AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0099260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099260 MtrunA17_Chr1g0151071 95.276 254 4 3 3 254 1 248 2.23e-172 474
Msa0099260 MtrunA17_Chr3g0136361 52.841 176 68 4 1 175 1 162 3.45e-54 174
Msa0099260 MtrunA17_Chr4g0070451 41.916 167 78 7 77 233 66 223 2.57e-23 94.7
Msa0099260 MtrunA17_Chr0c27g0493891 41.060 151 70 7 93 233 3 144 4.72e-17 76.3
Msa0099260 MtrunA17_Chr1g0168551 41.860 86 44 3 52 137 48 127 4.50e-14 68.6
Msa0099260 MtrunA17_Chr1g0147921 43.023 86 44 2 52 137 52 132 5.12e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099260 AT5G21940.1 54.015 137 50 6 46 176 44 173 9.12e-33 120
Msa0099260 AT3G43850.2 49.606 127 38 5 46 172 30 130 6.85e-27 103
Msa0099260 AT3G43850.1 50.000 142 43 6 33 172 15 130 1.59e-26 104

Find 53 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTGTATATTTATAATAA+TGG 0.261116 1_3:-5365481 None:intergenic
TGTATAGTTGATGATATATA+CGG 0.285224 1_3:+5367504 Msa0099260:three_prime_UTR
TAGAAGAGGTTCTTCCTATT+AGG 0.287448 1_3:+5365800 Msa0099260:CDS
GCCACTTCCACCGCGTCATC+CGG 0.302841 1_3:+5367193 Msa0099260:CDS
ATAAGGAATGGTTACCTAAT+AGG 0.349508 1_3:-5365814 None:intergenic
CCAACTATTATTTGTTGGTT+CGG 0.374739 1_3:-5367561 None:intergenic
CTTCTTGTGTATGCATTCTC+TGG 0.380516 1_3:-5366960 None:intergenic
TCAAACCAACTATTATTTGT+TGG 0.400637 1_3:-5367566 None:intergenic
AATTTGCTGGCTTTCAATCA+TGG 0.423862 1_3:+5366990 Msa0099260:CDS
AAGAAGACGTAGAAATTTGC+TGG 0.426140 1_3:+5366977 Msa0099260:CDS
GCTGGCTTTCAATCATGGTT+GGG 0.432602 1_3:+5366995 Msa0099260:CDS
AGTATCTCAAACTTCTATAG+TGG 0.435354 1_3:+5366870 Msa0099260:CDS
AAAGAAGACACTCTATTTCC+CGG 0.439102 1_3:-5367212 None:intergenic
CCGAACCAACAAATAATAGT+TGG 0.439930 1_3:+5367561 Msa0099260:three_prime_UTR
CGCGGTGGAAGTGGCGAAGA+AGG 0.445270 1_3:-5367185 None:intergenic
GAAGCTGAGAGTAAATATAA+TGG 0.445348 1_3:+5365751 Msa0099260:CDS
TGCTGGCTTTCAATCATGGT+TGG 0.445425 1_3:+5366994 Msa0099260:CDS
TTCCCTTTGAGAAGCAATAG+TGG 0.449201 1_3:+5367032 Msa0099260:CDS
AAACCGATGGAAAGATGGTT+TGG 0.451187 1_3:+5367610 Msa0099260:three_prime_UTR
CCCGGATGACGCGGTGGAAG+TGG 0.453467 1_3:-5367194 None:intergenic
AGCTAGGCTTGTGAAGGACT+TGG 0.454561 1_3:-5366898 None:intergenic
TTGGAAAACCGATGGAAAGA+TGG 0.469621 1_3:+5367605 Msa0099260:three_prime_UTR
CACCACTATTGCTTCTCAAA+GGG 0.472099 1_3:-5367034 None:intergenic
CTGTGTTATTTGACGCGCCG+AGG 0.485567 1_3:+5365599 Msa0099260:CDS
CCACCACTATTGCTTCTCAA+AGG 0.509782 1_3:-5367035 None:intergenic
TGTGTTATTTGACGCGCCGA+GGG 0.520967 1_3:+5365600 Msa0099260:CDS
CCACTTCCACCGCGTCATCC+GGG 0.531769 1_3:+5367194 Msa0099260:CDS
AGAAGGTGCTGAAGAATATG+AGG 0.542161 1_3:-5367168 None:intergenic
GAGAGATCAATGGATGAGAA+TGG 0.545446 1_3:+5365721 Msa0099260:CDS
GAAAAGAAATTCCTCTGCAA+AGG 0.546535 1_3:-5367242 None:intergenic
TGGTGGTGCTTTAGATTGCA+TGG 0.548478 1_3:+5365771 Msa0099260:CDS
GTATATTTATAATAATGGAA+AGG 0.553135 1_3:-5365476 None:intergenic
GATGATTATGGAGCTTGAAA+CGG 0.556496 1_3:+5365546 Msa0099260:exon
AACGGTGGCGATTAAGCGCG+TGG 0.559763 1_3:+5365564 Msa0099260:CDS
ATACCAAACCATCTTTCCAT+CGG 0.566229 1_3:-5367613 None:intergenic
CTTTAGCATCTCCTTTGCAG+AGG 0.570257 1_3:+5367231 Msa0099260:CDS
GTATAGTTGATGATATATAC+GGG 0.584796 1_3:+5367505 Msa0099260:three_prime_UTR
GGTTTCATCAGAGAGATCAA+TGG 0.590893 1_3:+5365711 Msa0099260:CDS
TCATCATCGTCATGATCCCT+CGG 0.599874 1_3:-5365616 None:intergenic
CCTTTGAGAAGCAATAGTGG+TGG 0.600401 1_3:+5367035 Msa0099260:CDS
TTGCATGGAAGCTTTAGAAG+AGG 0.606872 1_3:+5365786 Msa0099260:CDS
AGTTGATGATATATACGGGT+CGG 0.610096 1_3:+5367509 Msa0099260:three_prime_UTR
CTATTTCCCGGATGACGCGG+TGG 0.613883 1_3:-5367200 None:intergenic
ACAATGATGCAGATCAGCCA+AGG 0.617708 1_3:-5367264 None:intergenic
AACATCAGCTAGGCTTGTGA+AGG 0.619105 1_3:-5366904 None:intergenic
ACGACGTCGTCTTCATCGAT+CGG 0.620053 1_3:+5365664 Msa0099260:CDS
GTGTTGTGACAACATCAGCT+AGG 0.640480 1_3:-5366914 None:intergenic
AAATAGTGATGATGATGATG+AGG 0.659114 1_3:+5365690 Msa0099260:CDS
ACTCTATTTCCCGGATGACG+CGG 0.665825 1_3:-5367203 None:intergenic
GCTGAGAGTAAATATAATGG+TGG 0.695812 1_3:+5365754 Msa0099260:CDS
TGATGATGATGATCAAGGGA+AGG 0.697875 1_3:+5365633 Msa0099260:CDS
GATTATGGAGCTTGAAACGG+TGG 0.699571 1_3:+5365549 Msa0099260:CDS
ACGGTGGCGATTAAGCGCGT+GGG 0.700701 1_3:+5365565 Msa0099260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTGTATATTTATAATAA+TGG - chr1_3:5365484-5365503 None:intergenic 10.0%
!!! AAAGATTGTATATTTTTTTT+TGG + chr1_3:5366069-5366088 Msa0099260:intron 10.0%
!!! AAGATTGTATATTTTTTTTT+GGG + chr1_3:5366070-5366089 Msa0099260:intron 10.0%
!! ATTTATATCAAAATTTACTA+TGG + chr1_3:5366742-5366761 Msa0099260:intron 10.0%
!! TGTTTGTATATTTATAATAA+TGG - chr1_3:5365484-5365503 None:intergenic 10.0%
!!! AAAGATTGTATATTTTTTTT+TGG + chr1_3:5366069-5366088 Msa0099260:intron 10.0%
!!! AAGATTGTATATTTTTTTTT+GGG + chr1_3:5366070-5366089 Msa0099260:intron 10.0%
!! ATTTATATCAAAATTTACTA+TGG + chr1_3:5366742-5366761 Msa0099260:intron 10.0%
!!! GTATATTTATAATAATGGAA+AGG - chr1_3:5365479-5365498 None:intergenic 15.0%
!!! TTTTTTTAAATGTTTTAAGC+AGG + chr1_3:5366033-5366052 Msa0099260:intron 15.0%
!!! TTTTTTAAATGTTTTAAGCA+GGG + chr1_3:5366034-5366053 Msa0099260:intron 15.0%
!! GGTATTAGTTAAATTAATTA+GGG + chr1_3:5366141-5366160 Msa0099260:intron 15.0%
!!! AATATTGCCTTTTTTATTTT+TGG + chr1_3:5366536-5366555 Msa0099260:intron 15.0%
!!! GTATATTTATAATAATGGAA+AGG - chr1_3:5365479-5365498 None:intergenic 15.0%
!!! TTTTTTTAAATGTTTTAAGC+AGG + chr1_3:5366033-5366052 Msa0099260:intron 15.0%
!!! TTTTTTAAATGTTTTAAGCA+GGG + chr1_3:5366034-5366053 Msa0099260:intron 15.0%
!! GGTATTAGTTAAATTAATTA+GGG + chr1_3:5366141-5366160 Msa0099260:intron 15.0%
!!! AATATTGCCTTTTTTATTTT+TGG + chr1_3:5366536-5366555 Msa0099260:intron 15.0%
!!! TCTTTTCATTTTGATGATTA+TGG + chr1_3:5365534-5365553 Msa0099260:exon 20.0%
!! TAATGGAAAAACTGTTAATA+AGG - chr1_3:5365834-5365853 None:intergenic 20.0%
!! GGGTATTAGTTAAATTAATT+AGG + chr1_3:5366140-5366159 Msa0099260:intron 20.0%
!! AAAAGTTTGTGAATGAAATT+TGG + chr1_3:5366193-5366212 Msa0099260:intron 20.0%
!!! AATTTTGCTAAAAATAGCAA+GGG + chr1_3:5366343-5366362 Msa0099260:intron 20.0%
!! TGATATAAATCTACACAATT+TGG - chr1_3:5366732-5366751 None:intergenic 20.0%
!!! ATTAGTGTTCTAAATAATGA+AGG + chr1_3:5366816-5366835 Msa0099260:intron 20.0%
!!! TCATTTTCAATTGAAGAATT+TGG - chr1_3:5367314-5367333 None:intergenic 20.0%
!!! GTTGTATTTTAGTTATCATA+AGG - chr1_3:5367368-5367387 None:intergenic 20.0%
!!! TGTATAGTTGATGATATATA+CGG + chr1_3:5367504-5367523 Msa0099260:three_prime_UTR 20.0%
!!! TCTTTTCATTTTGATGATTA+TGG + chr1_3:5365534-5365553 Msa0099260:exon 20.0%
!! TAATGGAAAAACTGTTAATA+AGG - chr1_3:5365834-5365853 None:intergenic 20.0%
!! GGGTATTAGTTAAATTAATT+AGG + chr1_3:5366140-5366159 Msa0099260:intron 20.0%
!! AAAAGTTTGTGAATGAAATT+TGG + chr1_3:5366193-5366212 Msa0099260:intron 20.0%
!!! AATTTTGCTAAAAATAGCAA+GGG + chr1_3:5366343-5366362 Msa0099260:intron 20.0%
!! TGATATAAATCTACACAATT+TGG - chr1_3:5366732-5366751 None:intergenic 20.0%
!!! ATTAGTGTTCTAAATAATGA+AGG + chr1_3:5366816-5366835 Msa0099260:intron 20.0%
!!! TCATTTTCAATTGAAGAATT+TGG - chr1_3:5367314-5367333 None:intergenic 20.0%
!!! GTTGTATTTTAGTTATCATA+AGG - chr1_3:5367368-5367387 None:intergenic 20.0%
!!! TGTATAGTTGATGATATATA+CGG + chr1_3:5367504-5367523 Msa0099260:three_prime_UTR 20.0%
! GAAAAACTGTTAATAAGGAA+TGG - chr1_3:5365829-5365848 None:intergenic 25.0%
! AACAAAACATTCAAAAGTGA+AGG - chr1_3:5365919-5365938 None:intergenic 25.0%
!! GGTATTAGTTAAATTAGCTT+GGG + chr1_3:5366091-5366110 Msa0099260:intron 25.0%
!! AATTGTTGGATCAATTTTTG+TGG + chr1_3:5366118-5366137 Msa0099260:intron 25.0%
!! ATTGTTGGATCAATTTTTGT+GGG + chr1_3:5366119-5366138 Msa0099260:intron 25.0%
!! ATTAATTAGGGTACTATTGT+TGG + chr1_3:5366153-5366172 Msa0099260:intron 25.0%
!! AATTTGGTGATTGTATTGTA+AGG + chr1_3:5366209-5366228 Msa0099260:intron 25.0%
!! TTTTGTGAAACTGAAACAAA+GGG - chr1_3:5366299-5366318 None:intergenic 25.0%
!! ATTTTGTGAAACTGAAACAA+AGG - chr1_3:5366300-5366319 None:intergenic 25.0%
! TGTTTCAGTTTCACAAAATT+AGG + chr1_3:5366301-5366320 Msa0099260:intron 25.0%
!!! ATTTTTAGCAAAATTCCACA+TGG - chr1_3:5366338-5366357 None:intergenic 25.0%
!! GAATTTTGCTAAAAATAGCA+AGG + chr1_3:5366342-5366361 Msa0099260:intron 25.0%
!! CTTTTGATAGAAGAAAACTT+GGG + chr1_3:5366442-5366461 Msa0099260:intron 25.0%
! ATCCAATTCAAAAGTTTATC+CGG - chr1_3:5366501-5366520 None:intergenic 25.0%
! TCCTCAACCAAAAATAAAAA+AGG - chr1_3:5366546-5366565 None:intergenic 25.0%
!! AAGGCTAATCCAATATTTTA+TGG + chr1_3:5366835-5366854 Msa0099260:intron 25.0%
!! TTTACAATGTCTTTTACAGA+TGG - chr1_3:5366939-5366958 None:intergenic 25.0%
!!! TAAGGTTTTATACACTATGA+TGG - chr1_3:5367350-5367369 None:intergenic 25.0%
!!! GTATAGTTGATGATATATAC+GGG + chr1_3:5367505-5367524 Msa0099260:three_prime_UTR 25.0%
! TCAAACCAACTATTATTTGT+TGG - chr1_3:5367569-5367588 None:intergenic 25.0%
!! TGATCTTACAATCTTTTCTT+TGG + chr1_3:5367586-5367605 Msa0099260:three_prime_UTR 25.0%
! GAAAAACTGTTAATAAGGAA+TGG - chr1_3:5365829-5365848 None:intergenic 25.0%
! AACAAAACATTCAAAAGTGA+AGG - chr1_3:5365919-5365938 None:intergenic 25.0%
!! GGTATTAGTTAAATTAGCTT+GGG + chr1_3:5366091-5366110 Msa0099260:intron 25.0%
!! AATTGTTGGATCAATTTTTG+TGG + chr1_3:5366118-5366137 Msa0099260:intron 25.0%
!! ATTGTTGGATCAATTTTTGT+GGG + chr1_3:5366119-5366138 Msa0099260:intron 25.0%
!! ATTAATTAGGGTACTATTGT+TGG + chr1_3:5366153-5366172 Msa0099260:intron 25.0%
!! AATTTGGTGATTGTATTGTA+AGG + chr1_3:5366209-5366228 Msa0099260:intron 25.0%
!! TTTTGTGAAACTGAAACAAA+GGG - chr1_3:5366299-5366318 None:intergenic 25.0%
!! ATTTTGTGAAACTGAAACAA+AGG - chr1_3:5366300-5366319 None:intergenic 25.0%
! TGTTTCAGTTTCACAAAATT+AGG + chr1_3:5366301-5366320 Msa0099260:intron 25.0%
!!! ATTTTTAGCAAAATTCCACA+TGG - chr1_3:5366338-5366357 None:intergenic 25.0%
!! GAATTTTGCTAAAAATAGCA+AGG + chr1_3:5366342-5366361 Msa0099260:intron 25.0%
!! CTTTTGATAGAAGAAAACTT+GGG + chr1_3:5366442-5366461 Msa0099260:intron 25.0%
! ATCCAATTCAAAAGTTTATC+CGG - chr1_3:5366501-5366520 None:intergenic 25.0%
! TCCTCAACCAAAAATAAAAA+AGG - chr1_3:5366546-5366565 None:intergenic 25.0%
!! AAGGCTAATCCAATATTTTA+TGG + chr1_3:5366835-5366854 Msa0099260:intron 25.0%
!! TTTACAATGTCTTTTACAGA+TGG - chr1_3:5366939-5366958 None:intergenic 25.0%
!!! TAAGGTTTTATACACTATGA+TGG - chr1_3:5367350-5367369 None:intergenic 25.0%
!!! GTATAGTTGATGATATATAC+GGG + chr1_3:5367505-5367524 Msa0099260:three_prime_UTR 25.0%
! TCAAACCAACTATTATTTGT+TGG - chr1_3:5367569-5367588 None:intergenic 25.0%
!! TGATCTTACAATCTTTTCTT+TGG + chr1_3:5367586-5367605 Msa0099260:three_prime_UTR 25.0%
AGACACGAAAATGCAAAAAA+TGG - chr1_3:5365510-5365529 None:intergenic 30.0%
! AAATAGTGATGATGATGATG+AGG + chr1_3:5365690-5365709 Msa0099260:CDS 30.0%
GAAGCTGAGAGTAAATATAA+TGG + chr1_3:5365751-5365770 Msa0099260:CDS 30.0%
ATAAGGAATGGTTACCTAAT+AGG - chr1_3:5365817-5365836 None:intergenic 30.0%
GGGTATTAGTTAAATTAGCT+TGG + chr1_3:5366090-5366109 Msa0099260:intron 30.0%
! TTGTTGGATCAATTTTTGTG+GGG + chr1_3:5366120-5366139 Msa0099260:intron 30.0%
!!! TAGGTTTATTTTGTACCATG+TGG + chr1_3:5366320-5366339 Msa0099260:intron 30.0%
! GCTTTTGATAGAAGAAAACT+TGG + chr1_3:5366441-5366460 Msa0099260:intron 30.0%
CTAAAGAACATGTTTGCAAT+TGG + chr1_3:5366471-5366490 Msa0099260:intron 30.0%
! TACCGGATAAACTTTTGAAT+TGG + chr1_3:5366496-5366515 Msa0099260:intron 30.0%
!!! GCCTTTTTTATTTTTGGTTG+AGG + chr1_3:5366542-5366561 Msa0099260:intron 30.0%
! TAATTCATGTTTGCCTTGTT+TGG + chr1_3:5366574-5366593 Msa0099260:intron 30.0%
GTTTGAATCAACTTTCATTC+TGG - chr1_3:5366703-5366722 None:intergenic 30.0%
CCTGCATAACCATAAAATAT+TGG - chr1_3:5366847-5366866 None:intergenic 30.0%
! CCAATATTTTATGGTTATGC+AGG + chr1_3:5366844-5366863 Msa0099260:intron 30.0%
AGTATCTCAAACTTCTATAG+TGG + chr1_3:5366870-5366889 Msa0099260:CDS 30.0%
! CCAACTATTATTTGTTGGTT+CGG - chr1_3:5367564-5367583 None:intergenic 30.0%
AGACACGAAAATGCAAAAAA+TGG - chr1_3:5365510-5365529 None:intergenic 30.0%
! AAATAGTGATGATGATGATG+AGG + chr1_3:5365690-5365709 Msa0099260:CDS 30.0%
GAAGCTGAGAGTAAATATAA+TGG + chr1_3:5365751-5365770 Msa0099260:CDS 30.0%
ATAAGGAATGGTTACCTAAT+AGG - chr1_3:5365817-5365836 None:intergenic 30.0%
GGGTATTAGTTAAATTAGCT+TGG + chr1_3:5366090-5366109 Msa0099260:intron 30.0%
! TTGTTGGATCAATTTTTGTG+GGG + chr1_3:5366120-5366139 Msa0099260:intron 30.0%
!!! TAGGTTTATTTTGTACCATG+TGG + chr1_3:5366320-5366339 Msa0099260:intron 30.0%
! GCTTTTGATAGAAGAAAACT+TGG + chr1_3:5366441-5366460 Msa0099260:intron 30.0%
CTAAAGAACATGTTTGCAAT+TGG + chr1_3:5366471-5366490 Msa0099260:intron 30.0%
! TACCGGATAAACTTTTGAAT+TGG + chr1_3:5366496-5366515 Msa0099260:intron 30.0%
!!! GCCTTTTTTATTTTTGGTTG+AGG + chr1_3:5366542-5366561 Msa0099260:intron 30.0%
! TAATTCATGTTTGCCTTGTT+TGG + chr1_3:5366574-5366593 Msa0099260:intron 30.0%
GTTTGAATCAACTTTCATTC+TGG - chr1_3:5366703-5366722 None:intergenic 30.0%
CCTGCATAACCATAAAATAT+TGG - chr1_3:5366847-5366866 None:intergenic 30.0%
! CCAATATTTTATGGTTATGC+AGG + chr1_3:5366844-5366863 Msa0099260:intron 30.0%
AGTATCTCAAACTTCTATAG+TGG + chr1_3:5366870-5366889 Msa0099260:CDS 30.0%
! CCAACTATTATTTGTTGGTT+CGG - chr1_3:5367564-5367583 None:intergenic 30.0%
GATGATTATGGAGCTTGAAA+CGG + chr1_3:5365546-5365565 Msa0099260:exon 35.0%
! ACGATGATGATGATGATCAA+GGG + chr1_3:5365629-5365648 Msa0099260:CDS 35.0%
GCTGAGAGTAAATATAATGG+TGG + chr1_3:5365754-5365773 Msa0099260:CDS 35.0%
TAGAAGAGGTTCTTCCTATT+AGG + chr1_3:5365800-5365819 Msa0099260:CDS 35.0%
! TTAGCTTGGGTACTAATTGT+TGG + chr1_3:5366104-5366123 Msa0099260:intron 35.0%
! TGTGTGATGATGTGATCTAT+AGG + chr1_3:5366253-5366272 Msa0099260:intron 35.0%
CCAAAACATGACGAATTCAT+TGG - chr1_3:5366395-5366414 None:intergenic 35.0%
! CCAATGAATTCGTCATGTTT+TGG + chr1_3:5366392-5366411 Msa0099260:intron 35.0%
GCATGATTGAAGCTTACAAA+AGG - chr1_3:5366423-5366442 None:intergenic 35.0%
TTGCAAACAAGTACCAAACA+AGG - chr1_3:5366590-5366609 None:intergenic 35.0%
TGTTTGGTACTTGTTTGCAA+TGG + chr1_3:5366590-5366609 Msa0099260:intron 35.0%
GCAACGTGATTGTATAGTTT+CGG + chr1_3:5366638-5366657 Msa0099260:intron 35.0%
CAACGTGATTGTATAGTTTC+GGG + chr1_3:5366639-5366658 Msa0099260:intron 35.0%
AAGAAGACGTAGAAATTTGC+TGG + chr1_3:5366977-5366996 Msa0099260:CDS 35.0%
!! AATTTGCTGGCTTTCAATCA+TGG + chr1_3:5366990-5367009 Msa0099260:CDS 35.0%
AAAGAAGACACTCTATTTCC+CGG - chr1_3:5367215-5367234 None:intergenic 35.0%
GAAAAGAAATTCCTCTGCAA+AGG - chr1_3:5367245-5367264 None:intergenic 35.0%
! CATTTTCATTGTTGCAGCAA+TGG - chr1_3:5367291-5367310 None:intergenic 35.0%
! ATTTTCTTTCAGCGAGAAAG+AGG + chr1_3:5367426-5367445 Msa0099260:three_prime_UTR 35.0%
!! AGTTGATGATATATACGGGT+CGG + chr1_3:5367509-5367528 Msa0099260:three_prime_UTR 35.0%
CCGAACCAACAAATAATAGT+TGG + chr1_3:5367561-5367580 Msa0099260:three_prime_UTR 35.0%
!! TCTTTTCTTTGGAAAACCGA+TGG + chr1_3:5367597-5367616 Msa0099260:three_prime_UTR 35.0%
GATGATTATGGAGCTTGAAA+CGG + chr1_3:5365546-5365565 Msa0099260:exon 35.0%
! ACGATGATGATGATGATCAA+GGG + chr1_3:5365629-5365648 Msa0099260:CDS 35.0%
GCTGAGAGTAAATATAATGG+TGG + chr1_3:5365754-5365773 Msa0099260:CDS 35.0%
TAGAAGAGGTTCTTCCTATT+AGG + chr1_3:5365800-5365819 Msa0099260:CDS 35.0%
! TTAGCTTGGGTACTAATTGT+TGG + chr1_3:5366104-5366123 Msa0099260:intron 35.0%
! TGTGTGATGATGTGATCTAT+AGG + chr1_3:5366253-5366272 Msa0099260:intron 35.0%
CCAAAACATGACGAATTCAT+TGG - chr1_3:5366395-5366414 None:intergenic 35.0%
! CCAATGAATTCGTCATGTTT+TGG + chr1_3:5366392-5366411 Msa0099260:intron 35.0%
GCATGATTGAAGCTTACAAA+AGG - chr1_3:5366423-5366442 None:intergenic 35.0%
TTGCAAACAAGTACCAAACA+AGG - chr1_3:5366590-5366609 None:intergenic 35.0%
TGTTTGGTACTTGTTTGCAA+TGG + chr1_3:5366590-5366609 Msa0099260:intron 35.0%
GCAACGTGATTGTATAGTTT+CGG + chr1_3:5366638-5366657 Msa0099260:intron 35.0%
CAACGTGATTGTATAGTTTC+GGG + chr1_3:5366639-5366658 Msa0099260:intron 35.0%
AAGAAGACGTAGAAATTTGC+TGG + chr1_3:5366977-5366996 Msa0099260:CDS 35.0%
!! AATTTGCTGGCTTTCAATCA+TGG + chr1_3:5366990-5367009 Msa0099260:CDS 35.0%
AAAGAAGACACTCTATTTCC+CGG - chr1_3:5367215-5367234 None:intergenic 35.0%
GAAAAGAAATTCCTCTGCAA+AGG - chr1_3:5367245-5367264 None:intergenic 35.0%
! CATTTTCATTGTTGCAGCAA+TGG - chr1_3:5367291-5367310 None:intergenic 35.0%
! ATTTTCTTTCAGCGAGAAAG+AGG + chr1_3:5367426-5367445 Msa0099260:three_prime_UTR 35.0%
!! AGTTGATGATATATACGGGT+CGG + chr1_3:5367509-5367528 Msa0099260:three_prime_UTR 35.0%
CCGAACCAACAAATAATAGT+TGG + chr1_3:5367561-5367580 Msa0099260:three_prime_UTR 35.0%
!! TCTTTTCTTTGGAAAACCGA+TGG + chr1_3:5367597-5367616 Msa0099260:three_prime_UTR 35.0%
! GACGATGATGATGATGATCA+AGG + chr1_3:5365628-5365647 Msa0099260:CDS 40.0%
! TGATGATGATGATCAAGGGA+AGG + chr1_3:5365633-5365652 Msa0099260:CDS 40.0%
GGTTTCATCAGAGAGATCAA+TGG + chr1_3:5365711-5365730 Msa0099260:CDS 40.0%
GAGAGATCAATGGATGAGAA+TGG + chr1_3:5365721-5365740 Msa0099260:CDS 40.0%
!! TTGCATGGAAGCTTTAGAAG+AGG + chr1_3:5365786-5365805 Msa0099260:CDS 40.0%
ATCACATCATCACACACTAC+TGG - chr1_3:5366249-5366268 None:intergenic 40.0%
CATGTTTGCAATTGGTGTAC+CGG + chr1_3:5366479-5366498 Msa0099260:intron 40.0%
CTGTGAGTTTGACGATATCA+TGG + chr1_3:5366613-5366632 Msa0099260:intron 40.0%
TGAGTTTGACGATATCATGG+TGG + chr1_3:5366616-5366635 Msa0099260:intron 40.0%
CTTCTTGTGTATGCATTCTC+TGG - chr1_3:5366963-5366982 None:intergenic 40.0%
! TTCCCTTTGAGAAGCAATAG+TGG + chr1_3:5367032-5367051 Msa0099260:CDS 40.0%
CACCACTATTGCTTCTCAAA+GGG - chr1_3:5367037-5367056 None:intergenic 40.0%
!! AGAAGGTGCTGAAGAATATG+AGG - chr1_3:5367171-5367190 None:intergenic 40.0%
! GCAGAGGAATTTCTTTTCCT+TGG + chr1_3:5367247-5367266 Msa0099260:CDS 40.0%
TTGGAAAACCGATGGAAAGA+TGG + chr1_3:5367605-5367624 Msa0099260:three_prime_UTR 40.0%
AAACCGATGGAAAGATGGTT+TGG + chr1_3:5367610-5367629 Msa0099260:three_prime_UTR 40.0%
! GACGATGATGATGATGATCA+AGG + chr1_3:5365628-5365647 Msa0099260:CDS 40.0%
! TGATGATGATGATCAAGGGA+AGG + chr1_3:5365633-5365652 Msa0099260:CDS 40.0%
GGTTTCATCAGAGAGATCAA+TGG + chr1_3:5365711-5365730 Msa0099260:CDS 40.0%
GAGAGATCAATGGATGAGAA+TGG + chr1_3:5365721-5365740 Msa0099260:CDS 40.0%
!! TTGCATGGAAGCTTTAGAAG+AGG + chr1_3:5365786-5365805 Msa0099260:CDS 40.0%
ATCACATCATCACACACTAC+TGG - chr1_3:5366249-5366268 None:intergenic 40.0%
CATGTTTGCAATTGGTGTAC+CGG + chr1_3:5366479-5366498 Msa0099260:intron 40.0%
CTGTGAGTTTGACGATATCA+TGG + chr1_3:5366613-5366632 Msa0099260:intron 40.0%
TGAGTTTGACGATATCATGG+TGG + chr1_3:5366616-5366635 Msa0099260:intron 40.0%
CTTCTTGTGTATGCATTCTC+TGG - chr1_3:5366963-5366982 None:intergenic 40.0%
! TTCCCTTTGAGAAGCAATAG+TGG + chr1_3:5367032-5367051 Msa0099260:CDS 40.0%
CACCACTATTGCTTCTCAAA+GGG - chr1_3:5367037-5367056 None:intergenic 40.0%
!! AGAAGGTGCTGAAGAATATG+AGG - chr1_3:5367171-5367190 None:intergenic 40.0%
! GCAGAGGAATTTCTTTTCCT+TGG + chr1_3:5367247-5367266 Msa0099260:CDS 40.0%
TTGGAAAACCGATGGAAAGA+TGG + chr1_3:5367605-5367624 Msa0099260:three_prime_UTR 40.0%
AAACCGATGGAAAGATGGTT+TGG + chr1_3:5367610-5367629 Msa0099260:three_prime_UTR 40.0%
GATTATGGAGCTTGAAACGG+TGG + chr1_3:5365549-5365568 Msa0099260:CDS 45.0%
TCATCATCGTCATGATCCCT+CGG - chr1_3:5365619-5365638 None:intergenic 45.0%
! TGGTGGTGCTTTAGATTGCA+TGG + chr1_3:5365771-5365790 Msa0099260:CDS 45.0%
AACATCAGCTAGGCTTGTGA+AGG - chr1_3:5366907-5366926 None:intergenic 45.0%
GTGTTGTGACAACATCAGCT+AGG - chr1_3:5366917-5366936 None:intergenic 45.0%
!! TGCTGGCTTTCAATCATGGT+TGG + chr1_3:5366994-5367013 Msa0099260:CDS 45.0%
!! GCTGGCTTTCAATCATGGTT+GGG + chr1_3:5366995-5367014 Msa0099260:CDS 45.0%
CCACCACTATTGCTTCTCAA+AGG - chr1_3:5367038-5367057 None:intergenic 45.0%
! CCTTTGAGAAGCAATAGTGG+TGG + chr1_3:5367035-5367054 Msa0099260:CDS 45.0%
CTTTAGCATCTCCTTTGCAG+AGG + chr1_3:5367231-5367250 Msa0099260:CDS 45.0%
ACAATGATGCAGATCAGCCA+AGG - chr1_3:5367267-5367286 None:intergenic 45.0%
GATTATGGAGCTTGAAACGG+TGG + chr1_3:5365549-5365568 Msa0099260:CDS 45.0%
TCATCATCGTCATGATCCCT+CGG - chr1_3:5365619-5365638 None:intergenic 45.0%
! TGGTGGTGCTTTAGATTGCA+TGG + chr1_3:5365771-5365790 Msa0099260:CDS 45.0%
AACATCAGCTAGGCTTGTGA+AGG - chr1_3:5366907-5366926 None:intergenic 45.0%
GTGTTGTGACAACATCAGCT+AGG - chr1_3:5366917-5366936 None:intergenic 45.0%
!! TGCTGGCTTTCAATCATGGT+TGG + chr1_3:5366994-5367013 Msa0099260:CDS 45.0%
!! GCTGGCTTTCAATCATGGTT+GGG + chr1_3:5366995-5367014 Msa0099260:CDS 45.0%
CCACCACTATTGCTTCTCAA+AGG - chr1_3:5367038-5367057 None:intergenic 45.0%
! CCTTTGAGAAGCAATAGTGG+TGG + chr1_3:5367035-5367054 Msa0099260:CDS 45.0%
CTTTAGCATCTCCTTTGCAG+AGG + chr1_3:5367231-5367250 Msa0099260:CDS 45.0%
ACAATGATGCAGATCAGCCA+AGG - chr1_3:5367267-5367286 None:intergenic 45.0%
!! ATATATATACATATATATAA+TGG - chr1_3:5365851-5365870 None:intergenic 5.0%
!! ATATATATACATATATATAA+TGG - chr1_3:5365851-5365870 None:intergenic 5.0%
!! TGTGTTATTTGACGCGCCGA+GGG + chr1_3:5365600-5365619 Msa0099260:CDS 50.0%
ACGACGTCGTCTTCATCGAT+CGG + chr1_3:5365664-5365683 Msa0099260:CDS 50.0%
AGCTAGGCTTGTGAAGGACT+TGG - chr1_3:5366901-5366920 None:intergenic 50.0%
ACTCTATTTCCCGGATGACG+CGG - chr1_3:5367206-5367225 None:intergenic 50.0%
!! TGTGTTATTTGACGCGCCGA+GGG + chr1_3:5365600-5365619 Msa0099260:CDS 50.0%
ACGACGTCGTCTTCATCGAT+CGG + chr1_3:5365664-5365683 Msa0099260:CDS 50.0%
AGCTAGGCTTGTGAAGGACT+TGG - chr1_3:5366901-5366920 None:intergenic 50.0%
ACTCTATTTCCCGGATGACG+CGG - chr1_3:5367206-5367225 None:intergenic 50.0%
!! CTGTGTTATTTGACGCGCCG+AGG + chr1_3:5365599-5365618 Msa0099260:CDS 55.0%
!! CTGTGTTATTTGACGCGCCG+AGG + chr1_3:5365599-5365618 Msa0099260:CDS 55.0%
AACGGTGGCGATTAAGCGCG+TGG + chr1_3:5365564-5365583 Msa0099260:CDS 60.0%
ACGGTGGCGATTAAGCGCGT+GGG + chr1_3:5365565-5365584 Msa0099260:CDS 60.0%
CTATTTCCCGGATGACGCGG+TGG - chr1_3:5367203-5367222 None:intergenic 60.0%
AACGGTGGCGATTAAGCGCG+TGG + chr1_3:5365564-5365583 Msa0099260:CDS 60.0%
ACGGTGGCGATTAAGCGCGT+GGG + chr1_3:5365565-5365584 Msa0099260:CDS 60.0%
CTATTTCCCGGATGACGCGG+TGG - chr1_3:5367203-5367222 None:intergenic 60.0%
CGCGGTGGAAGTGGCGAAGA+AGG - chr1_3:5367188-5367207 None:intergenic 65.0%
GCCACTTCCACCGCGTCATC+CGG + chr1_3:5367193-5367212 Msa0099260:CDS 65.0%
CCACTTCCACCGCGTCATCC+GGG + chr1_3:5367194-5367213 Msa0099260:CDS 65.0%
CGCGGTGGAAGTGGCGAAGA+AGG - chr1_3:5367188-5367207 None:intergenic 65.0%
GCCACTTCCACCGCGTCATC+CGG + chr1_3:5367193-5367212 Msa0099260:CDS 65.0%
CCACTTCCACCGCGTCATCC+GGG + chr1_3:5367194-5367213 Msa0099260:CDS 65.0%
CCCGGATGACGCGGTGGAAG+TGG - chr1_3:5367197-5367216 None:intergenic 70.0%
CCCGGATGACGCGGTGGAAG+TGG - chr1_3:5367197-5367216 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 5365459 5367633 5365459 ID=Msa0099260;Name=Msa0099260
chr1_3 mRNA 5365459 5367633 5365459 ID=Msa0099260-mRNA-1;Parent=Msa0099260;Name=Msa0099260-mRNA-1;_AED=0.21;_eAED=0.21;_QI=88|1|1|1|1|1|2|278|254
chr1_3 exon 5365459 5365821 5365459 ID=Msa0099260-mRNA-1:exon:1951;Parent=Msa0099260-mRNA-1
chr1_3 exon 5366866 5367633 5366866 ID=Msa0099260-mRNA-1:exon:1952;Parent=Msa0099260-mRNA-1
chr1_3 five_prime_UTR 5365459 5365546 5365459 ID=Msa0099260-mRNA-1:five_prime_utr;Parent=Msa0099260-mRNA-1
chr1_3 CDS 5365547 5365821 5365547 ID=Msa0099260-mRNA-1:cds;Parent=Msa0099260-mRNA-1
chr1_3 CDS 5366866 5367355 5366866 ID=Msa0099260-mRNA-1:cds;Parent=Msa0099260-mRNA-1
chr1_3 three_prime_UTR 5367356 5367633 5367356 ID=Msa0099260-mRNA-1:three_prime_utr;Parent=Msa0099260-mRNA-1
Gene Sequence

>Msa0099260

ATGATTATGGAGCTTGAAACGGTGGCGATTAAGCGCGTGGGTTCACGTACTTCTGTGTTATTTGACGCGCCGAGGGATCATGACGATGATGATGATGATCAAGGGAAGGTGTGTAGTACGACGTCGTCTTCATCGATCGGAAGAAATAGTGATGATGATGATGAGGTTTCATCAGAGAGATCAATGGATGAGAATGGTGATAATGAAGCTGAGAGTAAATATAATGGTGGTGCTTTAGATTGCATGGAAGCTTTAGAAGAGGTTCTTCCTATTAGGAGAAGTATCTCAAACTTCTATAGTGGAAAATCCAAGTCCTTCACAAGCCTAGCTGATGTTGTCACAACACCATCTGTAAAAGACATTGTAAAACCAGAGAATGCATACACAAGAAGACGTAGAAATTTGCTGGCTTTCAATCATGGTTGGGACAAGAACAAAAATTTCCCTTTGAGAAGCAATAGTGGTGGAATTTCAAAGAGAACAATGAGCTTAAGTAGAAGTGCTTTAGCTCTTGCAGTTGCATTAAGCAATTCTGATAGTAGCTCTAGTTTTACAAGTGATGATTCAGCTGCTTCAACCTCATATTCTTCAGCACCTTCTTCGCCACTTCCACCGCGTCATCCGGGAAATAGAGTGTCTTCTTTAGCATCTCCTTTGCAGAGGAATTTCTTTTCCTTGGCTGATCTGCATCATTGTGCCATTGCTGCAACAATGAAAATGCCAAATTCTTCAATTGAAAATGAAACAACTAATCATCCATCATAG
Protein sequence

>Msa0099260

MIMELETVAIKRVGSRTSVLFDAPRDHDDDDDDQGKVCSTTSSSSIGRNSDDDDEVSSERSMDENGDNEAESKYNGGALDCMEALEEVLPIRRSISNFYSGKSKSFTSLADVVTTPSVKDIVKPENAYTRRRRNLLAFNHGWDKNKNFPLRSNSGGISKRTMSLSRSALALAVALSNSDSSSSFTSDDSAASTSYSSAPSSPLPPRHPGNRVSSLASPLQRNFFSLADLHHCAIAATMKMPNSSIENETTNHPS*