AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0053640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053640 MtrunA17_Chr1g0151071 96.063 254 4 2 3 256 1 248 2.86e-175 482
Msa0053640 MtrunA17_Chr3g0136361 51.685 178 69 4 1 177 1 162 9.09e-53 170
Msa0053640 MtrunA17_Chr4g0070451 41.916 167 78 7 79 235 66 223 3.39e-23 94.7
Msa0053640 MtrunA17_Chr0c27g0493891 41.060 151 70 7 95 235 3 144 5.60e-17 76.3
Msa0053640 MtrunA17_Chr1g0168551 43.182 88 44 3 52 139 46 127 9.44e-15 70.5
Msa0053640 MtrunA17_Chr1g0147921 42.105 95 49 3 46 139 43 132 1.73e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053640 AT5G21940.1 53.957 139 49 6 46 178 44 173 8.53e-33 120
Msa0053640 AT3G43850.2 49.612 129 37 5 46 174 30 130 5.25e-27 103
Msa0053640 AT3G43850.1 49.612 129 37 5 46 174 30 130 1.44e-26 104

Find 52 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAATAATGGTTACCTTAT+AGG 0.166825 1_2:-8117486 None:intergenic
TGTTTGTATATTTATAATAA+TGG 0.261116 1_2:-8117148 None:intergenic
TAGAAGAGGTTCTTCCTATA+AGG 0.279952 1_2:+8117472 Msa0053640:CDS
TGTATAGTTGATGATATATA+CGG 0.285224 1_2:+8119158 Msa0053640:three_prime_UTR
GCCACTTCCACCGCGTCATC+CGG 0.302841 1_2:+8118847 Msa0053640:CDS
CCAACTATTATTTGTTGGTT+CGG 0.374739 1_2:-8119215 None:intergenic
CTTCTTGTGTATGCATTCTC+TGG 0.380516 1_2:-8118614 None:intergenic
TCAAACCAACTATTATTTGT+TGG 0.400637 1_2:-8119220 None:intergenic
TGGTGGTGCTTTAGATTGTA+TGG 0.406572 1_2:+8117443 Msa0053640:CDS
AATTTGCTGGCTTTCAATCA+TGG 0.423862 1_2:+8118644 Msa0053640:CDS
AAGAAGACGTAGAAATTTGC+TGG 0.426140 1_2:+8118631 Msa0053640:CDS
GCTGGCTTTCAATCATGGTT+GGG 0.432602 1_2:+8118649 Msa0053640:CDS
AAAGAAGACACTCTATTTCC+CGG 0.439102 1_2:-8118866 None:intergenic
CCGAACCAACAAATAATAGT+TGG 0.439930 1_2:+8119215 Msa0053640:three_prime_UTR
CGCGGTGGAAGTGGCGAAGA+AGG 0.445270 1_2:-8118839 None:intergenic
GAAGCTGAGAGTAAATATAA+TGG 0.445348 1_2:+8117423 Msa0053640:CDS
TGCTGGCTTTCAATCATGGT+TGG 0.445425 1_2:+8118648 Msa0053640:CDS
TTCCCTTTGAGAAGCAATAG+TGG 0.449201 1_2:+8118686 Msa0053640:CDS
AAACCGATGGAAAGATGGTT+TGG 0.451187 1_2:+8119264 Msa0053640:three_prime_UTR
CCCGGATGACGCGGTGGAAG+TGG 0.453467 1_2:-8118848 None:intergenic
CTGTATTATTTGACGCGCGA+AGG 0.467561 1_2:+8117265 Msa0053640:CDS
TTGGAAAACCGATGGAAAGA+TGG 0.469621 1_2:+8119259 Msa0053640:three_prime_UTR
CACCACTATTGCTTCTCAAA+GGG 0.472099 1_2:-8118688 None:intergenic
TGTATTATTTGACGCGCGAA+GGG 0.506332 1_2:+8117266 Msa0053640:CDS
CCACCACTATTGCTTCTCAA+AGG 0.509782 1_2:-8118689 None:intergenic
GTATCTCAAATTTCTATAGC+GGG 0.512706 1_2:+8118525 Msa0053640:CDS
CCACTTCCACCGCGTCATCC+GGG 0.531769 1_2:+8118848 Msa0053640:CDS
AACGGTGGCGTTTAAGCGCG+TGG 0.535037 1_2:+8117230 Msa0053640:CDS
AGAAGGTGCTGAAGAATATG+AGG 0.542161 1_2:-8118822 None:intergenic
GAGAGATCAATGGATGAGAA+TGG 0.545446 1_2:+8117393 Msa0053640:CDS
GAAAAGAAATTCCTCTGCAA+AGG 0.546535 1_2:-8118896 None:intergenic
GTATATTTATAATAATGGAA+AGG 0.553135 1_2:-8117143 None:intergenic
GATGATTATGGAGCTTGAAA+CGG 0.556496 1_2:+8117212 Msa0053640:exon
AACATCAGCTAGGCTTGTAA+AGG 0.557041 1_2:-8118558 None:intergenic
ATACCAAACCATCTTTCCAT+CGG 0.566229 1_2:-8119267 None:intergenic
CTTTAGCATCTCCTTTGCAG+AGG 0.570257 1_2:+8118885 Msa0053640:CDS
TTGTATGGAGGCTTTAGAAG+AGG 0.575224 1_2:+8117458 Msa0053640:CDS
GTATAGTTGATGATATATAC+GGG 0.584796 1_2:+8119159 Msa0053640:three_prime_UTR
GGTTTCATCAGAGAGATCAA+TGG 0.590893 1_2:+8117383 Msa0053640:CDS
AGTATCTCAAATTTCTATAG+CGG 0.596091 1_2:+8118524 Msa0053640:CDS
CCTTTGAGAAGCAATAGTGG+TGG 0.600401 1_2:+8118689 Msa0053640:CDS
AGTTGATGATATATACGGGT+CGG 0.610096 1_2:+8119163 Msa0053640:three_prime_UTR
CTATTTCCCGGATGACGCGG+TGG 0.613883 1_2:-8118854 None:intergenic
ACAATGATGCAGATCAGCCA+AGG 0.617708 1_2:-8118918 None:intergenic
ACGACGTCGTCTTCATCGAT+CGG 0.620053 1_2:+8117330 Msa0053640:CDS
GTGTTGTGACAACATCAGCT+AGG 0.640480 1_2:-8118568 None:intergenic
ACGGTGGCGTTTAAGCGCGT+GGG 0.660985 1_2:+8117231 Msa0053640:CDS
TGGTGCTTTAGATTGTATGG+AGG 0.662476 1_2:+8117446 Msa0053640:CDS
ACTCTATTTCCCGGATGACG+CGG 0.665825 1_2:-8118857 None:intergenic
TGATGATGATGATCAAGGGA+AGG 0.695681 1_2:+8117299 Msa0053640:CDS
GCTGAGAGTAAATATAATGG+TGG 0.695812 1_2:+8117426 Msa0053640:CDS
GATTATGGAGCTTGAAACGG+TGG 0.711095 1_2:+8117215 Msa0053640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTGTATATTTATAATAA+TGG - chr1_2:8117151-8117170 None:intergenic 10.0%
!!! ATTGTATTTTTTTATTGTTT+TGG + chr1_2:8117531-8117550 Msa0053640:intron 10.0%
!!! ATTTTTAGTTAATTTAGTTA+GGG + chr1_2:8117764-8117783 Msa0053640:intron 10.0%
!! TGTTTGTATATTTATAATAA+TGG - chr1_2:8117151-8117170 None:intergenic 10.0%
!!! ATTGTATTTTTTTATTGTTT+TGG + chr1_2:8117531-8117550 Msa0053640:intron 10.0%
!!! ATTTTTAGTTAATTTAGTTA+GGG + chr1_2:8117764-8117783 Msa0053640:intron 10.0%
!!! GTATATTTATAATAATGGAA+AGG - chr1_2:8117146-8117165 None:intergenic 15.0%
!! AGGAAAAAAAATGTTAATAA+TGG - chr1_2:8117501-8117520 None:intergenic 15.0%
!!! TTTTTTTTAATGTTTTAAGC+AGG + chr1_2:8117719-8117738 Msa0053640:intron 15.0%
!!! TTTTTTTAATGTTTTAAGCA+GGG + chr1_2:8117720-8117739 Msa0053640:intron 15.0%
!!! CATTTTTAGTTAATTTAGTT+AGG + chr1_2:8117763-8117782 Msa0053640:intron 15.0%
!! GGTATTAGTTAAATTAATTA+TGG + chr1_2:8117846-8117865 Msa0053640:intron 15.0%
!! CAATTTGATTAAATGAAATT+TGG + chr1_2:8117883-8117902 Msa0053640:intron 15.0%
!!! TTTTTTGCTAAAAATAGTAA+GGG + chr1_2:8118025-8118044 Msa0053640:intron 15.0%
!!! GTTTAAAAGTTTTTATTCAA+TGG - chr1_2:8118415-8118434 None:intergenic 15.0%
!!! GTATATTTATAATAATGGAA+AGG - chr1_2:8117146-8117165 None:intergenic 15.0%
!! AGGAAAAAAAATGTTAATAA+TGG - chr1_2:8117501-8117520 None:intergenic 15.0%
!!! TTTTTTTTAATGTTTTAAGC+AGG + chr1_2:8117719-8117738 Msa0053640:intron 15.0%
!!! TTTTTTTAATGTTTTAAGCA+GGG + chr1_2:8117720-8117739 Msa0053640:intron 15.0%
!!! CATTTTTAGTTAATTTAGTT+AGG + chr1_2:8117763-8117782 Msa0053640:intron 15.0%
!! GGTATTAGTTAAATTAATTA+TGG + chr1_2:8117846-8117865 Msa0053640:intron 15.0%
!! CAATTTGATTAAATGAAATT+TGG + chr1_2:8117883-8117902 Msa0053640:intron 15.0%
!!! TTTTTTGCTAAAAATAGTAA+GGG + chr1_2:8118025-8118044 Msa0053640:intron 15.0%
!!! GTTTAAAAGTTTTTATTCAA+TGG - chr1_2:8118415-8118434 None:intergenic 15.0%
!!! TCTTTTCATTTTGATGATTA+TGG + chr1_2:8117200-8117219 Msa0053640:exon 20.0%
!!! TTTGAGAAATGAATTTTTGT+GGG + chr1_2:8117824-8117843 Msa0053640:intron 20.0%
!! ATTAATTATGGAACTATTGT+TGG + chr1_2:8117858-8117877 Msa0053640:intron 20.0%
!!! GTTTTTTGCTAAAAATAGTA+AGG + chr1_2:8118024-8118043 Msa0053640:intron 20.0%
!! TTGAATACTAAAACTGAATA+TGG + chr1_2:8118204-8118223 Msa0053640:intron 20.0%
!!! TCATTTTCAATTGAAGAATT+TGG - chr1_2:8118968-8118987 None:intergenic 20.0%
!!! GTTGTATTTTAGTTATCATA+AGG - chr1_2:8119022-8119041 None:intergenic 20.0%
!!! TGTATAGTTGATGATATATA+CGG + chr1_2:8119158-8119177 Msa0053640:three_prime_UTR 20.0%
!!! TCTTTTCATTTTGATGATTA+TGG + chr1_2:8117200-8117219 Msa0053640:exon 20.0%
!!! TTTGAGAAATGAATTTTTGT+GGG + chr1_2:8117824-8117843 Msa0053640:intron 20.0%
!! ATTAATTATGGAACTATTGT+TGG + chr1_2:8117858-8117877 Msa0053640:intron 20.0%
!!! GTTTTTTGCTAAAAATAGTA+AGG + chr1_2:8118024-8118043 Msa0053640:intron 20.0%
!! TTGAATACTAAAACTGAATA+TGG + chr1_2:8118204-8118223 Msa0053640:intron 20.0%
!!! TCATTTTCAATTGAAGAATT+TGG - chr1_2:8118968-8118987 None:intergenic 20.0%
!!! GTTGTATTTTAGTTATCATA+AGG - chr1_2:8119022-8119041 None:intergenic 20.0%
!!! TGTATAGTTGATGATATATA+CGG + chr1_2:8119158-8119177 Msa0053640:three_prime_UTR 20.0%
! GTTAATAATGGTTACCTTAT+AGG - chr1_2:8117489-8117508 None:intergenic 25.0%
! AACAAAACATTCAAAAGTGA+AGG - chr1_2:8117597-8117616 None:intergenic 25.0%
!! GTTTGAGAAATGAATTTTTG+TGG + chr1_2:8117823-8117842 Msa0053640:intron 25.0%
!! TTGAGAAATGAATTTTTGTG+GGG + chr1_2:8117825-8117844 Msa0053640:intron 25.0%
!! AATTTGGTGATTGTATTGTA+AGG + chr1_2:8117899-8117918 Msa0053640:intron 25.0%
!! ACCTAATTTTGTGAAACAAA+GGG - chr1_2:8117987-8118006 None:intergenic 25.0%
!! AACCTAATTTTGTGAAACAA+AGG - chr1_2:8117988-8118007 None:intergenic 25.0%
!!! ACTTTTGATTGAAGAAAACT+TGG + chr1_2:8118120-8118139 Msa0053640:intron 25.0%
!!! CTTTTGATTGAAGAAAACTT+GGG + chr1_2:8118121-8118140 Msa0053640:intron 25.0%
!! TACTGGATAAACTTTTGAAT+TGG + chr1_2:8118180-8118199 Msa0053640:intron 25.0%
! AGTATCTCAAATTTCTATAG+CGG + chr1_2:8118524-8118543 Msa0053640:CDS 25.0%
!! TTTACAATGTCTTTTACAGA+TGG - chr1_2:8118593-8118612 None:intergenic 25.0%
!!! GTATAGTTGATGATATATAC+GGG + chr1_2:8119159-8119178 Msa0053640:three_prime_UTR 25.0%
! TCAAACCAACTATTATTTGT+TGG - chr1_2:8119223-8119242 None:intergenic 25.0%
!! TGATCTTACAATCTTTTCTT+TGG + chr1_2:8119240-8119259 Msa0053640:three_prime_UTR 25.0%
! GTTAATAATGGTTACCTTAT+AGG - chr1_2:8117489-8117508 None:intergenic 25.0%
! AACAAAACATTCAAAAGTGA+AGG - chr1_2:8117597-8117616 None:intergenic 25.0%
!! GTTTGAGAAATGAATTTTTG+TGG + chr1_2:8117823-8117842 Msa0053640:intron 25.0%
!! TTGAGAAATGAATTTTTGTG+GGG + chr1_2:8117825-8117844 Msa0053640:intron 25.0%
!! AATTTGGTGATTGTATTGTA+AGG + chr1_2:8117899-8117918 Msa0053640:intron 25.0%
!! ACCTAATTTTGTGAAACAAA+GGG - chr1_2:8117987-8118006 None:intergenic 25.0%
!! AACCTAATTTTGTGAAACAA+AGG - chr1_2:8117988-8118007 None:intergenic 25.0%
!!! ACTTTTGATTGAAGAAAACT+TGG + chr1_2:8118120-8118139 Msa0053640:intron 25.0%
!!! CTTTTGATTGAAGAAAACTT+GGG + chr1_2:8118121-8118140 Msa0053640:intron 25.0%
!! TACTGGATAAACTTTTGAAT+TGG + chr1_2:8118180-8118199 Msa0053640:intron 25.0%
! AGTATCTCAAATTTCTATAG+CGG + chr1_2:8118524-8118543 Msa0053640:CDS 25.0%
!! TTTACAATGTCTTTTACAGA+TGG - chr1_2:8118593-8118612 None:intergenic 25.0%
!!! GTATAGTTGATGATATATAC+GGG + chr1_2:8119159-8119178 Msa0053640:three_prime_UTR 25.0%
! TCAAACCAACTATTATTTGT+TGG - chr1_2:8119223-8119242 None:intergenic 25.0%
!! TGATCTTACAATCTTTTCTT+TGG + chr1_2:8119240-8119259 Msa0053640:three_prime_UTR 25.0%
TAGACACGAAAATGCAAAAA+TGG - chr1_2:8117177-8117196 None:intergenic 30.0%
!! TTGATGATGATGATGATCAA+GGG + chr1_2:8117295-8117314 Msa0053640:CDS 30.0%
GAAGCTGAGAGTAAATATAA+TGG + chr1_2:8117423-8117442 Msa0053640:CDS 30.0%
! TCCCTTTGTTTCACAAAATT+AGG + chr1_2:8117983-8118002 Msa0053640:intron 30.0%
! TAAGGGTTTCTGATTTGATA+TGG + chr1_2:8118042-8118061 Msa0053640:intron 30.0%
CCAAAACATGATGAATTCAT+TGG - chr1_2:8118074-8118093 None:intergenic 30.0%
! CCAATGAATTCATCATGTTT+TGG + chr1_2:8118071-8118090 Msa0053640:intron 30.0%
GTATGATTGAAGCTTACAAA+AGG - chr1_2:8118102-8118121 None:intergenic 30.0%
CTAAAGAACATGTTTGCAAT+TGG + chr1_2:8118157-8118176 Msa0053640:intron 30.0%
!!! AAACTGAATATGGCCTTTTT+TGG + chr1_2:8118214-8118233 Msa0053640:intron 30.0%
!!! AATATGGCCTTTTTTGGTTT+TGG + chr1_2:8118220-8118239 Msa0053640:intron 30.0%
TCCTTAACCAAAACCAAAAA+AGG - chr1_2:8118230-8118249 None:intergenic 30.0%
AAATGCACAATTGCACAATT+TGG - chr1_2:8118378-8118397 None:intergenic 30.0%
! AAGGCTAATCCAATCTTTTA+TGG + chr1_2:8118489-8118508 Msa0053640:intron 30.0%
GTATCTCAAATTTCTATAGC+GGG + chr1_2:8118525-8118544 Msa0053640:CDS 30.0%
!! TAAGGTTTTATACACTGTGA+TGG - chr1_2:8119004-8119023 None:intergenic 30.0%
! CCAACTATTATTTGTTGGTT+CGG - chr1_2:8119218-8119237 None:intergenic 30.0%
TAGACACGAAAATGCAAAAA+TGG - chr1_2:8117177-8117196 None:intergenic 30.0%
!! TTGATGATGATGATGATCAA+GGG + chr1_2:8117295-8117314 Msa0053640:CDS 30.0%
GAAGCTGAGAGTAAATATAA+TGG + chr1_2:8117423-8117442 Msa0053640:CDS 30.0%
! TCCCTTTGTTTCACAAAATT+AGG + chr1_2:8117983-8118002 Msa0053640:intron 30.0%
! TAAGGGTTTCTGATTTGATA+TGG + chr1_2:8118042-8118061 Msa0053640:intron 30.0%
CCAAAACATGATGAATTCAT+TGG - chr1_2:8118074-8118093 None:intergenic 30.0%
! CCAATGAATTCATCATGTTT+TGG + chr1_2:8118071-8118090 Msa0053640:intron 30.0%
GTATGATTGAAGCTTACAAA+AGG - chr1_2:8118102-8118121 None:intergenic 30.0%
CTAAAGAACATGTTTGCAAT+TGG + chr1_2:8118157-8118176 Msa0053640:intron 30.0%
!!! AAACTGAATATGGCCTTTTT+TGG + chr1_2:8118214-8118233 Msa0053640:intron 30.0%
!!! AATATGGCCTTTTTTGGTTT+TGG + chr1_2:8118220-8118239 Msa0053640:intron 30.0%
TCCTTAACCAAAACCAAAAA+AGG - chr1_2:8118230-8118249 None:intergenic 30.0%
AAATGCACAATTGCACAATT+TGG - chr1_2:8118378-8118397 None:intergenic 30.0%
! AAGGCTAATCCAATCTTTTA+TGG + chr1_2:8118489-8118508 Msa0053640:intron 30.0%
GTATCTCAAATTTCTATAGC+GGG + chr1_2:8118525-8118544 Msa0053640:CDS 30.0%
!! TAAGGTTTTATACACTGTGA+TGG - chr1_2:8119004-8119023 None:intergenic 30.0%
! CCAACTATTATTTGTTGGTT+CGG - chr1_2:8119218-8119237 None:intergenic 30.0%
GATGATTATGGAGCTTGAAA+CGG + chr1_2:8117212-8117231 Msa0053640:exon 35.0%
!! GTTGATGATGATGATGATCA+AGG + chr1_2:8117294-8117313 Msa0053640:CDS 35.0%
! TGATGATGATGATGATGATG+AGG + chr1_2:8117362-8117381 Msa0053640:CDS 35.0%
GCTGAGAGTAAATATAATGG+TGG + chr1_2:8117426-8117445 Msa0053640:CDS 35.0%
TAGAAGAGGTTCTTCCTATA+AGG + chr1_2:8117472-8117491 Msa0053640:CDS 35.0%
! TTAGTTAGGGTACTAGTTGT+TGG + chr1_2:8117777-8117796 Msa0053640:intron 35.0%
! TGATGGATGATGTGATCTAT+AGG + chr1_2:8117942-8117961 Msa0053640:intron 35.0%
AACATGTTTGCAATTGGTAC+TGG + chr1_2:8118163-8118182 Msa0053640:intron 35.0%
!!! GCCTTTTTTGGTTTTGGTTA+AGG + chr1_2:8118226-8118245 Msa0053640:intron 35.0%
CCTGCATAACCATAAAAGAT+TGG - chr1_2:8118501-8118520 None:intergenic 35.0%
! CCAATCTTTTATGGTTATGC+AGG + chr1_2:8118498-8118517 Msa0053640:intron 35.0%
AAGAAGACGTAGAAATTTGC+TGG + chr1_2:8118631-8118650 Msa0053640:CDS 35.0%
!! AATTTGCTGGCTTTCAATCA+TGG + chr1_2:8118644-8118663 Msa0053640:CDS 35.0%
AAAGAAGACACTCTATTTCC+CGG - chr1_2:8118869-8118888 None:intergenic 35.0%
GAAAAGAAATTCCTCTGCAA+AGG - chr1_2:8118899-8118918 None:intergenic 35.0%
! CATTTTCATTGTTGCAGCAA+TGG - chr1_2:8118945-8118964 None:intergenic 35.0%
! ATTTTCTTTCAGCGAGAAAG+AGG + chr1_2:8119080-8119099 Msa0053640:three_prime_UTR 35.0%
!! AGTTGATGATATATACGGGT+CGG + chr1_2:8119163-8119182 Msa0053640:three_prime_UTR 35.0%
CCGAACCAACAAATAATAGT+TGG + chr1_2:8119215-8119234 Msa0053640:three_prime_UTR 35.0%
!! TCTTTTCTTTGGAAAACCGA+TGG + chr1_2:8119251-8119270 Msa0053640:three_prime_UTR 35.0%
GATGATTATGGAGCTTGAAA+CGG + chr1_2:8117212-8117231 Msa0053640:exon 35.0%
!! GTTGATGATGATGATGATCA+AGG + chr1_2:8117294-8117313 Msa0053640:CDS 35.0%
! TGATGATGATGATGATGATG+AGG + chr1_2:8117362-8117381 Msa0053640:CDS 35.0%
GCTGAGAGTAAATATAATGG+TGG + chr1_2:8117426-8117445 Msa0053640:CDS 35.0%
TAGAAGAGGTTCTTCCTATA+AGG + chr1_2:8117472-8117491 Msa0053640:CDS 35.0%
! TTAGTTAGGGTACTAGTTGT+TGG + chr1_2:8117777-8117796 Msa0053640:intron 35.0%
! TGATGGATGATGTGATCTAT+AGG + chr1_2:8117942-8117961 Msa0053640:intron 35.0%
AACATGTTTGCAATTGGTAC+TGG + chr1_2:8118163-8118182 Msa0053640:intron 35.0%
!!! GCCTTTTTTGGTTTTGGTTA+AGG + chr1_2:8118226-8118245 Msa0053640:intron 35.0%
CCTGCATAACCATAAAAGAT+TGG - chr1_2:8118501-8118520 None:intergenic 35.0%
! CCAATCTTTTATGGTTATGC+AGG + chr1_2:8118498-8118517 Msa0053640:intron 35.0%
AAGAAGACGTAGAAATTTGC+TGG + chr1_2:8118631-8118650 Msa0053640:CDS 35.0%
!! AATTTGCTGGCTTTCAATCA+TGG + chr1_2:8118644-8118663 Msa0053640:CDS 35.0%
AAAGAAGACACTCTATTTCC+CGG - chr1_2:8118869-8118888 None:intergenic 35.0%
GAAAAGAAATTCCTCTGCAA+AGG - chr1_2:8118899-8118918 None:intergenic 35.0%
! CATTTTCATTGTTGCAGCAA+TGG - chr1_2:8118945-8118964 None:intergenic 35.0%
! ATTTTCTTTCAGCGAGAAAG+AGG + chr1_2:8119080-8119099 Msa0053640:three_prime_UTR 35.0%
!! AGTTGATGATATATACGGGT+CGG + chr1_2:8119163-8119182 Msa0053640:three_prime_UTR 35.0%
CCGAACCAACAAATAATAGT+TGG + chr1_2:8119215-8119234 Msa0053640:three_prime_UTR 35.0%
!! TCTTTTCTTTGGAAAACCGA+TGG + chr1_2:8119251-8119270 Msa0053640:three_prime_UTR 35.0%
TGTATTATTTGACGCGCGAA+GGG + chr1_2:8117266-8117285 Msa0053640:CDS 40.0%
! TGATGATGATGATCAAGGGA+AGG + chr1_2:8117299-8117318 Msa0053640:CDS 40.0%
GGTTTCATCAGAGAGATCAA+TGG + chr1_2:8117383-8117402 Msa0053640:CDS 40.0%
GAGAGATCAATGGATGAGAA+TGG + chr1_2:8117393-8117412 Msa0053640:CDS 40.0%
! TGGTGGTGCTTTAGATTGTA+TGG + chr1_2:8117443-8117462 Msa0053640:CDS 40.0%
! TGGTGCTTTAGATTGTATGG+AGG + chr1_2:8117446-8117465 Msa0053640:CDS 40.0%
!! TTGTATGGAGGCTTTAGAAG+AGG + chr1_2:8117458-8117477 Msa0053640:CDS 40.0%
ACTCACAGCGATTACAAACA+AGG - chr1_2:8118274-8118293 None:intergenic 40.0%
AAGCTACGAAGTGTAGTTTC+TGG + chr1_2:8118319-8118338 Msa0053640:intron 40.0%
CAATTTGGATCGAGTGAGTT+TGG - chr1_2:8118363-8118382 None:intergenic 40.0%
AACATCAGCTAGGCTTGTAA+AGG - chr1_2:8118561-8118580 None:intergenic 40.0%
CTTCTTGTGTATGCATTCTC+TGG - chr1_2:8118617-8118636 None:intergenic 40.0%
! TTCCCTTTGAGAAGCAATAG+TGG + chr1_2:8118686-8118705 Msa0053640:CDS 40.0%
CACCACTATTGCTTCTCAAA+GGG - chr1_2:8118691-8118710 None:intergenic 40.0%
!! AGAAGGTGCTGAAGAATATG+AGG - chr1_2:8118825-8118844 None:intergenic 40.0%
! GCAGAGGAATTTCTTTTCCT+TGG + chr1_2:8118901-8118920 Msa0053640:CDS 40.0%
TTGGAAAACCGATGGAAAGA+TGG + chr1_2:8119259-8119278 Msa0053640:three_prime_UTR 40.0%
AAACCGATGGAAAGATGGTT+TGG + chr1_2:8119264-8119283 Msa0053640:three_prime_UTR 40.0%
TGTATTATTTGACGCGCGAA+GGG + chr1_2:8117266-8117285 Msa0053640:CDS 40.0%
! TGATGATGATGATCAAGGGA+AGG + chr1_2:8117299-8117318 Msa0053640:CDS 40.0%
GGTTTCATCAGAGAGATCAA+TGG + chr1_2:8117383-8117402 Msa0053640:CDS 40.0%
GAGAGATCAATGGATGAGAA+TGG + chr1_2:8117393-8117412 Msa0053640:CDS 40.0%
! TGGTGGTGCTTTAGATTGTA+TGG + chr1_2:8117443-8117462 Msa0053640:CDS 40.0%
! TGGTGCTTTAGATTGTATGG+AGG + chr1_2:8117446-8117465 Msa0053640:CDS 40.0%
!! TTGTATGGAGGCTTTAGAAG+AGG + chr1_2:8117458-8117477 Msa0053640:CDS 40.0%
ACTCACAGCGATTACAAACA+AGG - chr1_2:8118274-8118293 None:intergenic 40.0%
AAGCTACGAAGTGTAGTTTC+TGG + chr1_2:8118319-8118338 Msa0053640:intron 40.0%
CAATTTGGATCGAGTGAGTT+TGG - chr1_2:8118363-8118382 None:intergenic 40.0%
AACATCAGCTAGGCTTGTAA+AGG - chr1_2:8118561-8118580 None:intergenic 40.0%
CTTCTTGTGTATGCATTCTC+TGG - chr1_2:8118617-8118636 None:intergenic 40.0%
! TTCCCTTTGAGAAGCAATAG+TGG + chr1_2:8118686-8118705 Msa0053640:CDS 40.0%
CACCACTATTGCTTCTCAAA+GGG - chr1_2:8118691-8118710 None:intergenic 40.0%
!! AGAAGGTGCTGAAGAATATG+AGG - chr1_2:8118825-8118844 None:intergenic 40.0%
! GCAGAGGAATTTCTTTTCCT+TGG + chr1_2:8118901-8118920 Msa0053640:CDS 40.0%
TTGGAAAACCGATGGAAAGA+TGG + chr1_2:8119259-8119278 Msa0053640:three_prime_UTR 40.0%
AAACCGATGGAAAGATGGTT+TGG + chr1_2:8119264-8119283 Msa0053640:three_prime_UTR 40.0%
GATTATGGAGCTTGAAACGG+TGG + chr1_2:8117215-8117234 Msa0053640:CDS 45.0%
CTGTATTATTTGACGCGCGA+AGG + chr1_2:8117265-8117284 Msa0053640:CDS 45.0%
GTCTATCCAGTAGTGTGTGA+TGG + chr1_2:8117925-8117944 Msa0053640:intron 45.0%
CATCATCCATCACACACTAC+TGG - chr1_2:8117934-8117953 None:intergenic 45.0%
! CTACGAAGTGTAGTTTCTGG+TGG + chr1_2:8118322-8118341 Msa0053640:intron 45.0%
GTGTTGTGACAACATCAGCT+AGG - chr1_2:8118571-8118590 None:intergenic 45.0%
!! TGCTGGCTTTCAATCATGGT+TGG + chr1_2:8118648-8118667 Msa0053640:CDS 45.0%
!! GCTGGCTTTCAATCATGGTT+GGG + chr1_2:8118649-8118668 Msa0053640:CDS 45.0%
CCACCACTATTGCTTCTCAA+AGG - chr1_2:8118692-8118711 None:intergenic 45.0%
! CCTTTGAGAAGCAATAGTGG+TGG + chr1_2:8118689-8118708 Msa0053640:CDS 45.0%
CTTTAGCATCTCCTTTGCAG+AGG + chr1_2:8118885-8118904 Msa0053640:CDS 45.0%
ACAATGATGCAGATCAGCCA+AGG - chr1_2:8118921-8118940 None:intergenic 45.0%
GATTATGGAGCTTGAAACGG+TGG + chr1_2:8117215-8117234 Msa0053640:CDS 45.0%
CTGTATTATTTGACGCGCGA+AGG + chr1_2:8117265-8117284 Msa0053640:CDS 45.0%
GTCTATCCAGTAGTGTGTGA+TGG + chr1_2:8117925-8117944 Msa0053640:intron 45.0%
CATCATCCATCACACACTAC+TGG - chr1_2:8117934-8117953 None:intergenic 45.0%
! CTACGAAGTGTAGTTTCTGG+TGG + chr1_2:8118322-8118341 Msa0053640:intron 45.0%
GTGTTGTGACAACATCAGCT+AGG - chr1_2:8118571-8118590 None:intergenic 45.0%
!! TGCTGGCTTTCAATCATGGT+TGG + chr1_2:8118648-8118667 Msa0053640:CDS 45.0%
!! GCTGGCTTTCAATCATGGTT+GGG + chr1_2:8118649-8118668 Msa0053640:CDS 45.0%
CCACCACTATTGCTTCTCAA+AGG - chr1_2:8118692-8118711 None:intergenic 45.0%
! CCTTTGAGAAGCAATAGTGG+TGG + chr1_2:8118689-8118708 Msa0053640:CDS 45.0%
CTTTAGCATCTCCTTTGCAG+AGG + chr1_2:8118885-8118904 Msa0053640:CDS 45.0%
ACAATGATGCAGATCAGCCA+AGG - chr1_2:8118921-8118940 None:intergenic 45.0%
!! AAAATACAATATAATATAAT+AGG - chr1_2:8117521-8117540 None:intergenic 5.0%
!!! TAATTTTTTGTAAATTATAA+AGG + chr1_2:8118470-8118489 Msa0053640:intron 5.0%
!! AAAATACAATATAATATAAT+AGG - chr1_2:8117521-8117540 None:intergenic 5.0%
!!! TAATTTTTTGTAAATTATAA+AGG + chr1_2:8118470-8118489 Msa0053640:intron 5.0%
ACGACGTCGTCTTCATCGAT+CGG + chr1_2:8117330-8117349 Msa0053640:CDS 50.0%
ACTCTATTTCCCGGATGACG+CGG - chr1_2:8118860-8118879 None:intergenic 50.0%
ACGACGTCGTCTTCATCGAT+CGG + chr1_2:8117330-8117349 Msa0053640:CDS 50.0%
ACTCTATTTCCCGGATGACG+CGG - chr1_2:8118860-8118879 None:intergenic 50.0%
AACGGTGGCGTTTAAGCGCG+TGG + chr1_2:8117230-8117249 Msa0053640:CDS 60.0%
ACGGTGGCGTTTAAGCGCGT+GGG + chr1_2:8117231-8117250 Msa0053640:CDS 60.0%
CTATTTCCCGGATGACGCGG+TGG - chr1_2:8118857-8118876 None:intergenic 60.0%
AACGGTGGCGTTTAAGCGCG+TGG + chr1_2:8117230-8117249 Msa0053640:CDS 60.0%
ACGGTGGCGTTTAAGCGCGT+GGG + chr1_2:8117231-8117250 Msa0053640:CDS 60.0%
CTATTTCCCGGATGACGCGG+TGG - chr1_2:8118857-8118876 None:intergenic 60.0%
CGCGGTGGAAGTGGCGAAGA+AGG - chr1_2:8118842-8118861 None:intergenic 65.0%
GCCACTTCCACCGCGTCATC+CGG + chr1_2:8118847-8118866 Msa0053640:CDS 65.0%
CCACTTCCACCGCGTCATCC+GGG + chr1_2:8118848-8118867 Msa0053640:CDS 65.0%
CGCGGTGGAAGTGGCGAAGA+AGG - chr1_2:8118842-8118861 None:intergenic 65.0%
GCCACTTCCACCGCGTCATC+CGG + chr1_2:8118847-8118866 Msa0053640:CDS 65.0%
CCACTTCCACCGCGTCATCC+GGG + chr1_2:8118848-8118867 Msa0053640:CDS 65.0%
CCCGGATGACGCGGTGGAAG+TGG - chr1_2:8118851-8118870 None:intergenic 70.0%
CCCGGATGACGCGGTGGAAG+TGG - chr1_2:8118851-8118870 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 8117126 8119287 8117126 ID=Msa0053640;Name=Msa0053640
chr1_2 mRNA 8117126 8119287 8117126 ID=Msa0053640-mRNA-1;Parent=Msa0053640;Name=Msa0053640-mRNA-1;_AED=0.01;_eAED=0.01;_QI=87|1|1|1|1|1|2|254|264
chr1_2 exon 8117126 8117493 8117126 ID=Msa0053640-mRNA-1:exon:2842;Parent=Msa0053640-mRNA-1
chr1_2 exon 8118520 8119287 8118520 ID=Msa0053640-mRNA-1:exon:2843;Parent=Msa0053640-mRNA-1
chr1_2 five_prime_UTR 8117126 8117212 8117126 ID=Msa0053640-mRNA-1:five_prime_utr;Parent=Msa0053640-mRNA-1
chr1_2 CDS 8117213 8117493 8117213 ID=Msa0053640-mRNA-1:cds;Parent=Msa0053640-mRNA-1
chr1_2 CDS 8118520 8119033 8118520 ID=Msa0053640-mRNA-1:cds;Parent=Msa0053640-mRNA-1
chr1_2 three_prime_UTR 8119034 8119287 8119034 ID=Msa0053640-mRNA-1:three_prime_utr;Parent=Msa0053640-mRNA-1
Gene Sequence

>Msa0053640

ATGATTATGGAGCTTGAAACGGTGGCGTTTAAGCGCGTGGGATCACGTACTTCTGTATTATTTGACGCGCGAAGGGATCATGTTGATGATGATGATGATCAAGGGAAGGTGTGTAGTACGACGTCGTCTTCATCGATCGGAAGAAATAGTGATGATGATGATGATGATGAGGTTTCATCAGAGAGATCAATGGATGAGAATGGTGAAAATGAAGCTGAGAGTAAATATAATGGTGGTGCTTTAGATTGTATGGAGGCTTTAGAAGAGGTTCTTCCTATAAGGAGAAGTATCTCAAATTTCTATAGCGGGAAGTCTAAGTCCTTTACAAGCCTAGCTGATGTTGTCACAACACCATCTGTAAAAGACATTGTAAAACCAGAGAATGCATACACAAGAAGACGTAGAAATTTGCTGGCTTTCAATCATGGTTGGGACAAGAACAAAAATTTCCCTTTGAGAAGCAATAGTGGTGGAATTTCAAAGAGAACAATGAGCTTAAGTAGAAGTGCTTTAGCTCTTGCAGTTGCATTAAGCAATTCTGATAGTAGCTCTAGTTTTACAAGTGATGATTCAGCTGCTTCAACCTCATATTCTTCAGCACCTTCTTCGCCACTTCCACCGCGTCATCCGGGAAATAGAGTGTCTTCTTTAGCATCTCCTTTGCAGAGGAATTTCTTTTCCTTGGCTGATCTGCATCATTGTGCCATTGCTGCAACAATGAAAATGCCAAATTCTTCAATTGAAAATGAAACAACTAATCATCCATCACAGTGTATAAAACCTTATGATAACTAA
Protein sequence

>Msa0053640

MIMELETVAFKRVGSRTSVLFDARRDHVDDDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENGENEAESKYNGGALDCMEALEEVLPIRRSISNFYSGKSKSFTSLADVVTTPSVKDIVKPENAYTRRRRNLLAFNHGWDKNKNFPLRSNSGGISKRTMSLSRSALALAVALSNSDSSSSFTSDDSAASTSYSSAPSSPLPPRHPGNRVSSLASPLQRNFFSLADLHHCAIAATMKMPNSSIENETTNHPSQCIKPYDN*