AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0148040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148040 MtrunA17_Chr1g0151071 94.882 254 4 3 3 253 1 248 1.05e-170 471
Msa0148040 MtrunA17_Chr3g0136361 53.143 175 68 4 1 174 1 162 8.68e-54 173
Msa0148040 MtrunA17_Chr4g0070451 41.916 167 78 7 76 232 66 223 2.18e-23 95.1
Msa0148040 MtrunA17_Chr0c27g0493891 41.060 151 70 7 92 232 3 144 5.44e-17 76.3
Msa0148040 MtrunA17_Chr1g0168551 43.023 86 43 3 51 136 48 127 1.38e-14 70.1
Msa0148040 MtrunA17_Chr1g0147921 41.860 86 45 2 51 136 52 132 5.43e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148040 AT5G21940.1 55.147 136 49 5 46 175 44 173 6.92e-35 126
Msa0148040 AT3G43850.2 50.794 126 37 5 46 171 30 130 2.38e-27 104
Msa0148040 AT3G43850.1 50.794 126 37 5 46 171 30 130 5.46e-27 105

Find 52 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAATAATGGTTACCTTAT+AGG 0.166825 1_4:-8224771 None:intergenic
TAGAAGAGGTTCTTCCTATA+AGG 0.279952 1_4:+8224757 Msa0148040:CDS
TGTATAGTTGATGATATATA+CGG 0.285224 1_4:+8226437 Msa0148040:three_prime_UTR
GCCACTTCCACCGCGTCATC+CGG 0.302841 1_4:+8226128 Msa0148040:CDS
CCAACTATTATTTGTTGGTT+CGG 0.374739 1_4:-8226494 None:intergenic
CTTCTTGTGTATGCATTCTC+TGG 0.380516 1_4:-8225895 None:intergenic
TCAAACCAACTATTATTTGT+TGG 0.400637 1_4:-8226499 None:intergenic
TGGTGGTGCTTTAGATTGTA+TGG 0.406572 1_4:+8224728 Msa0148040:CDS
AATTTGCTGGCTTTCAATCA+TGG 0.423862 1_4:+8225925 Msa0148040:CDS
AAGAAGACGTAGAAATTTGC+TGG 0.426140 1_4:+8225912 Msa0148040:CDS
GCTGGCTTTCAATCATGGTT+GGG 0.432602 1_4:+8225930 Msa0148040:CDS
AAAGAAGACACTCTATTTCC+CGG 0.439102 1_4:-8226147 None:intergenic
CCGAACCAACAAATAATAGT+TGG 0.439930 1_4:+8226494 Msa0148040:three_prime_UTR
CGCGGTGGAAGTGGCGAAGA+AGG 0.445270 1_4:-8226120 None:intergenic
GAAGCTGAGAGTAAATATAA+TGG 0.445348 1_4:+8224708 Msa0148040:CDS
TGCTGGCTTTCAATCATGGT+TGG 0.445425 1_4:+8225929 Msa0148040:CDS
TTCCCTTTGAGAAGCAATAG+TGG 0.449201 1_4:+8225967 Msa0148040:CDS
AAACCGATGGAAAGATGGTT+TGG 0.451187 1_4:+8226543 Msa0148040:three_prime_UTR
CCCGGATGACGCGGTGGAAG+TGG 0.453467 1_4:-8226129 None:intergenic
CTGTATTATTTGACGCGCGA+AGG 0.467561 1_4:+8224559 Msa0148040:CDS
TTGGAAAACCGATGGAAAGA+TGG 0.469621 1_4:+8226538 Msa0148040:three_prime_UTR
CACCACTATTGCTTCTCAAA+GGG 0.472099 1_4:-8225969 None:intergenic
TGTATTATTTGACGCGCGAA+GGG 0.506332 1_4:+8224560 Msa0148040:CDS
CCACCACTATTGCTTCTCAA+AGG 0.509782 1_4:-8225970 None:intergenic
GTATCTCAAATTTCTATAGC+GGG 0.512706 1_4:+8225806 Msa0148040:CDS
CCACTTCCACCGCGTCATCC+GGG 0.531769 1_4:+8226129 Msa0148040:CDS
GAGAGATCAATGGACGAGAA+TGG 0.534280 1_4:+8224678 Msa0148040:CDS
AACGGTGGCGTTTAAGCGCG+TGG 0.535037 1_4:+8224524 Msa0148040:CDS
AGAAGGTGCTGAAGAATATG+AGG 0.542161 1_4:-8226103 None:intergenic
GAAAAGAAATTCCTCTGCAA+AGG 0.546535 1_4:-8226177 None:intergenic
GATGATTATGGAGCTTGAAA+CGG 0.556496 1_4:+8224506 Msa0148040:exon
AACATCAGCTAGGCTTGTAA+AGG 0.557041 1_4:-8225839 None:intergenic
ATACCAAACCATCTTTCCAT+CGG 0.566229 1_4:-8226546 None:intergenic
CTTTAGCATCTCCTTTGCAG+AGG 0.570257 1_4:+8226166 Msa0148040:CDS
AAGAAATAGTGATGATGATG+AGG 0.574683 1_4:+8224647 Msa0148040:CDS
TTGTATGGAGGCTTTAGAAG+AGG 0.575224 1_4:+8224743 Msa0148040:CDS
GTATAGTTGATGATATATAC+GGG 0.584796 1_4:+8226438 Msa0148040:three_prime_UTR
GGTTTCATCAGAGAGATCAA+TGG 0.590893 1_4:+8224668 Msa0148040:CDS
ATGGATTTATAATAATGGAA+AGG 0.591403 1_4:-8224449 None:intergenic
AGTATCTCAAATTTCTATAG+CGG 0.596091 1_4:+8225805 Msa0148040:CDS
CCTTTGAGAAGCAATAGTGG+TGG 0.600401 1_4:+8225970 Msa0148040:CDS
AGTTGATGATATATACGGGT+CGG 0.610096 1_4:+8226442 Msa0148040:three_prime_UTR
CTATTTCCCGGATGACGCGG+TGG 0.613883 1_4:-8226135 None:intergenic
ACAATGATGCAGATCAGCCA+AGG 0.617708 1_4:-8226199 None:intergenic
ACGACGTCGTCTTCATCGAT+CGG 0.620053 1_4:+8224624 Msa0148040:CDS
GTGTTGTGACAACATCAGCT+AGG 0.640480 1_4:-8225849 None:intergenic
ACGGTGGCGTTTAAGCGCGT+GGG 0.660985 1_4:+8224525 Msa0148040:CDS
TGGTGCTTTAGATTGTATGG+AGG 0.662476 1_4:+8224731 Msa0148040:CDS
ACTCTATTTCCCGGATGACG+CGG 0.665825 1_4:-8226138 None:intergenic
TGATGATGATGATCAAGGGA+AGG 0.695681 1_4:+8224593 Msa0148040:CDS
GCTGAGAGTAAATATAATGG+TGG 0.695812 1_4:+8224711 Msa0148040:CDS
GATTATGGAGCTTGAAACGG+TGG 0.711095 1_4:+8224509 Msa0148040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGTATTTTTTTATTGTTT+TGG + chr1_4:8224816-8224835 Msa0148040:intron 10.0%
!!! ATTTTTAGTTAATTTAGTTA+GGG + chr1_4:8225045-8225064 Msa0148040:intron 10.0%
!!! ATTGTATTTTTTTATTGTTT+TGG + chr1_4:8224816-8224835 Msa0148040:intron 10.0%
!!! ATTTTTAGTTAATTTAGTTA+GGG + chr1_4:8225045-8225064 Msa0148040:intron 10.0%
!!! CAAAAATGGATTTATAATAA+TGG - chr1_4:8224457-8224476 None:intergenic 15.0%
!! AGGAAAAAAAATGTTAATAA+TGG - chr1_4:8224786-8224805 None:intergenic 15.0%
!!! TTTTTTTTAATGTTTTAAGC+AGG + chr1_4:8225000-8225019 Msa0148040:intron 15.0%
!!! TTTTTTTAATGTTTTAAGCA+GGG + chr1_4:8225001-8225020 Msa0148040:intron 15.0%
!!! CATTTTTAGTTAATTTAGTT+AGG + chr1_4:8225044-8225063 Msa0148040:intron 15.0%
!! GGTATTAGTTAAATTAATTA+TGG + chr1_4:8225127-8225146 Msa0148040:intron 15.0%
!! CAATTTGATTAAATGAAATT+TGG + chr1_4:8225164-8225183 Msa0148040:intron 15.0%
!!! TTTTTTGCTAAAAATAGTAA+GGG + chr1_4:8225306-8225325 Msa0148040:intron 15.0%
!!! GTTTAAAAGTTTTTATTCAA+TGG - chr1_4:8225696-8225715 None:intergenic 15.0%
!!! CAAAAATGGATTTATAATAA+TGG - chr1_4:8224457-8224476 None:intergenic 15.0%
!! AGGAAAAAAAATGTTAATAA+TGG - chr1_4:8224786-8224805 None:intergenic 15.0%
!!! TTTTTTTTAATGTTTTAAGC+AGG + chr1_4:8225000-8225019 Msa0148040:intron 15.0%
!!! TTTTTTTAATGTTTTAAGCA+GGG + chr1_4:8225001-8225020 Msa0148040:intron 15.0%
!!! CATTTTTAGTTAATTTAGTT+AGG + chr1_4:8225044-8225063 Msa0148040:intron 15.0%
!! GGTATTAGTTAAATTAATTA+TGG + chr1_4:8225127-8225146 Msa0148040:intron 15.0%
!! CAATTTGATTAAATGAAATT+TGG + chr1_4:8225164-8225183 Msa0148040:intron 15.0%
!!! TTTTTTGCTAAAAATAGTAA+GGG + chr1_4:8225306-8225325 Msa0148040:intron 15.0%
!!! GTTTAAAAGTTTTTATTCAA+TGG - chr1_4:8225696-8225715 None:intergenic 15.0%
!!! ATGGATTTATAATAATGGAA+AGG - chr1_4:8224452-8224471 None:intergenic 20.0%
!!! TCTTTTCATTTTGATGATTA+TGG + chr1_4:8224494-8224513 Msa0148040:exon 20.0%
!!! TTTGAGAAATGAATTTTTGT+GGG + chr1_4:8225105-8225124 Msa0148040:intron 20.0%
!! ATTAATTATGGAACTATTGT+TGG + chr1_4:8225139-8225158 Msa0148040:intron 20.0%
!!! GTTTTTTGCTAAAAATAGTA+AGG + chr1_4:8225305-8225324 Msa0148040:intron 20.0%
!! TTGAATACTAAAACTGAATA+TGG + chr1_4:8225485-8225504 Msa0148040:intron 20.0%
!!! TCATTTTCAATTGAAGAATT+TGG - chr1_4:8226249-8226268 None:intergenic 20.0%
!!! GTTGTATTTTAGTTATCATA+AGG - chr1_4:8226303-8226322 None:intergenic 20.0%
!!! TGTATAGTTGATGATATATA+CGG + chr1_4:8226437-8226456 Msa0148040:three_prime_UTR 20.0%
!!! ATGGATTTATAATAATGGAA+AGG - chr1_4:8224452-8224471 None:intergenic 20.0%
!!! TCTTTTCATTTTGATGATTA+TGG + chr1_4:8224494-8224513 Msa0148040:exon 20.0%
!!! TTTGAGAAATGAATTTTTGT+GGG + chr1_4:8225105-8225124 Msa0148040:intron 20.0%
!! ATTAATTATGGAACTATTGT+TGG + chr1_4:8225139-8225158 Msa0148040:intron 20.0%
!!! GTTTTTTGCTAAAAATAGTA+AGG + chr1_4:8225305-8225324 Msa0148040:intron 20.0%
!! TTGAATACTAAAACTGAATA+TGG + chr1_4:8225485-8225504 Msa0148040:intron 20.0%
!!! TCATTTTCAATTGAAGAATT+TGG - chr1_4:8226249-8226268 None:intergenic 20.0%
!!! GTTGTATTTTAGTTATCATA+AGG - chr1_4:8226303-8226322 None:intergenic 20.0%
!!! TGTATAGTTGATGATATATA+CGG + chr1_4:8226437-8226456 Msa0148040:three_prime_UTR 20.0%
! GTTAATAATGGTTACCTTAT+AGG - chr1_4:8224774-8224793 None:intergenic 25.0%
! AACAAAACATTCAAAAGTGA+AGG - chr1_4:8224882-8224901 None:intergenic 25.0%
!! GTTTGAGAAATGAATTTTTG+TGG + chr1_4:8225104-8225123 Msa0148040:intron 25.0%
!! TTGAGAAATGAATTTTTGTG+GGG + chr1_4:8225106-8225125 Msa0148040:intron 25.0%
!! AATTTGGTGATTGTATTGTA+AGG + chr1_4:8225180-8225199 Msa0148040:intron 25.0%
!! ACCTAATTTTGTGAAACAAA+GGG - chr1_4:8225268-8225287 None:intergenic 25.0%
!! AACCTAATTTTGTGAAACAA+AGG - chr1_4:8225269-8225288 None:intergenic 25.0%
!!! ACTTTTGATTGAAGAAAACT+TGG + chr1_4:8225401-8225420 Msa0148040:intron 25.0%
!!! CTTTTGATTGAAGAAAACTT+GGG + chr1_4:8225402-8225421 Msa0148040:intron 25.0%
!! TACTGGATAAACTTTTGAAT+TGG + chr1_4:8225461-8225480 Msa0148040:intron 25.0%
! AGTATCTCAAATTTCTATAG+CGG + chr1_4:8225805-8225824 Msa0148040:CDS 25.0%
!! TTTACAATGTCTTTTACAGA+TGG - chr1_4:8225874-8225893 None:intergenic 25.0%
!!! GTATAGTTGATGATATATAC+GGG + chr1_4:8226438-8226457 Msa0148040:three_prime_UTR 25.0%
! TCAAACCAACTATTATTTGT+TGG - chr1_4:8226502-8226521 None:intergenic 25.0%
!! TGATCTTACAATCTTTTCTT+TGG + chr1_4:8226519-8226538 Msa0148040:three_prime_UTR 25.0%
! GTTAATAATGGTTACCTTAT+AGG - chr1_4:8224774-8224793 None:intergenic 25.0%
! AACAAAACATTCAAAAGTGA+AGG - chr1_4:8224882-8224901 None:intergenic 25.0%
!! GTTTGAGAAATGAATTTTTG+TGG + chr1_4:8225104-8225123 Msa0148040:intron 25.0%
!! TTGAGAAATGAATTTTTGTG+GGG + chr1_4:8225106-8225125 Msa0148040:intron 25.0%
!! AATTTGGTGATTGTATTGTA+AGG + chr1_4:8225180-8225199 Msa0148040:intron 25.0%
!! ACCTAATTTTGTGAAACAAA+GGG - chr1_4:8225268-8225287 None:intergenic 25.0%
!! AACCTAATTTTGTGAAACAA+AGG - chr1_4:8225269-8225288 None:intergenic 25.0%
!!! ACTTTTGATTGAAGAAAACT+TGG + chr1_4:8225401-8225420 Msa0148040:intron 25.0%
!!! CTTTTGATTGAAGAAAACTT+GGG + chr1_4:8225402-8225421 Msa0148040:intron 25.0%
!! TACTGGATAAACTTTTGAAT+TGG + chr1_4:8225461-8225480 Msa0148040:intron 25.0%
! AGTATCTCAAATTTCTATAG+CGG + chr1_4:8225805-8225824 Msa0148040:CDS 25.0%
!! TTTACAATGTCTTTTACAGA+TGG - chr1_4:8225874-8225893 None:intergenic 25.0%
!!! GTATAGTTGATGATATATAC+GGG + chr1_4:8226438-8226457 Msa0148040:three_prime_UTR 25.0%
! TCAAACCAACTATTATTTGT+TGG - chr1_4:8226502-8226521 None:intergenic 25.0%
!! TGATCTTACAATCTTTTCTT+TGG + chr1_4:8226519-8226538 Msa0148040:three_prime_UTR 25.0%
TAGACACGAAAATGCAAAAA+TGG - chr1_4:8224471-8224490 None:intergenic 30.0%
!! TTGATGATGATGATGATCAA+GGG + chr1_4:8224589-8224608 Msa0148040:CDS 30.0%
! AAGAAATAGTGATGATGATG+AGG + chr1_4:8224647-8224666 Msa0148040:CDS 30.0%
GAAGCTGAGAGTAAATATAA+TGG + chr1_4:8224708-8224727 Msa0148040:CDS 30.0%
! TCCCTTTGTTTCACAAAATT+AGG + chr1_4:8225264-8225283 Msa0148040:intron 30.0%
! TAAGGGTTTCTGATTTGATA+TGG + chr1_4:8225323-8225342 Msa0148040:intron 30.0%
CCAAAACATGATGAATTCAT+TGG - chr1_4:8225355-8225374 None:intergenic 30.0%
! CCAATGAATTCATCATGTTT+TGG + chr1_4:8225352-8225371 Msa0148040:intron 30.0%
GTATGATTGAAGCTTACAAA+AGG - chr1_4:8225383-8225402 None:intergenic 30.0%
CTAAAGAACATGTTTGCAAT+TGG + chr1_4:8225438-8225457 Msa0148040:intron 30.0%
!!! AAACTGAATATGGCCTTTTT+TGG + chr1_4:8225495-8225514 Msa0148040:intron 30.0%
!!! AATATGGCCTTTTTTGGTTT+TGG + chr1_4:8225501-8225520 Msa0148040:intron 30.0%
TCCTTAACCAAAACCAAAAA+AGG - chr1_4:8225511-8225530 None:intergenic 30.0%
AAATGCACAATTGCACAATT+TGG - chr1_4:8225659-8225678 None:intergenic 30.0%
! AAGGCTAATCCAATCTTTTA+TGG + chr1_4:8225770-8225789 Msa0148040:intron 30.0%
GTATCTCAAATTTCTATAGC+GGG + chr1_4:8225806-8225825 Msa0148040:CDS 30.0%
!! TAAGGTTTTATACACTGTGA+TGG - chr1_4:8226285-8226304 None:intergenic 30.0%
! CCAACTATTATTTGTTGGTT+CGG - chr1_4:8226497-8226516 None:intergenic 30.0%
TAGACACGAAAATGCAAAAA+TGG - chr1_4:8224471-8224490 None:intergenic 30.0%
!! TTGATGATGATGATGATCAA+GGG + chr1_4:8224589-8224608 Msa0148040:CDS 30.0%
! AAGAAATAGTGATGATGATG+AGG + chr1_4:8224647-8224666 Msa0148040:CDS 30.0%
GAAGCTGAGAGTAAATATAA+TGG + chr1_4:8224708-8224727 Msa0148040:CDS 30.0%
! TCCCTTTGTTTCACAAAATT+AGG + chr1_4:8225264-8225283 Msa0148040:intron 30.0%
! TAAGGGTTTCTGATTTGATA+TGG + chr1_4:8225323-8225342 Msa0148040:intron 30.0%
CCAAAACATGATGAATTCAT+TGG - chr1_4:8225355-8225374 None:intergenic 30.0%
! CCAATGAATTCATCATGTTT+TGG + chr1_4:8225352-8225371 Msa0148040:intron 30.0%
GTATGATTGAAGCTTACAAA+AGG - chr1_4:8225383-8225402 None:intergenic 30.0%
CTAAAGAACATGTTTGCAAT+TGG + chr1_4:8225438-8225457 Msa0148040:intron 30.0%
!!! AAACTGAATATGGCCTTTTT+TGG + chr1_4:8225495-8225514 Msa0148040:intron 30.0%
!!! AATATGGCCTTTTTTGGTTT+TGG + chr1_4:8225501-8225520 Msa0148040:intron 30.0%
TCCTTAACCAAAACCAAAAA+AGG - chr1_4:8225511-8225530 None:intergenic 30.0%
AAATGCACAATTGCACAATT+TGG - chr1_4:8225659-8225678 None:intergenic 30.0%
! AAGGCTAATCCAATCTTTTA+TGG + chr1_4:8225770-8225789 Msa0148040:intron 30.0%
GTATCTCAAATTTCTATAGC+GGG + chr1_4:8225806-8225825 Msa0148040:CDS 30.0%
!! TAAGGTTTTATACACTGTGA+TGG - chr1_4:8226285-8226304 None:intergenic 30.0%
! CCAACTATTATTTGTTGGTT+CGG - chr1_4:8226497-8226516 None:intergenic 30.0%
GATGATTATGGAGCTTGAAA+CGG + chr1_4:8224506-8224525 Msa0148040:exon 35.0%
!! GTTGATGATGATGATGATCA+AGG + chr1_4:8224588-8224607 Msa0148040:CDS 35.0%
GCTGAGAGTAAATATAATGG+TGG + chr1_4:8224711-8224730 Msa0148040:CDS 35.0%
TAGAAGAGGTTCTTCCTATA+AGG + chr1_4:8224757-8224776 Msa0148040:CDS 35.0%
! TTAGTTAGGGTACTAGTTGT+TGG + chr1_4:8225058-8225077 Msa0148040:intron 35.0%
! TGATGGATGATGTGATCTAT+AGG + chr1_4:8225223-8225242 Msa0148040:intron 35.0%
AACATGTTTGCAATTGGTAC+TGG + chr1_4:8225444-8225463 Msa0148040:intron 35.0%
!!! GCCTTTTTTGGTTTTGGTTA+AGG + chr1_4:8225507-8225526 Msa0148040:intron 35.0%
CCTGCATAACCATAAAAGAT+TGG - chr1_4:8225782-8225801 None:intergenic 35.0%
! CCAATCTTTTATGGTTATGC+AGG + chr1_4:8225779-8225798 Msa0148040:intron 35.0%
AAGAAGACGTAGAAATTTGC+TGG + chr1_4:8225912-8225931 Msa0148040:CDS 35.0%
!! AATTTGCTGGCTTTCAATCA+TGG + chr1_4:8225925-8225944 Msa0148040:CDS 35.0%
AAAGAAGACACTCTATTTCC+CGG - chr1_4:8226150-8226169 None:intergenic 35.0%
GAAAAGAAATTCCTCTGCAA+AGG - chr1_4:8226180-8226199 None:intergenic 35.0%
! CATTTTCATTGTTGCAGCAA+TGG - chr1_4:8226226-8226245 None:intergenic 35.0%
! ATTTTCTTTCAGCGAGAAAG+AGG + chr1_4:8226359-8226378 Msa0148040:three_prime_UTR 35.0%
!! AGTTGATGATATATACGGGT+CGG + chr1_4:8226442-8226461 Msa0148040:three_prime_UTR 35.0%
CCGAACCAACAAATAATAGT+TGG + chr1_4:8226494-8226513 Msa0148040:three_prime_UTR 35.0%
!! TCTTTTCTTTGGAAAACCGA+TGG + chr1_4:8226530-8226549 Msa0148040:three_prime_UTR 35.0%
GATGATTATGGAGCTTGAAA+CGG + chr1_4:8224506-8224525 Msa0148040:exon 35.0%
!! GTTGATGATGATGATGATCA+AGG + chr1_4:8224588-8224607 Msa0148040:CDS 35.0%
GCTGAGAGTAAATATAATGG+TGG + chr1_4:8224711-8224730 Msa0148040:CDS 35.0%
TAGAAGAGGTTCTTCCTATA+AGG + chr1_4:8224757-8224776 Msa0148040:CDS 35.0%
! TTAGTTAGGGTACTAGTTGT+TGG + chr1_4:8225058-8225077 Msa0148040:intron 35.0%
! TGATGGATGATGTGATCTAT+AGG + chr1_4:8225223-8225242 Msa0148040:intron 35.0%
AACATGTTTGCAATTGGTAC+TGG + chr1_4:8225444-8225463 Msa0148040:intron 35.0%
!!! GCCTTTTTTGGTTTTGGTTA+AGG + chr1_4:8225507-8225526 Msa0148040:intron 35.0%
CCTGCATAACCATAAAAGAT+TGG - chr1_4:8225782-8225801 None:intergenic 35.0%
! CCAATCTTTTATGGTTATGC+AGG + chr1_4:8225779-8225798 Msa0148040:intron 35.0%
AAGAAGACGTAGAAATTTGC+TGG + chr1_4:8225912-8225931 Msa0148040:CDS 35.0%
!! AATTTGCTGGCTTTCAATCA+TGG + chr1_4:8225925-8225944 Msa0148040:CDS 35.0%
AAAGAAGACACTCTATTTCC+CGG - chr1_4:8226150-8226169 None:intergenic 35.0%
GAAAAGAAATTCCTCTGCAA+AGG - chr1_4:8226180-8226199 None:intergenic 35.0%
! CATTTTCATTGTTGCAGCAA+TGG - chr1_4:8226226-8226245 None:intergenic 35.0%
! ATTTTCTTTCAGCGAGAAAG+AGG + chr1_4:8226359-8226378 Msa0148040:three_prime_UTR 35.0%
!! AGTTGATGATATATACGGGT+CGG + chr1_4:8226442-8226461 Msa0148040:three_prime_UTR 35.0%
CCGAACCAACAAATAATAGT+TGG + chr1_4:8226494-8226513 Msa0148040:three_prime_UTR 35.0%
!! TCTTTTCTTTGGAAAACCGA+TGG + chr1_4:8226530-8226549 Msa0148040:three_prime_UTR 35.0%
TGTATTATTTGACGCGCGAA+GGG + chr1_4:8224560-8224579 Msa0148040:CDS 40.0%
! TGATGATGATGATCAAGGGA+AGG + chr1_4:8224593-8224612 Msa0148040:CDS 40.0%
GGTTTCATCAGAGAGATCAA+TGG + chr1_4:8224668-8224687 Msa0148040:CDS 40.0%
! TGGTGGTGCTTTAGATTGTA+TGG + chr1_4:8224728-8224747 Msa0148040:CDS 40.0%
! TGGTGCTTTAGATTGTATGG+AGG + chr1_4:8224731-8224750 Msa0148040:CDS 40.0%
!! TTGTATGGAGGCTTTAGAAG+AGG + chr1_4:8224743-8224762 Msa0148040:CDS 40.0%
GTCTATCCAGTAGTGTATGA+TGG + chr1_4:8225206-8225225 Msa0148040:intron 40.0%
CATCATCCATCATACACTAC+TGG - chr1_4:8225215-8225234 None:intergenic 40.0%
ACTCACAGCGATTACAAACA+AGG - chr1_4:8225555-8225574 None:intergenic 40.0%
AAGCTACGAAGTGTAGTTTC+TGG + chr1_4:8225600-8225619 Msa0148040:intron 40.0%
CAATTTGGATCGAGTGAGTT+TGG - chr1_4:8225644-8225663 None:intergenic 40.0%
AACATCAGCTAGGCTTGTAA+AGG - chr1_4:8225842-8225861 None:intergenic 40.0%
CTTCTTGTGTATGCATTCTC+TGG - chr1_4:8225898-8225917 None:intergenic 40.0%
! TTCCCTTTGAGAAGCAATAG+TGG + chr1_4:8225967-8225986 Msa0148040:CDS 40.0%
CACCACTATTGCTTCTCAAA+GGG - chr1_4:8225972-8225991 None:intergenic 40.0%
!! AGAAGGTGCTGAAGAATATG+AGG - chr1_4:8226106-8226125 None:intergenic 40.0%
! GCAGAGGAATTTCTTTTCCT+TGG + chr1_4:8226182-8226201 Msa0148040:CDS 40.0%
TTGGAAAACCGATGGAAAGA+TGG + chr1_4:8226538-8226557 Msa0148040:three_prime_UTR 40.0%
AAACCGATGGAAAGATGGTT+TGG + chr1_4:8226543-8226562 Msa0148040:three_prime_UTR 40.0%
TGTATTATTTGACGCGCGAA+GGG + chr1_4:8224560-8224579 Msa0148040:CDS 40.0%
! TGATGATGATGATCAAGGGA+AGG + chr1_4:8224593-8224612 Msa0148040:CDS 40.0%
GGTTTCATCAGAGAGATCAA+TGG + chr1_4:8224668-8224687 Msa0148040:CDS 40.0%
! TGGTGGTGCTTTAGATTGTA+TGG + chr1_4:8224728-8224747 Msa0148040:CDS 40.0%
! TGGTGCTTTAGATTGTATGG+AGG + chr1_4:8224731-8224750 Msa0148040:CDS 40.0%
!! TTGTATGGAGGCTTTAGAAG+AGG + chr1_4:8224743-8224762 Msa0148040:CDS 40.0%
GTCTATCCAGTAGTGTATGA+TGG + chr1_4:8225206-8225225 Msa0148040:intron 40.0%
CATCATCCATCATACACTAC+TGG - chr1_4:8225215-8225234 None:intergenic 40.0%
ACTCACAGCGATTACAAACA+AGG - chr1_4:8225555-8225574 None:intergenic 40.0%
AAGCTACGAAGTGTAGTTTC+TGG + chr1_4:8225600-8225619 Msa0148040:intron 40.0%
CAATTTGGATCGAGTGAGTT+TGG - chr1_4:8225644-8225663 None:intergenic 40.0%
AACATCAGCTAGGCTTGTAA+AGG - chr1_4:8225842-8225861 None:intergenic 40.0%
CTTCTTGTGTATGCATTCTC+TGG - chr1_4:8225898-8225917 None:intergenic 40.0%
! TTCCCTTTGAGAAGCAATAG+TGG + chr1_4:8225967-8225986 Msa0148040:CDS 40.0%
CACCACTATTGCTTCTCAAA+GGG - chr1_4:8225972-8225991 None:intergenic 40.0%
!! AGAAGGTGCTGAAGAATATG+AGG - chr1_4:8226106-8226125 None:intergenic 40.0%
! GCAGAGGAATTTCTTTTCCT+TGG + chr1_4:8226182-8226201 Msa0148040:CDS 40.0%
TTGGAAAACCGATGGAAAGA+TGG + chr1_4:8226538-8226557 Msa0148040:three_prime_UTR 40.0%
AAACCGATGGAAAGATGGTT+TGG + chr1_4:8226543-8226562 Msa0148040:three_prime_UTR 40.0%
GATTATGGAGCTTGAAACGG+TGG + chr1_4:8224509-8224528 Msa0148040:CDS 45.0%
CTGTATTATTTGACGCGCGA+AGG + chr1_4:8224559-8224578 Msa0148040:CDS 45.0%
GAGAGATCAATGGACGAGAA+TGG + chr1_4:8224678-8224697 Msa0148040:CDS 45.0%
! CTACGAAGTGTAGTTTCTGG+TGG + chr1_4:8225603-8225622 Msa0148040:intron 45.0%
GTGTTGTGACAACATCAGCT+AGG - chr1_4:8225852-8225871 None:intergenic 45.0%
!! TGCTGGCTTTCAATCATGGT+TGG + chr1_4:8225929-8225948 Msa0148040:CDS 45.0%
!! GCTGGCTTTCAATCATGGTT+GGG + chr1_4:8225930-8225949 Msa0148040:CDS 45.0%
CCACCACTATTGCTTCTCAA+AGG - chr1_4:8225973-8225992 None:intergenic 45.0%
! CCTTTGAGAAGCAATAGTGG+TGG + chr1_4:8225970-8225989 Msa0148040:CDS 45.0%
CTTTAGCATCTCCTTTGCAG+AGG + chr1_4:8226166-8226185 Msa0148040:CDS 45.0%
ACAATGATGCAGATCAGCCA+AGG - chr1_4:8226202-8226221 None:intergenic 45.0%
GATTATGGAGCTTGAAACGG+TGG + chr1_4:8224509-8224528 Msa0148040:CDS 45.0%
CTGTATTATTTGACGCGCGA+AGG + chr1_4:8224559-8224578 Msa0148040:CDS 45.0%
GAGAGATCAATGGACGAGAA+TGG + chr1_4:8224678-8224697 Msa0148040:CDS 45.0%
! CTACGAAGTGTAGTTTCTGG+TGG + chr1_4:8225603-8225622 Msa0148040:intron 45.0%
GTGTTGTGACAACATCAGCT+AGG - chr1_4:8225852-8225871 None:intergenic 45.0%
!! TGCTGGCTTTCAATCATGGT+TGG + chr1_4:8225929-8225948 Msa0148040:CDS 45.0%
!! GCTGGCTTTCAATCATGGTT+GGG + chr1_4:8225930-8225949 Msa0148040:CDS 45.0%
CCACCACTATTGCTTCTCAA+AGG - chr1_4:8225973-8225992 None:intergenic 45.0%
! CCTTTGAGAAGCAATAGTGG+TGG + chr1_4:8225970-8225989 Msa0148040:CDS 45.0%
CTTTAGCATCTCCTTTGCAG+AGG + chr1_4:8226166-8226185 Msa0148040:CDS 45.0%
ACAATGATGCAGATCAGCCA+AGG - chr1_4:8226202-8226221 None:intergenic 45.0%
!! AAAATACAATATAATATAAT+AGG - chr1_4:8224806-8224825 None:intergenic 5.0%
!!! TAATTTTTTGTAAATTATAA+AGG + chr1_4:8225751-8225770 Msa0148040:intron 5.0%
!! AAAATACAATATAATATAAT+AGG - chr1_4:8224806-8224825 None:intergenic 5.0%
!!! TAATTTTTTGTAAATTATAA+AGG + chr1_4:8225751-8225770 Msa0148040:intron 5.0%
ACGACGTCGTCTTCATCGAT+CGG + chr1_4:8224624-8224643 Msa0148040:CDS 50.0%
ACTCTATTTCCCGGATGACG+CGG - chr1_4:8226141-8226160 None:intergenic 50.0%
ACGACGTCGTCTTCATCGAT+CGG + chr1_4:8224624-8224643 Msa0148040:CDS 50.0%
ACTCTATTTCCCGGATGACG+CGG - chr1_4:8226141-8226160 None:intergenic 50.0%
AACGGTGGCGTTTAAGCGCG+TGG + chr1_4:8224524-8224543 Msa0148040:CDS 60.0%
ACGGTGGCGTTTAAGCGCGT+GGG + chr1_4:8224525-8224544 Msa0148040:CDS 60.0%
CTATTTCCCGGATGACGCGG+TGG - chr1_4:8226138-8226157 None:intergenic 60.0%
AACGGTGGCGTTTAAGCGCG+TGG + chr1_4:8224524-8224543 Msa0148040:CDS 60.0%
ACGGTGGCGTTTAAGCGCGT+GGG + chr1_4:8224525-8224544 Msa0148040:CDS 60.0%
CTATTTCCCGGATGACGCGG+TGG - chr1_4:8226138-8226157 None:intergenic 60.0%
CGCGGTGGAAGTGGCGAAGA+AGG - chr1_4:8226123-8226142 None:intergenic 65.0%
GCCACTTCCACCGCGTCATC+CGG + chr1_4:8226128-8226147 Msa0148040:CDS 65.0%
CCACTTCCACCGCGTCATCC+GGG + chr1_4:8226129-8226148 Msa0148040:CDS 65.0%
CGCGGTGGAAGTGGCGAAGA+AGG - chr1_4:8226123-8226142 None:intergenic 65.0%
GCCACTTCCACCGCGTCATC+CGG + chr1_4:8226128-8226147 Msa0148040:CDS 65.0%
CCACTTCCACCGCGTCATCC+GGG + chr1_4:8226129-8226148 Msa0148040:CDS 65.0%
CCCGGATGACGCGGTGGAAG+TGG - chr1_4:8226132-8226151 None:intergenic 70.0%
CCCGGATGACGCGGTGGAAG+TGG - chr1_4:8226132-8226151 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 8224432 8226566 8224432 ID=Msa0148040;Name=Msa0148040
chr1_4 mRNA 8224432 8226566 8224432 ID=Msa0148040-mRNA-1;Parent=Msa0148040;Name=Msa0148040-mRNA-1;_AED=0.01;_eAED=0.01;_QI=75|1|1|1|1|1|2|252|261
chr1_4 exon 8224432 8224778 8224432 ID=Msa0148040-mRNA-1:exon:3524;Parent=Msa0148040-mRNA-1
chr1_4 exon 8225801 8226566 8225801 ID=Msa0148040-mRNA-1:exon:3525;Parent=Msa0148040-mRNA-1
chr1_4 five_prime_UTR 8224432 8224506 8224432 ID=Msa0148040-mRNA-1:five_prime_utr;Parent=Msa0148040-mRNA-1
chr1_4 CDS 8224507 8224778 8224507 ID=Msa0148040-mRNA-1:cds;Parent=Msa0148040-mRNA-1
chr1_4 CDS 8225801 8226314 8225801 ID=Msa0148040-mRNA-1:cds;Parent=Msa0148040-mRNA-1
chr1_4 three_prime_UTR 8226315 8226566 8226315 ID=Msa0148040-mRNA-1:three_prime_utr;Parent=Msa0148040-mRNA-1
Gene Sequence

>Msa0148040

ATGATTATGGAGCTTGAAACGGTGGCGTTTAAGCGCGTGGGATCACGTACTTCTGTATTATTTGACGCGCGAAGGGATCATGTTGATGATGATGATGATCAAGGGAAGGTGTGTAGTACGACGTCGTCTTCATCGATCGGAAGAAATAGTGATGATGATGAGGTTTCATCAGAGAGATCAATGGACGAGAATGGTGAAAATGAAGCTGAGAGTAAATATAATGGTGGTGCTTTAGATTGTATGGAGGCTTTAGAAGAGGTTCTTCCTATAAGGAGAAGTATCTCAAATTTCTATAGCGGGAAGTCTAAGTCCTTTACAAGCCTAGCTGATGTTGTCACAACACCATCTGTAAAAGACATTGTAAAACCAGAGAATGCATACACAAGAAGACGTAGAAATTTGCTGGCTTTCAATCATGGTTGGGACAAGAACAAAAATTTCCCTTTGAGAAGCAATAGTGGTGGAATTTCAAAGAGAACAATGAGCTTAAGTAGAAGTGCTTTAGCTCTTGCAGTTGCATTAAGCAATTCTGATAGTAGCTCTAGTTTTACAAGTGATGATTCAGCTGCTTCAACCTCATATTCTTCAGCACCTTCTTCGCCACTTCCACCGCGTCATCCGGGAAATAGAGTGTCTTCTTTAGCATCTCCTTTGCAGAGGAATTTCTTTTCCTTGGCTGATCTGCATCATTGTGCCATTGCTGCAACAATGAAAATGCCAAATTCTTCAATTGAAAATGAAACAACTAATCATCCATCACAGTGTATAAAACCTTATGATAACTAA
Protein sequence

>Msa0148040

MIMELETVAFKRVGSRTSVLFDARRDHVDDDDDQGKVCSTTSSSSIGRNSDDDEVSSERSMDENGENEAESKYNGGALDCMEALEEVLPIRRSISNFYSGKSKSFTSLADVVTTPSVKDIVKPENAYTRRRRNLLAFNHGWDKNKNFPLRSNSGGISKRTMSLSRSALALAVALSNSDSSSSFTSDDSAASTSYSSAPSSPLPPRHPGNRVSSLASPLQRNFFSLADLHHCAIAATMKMPNSSIENETTNHPSQCIKPYDN*