AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0659510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659510 MtrunA17_Chr8g0384731 92.431 436 8 2 1 412 1 435 0.0 818
Msa0659510 MtrunA17_Chr3g0130741 67.290 428 100 4 24 412 19 445 0.0 576
Msa0659510 MtrunA17_Chr1g0159791 51.444 381 154 6 54 406 37 414 2.57e-128 376
Msa0659510 MtrunA17_Chr4g0010951 45.606 421 166 9 47 407 34 451 2.94e-115 344
Msa0659510 MtrunA17_Chr6g0484731 43.158 380 174 10 59 401 176 550 4.06e-89 280
Msa0659510 MtrunA17_Chr3g0078321 37.306 386 195 14 59 408 45 419 8.02e-76 241
Msa0659510 MtrunA17_Chr4g0010941 47.881 236 92 3 199 405 2 235 6.56e-72 225
Msa0659510 MtrunA17_Chr7g0221681 27.230 426 231 15 39 409 40 441 3.17e-29 119
Msa0659510 MtrunA17_Chr7g0249451 26.914 405 222 15 59 409 54 438 3.22e-28 115
Msa0659510 MtrunA17_Chr4g0053721 27.174 368 219 14 73 404 79 433 2.21e-26 111
Msa0659510 MtrunA17_Chr1g0179751 23.174 397 224 14 73 409 107 482 2.41e-26 110
Msa0659510 MtrunA17_Chr2g0320621 25.130 386 216 15 70 402 73 438 8.23e-26 108
Msa0659510 MtrunA17_Chr5g0401601 26.579 380 222 15 65 404 79 441 1.50e-25 108
Msa0659510 MtrunA17_Chr7g0265331 23.650 389 217 14 70 402 77 441 1.97e-24 105
Msa0659510 MtrunA17_Chr5g0406001 27.226 393 214 17 63 408 106 473 3.23e-24 104
Msa0659510 MtrunA17_Chr4g0027681 24.607 382 216 15 73 402 76 437 5.45e-24 103
Msa0659510 MtrunA17_Chr8g0371371 26.794 418 241 18 37 411 78 473 1.43e-22 99.8
Msa0659510 MtrunA17_Chr1g0189381 25.000 404 232 16 59 409 52 437 1.01e-21 97.1
Msa0659510 MtrunA17_Chr8g0352671 24.936 393 213 14 60 404 81 439 1.14e-21 96.7
Msa0659510 MtrunA17_Chr8g0386441 25.127 394 226 14 54 404 65 432 1.75e-21 95.9
Msa0659510 MtrunA17_Chr5g0425141 24.548 387 231 14 66 411 94 460 6.37e-21 94.7
Msa0659510 MtrunA17_Chr2g0333991 23.592 373 217 14 72 400 86 434 9.54e-20 90.9
Msa0659510 MtrunA17_Chr2g0311451 24.332 374 215 14 72 401 86 435 9.87e-20 90.9
Msa0659510 MtrunA17_Chr3g0124421 24.510 408 227 17 48 404 76 453 7.58e-19 88.6
Msa0659510 MtrunA17_Chr8g0371351 24.760 416 239 16 35 404 65 452 2.25e-18 87.0
Msa0659510 MtrunA17_Chr3g0130931 26.817 399 200 24 59 404 92 451 7.43e-18 85.1
Msa0659510 MtrunA17_Chr8g0352661 25.000 388 222 15 60 404 78 439 7.63e-18 85.1
Msa0659510 MtrunA17_Chr2g0307991 25.000 376 202 15 75 401 60 404 8.91e-18 84.7
Msa0659510 MtrunA17_Chr2g0310831 24.872 390 222 18 54 401 80 440 1.03e-17 84.7
Msa0659510 MtrunA17_Chr5g0419291 25.737 373 204 17 73 401 85 428 2.86e-17 83.2
Msa0659510 MtrunA17_Chr5g0395911 24.603 378 217 15 68 402 62 414 2.50e-15 77.4
Msa0659510 MtrunA17_Chr2g0310821 24.566 403 233 17 38 402 74 443 3.13e-15 77.0
Msa0659510 MtrunA17_Chr2g0308001 25.258 388 197 18 75 404 59 411 3.73e-15 77.0
Msa0659510 MtrunA17_Chr3g0099891 26.341 410 231 22 35 401 73 454 1.44e-14 75.1
Msa0659510 MtrunA17_Chr5g0442921 26.087 391 206 18 73 404 190 556 1.70e-14 75.1
Msa0659510 MtrunA17_Chr1g0179091 23.077 364 236 12 51 401 34 366 4.07e-14 73.2
Msa0659510 MtrunA17_Chr3g0110191 24.074 378 216 14 77 406 89 443 4.59e-14 73.6
Msa0659510 MtrunA17_Chr6g0484901 25.201 373 195 18 75 401 157 491 8.11e-14 73.2
Msa0659510 MtrunA17_Chr3g0087851 23.256 387 213 15 70 404 85 439 1.23e-13 72.4
Msa0659510 MtrunA17_Chr1g0165201 24.865 370 210 14 73 401 127 469 1.59e-13 72.0
Msa0659510 MtrunA17_Chr3g0099901 25.867 375 216 15 67 402 135 486 1.81e-13 72.0
Msa0659510 MtrunA17_Chr5g0434411 22.917 384 217 12 77 406 84 442 2.08e-13 71.6
Msa0659510 MtrunA17_Chr5g0415951 24.672 381 221 18 61 401 80 434 3.57e-13 70.9
Msa0659510 MtrunA17_Chr1g0212321 24.202 376 204 15 73 401 145 486 8.07e-13 70.1
Msa0659510 MtrunA17_Chr2g0291201 24.211 380 210 17 73 402 101 452 1.14e-12 69.3
Msa0659510 MtrunA17_Chr4g0023351 32.022 178 87 8 59 209 46 216 1.25e-12 68.2
Msa0659510 MtrunA17_Chr6g0486981 22.427 379 217 15 75 404 89 439 1.61e-12 68.9
Msa0659510 MtrunA17_Chr5g0395931 23.797 374 226 14 68 402 83 436 1.82e-12 68.6
Msa0659510 MtrunA17_Chr3g0095891 24.130 431 246 20 32 404 54 461 1.85e-12 68.6
Msa0659510 MtrunA17_Chr7g0275731 25.240 416 234 19 32 402 102 485 5.06e-12 67.4
Msa0659510 MtrunA17_Chr8g0390761 28.571 168 77 6 77 216 119 271 2.61e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659510 AT4G33490.2 58.095 420 137 7 11 404 8 414 6.07e-167 475
Msa0659510 AT4G33490.1 58.163 392 125 7 11 376 5 383 8.56e-152 436
Msa0659510 AT1G44130.1 50.263 380 157 8 56 407 30 405 9.92e-131 382
Msa0659510 AT1G77480.2 47.301 389 177 5 47 408 39 426 3.08e-124 367
Msa0659510 AT1G77480.1 47.150 386 176 5 47 405 39 423 1.15e-122 363
Msa0659510 AT1G49050.1 42.377 387 174 13 59 401 187 568 2.20e-85 271
Msa0659510 AT1G49050.2 41.892 370 170 12 74 401 29 395 1.70e-80 254
Msa0659510 AT1G49050.3 40.293 273 122 9 59 292 187 457 1.49e-51 179
Msa0659510 AT1G65240.1 26.750 400 223 14 59 409 56 434 3.06e-33 130
Msa0659510 AT5G43100.2 28.307 378 210 15 65 404 66 420 2.10e-32 128
Msa0659510 AT5G43100.1 28.307 378 210 15 65 404 66 420 8.03e-32 127
Msa0659510 AT3G02740.1 27.387 398 225 12 59 409 67 447 1.73e-31 125
Msa0659510 AT2G36670.2 25.815 399 225 13 56 402 79 458 3.34e-31 125
Msa0659510 AT1G05840.1 26.355 406 223 13 59 409 62 446 7.44e-30 120
Msa0659510 AT3G02740.2 26.684 386 221 11 69 409 4 372 3.08e-29 118
Msa0659510 AT5G22850.1 25.917 409 232 14 48 403 52 442 3.31e-29 119
Msa0659510 AT2G36670.4 25.587 383 208 12 74 402 33 392 3.81e-29 118
Msa0659510 AT2G36670.3 25.587 383 208 12 74 402 33 392 3.81e-29 118
Msa0659510 AT2G36670.1 25.495 404 225 14 56 402 79 463 4.04e-29 119
Msa0659510 AT5G36260.1 25.666 413 244 12 46 411 47 443 1.84e-28 117
Msa0659510 AT1G08210.4 25.185 405 227 13 52 402 93 475 4.78e-27 113
Msa0659510 AT1G08210.3 25.185 405 227 13 52 402 107 489 5.29e-27 113
Msa0659510 AT1G08210.1 25.185 405 227 13 52 402 59 441 5.44e-27 112
Msa0659510 AT3G50050.1 27.224 371 225 14 65 404 83 439 2.15e-25 108
Msa0659510 AT1G08210.2 25.000 432 221 17 52 402 59 468 8.37e-23 100
Msa0659510 AT3G25700.1 25.455 385 214 16 73 401 82 449 1.32e-21 96.7
Msa0659510 AT2G17760.1 24.752 404 242 15 37 401 69 449 1.39e-21 97.1
Msa0659510 AT5G10080.1 25.946 370 209 12 79 402 104 454 2.33e-21 96.7
Msa0659510 AT3G51330.3 26.260 377 212 17 72 402 98 454 4.26e-20 92.4
Msa0659510 AT3G51350.1 27.200 375 203 17 74 401 101 452 4.37e-20 92.8
Msa0659510 AT4G35880.1 27.105 380 214 17 69 407 100 457 4.40e-20 92.8
Msa0659510 AT3G51330.1 26.330 376 211 17 72 401 98 453 5.17e-20 92.4
Msa0659510 AT2G28010.1 26.371 383 227 18 47 404 42 394 6.83e-20 91.3
Msa0659510 AT2G28030.1 23.467 375 236 15 54 404 43 390 8.66e-18 85.1
Msa0659510 AT2G28040.2 25.130 386 230 17 45 404 32 384 1.52e-16 81.3
Msa0659510 AT2G28040.1 25.130 386 230 17 45 404 41 393 1.94e-16 80.9
Msa0659510 AT3G51340.1 26.121 379 213 16 72 401 99 459 2.15e-16 81.6
Msa0659510 AT3G51340.2 25.401 374 210 15 72 401 99 447 6.58e-16 80.1
Msa0659510 AT2G03200.1 23.798 416 222 20 45 404 85 461 1.55e-15 78.6
Msa0659510 AT1G66180.1 22.572 381 217 14 77 404 74 429 4.12e-15 77.0
Msa0659510 AT1G01300.1 27.005 374 199 17 73 401 140 484 2.44e-14 75.1
Msa0659510 AT4G35880.2 26.648 349 193 17 69 376 100 426 2.69e-14 74.7
Msa0659510 AT3G59080.2 24.359 390 239 15 44 404 137 499 2.69e-14 75.1
Msa0659510 AT3G51330.2 25.641 351 195 17 72 376 98 428 9.04e-14 73.2
Msa0659510 AT3G51360.7 22.164 379 219 16 72 402 2 352 2.69e-13 71.6
Msa0659510 AT3G51360.9 22.951 366 226 14 74 402 100 446 3.87e-13 71.2
Msa0659510 AT3G51360.1 22.951 366 226 14 74 402 90 436 4.83e-13 70.9
Msa0659510 AT3G18490.1 23.925 372 209 19 73 401 160 500 1.59e-12 69.3
Msa0659510 AT3G20015.1 24.865 370 209 16 73 401 129 470 3.43e-12 68.2
Msa0659510 AT2G42980.1 24.742 388 218 14 73 404 158 527 5.27e-12 67.8
Msa0659510 AT3G59080.1 23.389 419 243 15 44 404 137 535 7.68e-12 67.4
Msa0659510 AT3G51340.3 26.820 261 148 9 79 300 107 363 1.98e-11 65.9
Msa0659510 AT3G51340.4 26.523 279 157 11 79 316 107 378 2.12e-11 65.5

Find 147 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTGTGATCCCTATTTA+TGG 0.123424 4_3:-69918610 Msa0659510:CDS
AAACTGAAATCTCTGGATTT+TGG 0.144007 4_3:+69915055 None:intergenic
AGATAGGTAGGGTGGGATTA+AGG 0.217206 4_3:-69918783 Msa0659510:CDS
TTTCTAAATGCAGCTGAATA+AGG 0.250514 4_3:-69915798 Msa0659510:intron
GGATAAACGTTGCCATAAAT+AGG 0.257987 4_3:+69918598 None:intergenic
AACGAATCGATTGATGAAAT+TGG 0.264253 4_3:+69916639 None:intergenic
ATTTCATAGTCACATTGATT+AGG 0.289901 4_3:+69917450 None:intergenic
CACCCTTTAGATGGAATACT+TGG 0.291237 4_3:-69916807 Msa0659510:CDS
TTACCAAGCAATGATTTCTT+TGG 0.294065 4_3:-69916396 Msa0659510:intron
AGTGTCAACATCAAGAAAAT+AGG 0.297686 4_3:+69917943 None:intergenic
TTTCATAGTCACATTGATTA+GGG 0.312831 4_3:+69917451 None:intergenic
AGCATTGTGTAGAGTATATT+GGG 0.314014 4_3:-69914879 Msa0659510:three_prime_UTR
TCAACTTACCACCCTTTAGA+TGG 0.325308 4_3:-69916816 Msa0659510:CDS
CTAAATTCTCACCTCCAATT+AGG 0.327781 4_3:+69915419 None:intergenic
AAGCATTGTGTAGAGTATAT+TGG 0.329838 4_3:-69914880 Msa0659510:three_prime_UTR
TTGGGTGAACTGAACCTAAT+TGG 0.334057 4_3:-69915433 Msa0659510:CDS
TCTTTAAAAGTACCTAATTA+AGG 0.339236 4_3:-69915028 Msa0659510:three_prime_UTR
GTCATATGCATGCCTTAATT+AGG 0.358204 4_3:+69915016 None:intergenic
TTTAAATTAATGATTCTTGT+TGG 0.369438 4_3:+69918938 None:intergenic
TGAAAGAAATTGAAGATTAA+TGG 0.372589 4_3:+69918897 None:intergenic
TCTTCTTGTTCAGCTTGGTT+TGG 0.372917 4_3:-69918721 Msa0659510:CDS
GATAGGTAGGGTGGGATTAA+GGG 0.398450 4_3:-69918782 Msa0659510:CDS
AGATCATAAACTGAAATCTC+TGG 0.399104 4_3:+69915048 None:intergenic
AGTTCACCCAATCCTACTTC+AGG 0.414059 4_3:+69915445 None:intergenic
GGAGCAGGAAAGAAGTTTCC+AGG 0.414558 4_3:-69919256 None:intergenic
AAGTTGAAGGTGAAAATATC+TGG 0.417456 4_3:+69916832 None:intergenic
GACAACGAGAAGCAGTTGAT+TGG 0.418942 4_3:-69915106 Msa0659510:CDS
GTGCCATTCTAACTTTAAGT+TGG 0.419171 4_3:+69917352 None:intergenic
AGAAGAAGATGAAGATAGGT+AGG 0.420924 4_3:-69918795 Msa0659510:exon
GATAAACGTTGCCATAAATA+GGG 0.423110 4_3:+69918599 None:intergenic
ATGGCAACGTTTATCCTGTT+GGG 0.427167 4_3:-69918591 Msa0659510:intron
GTGTCAACATCAAGAAAATA+GGG 0.431013 4_3:+69917944 None:intergenic
ATAGGTAAAGTCTGGTCATC+AGG 0.433183 4_3:+69915746 None:intergenic
AACAAGAAGAAGATGAAGAT+AGG 0.439947 4_3:-69918799 Msa0659510:exon
TTAAATATTATCTTTGACAC+TGG 0.441413 4_3:-69916449 Msa0659510:CDS
ACTTGTCTTCTTGTTCAGCT+TGG 0.441843 4_3:-69918726 Msa0659510:CDS
GAAGATGAAGATAGGTAGGG+TGG 0.442669 4_3:-69918791 Msa0659510:exon
CTGAGAGTTGAAGTATGTAT+AGG 0.443665 4_3:+69916421 None:intergenic
GATATTATAAGATAAGCTTC+AGG 0.447328 4_3:+69915608 None:intergenic
GATATCACAGTTGAATAGTC+AGG 0.453876 4_3:-69916760 Msa0659510:CDS
AAGTTGAAAAGTTGTAGAAA+AGG 0.460593 4_3:+69919127 None:intergenic
TTGGTGAAACTAAGTGTTAG+TGG 0.461386 4_3:+69915665 None:intergenic
AGTCCAAGTATTCCATCTAA+AGG 0.461510 4_3:+69916804 None:intergenic
GGTGGGATTAAGGGTGATGA+TGG 0.463114 4_3:-69918773 Msa0659510:CDS
AACTTTAAGTTGGACTCCAT+TGG 0.463263 4_3:+69917362 None:intergenic
TATGGCAACGTTTATCCTGT+TGG 0.466319 4_3:-69918592 Msa0659510:CDS
GTTATTTGTTGCAAGAATAT+GGG 0.467337 4_3:-69915496 Msa0659510:intron
TCCTCTATGATCTATAGTAA+TGG 0.469339 4_3:+69918824 None:intergenic
TTAGATGGAATACTTGGACT+TGG 0.471121 4_3:-69916801 Msa0659510:CDS
TGTACTAGTTCATGAAGAAA+AGG 0.472388 4_3:+69918972 None:intergenic
GAACGGCCCTGAAGTAGGAT+TGG 0.472405 4_3:-69915452 Msa0659510:CDS
TGATTGATTAGATTATTTCC+TGG 0.472792 4_3:+69919238 None:intergenic
GGAATACTATTGCAATCTGC+AGG 0.473909 4_3:+69915076 None:intergenic
TAATATTACCTCGGAACATT+GGG 0.474180 4_3:+69917880 None:intergenic
GTCCAAGTATTCCATCTAAA+GGG 0.477058 4_3:+69916805 None:intergenic
ATTAATGCATATTATAGAGC+AGG 0.483123 4_3:-69914967 Msa0659510:three_prime_UTR
ATCGTATCACGTTAATTTGA+CGG 0.486448 4_3:-69914792 Msa0659510:three_prime_UTR
ACATCAAGAAAATAGGGCCT+TGG 0.492843 4_3:+69917950 None:intergenic
AATGAAATATCATACCCAAC+AGG 0.493100 4_3:+69918577 None:intergenic
GGAGGAAGGAAGACCGGGGT+TGG 0.493414 4_3:-69916476 Msa0659510:CDS
CTTAAAGTTAGAATGGCACT+TGG 0.502205 4_3:-69917348 Msa0659510:CDS
GCCAGCACATAGGTAAAGTC+TGG 0.502564 4_3:+69915738 None:intergenic
AAGATGAAGATAGGTAGGGT+GGG 0.502567 4_3:-69918790 Msa0659510:CDS
AACGGCCCTGAAGTAGGATT+GGG 0.503963 4_3:-69915451 Msa0659510:CDS
GTTCACCCAATCCTACTTCA+GGG 0.504181 4_3:+69915446 None:intergenic
ATGATTCTTCTCGAATGAGT+TGG 0.504399 4_3:-69916665 Msa0659510:CDS
TACCATCACTTTGTCTAGCA+TGG 0.505530 4_3:+69915132 None:intergenic
ACAGTTGAATAGTCAGGGCT+TGG 0.506595 4_3:-69916754 Msa0659510:CDS
ATTGGGGTAAGCTATTATGC+AGG 0.506850 4_3:-69914862 Msa0659510:three_prime_UTR
AGGTTTAACTCTTCCACCAT+TGG 0.508559 4_3:+69915646 None:intergenic
AGTCCAACTTAAAGTTAGAA+TGG 0.510476 4_3:-69917355 Msa0659510:CDS
TATACCTTGTGAGTAAGAAA+AGG 0.511564 4_3:-69919053 Msa0659510:five_prime_UTR
TCAAGAAAATAGGGCCTTGG+TGG 0.511612 4_3:+69917953 None:intergenic
TACCTTCTCAACTTCACCAA+TGG 0.512014 4_3:-69917378 Msa0659510:CDS
ACGAGAAGCAGTTGATTGGA+TGG 0.515480 4_3:-69915102 Msa0659510:CDS
CTATACATACTTCAACTCTC+AGG 0.516576 4_3:-69916420 Msa0659510:CDS
GTAATATTACCTCGGAACAT+TGG 0.516797 4_3:+69917879 None:intergenic
ACCTCGGAACATTGGGTACA+AGG 0.517734 4_3:+69917887 None:intergenic
AGAAATTGAAGATTAATGGT+GGG 0.517801 4_3:+69918901 None:intergenic
TGTTTAAGCTCGCGAGGAGG+GGG 0.519451 4_3:-69916711 Msa0659510:CDS
TGGGAGAGCGTGTGAATGAA+TGG 0.519940 4_3:+69919187 None:intergenic
ATGGAATACTTGGACTTGGA+AGG 0.520338 4_3:-69916797 Msa0659510:CDS
TTTGGTAATAAACGCAAAAG+TGG 0.521006 4_3:-69918703 Msa0659510:CDS
ATTCTGAACGGCCCTGAAGT+AGG 0.523129 4_3:-69915457 Msa0659510:CDS
TGGTGAATGAGCAAGTGTTA+AGG 0.528403 4_3:+69919207 None:intergenic
CTTGAAGACATGGCTTCACC+TGG 0.531998 4_3:+69918664 None:intergenic
TCTAAAGGGTGGTAAGTTGA+AGG 0.532635 4_3:+69916819 None:intergenic
TGGAATACTTGGACTTGGAA+GGG 0.533058 4_3:-69916796 Msa0659510:CDS
TTCACATGTGTAGTCATCGG+TGG 0.533364 4_3:+69917476 None:intergenic
GAAAATAGGGCCTTGGTGGT+TGG 0.533650 4_3:+69917957 None:intergenic
AAGAAATTGAAGATTAATGG+TGG 0.534067 4_3:+69918900 None:intergenic
GGGCTTGGTGCGAAATGTGA+TGG 0.536228 4_3:-69916739 Msa0659510:CDS
GAAATTGAAGATTAATGGTG+GGG 0.539765 4_3:+69918902 None:intergenic
CGAGAAGCAGTTGATTGGAT+GGG 0.543766 4_3:-69915101 Msa0659510:CDS
AGGGGAAAACATTACTCTGC+AGG 0.544182 4_3:-69916518 Msa0659510:CDS
AATATGGGAAATGTTTGTCT+AGG 0.546632 4_3:-69915481 Msa0659510:CDS
ACCAGACTTTACCTATGTGC+TGG 0.546884 4_3:-69915739 Msa0659510:CDS
CTCCATTGGTGAAGTTGAGA+AGG 0.547745 4_3:+69917376 None:intergenic
ATAAACTCAACATTAGAAAG+TGG 0.548243 4_3:+69919001 None:intergenic
GGAATACTTGGACTTGGAAG+GGG 0.550065 4_3:-69916795 Msa0659510:CDS
ACACTTAGTTTCACCAATGG+TGG 0.552466 4_3:-69915659 Msa0659510:CDS
ATAAATAGGGATCACAATTG+AGG 0.553125 4_3:+69918612 None:intergenic
TGATTCTTGTTGGAAAGAGT+TGG 0.556940 4_3:+69918948 None:intergenic
AGTCTCATGAACCATGCTGC+TGG 0.557344 4_3:-69918637 Msa0659510:CDS
TCAGGAGGGATCTCAAACTG+AGG 0.558793 4_3:+69915626 None:intergenic
GAAGAAGATGAAGATAGGTA+GGG 0.561089 4_3:-69918794 Msa0659510:exon
GTCTTCACATGTGTAGTCAT+CGG 0.562506 4_3:+69917473 None:intergenic
GCTGATCAATACTCAACCCT+TGG 0.562559 4_3:-69917420 Msa0659510:CDS
TGACCAAAGAAATCATTGCT+TGG 0.564667 4_3:+69916393 None:intergenic
ACTTCATTTATGCTTCTGAA+AGG 0.566258 4_3:+69915704 None:intergenic
ATATCACAGTTGAATAGTCA+GGG 0.567767 4_3:-69916759 Msa0659510:CDS
GAGACTTGGTCTTGAAGACA+TGG 0.568180 4_3:+69918654 None:intergenic
ATTGTTTAAGCTCGCGAGGA+GGG 0.569291 4_3:-69916713 Msa0659510:CDS
TCACATGTGTAGTCATCGGT+GGG 0.576365 4_3:+69917477 None:intergenic
TCCATTACTATAGATCATAG+AGG 0.586543 4_3:-69918825 Msa0659510:five_prime_UTR
TATAATGTTACTCTCAACAT+AGG 0.586798 4_3:-69917980 Msa0659510:CDS
GCAGCATGGTTCATGAGACT+TGG 0.589743 4_3:+69918640 None:intergenic
CATTGTTTAAGCTCGCGAGG+AGG 0.594028 4_3:-69916714 Msa0659510:CDS
GAGAAGCAGTTGATTGGATG+GGG 0.594642 4_3:-69915100 Msa0659510:CDS
GGCTTGGTGCGAAATGTGAT+GGG 0.595102 4_3:-69916738 Msa0659510:CDS
CATATTATAGAGCAGGGTGA+TGG 0.595503 4_3:-69914960 Msa0659510:three_prime_UTR
ATTCAACTGTGATATCAAGC+TGG 0.597014 4_3:+69916767 None:intergenic
TTAATGCATATTATAGAGCA+GGG 0.602995 4_3:-69914966 Msa0659510:three_prime_UTR
ACTTGGACTTGGAAGGGGAA+AGG 0.604303 4_3:-69916790 Msa0659510:CDS
TTGGGGTAAGCTATTATGCA+GGG 0.608982 4_3:-69914861 Msa0659510:three_prime_UTR
CTAACACTTAGTTTCACCAA+TGG 0.609380 4_3:-69915662 Msa0659510:CDS
ACTTTACCTATGTGCTGGCA+TGG 0.609706 4_3:-69915734 Msa0659510:CDS
TTGTTTAAGCTCGCGAGGAG+GGG 0.611048 4_3:-69916712 Msa0659510:CDS
ATCATTTAGAAGTACACCAA+GGG 0.613371 4_3:+69917404 None:intergenic
GGACATTGTTTAAGCTCGCG+AGG 0.614419 4_3:-69916717 Msa0659510:CDS
AATATTTAAACTTCCAACCC+CGG 0.615370 4_3:+69916463 None:intergenic
TTAAAGTTAGAATGGCACTT+GGG 0.620505 4_3:-69917347 Msa0659510:intron
ATTATAAGATAAGCTTCAGG+AGG 0.624826 4_3:+69915611 None:intergenic
ATCTTTGCAAGATTCATGTG+TGG 0.629596 4_3:-69919077 Msa0659510:five_prime_UTR
ATCCATGCTAGACAAAGTGA+TGG 0.638414 4_3:-69915134 Msa0659510:CDS
ACACTGGTAGCGAACTCACA+TGG 0.642611 4_3:-69917925 Msa0659510:CDS
GGTGAACTGAACCTAATTGG+AGG 0.647086 4_3:-69915430 Msa0659510:intron
CAAGTATTCCATCTAAAGGG+TGG 0.648915 4_3:+69916808 None:intergenic
GGGTACAAGGAGCATCACAT+TGG 0.654731 4_3:+69917900 None:intergenic
GTTTGTCTAGGCATTCTGAA+CGG 0.654989 4_3:-69915469 Msa0659510:CDS
CATCATTTAGAAGTACACCA+AGG 0.656080 4_3:+69917403 None:intergenic
CAATTGAGGAACCAGCAGCA+TGG 0.660713 4_3:+69918626 None:intergenic
AATTTGACGGCATTAGCACG+AGG 0.660806 4_3:-69914779 None:intergenic
TCCTTGTACCCAATGTTCCG+AGG 0.664829 4_3:-69917888 Msa0659510:intron
GCATTGTGTAGAGTATATTG+GGG 0.685659 4_3:-69914878 Msa0659510:three_prime_UTR
TCAACATAGGCCAACCACCA+AGG 0.689202 4_3:-69917967 Msa0659510:CDS
TTATAAGATAAGCTTCAGGA+GGG 0.705622 4_3:+69915612 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATAATAATAATAAAT+GGG + chr4_3:69914868-69914887 None:intergenic 0.0%
!! ATAATAATAATAATAATAAA+TGG + chr4_3:69914869-69914888 None:intergenic 0.0%
!!! TTTATTTTTACTATAATGTA+TGG + chr4_3:69915163-69915182 None:intergenic 10.0%
!! ATTAAAATTCAATTCATAAA+AGG + chr4_3:69915585-69915604 None:intergenic 10.0%
!!! TGAATTGAATTTTAATTTTT+TGG - chr4_3:69915589-69915608 Msa0659510:intron 10.0%
!!! AAATTGATATTCATTTTTAT+TGG - chr4_3:69915845-69915864 Msa0659510:intron 10.0%
!!! ATTTATATTATTTTCTAGAA+TGG + chr4_3:69917965-69917984 None:intergenic 10.0%
!!! ATTAATAATTTCTAGTATAA+AGG + chr4_3:69918183-69918202 None:intergenic 10.0%
!! TTATACTAGAAATTATTAAT+TGG - chr4_3:69918183-69918202 Msa0659510:intron 10.0%
!! TTTAAATTAATGATTCTTGT+TGG + chr4_3:69915098-69915117 None:intergenic 15.0%
!!! TTTCTTGTTTATCAATTATT+AGG - chr4_3:69915696-69915715 Msa0659510:CDS 15.0%
!! ATATTATATGCAATTTATGT+AGG + chr4_3:69916424-69916443 None:intergenic 15.0%
!! ATGATTTATATAAGTGAATT+AGG - chr4_3:69916851-69916870 Msa0659510:intron 15.0%
!! ATTAACATAAGATATAATCA+AGG - chr4_3:69917898-69917917 Msa0659510:CDS 15.0%
!!! AATATTTTGTGATTTCAAAA+TGG - chr4_3:69917990-69918009 Msa0659510:intron 15.0%
!! TAAGTATTTAATGATGAAAT+TGG + chr4_3:69918119-69918138 None:intergenic 15.0%
!! TTGTTAACTGTTATAATAAA+TGG - chr4_3:69918480-69918499 Msa0659510:intron 15.0%
!! TGAAAGAAATTGAAGATTAA+TGG + chr4_3:69915139-69915158 None:intergenic 20.0%
!! ATGATATTTCATTTCTTTCT+AGG - chr4_3:69915466-69915485 Msa0659510:CDS 20.0%
!!! GGTTTTCTTTTATTTAATTG+TGG + chr4_3:69915801-69915820 None:intergenic 20.0%
!!! TCAGACAAATGTATTTTTTT+GGG - chr4_3:69915868-69915887 Msa0659510:intron 20.0%
!! ATTAGAATATCAATTTCATG+AGG + chr4_3:69915998-69916017 None:intergenic 20.0%
!! TTCACTTATATAAATCATTC+TGG + chr4_3:69916849-69916868 None:intergenic 20.0%
!! AATAGATATGACATAAATTG+TGG + chr4_3:69917117-69917136 None:intergenic 20.0%
!! TTAAATATTATCTTTGACAC+TGG - chr4_3:69917584-69917603 Msa0659510:intron 20.0%
!!! GTATTTTTAGTTAACTAGTT+TGG - chr4_3:69917832-69917851 Msa0659510:intron 20.0%
!! GCATTTAGAAAATTAACAAA+AGG + chr4_3:69918226-69918245 None:intergenic 20.0%
!! ATATTATGTTATGTAAGTGT+TGG - chr4_3:69918855-69918874 Msa0659510:five_prime_UTR 20.0%
!!! TCTTTAAAAGTACCTAATTA+AGG - chr4_3:69919005-69919024 Msa0659510:five_prime_UTR 20.0%
!!! GTTTTATTACTTTATCTTCA+TGG - chr4_3:69919105-69919124 Msa0659510:five_prime_UTR 20.0%
! TGATTGATTAGATTATTTCC+TGG + chr4_3:69914798-69914817 None:intergenic 25.0%
! AAGTTGAAAAGTTGTAGAAA+AGG + chr4_3:69914909-69914928 None:intergenic 25.0%
! ATAAACTCAACATTAGAAAG+TGG + chr4_3:69915035-69915054 None:intergenic 25.0%
! AGAAATTGAAGATTAATGGT+GGG + chr4_3:69915135-69915154 None:intergenic 25.0%
! AAGAAATTGAAGATTAATGG+TGG + chr4_3:69915136-69915155 None:intergenic 25.0%
! AATTTCAAAGCAACAGAAAA+GGG + chr4_3:69915722-69915741 None:intergenic 25.0%
! AAATTTCAAAGCAACAGAAA+AGG + chr4_3:69915723-69915742 None:intergenic 25.0%
!!! GTCAGACAAATGTATTTTTT+TGG - chr4_3:69915867-69915886 Msa0659510:intron 25.0%
!!! TGTATTTTTTTGGGACATTT+CGG - chr4_3:69915877-69915896 Msa0659510:intron 25.0%
! TATAATGTTACTCTCAACAT+AGG - chr4_3:69916053-69916072 Msa0659510:intron 25.0%
!!! ATGTGCTGATTATAATTGAT+TGG - chr4_3:69916349-69916368 Msa0659510:intron 25.0%
! CAGCAAAAAAAAAATTACTC+TGG - chr4_3:69916379-69916398 Msa0659510:intron 25.0%
! CAAAAAAAAAATTACTCTGG+TGG - chr4_3:69916382-69916401 Msa0659510:intron 25.0%
!! TTTCATAGTCACATTGATTA+GGG + chr4_3:69916585-69916604 None:intergenic 25.0%
!! ATTTCATAGTCACATTGATT+AGG + chr4_3:69916586-69916605 None:intergenic 25.0%
!! TGATTACATTACTTCTAAGA+GGG + chr4_3:69917690-69917709 None:intergenic 25.0%
! TTGATTACATTACTTCTAAG+AGG + chr4_3:69917691-69917710 None:intergenic 25.0%
! GTAATGTAATCAAGTAGTTA+TGG - chr4_3:69917698-69917717 Msa0659510:intron 25.0%
! TCAAGTAGTTATGGTTATTA+TGG - chr4_3:69917707-69917726 Msa0659510:intron 25.0%
! ATTATGTGTATTACACAACA+GGG + chr4_3:69918032-69918051 None:intergenic 25.0%
! AATTATGTGTATTACACAAC+AGG + chr4_3:69918033-69918052 None:intergenic 25.0%
! ACTAGAAATTATTAATTGGC+AGG - chr4_3:69918187-69918206 Msa0659510:intron 25.0%
! GATATTATAAGATAAGCTTC+AGG + chr4_3:69918428-69918447 None:intergenic 25.0%
!! TGTTATTTGTTGCAAGAATA+TGG - chr4_3:69918536-69918555 Msa0659510:intron 25.0%
!! GTTATTTGTTGCAAGAATAT+GGG - chr4_3:69918537-69918556 Msa0659510:intron 25.0%
! ATTAATGCATATTATAGAGC+AGG - chr4_3:69919066-69919085 Msa0659510:five_prime_UTR 25.0%
! TTAATGCATATTATAGAGCA+GGG - chr4_3:69919067-69919086 Msa0659510:five_prime_UTR 25.0%
TATACCTTGTGAGTAAGAAA+AGG - chr4_3:69914980-69914999 Msa0659510:three_prime_UTR 30.0%
! TTATCCTTTTCTTACTCACA+AGG + chr4_3:69914987-69915006 None:intergenic 30.0%
TGTACTAGTTCATGAAGAAA+AGG + chr4_3:69915064-69915083 None:intergenic 30.0%
GAAATTGAAGATTAATGGTG+GGG + chr4_3:69915134-69915153 None:intergenic 30.0%
TCCATTACTATAGATCATAG+AGG - chr4_3:69915208-69915227 Msa0659510:intron 30.0%
TCCTCTATGATCTATAGTAA+TGG + chr4_3:69915212-69915231 None:intergenic 30.0%
AACAAGAAGAAGATGAAGAT+AGG - chr4_3:69915234-69915253 Msa0659510:intron 30.0%
! TTTGGTAATAAACGCAAAAG+TGG - chr4_3:69915330-69915349 Msa0659510:intron 30.0%
ATAAATAGGGATCACAATTG+AGG + chr4_3:69915424-69915443 None:intergenic 30.0%
TCAATTGTGATCCCTATTTA+TGG - chr4_3:69915423-69915442 Msa0659510:intron 30.0%
GATAAACGTTGCCATAAATA+GGG + chr4_3:69915437-69915456 None:intergenic 30.0%
AATGAAATATCATACCCAAC+AGG + chr4_3:69915459-69915478 None:intergenic 30.0%
CTGCAAAAGTTGAAATTTAC+GGG + chr4_3:69916029-69916048 None:intergenic 30.0%
CCTGCAAAAGTTGAAATTTA+CGG + chr4_3:69916030-69916049 None:intergenic 30.0%
GTGTCAACATCAAGAAAATA+GGG + chr4_3:69916092-69916111 None:intergenic 30.0%
AGTGTCAACATCAAGAAAAT+AGG + chr4_3:69916093-69916112 None:intergenic 30.0%
! TATTTTCTTGATGTTGACAC+TGG - chr4_3:69916092-69916111 Msa0659510:intron 30.0%
TAATATTACCTCGGAACATT+GGG + chr4_3:69916156-69916175 None:intergenic 30.0%
CAGTTAGTGTAATATTACCT+CGG + chr4_3:69916165-69916184 None:intergenic 30.0%
ATGTATCTATAGGAAAGGAT+GGG + chr4_3:69916265-69916284 None:intergenic 30.0%
TATGTATCTATAGGAAAGGA+TGG + chr4_3:69916266-69916285 None:intergenic 30.0%
! ATCATTTAGAAGTACACCAA+GGG + chr4_3:69916632-69916651 None:intergenic 30.0%
AGTCCAACTTAAAGTTAGAA+TGG - chr4_3:69916678-69916697 Msa0659510:CDS 30.0%
TTAAAGTTAGAATGGCACTT+GGG - chr4_3:69916686-69916705 Msa0659510:CDS 30.0%
ATATTTCAAGAGAACATGCA+AGG + chr4_3:69916724-69916743 None:intergenic 30.0%
TTTGTGGAATGGTAAAACTT+AGG + chr4_3:69917011-69917030 None:intergenic 30.0%
TCAAATAGTAGATCATGCAT+TGG + chr4_3:69917036-69917055 None:intergenic 30.0%
TCAAACATGTCATTGTTGAA+AGG + chr4_3:69917080-69917099 None:intergenic 30.0%
TTGATTCCAATATACAGATG+TGG - chr4_3:69917172-69917191 Msa0659510:intron 30.0%
TCATATCCACATCTGTATAT+TGG + chr4_3:69917181-69917200 None:intergenic 30.0%
AAGTTGAAGGTGAAAATATC+TGG + chr4_3:69917204-69917223 None:intergenic 30.0%
ATATCACAGTTGAATAGTCA+GGG - chr4_3:69917274-69917293 Msa0659510:intron 30.0%
AACGAATCGATTGATGAAAT+TGG + chr4_3:69917397-69917416 None:intergenic 30.0%
TGTCACTAGTATAATACATG+TGG + chr4_3:69917446-69917465 None:intergenic 30.0%
!! TCTTTTATGTTGTTTGTGTC+AGG - chr4_3:69917495-69917514 Msa0659510:intron 30.0%
!! CTTTTATGTTGTTTGTGTCA+GGG - chr4_3:69917496-69917515 Msa0659510:intron 30.0%
!! TTTTATGTTGTTTGTGTCAG+GGG - chr4_3:69917497-69917516 Msa0659510:intron 30.0%
AATATTTAAACTTCCAACCC+CGG + chr4_3:69917573-69917592 None:intergenic 30.0%
TTACCAAGCAATGATTTCTT+TGG - chr4_3:69917637-69917656 Msa0659510:intron 30.0%
! ATTACATTACTTCTAAGAGG+GGG + chr4_3:69917688-69917707 None:intergenic 30.0%
! GATTACATTACTTCTAAGAG+GGG + chr4_3:69917689-69917708 None:intergenic 30.0%
TTTCAGTTTCACTATATCCA+AGG - chr4_3:69917929-69917948 Msa0659510:CDS 30.0%
TTTCTAAATGCAGCTGAATA+AGG - chr4_3:69918235-69918254 Msa0659510:intron 30.0%
ACTTCATTTATGCTTCTGAA+AGG + chr4_3:69918332-69918351 None:intergenic 30.0%
TTATAAGATAAGCTTCAGGA+GGG + chr4_3:69918424-69918443 None:intergenic 30.0%
ATTATAAGATAAGCTTCAGG+AGG + chr4_3:69918425-69918444 None:intergenic 30.0%
AATATGGGAAATGTTTGTCT+AGG - chr4_3:69918552-69918571 Msa0659510:intron 30.0%
! TTAGCTACATTGTCGTATTT+TGG - chr4_3:69918633-69918652 Msa0659510:CDS 30.0%
! AAACTGAAATCTCTGGATTT+TGG + chr4_3:69918981-69919000 None:intergenic 30.0%
AGATCATAAACTGAAATCTC+TGG + chr4_3:69918988-69919007 None:intergenic 30.0%
! AAGCATTGTGTAGAGTATAT+TGG - chr4_3:69919153-69919172 Msa0659510:five_prime_UTR 30.0%
AGCATTGTGTAGAGTATATT+GGG - chr4_3:69919154-69919173 Msa0659510:five_prime_UTR 30.0%
! ATCGTATCACGTTAATTTGA+CGG - chr4_3:69919241-69919260 Msa0659510:five_prime_UTR 30.0%
ATCTTTGCAAGATTCATGTG+TGG - chr4_3:69914956-69914975 Msa0659510:three_prime_UTR 35.0%
TGATTCTTGTTGGAAAGAGT+TGG + chr4_3:69915088-69915107 None:intergenic 35.0%
AGAAGAAGATGAAGATAGGT+AGG - chr4_3:69915238-69915257 Msa0659510:intron 35.0%
GAAGAAGATGAAGATAGGTA+GGG - chr4_3:69915239-69915258 Msa0659510:intron 35.0%
! GGAAGAAACTCAATTTTACC+AGG - chr4_3:69915351-69915370 Msa0659510:intron 35.0%
GGATAAACGTTGCCATAAAT+AGG + chr4_3:69915438-69915457 None:intergenic 35.0%
TGAGTATCCAAATTCATACC+AGG - chr4_3:69915660-69915679 Msa0659510:CDS 35.0%
AGAAAGTAATATTCCAGTCC+TGG + chr4_3:69915681-69915700 None:intergenic 35.0%
AAAACCTCTTCTGACATTGA+TGG - chr4_3:69915815-69915834 Msa0659510:intron 35.0%
AAACCTCTTCTGACATTGAT+GGG - chr4_3:69915816-69915835 Msa0659510:intron 35.0%
TTAACCCCTTAAACTCAAAC+CGG - chr4_3:69915959-69915978 Msa0659510:intron 35.0%
!! TTCAACCGGTTTGAGTTTAA+GGG + chr4_3:69915967-69915986 None:intergenic 35.0%
CAAAAGTTGAAATTTACGGG+CGG + chr4_3:69916026-69916045 None:intergenic 35.0%
! CCGTAAATTTCAACTTTTGC+AGG - chr4_3:69916027-69916046 Msa0659510:intron 35.0%
GTAATATTACCTCGGAACAT+TGG + chr4_3:69916157-69916176 None:intergenic 35.0%
GCGTTATGTATCTATAGGAA+AGG + chr4_3:69916270-69916289 None:intergenic 35.0%
TCAACGCGTTATGTATCTAT+AGG + chr4_3:69916275-69916294 None:intergenic 35.0%
CAGGGTATAAAATCATTGCT+AGG + chr4_3:69916508-69916527 None:intergenic 35.0%
! CATCATTTAGAAGTACACCA+AGG + chr4_3:69916633-69916652 None:intergenic 35.0%
!! AACTTTAAGTTGGACTCCAT+TGG + chr4_3:69916674-69916693 None:intergenic 35.0%
! GTGCCATTCTAACTTTAAGT+TGG + chr4_3:69916684-69916703 None:intergenic 35.0%
CTTAAAGTTAGAATGGCACT+TGG - chr4_3:69916685-69916704 Msa0659510:CDS 35.0%
GACATAAATTGTGGTCATCA+TGG + chr4_3:69917108-69917127 None:intergenic 35.0%
GTCCAAGTATTCCATCTAAA+GGG + chr4_3:69917231-69917250 None:intergenic 35.0%
AGTCCAAGTATTCCATCTAA+AGG + chr4_3:69917232-69917251 None:intergenic 35.0%
TTAGATGGAATACTTGGACT+TGG - chr4_3:69917232-69917251 Msa0659510:intron 35.0%
ATTCAACTGTGATATCAAGC+TGG + chr4_3:69917269-69917288 None:intergenic 35.0%
GATATCACAGTTGAATAGTC+AGG - chr4_3:69917273-69917292 Msa0659510:intron 35.0%
ATGATTCTTCTCGAATGAGT+TGG - chr4_3:69917368-69917387 Msa0659510:CDS 35.0%
CTGAGAGTTGAAGTATGTAT+AGG + chr4_3:69917615-69917634 None:intergenic 35.0%
CTATACATACTTCAACTCTC+AGG - chr4_3:69917613-69917632 Msa0659510:intron 35.0%
TGACCAAAGAAATCATTGCT+TGG + chr4_3:69917643-69917662 None:intergenic 35.0%
GTGTTTCTCGTTAGTTAGTT+TGG - chr4_3:69917801-69917820 Msa0659510:intron 35.0%
TTGGTGAAACTAAGTGTTAG+TGG + chr4_3:69918371-69918390 None:intergenic 35.0%
CTAACACTTAGTTTCACCAA+TGG - chr4_3:69918371-69918390 Msa0659510:intron 35.0%
CTAAATTCTCACCTCCAATT+AGG + chr4_3:69918617-69918636 None:intergenic 35.0%
!! TGGTGCTACATAGATAGTAA+TGG - chr4_3:69918779-69918798 Msa0659510:CDS 35.0%
GTCATATGCATGCCTTAATT+AGG + chr4_3:69919020-69919039 None:intergenic 35.0%
GCATTGTGTAGAGTATATTG+GGG - chr4_3:69919155-69919174 Msa0659510:five_prime_UTR 35.0%
TGGTGAATGAGCAAGTGTTA+AGG + chr4_3:69914829-69914848 None:intergenic 40.0%
AAGATGAAGATAGGTAGGGT+GGG - chr4_3:69915243-69915262 Msa0659510:intron 40.0%
ACTTGTCTTCTTGTTCAGCT+TGG - chr4_3:69915307-69915326 Msa0659510:intron 40.0%
TCTTCTTGTTCAGCTTGGTT+TGG - chr4_3:69915312-69915331 Msa0659510:intron 40.0%
TATGGCAACGTTTATCCTGT+TGG - chr4_3:69915441-69915460 Msa0659510:CDS 40.0%
ATGGCAACGTTTATCCTGTT+GGG - chr4_3:69915442-69915461 Msa0659510:CDS 40.0%
ATCCAAATTCATACCAGGAC+TGG - chr4_3:69915665-69915684 Msa0659510:CDS 40.0%
TTCCAGTCCTGGTATGAATT+TGG + chr4_3:69915670-69915689 None:intergenic 40.0%
AACCTCTTCTGACATTGATG+GGG - chr4_3:69915817-69915836 Msa0659510:intron 40.0%
!! TCAACCGGTTTGAGTTTAAG+GGG + chr4_3:69915966-69915985 None:intergenic 40.0%
!! CTTCAACCGGTTTGAGTTTA+AGG + chr4_3:69915968-69915987 None:intergenic 40.0%
ACATCAAGAAAATAGGGCCT+TGG + chr4_3:69916086-69916105 None:intergenic 40.0%
GTCTTCACATGTGTAGTCAT+CGG + chr4_3:69916563-69916582 None:intergenic 40.0%
TACCTTCTCAACTTCACCAA+TGG - chr4_3:69916655-69916674 Msa0659510:CDS 40.0%
TGCTATTGTCTCTATGTGTG+AGG + chr4_3:69916983-69917002 None:intergenic 40.0%
! TCTAAAGGGTGGTAAGTTGA+AGG + chr4_3:69917217-69917236 None:intergenic 40.0%
TCAACTTACCACCCTTTAGA+TGG - chr4_3:69917217-69917236 Msa0659510:intron 40.0%
CAAGTATTCCATCTAAAGGG+TGG + chr4_3:69917228-69917247 None:intergenic 40.0%
CACCCTTTAGATGGAATACT+TGG - chr4_3:69917226-69917245 Msa0659510:intron 40.0%
ATGGAATACTTGGACTTGGA+AGG - chr4_3:69917236-69917255 Msa0659510:intron 40.0%
TGGAATACTTGGACTTGGAA+GGG - chr4_3:69917237-69917256 Msa0659510:intron 40.0%
!!! CTGAACTTGTTTTTGGAGGA+AGG - chr4_3:69917543-69917562 Msa0659510:intron 40.0%
AGAATGGACACTATAGTCCT+TGG + chr4_3:69917949-69917968 None:intergenic 40.0%
TCATTTGCTCAATCAACTCG+AGG - chr4_3:69918069-69918088 Msa0659510:intron 40.0%
!!! TCATCAGGTGCTGCTTTTAT+TGG + chr4_3:69918275-69918294 None:intergenic 40.0%
ATAGGTAAAGTCTGGTCATC+AGG + chr4_3:69918290-69918309 None:intergenic 40.0%
ACACTTAGTTTCACCAATGG+TGG - chr4_3:69918374-69918393 Msa0659510:intron 40.0%
AGGTTTAACTCTTCCACCAT+TGG + chr4_3:69918390-69918409 None:intergenic 40.0%
GTTTGTCTAGGCATTCTGAA+CGG - chr4_3:69918564-69918583 Msa0659510:intron 40.0%
TTGGGTGAACTGAACCTAAT+TGG - chr4_3:69918600-69918619 Msa0659510:CDS 40.0%
! ACACCTTCAGTTTGAAGTGT+TGG - chr4_3:69918759-69918778 Msa0659510:CDS 40.0%
ATCCATGCTAGACAAAGTGA+TGG - chr4_3:69918899-69918918 Msa0659510:five_prime_UTR 40.0%
TACCATCACTTTGTCTAGCA+TGG + chr4_3:69918904-69918923 None:intergenic 40.0%
GGAATACTATTGCAATCTGC+AGG + chr4_3:69918960-69918979 None:intergenic 40.0%
CATATTATAGAGCAGGGTGA+TGG - chr4_3:69919073-69919092 Msa0659510:five_prime_UTR 40.0%
ATTGGGGTAAGCTATTATGC+AGG - chr4_3:69919171-69919190 Msa0659510:five_prime_UTR 40.0%
TTGGGGTAAGCTATTATGCA+GGG - chr4_3:69919172-69919191 Msa0659510:five_prime_UTR 40.0%
GAAGATGAAGATAGGTAGGG+TGG - chr4_3:69915242-69915261 Msa0659510:intron 45.0%
AGATAGGTAGGGTGGGATTA+AGG - chr4_3:69915250-69915269 Msa0659510:intron 45.0%
GATAGGTAGGGTGGGATTAA+GGG - chr4_3:69915251-69915270 Msa0659510:intron 45.0%
!! GAGACTTGGTCTTGAAGACA+TGG + chr4_3:69915382-69915401 None:intergenic 45.0%
ACCTCTTCTGACATTGATGG+GGG - chr4_3:69915818-69915837 Msa0659510:intron 45.0%
ATGAGGTTCGCTACTTCAAC+CGG + chr4_3:69915981-69916000 None:intergenic 45.0%
TCAAGAAAATAGGGCCTTGG+TGG + chr4_3:69916083-69916102 None:intergenic 45.0%
TCATTGCTAGGCTTGTAGAG+TGG + chr4_3:69916496-69916515 None:intergenic 45.0%
GCACAAAGTGGATCTTTGCA+GGG + chr4_3:69916526-69916545 None:intergenic 45.0%
TCACATGTGTAGTCATCGGT+GGG + chr4_3:69916559-69916578 None:intergenic 45.0%
TTCACATGTGTAGTCATCGG+TGG + chr4_3:69916560-69916579 None:intergenic 45.0%
GCTGATCAATACTCAACCCT+TGG - chr4_3:69916613-69916632 Msa0659510:intron 45.0%
CTCCATTGGTGAAGTTGAGA+AGG + chr4_3:69916660-69916679 None:intergenic 45.0%
ATGCCTAGACGACTACACAA+TGG - chr4_3:69916919-69916938 Msa0659510:intron 45.0%
! ATGCATTGGCGTTTGTGGAA+TGG + chr4_3:69917022-69917041 None:intergenic 45.0%
! AGATCATGCATTGGCGTTTG+TGG + chr4_3:69917027-69917046 None:intergenic 45.0%
GGAATACTTGGACTTGGAAG+GGG - chr4_3:69917238-69917257 Msa0659510:intron 45.0%
ACAGTTGAATAGTCAGGGCT+TGG - chr4_3:69917279-69917298 Msa0659510:intron 45.0%
ATTGTTTAAGCTCGCGAGGA+GGG - chr4_3:69917320-69917339 Msa0659510:intron 45.0%
AGGGGAAAACATTACTCTGC+AGG - chr4_3:69917515-69917534 Msa0659510:intron 45.0%
!! GGACCAGCTGAACTTGTTTT+TGG - chr4_3:69917536-69917555 Msa0659510:intron 45.0%
CCTCCAAAAACAAGTTCAGC+TGG + chr4_3:69917542-69917561 None:intergenic 45.0%
!!! CCAGCTGAACTTGTTTTTGG+AGG - chr4_3:69917539-69917558 Msa0659510:intron 45.0%
!! GTTTTTGGAGGAAGGAAGAC+CGG - chr4_3:69917551-69917570 Msa0659510:intron 45.0%
!! TTTTTGGAGGAAGGAAGACC+GGG - chr4_3:69917552-69917571 Msa0659510:intron 45.0%
GCTGACGCTTAACACTTTCA+AGG + chr4_3:69917759-69917778 None:intergenic 45.0%
TGATGCGCCAAACTGACTAA+CGG + chr4_3:69917874-69917893 None:intergenic 45.0%
ACCAGACTTTACCTATGTGC+TGG - chr4_3:69918294-69918313 Msa0659510:intron 45.0%
! ACTTTACCTATGTGCTGGCA+TGG - chr4_3:69918299-69918318 Msa0659510:intron 45.0%
! CTTTTACCATGCCAGCACAT+AGG + chr4_3:69918308-69918327 None:intergenic 45.0%
GTTCACCCAATCCTACTTCA+GGG + chr4_3:69918590-69918609 None:intergenic 45.0%
AGTTCACCCAATCCTACTTC+AGG + chr4_3:69918591-69918610 None:intergenic 45.0%
GGTGAACTGAACCTAATTGG+AGG - chr4_3:69918603-69918622 Msa0659510:CDS 45.0%
! CTTCAAACTGAAGGTGTGTC+TGG + chr4_3:69918756-69918775 None:intergenic 45.0%
GCACCAACACTTCAAACTGA+AGG + chr4_3:69918765-69918784 None:intergenic 45.0%
GACAACGAGAAGCAGTTGAT+TGG - chr4_3:69918927-69918946 Msa0659510:five_prime_UTR 45.0%
ACGAGAAGCAGTTGATTGGA+TGG - chr4_3:69918931-69918950 Msa0659510:five_prime_UTR 45.0%
CGAGAAGCAGTTGATTGGAT+GGG - chr4_3:69918932-69918951 Msa0659510:five_prime_UTR 45.0%
GAGAAGCAGTTGATTGGATG+GGG - chr4_3:69918933-69918952 Msa0659510:five_prime_UTR 45.0%
! TGGGAGAGCGTGTGAATGAA+TGG + chr4_3:69914849-69914868 None:intergenic 50.0%
GGTGGGATTAAGGGTGATGA+TGG - chr4_3:69915260-69915279 Msa0659510:intron 50.0%
CTTGAAGACATGGCTTCACC+TGG + chr4_3:69915372-69915391 None:intergenic 50.0%
GCAGCATGGTTCATGAGACT+TGG + chr4_3:69915396-69915415 None:intergenic 50.0%
AGTCTCATGAACCATGCTGC+TGG - chr4_3:69915396-69915415 Msa0659510:intron 50.0%
CAATTGAGGAACCAGCAGCA+TGG + chr4_3:69915410-69915429 None:intergenic 50.0%
TCCCCCATCAATGTCAGAAG+AGG + chr4_3:69915822-69915841 None:intergenic 50.0%
CTTCTGACATTGATGGGGGA+AGG - chr4_3:69915822-69915841 Msa0659510:intron 50.0%
TCAACATAGGCCAACCACCA+AGG - chr4_3:69916066-69916085 Msa0659510:intron 50.0%
! GAAAATAGGGCCTTGGTGGT+TGG + chr4_3:69916079-69916098 None:intergenic 50.0%
! ACACTGGTAGCGAACTCACA+TGG - chr4_3:69916108-69916127 Msa0659510:intron 50.0%
!! GGGTACAAGGAGCATCACAT+TGG + chr4_3:69916136-69916155 None:intergenic 50.0%
TCCTTGTACCCAATGTTCCG+AGG - chr4_3:69916145-69916164 Msa0659510:intron 50.0%
ACCTCGGAACATTGGGTACA+AGG + chr4_3:69916149-69916168 None:intergenic 50.0%
CTAGGCTTGTAGAGTGGATG+GGG + chr4_3:69916490-69916509 None:intergenic 50.0%
GCTAGGCTTGTAGAGTGGAT+GGG + chr4_3:69916491-69916510 None:intergenic 50.0%
TGCTAGGCTTGTAGAGTGGA+TGG + chr4_3:69916492-69916511 None:intergenic 50.0%
CGCACAAAGTGGATCTTTGC+AGG + chr4_3:69916527-69916546 None:intergenic 50.0%
CGACCATTGTGTAGTCGTCT+AGG + chr4_3:69916925-69916944 None:intergenic 50.0%
ACTTGGACTTGGAAGGGGAA+AGG - chr4_3:69917243-69917262 Msa0659510:intron 50.0%
!! GGCTTGGTGCGAAATGTGAT+GGG - chr4_3:69917295-69917314 Msa0659510:intron 50.0%
GGACATTGTTTAAGCTCGCG+AGG - chr4_3:69917316-69917335 Msa0659510:intron 50.0%
CATTGTTTAAGCTCGCGAGG+AGG - chr4_3:69917319-69917338 Msa0659510:intron 50.0%
TTGTTTAAGCTCGCGAGGAG+GGG - chr4_3:69917321-69917340 Msa0659510:intron 50.0%
GGAGGGGGATACATCTTCTT+TGG - chr4_3:69917337-69917356 Msa0659510:intron 50.0%
!! TTTTGGAGGAAGGAAGACCG+GGG - chr4_3:69917553-69917572 Msa0659510:intron 50.0%
GTGCCTTCCGTTAGTCAGTT+TGG - chr4_3:69917864-69917883 Msa0659510:intron 50.0%
GCCAGCACATAGGTAAAGTC+TGG + chr4_3:69918298-69918317 None:intergenic 50.0%
TCAGGAGGGATCTCAAACTG+AGG + chr4_3:69918410-69918429 None:intergenic 50.0%
ATTCTGAACGGCCCTGAAGT+AGG - chr4_3:69918576-69918595 Msa0659510:intron 50.0%
AACGGCCCTGAAGTAGGATT+GGG - chr4_3:69918582-69918601 Msa0659510:intron 50.0%
!! GGGCTTGGTGCGAAATGTGA+TGG - chr4_3:69917294-69917313 Msa0659510:intron 55.0%
TGTTTAAGCTCGCGAGGAGG+GGG - chr4_3:69917322-69917341 Msa0659510:intron 55.0%
GCGCCAAACTGACTAACGGA+AGG + chr4_3:69917870-69917889 None:intergenic 55.0%
GAACGGCCCTGAAGTAGGAT+TGG - chr4_3:69918581-69918600 Msa0659510:intron 55.0%
TCTGGTGTGCGAGACATGTC+TGG + chr4_3:69918738-69918757 None:intergenic 55.0%
GGCTGCAAGGACGCACAAAG+TGG + chr4_3:69916538-69916557 None:intergenic 60.0%
!! GTAGTCATCGGTGGGCTGCA+AGG + chr4_3:69916551-69916570 None:intergenic 60.0%
GGAGGAAGGAAGACCGGGGT+TGG - chr4_3:69917557-69917576 Msa0659510:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 69914782 69919273 69914782 ID=Msa0659510;Name=Msa0659510
chr4_3 mRNA 69914782 69919273 69914782 ID=Msa0659510-mRNA-1;Parent=Msa0659510;Name=Msa0659510-mRNA-1;_AED=0.04;_eAED=0.04;_QI=464|0.85|0.87|1|1|1|8|269|412
chr4_3 exon 69914782 69915160 69914782 ID=Msa0659510-mRNA-1:exon:20644;Parent=Msa0659510-mRNA-1
chr4_3 exon 69915431 69915503 69915431 ID=Msa0659510-mRNA-1:exon:20643;Parent=Msa0659510-mRNA-1
chr4_3 exon 69915607 69915807 69915607 ID=Msa0659510-mRNA-1:exon:20642;Parent=Msa0659510-mRNA-1
chr4_3 exon 69916397 69916538 69916397 ID=Msa0659510-mRNA-1:exon:20641;Parent=Msa0659510-mRNA-1
chr4_3 exon 69916642 69916866 69916642 ID=Msa0659510-mRNA-1:exon:20640;Parent=Msa0659510-mRNA-1
chr4_3 exon 69917348 69917499 69917348 ID=Msa0659510-mRNA-1:exon:20639;Parent=Msa0659510-mRNA-1
chr4_3 exon 69917889 69918006 69917889 ID=Msa0659510-mRNA-1:exon:20638;Parent=Msa0659510-mRNA-1
chr4_3 exon 69918592 69919273 69918592 ID=Msa0659510-mRNA-1:exon:20637;Parent=Msa0659510-mRNA-1
chr4_3 five_prime_UTR 69918810 69919273 69918810 ID=Msa0659510-mRNA-1:five_prime_utr;Parent=Msa0659510-mRNA-1
chr4_3 CDS 69918592 69918809 69918592 ID=Msa0659510-mRNA-1:cds;Parent=Msa0659510-mRNA-1
chr4_3 CDS 69917889 69918006 69917889 ID=Msa0659510-mRNA-1:cds;Parent=Msa0659510-mRNA-1
chr4_3 CDS 69917348 69917499 69917348 ID=Msa0659510-mRNA-1:cds;Parent=Msa0659510-mRNA-1
chr4_3 CDS 69916642 69916866 69916642 ID=Msa0659510-mRNA-1:cds;Parent=Msa0659510-mRNA-1
chr4_3 CDS 69916397 69916538 69916397 ID=Msa0659510-mRNA-1:cds;Parent=Msa0659510-mRNA-1
chr4_3 CDS 69915607 69915807 69915607 ID=Msa0659510-mRNA-1:cds;Parent=Msa0659510-mRNA-1
chr4_3 CDS 69915431 69915503 69915431 ID=Msa0659510-mRNA-1:cds;Parent=Msa0659510-mRNA-1
chr4_3 CDS 69915051 69915160 69915051 ID=Msa0659510-mRNA-1:cds;Parent=Msa0659510-mRNA-1
chr4_3 three_prime_UTR 69914782 69915050 69914782 ID=Msa0659510-mRNA-1:three_prime_utr;Parent=Msa0659510-mRNA-1
Gene Sequence

>Msa0659510

ATGAAGATAGGTAGGGTGGGATTAAGGGTGATGATGGTTGCATTGATGATGATGATGTTAAACTTGTCTTCTTGTTCAGCTTGGTTTGGTAATAAACGCAAAAGTGGAAGAAACTCAATTTTACCAGGTGAAGCCATGTCTTCAAGACCAAGTCTCATGAACCATGCTGCTGGTTCCTCAATTGTGATCCCTATTTATGGCAACGTTTATCCTGTTGGGTTCTATAATGTTACTCTCAACATAGGCCAACCACCAAGGCCCTATTTTCTTGATGTTGACACTGGTAGCGAACTCACATGGCTCCAATGTGATGCTCCTTGTACCCAATGTTCCGAGCCCACCGATGACTACACATGTGAAGACCCTAATCAATGTGACTATGAAATTAAGTATGCTGATCAATACTCAACCCTTGGTGTACTTCTAAATGATGTTTACCTTCTCAACTTCACCAATGGAGTCCAACTTAAAGTTAGAATGGCACTTGGATGTGGATATGACCAGATATTTTCACCTTCAACTTACCACCCTTTAGATGGAATACTTGGACTTGGAAGGGGAAAGGCCAGCTTGATATCACAGTTGAATAGTCAGGGCTTGGTGCGAAATGTGATGGGACATTGTTTAAGCTCGCGAGGAGGGGGATACATCTTCTTTGGAAATGCTTATGATTCTTCTCGAATGAGTTGGACTCCAATTTCATCAATCGATTCGGGAAAACATTACTCTGCAGGACCAGCTGAACTTGTTTTTGGAGGAAGGAAGACCGGGGTTGGAAGTTTAAATATTATCTTTGACACTGGAAGTTCCTATACATACTTCAACTCTCAGGCTTACCAAGCAATGATTTCTTTGCTGAATAAGGAATTACATAGAAAGCCAATAAAAGCAGCACCTGATGACCAGACTTTACCTATGTGCTGGCATGGTAAAAGACCTTTCAGAAGCATAAATGAAGTGAAGAAATACTTCAAGCCACTAACACTTAGTTTCACCAATGGTGGAAGAGTTAAACCTCAGTTTGAGATCCCTCCTGAAGCTTATCTTATAATATCAAATATGGGAAATGTTTGTCTAGGCATTCTGAACGGCCCTGAAGTAGGATTGGGTGAACTGAACCTAATTGGAGACATATCCATGCTAGACAAAGTGATGGTATTCGACAACGAGAAGCAGTTGATTGGATGGGGACCTGCAGATTGCAATAGTATTCCAAAATCCAGAGATTTCAGTTTATGA
Protein sequence

>Msa0659510

MKIGRVGLRVMMVALMMMMLNLSSCSAWFGNKRKSGRNSILPGEAMSSRPSLMNHAAGSSIVIPIYGNVYPVGFYNVTLNIGQPPRPYFLDVDTGSELTWLQCDAPCTQCSEPTDDYTCEDPNQCDYEIKYADQYSTLGVLLNDVYLLNFTNGVQLKVRMALGCGYDQIFSPSTYHPLDGILGLGRGKASLISQLNSQGLVRNVMGHCLSSRGGGYIFFGNAYDSSRMSWTPISSIDSGKHYSAGPAELVFGGRKTGVGSLNIIFDTGSSYTYFNSQAYQAMISLLNKELHRKPIKAAPDDQTLPMCWHGKRPFRSINEVKKYFKPLTLSFTNGGRVKPQFEIPPEAYLIISNMGNVCLGILNGPEVGLGELNLIGDISMLDKVMVFDNEKQLIGWGPADCNSIPKSRDFSL*