AlfalfaGEDB | Msa0937550

M. sativa cultivar zhongmu-4 / Msa0937550

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0937550	sp\|Q9LXF1\|MYB16_ARATH	55.026	378	97	8	1	357	1	326	1.33e-127	372

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0937550	G7KKX7	97.207	358	8	2	1	357	1	357	0.0	707

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TFs/TRs:

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Gene ID	Type	Classification
Msa0937550	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001950	Msa0937550	0.804037	2.618248e-49	-8.615850e-47
Msa0002380	Msa0937550	0.802095	6.599406e-49	-8.615850e-47
Msa0002930	Msa0937550	0.823017	1.736975e-53	-8.615850e-47
Msa0003470	Msa0937550	0.820965	5.189734e-53	-8.615850e-47
Msa0004360	Msa0937550	0.866092	3.671515e-65	-8.615850e-47
Msa0005050	Msa0937550	0.899023	2.984712e-77	-8.615850e-47
Msa0005710	Msa0937550	0.805026	1.628012e-49	-8.615850e-47
Msa0008410	Msa0937550	0.819061	1.414970e-52	-8.615850e-47
Msa0012440	Msa0937550	0.834982	2.187689e-56	-8.615850e-47
Msa0016120	Msa0937550	0.824897	6.291934e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0937550	MtrunA17_Chr6g0453471	97.207	358	8	2	1	357	1	357	0.0	707
Msa0937550	MtrunA17_Chr7g0217741	60.322	373	68	10	1	357	1	309	4.48e-135	387
Msa0937550	MtrunA17_Chr4g0042671	85.799	169	24	0	1	169	1	169	1.24e-105	316
Msa0937550	MtrunA17_Chr3g0115821	77.444	133	30	0	1	133	1	133	3.76e-78	243
Msa0937550	MtrunA17_Chr8g0349261	65.714	175	52	4	1	171	1	171	9.19e-77	238
Msa0937550	MtrunA17_Chr4g0039971	63.314	169	56	2	1	163	2	170	1.74e-74	234
Msa0937550	MtrunA17_Chr2g0281071	76.154	130	30	1	1	129	1	130	3.40e-74	233
Msa0937550	MtrunA17_Chr4g0048511	73.188	138	36	1	1	137	1	138	4.31e-74	233
Msa0937550	MtrunA17_Chr2g0323411	72.263	137	38	0	1	137	1	137	5.45e-72	227
Msa0937550	MtrunA17_Chr5g0410961	71.756	131	37	0	1	131	1	131	8.75e-70	222

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0937550	AT5G15310.1	55.026	378	97	8	1	357	1	326	1.35e-128	372
Msa0937550	AT5G15310.2	57.263	358	109	9	1	357	1	315	2.11e-124	360
Msa0937550	AT5G15310.4	87.719	171	19	1	1	171	1	169	5.38e-107	313
Msa0937550	AT3G61250.1	67.630	173	52	1	1	173	1	169	7.12e-83	254
Msa0937550	AT1G34670.1	78.519	135	29	0	1	135	1	135	2.75e-78	244
Msa0937550	AT3G02940.2	77.444	133	30	0	1	133	1	133	1.51e-77	241
Msa0937550	AT3G02940.1	77.444	133	30	0	1	133	1	133	3.83e-77	240
Msa0937550	AT3G02940.3	77.444	133	30	0	1	133	1	133	3.83e-77	240
Msa0937550	AT4G21440.1	70.199	151	45	0	1	151	1	151	2.58e-76	239
Msa0937550	AT4G05100.1	77.692	130	28	1	1	129	1	130	7.58e-75	234

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Find 68 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATAAAATAAAACTCG+CGG	0.648623	6_3:+8939499	None:intergenic
AAACCAACTTTGTCACAACA+TGG	0.572673	6_3:+8942011	None:intergenic
AACTTATTGCAGAATTGTAG+AGG	0.577508	6_3:-8939455	Msa0937550:three_prime_UTR
AACTTTCCTCTCTTAATATC+TGG	0.287644	6_3:+8941571	None:intergenic
AAGAACATGGTCATGGAAGT+TGG	0.505038	6_3:-8941935	Msa0937550:CDS
AATAGTGGAGAAATTGGAGG+AGG	0.518144	6_3:-8940111	Msa0937550:CDS
AATGGTGGTGATGGTGGAAA+TGG	0.425976	6_3:-8939775	Msa0937550:CDS
ACAATTTCATACACATTTCT+AGG	0.186226	6_3:+8939207	None:intergenic
ACTAAGATTTGAAGCAGTTT+TGG	0.252752	6_3:+8940308	None:intergenic
ACTAGAAACAATGCACTAAG+TGG	0.534934	6_3:+8939252	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTGTTAAACTCACTACAAAA+TGG	-	chr6_3:8939283-8939302	Msa0937550:three_prime_UTR	30.0%
TATATTGAAGAACATGGTCA+TGG	-	chr6_3:8939383-8939402	Msa0937550:three_prime_UTR	30.0%
TCAGAAAGAAATTGGAAAGT+TGG	-	chr6_3:8939524-8939543	Msa0937550:three_prime_UTR	30.0%
AACTTTCCTCTCTTAATATC+TGG	+	chr6_3:8939757-8939776	None:intergenic	30.0%
CAATTGAAGCAAATGAGTTA+AGG	+	chr6_3:8939904-8939923	None:intergenic	30.0%
CTTAACTCATTTGCTTCAAT+TGG	-	chr6_3:8939902-8939921	Msa0937550:CDS	30.0%
GTAGAATGGAAAAAATTGAG+GGG	+	chr6_3:8940236-8940255	None:intergenic	30.0%
AACAAAAATGGGAATTGATC+CGG	-	chr6_3:8940940-8940959	Msa0937550:intron	30.0%
ATCATAATCACTTGAAAGCT+TGG	-	chr6_3:8941128-8941147	Msa0937550:intron	30.0%
GGAGAAAATACCAACAATAA+TGG	-	chr6_3:8941235-8941254	Msa0937550:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	8939148	8942199	8939148	ID=Msa0937550;Name=Msa0937550
chr6_3	mRNA	8939148	8942199	8939148	ID=Msa0937550-mRNA-1;Parent=Msa0937550;Name=Msa0937550-mRNA-1;_AED=0.08;_eAED=0.08;_QI=154\|1\|1\|1\|1\|1\|3\|533\|357
chr6_3	exon	8939148	8940491	8939148	ID=Msa0937550-mRNA-1:exon:2874;Parent=Msa0937550-mRNA-1
chr6_3	exon	8941517	8941646	8941517	ID=Msa0937550-mRNA-1:exon:2873;Parent=Msa0937550-mRNA-1
chr6_3	exon	8941913	8942199	8941913	ID=Msa0937550-mRNA-1:exon:2872;Parent=Msa0937550-mRNA-1
chr6_3	five_prime_UTR	8942046	8942199	8942046	ID=Msa0937550-mRNA-1:five_prime_utr;Parent=Msa0937550-mRNA-1
chr6_3	CDS	8941913	8942045	8941913	ID=Msa0937550-mRNA-1:cds;Parent=Msa0937550-mRNA-1
chr6_3	CDS	8941517	8941646	8941517	ID=Msa0937550-mRNA-1:cds;Parent=Msa0937550-mRNA-1
chr6_3	CDS	8939681	8940491	8939681	ID=Msa0937550-mRNA-1:cds;Parent=Msa0937550-mRNA-1
chr6_3	three_prime_UTR	8939148	8939680	8939148	ID=Msa0937550-mRNA-1:three_prime_utr;Parent=Msa0937550-mRNA-1

Gene Sequence

>Msa0937550

ATGGGACGTTCACCATGTTGTGACAAAGTTGGTTTAAAGAAAGGTCCATGGACTCCAGAAGAAGATCAAAAACTCTTAGCTTATATTGAAGAACATGGTCATGGAAGTTGGCGTGCTTTGCCAGCTAAAGCTGGGCTTCAAAGATGTGGTAAGAGCTGCAGATTAAGATGGACTAATTATCTTAGGCCAGATATTAAGAGAGGAAAGTTTAGTTTGCAAGAAGAACAAACCATCATTCAACTTCATGCTCTCTTAGGGAACAGGTGGTCAGCTATAGCAACACATTTGGCAAAGAGAACAGATAATGAGATAAAGAATTATTGGAACACACATCTTAAGAAAAGGTTAACAAAAATGGGAATTGATCCGGTTACACACAAACCTAAAAATGATGCACTTCTTTCATCAAGTGATAATGCTCATTCCAAAACTGCTTCAAATCTTAGTCACATGGCTCAATGGGAAAGTGCTAGACTTGAAGCTGAAGCTAGACTTGTTAGAGAGTCCAAAATTCGTTCACATAATTCACTTCTTCATCATAATCACTTGAAAGCTTGGAATATTAATCTTGAGTCTCCTACTTCAACTTTATCATTCAATGAGAATGTTGCACCAATTATGAATAGTGGAGAAATTGGAGGAGGAGAAAATACCAACAATAATGGTAATGAGAAAAATATTAATAATGATGAGAACAATGGTGTTGTGTCTATGATTGAGTTTGTAGGGACTAATTCAAGTTCAATTGTGAAAGAAGAAGGTGGTGATGATCAAGATCAAGATCAATGGAAAGGTTATGAAAGTTCATTGACTTTTACATCAAATCTTCATCATGAGTTGACTATGTCAATGGATCAAAGTGTTGATGATGTCATTGCTGAAGAAGGGTTTACTAATCTTTTGCTTAAGACAAATTCTGAAGACTTGAGTTTGTCAGAGAGTGGAGGTGAATCTAACAATGGTGGTGATGGTGGAAATGGAAGTGGAAGTGAATTCTATGAAGATAACAATAACTACTGGAATAATATTCTTAATTTAGTTAATTCTTCTCCATCTGATTCACCAATGTTTTGA

Protein sequence

>Msa0937550

MGRSPCCDKVGLKKGPWTPEEDQKLLAYIEEHGHGSWRALPAKAGLQRCGKSCRLRWTNYLRPDIKRGKFSLQEEQTIIQLHALLGNRWSAIATHLAKRTDNEIKNYWNTHLKKRLTKMGIDPVTHKPKNDALLSSSDNAHSKTASNLSHMAQWESARLEAEARLVRESKIRSHNSLLHHNHLKAWNINLESPTSTLSFNENVAPIMNSGEIGGGENTNNNGNEKNINNDENNGVVSMIEFVGTNSSSIVKEEGGDDQDQDQWKGYESSLTFTSNLHHELTMSMDQSVDDVIAEEGFTNLLLKTNSEDLSLSESGGESNNGGDGGNGSGSEFYEDNNNYWNNILNLVNSSPSDSPMF*