AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0087220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087220 MtrunA17_Chr1g0203711 96.113 283 10 1 1 282 159 441 0.0 561
Msa0087220 MtrunA17_Chr6g0472551 64.784 301 82 10 1 282 161 456 1.30e-115 340
Msa0087220 MtrunA17_Chr4g0057321 34.981 263 136 10 2 242 133 382 1.40e-24 102
Msa0087220 MtrunA17_Chr1g0166431 52.564 78 32 2 167 242 292 366 5.61e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087220 AT5G07240.1 34.909 275 109 9 1 264 150 365 8.50e-24 100
Msa0087220 AT5G07240.2 34.909 275 109 9 1 264 196 411 1.01e-23 100
Msa0087220 AT5G62070.1 38.911 257 89 13 1 248 161 358 3.51e-21 92.8

Find 89 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTTGCTAATTCATTTA+TGG 0.163842 1_2:+68124094 None:intergenic
TCAGTGTTTGCCATGTAATT+AGG 0.191553 1_2:+68121503 None:intergenic
TTTAGTCATAAAAGGAAATT+TGG 0.214044 1_2:-68124053 Msa0087220:five_prime_UTR
ACAAGAAAGCCCCACATATT+TGG 0.215538 1_2:-68124173 Msa0087220:five_prime_UTR
GGATTGGAAGTAGCAATTTA+AGG 0.254208 1_2:-68121307 Msa0087220:CDS
TGGCAAACACTGAATCTTCT+AGG 0.269467 1_2:-68121493 Msa0087220:CDS
GAGTGTTCATGGAGTTTCTT+AGG 0.278838 1_2:-68121542 Msa0087220:CDS
TTATATGAGGCAAACTAAAA+TGG 0.302317 1_2:-68123979 Msa0087220:five_prime_UTR
GAAGTTGCAAGCACTGGTTC+GGG 0.327277 1_2:-68122767 Msa0087220:five_prime_UTR
AATCTTCCTAAAATTTGAAT+TGG 0.331141 1_2:+68121937 None:intergenic
CAGTGTTTGCCATGTAATTA+GGG 0.331564 1_2:+68121504 None:intergenic
TGTGGTTGCTCATATTCTTC+AGG 0.331662 1_2:+68122274 None:intergenic
TGAAGTTGCAAGCACTGGTT+CGG 0.362471 1_2:-68122768 Msa0087220:five_prime_UTR
CTACAAGAAGTGAGTGTTCA+TGG 0.367097 1_2:-68121553 Msa0087220:CDS
ATCAACCACGTTTGAGGTAA+TGG 0.380501 1_2:-68123929 Msa0087220:five_prime_UTR
AGGAATATAGTTCTACTAGA+AGG 0.389996 1_2:-68121427 Msa0087220:CDS
CATGAACACTCACTTCTTGT+AGG 0.394316 1_2:+68121554 None:intergenic
TCACTTCTTGTAGGTGTAAA+GGG 0.394452 1_2:+68121563 None:intergenic
CAACCCTACCTACTAGTACT+TGG 0.395903 1_2:+68123904 None:intergenic
CTTTGCTTTGCCAATTCAAA+AGG 0.400510 1_2:-68123771 Msa0087220:intron
CTGATGAACATAAAATTGAT+AGG 0.400812 1_2:-68121814 Msa0087220:CDS
ATGATTTGTAAATCATGAAT+TGG 0.402034 1_2:-68121052 Msa0087220:three_prime_UTR
GTTGAGAGCACTTAAAGCTT+TGG 0.405805 1_2:-68122791 Msa0087220:five_prime_UTR
ATGATAGTTACCTTTATATG+AGG 0.412562 1_2:-68123992 Msa0087220:five_prime_UTR
CATGTCTATAGTACAAAATT+CGG 0.414346 1_2:-68122250 Msa0087220:CDS
CATCCAATGGTCTAACCAAT+TGG 0.414937 1_2:+68121889 None:intergenic
GAAGAAGCAGAAAATGTTTG+TGG 0.415494 1_2:+68121617 None:intergenic
TTTCAAGGTCTTTGGGAAGT+GGG 0.434323 1_2:-68121401 Msa0087220:CDS
CAGACAATGATTCTGATTCA+AGG 0.437973 1_2:-68121367 Msa0087220:CDS
ACCTCAAACGTGGTTGATGA+TGG 0.442254 1_2:+68123934 None:intergenic
TCCTCAAAATGCCTTTGTCT+TGG 0.443738 1_2:+68121446 None:intergenic
CTCACTTCTTGTAGGTGTAA+AGG 0.446179 1_2:+68121562 None:intergenic
AACCCTACCTACTAGTACTT+GGG 0.446258 1_2:+68123905 None:intergenic
GTGGTTGCTCATATTCTTCA+GGG 0.446728 1_2:+68122275 None:intergenic
GCTCCAATTGGTTAGACCAT+TGG 0.447342 1_2:-68121892 Msa0087220:CDS
ATGTAATGTGATAATGTAAT+AGG 0.457562 1_2:-68123871 Msa0087220:five_prime_UTR
CTCTGGTTACCCTAATTACA+TGG 0.469823 1_2:-68121513 Msa0087220:CDS
CCCATCATCAACCACGTTTG+AGG 0.472310 1_2:-68123935 Msa0087220:five_prime_UTR
CTCACATCTATCCAAATATG+TGG 0.485545 1_2:+68124162 None:intergenic
CACTGAATCTTCTAGGGCTA+AGG 0.485595 1_2:-68121486 Msa0087220:CDS
TTTGGTGAAGTTGCAAGCAC+TGG 0.485657 1_2:-68122773 Msa0087220:five_prime_UTR
AACCACGGTTTGGCTCCAAT+TGG 0.486425 1_2:-68121904 Msa0087220:CDS
TTCAAGGTCTTTGGGAAGTG+GGG 0.496580 1_2:-68121400 Msa0087220:CDS
CTCCTTCGTACCAACAGATA+AGG 0.500355 1_2:-68120961 Msa0087220:three_prime_UTR
TTTGGGGTAACCTTATCTGT+TGG 0.506472 1_2:+68120951 None:intergenic
GAGTCAGAGAAACCACGGTT+TGG 0.506667 1_2:-68121914 Msa0087220:CDS
CAAACTAAAATGGCCCTTTG+TGG 0.516173 1_2:-68123969 Msa0087220:five_prime_UTR
ATCTGTTGGTACGAAGGAGC+TGG 0.528030 1_2:+68120965 None:intergenic
ACTTCCCAAAGACCTTGAAA+AGG 0.531733 1_2:+68121404 None:intergenic
AGTTTCTTAGGTGGATACTC+TGG 0.536101 1_2:-68121530 Msa0087220:CDS
TAGGAGATTGAAGAGCAAGT+TGG 0.536705 1_2:-68123852 Msa0087220:five_prime_UTR
TAATGGCCCCAAGTACTAGT+AGG 0.540040 1_2:-68123912 Msa0087220:five_prime_UTR
AGAAAACTGCAGATATGCTC+AGG 0.549370 1_2:-68122729 Msa0087220:exon
GGAAATTTGGTAGTCTTGTG+AGG 0.549957 1_2:-68124040 Msa0087220:five_prime_UTR
GCCCCAAGTACTAGTAGGTA+GGG 0.550581 1_2:-68123907 Msa0087220:five_prime_UTR
AGAATACACTACAAAAGTAC+AGG 0.551084 1_2:+68121129 None:intergenic
GCTTCTTCAAGAAATGGAAG+TGG 0.558785 1_2:-68121602 Msa0087220:CDS
CGGAGTTCTCTTGCATCCAA+TGG 0.559936 1_2:+68121876 None:intergenic
AAGTCGTGTGCACTTGTCAT+TGG 0.561705 1_2:-68122665 Msa0087220:CDS
GAAATTTGGTAGTCTTGTGA+GGG 0.569715 1_2:-68124039 Msa0087220:five_prime_UTR
TCACATCTATCCAAATATGT+GGG 0.573623 1_2:+68124163 None:intergenic
GGCAAACACTGAATCTTCTA+GGG 0.574429 1_2:-68121492 Msa0087220:CDS
CATAACTCTTTGTCTAGAGG+TGG 0.575397 1_2:+68124012 None:intergenic
ATAGTACTGTACCTTGAGGA+TGG 0.576723 1_2:+68122219 None:intergenic
GTCTATAGTACAAAATTCGG+TGG 0.582232 1_2:-68122247 Msa0087220:CDS
CCTCAAACGTGGTTGATGAT+GGG 0.586887 1_2:+68123935 None:intergenic
CTATTAAAACGACTCAAAGC+AGG 0.587404 1_2:+68121329 None:intergenic
CGGTGGATCTTCCATCCTCA+AGG 0.592734 1_2:-68122230 Msa0087220:intron
GGATTCTCGAAGTAGATACA+TGG 0.594900 1_2:-68121793 Msa0087220:CDS
AACCTTATCTGTTGGTACGA+AGG 0.597323 1_2:+68120959 None:intergenic
GGCCCCAAGTACTAGTAGGT+AGG 0.597381 1_2:-68123908 Msa0087220:five_prime_UTR
CACAAAGTGCACCAAGACAA+AGG 0.608130 1_2:-68121457 Msa0087220:CDS
TGTTCATGGAGTTTCTTAGG+TGG 0.609267 1_2:-68121539 Msa0087220:CDS
TATCATAACTCTTTGTCTAG+AGG 0.614715 1_2:+68124009 None:intergenic
GCTCAGGCGTATGCAAACAT+TGG 0.619659 1_2:-68122713 Msa0087220:CDS
GGTTGGCAAAGGTCCACAAA+GGG 0.621087 1_2:+68123956 None:intergenic
AAACGTGGTTGATGATGGGT+TGG 0.621102 1_2:+68123939 None:intergenic
GGTTGATGATGGGTTGGCAA+AGG 0.624147 1_2:+68123945 None:intergenic
TTTGTACTATAGACATGATG+TGG 0.627968 1_2:+68122256 None:intergenic
CACATATTTGGATAGATGTG+AGG 0.635251 1_2:-68124161 Msa0087220:five_prime_UTR
CACATCTATCCAAATATGTG+GGG 0.647476 1_2:+68124164 None:intergenic
ACCCTACCTACTAGTACTTG+GGG 0.656134 1_2:+68123906 None:intergenic
AACCAATTGGAGCCAAACCG+TGG 0.656827 1_2:+68121902 None:intergenic
GGGTTGGCAAAGGTCCACAA+AGG 0.661222 1_2:+68123955 None:intergenic
ATTGGTGCGACTACAGACAA+AGG 0.678626 1_2:-68122695 Msa0087220:CDS
AGATTGAGTCAGAGAAACCA+CGG 0.681117 1_2:-68121919 Msa0087220:CDS
AAGTTGCAAGCACTGGTTCG+GGG 0.686816 1_2:-68122766 Msa0087220:five_prime_UTR
TGGGGCCATTACCTCAAACG+TGG 0.694849 1_2:+68123924 None:intergenic
ATTGTGAATTGTGACTCACA+TGG 0.718227 1_2:-68120994 Msa0087220:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATATTTTAGTCATAAA+AGG - chr1_2:68121182-68121201 Msa0087220:three_prime_UTR 10.0%
!! AACATTTAAATTGAATTATT+AGG - chr1_2:68122836-68122855 Msa0087220:intron 10.0%
!! AAAAAAAAAAAAAAAGCAAA+TGG + chr1_2:68124045-68124064 None:intergenic 10.0%
!!! ATTTGCTTTTTTTTTTTTTT+TGG - chr1_2:68124044-68124063 Msa0087220:five_prime_UTR 10.0%
!!! TTTGCTTTTTTTTTTTTTTT+GGG - chr1_2:68124045-68124064 Msa0087220:five_prime_UTR 10.0%
!!! ATTATTTGCTAATTCATTTA+TGG + chr1_2:68121152-68121171 None:intergenic 15.0%
!! ATAAAGAAGAAATAAAACAA+GGG + chr1_2:68121823-68121842 None:intergenic 15.0%
!! ACAAAACAAAAATGAATAAA+AGG + chr1_2:68121930-68121949 None:intergenic 15.0%
!!! TATTCATTTTTGTTTTGTTT+TGG - chr1_2:68121932-68121951 Msa0087220:CDS 15.0%
!! AGATAAAATAGTTTAAAGAA+AGG + chr1_2:68122073-68122092 None:intergenic 15.0%
!! AAATTAATCTACTTAATTGT+AGG - chr1_2:68122944-68122963 Msa0087220:intron 15.0%
!!! CATAATTTGAAGTAATTAAT+TGG + chr1_2:68123055-68123074 None:intergenic 15.0%
!! TTTAGTCATAAAAGGAAATT+TGG - chr1_2:68121190-68121209 Msa0087220:three_prime_UTR 20.0%
!!! ATGTAATGTGATAATGTAAT+AGG - chr1_2:68121372-68121391 Msa0087220:CDS 20.0%
!!! ATGTTTTTTACCTTTTGAAT+TGG + chr1_2:68121485-68121504 None:intergenic 20.0%
!! CATAAAGAAGAAATAAAACA+AGG + chr1_2:68121824-68121843 None:intergenic 20.0%
!! TTTATCATTAAAAAACCGAT+TGG - chr1_2:68121881-68121900 Msa0087220:CDS 20.0%
!!! TTTTGTTTTGGATTAGTATA+TGG - chr1_2:68121944-68121963 Msa0087220:CDS 20.0%
!! AAGTAATGTTACATAAAACT+TGG + chr1_2:68122036-68122055 None:intergenic 20.0%
!! GTTTAAAGAAAGGATAAAAA+TGG + chr1_2:68122063-68122082 None:intergenic 20.0%
!! TCTTACTCTTTGATTAAAAA+TGG + chr1_2:68122106-68122125 None:intergenic 20.0%
!!! TTTTTAATCAAAGAGTAAGA+AGG - chr1_2:68122106-68122125 Msa0087220:intron 20.0%
!!! CAACATATTTTGATGAATTT+TGG - chr1_2:68122415-68122434 Msa0087220:intron 20.0%
!!! ATATTTTGATGAATTTTGGA+AGG - chr1_2:68122419-68122438 Msa0087220:intron 20.0%
!!! ACATAATTTTGCAAAATCAT+AGG - chr1_2:68122746-68122765 Msa0087220:five_prime_UTR 20.0%
!!! AAAAGAAATTGTTTTTTGAC+TGG - chr1_2:68122875-68122894 Msa0087220:intron 20.0%
!! AGATTAATTTACAACACAAA+AGG + chr1_2:68122934-68122953 None:intergenic 20.0%
!! TCTATTTGAAGAATAAACAA+TGG - chr1_2:68123128-68123147 Msa0087220:intron 20.0%
!! AATCTTCCTAAAATTTGAAT+TGG + chr1_2:68123309-68123328 None:intergenic 20.0%
!!! TTTTTTTTTTTTTGGGTGTT+CGG - chr1_2:68124052-68124071 Msa0087220:five_prime_UTR 20.0%
!!! ATGATTTGTAAATCATGAAT+TGG - chr1_2:68124191-68124210 Msa0087220:five_prime_UTR 20.0%
! ATGATAGTTACCTTTATATG+AGG - chr1_2:68121251-68121270 Msa0087220:three_prime_UTR 25.0%
!!! TTTTAGTTTGCCTCATATAA+AGG + chr1_2:68121264-68121283 None:intergenic 25.0%
! TTATATGAGGCAAACTAAAA+TGG - chr1_2:68121264-68121283 Msa0087220:three_prime_UTR 25.0%
!!! GTAACCACTTATTTTGTTTT+TGG - chr1_2:68121413-68121432 Msa0087220:CDS 25.0%
! AAATAAAACAAGGGAATAGA+AGG + chr1_2:68121814-68121833 None:intergenic 25.0%
! AACAATCAAATTGAACCAAT+CGG + chr1_2:68121899-68121918 None:intergenic 25.0%
! TTCAATTTGATTGTTACTCT+TGG - chr1_2:68121904-68121923 Msa0087220:CDS 25.0%
!!! CTTATTTTCATAAGTTCTTC+AGG + chr1_2:68122292-68122311 None:intergenic 25.0%
!!! AAAAGAATTTGCTACTTACA+GGG + chr1_2:68122641-68122660 None:intergenic 25.0%
!!! AAAAAGAATTTGCTACTTAC+AGG + chr1_2:68122642-68122661 None:intergenic 25.0%
!! ACTGGCTTAAAAATAATGAT+TGG - chr1_2:68122893-68122912 Msa0087220:intron 25.0%
! CATGTCTATAGTACAAAATT+CGG - chr1_2:68122993-68123012 Msa0087220:intron 25.0%
! AGTAATTCTTATCACTTTGA+TGG - chr1_2:68123099-68123118 Msa0087220:intron 25.0%
! CTGATGAACATAAAATTGAT+AGG - chr1_2:68123429-68123448 Msa0087220:intron 25.0%
!!! TTCTCATTTTTATTCAACTG+TGG + chr1_2:68123479-68123498 None:intergenic 25.0%
!! TTTCTATTTTGTGGAAAAAG+TGG + chr1_2:68124008-68124027 None:intergenic 25.0%
!!! TGTCTCTTTTTTCTATTTTG+TGG + chr1_2:68124017-68124036 None:intergenic 25.0%
TCACATCTATCCAAATATGT+GGG + chr1_2:68121083-68121102 None:intergenic 30.0%
TATCATAACTCTTTGTCTAG+AGG + chr1_2:68121237-68121256 None:intergenic 30.0%
AGGTAAAAAACATTCCTTTG+TGG - chr1_2:68121492-68121511 Msa0087220:CDS 30.0%
GGTAAAAAACATTCCTTTGT+GGG - chr1_2:68121493-68121512 Msa0087220:CDS 30.0%
!!! AAAGTACTTTTTTCCCACAA+AGG + chr1_2:68121509-68121528 None:intergenic 30.0%
ACACTATAATACTAGAGAGA+GGG - chr1_2:68121606-68121625 Msa0087220:CDS 30.0%
AATTTGAGAAATGACAAGCA+AGG + chr1_2:68121738-68121757 None:intergenic 30.0%
AAAGAACAATTAGTACCATG+AGG + chr1_2:68121864-68121883 None:intergenic 30.0%
! TTGGAAGACTTGTTCAATTT+GGG + chr1_2:68122017-68122036 None:intergenic 30.0%
!!! TGTTATGTATGTGCTGTTTT+AGG - chr1_2:68122800-68122819 Msa0087220:five_prime_UTR 30.0%
AATTTACAACACAAAAGGAG+AGG + chr1_2:68122929-68122948 None:intergenic 30.0%
TTTGTACTATAGACATGATG+TGG + chr1_2:68122990-68123009 None:intergenic 30.0%
CAATAAGTTATGCATGCATT+AGG + chr1_2:68123197-68123216 None:intergenic 30.0%
! GCAGTTCCAATTCAAATTTT+AGG - chr1_2:68123300-68123319 Msa0087220:intron 30.0%
!! GCTTTTGTTGAATGTTTTGA+TGG + chr1_2:68123530-68123549 None:intergenic 30.0%
! TTTTCTGCTTCTTCAAGAAA+TGG - chr1_2:68123635-68123654 Msa0087220:intron 30.0%
! AGGAATATAGTTCTACTAGA+AGG - chr1_2:68123816-68123835 Msa0087220:five_prime_UTR 30.0%
!! TTGAGTCGTTTTAATAGGAT+TGG - chr1_2:68123920-68123939 Msa0087220:five_prime_UTR 30.0%
! AGAATACACTACAAAAGTAC+AGG + chr1_2:68124117-68124136 None:intergenic 30.0%
! ATTTTGCTTAGTAATGTGCA+TGG - chr1_2:68124221-68124240 Msa0087220:five_prime_UTR 30.0%
CACATCTATCCAAATATGTG+GGG + chr1_2:68121082-68121101 None:intergenic 35.0%
CTCACATCTATCCAAATATG+TGG + chr1_2:68121084-68121103 None:intergenic 35.0%
CACATATTTGGATAGATGTG+AGG - chr1_2:68121082-68121101 Msa0087220:three_prime_UTR 35.0%
!! GAAATTTGGTAGTCTTGTGA+GGG - chr1_2:68121204-68121223 Msa0087220:three_prime_UTR 35.0%
GCAGCCAAAAACAAAATAAG+TGG + chr1_2:68121420-68121439 None:intergenic 35.0%
!!! ATTTTGTTTTTGGCTGCGTA+TGG - chr1_2:68121423-68121442 Msa0087220:CDS 35.0%
!!! TTTTGTTTTTGGCTGCGTAT+GGG - chr1_2:68121424-68121443 Msa0087220:CDS 35.0%
CTTTGCTTTGCCAATTCAAA+AGG - chr1_2:68121472-68121491 Msa0087220:CDS 35.0%
ACATTAACGTGAAAGAAGCA+AGG - chr1_2:68121571-68121590 Msa0087220:CDS 35.0%
CACACTATAATACTAGAGAG+AGG - chr1_2:68121605-68121624 Msa0087220:CDS 35.0%
!!! GATTTTCAGCTTGTTATGCA+AGG - chr1_2:68121682-68121701 Msa0087220:CDS 35.0%
! GTTTTCTAAATGATGCCTCA+TGG - chr1_2:68121846-68121865 Msa0087220:CDS 35.0%
! TGGAAGACTTGTTCAATTTG+GGG + chr1_2:68122016-68122035 None:intergenic 35.0%
! CTTGGAAGACTTGTTCAATT+TGG + chr1_2:68122018-68122037 None:intergenic 35.0%
TCTAGTCTATTTGCACTTGT+AGG + chr1_2:68122160-68122179 None:intergenic 35.0%
!! TTGATGAATTTTGGAAGGCA+AGG - chr1_2:68122424-68122443 Msa0087220:intron 35.0%
! CAGTTTTCTTTCTCACAATG+TGG + chr1_2:68122503-68122522 None:intergenic 35.0%
! AGTGGTGTCAATAACTCATT+GGG + chr1_2:68122677-68122696 None:intergenic 35.0%
! TAGTGGTGTCAATAACTCAT+TGG + chr1_2:68122678-68122697 None:intergenic 35.0%
CACCACTAATATGTGCAATT+TGG - chr1_2:68122690-68122709 Msa0087220:CDS 35.0%
TGCCAAATTGCACATATTAG+TGG + chr1_2:68122695-68122714 None:intergenic 35.0%
!!! ATGTATGTGCTGTTTTAGGT+TGG - chr1_2:68122804-68122823 Msa0087220:five_prime_UTR 35.0%
!!! TGCTGTTTTAGGTTGGATTA+TGG - chr1_2:68122811-68122830 Msa0087220:intron 35.0%
GTCTATAGTACAAAATTCGG+TGG - chr1_2:68122996-68123015 Msa0087220:intron 35.0%
GATAATAGTACTGTACCTTG+AGG + chr1_2:68123031-68123050 None:intergenic 35.0%
AAGTTTAGCCACAAACACAT+AGG + chr1_2:68123272-68123291 None:intergenic 35.0%
! ATTCTTCGATGATGCGTTTT+TGG + chr1_2:68123402-68123421 None:intergenic 35.0%
GAAGAAGCAGAAAATGTTTG+TGG + chr1_2:68123629-68123648 None:intergenic 35.0%
TCACTTCTTGTAGGTGTAAA+GGG + chr1_2:68123683-68123702 None:intergenic 35.0%
CAGTGTTTGCCATGTAATTA+GGG + chr1_2:68123742-68123761 None:intergenic 35.0%
TCAGTGTTTGCCATGTAATT+AGG + chr1_2:68123743-68123762 None:intergenic 35.0%
CAGACAATGATTCTGATTCA+AGG - chr1_2:68123876-68123895 Msa0087220:five_prime_UTR 35.0%
CTATTAAAACGACTCAAAGC+AGG + chr1_2:68123917-68123936 None:intergenic 35.0%
!! CTGCTTTGAGTCGTTTTAAT+AGG - chr1_2:68123915-68123934 Msa0087220:five_prime_UTR 35.0%
GGATTGGAAGTAGCAATTTA+AGG - chr1_2:68123936-68123955 Msa0087220:five_prime_UTR 35.0%
ATTGTGAATTGTGACTCACA+TGG - chr1_2:68124249-68124268 Msa0087220:five_prime_UTR 35.0%
ACAAGAAAGCCCCACATATT+TGG - chr1_2:68121070-68121089 Msa0087220:three_prime_UTR 40.0%
!! GGAAATTTGGTAGTCTTGTG+AGG - chr1_2:68121203-68121222 Msa0087220:three_prime_UTR 40.0%
CATAACTCTTTGTCTAGAGG+TGG + chr1_2:68121234-68121253 None:intergenic 40.0%
CAAACTAAAATGGCCCTTTG+TGG - chr1_2:68121274-68121293 Msa0087220:three_prime_UTR 40.0%
! ATCAACCACGTTTGAGGTAA+TGG - chr1_2:68121314-68121333 Msa0087220:CDS 40.0%
AACCCTACCTACTAGTACTT+GGG + chr1_2:68121341-68121360 None:intergenic 40.0%
TAGGAGATTGAAGAGCAAGT+TGG - chr1_2:68121391-68121410 Msa0087220:CDS 40.0%
!!! TTTGTTTTTGGCTGCGTATG+GGG - chr1_2:68121425-68121444 Msa0087220:CDS 40.0%
GGGTGTACTAAAGTTCAACA+TGG + chr1_2:68121996-68122015 None:intergenic 40.0%
!! GTTGAGAGCACTTAAAGCTT+TGG - chr1_2:68122452-68122471 Msa0087220:intron 40.0%
AGAAAACTGCAGATATGCTC+AGG - chr1_2:68122514-68122533 Msa0087220:intron 40.0%
! GTGGTGTCAATAACTCATTG+GGG + chr1_2:68122676-68122695 None:intergenic 40.0%
GTGGTTGCTCATATTCTTCA+GGG + chr1_2:68122971-68122990 None:intergenic 40.0%
TGTGGTTGCTCATATTCTTC+AGG + chr1_2:68122972-68122991 None:intergenic 40.0%
ATAGTACTGTACCTTGAGGA+TGG + chr1_2:68123027-68123046 None:intergenic 40.0%
CGACTTAACCTATGTGTTTG+TGG - chr1_2:68123261-68123280 Msa0087220:intron 40.0%
AGATTGAGTCAGAGAAACCA+CGG - chr1_2:68123324-68123343 Msa0087220:intron 40.0%
CATCCAATGGTCTAACCAAT+TGG + chr1_2:68123357-68123376 None:intergenic 40.0%
GGATTCTCGAAGTAGATACA+TGG - chr1_2:68123450-68123469 Msa0087220:intron 40.0%
GCTTCTTCAAGAAATGGAAG+TGG - chr1_2:68123641-68123660 Msa0087220:intron 40.0%
CTCACTTCTTGTAGGTGTAA+AGG + chr1_2:68123684-68123703 None:intergenic 40.0%
CATGAACACTCACTTCTTGT+AGG + chr1_2:68123692-68123711 None:intergenic 40.0%
CTACAAGAAGTGAGTGTTCA+TGG - chr1_2:68123690-68123709 Msa0087220:intron 40.0%
GAGTGTTCATGGAGTTTCTT+AGG - chr1_2:68123701-68123720 Msa0087220:intron 40.0%
TGTTCATGGAGTTTCTTAGG+TGG - chr1_2:68123704-68123723 Msa0087220:intron 40.0%
AGTTTCTTAGGTGGATACTC+TGG - chr1_2:68123713-68123732 Msa0087220:intron 40.0%
CTCTGGTTACCCTAATTACA+TGG - chr1_2:68123730-68123749 Msa0087220:intron 40.0%
TGGCAAACACTGAATCTTCT+AGG - chr1_2:68123750-68123769 Msa0087220:intron 40.0%
GGCAAACACTGAATCTTCTA+GGG - chr1_2:68123751-68123770 Msa0087220:intron 40.0%
!!! ACCAAGACAAAGGCATTTTG+AGG - chr1_2:68123796-68123815 Msa0087220:five_prime_UTR 40.0%
TCCTCAAAATGCCTTTGTCT+TGG + chr1_2:68123800-68123819 None:intergenic 40.0%
! TCTACTAGAAGGCCTTTTCA+AGG - chr1_2:68123827-68123846 Msa0087220:five_prime_UTR 40.0%
!! AAGGCCTTTTCAAGGTCTTT+GGG - chr1_2:68123835-68123854 Msa0087220:five_prime_UTR 40.0%
ACTTCCCAAAGACCTTGAAA+AGG + chr1_2:68123842-68123861 None:intergenic 40.0%
!! TTTTCAAGGTCTTTGGGAAG+TGG - chr1_2:68123841-68123860 Msa0087220:five_prime_UTR 40.0%
! TTTCAAGGTCTTTGGGAAGT+GGG - chr1_2:68123842-68123861 Msa0087220:five_prime_UTR 40.0%
AACCTTATCTGTTGGTACGA+AGG + chr1_2:68124287-68124306 None:intergenic 40.0%
!! AAACGTGGTTGATGATGGGT+TGG + chr1_2:68121307-68121326 None:intergenic 45.0%
!! CCTCAAACGTGGTTGATGAT+GGG + chr1_2:68121311-68121330 None:intergenic 45.0%
!! ACCTCAAACGTGGTTGATGA+TGG + chr1_2:68121312-68121331 None:intergenic 45.0%
TAATGGCCCCAAGTACTAGT+AGG - chr1_2:68121331-68121350 Msa0087220:CDS 45.0%
ACCCTACCTACTAGTACTTG+GGG + chr1_2:68121340-68121359 None:intergenic 45.0%
CAACCCTACCTACTAGTACT+TGG + chr1_2:68121342-68121361 None:intergenic 45.0%
AATACTAGAGAGAGGGAGAG+AGG - chr1_2:68121613-68121632 Msa0087220:CDS 45.0%
!! TCAGCTTGTTATGCAAGGCA+AGG - chr1_2:68121687-68121706 Msa0087220:CDS 45.0%
!! CAGCTTGTTATGCAAGGCAA+GGG - chr1_2:68121688-68121707 Msa0087220:CDS 45.0%
TTGTTATGCAAGGCAAGGGA+AGG - chr1_2:68121692-68121711 Msa0087220:CDS 45.0%
TTTGGTGAAGTTGCAAGCAC+TGG - chr1_2:68122470-68122489 Msa0087220:intron 45.0%
!! TGAAGTTGCAAGCACTGGTT+CGG - chr1_2:68122475-68122494 Msa0087220:intron 45.0%
ATTGGTGCGACTACAGACAA+AGG - chr1_2:68122548-68122567 Msa0087220:intron 45.0%
AAGTCGTGTGCACTTGTCAT+TGG - chr1_2:68122578-68122597 Msa0087220:intron 45.0%
GCTCCAATTGGTTAGACCAT+TGG - chr1_2:68123351-68123370 Msa0087220:intron 45.0%
!!! TGCGTTTTTGGTCTGGCTAA+CGG + chr1_2:68123390-68123409 None:intergenic 45.0%
!!! TCGATGATGCGTTTTTGGTC+TGG + chr1_2:68123397-68123416 None:intergenic 45.0%
!! CACTGAATCTTCTAGGGCTA+AGG - chr1_2:68123757-68123776 Msa0087220:intron 45.0%
CACAAAGTGCACCAAGACAA+AGG - chr1_2:68123786-68123805 Msa0087220:five_prime_UTR 45.0%
!! GAAGGCCTTTTCAAGGTCTT+TGG - chr1_2:68123834-68123853 Msa0087220:five_prime_UTR 45.0%
! TTCAAGGTCTTTGGGAAGTG+GGG - chr1_2:68123843-68123862 Msa0087220:five_prime_UTR 45.0%
CTCCTTCGTACCAACAGATA+AGG - chr1_2:68124282-68124301 Msa0087220:five_prime_UTR 45.0%
GGTTGGCAAAGGTCCACAAA+GGG + chr1_2:68121290-68121309 None:intergenic 50.0%
!! GGTTGATGATGGGTTGGCAA+AGG + chr1_2:68121301-68121320 None:intergenic 50.0%
CCCATCATCAACCACGTTTG+AGG - chr1_2:68121308-68121327 Msa0087220:CDS 50.0%
GCCCCAAGTACTAGTAGGTA+GGG - chr1_2:68121336-68121355 Msa0087220:CDS 50.0%
TTATGCAAGGCAAGGGAAGG+TGG - chr1_2:68121695-68121714 Msa0087220:CDS 50.0%
!! GAAGTTGCAAGCACTGGTTC+GGG - chr1_2:68122476-68122495 Msa0087220:intron 50.0%
!! AAGTTGCAAGCACTGGTTCG+GGG - chr1_2:68122477-68122496 Msa0087220:intron 50.0%
GCTCAGGCGTATGCAAACAT+TGG - chr1_2:68122530-68122549 Msa0087220:intron 50.0%
GAGTCAGAGAAACCACGGTT+TGG - chr1_2:68123329-68123348 Msa0087220:intron 50.0%
! AACCACGGTTTGGCTCCAAT+TGG - chr1_2:68123339-68123358 Msa0087220:intron 50.0%
AACCAATTGGAGCCAAACCG+TGG + chr1_2:68123344-68123363 None:intergenic 50.0%
CGGAGTTCTCTTGCATCCAA+TGG + chr1_2:68123370-68123389 None:intergenic 50.0%
ATCTGTTGGTACGAAGGAGC+TGG + chr1_2:68124281-68124300 None:intergenic 50.0%
! GGGTTGGCAAAGGTCCACAA+AGG + chr1_2:68121291-68121310 None:intergenic 55.0%
TGGGGCCATTACCTCAAACG+TGG + chr1_2:68121322-68121341 None:intergenic 55.0%
GGCCCCAAGTACTAGTAGGT+AGG - chr1_2:68121335-68121354 Msa0087220:CDS 55.0%
CGGTGGATCTTCCATCCTCA+AGG - chr1_2:68123013-68123032 Msa0087220:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 68120955 68124310 68120955 ID=Msa0087220;Name=Msa0087220
chr1_2 mRNA 68120955 68124310 68120955 ID=Msa0087220-mRNA-1;Parent=Msa0087220;Name=Msa0087220-mRNA-1;_AED=0.12;_eAED=0.12;_QI=626|1|1|1|1|1|4|349|282
chr1_2 exon 68120955 68121969 68120955 ID=Msa0087220-mRNA-1:exon:23207;Parent=Msa0087220-mRNA-1
chr1_2 exon 68122231 68122299 68122231 ID=Msa0087220-mRNA-1:exon:23206;Parent=Msa0087220-mRNA-1
chr1_2 exon 68122624 68122824 68122624 ID=Msa0087220-mRNA-1:exon:23205;Parent=Msa0087220-mRNA-1
chr1_2 exon 68123772 68124310 68123772 ID=Msa0087220-mRNA-1:exon:23204;Parent=Msa0087220-mRNA-1
chr1_2 five_prime_UTR 68123772 68124310 68123772 ID=Msa0087220-mRNA-1:five_prime_utr;Parent=Msa0087220-mRNA-1
chr1_2 five_prime_UTR 68122738 68122824 68122738 ID=Msa0087220-mRNA-1:five_prime_utr;Parent=Msa0087220-mRNA-1
chr1_2 CDS 68122624 68122737 68122624 ID=Msa0087220-mRNA-1:cds;Parent=Msa0087220-mRNA-1
chr1_2 CDS 68122231 68122299 68122231 ID=Msa0087220-mRNA-1:cds;Parent=Msa0087220-mRNA-1
chr1_2 CDS 68121304 68121969 68121304 ID=Msa0087220-mRNA-1:cds;Parent=Msa0087220-mRNA-1
chr1_2 three_prime_UTR 68120955 68121303 68120955 ID=Msa0087220-mRNA-1:three_prime_utr;Parent=Msa0087220-mRNA-1
Gene Sequence

>Msa0087220

ATGCTCAGGCGTATGCAAACATTGGTGCGACTACAGACAAAGGCACGTGCAAGTCGTGTGCACTTGTCATTGGATAATCTGCATTCTTTCAAGTCTTCACTTTCTCATTACCCTGTCCCTGAAGAATATGAGCAACCACATCATGTCTATAGTACAAAATTCGGTGGATCTTCCATCCTCAAGAGATGCAGTTCCAATTCAAATTTTAGGAAGATTGAGTCAGAGAAACCACGGTTTGGCTCCAATTGGTTAGACCATTGGATGCAAGAGAACTCCGTTAGCCAGACCAAAAACGCATCATCGAAGAATAGACACACTGATGAACATAAAATTGATAGGATTCTCGAAGTAGATACATGGAAGCCACAGTTGAATAAAAATGAGAATAATGTGAGTTCTATGTCAAATGAGTCTCCATCAAAACATTCAACAAAAGCTCAAAATCGAAGTCTCTCATTGAAGTTTCACAAAGCAAAAGAAGAAGTAGCAGCATCGCGCACTGCTGATAATAGTCCACAAACATTTTCTGCTTCTTCAAGAAATGGAAGTGGTGTAAGAAGAAATACTCCCTTTACACCTACAAGAAGTGAGTGTTCATGGAGTTTCTTAGGTGGATACTCTGGTTACCCTAATTACATGGCAAACACTGAATCTTCTAGGGCTAAGGTTAGATCACAAAGTGCACCAAGACAAAGGCATTTTGAGGAATATAGTTCTACTAGAAGGCCTTTTCAAGGTCTTTGGGAAGTGGGGTCTACTAATTCAGACAATGATTCTGATTCAAGGAGCAACAAAGTCTCACCTGCTTTGAGTCGTTTTAATAGGATTGGAAGTAGCAATTTAAGGTGA
Protein sequence

>Msa0087220

MLRRMQTLVRLQTKARASRVHLSLDNLHSFKSSLSHYPVPEEYEQPHHVYSTKFGGSSILKRCSSNSNFRKIESEKPRFGSNWLDHWMQENSVSQTKNASSKNRHTDEHKIDRILEVDTWKPQLNKNENNVSSMSNESPSKHSTKAQNRSLSLKFHKAKEEVAASRTADNSPQTFSASSRNGSGVRRNTPFTPTRSECSWSFLGGYSGYPNYMANTESSRAKVRSQSAPRQRHFEEYSSTRRPFQGLWEVGSTNSDNDSDSRSNKVSPALSRFNRIGSSNLR*