AlfalfaGEDB | Msa0035810

M. sativa cultivar zhongmu-4 / Msa0035810

KEGG: K13680
GO: -
PFAM: PF13641.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0035810	sp\|Q9LZR3\|CSLA9_ARATH	76.608	513	117	3	17	528	21	531	0.0	832

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0035810	A0A072VNP9	99.245	530	4	0	1	530	1	530	0.0	1092

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0035810	Msa0036010	0.954592	2.088461e-112	-8.615850e-47
Msa0035810	Msa0083920	0.910471	1.804980e-82	-8.615850e-47
Msa0035810	Msa0219190	0.806089	9.745033e-50	-8.615850e-47
Msa0035810	Msa0298240	0.816011	6.888943e-52	-8.615850e-47
Msa0035810	Msa0336090	0.826912	2.090463e-54	-8.615850e-47
Msa0035810	Msa0688500	0.807878	4.076673e-50	-8.615850e-47
Msa0035810	Msa0761580	0.807226	5.606589e-50	-8.615850e-47
Msa0035810	Msa0872310	0.825240	5.222152e-54	-8.615850e-47
Msa0035810	Msa0905550	0.807300	5.407345e-50	-8.615850e-47
Msa0035810	Msa0937550	0.811236	7.742943e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0035810	MtrunA17_Chr1g0198111	99.245	530	4	0	1	530	1	530	0.0	1092
Msa0035810	MtrunA17_Chr7g0265711	76.460	531	121	2	1	528	1	530	0.0	878
Msa0035810	MtrunA17_Chr1g0179441	75.624	521	126	1	10	530	4	523	0.0	855
Msa0035810	MtrunA17_Chr1g0179421	71.785	521	146	1	10	530	4	523	0.0	813
Msa0035810	MtrunA17_Chr1g0179381	70.441	521	152	2	10	530	4	522	0.0	793
Msa0035810	MtrunA17_Chr4g0027221	67.297	529	159	3	12	527	15	542	0.0	760
Msa0035810	MtrunA17_Chr7g0264621	63.529	510	182	3	21	528	21	528	0.0	699
Msa0035810	MtrunA17_Chr1g0179391	76.106	339	81	0	139	477	1	339	0.0	565
Msa0035810	MtrunA17_Chr3g0135191	48.611	432	216	3	25	455	171	597	2.19e-146	436
Msa0035810	MtrunA17_Chr4g0000581	48.190	442	225	2	16	457	162	599	8.04e-146	435

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0035810	AT5G03760.1	76.608	513	117	3	17	528	21	531	832
Msa0035810	AT5G22740.1	71.401	521	146	3	10	528	14	533	797
Msa0035810	AT1G23480.1	68.605	516	162	0	13	528	39	554	772
Msa0035810	AT1G23480.2	68.605	516	162	0	13	528	39	554	772
Msa0035810	AT1G23480.3	70.539	482	142	0	47	528	1	482	739
Msa0035810	AT4G13410.1	64.833	509	173	2	16	524	34	536	728
Msa0035810	AT2G35650.1	66.732	514	167	2	17	530	43	552	726
Msa0035810	AT5G16190.3	67.000	500	163	2	25	524	6	503	709
Msa0035810	AT1G24070.1	63.168	524	190	3	2	524	30	551	706
Msa0035810	AT2G35650.4	68.182	484	150	2	47	530	1	480	698

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Find 134 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATACCTTGAGGTCACTA+AGG	0.552124	1_1:-64175814	None:intergenic
AAAATGTAAATGTGACCACA+TGG	0.616797	1_1:-64176664	None:intergenic
AAAATGTTATGTCAATGCAT+AGG	0.560427	1_1:+64176915	Msa0035810:CDS
AAACATAAAGTATGAGATAA+GGG	0.425945	1_1:+64175087	Msa0035810:CDS
AAAGCTGGTGCTCTTAAAGA+AGG	0.505067	1_1:+64175127	Msa0035810:CDS
AAATGAAGCTGGTGGATGGA+AGG	0.564281	1_1:+64175724	Msa0035810:CDS
AAATGTAAATGTGACCACAT+GGG	0.678994	1_1:-64176663	None:intergenic
AACAGTTATAGGTTTGTTTG+AGG	0.493816	1_1:+64176946	Msa0035810:CDS
AACCAACTTCCTGTTCCACA+AGG	0.645217	1_1:-64175535	None:intergenic
AACCAGAGAAACATTATAAA+TGG	0.193489	1_1:+64172882	Msa0035810:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTGCCAAATGAAATTCATT+TGG	-	chr1_1:64172730-64172749	None:intergenic	30.0%
TCCCATTTATAATGTTTCTC+TGG	-	chr1_1:64172887-64172906	None:intergenic	30.0%
AGAGAAACATTATAAATGGG+AGG	+	chr1_1:64172886-64172905	Msa0035810:CDS	30.0%
AAAAAACAGAAGTAGGTTGT+TGG	+	chr1_1:64173126-64173145	Msa0035810:intron	30.0%
CATTACTTATGATAAGGAGA+GGG	-	chr1_1:64173390-64173409	None:intergenic	30.0%
TCATTACTTATGATAAGGAG+AGG	-	chr1_1:64173391-64173410	None:intergenic	30.0%
ATAGTTTGAATCTCATTGTC+TGG	+	chr1_1:64173578-64173597	Msa0035810:intron	30.0%
AATATCAGCAAATGGTGAAT+TGG	-	chr1_1:64173721-64173740	None:intergenic	30.0%
TACCATAGTACTATTGGTAA+TGG	-	chr1_1:64173928-64173947	None:intergenic	30.0%
GATTACTACCATAGTACTAT+TGG	-	chr1_1:64173934-64173953	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	64172660	64177570	64172660	ID=Msa0035810;Name=Msa0035810
chr1_1	mRNA	64172660	64177570	64172660	ID=Msa0035810-mRNA-1;Parent=Msa0035810;Name=Msa0035810-mRNA-1;_AED=0.08;_eAED=0.08;_QI=11\|1\|1\|1\|1\|1\|9\|242\|530
chr1_1	exon	64172660	64172991	64172660	ID=Msa0035810-mRNA-1:exon:20463;Parent=Msa0035810-mRNA-1
chr1_1	exon	64174829	64174927	64174829	ID=Msa0035810-mRNA-1:exon:20464;Parent=Msa0035810-mRNA-1
chr1_1	exon	64175043	64175295	64175043	ID=Msa0035810-mRNA-1:exon:20465;Parent=Msa0035810-mRNA-1
chr1_1	exon	64175477	64175587	64175477	ID=Msa0035810-mRNA-1:exon:20466;Parent=Msa0035810-mRNA-1
chr1_1	exon	64175693	64175829	64175693	ID=Msa0035810-mRNA-1:exon:20467;Parent=Msa0035810-mRNA-1
chr1_1	exon	64176375	64176488	64176375	ID=Msa0035810-mRNA-1:exon:20468;Parent=Msa0035810-mRNA-1
chr1_1	exon	64176599	64176798	64176599	ID=Msa0035810-mRNA-1:exon:20469;Parent=Msa0035810-mRNA-1
chr1_1	exon	64176880	64177074	64176880	ID=Msa0035810-mRNA-1:exon:20470;Parent=Msa0035810-mRNA-1
chr1_1	exon	64177166	64177570	64177166	ID=Msa0035810-mRNA-1:exon:20471;Parent=Msa0035810-mRNA-1
chr1_1	five_prime_UTR	64172660	64172670	64172660	ID=Msa0035810-mRNA-1:five_prime_utr;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64172671	64172991	64172671	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64174829	64174927	64174829	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64175043	64175295	64175043	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64175477	64175587	64175477	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64175693	64175829	64175693	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64176375	64176488	64176375	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64176599	64176798	64176599	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64176880	64177074	64176880	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	CDS	64177166	64177328	64177166	ID=Msa0035810-mRNA-1:cds;Parent=Msa0035810-mRNA-1
chr1_1	three_prime_UTR	64177329	64177570	64177329	ID=Msa0035810-mRNA-1:three_prime_utr;Parent=Msa0035810-mRNA-1

Gene Sequence

>Msa0035810

ATGGATAAGCTTTCATCAACAACTAGATTTGAAAGTGTGCATTATGGTATTGTTAACCAAATGAATTTCATTTGGCAACAAGCTAGGGTACCACTTATAGTTCCAGTGTTGAAATTGTTGGTAGTTTTGTGTTTGACTATGTCAGTTATGCTTTTTGTAGAAAGAGTTTATATGGGTGTAGTCATTGTCTTTCTTAAGTTGTTTGGTCATAAACCAGAGAAACATTATAAATGGGAGGCACTTAAGGATGATGATATCGAGCTTGGAAACTCTGCTTTCCCTATGGTTTTGGTGCAAATCCCAATGTATAATGAAAAGGAGGTGTATCAATTATCAATTGGAGCTGCATGTGGGTTATCATGGCCATCTGATAGAATTATTATTCAAGTTCTTGATGACTCAACAGATCCAATCATTAAGAATATGGTGGAGATAGAATGTGAGAGATGGAGAAGGAAAGGCATAAACATAAAGTATGAGATAAGGGAAAATAGAAATGGTTACAAAGCTGGTGCTCTTAAAGAAGGAATGAAGCATAGCTATGTCAAACTTTGTGACTATGTTGCCATCTTTGATGCTGATTTTCAACCTGAATCTAACTTTCTTTACCGCACCATCCCATTCCTTGATCAAAATCCACAAGTTGGACTAGTTCAAGCTCGATGGCAATTCGTTAATGCTGATGAATGCTTAATGACGAGAATGCAAGAGATGTCATTGGACTATCATTTCCTTGTGGAACAGGAAGTTGGTTCCATAACTCATGCTTTCTTTGGTTTCAATGGTACAGCTGGTGTTTGGAGAATTTCAGCACTAAATGAAGCTGGTGGATGGAAGGACCGCACAACAGTGGAAGATATGGACTTAGCAGTACGAGCTAGTCTCAAAGGCTGGAAATTTGTGTACCTTAGTGACCTCAAGGTAAAAAGTGAATTGCCAAGTACATTCAAAGCCTACCGTTATCAGCAACACAGGTGGTCATGTGGCCCAGCAAATCTCTTCAGGAAAATGGCAATAGAAATCATAAGAAACAAGAAAGTGACCATGTGGAAGAAGTTTTATGTGATATATAGTTTTTTCTTTGTCCGGAAGATCATAGCCCATGTGGTCACATTTACATTTTACTGTGTCATTTTGCCTGCAACTGTTTTAGTTCCAGAAGTAGAAGTTCCCAAGTGGGGTGCTGTCTATATACCTTGTATTATTACACTCCTTAATGCTGTTGGAACTCCAAGGTCATTCCACTTAGTAATATTGTGGATACTTTTTGAAAATGTTATGTCAATGCATAGGACCAAGGCAACAGTTATAGGTTTGTTTGAGGCTGGTAGGGTAAATGAATGGGTAGTTACTGAGAAATTGGGTGATGCTCTTAAAATAAAATCAGGAGGTAAAGCAGCTAGAAAGCCTCGTATCACGATCGATGGAAGGATTCATTTCCTAGAACTTGGTGTGGGAGCCTACCTCTTTTTGTGTGGATGCTATGATCTTGCCTTTGGGAAGAATCAGTGCTATATATATCTTTTCCTGCAATCTATTGCCTTCTTTGTTGTGGGGGTTGGCTATGTTGGCATCTATGTTCCCAGATCTTAA

Protein sequence

>Msa0035810

MDKLSSTTRFESVHYGIVNQMNFIWQQARVPLIVPVLKLLVVLCLTMSVMLFVERVYMGVVIVFLKLFGHKPEKHYKWEALKDDDIELGNSAFPMVLVQIPMYNEKEVYQLSIGAACGLSWPSDRIIIQVLDDSTDPIIKNMVEIECERWRRKGINIKYEIRENRNGYKAGALKEGMKHSYVKLCDYVAIFDADFQPESNFLYRTIPFLDQNPQVGLVQARWQFVNADECLMTRMQEMSLDYHFLVEQEVGSITHAFFGFNGTAGVWRISALNEAGGWKDRTTVEDMDLAVRASLKGWKFVYLSDLKVKSELPSTFKAYRYQQHRWSCGPANLFRKMAIEIIRNKKVTMWKKFYVIYSFFFVRKIIAHVVTFTFYCVILPATVLVPEVEVPKWGAVYIPCIITLLNAVGTPRSFHLVILWILFENVMSMHRTKATVIGLFEAGRVNEWVVTEKLGDALKIKSGGKAARKPRITIDGRIHFLELGVGAYLFLCGCYDLAFGKNQCYIYLFLQSIAFFVVGVGYVGIYVPRS*