-	Chr6:25237446-25237465	MsG0680031716.01.T01:intron	45.0%
!	TCCCACCCCTTTTCAGAAAA+GGG	+	Chr6:25238152-25238171	None:intergenic	45.0%
!	TGTTGTAGGGTTATTGGCTG+TGG	-	Chr6:25237434-25237453	MsG0680031716.01.T01:intron	45.0%
!!	ATCCCTTTTCTGAAAAGGGG+TGG	-	Chr6:25238147-25238166	MsG0680031716.01.T01:intron	45.0%
!!	CGCACATCCCTTTTCTGAAA+AGG	-	Chr6:25238142-25238161	MsG0680031716.01.T01:intron	45.0%
!!	GGGATGAAGGTATTTATGCG+GGG	+	Chr6:25238252-25238271	None:intergenic	45.0%
!!	GGGGATGAAGGTATTTATGC+GGG	+	Chr6:25238253-25238272	None:intergenic	45.0%
!!	TCCCTTTTCTGAAAAGGGGT+GGG	-	Chr6:25238148-25238167	MsG0680031716.01.T01:intron	45.0%
!!	TTGCGGATTGGGGAAAATAG+CGG	+	Chr6:25238676-25238695	None:intergenic	45.0%
!!	ATAAAAAATAAAAAAAATGA+TGG	+	Chr6:25236714-25236733	None:intergenic	5.0%
!!	TAAAAAATAAAAAAAATGAT+GGG	+	Chr6:25236713-25236732	None:intergenic	5.0%
	AGCAGGACGTCTTTGCTCTA+AGG	+	Chr6:25239657-25239676	None:intergenic	50.0%
	ATCATCAGCGTCTGCTACAG+AGG	-	Chr6:25238295-25238314	MsG0680031716.01.T01:intron	50.0%
	ATCCCAACACAATCGCTCAC+AGG	-	Chr6:25236585-25236604	MsG0680031716.01.T01:CDS	50.0%
	CAATCCAGCACAAACCAAGG+AGG	-	Chr6:25240948-25240967	MsG0680031716.01.T01:five_prime_UTR	50.0%
	CACCAATCCAGCACAAACCA+AGG	-	Chr6:25240945-25240964	MsG0680031716.01.T01:five_prime_UTR	50.0%
	CAGACGCTGATGATCCATAC+TGG	+	Chr6:25238289-25238308	None:intergenic	50.0%
	CCATTCTCCAGATCTGCACA+AGG	+	Chr6:25240068-25240087	None:intergenic	50.0%
	CCTTGTGCAGATCTGGAGAA+TGG	-	Chr6:25240065-25240084	MsG0680031716.01.T01:intron	50.0%
	CGAACATGCTTGTGGTGTTC+AGG	+	Chr6:25239420-25239439	None:intergenic	50.0%
	GAACAGGTGCCAAGTTCGAA+AGG	+	Chr6:25237667-25237686	None:intergenic	50.0%
	GATCACTCGGGCTAAAAGTG+CGG	+	Chr6:25237952-25237971	None:intergenic	50.0%
	GGGTGACCATGTTACCTTAC+AGG	-	Chr6:25240087-25240106	MsG0680031716.01.T01:intron	50.0%
	GTATTCACCCAAAAAGGGGC+AGG	+	Chr6:25238121-25238140	None:intergenic	50.0%
	GTCGTGTATGGCTCATCGAA+TGG	-	Chr6:25240999-25241018	MsG0680031716.01.T01:five_prime_UTR	50.0%
	TACTGAGCTGACACACCTCA+TGG	-	Chr6:25237306-25237325	MsG0680031716.01.T01:intron	50.0%
	TCTGACATGCTCACACACGT+TGG	+	Chr6:25237075-25237094	None:intergenic	50.0%
	TCTGCACAAGGTCGAAGAGA+AGG	+	Chr6:25240056-25240075	None:intergenic	50.0%
	TCTTCGACCTTGTGCAGATC+TGG	-	Chr6:25240058-25240077	MsG0680031716.01.T01:intron	50.0%
	TGCGCTTTCATGTAAGCTCG+TGG	-	Chr6:25237855-25237874	MsG0680031716.01.T01:intron	50.0%
	TTCGAACTTGGCACCTGTTC+CGG	-	Chr6:25237667-25237686	MsG0680031716.01.T01:intron	50.0%
	TTGCTAGAGTTGGCTAGCAC+TGG	+	Chr6:25238080-25238099	None:intergenic	50.0%
!	AAAGGGGTGGGAGGAGTATA+TGG	-	Chr6:25238160-25238179	MsG0680031716.01.T01:intron	50.0%
!	AAGATCGGTTAATGGTGGGG+CGG	+	Chr6:25238050-25238069	None:intergenic	50.0%
!	AATCTCACCCTGCCCCTTTT+TGG	-	Chr6:25238110-25238129	MsG0680031716.01.T01:intron	50.0%
!	AGGGGCCGAAATGACTTTTG+GGG	+	Chr6:25240474-25240493	None:intergenic	50.0%
!	ATCTCACCCTGCCCCTTTTT+GGG	-	Chr6:25238111-25238130	MsG0680031716.01.T01:intron	50.0%
!	CAGAAGTGTGGTTGAGCTTC+TGG	-	Chr6:25240800-25240819	MsG0680031716.01.T01:intron	50.0%
!	CTCCCACCCCTTTTCAGAAA+AGG	+	Chr6:25238153-25238172	None:intergenic	50.0%
!	GAAAGTGCACGGAACTGCAA+CGG	+	Chr6:25239378-25239397	None:intergenic	50.0%
!	GAGGGGCCGAAATGACTTTT+GGG	+	Chr6:25240475-25240494	None:intergenic	50.0%
!	TGAGGGGCCGAAATGACTTT+TGG	+	Chr6:25240476-25240495	None:intergenic	50.0%
!!	AGCACTGGCCCTCTTAAGAT+CGG	+	Chr6:25238065-25238084	None:intergenic	50.0%
!!	CTCCTTGGTTTGTGCTGGAT+TGG	+	Chr6:25240950-25240969	None:intergenic	50.0%
!!	CTTTTCTGAAAAGGGGTGGG+AGG	-	Chr6:25238151-25238170	MsG0680031716.01.T01:intron	50.0%
!!	GTTTCCTCCTTGGTTTGTGC+TGG	+	Chr6:25240955-25240974	None:intergenic	50.0%
	AACTTGGCACCTGTTCCGGT+AGG	-	Chr6:25237671-25237690	MsG0680031716.01.T01:intron	55.0%
	GCTTGTGGTGTTCAGGTGAC+CGG	+	Chr6:25239413-25239432	None:intergenic	55.0%
	GTGGGGCGGGATAAACTGTA+TGG	+	Chr6:25238036-25238055	None:intergenic	55.0%
	TTTGGGAGACGGTGCAGTTG+AGG	-	Chr6:25238810-25238829	MsG0680031716.01.T01:intron	55.0%
!	AGATCGGTTAATGGTGGGGC+GGG	+	Chr6:25238049-25238068	None:intergenic	55.0%
	ACACCAGACACACGATGCGC+CGG	+	Chr6:25237052-25237071	None:intergenic	60.0%
	GCAGGGATTCCTACCGGAAC+AGG	+	Chr6:25237683-25237702	None:intergenic	60.0%
	GTGGTTGAGCTTCTGGTCCG+AGG	-	Chr6:25240807-25240826	MsG0680031716.01.T01:intron	60.0%
	TGGTCGGCGCCTTTCGAACT+TGG	-	Chr6:25237655-25237674	MsG0680031716.01.T01:intron	60.0%
	CGCTGCGGTGCTTGGTACAG+TGG	+	Chr6:25240373-25240392	None:intergenic	65.0%
	TCTTACCGCGCTGCGGTGCT+TGG	+	Chr6:25240381-25240400	None:intergenic	65.0%
!	CTGTACCAAGCACCGCAGCG+CGG	-	Chr6:25240373-25240392	MsG0680031716.01.T01:intron	65.0%
	GCTCCGGCGCATCGTGTGTC+TGG	-	Chr6:25237046-25237065	MsG0680031716.01.T01:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	25236407	25241083	25236407	ID=MsG0680031716.01;Name=MsG0680031716.01
Chr6	mRNA	25236407	25241083	25236407	ID=MsG0680031716.01.T01;Parent=MsG0680031716.01;Name=MsG0680031716.01.T01;_AED=0.49;_eAED=0.60;_QI=270\|0.87\|0.77\|1\|0.62\|0.55\|9\|0\|297
Chr6	exon	25236407	25236687	25236407	ID=MsG0680031716.01.T01:exon:15532;Parent=MsG0680031716.01.T01
Chr6	exon	25237096	25237180	25237096	ID=MsG0680031716.01.T01:exon:15531;Parent=MsG0680031716.01.T01
Chr6	exon	25237343	25237415	25237343	ID=MsG0680031716.01.T01:exon:15530;Parent=MsG0680031716.01.T01
Chr6	exon	25237535	25237608	25237535	ID=MsG0680031716.01.T01:exon:15529;Parent=MsG0680031716.01.T01
Chr6	exon	25238032	25238106	25238032	ID=MsG0680031716.01.T01:exon:15528;Parent=MsG0680031716.01.T01
Chr6	exon	25238193	25238247	25238193	ID=MsG0680031716.01.T01:exon:15527;Parent=MsG0680031716.01.T01
Chr6	exon	25239174	25239247	25239174	ID=MsG0680031716.01.T01:exon:15526;Parent=MsG0680031716.01.T01
Chr6	exon	25239802	25240048	25239802	ID=MsG0680031716.01.T01:exon:15525;Parent=MsG0680031716.01.T01
Chr6	exon	25240884	25241083	25240884	ID=MsG0680031716.01.T01:exon:15524;Parent=MsG0680031716.01.T01
Chr6	five_prime_UTR	25240884	25241083	25240884	ID=MsG0680031716.01.T01:five_prime_utr;Parent=MsG0680031716.01.T01
Chr6	five_prime_UTR	25239979	25240048	25239979	ID=MsG0680031716.01.T01:five_prime_utr;Parent=MsG0680031716.01.T01
Chr6	CDS	25239802	25239978	25239802	ID=MsG0680031716.01.T01:cds;Parent=MsG0680031716.01.T01
Chr6	CDS	25239174	25239247	25239174	ID=MsG0680031716.01.T01:cds;Parent=MsG0680031716.01.T01
Chr6	CDS	25238193	25238247	25238193	ID=MsG0680031716.01.T01:cds;Parent=MsG0680031716.01.T01
Chr6	CDS	25238032	25238106	25238032	ID=MsG0680031716.01.T01:cds;Parent=MsG0680031716.01.T01
Chr6	CDS	25237535	25237608	25237535	ID=MsG0680031716.01.T01:cds;Parent=MsG0680031716.01.T01
Chr6	CDS	25237343	25237415	25237343	ID=MsG0680031716.01.T01:cds;Parent=MsG0680031716.01.T01
Chr6	CDS	25237096	25237180	25237096	ID=MsG0680031716.01.T01:cds;Parent=MsG0680031716.01.T01
Chr6	CDS	25236407	25236687	25236407	ID=MsG0680031716.01.T01:cds;Parent=MsG0680031716.01.T01

Gene Sequence

>MsG0680031716.01.T01

ATGATGAATGGAAGACAGACTCAAAATTGGGGTGAAAGTTCGAGTTCAACTTCAACTTCGCTTAGTAGTCAGCAGGATGTTGACGATGATCAGATGATTGCTCTTGTGCTATCGGAAGAGTATGCCAATTTAGATGGAGCAGTTGGTCGGCGCCTTTCGAACTTGGCACCTGTTCCGCATGTGCCCCGCATAAATACCTTCATCCCCACTATAAATGACGCCAGTATGGATCATCAGCGTCTGCTACAGAGGTTGGATGTTTATGGTCTTTGTGAAGTTAGAGTATCTGGAGATGGAAATTGTCAGTTCCGTGCACTTTCAGATCAGCTGTACCGGTCACCTGAACACCACAAGCATGTTCGTAAAGATATTGTAAAACAGCTTAAAGATCACCGTTCCTTATATGAATCCTATGTACCAATGAAGTACAAACGATATTACAAGAGAATGGCTAAATCTGGAGAATGGGGTGACCATGTTACCTTACAGGCAGCTTCTGATAAGGTTCGTAATGTTGTTTTGAATCAGTTTGCAGCTAAGATATGCCTTCTGACATCATTCAGAGATACTTGTTTCATCGAAATTATGCCACTGTACCAAGCACCGCAGCGCGAAGTGTGGTTGAGCTTCTGGTCCGAGGTTCATTACAACTCACTTTATGAAATTCGAGGTTTGTTCACCACTAAAACCGATCCTCTATTAAGCATAAGCATATTATTAGTAACTGATATATTATCTTGTGTTTATTTTAGCTGCACCAATCCAGCACAAACCAAGGAGGAAACATTGGTTGTTCTAGAAGATGTAGTGTCGTGTATGGCTCATCGAATGGTGCATATAATATCTAAAATGTTAGTATTCAGATATGAATACAAAGCATACATTATTCAGTGA

Protein sequence

>MsG0680031716.01.T01

MMNGRQTQNWGESSSSTSTSLSSQQDVDDDQMIALVLSEEYANLDGAVGRRLSNLAPVPHVPRINTFIPTINDASMDHQRLLQRLDVYGLCEVRVSGDGNCQFRALSDQLYRSPEHHKHVRKDIVKQLKDHRSLYESYVPMKYKRYYKRMAKSGEWGDHVTLQAASDKVRNVVLNQFAAKICLLTSFRDTCFIEIMPLYQAPQREVWLSFWSEVHYNSLYEIRGLFTTKTDPLLSISILLVTDILSCVYFSCTNPAQTKEETLVVLEDVVSCMAHRMVHIISKMLVFRYEYKAYIIQ*