AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007069.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007069.01.T01 MTR_2g019900 100.000 102 0 0 1 102 1 102 4.19e-72 209
MsG0280007069.01.T01 MTR_4g119050 78.641 103 21 1 1 102 1 103 5.36e-56 169
MsG0280007069.01.T01 MTR_4g119030 78.641 103 21 1 1 102 1 103 5.36e-56 169
MsG0280007069.01.T01 MTR_4g119040 78.641 103 21 1 1 102 1 103 5.36e-56 169
MsG0280007069.01.T01 MTR_2g019950 63.725 102 37 0 1 102 1 102 1.05e-44 140
MsG0280007069.01.T01 MTR_1g088905 53.922 102 47 0 1 102 1 102 8.57e-40 128
MsG0280007069.01.T01 MTR_7g108250 52.941 102 48 0 1 102 1 102 8.76e-40 127
MsG0280007069.01.T01 MTR_1g088910 58.416 101 42 0 1 101 1 101 4.64e-39 126
MsG0280007069.01.T01 MTR_1g088920 58.947 95 39 0 1 95 1 95 6.29e-39 125
MsG0280007069.01.T01 MTR_1g088925 56.842 95 41 0 1 95 1 95 8.01e-39 125
MsG0280007069.01.T01 MTR_7g108200 55.789 95 42 0 1 95 1 95 2.79e-37 121
MsG0280007069.01.T01 MTR_7g022710 53.398 103 47 1 1 102 30 132 9.41e-37 121
MsG0280007069.01.T01 MTR_7g026770 57.843 102 42 1 1 101 22 123 2.02e-36 125
MsG0280007069.01.T01 MTR_7g108220 55.882 102 44 1 1 101 1 102 2.71e-36 119
MsG0280007069.01.T01 MTR_7g022690 52.427 103 48 1 1 102 30 132 7.59e-36 119
MsG0280007069.01.T01 MTR_7g108210 54.902 102 45 1 1 101 1 102 1.80e-35 117
MsG0280007069.01.T01 MTR_7g022550 51.456 103 49 1 1 102 30 132 3.63e-35 117
MsG0280007069.01.T01 MTR_1g088895 50.000 102 51 0 1 102 1 102 5.49e-31 105
MsG0280007069.01.T01 MTR_7g108260 44.554 101 56 0 1 101 1 101 8.68e-29 100
MsG0280007069.01.T01 MTR_2g048970 46.667 105 52 1 2 102 29 133 3.26e-25 92.0
MsG0280007069.01.T01 MTR_2g014760 41.346 104 51 3 4 102 29 127 4.33e-23 86.7
MsG0280007069.01.T01 MTR_2g090755 37.864 103 57 2 4 102 41 140 1.30e-22 85.5
MsG0280007069.01.T01 MTR_1g015890 44.118 102 55 1 3 102 48 149 2.42e-21 82.8
MsG0280007069.01.T01 MTR_4g079110 39.000 100 56 2 6 102 58 155 3.36e-21 82.4
MsG0280007069.01.T01 MTR_7g035245 40.000 100 60 0 1 100 3 102 1.46e-20 79.3
MsG0280007069.01.T01 MTR_3g077570 38.000 100 62 0 1 100 18 117 3.07e-19 76.6
MsG0280007069.01.T01 MTR_3g077560 37.000 100 63 0 1 100 21 120 2.54e-18 74.3
MsG0280007069.01.T01 MTR_3g104510 39.604 101 53 3 3 101 23 117 1.36e-17 72.4
MsG0280007069.01.T01 MTR_5g077550 40.777 103 59 1 2 102 21 123 2.54e-16 68.9
MsG0280007069.01.T01 MTR_1g069255 36.364 99 62 1 2 99 84 182 4.99e-15 67.4
MsG0280007069.01.T01 MTR_2g038560 32.258 93 63 0 2 94 29 121 1.32e-11 57.0
MsG0280007069.01.T01 MTR_2g038560 33.333 84 56 0 2 85 70 153 2.57e-11 57.4
MsG0280007069.01.T01 MTR_2g038560 33.333 84 56 0 2 85 73 156 2.61e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007069.01.T01 AT5G18600 76.471 102 24 0 1 102 1 102 2.92e-55 167
MsG0280007069.01.T01 AT4G15690 72.549 102 28 0 1 102 1 102 6.57e-55 166
MsG0280007069.01.T01 AT4G15680 69.608 102 31 0 1 102 1 102 4.95e-54 164
MsG0280007069.01.T01 AT4G15670 69.608 102 31 0 1 102 1 102 1.42e-53 162
MsG0280007069.01.T01 AT4G15700 70.588 102 30 0 1 102 1 102 2.43e-53 162
MsG0280007069.01.T01 AT4G15660 68.627 102 32 0 1 102 1 102 4.80e-52 159
MsG0280007069.01.T01 AT1G03020 60.784 102 40 0 1 102 1 102 9.73e-44 138
MsG0280007069.01.T01 AT3G62930 59.804 102 41 0 1 102 1 102 1.13e-42 135
MsG0280007069.01.T01 AT3G21460 59.804 102 41 0 1 102 1 102 2.24e-41 132
MsG0280007069.01.T01 AT2G47870 57.282 103 43 1 1 102 1 103 4.02e-40 129
MsG0280007069.01.T01 AT1G06830 55.789 95 42 0 1 95 1 95 8.86e-39 125
MsG0280007069.01.T01 AT2G47880 54.000 100 46 0 1 100 1 100 2.30e-38 124
MsG0280007069.01.T01 AT3G62950 53.398 103 47 1 1 102 1 103 2.82e-38 124
MsG0280007069.01.T01 AT2G30540 55.789 95 42 0 1 95 1 95 5.47e-38 123
MsG0280007069.01.T01 AT3G62960 52.632 95 45 0 1 95 1 95 1.16e-35 117
MsG0280007069.01.T01 AT5G14070 49.091 110 48 2 1 102 31 140 9.35e-33 111
MsG0280007069.01.T01 AT3G02000 52.830 106 44 1 3 102 31 136 2.20e-31 107
MsG0280007069.01.T01 AT4G33040 42.478 113 43 3 3 102 41 144 3.38e-21 82.0
MsG0280007069.01.T01 AT1G28480 35.238 105 64 1 2 102 33 137 1.07e-20 80.9
MsG0280007069.01.T01 AT5G63030 39.796 98 59 0 3 100 21 118 1.02e-19 77.8
MsG0280007069.01.T01 AT5G11930 44.000 100 46 3 3 101 57 147 1.87e-19 77.8
MsG0280007069.01.T01 AT5G40370 37.000 100 63 0 1 100 3 102 9.19e-19 75.1
MsG0280007069.01.T01 AT1G03850 37.634 93 54 1 10 102 62 150 2.34e-16 70.1
MsG0280007069.01.T01 AT4G28730 37.113 97 60 1 2 97 71 167 1.82e-14 65.5
MsG0280007069.01.T01 AT5G40370 38.372 86 53 0 15 100 42 127 1.46e-13 62.4
MsG0280007069.01.T01 AT2G20270 35.052 97 62 1 2 97 76 172 2.42e-13 62.8
MsG0280007069.01.T01 AT5G20500 34.737 95 62 0 4 98 35 129 2.07e-12 59.3

Find 26 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGAAGAGCAGGAGCTATT+TGG 0.243476 2:-10143480 MsG0280007069.01.T01:CDS
TCATCAAGTTCATGAACTTC+TGG 0.278380 2:+10143649 None:intergenic
TTGGAATCAAAGACCTATTA+AGG 0.291286 2:+10143506 None:intergenic
TTATGAGCCTTCACCTTAAT+AGG 0.313253 2:-10143519 MsG0280007069.01.T01:CDS
TAGAAGAGCAGGAGCTATTT+GGG 0.338414 2:-10143479 MsG0280007069.01.T01:CDS
TCTGTGCCTGCTGTGTTCAT+TGG 0.345825 2:-10143571 MsG0280007069.01.T01:CDS
AAAACCCTCTTCAGTGACTT+TGG 0.383168 2:-10143679 MsG0280007069.01.T01:CDS
TTCATTGGTGGTGAGCTTGT+TGG 0.448261 2:-10143556 MsG0280007069.01.T01:CDS
GTGCCTGCTGTGTTCATTGG+TGG 0.469372 2:-10143568 MsG0280007069.01.T01:CDS
ATTCCAATGCTTAGAAGAGC+AGG 0.473528 2:-10143490 MsG0280007069.01.T01:CDS
GCTCCTGCTCTTCTAAGCAT+TGG 0.494845 2:+10143487 None:intergenic
ATTGGTGGTGAGCTTGTTGG+TGG 0.505118 2:-10143553 MsG0280007069.01.T01:CDS
CACTCCAAAGTCACTGAAGA+GGG 0.526613 2:+10143675 None:intergenic
ATGAACACAGCAGGCACAGA+AGG 0.552108 2:+10143574 None:intergenic
TCACCACCAATGAACACAGC+AGG 0.561831 2:+10143565 None:intergenic
TCAAAGACCTATTAAGGTGA+AGG 0.569141 2:+10143512 None:intergenic
CAACCTTACATTAATGGAGA+GGG 0.591764 2:-10143773 None:intergenic
AATGGAGAGGGTGACACAGT+TGG 0.593802 2:-10143761 MsG0280007069.01.T01:CDS
AACTTGATGAAATGAGTGGA+GGG 0.608563 2:-10143636 MsG0280007069.01.T01:CDS
TCAACCTTACATTAATGGAG+AGG 0.626935 2:-10143774 None:intergenic
TCACTCCAAAGTCACTGAAG+AGG 0.628179 2:+10143674 None:intergenic
TGAACACAGCAGGCACAGAA+GGG 0.649613 2:+10143575 None:intergenic
CATGAACTTGATGAAATGAG+TGG 0.667717 2:-10143640 MsG0280007069.01.T01:CDS
GAACTTGATGAAATGAGTGG+AGG 0.668923 2:-10143637 MsG0280007069.01.T01:CDS
GTTGGCATCAGAGAGGTCAG+TGG 0.695839 2:-10143743 MsG0280007069.01.T01:CDS
TGACACAGTTGGCATCAGAG+AGG 0.724593 2:-10143750 MsG0280007069.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGGAATCAAAGACCTATTA+AGG + Chr2:10143731-10143750 None:intergenic 30.0%
! ACATTGAACAAGCACTTTTA+AGG - Chr2:10143625-10143644 MsG0280007069.01.T01:CDS 30.0%
AACTTGATGAAATGAGTGGA+GGG - Chr2:10143598-10143617 MsG0280007069.01.T01:CDS 35.0%
CATGAACTTGATGAAATGAG+TGG - Chr2:10143594-10143613 MsG0280007069.01.T01:CDS 35.0%
TCAAAGACCTATTAAGGTGA+AGG + Chr2:10143725-10143744 None:intergenic 35.0%
TCATCAAGTTCATGAACTTC+TGG + Chr2:10143588-10143607 None:intergenic 35.0%
TTATGAGCCTTCACCTTAAT+AGG - Chr2:10143715-10143734 MsG0280007069.01.T01:CDS 35.0%
AAAACCCTCTTCAGTGACTT+TGG - Chr2:10143555-10143574 MsG0280007069.01.T01:CDS 40.0%
ATTCCAATGCTTAGAAGAGC+AGG - Chr2:10143744-10143763 MsG0280007069.01.T01:CDS 40.0%
GAACTTGATGAAATGAGTGG+AGG - Chr2:10143597-10143616 MsG0280007069.01.T01:CDS 40.0%
TAGAAGAGCAGGAGCTATTT+GGG - Chr2:10143755-10143774 MsG0280007069.01.T01:CDS 40.0%
TTAGAAGAGCAGGAGCTATT+TGG - Chr2:10143754-10143773 MsG0280007069.01.T01:CDS 40.0%
!!! GAACAAGCACTTTTAAGGCT+AGG - Chr2:10143630-10143649 MsG0280007069.01.T01:CDS 40.0%
CACTCCAAAGTCACTGAAGA+GGG + Chr2:10143562-10143581 None:intergenic 45.0%
TCACTCCAAAGTCACTGAAG+AGG + Chr2:10143563-10143582 None:intergenic 45.0%
!! TTCATTGGTGGTGAGCTTGT+TGG - Chr2:10143678-10143697 MsG0280007069.01.T01:CDS 45.0%
ATGAACACAGCAGGCACAGA+AGG + Chr2:10143663-10143682 None:intergenic 50.0%
GCTCCTGCTCTTCTAAGCAT+TGG + Chr2:10143750-10143769 None:intergenic 50.0%
TCACCACCAATGAACACAGC+AGG + Chr2:10143672-10143691 None:intergenic 50.0%
TCTGTGCCTGCTGTGTTCAT+TGG - Chr2:10143663-10143682 MsG0280007069.01.T01:CDS 50.0%
TGAACACAGCAGGCACAGAA+GGG + Chr2:10143662-10143681 None:intergenic 50.0%
TGACACAGTTGGCATCAGAG+AGG - Chr2:10143484-10143503 MsG0280007069.01.T01:CDS 50.0%
!! ATTGGTGGTGAGCTTGTTGG+TGG - Chr2:10143681-10143700 MsG0280007069.01.T01:CDS 50.0%
GTGCCTGCTGTGTTCATTGG+TGG - Chr2:10143666-10143685 MsG0280007069.01.T01:CDS 55.0%
GTTGGCATCAGAGAGGTCAG+TGG - Chr2:10143491-10143510 MsG0280007069.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 10143474 10143782 10143474 ID=MsG0280007069.01;Name=MsG0280007069.01
Chr2 mRNA 10143474 10143782 10143474 ID=MsG0280007069.01.T01;Parent=MsG0280007069.01;Name=MsG0280007069.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|102
Chr2 exon 10143474 10143782 10143474 ID=MsG0280007069.01.T01:exon:3164;Parent=MsG0280007069.01.T01
Chr2 CDS 10143474 10143782 10143474 ID=MsG0280007069.01.T01:cds;Parent=MsG0280007069.01.T01
Gene Sequence

>MsG0280007069.01.T01

ATGGAGAGGGTGACACAGTTGGCATCAGAGAGGTCAGTGGTGATATTCAGCAAGAGTAGTTGTTGCATGTGTCACACAATCAAAACCCTCTTCAGTGACTTTGGAGTGAATCCAGAAGTTCATGAACTTGATGAAATGAGTGGAGGGAGAGACATTGAACAAGCACTTTTAAGGCTAGGATGTAACCCTTCTGTGCCTGCTGTGTTCATTGGTGGTGAGCTTGTTGGTGGAGCTAATGAAGTTATGAGCCTTCACCTTAATAGGTCTTTGATTCCAATGCTTAGAAGAGCAGGAGCTATTTGGGTTTGA

Protein sequence

>MsG0280007069.01.T01

MERVTQLASERSVVIFSKSSCCMCHTIKTLFSDFGVNPEVHELDEMSGGRDIEQALLRLGCNPSVPAVFIGGELVGGANEVMSLHLNRSLIPMLRRAGAIWV*