Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042401.01.T01 | XP_013444170.1 | 64.286 | 280 | 43 | 3 | 3 | 281 | 1 | 224 | 1.91E-107 | 321 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042401.01.T01 | A0A072TL92 | 64.286 | 280 | 43 | 3 | 3 | 281 | 1 | 224 | 9.11e-108 | 321 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003673.01 | MsG0880042401.01 | 0.810581 | 1.073763e-50 | 6.497102e-48 |
| MsG0180003686.01 | MsG0880042401.01 | 0.817233 | 3.666403e-52 | 2.655079e-49 |
| MsG0180004389.01 | MsG0880042401.01 | 0.815379 | 9.527472e-52 | 6.558135e-49 |
| MsG0180004465.01 | MsG0880042401.01 | 0.819975 | 8.755918e-53 | 6.840020e-50 |
| MsG0880042401.01 | MsG0880045111.01 | 0.818674 | 1.732848e-52 | 1.305571e-49 |
| MsG0880042401.01 | MsG0880045736.01 | 0.819047 | 1.425762e-52 | 1.085388e-49 |
| MsG0380011609.01 | MsG0880042401.01 | 0.810949 | 8.936808e-51 | 5.461569e-48 |
| MsG0380012460.01 | MsG0880042401.01 | 0.800144 | 1.654407e-48 | 7.646043e-46 |
| MsG0380014537.01 | MsG0880042401.01 | 0.803709 | 3.062945e-49 | 1.549889e-46 |
| MsG0480018660.01 | MsG0880042401.01 | 0.801243 | 9.872563e-49 | 4.691771e-46 |
| MsG0480021465.01 | MsG0880042401.01 | 0.809084 | 2.253626e-50 | 1.310733e-47 |
| MsG0480022068.01 | MsG0880042401.01 | 0.805355 | 1.389466e-49 | 7.335456e-47 |
| MsG0480022799.01 | MsG0880042401.01 | 0.816147 | 6.423868e-52 | 4.515866e-49 |
| MsG0480022985.01 | MsG0880042401.01 | 0.802891 | 4.523695e-49 | 2.241686e-46 |
| MsG0580025758.01 | MsG0880042401.01 | 0.802369 | 5.796532e-49 | 2.834597e-46 |
| MsG0380015555.01 | MsG0880042401.01 | 0.813305 | 2.737279e-51 | 1.781102e-48 |
| MsG0380016064.01 | MsG0880042401.01 | 0.807989 | 3.859728e-50 | 2.180972e-47 |
| MsG0380017543.01 | MsG0880042401.01 | 0.813578 | 2.383865e-51 | 1.562732e-48 |
| MsG0480018236.01 | MsG0880042401.01 | 0.809410 | 1.918144e-50 | 1.125432e-47 |
| MsG0580029236.01 | MsG0880042401.01 | 0.802297 | 5.997481e-49 | 2.927485e-46 |
| MsG0680030413.01 | MsG0880042401.01 | 0.800164 | 1.638825e-48 | 7.577541e-46 |
| MsG0280007955.01 | MsG0880042401.01 | 0.817309 | 3.526320e-52 | 2.558869e-49 |
| MsG0280008112.01 | MsG0880042401.01 | 0.800083 | 1.702038e-48 | 7.854145e-46 |
| MsG0280008648.01 | MsG0880042401.01 | 0.806397 | 8.391544e-50 | 4.549788e-47 |
| MsG0780039170.01 | MsG0880042401.01 | 0.813999 | 1.926052e-51 | 1.277038e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042401.01.T01 | MTR_8g013040 | 64.286 | 280 | 43 | 3 | 3 | 281 | 1 | 224 | 2.31e-111 | 321 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042401.01.T01 | AT3G07150 | 35.662 | 272 | 91 | 8 | 13 | 281 | 9 | 199 | 2.25e-32 | 118 |
Find 37 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCCATAGCTAAAGCTTT+TGG | 0.234290 | 8:+8191369 | MsG0880042401.01.T01:CDS |
| GAAGGGTAAAACTAAGAAAT+AGG | 0.262972 | 8:-8192568 | None:intergenic |
| AAAGACATGTCAATGTTGTT+AGG | 0.283254 | 8:+8191246 | None:intergenic |
| CATGATGCTATTGTTGTTAC+TGG | 0.302910 | 8:+8191524 | MsG0880042401.01.T01:CDS |
| AAACAAACCAAATGTATAGA+AGG | 0.351386 | 8:-8192586 | None:intergenic |
| TTATTTCTTCTTCTGTTACT+TGG | 0.362087 | 8:+8191448 | MsG0880042401.01.T01:CDS |
| TCGTATAACTGCATGTAAAC+TGG | 0.378325 | 8:-8192688 | None:intergenic |
| GTGGTAGAAGGTGAGCATTC+TGG | 0.397667 | 8:+8192099 | MsG0880042401.01.T01:CDS |
| AAGTTGAGAGAGAGCAAATC+AGG | 0.414599 | 8:+8192171 | MsG0880042401.01.T01:CDS |
| CCATTCAACCTCAACGTTGA+TGG | 0.419649 | 8:-8191399 | None:intergenic |
| TCTTCTAGAAGTTTGTCTAC+TGG | 0.421279 | 8:+8191492 | MsG0880042401.01.T01:CDS |
| GGGATTGGCAAAGTGGTAGA+AGG | 0.433835 | 8:+8192087 | MsG0880042401.01.T01:CDS |
| CCATCAACGTTGAGGTTGAA+TGG | 0.438064 | 8:+8191399 | MsG0880042401.01.T01:CDS |
| TTAAAAGGTGAGATCTTGAA+TGG | 0.439116 | 8:+8192425 | MsG0880042401.01.T01:intron |
| AAGACATGTCAATGTTGTTA+GGG | 0.451521 | 8:+8191247 | None:intergenic |
| TTGTCGCAGCTAATTACAAA+AGG | 0.477652 | 8:+8192747 | MsG0880042401.01.T01:CDS |
| TTCAACATTAACAACTTCGA+TGG | 0.487046 | 8:+8192608 | MsG0880042401.01.T01:CDS |
| CATTCAACCTCAACGTTGAT+GGG | 0.496209 | 8:-8191398 | None:intergenic |
| ATGGTTACTTTATAAGTAGA+GGG | 0.496677 | 8:+8191418 | MsG0880042401.01.T01:CDS |
| AAAATAAACGAGGACAAGTC+AGG | 0.496765 | 8:+8192503 | MsG0880042401.01.T01:CDS |
| CTGGCTGCGAGAAATGGTTC+CGG | 0.501033 | 8:-8192669 | None:intergenic |
| AAACTAAGAAATAGGAGTGC+AGG | 0.507572 | 8:-8192560 | None:intergenic |
| TGTAAACTGGCTGCGAGAAA+TGG | 0.509703 | 8:-8192675 | None:intergenic |
| AATGGTTACTTTATAAGTAG+AGG | 0.510425 | 8:+8191417 | MsG0880042401.01.T01:CDS |
| TTAACAACTTCGATGGACTC+TGG | 0.516604 | 8:+8192615 | MsG0880042401.01.T01:CDS |
| TACTGGCAAAGTTGTCTACA+AGG | 0.521965 | 8:+8191541 | MsG0880042401.01.T01:CDS |
| GATAAAGTCGATGAGCTCTC+CGG | 0.526515 | 8:+8192650 | MsG0880042401.01.T01:CDS |
| TAACCCAAAAGCTTTAGCTA+TGG | 0.535559 | 8:-8191373 | None:intergenic |
| AACAAACCAAATGTATAGAA+GGG | 0.576831 | 8:-8192585 | None:intergenic |
| TCAAAATGGGATTGGCAAAG+TGG | 0.580663 | 8:+8192080 | MsG0880042401.01.T01:CDS |
| TGGTTACTTTATAAGTAGAG+GGG | 0.587269 | 8:+8191419 | MsG0880042401.01.T01:CDS |
| CAGTAACAACAATAGCATCA+TGG | 0.599261 | 8:-8191523 | None:intergenic |
| GGTTAGACCCATCAACGTTG+AGG | 0.608671 | 8:+8191391 | MsG0880042401.01.T01:CDS |
| GAGTGTACTTACTTATGCAG+AGG | 0.627304 | 8:+8192039 | MsG0880042401.01.T01:CDS |
| ATACGAGTAATAATGTGAAG+CGG | 0.657851 | 8:+8192705 | MsG0880042401.01.T01:CDS |
| ACTGGCAAAGTTGTCTACAA+GGG | 0.685525 | 8:+8191542 | MsG0880042401.01.T01:CDS |
| GAAGCGGATAAGAGAAGACG+AGG | 0.690208 | 8:+8192721 | MsG0880042401.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAAATCAATTTTACAGAA+TGG | + | Chr8:8191817-8191836 | MsG0880042401.01.T01:intron | 15.0% |
| !! | TACTTATTGAAAATGTTGAA+GGG | + | Chr8:8191639-8191658 | MsG0880042401.01.T01:intron | 20.0% |
| !! | TGTTCTTTGTTCTTATTAAA+AGG | + | Chr8:8192410-8192429 | MsG0880042401.01.T01:intron | 20.0% |
| !! | TTACTTATTGAAAATGTTGA+AGG | + | Chr8:8191638-8191657 | MsG0880042401.01.T01:intron | 20.0% |
| !!! | ATTCACAACATTTTCAAAAT+GGG | + | Chr8:8192067-8192086 | MsG0880042401.01.T01:CDS | 20.0% |
| !!! | ATTTTTCAGGAATATATAAC+TGG | + | Chr8:8192209-8192228 | MsG0880042401.01.T01:intron | 20.0% |
| !!! | GTCAAGTTTTATATTTTTGT+TGG | + | Chr8:8191572-8191591 | MsG0880042401.01.T01:CDS | 20.0% |
| !!! | TATGTCTTCATTTATTTTTC+AGG | + | Chr8:8192196-8192215 | MsG0880042401.01.T01:intron | 20.0% |
| ! | AAACAAACCAAATGTATAGA+AGG | - | Chr8:8192589-8192608 | None:intergenic | 25.0% |
| ! | AACAAACCAAATGTATAGAA+GGG | - | Chr8:8192588-8192607 | None:intergenic | 25.0% |
| ! | CAAGAAGTTAAAAATAAACG+AGG | + | Chr8:8192493-8192512 | MsG0880042401.01.T01:CDS | 25.0% |
| ! | TCAATTCTACTCATAACATT+AGG | - | Chr8:8191691-8191710 | None:intergenic | 25.0% |
| !! | AATGGTTACTTTATAAGTAG+AGG | + | Chr8:8191417-8191436 | MsG0880042401.01.T01:CDS | 25.0% |
| !! | ATGGTTACTTTATAAGTAGA+GGG | + | Chr8:8191418-8191437 | MsG0880042401.01.T01:CDS | 25.0% |
| !! | GATTCACAACATTTTCAAAA+TGG | + | Chr8:8192066-8192085 | MsG0880042401.01.T01:CDS | 25.0% |
| !! | TTATTTCTTCTTCTGTTACT+TGG | + | Chr8:8191448-8191467 | MsG0880042401.01.T01:CDS | 25.0% |
| !! | TTTTTGAATTTGAGAGTGAA+GGG | - | Chr8:8191308-8191327 | None:intergenic | 25.0% |
| ATACGAGTAATAATGTGAAG+CGG | + | Chr8:8192705-8192724 | MsG0880042401.01.T01:CDS | 30.0% | |
| GAAGGGTAAAACTAAGAAAT+AGG | - | Chr8:8192571-8192590 | None:intergenic | 30.0% | |
| GCAGGATATAGAAAAATAGA+GGG | - | Chr8:8192545-8192564 | None:intergenic | 30.0% | |
| TAAGTAAGTACACTCAATCT+AGG | - | Chr8:8192034-8192053 | None:intergenic | 30.0% | |
| TAGCAGACAATCATATACTT+TGG | - | Chr8:8192316-8192335 | None:intergenic | 30.0% | |
| TGCAGGATATAGAAAAATAG+AGG | - | Chr8:8192546-8192565 | None:intergenic | 30.0% | |
| TGTAATCATCTGTTGGAATT+CGG | - | Chr8:8192288-8192307 | None:intergenic | 30.0% | |
| TTAAAAGGTGAGATCTTGAA+TGG | + | Chr8:8192425-8192444 | MsG0880042401.01.T01:intron | 30.0% | |
| TTCAACATTAACAACTTCGA+TGG | + | Chr8:8192608-8192627 | MsG0880042401.01.T01:CDS | 30.0% | |
| ! | CAACATTTTCAAAATGGGAT+TGG | + | Chr8:8192072-8192091 | MsG0880042401.01.T01:CDS | 30.0% |
| ! | CTTTTTGAATTTGAGAGTGA+AGG | - | Chr8:8191309-8191328 | None:intergenic | 30.0% |
| ! | GATGATGAAAAAGTTGATCT+TGG | + | Chr8:8191348-8191367 | MsG0880042401.01.T01:CDS | 30.0% |
| ! | GTTTTACCCTTCTATACATT+TGG | + | Chr8:8192579-8192598 | MsG0880042401.01.T01:CDS | 30.0% |
| ! | TGGTTACTTTATAAGTAGAG+GGG | + | Chr8:8191419-8191438 | MsG0880042401.01.T01:CDS | 30.0% |
| AAAATAAACGAGGACAAGTC+AGG | + | Chr8:8192503-8192522 | MsG0880042401.01.T01:CDS | 35.0% | |
| AAGTACACTCAATCTAGGTA+AGG | - | Chr8:8192029-8192048 | None:intergenic | 35.0% | |
| AGACATCTAGAAATGACCAA+AGG | - | Chr8:8191895-8191914 | None:intergenic | 35.0% | |
| CAGTAACAACAATAGCATCA+TGG | - | Chr8:8191526-8191545 | None:intergenic | 35.0% | |
| GGTTACTTGTAATCATCTGT+TGG | - | Chr8:8192295-8192314 | None:intergenic | 35.0% | |
| TACGTTGCCACTATTCTAAA+AGG | - | Chr8:8192252-8192271 | None:intergenic | 35.0% | |
| TCGTATAACTGCATGTAAAC+TGG | - | Chr8:8192691-8192710 | None:intergenic | 35.0% | |
| TTGTCGCAGCTAATTACAAA+AGG | + | Chr8:8192747-8192766 | MsG0880042401.01.T01:CDS | 35.0% | |
| ! | AAACTAAGAAATAGGAGTGC+AGG | - | Chr8:8192563-8192582 | None:intergenic | 35.0% |
| ! | ACGTTGCCACTATTCTAAAA+GGG | - | Chr8:8192251-8192270 | None:intergenic | 35.0% |
| ! | CATGATGCTATTGTTGTTAC+TGG | + | Chr8:8191524-8191543 | MsG0880042401.01.T01:CDS | 35.0% |
| ! | GCGATTTTCAGTCAGTATTT+CGG | + | Chr8:8192347-8192366 | MsG0880042401.01.T01:intron | 35.0% |
| ! | GTTCCATAGCTAAAGCTTTT+GGG | + | Chr8:8191370-8191389 | MsG0880042401.01.T01:CDS | 35.0% |
| ! | GTTGAAAATTTTGTAGCAGC+TGG | + | Chr8:8191606-8191625 | MsG0880042401.01.T01:intron | 35.0% |
| ! | TAACCCAAAAGCTTTAGCTA+TGG | - | Chr8:8191376-8191395 | None:intergenic | 35.0% |
| ! | TCTTCTAGAAGTTTGTCTAC+TGG | + | Chr8:8191492-8191511 | MsG0880042401.01.T01:CDS | 35.0% |
| !!! | AATGTCCCCTTTTAGAATAG+TGG | + | Chr8:8192242-8192261 | MsG0880042401.01.T01:intron | 35.0% |
| AAGTTGAGAGAGAGCAAATC+AGG | + | Chr8:8192171-8192190 | MsG0880042401.01.T01:CDS | 40.0% | |
| ACTGGCAAAGTTGTCTACAA+GGG | + | Chr8:8191542-8191561 | MsG0880042401.01.T01:CDS | 40.0% | |
| AGGGTGTGTTTGTGTATAGA+GGG | + | Chr8:8191658-8191677 | MsG0880042401.01.T01:intron | 40.0% | |
| ATAATTCACGATCACCACCA+AGG | + | Chr8:8191992-8192011 | MsG0880042401.01.T01:intron | 40.0% | |
| CATTCAACCTCAACGTTGAT+GGG | - | Chr8:8191401-8191420 | None:intergenic | 40.0% | |
| GAGTGTACTTACTTATGCAG+AGG | + | Chr8:8192039-8192058 | MsG0880042401.01.T01:CDS | 40.0% | |
| GTAAGGAAGTTCTATCACCT+TGG | - | Chr8:8192012-8192031 | None:intergenic | 40.0% | |
| GTGTTTGTGTATAGAGGGTA+GGG | + | Chr8:8191663-8191682 | MsG0880042401.01.T01:intron | 40.0% | |
| TACTGGCAAAGTTGTCTACA+AGG | + | Chr8:8191541-8191560 | MsG0880042401.01.T01:CDS | 40.0% | |
| TGTGTTTGTGTATAGAGGGT+AGG | + | Chr8:8191662-8191681 | MsG0880042401.01.T01:intron | 40.0% | |
| ! | AAGGGTGTGTTTGTGTATAG+AGG | + | Chr8:8191657-8191676 | MsG0880042401.01.T01:intron | 40.0% |
| ! | CGTTGCCACTATTCTAAAAG+GGG | - | Chr8:8192250-8192269 | None:intergenic | 40.0% |
| ! | GGTTCCATAGCTAAAGCTTT+TGG | + | Chr8:8191369-8191388 | MsG0880042401.01.T01:CDS | 40.0% |
| ! | TCAAAATGGGATTGGCAAAG+TGG | + | Chr8:8192080-8192099 | MsG0880042401.01.T01:CDS | 40.0% |
| ! | TTAACAACTTCGATGGACTC+TGG | + | Chr8:8192615-8192634 | MsG0880042401.01.T01:CDS | 40.0% |
| AGGAAGTTCTATCACCTTGG+TGG | - | Chr8:8192009-8192028 | None:intergenic | 45.0% | |
| CAGTATTTCGGTACGCTTCT+AGG | + | Chr8:8192359-8192378 | MsG0880042401.01.T01:intron | 45.0% | |
| CCATCAACGTTGAGGTTGAA+TGG | + | Chr8:8191399-8191418 | MsG0880042401.01.T01:CDS | 45.0% | |
| CCATTCAACCTCAACGTTGA+TGG | - | Chr8:8191402-8191421 | None:intergenic | 45.0% | |
| GATAAAGTCGATGAGCTCTC+CGG | + | Chr8:8192650-8192669 | MsG0880042401.01.T01:CDS | 45.0% | |
| GTGTGCTTAGAGTCTCACAT+TGG | + | Chr8:8191952-8191971 | MsG0880042401.01.T01:intron | 45.0% | |
| TGTAAACTGGCTGCGAGAAA+TGG | - | Chr8:8192678-8192697 | None:intergenic | 45.0% | |
| !!! | AACCGTGTGTGTTGGTTTTG+CGG | - | Chr8:8191867-8191886 | None:intergenic | 45.0% |
| AGGAAGCTAACCGTGTGTGT+TGG | - | Chr8:8191875-8191894 | None:intergenic | 50.0% | |
| CACCGCAAAACCAACACACA+CGG | + | Chr8:8191862-8191881 | MsG0880042401.01.T01:intron | 50.0% | |
| CATTGGATGCTGACGTGGAA+CGG | + | Chr8:8191969-8191988 | MsG0880042401.01.T01:intron | 50.0% | |
| GAAGCGGATAAGAGAAGACG+AGG | + | Chr8:8192721-8192740 | MsG0880042401.01.T01:CDS | 50.0% | |
| GGTTAGACCCATCAACGTTG+AGG | + | Chr8:8191391-8191410 | MsG0880042401.01.T01:CDS | 50.0% | |
| GTGGTAGAAGGTGAGCATTC+TGG | + | Chr8:8192099-8192118 | MsG0880042401.01.T01:CDS | 50.0% | |
| TCTCACATTGGATGCTGACG+TGG | + | Chr8:8191964-8191983 | MsG0880042401.01.T01:intron | 50.0% | |
| ! | GGGATTGGCAAAGTGGTAGA+AGG | + | Chr8:8192087-8192106 | MsG0880042401.01.T01:CDS | 50.0% |
| !! | CACACACGGTTAGCTTCCTT+TGG | + | Chr8:8191876-8191895 | MsG0880042401.01.T01:intron | 50.0% |
| CTGGCTGCGAGAAATGGTTC+CGG | - | Chr8:8192672-8192691 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 8191252 | 8192778 | 8191252 | ID=MsG0880042401.01;Name=MsG0880042401.01 |
| Chr8 | mRNA | 8191252 | 8192778 | 8191252 | ID=MsG0880042401.01.T01;Parent=MsG0880042401.01;Name=MsG0880042401.01.T01;_AED=0.20;_eAED=0.28;_QI=0|0|0|1|0.5|0.33|3|0|281 |
| Chr8 | exon | 8191252 | 8191593 | 8191252 | ID=MsG0880042401.01.T01:exon:3111;Parent=MsG0880042401.01.T01 |
| Chr8 | exon | 8192036 | 8192192 | 8192036 | ID=MsG0880042401.01.T01:exon:3110;Parent=MsG0880042401.01.T01 |
| Chr8 | exon | 8192432 | 8192778 | 8192432 | ID=MsG0880042401.01.T01:exon:3109;Parent=MsG0880042401.01.T01 |
| Chr8 | CDS | 8191252 | 8191593 | 8191252 | ID=MsG0880042401.01.T01:cds;Parent=MsG0880042401.01.T01 |
| Chr8 | CDS | 8192036 | 8192192 | 8192036 | ID=MsG0880042401.01.T01:cds;Parent=MsG0880042401.01.T01 |
| Chr8 | CDS | 8192432 | 8192778 | 8192432 | ID=MsG0880042401.01.T01:cds;Parent=MsG0880042401.01.T01 |
| Gene Sequence |
| Protein sequence |
