AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0010850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 59 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAAGCATCATATTTAAT+TGG 0.234173 1_1:-16397515 None:intergenic
CAAAGGGCCACCAGCCAAAA+TGG 0.260033 1_1:-16397639 None:intergenic
GAAATGCACCTGTGTATTAT+AGG 0.318046 1_1:-16396966 None:intergenic
CTTTCATCCTCCTCAATGTT+GGG 0.343582 1_1:-16397485 None:intergenic
TATATTCCTCGCATCATGAT+TGG 0.373746 1_1:+16397725 Msa0010850:CDS
TACTTTCTTCCGCGAGTTGA+GGG 0.377617 1_1:+16397796 Msa0010850:CDS
AGACATGGACGACATGCATC+TGG 0.383730 1_1:+16395307 Msa0010850:five_prime_UTR
ATAAATTAGGCAAAAGATCT+TGG 0.388795 1_1:+16396864 Msa0010850:intron
TCCGTACCATGTATATAATA+TGG 0.393208 1_1:-16396923 None:intergenic
TGACCGGGTGTTGAAGAATA+TGG 0.396892 1_1:+16397443 Msa0010850:five_prime_UTR
ATTTCCAATAACCAAATAAA+CGG 0.397782 1_1:-16397854 None:intergenic
TAGAATGAGAACAGGGGTTA+TGG 0.398965 1_1:-16395385 None:intergenic
ACCCCTGTTCTCATTCTAAC+TGG 0.400791 1_1:+16395390 Msa0010850:five_prime_UTR
TTAGGTGTGCCGCATATATA+CGG 0.414944 1_1:+16397764 Msa0010850:CDS
ATTAATGGAGCAAGAGTTGC+GGG 0.416432 1_1:+16397331 Msa0010850:five_prime_UTR
CTCGCGGAAGAAAGTACAAA+AGG 0.434639 1_1:-16397789 None:intergenic
AAGCGTTGATCGACGGTTTG+AGG 0.444795 1_1:+16397397 Msa0010850:five_prime_UTR
ATATTCCTCGCATCATGATT+GGG 0.449764 1_1:+16397726 Msa0010850:CDS
CGCAAGCACATCAAGGTGAC+CGG 0.462789 1_1:+16397427 Msa0010850:five_prime_UTR
GATGGTTGTTTGCTAAGGAC+TGG 0.465574 1_1:-16395357 None:intergenic
ACTTTCATCCTCCTCAATGT+TGG 0.469316 1_1:-16397486 None:intergenic
GTACTTTCTTCCGCGAGTTG+AGG 0.471488 1_1:+16397795 Msa0010850:CDS
TCACCAGTTAGAATGAGAAC+AGG 0.473651 1_1:-16395393 None:intergenic
ATGCAGGGATATGCTATTGT+CGG 0.496724 1_1:+16397674 Msa0010850:CDS
TCGATGAAGCTCGTCTACAT+AGG 0.503727 1_1:-16396895 None:intergenic
GCATCAAAATTGAACACAAA+GGG 0.507153 1_1:-16397655 None:intergenic
TTCAAGAAAGCGTTGATCGA+CGG 0.516090 1_1:+16397390 Msa0010850:five_prime_UTR
ACCATATTATATACATGGTA+CGG 0.523844 1_1:+16396922 Msa0010850:five_prime_UTR
CGATGAAGCTCGTCTACATA+GGG 0.534160 1_1:-16396894 None:intergenic
GTTGCGGGACAAGATGGAAC+GGG 0.537406 1_1:+16397346 Msa0010850:five_prime_UTR
GCAAGAGTTGCGGGACAAGA+TGG 0.542408 1_1:+16397340 Msa0010850:five_prime_UTR
ATGGTTGTTTGCTAAGGACT+GGG 0.556392 1_1:-16395356 None:intergenic
AGAATATGGTTCAGATGAGT+CGG 0.556570 1_1:+16397457 Msa0010850:five_prime_UTR
AGTCATACACACACAAGAAG+AGG 0.560352 1_1:+16395252 Msa0010850:five_prime_UTR
ACAGGGGTTATGGTGAGAGA+TGG 0.575227 1_1:-16395375 None:intergenic
AAGAAGAGGATGCGGTGAAG+AGG 0.575707 1_1:+16395266 Msa0010850:five_prime_UTR
TGCATCAAAATTGAACACAA+AGG 0.580420 1_1:-16397656 None:intergenic
CACCAGTTAGAATGAGAACA+GGG 0.593749 1_1:-16395392 None:intergenic
TGAGAGATGGTTGTTTGCTA+AGG 0.599143 1_1:-16395362 None:intergenic
AATTGATCGCAAGCACATCA+AGG 0.602229 1_1:+16397420 Msa0010850:five_prime_UTR
AAATATATTCATCGACGAGA+GGG 0.603737 1_1:-16397830 None:intergenic
GACATGGACGACATGCATCT+GGG 0.604335 1_1:+16395308 Msa0010850:five_prime_UTR
TCGCGGAAGAAAGTACAAAA+GGG 0.604568 1_1:-16397788 None:intergenic
GCGAGAAAAGAAAGAAGACA+TGG 0.608113 1_1:+16395292 Msa0010850:five_prime_UTR
GCTAAGGACTGGGTATTGAA+CGG 0.608410 1_1:-16395346 None:intergenic
TATTAATGGAGCAAGAGTTG+CGG 0.610755 1_1:+16397330 Msa0010850:five_prime_UTR
AGAAAACCATATTATATACA+TGG 0.615855 1_1:+16396917 Msa0010850:five_prime_UTR
AGTTGCGGGACAAGATGGAA+CGG 0.623306 1_1:+16397345 Msa0010850:five_prime_UTR
GTCGGACAATCCCAACATTG+AGG 0.630524 1_1:+16397475 Msa0010850:five_prime_UTR
GCAAGCACATCAAGGTGACC+GGG 0.633386 1_1:+16397428 Msa0010850:five_prime_UTR
AAAATATATTCATCGACGAG+AGG 0.634794 1_1:-16397831 None:intergenic
GAACCATATTCTTCAACACC+CGG 0.652425 1_1:-16397446 None:intergenic
TAAGACACACCCTCAACTCG+CGG 0.652976 1_1:-16397805 None:intergenic
CAAGACCCAATCATGATGCG+AGG 0.663868 1_1:-16397731 None:intergenic
ACACACACAAGAAGAGGATG+CGG 0.666749 1_1:+16395258 Msa0010850:five_prime_UTR
TGATCGCTCCTATAATACAC+AGG 0.675215 1_1:+16396958 Msa0010850:five_prime_UTR
GGACAATCCCAACATTGAGG+AGG 0.682139 1_1:+16397478 Msa0010850:five_prime_UTR
CAAAAGGGACCGTATATATG+CGG 0.692290 1_1:-16397773 None:intergenic
ACCAGTTAGAATGAGAACAG+GGG 0.770116 1_1:-16395391 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTGTTTTTTGACTTA+AGG + chr1_1:16395604-16395623 Msa0010850:intron 15.0%
!!! TATATTTAACAATTTTCCTT+TGG + chr1_1:16395855-16395874 Msa0010850:intron 15.0%
!! TAATTAAATACTAAGATTAG+TGG + chr1_1:16396517-16396536 Msa0010850:intron 15.0%
!!! TTAACTTTTTGTGATAAATT+AGG + chr1_1:16396851-16396870 Msa0010850:intron 15.0%
!!! AGGTTTTTTTTTTCAGATTT+AGG + chr1_1:16395429-16395448 Msa0010850:intron 20.0%
!!! GGTTTTTTTTTTCAGATTTA+GGG + chr1_1:16395430-16395449 Msa0010850:intron 20.0%
!!! TTTTTTTTTCTTTCAGATCT+AGG + chr1_1:16395491-16395510 Msa0010850:intron 20.0%
!!! TTTTTTTTCTTTCAGATCTA+GGG + chr1_1:16395492-16395511 Msa0010850:intron 20.0%
!!! TTAAGGATTTCTATGTTTTA+GGG + chr1_1:16395621-16395640 Msa0010850:intron 20.0%
!!! TAGCAGTATTAGTTTTAAAA+GGG - chr1_1:16395757-16395776 None:intergenic 20.0%
!!! TTAAGTTATCAGTCAATTTT+TGG + chr1_1:16396210-16396229 Msa0010850:intron 20.0%
!! AAGTATTATAGGTTCTATAA+AGG - chr1_1:16396637-16396656 None:intergenic 20.0%
!! AATGGTATATAATTGCATAA+TGG - chr1_1:16396697-16396716 None:intergenic 20.0%
!! ACGTATATATTTAATTAGTC+AGG + chr1_1:16396816-16396835 Msa0010850:intron 20.0%
!! AGAAAACCATATTATATACA+TGG + chr1_1:16396917-16396936 Msa0010850:five_prime_UTR 20.0%
!!! GAATATATTTTCCGTTTATT+TGG + chr1_1:16397843-16397862 Msa0010850:exon 20.0%
!!! TTTCTATGTTTATGGTTTTG+TGG + chr1_1:16395516-16395535 Msa0010850:intron 25.0%
! TTAGATTTAGGGATTTCTTA+TGG + chr1_1:16395569-16395588 Msa0010850:intron 25.0%
!! CTTAAGGATTTCTATGTTTT+AGG + chr1_1:16395620-16395639 Msa0010850:intron 25.0%
!!! GTAGCAGTATTAGTTTTAAA+AGG - chr1_1:16395758-16395777 None:intergenic 25.0%
! ATGACAAAAAAACATATGCA+GGG + chr1_1:16395779-16395798 Msa0010850:intron 25.0%
! TTTGTGATTTGAATTTCCAA+AGG - chr1_1:16395874-16395893 None:intergenic 25.0%
! ATTCAAATCACAAAGACAAT+TGG + chr1_1:16395880-16395899 Msa0010850:intron 25.0%
! TTCAAATCACAAAGACAATT+GGG + chr1_1:16395881-16395900 Msa0010850:intron 25.0%
! TTGATATATAAAGCGAAACT+AGG + chr1_1:16396119-16396138 Msa0010850:intron 25.0%
! TGATATATAAAGCGAAACTA+GGG + chr1_1:16396120-16396139 Msa0010850:intron 25.0%
!!! TTTGTTTTTGTTGTGTTATG+CGG + chr1_1:16396441-16396460 Msa0010850:intron 25.0%
! TACGTGTAAAAACATGTTAT+AGG - chr1_1:16396547-16396566 None:intergenic 25.0%
! TATTGCTAAACTTTGTGAAA+AGG + chr1_1:16396565-16396584 Msa0010850:intron 25.0%
! CTCAAGTAATCAAGTATTAT+AGG - chr1_1:16396648-16396667 None:intergenic 25.0%
! TTTGAAAAGTTATAGCACAA+TGG - chr1_1:16396715-16396734 None:intergenic 25.0%
! AAACCTTCACAATAAACATA+TGG + chr1_1:16396757-16396776 Msa0010850:intron 25.0%
! TTACCATATGTTTATTGTGA+AGG - chr1_1:16396763-16396782 None:intergenic 25.0%
! ATAAATTAGGCAAAAGATCT+TGG + chr1_1:16396864-16396883 Msa0010850:intron 25.0%
! ACCATATTATATACATGGTA+CGG + chr1_1:16396922-16396941 Msa0010850:five_prime_UTR 25.0%
! AATAGTAACATGAATATTCC+TGG - chr1_1:16397048-16397067 None:intergenic 25.0%
!! TTTTGTGCATGTGATATTAA+TGG + chr1_1:16397316-16397335 Msa0010850:five_prime_UTR 25.0%
!! TCTGAAGCATCATATTTAAT+TGG - chr1_1:16397518-16397537 None:intergenic 25.0%
!!! ATGATGCTTTAGATAGTTTT+AGG + chr1_1:16397570-16397589 Msa0010850:intron 25.0%
!!! GATAGTTTTAGGATAGTTTT+AGG + chr1_1:16397581-16397600 Msa0010850:intron 25.0%
!!! GGATAGTTTTAGATAGTTTT+AGG + chr1_1:16397602-16397621 Msa0010850:intron 25.0%
!!! ATAGTTTTAGGCTAGTTTTT+AGG + chr1_1:16397614-16397633 Msa0010850:five_prime_UTR 25.0%
TTTAGGGATTTCGATGTTTA+TGG + chr1_1:16395446-16395465 Msa0010850:intron 30.0%
!! TTTCGATGTTTATGGTTTTG+TGG + chr1_1:16395454-16395473 Msa0010850:intron 30.0%
TCTAGGGATTTCTATGTTTA+TGG + chr1_1:16395508-16395527 Msa0010850:intron 30.0%
TCTTAGTTACTCATTCGTAA+AGG + chr1_1:16395700-16395719 Msa0010850:intron 30.0%
GATGACAAAAAAACATATGC+AGG + chr1_1:16395778-16395797 Msa0010850:intron 30.0%
TTTAATCGCTTCATGTGATT+TGG + chr1_1:16395806-16395825 Msa0010850:intron 30.0%
TTTGGTAGAGATATACTACA+AGG + chr1_1:16395824-16395843 Msa0010850:intron 30.0%
TCAAATCACAAAGACAATTG+GGG + chr1_1:16395882-16395901 Msa0010850:intron 30.0%
TGTTACAAATTCAACTCACT+AGG + chr1_1:16395949-16395968 Msa0010850:intron 30.0%
GTTACAAATTCAACTCACTA+GGG + chr1_1:16395950-16395969 Msa0010850:intron 30.0%
TTACAAATTCAACTCACTAG+GGG + chr1_1:16395951-16395970 Msa0010850:intron 30.0%
ACAATAATGATCCTTAGTGT+AGG + chr1_1:16395975-16395994 Msa0010850:intron 30.0%
CAATAATGATCCTTAGTGTA+GGG + chr1_1:16395976-16395995 Msa0010850:intron 30.0%
AATAATGATCCTTAGTGTAG+GGG + chr1_1:16395977-16395996 Msa0010850:intron 30.0%
GGTTAATTACATTCGCAAAT+TGG - chr1_1:16396086-16396105 None:intergenic 30.0%
TCATGAATGTAATTAACCGT+AGG + chr1_1:16396146-16396165 Msa0010850:intron 30.0%
TCAAATAACCAATAGTCCTA+CGG - chr1_1:16396165-16396184 None:intergenic 30.0%
AATAAAAACTGCTCACACAT+TGG - chr1_1:16396368-16396387 None:intergenic 30.0%
! ATGTGTGAGCAGTTTTTATT+TGG + chr1_1:16396368-16396387 Msa0010850:intron 30.0%
!!! TATTTGGTTTTGCTACTATG+AGG + chr1_1:16396384-16396403 Msa0010850:intron 30.0%
TAAATACTAAGATTAGTGGC+CGG + chr1_1:16396521-16396540 Msa0010850:intron 30.0%
TGTAAAAACATGTTATAGGC+CGG - chr1_1:16396543-16396562 None:intergenic 30.0%
CTCAAGTAATCAAGTATTAG+AGG - chr1_1:16396595-16396614 None:intergenic 30.0%
GGTTCTATAAAGGTAAATGA+AGG - chr1_1:16396627-16396646 None:intergenic 30.0%
TCCGTACCATGTATATAATA+TGG - chr1_1:16396926-16396945 None:intergenic 30.0%
CCAGGTTATTATTATGGTTT+CGG - chr1_1:16397101-16397120 None:intergenic 30.0%
TAAATTAGCCAAAGACGAAT+AGG + chr1_1:16397204-16397223 Msa0010850:intron 30.0%
CAAAATAACCTATTCGTCTT+TGG - chr1_1:16397215-16397234 None:intergenic 30.0%
!! AAGACGAATAGGTTATTTTG+TGG + chr1_1:16397215-16397234 Msa0010850:intron 30.0%
!! AGACGAATAGGTTATTTTGT+GGG + chr1_1:16397216-16397235 Msa0010850:intron 30.0%
!!! CTAGTTTTTAGGTTCCATTT+TGG + chr1_1:16397625-16397644 Msa0010850:five_prime_UTR 30.0%
GCATCAAAATTGAACACAAA+GGG - chr1_1:16397658-16397677 None:intergenic 30.0%
TGCATCAAAATTGAACACAA+AGG - chr1_1:16397659-16397678 None:intergenic 30.0%
!! TTTGTGTTCAATTTTGATGC+AGG + chr1_1:16397658-16397677 Msa0010850:exon 30.0%
!! TTGTGTTCAATTTTGATGCA+GGG + chr1_1:16397659-16397678 Msa0010850:exon 30.0%
AAATATATTCATCGACGAGA+GGG - chr1_1:16397833-16397852 None:intergenic 30.0%
AAAATATATTCATCGACGAG+AGG - chr1_1:16397834-16397853 None:intergenic 30.0%
!! CTGGTGAGTTCTATTGATTT+AGG + chr1_1:16395409-16395428 Msa0010850:intron 35.0%
!! AGGGATTTCTTATGGTTTTG+TGG + chr1_1:16395577-16395596 Msa0010850:intron 35.0%
AGTTACTCATTCGTAAAGGT+TGG + chr1_1:16395704-16395723 Msa0010850:intron 35.0%
AGAGATATACTACAAGGTGT+TGG + chr1_1:16395830-16395849 Msa0010850:intron 35.0%
!!! ATTGGGGTTTCTAGAACTTT+TGG + chr1_1:16395898-16395917 Msa0010850:intron 35.0%
!! GCAAATTGGTTTGTCTATGT+TGG - chr1_1:16396072-16396091 None:intergenic 35.0%
TTTGCGAATGTAATTAACCG+TGG + chr1_1:16396087-16396106 Msa0010850:intron 35.0%
GTAATTAACCGTAGGACTAT+TGG + chr1_1:16396154-16396173 Msa0010850:intron 35.0%
!!! ATCAGTCAATTTTTGGTGAC+TGG + chr1_1:16396217-16396236 Msa0010850:intron 35.0%
!!! TCAGTCAATTTTTGGTGACT+GGG + chr1_1:16396218-16396237 Msa0010850:intron 35.0%
AGTAATCAAGTATTAGAGGC+CGG - chr1_1:16396591-16396610 None:intergenic 35.0%
TATAAAAGCCGAAGTAGAAC+AGG - chr1_1:16396671-16396690 None:intergenic 35.0%
GAAATGCACCTGTGTATTAT+AGG - chr1_1:16396969-16396988 None:intergenic 35.0%
AACTTTCATTACATTCTGCC+AGG + chr1_1:16397027-16397046 Msa0010850:intron 35.0%
CCGAAACCATAATAATAACC+TGG + chr1_1:16397098-16397117 Msa0010850:intron 35.0%
!! GACGAATAGGTTATTTTGTG+GGG + chr1_1:16397217-16397236 Msa0010850:intron 35.0%
!! ATAGGTTATTTTGTGGGGAT+AGG + chr1_1:16397222-16397241 Msa0010850:intron 35.0%
TATTAATGGAGCAAGAGTTG+CGG + chr1_1:16397330-16397349 Msa0010850:five_prime_UTR 35.0%
AGAATATGGTTCAGATGAGT+CGG + chr1_1:16397457-16397476 Msa0010850:five_prime_UTR 35.0%
!!! TTTTTAGGTTCCATTTTGGC+TGG + chr1_1:16397629-16397648 Msa0010850:five_prime_UTR 35.0%
TATATTCCTCGCATCATGAT+TGG + chr1_1:16397725-16397744 Msa0010850:CDS 35.0%
ATATTCCTCGCATCATGATT+GGG + chr1_1:16397726-16397745 Msa0010850:CDS 35.0%
!!! TCATGATTGGGTCTTGTTTT+TGG + chr1_1:16397738-16397757 Msa0010850:CDS 35.0%
!!! CATGATTGGGTCTTGTTTTT+GGG + chr1_1:16397739-16397758 Msa0010850:CDS 35.0%
!!! ATGATTGGGTCTTGTTTTTG+GGG + chr1_1:16397740-16397759 Msa0010850:CDS 35.0%
AGTCATACACACACAAGAAG+AGG + chr1_1:16395252-16395271 Msa0010850:five_prime_UTR 40.0%
GCGAGAAAAGAAAGAAGACA+TGG + chr1_1:16395292-16395311 Msa0010850:five_prime_UTR 40.0%
! ATGGTTGTTTGCTAAGGACT+GGG - chr1_1:16395359-16395378 None:intergenic 40.0%
! TGAGAGATGGTTGTTTGCTA+AGG - chr1_1:16395365-16395384 None:intergenic 40.0%
TAGAATGAGAACAGGGGTTA+TGG - chr1_1:16395388-16395407 None:intergenic 40.0%
ACCAGTTAGAATGAGAACAG+GGG - chr1_1:16395394-16395413 None:intergenic 40.0%
CACCAGTTAGAATGAGAACA+GGG - chr1_1:16395395-16395414 None:intergenic 40.0%
TCACCAGTTAGAATGAGAAC+AGG - chr1_1:16395396-16395415 None:intergenic 40.0%
ACACCTACGTTCAGAAACTA+AGG - chr1_1:16396005-16396024 None:intergenic 40.0%
! GGTTTGTCTATGTTGGTGTT+TGG - chr1_1:16396065-16396084 None:intergenic 40.0%
CGAATGTAATTAACCGTGGA+CGG + chr1_1:16396091-16396110 Msa0010850:intron 40.0%
!!! CAGTCAATTTTTGGTGACTG+GGG + chr1_1:16396219-16396238 Msa0010850:intron 40.0%
!!! GCTGTCTGTTTTTAAGTGTG+TGG + chr1_1:16396270-16396289 Msa0010850:intron 40.0%
CGCACAGCAACAATTCAAAA+GGG - chr1_1:16396311-16396330 None:intergenic 40.0%
ACGCACAGCAACAATTCAAA+AGG - chr1_1:16396312-16396331 None:intergenic 40.0%
GCTAAACTTTGTGAAAAGGC+CGG + chr1_1:16396569-16396588 Msa0010850:intron 40.0%
TACTTGAGCCTGTTCTACTT+CGG + chr1_1:16396660-16396679 Msa0010850:intron 40.0%
TGATCGCTCCTATAATACAC+AGG + chr1_1:16396958-16396977 Msa0010850:five_prime_UTR 40.0%
TTTCTACTCTGACAGTATGC+TGG + chr1_1:16396985-16397004 Msa0010850:intron 40.0%
TTCTACTCTGACAGTATGCT+GGG + chr1_1:16396986-16397005 Msa0010850:intron 40.0%
ACAGGGCCAGGTTATTATTA+TGG - chr1_1:16397107-16397126 None:intergenic 40.0%
TTGTGGGGATAGGAATATCA+TGG + chr1_1:16397232-16397251 Msa0010850:intron 40.0%
CACAAAATGCTGCAAGACAA+AGG - chr1_1:16397303-16397322 None:intergenic 40.0%
ATTAATGGAGCAAGAGTTGC+GGG + chr1_1:16397331-16397350 Msa0010850:five_prime_UTR 40.0%
!! TTCAAGAAAGCGTTGATCGA+CGG + chr1_1:16397390-16397409 Msa0010850:five_prime_UTR 40.0%
AATTGATCGCAAGCACATCA+AGG + chr1_1:16397420-16397439 Msa0010850:five_prime_UTR 40.0%
GAACCATATTCTTCAACACC+CGG - chr1_1:16397449-16397468 None:intergenic 40.0%
CTTTCATCCTCCTCAATGTT+GGG - chr1_1:16397488-16397507 None:intergenic 40.0%
ACTTTCATCCTCCTCAATGT+TGG - chr1_1:16397489-16397508 None:intergenic 40.0%
ATGCAGGGATATGCTATTGT+CGG + chr1_1:16397674-16397693 Msa0010850:CDS 40.0%
!!! ATTGTCGGCATCTTTTTTGC+TGG + chr1_1:16397689-16397708 Msa0010850:CDS 40.0%
TTAGGTGTGCCGCATATATA+CGG + chr1_1:16397764-16397783 Msa0010850:CDS 40.0%
CAAAAGGGACCGTATATATG+CGG - chr1_1:16397776-16397795 None:intergenic 40.0%
! TCGCGGAAGAAAGTACAAAA+GGG - chr1_1:16397791-16397810 None:intergenic 40.0%
ACACACACAAGAAGAGGATG+CGG + chr1_1:16395258-16395277 Msa0010850:five_prime_UTR 45.0%
!! GCTAAGGACTGGGTATTGAA+CGG - chr1_1:16395349-16395368 None:intergenic 45.0%
! GATGGTTGTTTGCTAAGGAC+TGG - chr1_1:16395360-16395379 None:intergenic 45.0%
ACCCCTGTTCTCATTCTAAC+TGG + chr1_1:16395390-16395409 Msa0010850:five_prime_UTR 45.0%
GCACCTTAGTTTCTGAACGT+AGG + chr1_1:16395999-16396018 Msa0010850:intron 45.0%
TATATCAAGCTCTCCGTCCA+CGG - chr1_1:16396107-16396126 None:intergenic 45.0%
!!! TCAATTTTTGGTGACTGGGG+CGG + chr1_1:16396222-16396241 Msa0010850:intron 45.0%
TTGAATTGTTGCTGTGCGTG+TGG + chr1_1:16396313-16396332 Msa0010850:intron 45.0%
!!! GTGGTGGGTCTGTGATTTTT+AGG + chr1_1:16396332-16396351 Msa0010850:intron 45.0%
CGATGAAGCTCGTCTACATA+GGG - chr1_1:16396897-16396916 None:intergenic 45.0%
TCGATGAAGCTCGTCTACAT+AGG - chr1_1:16396898-16396917 None:intergenic 45.0%
TAAGCCACCAAGCTTGAACA+GGG - chr1_1:16397124-16397143 None:intergenic 45.0%
!! TGACCGGGTGTTGAAGAATA+TGG + chr1_1:16397443-16397462 Msa0010850:five_prime_UTR 45.0%
!!! TTAGGTTCCATTTTGGCTGG+TGG + chr1_1:16397632-16397651 Msa0010850:five_prime_UTR 45.0%
!!! GGCATCTTTTTTGCTGGTTG+TGG + chr1_1:16397695-16397714 Msa0010850:CDS 45.0%
!!! GGGTCTTGTTTTTGGGGTTT+AGG + chr1_1:16397746-16397765 Msa0010850:CDS 45.0%
! CTCGCGGAAGAAAGTACAAA+AGG - chr1_1:16397792-16397811 None:intergenic 45.0%
! TACTTTCTTCCGCGAGTTGA+GGG + chr1_1:16397796-16397815 Msa0010850:CDS 45.0%
!!! TTTTTATTTATTTTAGATTT+AGG + chr1_1:16395557-16395576 Msa0010850:intron 5.0%
!!! TTTTATTTATTTTAGATTTA+GGG + chr1_1:16395558-16395577 Msa0010850:intron 5.0%
AAGAAGAGGATGCGGTGAAG+AGG + chr1_1:16395266-16395285 Msa0010850:five_prime_UTR 50.0%
AGACATGGACGACATGCATC+TGG + chr1_1:16395307-16395326 Msa0010850:five_prime_UTR 50.0%
GACATGGACGACATGCATCT+GGG + chr1_1:16395308-16395327 Msa0010850:five_prime_UTR 50.0%
ACAGGGGTTATGGTGAGAGA+TGG - chr1_1:16395378-16395397 None:intergenic 50.0%
! ACTAAGGTGCCCCTACACTA+AGG - chr1_1:16395989-16396008 None:intergenic 50.0%
!!! CAATTTTTGGTGACTGGGGC+GGG + chr1_1:16396223-16396242 Msa0010850:intron 50.0%
AATTGTTGCTGTGCGTGTGG+TGG + chr1_1:16396316-16396335 Msa0010850:intron 50.0%
ATTGTTGCTGTGCGTGTGGT+GGG + chr1_1:16396317-16396336 Msa0010850:intron 50.0%
CTAAGCCACCAAGCTTGAAC+AGG - chr1_1:16397125-16397144 None:intergenic 50.0%
AGTTGCGGGACAAGATGGAA+CGG + chr1_1:16397345-16397364 Msa0010850:five_prime_UTR 50.0%
!! AAGCGTTGATCGACGGTTTG+AGG + chr1_1:16397397-16397416 Msa0010850:five_prime_UTR 50.0%
GTCGGACAATCCCAACATTG+AGG + chr1_1:16397475-16397494 Msa0010850:five_prime_UTR 50.0%
GGACAATCCCAACATTGAGG+AGG + chr1_1:16397478-16397497 Msa0010850:five_prime_UTR 50.0%
CAAGACCCAATCATGATGCG+AGG - chr1_1:16397734-16397753 None:intergenic 50.0%
GTACTTTCTTCCGCGAGTTG+AGG + chr1_1:16397795-16397814 Msa0010850:CDS 50.0%
TAAGACACACCCTCAACTCG+CGG - chr1_1:16397808-16397827 None:intergenic 50.0%
!! GCGGGTATTGGCTCTGTTTG+TGG + chr1_1:16396241-16396260 Msa0010850:intron 55.0%
AACCTGGCCCTGTTCAAGCT+TGG + chr1_1:16397114-16397133 Msa0010850:intron 55.0%
GCAAGAGTTGCGGGACAAGA+TGG + chr1_1:16397340-16397359 Msa0010850:five_prime_UTR 55.0%
GTTGCGGGACAAGATGGAAC+GGG + chr1_1:16397346-16397365 Msa0010850:five_prime_UTR 55.0%
CGCAAGCACATCAAGGTGAC+CGG + chr1_1:16397427-16397446 Msa0010850:five_prime_UTR 55.0%
GCAAGCACATCAAGGTGACC+GGG + chr1_1:16397428-16397447 Msa0010850:five_prime_UTR 55.0%
CAAAGGGCCACCAGCCAAAA+TGG - chr1_1:16397642-16397661 None:intergenic 55.0%
! TTGGTGACTGGGGCGGGTAT+TGG + chr1_1:16396229-16396248 Msa0010850:intron 60.0%
CACCAAGCTTGAACAGGGCC+AGG - chr1_1:16397119-16397138 None:intergenic 60.0%
CTGGCCCTGTTCAAGCTTGG+TGG + chr1_1:16397117-16397136 Msa0010850:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 16395229 16397867 16395229 ID=Msa0010850;Name=Msa0010850
chr1_1 mRNA 16395229 16397867 16395229 ID=Msa0010850-mRNA-1;Parent=Msa0010850;Name=Msa0010850-mRNA-1;_AED=0.33;_eAED=0.33;_QI=618|1|1|1|0.66|0.5|4|23|56
chr1_1 exon 16395229 16395411 16395229 ID=Msa0010850-mRNA-1:exon:6008;Parent=Msa0010850-mRNA-1
chr1_1 exon 16396873 16396979 16396873 ID=Msa0010850-mRNA-1:exon:6009;Parent=Msa0010850-mRNA-1
chr1_1 exon 16397314 16397581 16397314 ID=Msa0010850-mRNA-1:exon:6010;Parent=Msa0010850-mRNA-1
chr1_1 exon 16397614 16397867 16397614 ID=Msa0010850-mRNA-1:exon:6011;Parent=Msa0010850-mRNA-1
chr1_1 five_prime_UTR 16395229 16395411 16395229 ID=Msa0010850-mRNA-1:five_prime_utr;Parent=Msa0010850-mRNA-1
chr1_1 five_prime_UTR 16396873 16396979 16396873 ID=Msa0010850-mRNA-1:five_prime_utr;Parent=Msa0010850-mRNA-1
chr1_1 five_prime_UTR 16397314 16397581 16397314 ID=Msa0010850-mRNA-1:five_prime_utr;Parent=Msa0010850-mRNA-1
chr1_1 five_prime_UTR 16397614 16397673 16397614 ID=Msa0010850-mRNA-1:five_prime_utr;Parent=Msa0010850-mRNA-1
chr1_1 CDS 16397674 16397844 16397674 ID=Msa0010850-mRNA-1:cds;Parent=Msa0010850-mRNA-1
chr1_1 three_prime_UTR 16397845 16397867 16397845 ID=Msa0010850-mRNA-1:three_prime_utr;Parent=Msa0010850-mRNA-1
Gene Sequence

>Msa0010850

ATGCAGGGATATGCTATTGTCGGCATCTTTTTTGCTGGTTGTGGCATGTTGTATATTCCTCGCATCATGATTGGGTCTTGTTTTTGGGGTTTAGGTGTGCCGCATATATACGGTCCCTTTTGTACTTTCTTCCGCGAGTTGAGGGTGTGTCTTACACCCTCTCGTCGATGA
Protein sequence

>Msa0010850

MQGYAIVGIFFAGCGMLYIPRIMIGSCFWGLGVPHIYGPFCTFFRELRVCLTPSRR*