AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0818170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTGTAATGTTAATATCAT+TGG 0.275760 5_3:+53719288 None:intergenic
CAAATCGTTGTTTAATATAG+AGG 0.372797 5_3:-53719408 Msa0818170:CDS
CGGAATCAGGTTGCCATCAT+TGG 0.378112 5_3:+53719454 None:intergenic
CAATGATGGCAACCTGATTC+CGG 0.381967 5_3:-53719453 Msa0818170:CDS
GGAAATAGATGATGTTCTAT+AGG 0.418163 5_3:-53719322 Msa0818170:three_prime_UTR
AAGAGGGTGGTCACAAATGA+TGG 0.429032 5_3:-53719488 Msa0818170:CDS
GTTGTTTAATATAGAGGAAG+TGG 0.491868 5_3:-53719402 Msa0818170:CDS
GATTCTGTTGTTGCAACTTG+AGG 0.498939 5_3:+53719376 None:intergenic
AATCAGGTTGCCATCATTGG+TGG 0.544019 5_3:+53719457 None:intergenic
GGGTGCCCTTCCACCAATGA+TGG 0.556531 5_3:-53719467 Msa0818170:CDS
AGAGCTCTGTGTGACTGAAG+AGG 0.558782 5_3:-53719343 Msa0818170:three_prime_UTR
CCAATGATATTAACATTACA+CGG 0.560805 5_3:-53719288 Msa0818170:three_prime_UTR
AGGTTGCCATCATTGGTGGA+AGG 0.587477 5_3:+53719461 None:intergenic
GGTTGCCATCATTGGTGGAA+GGG 0.589101 5_3:+53719462 None:intergenic
GTAATGTTAATATCATTGGA+TGG 0.606576 5_3:+53719292 None:intergenic
AGAGGGTGGTCACAAATGAT+GGG 0.608393 5_3:-53719487 Msa0818170:CDS
GATGGCAACCTGATTCCGGA+AGG 0.651224 5_3:-53719449 Msa0818170:CDS
AATATGAAATGACACAAACA+AGG 0.695515 5_3:-53719245 Msa0818170:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTACATTGTAATTTTTT+TGG + chr5_3:53720195-53720214 None:intergenic 10.0%
!!! TTTTTACATTGTAATTTTTT+TGG + chr5_3:53720195-53720214 None:intergenic 10.0%
!! AATTAAAAAGAAAAGGAAAA+AGG + chr5_3:53719301-53719320 None:intergenic 15.0%
!! TGTAATTTCTTTGATTAAAT+AGG - chr5_3:53719684-53719703 Msa0818170:intron 15.0%
!!! AAGTTTCTTTTTTTTTGAAA+AGG - chr5_3:53719712-53719731 Msa0818170:intron 15.0%
!! TTTGGAAAAAAAGAAATAAT+CGG + chr5_3:53720017-53720036 None:intergenic 15.0%
!! AATTAAAAAGAAAAGGAAAA+AGG + chr5_3:53719301-53719320 None:intergenic 15.0%
!! TGTAATTTCTTTGATTAAAT+AGG - chr5_3:53719684-53719703 Msa0818170:intron 15.0%
!!! AAGTTTCTTTTTTTTTGAAA+AGG - chr5_3:53719712-53719731 Msa0818170:intron 15.0%
!! TTTGGAAAAAAAGAAATAAT+CGG + chr5_3:53720017-53720036 None:intergenic 15.0%
!!! ATGATAATGATGATGAAAAA+TGG - chr5_3:53719201-53719220 Msa0818170:three_prime_UTR 20.0%
!!! TGATAATGATGATGAAAAAT+GGG - chr5_3:53719202-53719221 Msa0818170:three_prime_UTR 20.0%
!! AAATTCAATAATTATACACG+TGG - chr5_3:53719403-53719422 Msa0818170:CDS 20.0%
!!! GTACTATTATACTATCTTTT+AGG + chr5_3:53719579-53719598 None:intergenic 20.0%
!!! TGTGGTAATATATTTCATTT+TGG + chr5_3:53719738-53719757 None:intergenic 20.0%
!!! ACATTGTAATTTTTTTGGTT+CGG + chr5_3:53720190-53720209 None:intergenic 20.0%
!!! CTACTGTTTATTTCATTTTT+TGG - chr5_3:53720366-53720385 Msa0818170:intron 20.0%
!!! AATATGTATACTACGTTTTT+TGG - chr5_3:53720389-53720408 Msa0818170:intron 20.0%
!!! ATATGTATACTACGTTTTTT+GGG - chr5_3:53720390-53720409 Msa0818170:intron 20.0%
!! AAAAGTTATTGAACAATCAT+GGG + chr5_3:53720587-53720606 None:intergenic 20.0%
!! AAAAAGTTATTGAACAATCA+TGG + chr5_3:53720588-53720607 None:intergenic 20.0%
!!! TTTATGCATAAGTTGATATA+TGG + chr5_3:53720726-53720745 None:intergenic 20.0%
!!! ATGATAATGATGATGAAAAA+TGG - chr5_3:53719201-53719220 Msa0818170:three_prime_UTR 20.0%
!!! TGATAATGATGATGAAAAAT+GGG - chr5_3:53719202-53719221 Msa0818170:three_prime_UTR 20.0%
!! AAATTCAATAATTATACACG+TGG - chr5_3:53719403-53719422 Msa0818170:CDS 20.0%
!!! GTACTATTATACTATCTTTT+AGG + chr5_3:53719579-53719598 None:intergenic 20.0%
!!! TGTGGTAATATATTTCATTT+TGG + chr5_3:53719738-53719757 None:intergenic 20.0%
!!! ACATTGTAATTTTTTTGGTT+CGG + chr5_3:53720190-53720209 None:intergenic 20.0%
!!! CTACTGTTTATTTCATTTTT+TGG - chr5_3:53720366-53720385 Msa0818170:intron 20.0%
!!! AATATGTATACTACGTTTTT+TGG - chr5_3:53720389-53720408 Msa0818170:intron 20.0%
!!! ATATGTATACTACGTTTTTT+GGG - chr5_3:53720390-53720409 Msa0818170:intron 20.0%
!! AAAAGTTATTGAACAATCAT+GGG + chr5_3:53720587-53720606 None:intergenic 20.0%
!! AAAAAGTTATTGAACAATCA+TGG + chr5_3:53720588-53720607 None:intergenic 20.0%
!!! TTTATGCATAAGTTGATATA+TGG + chr5_3:53720726-53720745 None:intergenic 20.0%
!!! AAAAATGGGTTTTGATGAAA+TGG - chr5_3:53719216-53719235 Msa0818170:three_prime_UTR 25.0%
!!! AAAATGGGTTTTGATGAAAT+GGG - chr5_3:53719217-53719236 Msa0818170:three_prime_UTR 25.0%
! AAAAAGAAAAGGAAAAAGGA+AGG + chr5_3:53719297-53719316 None:intergenic 25.0%
! AAAAGGACTAAAATTGCAAT+GGG - chr5_3:53719461-53719480 Msa0818170:CDS 25.0%
!!! AAATTGTGGTTTTAGTACTT+AGG - chr5_3:53719502-53719521 Msa0818170:CDS 25.0%
!!! AATTGTGGTTTTAGTACTTA+GGG - chr5_3:53719503-53719522 Msa0818170:CDS 25.0%
!! CTTTCAAAATCTTGCAATTT+TGG + chr5_3:53719531-53719550 None:intergenic 25.0%
!!! ATTGCAAGATTTTGAAAGTT+AGG - chr5_3:53719533-53719552 Msa0818170:intron 25.0%
!!! TTGCAAGATTTTGAAAGTTA+GGG - chr5_3:53719534-53719553 Msa0818170:intron 25.0%
! AGAAATAATCGGTTATTCAA+TGG + chr5_3:53720006-53720025 None:intergenic 25.0%
!! AATCATTTTGAATTACTCCA+CGG - chr5_3:53720265-53720284 Msa0818170:intron 25.0%
!!! TATGTATACTACGTTTTTTG+GGG - chr5_3:53720391-53720410 Msa0818170:intron 25.0%
! CTATGAAACGATGATAATAA+GGG + chr5_3:53720432-53720451 None:intergenic 25.0%
! ACTATGAAACGATGATAATA+AGG + chr5_3:53720433-53720452 None:intergenic 25.0%
! TTATTATCATCGTTTCATAG+TGG - chr5_3:53720432-53720451 Msa0818170:intron 25.0%
!!! TCATGATTTTGATTTGTTGT+TGG - chr5_3:53720509-53720528 Msa0818170:intron 25.0%
! CAAATCGTTGTTTAATATAG+AGG - chr5_3:53720862-53720881 Msa0818170:intron 25.0%
! GTAATGTTAATATCATTGGA+TGG + chr5_3:53720981-53721000 None:intergenic 25.0%
! CCAATGATATTAACATTACA+CGG - chr5_3:53720982-53721001 Msa0818170:intron 25.0%
! AACATTACACGGAAAAAAAA+TGG - chr5_3:53720993-53721012 Msa0818170:intron 25.0%
! AATATGAAATGACACAAACA+AGG - chr5_3:53721025-53721044 Msa0818170:intron 25.0%
!!! AAAAATGGGTTTTGATGAAA+TGG - chr5_3:53719216-53719235 Msa0818170:three_prime_UTR 25.0%
!!! AAAATGGGTTTTGATGAAAT+GGG - chr5_3:53719217-53719236 Msa0818170:three_prime_UTR 25.0%
! AAAAAGAAAAGGAAAAAGGA+AGG + chr5_3:53719297-53719316 None:intergenic 25.0%
! AAAAGGACTAAAATTGCAAT+GGG - chr5_3:53719461-53719480 Msa0818170:CDS 25.0%
!!! AAATTGTGGTTTTAGTACTT+AGG - chr5_3:53719502-53719521 Msa0818170:CDS 25.0%
!!! AATTGTGGTTTTAGTACTTA+GGG - chr5_3:53719503-53719522 Msa0818170:CDS 25.0%
!! CTTTCAAAATCTTGCAATTT+TGG + chr5_3:53719531-53719550 None:intergenic 25.0%
!!! ATTGCAAGATTTTGAAAGTT+AGG - chr5_3:53719533-53719552 Msa0818170:intron 25.0%
!!! TTGCAAGATTTTGAAAGTTA+GGG - chr5_3:53719534-53719553 Msa0818170:intron 25.0%
! AGAAATAATCGGTTATTCAA+TGG + chr5_3:53720006-53720025 None:intergenic 25.0%
!! AATCATTTTGAATTACTCCA+CGG - chr5_3:53720265-53720284 Msa0818170:intron 25.0%
!!! TATGTATACTACGTTTTTTG+GGG - chr5_3:53720391-53720410 Msa0818170:intron 25.0%
! CTATGAAACGATGATAATAA+GGG + chr5_3:53720432-53720451 None:intergenic 25.0%
! ACTATGAAACGATGATAATA+AGG + chr5_3:53720433-53720452 None:intergenic 25.0%
! TTATTATCATCGTTTCATAG+TGG - chr5_3:53720432-53720451 Msa0818170:intron 25.0%
!!! TCATGATTTTGATTTGTTGT+TGG - chr5_3:53720509-53720528 Msa0818170:intron 25.0%
! CAAATCGTTGTTTAATATAG+AGG - chr5_3:53720862-53720881 Msa0818170:intron 25.0%
! GTAATGTTAATATCATTGGA+TGG + chr5_3:53720981-53721000 None:intergenic 25.0%
! CCAATGATATTAACATTACA+CGG - chr5_3:53720982-53721001 Msa0818170:intron 25.0%
! AACATTACACGGAAAAAAAA+TGG - chr5_3:53720993-53721012 Msa0818170:intron 25.0%
! AATATGAAATGACACAAACA+AGG - chr5_3:53721025-53721044 Msa0818170:intron 25.0%
! ATGGGTTAAGAAGAAAAGTT+TGG - chr5_3:53719235-53719254 Msa0818170:three_prime_UTR 30.0%
!! CATTGCAATTTTAGTCCTTT+TGG + chr5_3:53719462-53719481 None:intergenic 30.0%
CAAAAGGACTAAAATTGCAA+TGG - chr5_3:53719460-53719479 Msa0818170:CDS 30.0%
AAAGGACTAAAATTGCAATG+GGG - chr5_3:53719462-53719481 Msa0818170:CDS 30.0%
!!! ATTGTGGTTTTAGTACTTAG+GGG - chr5_3:53719504-53719523 Msa0818170:CDS 30.0%
!! TGCAAGATTTTGAAAGTTAG+GGG - chr5_3:53719535-53719554 Msa0818170:intron 30.0%
CACCAACCATGATAATTAAA+GGG - chr5_3:53719898-53719917 Msa0818170:intron 30.0%
TTCCCTTTAATTATCATGGT+TGG + chr5_3:53719903-53719922 None:intergenic 30.0%
TCTCTTCCCTTTAATTATCA+TGG + chr5_3:53719907-53719926 None:intergenic 30.0%
TGATAATTAAAGGGAAGAGA+AGG - chr5_3:53719907-53719926 Msa0818170:intron 30.0%
! GATCAATTTTACAGGTTCTT+TGG + chr5_3:53720059-53720078 None:intergenic 30.0%
!!! GTAATTTTTTTGGTTCGGTT+TGG + chr5_3:53720185-53720204 None:intergenic 30.0%
CTAACTAACTGAAAAAAGCA+AGG + chr5_3:53720687-53720706 None:intergenic 30.0%
!! GTGATTCATTATTTTGCAGT+GGG - chr5_3:53720749-53720768 Msa0818170:intron 30.0%
AGTGGGAAAAAACATAAAGA+GGG - chr5_3:53720766-53720785 Msa0818170:intron 30.0%
! ATGTAATCTTCTTTTCCTTC+CGG + chr5_3:53720839-53720858 None:intergenic 30.0%
GTTGTTTAATATAGAGGAAG+TGG - chr5_3:53720868-53720887 Msa0818170:intron 30.0%
GGAAATAGATGATGTTCTAT+AGG - chr5_3:53720948-53720967 Msa0818170:intron 30.0%
CCGTGTAATGTTAATATCAT+TGG + chr5_3:53720985-53721004 None:intergenic 30.0%
TACACGGAAAAAAAATGGAA+CGG - chr5_3:53720998-53721017 Msa0818170:intron 30.0%
! ATGGGTTAAGAAGAAAAGTT+TGG - chr5_3:53719235-53719254 Msa0818170:three_prime_UTR 30.0%
!! CATTGCAATTTTAGTCCTTT+TGG + chr5_3:53719462-53719481 None:intergenic 30.0%
CAAAAGGACTAAAATTGCAA+TGG - chr5_3:53719460-53719479 Msa0818170:CDS 30.0%
AAAGGACTAAAATTGCAATG+GGG - chr5_3:53719462-53719481 Msa0818170:CDS 30.0%
!!! ATTGTGGTTTTAGTACTTAG+GGG - chr5_3:53719504-53719523 Msa0818170:CDS 30.0%
!! TGCAAGATTTTGAAAGTTAG+GGG - chr5_3:53719535-53719554 Msa0818170:intron 30.0%
CACCAACCATGATAATTAAA+GGG - chr5_3:53719898-53719917 Msa0818170:intron 30.0%
TTCCCTTTAATTATCATGGT+TGG + chr5_3:53719903-53719922 None:intergenic 30.0%
TCTCTTCCCTTTAATTATCA+TGG + chr5_3:53719907-53719926 None:intergenic 30.0%
TGATAATTAAAGGGAAGAGA+AGG - chr5_3:53719907-53719926 Msa0818170:intron 30.0%
! GATCAATTTTACAGGTTCTT+TGG + chr5_3:53720059-53720078 None:intergenic 30.0%
!!! GTAATTTTTTTGGTTCGGTT+TGG + chr5_3:53720185-53720204 None:intergenic 30.0%
CTAACTAACTGAAAAAAGCA+AGG + chr5_3:53720687-53720706 None:intergenic 30.0%
!! GTGATTCATTATTTTGCAGT+GGG - chr5_3:53720749-53720768 Msa0818170:intron 30.0%
AGTGGGAAAAAACATAAAGA+GGG - chr5_3:53720766-53720785 Msa0818170:intron 30.0%
! ATGTAATCTTCTTTTCCTTC+CGG + chr5_3:53720839-53720858 None:intergenic 30.0%
GTTGTTTAATATAGAGGAAG+TGG - chr5_3:53720868-53720887 Msa0818170:intron 30.0%
GGAAATAGATGATGTTCTAT+AGG - chr5_3:53720948-53720967 Msa0818170:intron 30.0%
CCGTGTAATGTTAATATCAT+TGG + chr5_3:53720985-53721004 None:intergenic 30.0%
TACACGGAAAAAAAATGGAA+CGG - chr5_3:53720998-53721017 Msa0818170:intron 30.0%
GGTTAAGAAGAAAAGTTTGG+AGG - chr5_3:53719238-53719257 Msa0818170:three_prime_UTR 35.0%
TAAGAAGAAAAGTTTGGAGG+TGG - chr5_3:53719241-53719260 Msa0818170:three_prime_UTR 35.0%
!! TCCTTTTGGTCAATTTTGAC+CGG + chr5_3:53719448-53719467 None:intergenic 35.0%
!!! TTGTGGTTTTAGTACTTAGG+GGG - chr5_3:53719505-53719524 Msa0818170:CDS 35.0%
!! GCAAGATTTTGAAAGTTAGG+GGG - chr5_3:53719536-53719555 Msa0818170:intron 35.0%
!! CAAGATTTTGAAAGTTAGGG+GGG - chr5_3:53719537-53719556 Msa0818170:intron 35.0%
!! AAGATTTTGAAAGTTAGGGG+GGG - chr5_3:53719538-53719557 Msa0818170:intron 35.0%
AACTTTCGATTGAGTGAATG+TGG + chr5_3:53719619-53719638 None:intergenic 35.0%
AAGAACAGCGACAAAAAAGA+GGG - chr5_3:53719834-53719853 Msa0818170:intron 35.0%
CTATGCCCAAGATATTGAAA+GGG - chr5_3:53719869-53719888 Msa0818170:intron 35.0%
CTTAGCCCTTTCAATATCTT+GGG + chr5_3:53719877-53719896 None:intergenic 35.0%
CCTTTAATTATCATGGTTGG+TGG + chr5_3:53719900-53719919 None:intergenic 35.0%
CCACCAACCATGATAATTAA+AGG - chr5_3:53719897-53719916 Msa0818170:intron 35.0%
GAATTAAGCTACTCACTTTC+AGG + chr5_3:53719951-53719970 None:intergenic 35.0%
CAAACAGTGCTCAATTATAC+AGG + chr5_3:53719983-53720002 None:intergenic 35.0%
ATGCAACAACTTGGGAAATT+TGG + chr5_3:53720035-53720054 None:intergenic 35.0%
TCTTTGGTATGCAACAACTT+GGG + chr5_3:53720043-53720062 None:intergenic 35.0%
TTCTTTGGTATGCAACAACT+TGG + chr5_3:53720044-53720063 None:intergenic 35.0%
TTTACGCAATTCATTGGTGA+TGG + chr5_3:53720102-53720121 None:intergenic 35.0%
CACCAATGAATTGCGTAAAA+TGG - chr5_3:53720103-53720122 Msa0818170:intron 35.0%
! GACCATTTTACGCAATTCAT+TGG + chr5_3:53720108-53720127 None:intergenic 35.0%
TTGGTACTATATTTCCAGGT+GGG + chr5_3:53720166-53720185 None:intergenic 35.0%
TTTGGTACTATATTTCCAGG+TGG + chr5_3:53720167-53720186 None:intergenic 35.0%
TGATTCTTTGATGTGTGTTG+CGG + chr5_3:53720250-53720269 None:intergenic 35.0%
! TTGTTGGTCTATAGTACTGT+TGG - chr5_3:53720525-53720544 Msa0818170:intron 35.0%
! TACTTAAACTCGTTTTGCCT+AGG + chr5_3:53720646-53720665 None:intergenic 35.0%
!! CGTGATTCATTATTTTGCAG+TGG - chr5_3:53720748-53720767 Msa0818170:intron 35.0%
CAGTGGGAAAAAACATAAAG+AGG - chr5_3:53720765-53720784 Msa0818170:intron 35.0%
ACACGGAAAAAAAATGGAAC+GGG - chr5_3:53720999-53721018 Msa0818170:intron 35.0%
GGTTAAGAAGAAAAGTTTGG+AGG - chr5_3:53719238-53719257 Msa0818170:three_prime_UTR 35.0%
TAAGAAGAAAAGTTTGGAGG+TGG - chr5_3:53719241-53719260 Msa0818170:three_prime_UTR 35.0%
!! TCCTTTTGGTCAATTTTGAC+CGG + chr5_3:53719448-53719467 None:intergenic 35.0%
!!! TTGTGGTTTTAGTACTTAGG+GGG - chr5_3:53719505-53719524 Msa0818170:CDS 35.0%
!! GCAAGATTTTGAAAGTTAGG+GGG - chr5_3:53719536-53719555 Msa0818170:intron 35.0%
!! CAAGATTTTGAAAGTTAGGG+GGG - chr5_3:53719537-53719556 Msa0818170:intron 35.0%
!! AAGATTTTGAAAGTTAGGGG+GGG - chr5_3:53719538-53719557 Msa0818170:intron 35.0%
AACTTTCGATTGAGTGAATG+TGG + chr5_3:53719619-53719638 None:intergenic 35.0%
AAGAACAGCGACAAAAAAGA+GGG - chr5_3:53719834-53719853 Msa0818170:intron 35.0%
CTATGCCCAAGATATTGAAA+GGG - chr5_3:53719869-53719888 Msa0818170:intron 35.0%
CTTAGCCCTTTCAATATCTT+GGG + chr5_3:53719877-53719896 None:intergenic 35.0%
CCTTTAATTATCATGGTTGG+TGG + chr5_3:53719900-53719919 None:intergenic 35.0%
CCACCAACCATGATAATTAA+AGG - chr5_3:53719897-53719916 Msa0818170:intron 35.0%
GAATTAAGCTACTCACTTTC+AGG + chr5_3:53719951-53719970 None:intergenic 35.0%
CAAACAGTGCTCAATTATAC+AGG + chr5_3:53719983-53720002 None:intergenic 35.0%
ATGCAACAACTTGGGAAATT+TGG + chr5_3:53720035-53720054 None:intergenic 35.0%
TCTTTGGTATGCAACAACTT+GGG + chr5_3:53720043-53720062 None:intergenic 35.0%
TTCTTTGGTATGCAACAACT+TGG + chr5_3:53720044-53720063 None:intergenic 35.0%
TTTACGCAATTCATTGGTGA+TGG + chr5_3:53720102-53720121 None:intergenic 35.0%
CACCAATGAATTGCGTAAAA+TGG - chr5_3:53720103-53720122 Msa0818170:intron 35.0%
! GACCATTTTACGCAATTCAT+TGG + chr5_3:53720108-53720127 None:intergenic 35.0%
TTGGTACTATATTTCCAGGT+GGG + chr5_3:53720166-53720185 None:intergenic 35.0%
TTTGGTACTATATTTCCAGG+TGG + chr5_3:53720167-53720186 None:intergenic 35.0%
TGATTCTTTGATGTGTGTTG+CGG + chr5_3:53720250-53720269 None:intergenic 35.0%
! TTGTTGGTCTATAGTACTGT+TGG - chr5_3:53720525-53720544 Msa0818170:intron 35.0%
! TACTTAAACTCGTTTTGCCT+AGG + chr5_3:53720646-53720665 None:intergenic 35.0%
!! CGTGATTCATTATTTTGCAG+TGG - chr5_3:53720748-53720767 Msa0818170:intron 35.0%
CAGTGGGAAAAAACATAAAG+AGG - chr5_3:53720765-53720784 Msa0818170:intron 35.0%
ACACGGAAAAAAAATGGAAC+GGG - chr5_3:53720999-53721018 Msa0818170:intron 35.0%
ACCGGTCAAAATTGACCAAA+AGG - chr5_3:53719444-53719463 Msa0818170:CDS 40.0%
TTGCAATGGGGCTAAAACTT+AGG - chr5_3:53719474-53719493 Msa0818170:CDS 40.0%
TGCAATGGGGCTAAAACTTA+GGG - chr5_3:53719475-53719494 Msa0818170:CDS 40.0%
AAACTTAGGGGGGAAAATTG+TGG - chr5_3:53719488-53719507 Msa0818170:CDS 40.0%
TGCAAACGTCTCTCATGAAA+TGG + chr5_3:53719643-53719662 None:intergenic 40.0%
AAATAGTCCTCCCTAGCATA+AGG - chr5_3:53719760-53719779 Msa0818170:intron 40.0%
TAGACTCTGATGTCTATCCT+TGG + chr5_3:53719790-53719809 None:intergenic 40.0%
CAAGAACAGCGACAAAAAAG+AGG - chr5_3:53719833-53719852 Msa0818170:intron 40.0%
CCTTTCAATATCTTGGGCAT+AGG + chr5_3:53719871-53719890 None:intergenic 40.0%
CCTATGCCCAAGATATTGAA+AGG - chr5_3:53719868-53719887 Msa0818170:intron 40.0%
GCTTAGCCCTTTCAATATCT+TGG + chr5_3:53719878-53719897 None:intergenic 40.0%
TTAAGCTACTCACTTTCAGG+TGG + chr5_3:53719948-53719967 None:intergenic 40.0%
AAAATGGTCCCTCACTGATA+AGG - chr5_3:53720119-53720138 Msa0818170:intron 40.0%
AAATGGTCCCTCACTGATAA+GGG - chr5_3:53720120-53720139 Msa0818170:intron 40.0%
GGTAAATCCCTTATCAGTGA+GGG + chr5_3:53720130-53720149 None:intergenic 40.0%
TACTATATTTCCAGGTGGGT+GGG + chr5_3:53720162-53720181 None:intergenic 40.0%
!! CGGTTTGGTACTATATTTCC+AGG + chr5_3:53720170-53720189 None:intergenic 40.0%
ATTCTTTGATGTGTGTTGCG+GGG + chr5_3:53720248-53720267 None:intergenic 40.0%
GATTCTTTGATGTGTGTTGC+GGG + chr5_3:53720249-53720268 None:intergenic 40.0%
CATCGTTTCATAGTGGTGTT+TGG - chr5_3:53720439-53720458 Msa0818170:intron 40.0%
AATACAAGATGTGTTCCCAC+AGG + chr5_3:53720559-53720578 None:intergenic 40.0%
TTTACGAGTCGAGTTTACCT+AGG - chr5_3:53720626-53720645 Msa0818170:intron 40.0%
GGGAAAAAACATAAAGAGGG+TGG - chr5_3:53720769-53720788 Msa0818170:intron 40.0%
GATTCTGTTGTTGCAACTTG+AGG + chr5_3:53720897-53720916 None:intergenic 40.0%
ACCGGTCAAAATTGACCAAA+AGG - chr5_3:53719444-53719463 Msa0818170:CDS 40.0%
TTGCAATGGGGCTAAAACTT+AGG - chr5_3:53719474-53719493 Msa0818170:CDS 40.0%
TGCAATGGGGCTAAAACTTA+GGG - chr5_3:53719475-53719494 Msa0818170:CDS 40.0%
AAACTTAGGGGGGAAAATTG+TGG - chr5_3:53719488-53719507 Msa0818170:CDS 40.0%
TGCAAACGTCTCTCATGAAA+TGG + chr5_3:53719643-53719662 None:intergenic 40.0%
AAATAGTCCTCCCTAGCATA+AGG - chr5_3:53719760-53719779 Msa0818170:intron 40.0%
TAGACTCTGATGTCTATCCT+TGG + chr5_3:53719790-53719809 None:intergenic 40.0%
CAAGAACAGCGACAAAAAAG+AGG - chr5_3:53719833-53719852 Msa0818170:intron 40.0%
CCTTTCAATATCTTGGGCAT+AGG + chr5_3:53719871-53719890 None:intergenic 40.0%
CCTATGCCCAAGATATTGAA+AGG - chr5_3:53719868-53719887 Msa0818170:intron 40.0%
GCTTAGCCCTTTCAATATCT+TGG + chr5_3:53719878-53719897 None:intergenic 40.0%
TTAAGCTACTCACTTTCAGG+TGG + chr5_3:53719948-53719967 None:intergenic 40.0%
AAAATGGTCCCTCACTGATA+AGG - chr5_3:53720119-53720138 Msa0818170:intron 40.0%
AAATGGTCCCTCACTGATAA+GGG - chr5_3:53720120-53720139 Msa0818170:intron 40.0%
GGTAAATCCCTTATCAGTGA+GGG + chr5_3:53720130-53720149 None:intergenic 40.0%
TACTATATTTCCAGGTGGGT+GGG + chr5_3:53720162-53720181 None:intergenic 40.0%
!! CGGTTTGGTACTATATTTCC+AGG + chr5_3:53720170-53720189 None:intergenic 40.0%
ATTCTTTGATGTGTGTTGCG+GGG + chr5_3:53720248-53720267 None:intergenic 40.0%
GATTCTTTGATGTGTGTTGC+GGG + chr5_3:53720249-53720268 None:intergenic 40.0%
CATCGTTTCATAGTGGTGTT+TGG - chr5_3:53720439-53720458 Msa0818170:intron 40.0%
AATACAAGATGTGTTCCCAC+AGG + chr5_3:53720559-53720578 None:intergenic 40.0%
TTTACGAGTCGAGTTTACCT+AGG - chr5_3:53720626-53720645 Msa0818170:intron 40.0%
GGGAAAAAACATAAAGAGGG+TGG - chr5_3:53720769-53720788 Msa0818170:intron 40.0%
GATTCTGTTGTTGCAACTTG+AGG + chr5_3:53720897-53720916 None:intergenic 40.0%
GCAATGGGGCTAAAACTTAG+GGG - chr5_3:53719476-53719495 Msa0818170:CDS 45.0%
CAATGGGGCTAAAACTTAGG+GGG - chr5_3:53719477-53719496 Msa0818170:CDS 45.0%
AATGGGGCTAAAACTTAGGG+GGG - chr5_3:53719478-53719497 Msa0818170:CDS 45.0%
CTAGGGAGGACTATTTGATG+TGG + chr5_3:53719756-53719775 None:intergenic 45.0%
CCTTGGCATTCCTTATGCTA+GGG + chr5_3:53719773-53719792 None:intergenic 45.0%
TCCTTGGCATTCCTTATGCT+AGG + chr5_3:53719774-53719793 None:intergenic 45.0%
! ACTCACTTTCAGGTGGTTGA+AGG + chr5_3:53719941-53719960 None:intergenic 45.0%
! GGCACGTCGATCAATTTTAC+AGG + chr5_3:53720067-53720086 None:intergenic 45.0%
AATTGATCGACGTGCCTTTG+TGG - chr5_3:53720071-53720090 Msa0818170:intron 45.0%
!! ACGCAATTCATTGGTGATGG+TGG + chr5_3:53720099-53720118 None:intergenic 45.0%
CGGTAAATCCCTTATCAGTG+AGG + chr5_3:53720131-53720150 None:intergenic 45.0%
GTACTATATTTCCAGGTGGG+TGG + chr5_3:53720163-53720182 None:intergenic 45.0%
GAATTACTCCACGGCGAAAA+AGG - chr5_3:53720274-53720293 Msa0818170:intron 45.0%
!! CTTTGTTACCTTTTTCGCCG+TGG + chr5_3:53720285-53720304 None:intergenic 45.0%
AAGAGGGTGGTCACAAATGA+TGG - chr5_3:53720782-53720801 Msa0818170:intron 45.0%
AGAGGGTGGTCACAAATGAT+GGG - chr5_3:53720783-53720802 Msa0818170:intron 45.0%
! AATCAGGTTGCCATCATTGG+TGG + chr5_3:53720816-53720835 None:intergenic 45.0%
CAATGATGGCAACCTGATTC+CGG - chr5_3:53720817-53720836 Msa0818170:intron 45.0%
! CTTCTTTTCCTTCCGGAATC+AGG + chr5_3:53720832-53720851 None:intergenic 45.0%
GCAATGGGGCTAAAACTTAG+GGG - chr5_3:53719476-53719495 Msa0818170:CDS 45.0%
CAATGGGGCTAAAACTTAGG+GGG - chr5_3:53719477-53719496 Msa0818170:CDS 45.0%
AATGGGGCTAAAACTTAGGG+GGG - chr5_3:53719478-53719497 Msa0818170:CDS 45.0%
CTAGGGAGGACTATTTGATG+TGG + chr5_3:53719756-53719775 None:intergenic 45.0%
CCTTGGCATTCCTTATGCTA+GGG + chr5_3:53719773-53719792 None:intergenic 45.0%
TCCTTGGCATTCCTTATGCT+AGG + chr5_3:53719774-53719793 None:intergenic 45.0%
! ACTCACTTTCAGGTGGTTGA+AGG + chr5_3:53719941-53719960 None:intergenic 45.0%
! GGCACGTCGATCAATTTTAC+AGG + chr5_3:53720067-53720086 None:intergenic 45.0%
AATTGATCGACGTGCCTTTG+TGG - chr5_3:53720071-53720090 Msa0818170:intron 45.0%
!! ACGCAATTCATTGGTGATGG+TGG + chr5_3:53720099-53720118 None:intergenic 45.0%
CGGTAAATCCCTTATCAGTG+AGG + chr5_3:53720131-53720150 None:intergenic 45.0%
GTACTATATTTCCAGGTGGG+TGG + chr5_3:53720163-53720182 None:intergenic 45.0%
GAATTACTCCACGGCGAAAA+AGG - chr5_3:53720274-53720293 Msa0818170:intron 45.0%
!! CTTTGTTACCTTTTTCGCCG+TGG + chr5_3:53720285-53720304 None:intergenic 45.0%
AAGAGGGTGGTCACAAATGA+TGG - chr5_3:53720782-53720801 Msa0818170:intron 45.0%
AGAGGGTGGTCACAAATGAT+GGG - chr5_3:53720783-53720802 Msa0818170:intron 45.0%
! AATCAGGTTGCCATCATTGG+TGG + chr5_3:53720816-53720835 None:intergenic 45.0%
CAATGATGGCAACCTGATTC+CGG - chr5_3:53720817-53720836 Msa0818170:intron 45.0%
! CTTCTTTTCCTTCCGGAATC+AGG + chr5_3:53720832-53720851 None:intergenic 45.0%
!! AAATTAAAATTAAAAAGAAA+AGG + chr5_3:53719308-53719327 None:intergenic 5.0%
!! AAATTAAAATTAAAAAGAAA+AGG + chr5_3:53719308-53719327 None:intergenic 5.0%
TTGACCGGTCAATGCTTACG+TGG + chr5_3:53719433-53719452 None:intergenic 50.0%
TGGCATTCCTTATGCTAGGG+AGG + chr5_3:53719770-53719789 None:intergenic 50.0%
CCCTAGCATAAGGAATGCCA+AGG - chr5_3:53719770-53719789 Msa0818170:intron 50.0%
GTGTGGGTCAGAGGAATCAT+TGG + chr5_3:53720226-53720245 None:intergenic 50.0%
ACTGTTGGAGCTTGAACCTG+TGG - chr5_3:53720540-53720559 Msa0818170:intron 50.0%
CTGTTGGAGCTTGAACCTGT+GGG - chr5_3:53720541-53720560 Msa0818170:intron 50.0%
GGTTGCCATCATTGGTGGAA+GGG + chr5_3:53720811-53720830 None:intergenic 50.0%
AGGTTGCCATCATTGGTGGA+AGG + chr5_3:53720812-53720831 None:intergenic 50.0%
! CGGAATCAGGTTGCCATCAT+TGG + chr5_3:53720819-53720838 None:intergenic 50.0%
AGAGCTCTGTGTGACTGAAG+AGG - chr5_3:53720927-53720946 Msa0818170:intron 50.0%
TTGACCGGTCAATGCTTACG+TGG + chr5_3:53719433-53719452 None:intergenic 50.0%
TGGCATTCCTTATGCTAGGG+AGG + chr5_3:53719770-53719789 None:intergenic 50.0%
CCCTAGCATAAGGAATGCCA+AGG - chr5_3:53719770-53719789 Msa0818170:intron 50.0%
GTGTGGGTCAGAGGAATCAT+TGG + chr5_3:53720226-53720245 None:intergenic 50.0%
ACTGTTGGAGCTTGAACCTG+TGG - chr5_3:53720540-53720559 Msa0818170:intron 50.0%
CTGTTGGAGCTTGAACCTGT+GGG - chr5_3:53720541-53720560 Msa0818170:intron 50.0%
GGTTGCCATCATTGGTGGAA+GGG + chr5_3:53720811-53720830 None:intergenic 50.0%
AGGTTGCCATCATTGGTGGA+AGG + chr5_3:53720812-53720831 None:intergenic 50.0%
! CGGAATCAGGTTGCCATCAT+TGG + chr5_3:53720819-53720838 None:intergenic 50.0%
AGAGCTCTGTGTGACTGAAG+AGG - chr5_3:53720927-53720946 Msa0818170:intron 50.0%
!! CGTGCCACGTAAGCATTGAC+CGG - chr5_3:53719426-53719445 Msa0818170:CDS 55.0%
!! TGGTGATGGTGGTGCCACAA+AGG + chr5_3:53720088-53720107 None:intergenic 55.0%
TGATGTGTGTTGCGGGGTGT+GGG + chr5_3:53720242-53720261 None:intergenic 55.0%
TTGATGTGTGTTGCGGGGTG+TGG + chr5_3:53720243-53720262 None:intergenic 55.0%
GATGGCAACCTGATTCCGGA+AGG - chr5_3:53720821-53720840 Msa0818170:intron 55.0%
!! CGTGCCACGTAAGCATTGAC+CGG - chr5_3:53719426-53719445 Msa0818170:CDS 55.0%
!! TGGTGATGGTGGTGCCACAA+AGG + chr5_3:53720088-53720107 None:intergenic 55.0%
TGATGTGTGTTGCGGGGTGT+GGG + chr5_3:53720242-53720261 None:intergenic 55.0%
TTGATGTGTGTTGCGGGGTG+TGG + chr5_3:53720243-53720262 None:intergenic 55.0%
GATGGCAACCTGATTCCGGA+AGG - chr5_3:53720821-53720840 Msa0818170:intron 55.0%
!! CAGGTGGGTGGGTTTGCTGA+CGG + chr5_3:53720151-53720170 None:intergenic 60.0%
GGGTGCCCTTCCACCAATGA+TGG - chr5_3:53720803-53720822 Msa0818170:intron 60.0%
!! CAGGTGGGTGGGTTTGCTGA+CGG + chr5_3:53720151-53720170 None:intergenic 60.0%
GGGTGCCCTTCCACCAATGA+TGG - chr5_3:53720803-53720822 Msa0818170:intron 60.0%
CGTCAGCAAACCCACCCACC+TGG - chr5_3:53720149-53720168 Msa0818170:intron 65.0%
TGTTGCGGGGTGTGGGTCAG+AGG + chr5_3:53720235-53720254 None:intergenic 65.0%
CGTCAGCAAACCCACCCACC+TGG - chr5_3:53720149-53720168 Msa0818170:intron 65.0%
TGTTGCGGGGTGTGGGTCAG+AGG + chr5_3:53720235-53720254 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 53719173 53721119 53719173 ID=Msa0818170;Name=Msa0818170
chr5_3 mRNA 53719173 53721119 53719173 ID=Msa0818170-mRNA-1;Parent=Msa0818170;Name=Msa0818170-mRNA-1;_AED=0.51;_eAED=0.51;_QI=0|0|0.5|0.5|0|0.5|2|200|76
chr5_3 exon 53719173 53719524 53719173 ID=Msa0818170-mRNA-1:exon:16254;Parent=Msa0818170-mRNA-1
chr5_3 exon 53721041 53721119 53721041 ID=Msa0818170-mRNA-1:exon:16253;Parent=Msa0818170-mRNA-1
chr5_3 CDS 53721041 53721119 53721041 ID=Msa0818170-mRNA-1:cds;Parent=Msa0818170-mRNA-1
chr5_3 CDS 53719373 53719524 53719373 ID=Msa0818170-mRNA-1:cds;Parent=Msa0818170-mRNA-1
chr5_3 three_prime_UTR 53719173 53719372 53719173 ID=Msa0818170-mRNA-1:three_prime_utr;Parent=Msa0818170-mRNA-1
Gene Sequence

>Msa0818170

ATGGATTGGGTTGCTGTTGTTGTTGTTGATGATAATGATGATGAAAAATGGGTTTTGATGAAATGGGTTAAGAAGAAAATGGGAAAAAACATAAAGAGGGTGGTCACAAATGATGGGTGCCCTTCCACCAATGATGGCAACCTGATTCCGGAAGGAAAAGAAGATTACATTTACAAATCGTTGTTTAATATAGAGGAAGTGGTAACCTCAAGTTGCAACAACAGAATCTAA
Protein sequence

>Msa0818170

MDWVAVVVVDDNDDEKWVLMKWVKKKMGKNIKRVVTNDGCPSTNDGNLIPEGKEDYIYKSLFNIEEVVTSSCNNRI*