AlfalfaGEDB | Msa0175640

M. sativa cultivar zhongmu-4 / Msa0175640

KEGG: K03676
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0006995 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044424 GO:0044464 GO:0050896 GO:0051716 GO:0071496
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175640	sp\|Q9M9Y9\|GRS11_ARATH	75.258	97	24	0	1	97	1	97	1.53e-51	160

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175640	A0A072VN37	98.990	99	1	0	1	99	1	99	2.76e-65	201

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0082540	Msa0175640	0.911250	7.523267e-83	-8.615850e-47
Msa0082550	Msa0175640	0.856719	2.659925e-62	-8.615850e-47
Msa0138230	Msa0175640	0.948777	4.808091e-107	-8.615850e-47
Msa0138240	Msa0175640	0.863230	2.887134e-64	-8.615850e-47
Msa0175630	Msa0175640	0.870112	1.865855e-66	-8.615850e-47
Msa0175640	Msa0282600	0.814531	1.468980e-51	-8.615850e-47
Msa0175640	Msa0416800	0.835681	1.456895e-56	-8.615850e-47
Msa0175640	Msa0463460	0.835675	1.461349e-56	-8.615850e-47
Msa0175640	Msa0509020	0.830629	2.636639e-55	-8.615850e-47
Msa0175640	Msa0722930	0.806338	8.634779e-50	-8.615850e-47
Msa0175640	Msa0723140	0.806370	8.502591e-50	-8.615850e-47
Msa0175640	Msa0744500	0.801296	9.625381e-49	-8.615850e-47
Msa0175640	Msa0790230	0.870844	1.073585e-66	-8.615850e-47
Msa0175640	Msa0790240	0.953364	3.225178e-111	-8.615850e-47
Msa0175640	Msa0826650	0.801011	1.101306e-48	-8.615850e-47
Msa0175640	Msa0864690	0.800074	1.709583e-48	-8.615850e-47
Msa0175640	Msa1055430	0.804628	1.971498e-49	-8.615850e-47
Msa0175640	Msa1249940	0.804090	2.552062e-49	-8.615850e-47
Msa0175640	Msa1400460	0.949082	2.609913e-107	-8.615850e-47
Msa0175640	Msa1400470	0.859899	3.004706e-63	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175640	MtrunA17_Chr1g0195981	98.990	99	1	0	1	99	1	99	5.32e-69	201
Msa0175640	MtrunA17_Chr1g0195991	95.960	99	4	0	1	99	1	99	4.05e-68	199
Msa0175640	MtrunA17_Chr7g0268711	79.592	98	20	0	1	98	1	98	3.18e-58	174
Msa0175640	MtrunA17_Chr2g0286251	58.947	95	39	0	1	95	1	95	2.11e-39	126
Msa0175640	MtrunA17_Chr1g0195971	55.102	98	44	0	1	98	1	98	4.78e-37	120
Msa0175640	MtrunA17_Chr2g0286271	55.789	95	42	0	1	95	1	95	1.98e-36	119
Msa0175640	MtrunA17_Chr7g0222541	54.167	96	43	1	1	95	30	125	6.06e-35	116
Msa0175640	MtrunA17_Chr7g0222551	53.125	96	44	1	1	95	30	125	1.91e-34	115
Msa0175640	MtrunA17_Chr7g0224091	54.167	96	43	1	1	95	22	117	3.71e-34	114
Msa0175640	MtrunA17_Chr7g0222491	53.125	96	44	1	1	95	30	125	1.78e-33	112
Msa0175640	MtrunA17_Chr4g0066791	52.083	96	45	1	1	95	1	96	1.64e-32	109
Msa0175640	MtrunA17_Chr4g0066801	52.083	96	45	1	1	95	1	96	1.64e-32	109
Msa0175640	MtrunA17_Chr4g0066811	52.083	96	45	1	1	95	1	96	1.64e-32	109
Msa0175640	MtrunA17_Chr7g0268731	52.632	95	44	1	1	94	1	95	1.28e-31	107
Msa0175640	MtrunA17_Chr7g0268721	51.579	95	45	1	1	94	1	95	8.81e-31	104
Msa0175640	MtrunA17_Chr7g0268751	43.158	95	54	0	1	95	1	95	9.16e-26	92.0
Msa0175640	MtrunA17_Chr1g0195951	40.426	94	56	0	1	94	1	94	3.15e-25	90.9
Msa0175640	MtrunA17_Chr1g0195931	44.898	98	54	0	1	98	1	98	1.65e-21	81.6
Msa0175640	MtrunA17_Chr2g0303741	41.837	98	53	1	2	95	40	137	5.06e-21	81.3
Msa0175640	MtrunA17_Chr2g0282951	40.426	94	56	0	2	95	27	120	4.29e-20	78.6
Msa0175640	MtrunA17_Chr7g0268761	41.489	94	55	0	1	94	1	94	4.78e-20	77.8
Msa0175640	MtrunA17_Chr2g0325001	36.458	96	54	2	4	95	41	133	1.52e-18	74.7
Msa0175640	MtrunA17_Chr3g0117411	35.789	95	61	0	1	95	18	112	2.58e-16	68.6
Msa0175640	MtrunA17_Chr4g0040601	38.710	93	52	3	6	95	58	148	7.40e-16	68.2
Msa0175640	MtrunA17_Chr3g0117391	34.043	94	62	0	1	94	21	114	1.06e-15	67.0
Msa0175640	MtrunA17_Chr7g0228311	35.106	94	61	0	1	94	3	96	6.50e-15	64.7
Msa0175640	MtrunA17_Chr1g0151231	38.298	94	56	1	3	94	48	141	1.74e-14	64.7
Msa0175640	MtrunA17_Chr1g0182971	32.979	94	62	1	2	94	84	177	5.08e-14	64.3
Msa0175640	MtrunA17_Chr3g0135811	37.634	93	52	3	3	94	23	110	3.05e-13	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175640	AT1G06830.1	75.258	97	24	0	1	97	1	97	1.56e-52	160
Msa0175640	AT2G30540.1	70.408	98	29	0	1	98	1	98	4.21e-52	159
Msa0175640	AT2G47880.1	72.449	98	27	0	1	98	1	98	5.41e-52	158
Msa0175640	AT3G62960.1	70.408	98	29	0	1	98	1	98	2.11e-50	154
Msa0175640	AT3G62950.1	51.042	96	46	1	1	95	1	96	2.22e-36	119
Msa0175640	AT2G47870.1	53.125	96	44	1	1	95	1	96	9.73e-36	117
Msa0175640	AT4G15670.1	50.526	95	47	0	1	95	1	95	3.39e-34	114
Msa0175640	AT3G21460.1	54.737	95	43	0	1	95	1	95	4.27e-34	113
Msa0175640	AT4G15680.1	51.579	95	46	0	1	95	1	95	1.16e-33	112
Msa0175640	AT4G15660.1	49.485	97	49	0	1	97	1	97	1.17e-33	112
Msa0175640	AT4G15700.1	51.579	95	46	0	1	95	1	95	1.30e-33	112
Msa0175640	AT4G15690.1	50.526	95	47	0	1	95	1	95	1.14e-32	110
Msa0175640	AT5G18600.1	48.421	95	49	0	1	95	1	95	1.63e-31	107
Msa0175640	AT5G14070.1	45.631	103	48	2	1	95	31	133	1.31e-26	95.9
Msa0175640	AT3G02000.1	45.455	99	48	1	3	95	31	129	2.60e-25	92.4
Msa0175640	AT3G62930.1	43.158	95	54	0	1	95	1	95	2.93e-25	91.3
Msa0175640	AT1G03020.1	45.263	95	52	0	1	95	1	95	7.03e-25	90.1
Msa0175640	AT4G33040.1	42.268	97	50	2	3	94	41	136	1.61e-17	72.8
Msa0175640	AT1G28480.1	36.735	98	58	2	2	95	33	130	2.62e-17	72.0
Msa0175640	AT5G40370.1	32.979	94	63	0	1	94	3	96	1.01e-14	64.7
Msa0175640	AT5G11930.1	41.935	93	44	3	3	94	57	140	1.55e-14	65.1
Msa0175640	AT4G28730.1	35.106	94	60	1	2	94	71	164	1.66e-14	65.5
Msa0175640	AT5G63030.1	36.585	82	52	0	13	94	31	112	2.68e-12	58.9
Msa0175640	AT2G20270.1	34.043	94	61	1	2	94	76	169	6.84e-12	58.9
Msa0175640	AT5G20500.1	31.868	91	62	0	4	94	35	125	1.49e-11	57.0
Msa0175640	AT4G28730.2	37.143	70	43	1	2	70	71	140	8.55e-11	55.1
Msa0175640	AT1G03850.2	34.884	86	52	2	10	95	62	143	9.75e-11	55.1

Find 33 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATTCTGTTTCAAGAAAT+TGG	0.120000	1_4:-61644531	Msa0175640:CDS
AGATCCTGAAGGAAAAGAAA+TGG	0.394593	1_4:-61644478	Msa0175640:CDS
GTGGAGGGACATTACTTCAT+TGG	0.411453	1_4:+61644380	None:intergenic
GGAGAAAGCTATAACAAAGT+TGG	0.418322	1_4:-61644457	Msa0175640:CDS
CCTGTCCCTGCAGTATTCAT+TGG	0.431206	1_4:-61644423	Msa0175640:CDS
GAGAAAGCTATAACAAAGTT+GGG	0.448427	1_4:-61644456	Msa0175640:CDS
GATGAGGTTGGCTACAGAAA+AGG	0.457049	1_4:-61644601	Msa0175640:CDS
TTCATTGGAGGAAAGCTTGT+GGG	0.469806	1_4:-61644408	Msa0175640:CDS
TTTCCTCCAATGAATACTGC+AGG	0.495376	1_4:+61644417	None:intergenic
TTGTCAATTTCATGAACCAC+AGG	0.499275	1_4:+61644501	None:intergenic
TGATCAAATTAAGCTAAATA+CGG	0.503808	1_4:+61644322	None:intergenic
ATGAGGTTGGCTACAGAAAA+GGG	0.514459	1_4:-61644600	Msa0175640:CDS
GATGGAGAAGGTGATGAGGT+TGG	0.515048	1_4:-61644613	Msa0175640:CDS
GCACAAACAACAAGAGCTCT+TGG	0.517591	1_4:+61644563	None:intergenic
CCAATGAATACTGCAGGGAC+AGG	0.522380	1_4:+61644423	None:intergenic
GAGGGACATTACTTCATTGG+TGG	0.543110	1_4:+61644383	None:intergenic
GTCCCTGCAGTATTCATTGG+AGG	0.543693	1_4:-61644420	Msa0175640:CDS
AAATTAAGCTAAATACGGTA+TGG	0.562707	1_4:+61644327	None:intergenic
CGAGTGAACCACTTAGGTGG+AGG	0.574745	1_4:+61644364	None:intergenic
ATACGGTATGGCCTAAGTAG+TGG	0.575235	1_4:+61644339	None:intergenic
GTAATGTCCCTCCACCTAAG+TGG	0.591093	1_4:-61644372	Msa0175640:CDS
GTTCACTCGTTCCACTACTT+AGG	0.591318	1_4:-61644350	Msa0175640:CDS
GTGGAACGAGTGAACCACTT+AGG	0.595515	1_4:+61644358	None:intergenic
CAGTTATATTAAGATGGAGA+AGG	0.604195	1_4:-61644625	None:intergenic
TCATTGGAGGAAAGCTTGTG+GGG	0.613505	1_4:-61644407	Msa0175640:CDS
GAAATTGACAAAGATCCTGA+AGG	0.616874	1_4:-61644489	Msa0175640:CDS
ATTCATTGGAGGAAAGCTTG+TGG	0.621609	1_4:-61644409	Msa0175640:CDS
GAACGAGTGAACCACTTAGG+TGG	0.634239	1_4:+61644361	None:intergenic
TTCCTCCAATGAATACTGCA+GGG	0.637919	1_4:+61644418	None:intergenic
GAGTGAACCACTTAGGTGGA+GGG	0.652910	1_4:+61644365	None:intergenic
TTAAGATGGAGAAGGTGATG+AGG	0.668317	1_4:-61644617	None:intergenic
TGTCAATTTCATGAACCACA+GGG	0.678104	1_4:+61644502	None:intergenic
AGAAATTGGAGTGTTCCCTG+TGG	0.708961	1_4:-61644517	Msa0175640:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACATTCTGTTTCAAGAAAT+TGG	-	chr1_4:61644416-61644435	Msa0175640:CDS	25.0%
	GAGAAAGCTATAACAAAGTT+GGG	-	chr1_4:61644491-61644510	Msa0175640:CDS	30.0%
	TGTCAATTTCATGAACCACA+GGG	+	chr1_4:61644448-61644467	None:intergenic	35.0%
	TTGTCAATTTCATGAACCAC+AGG	+	chr1_4:61644449-61644468	None:intergenic	35.0%
	GAAATTGACAAAGATCCTGA+AGG	-	chr1_4:61644458-61644477	Msa0175640:CDS	35.0%
	AGATCCTGAAGGAAAAGAAA+TGG	-	chr1_4:61644469-61644488	Msa0175640:CDS	35.0%
!	TTCTCCATTTCTTTTCCTTC+AGG	+	chr1_4:61644476-61644495	None:intergenic	35.0%
	GGAGAAAGCTATAACAAAGT+TGG	-	chr1_4:61644490-61644509	Msa0175640:CDS	35.0%
!	ATGAGGTTGGCTACAGAAAA+GGG	-	chr1_4:61644347-61644366	Msa0175640:CDS	40.0%
	TTCCTCCAATGAATACTGCA+GGG	+	chr1_4:61644532-61644551	None:intergenic	40.0%
	TTTCCTCCAATGAATACTGC+AGG	+	chr1_4:61644533-61644552	None:intergenic	40.0%
	ATTCATTGGAGGAAAGCTTG+TGG	-	chr1_4:61644538-61644557	Msa0175640:CDS	40.0%
	TTCATTGGAGGAAAGCTTGT+GGG	-	chr1_4:61644539-61644558	Msa0175640:CDS	40.0%
!	GATGAGGTTGGCTACAGAAA+AGG	-	chr1_4:61644346-61644365	Msa0175640:CDS	45.0%
	GCACAAACAACAAGAGCTCT+TGG	+	chr1_4:61644387-61644406	None:intergenic	45.0%
!	AGAAATTGGAGTGTTCCCTG+TGG	-	chr1_4:61644430-61644449	Msa0175640:CDS	45.0%
	TCATTGGAGGAAAGCTTGTG+GGG	-	chr1_4:61644540-61644559	Msa0175640:CDS	45.0%
	GAGGGACATTACTTCATTGG+TGG	+	chr1_4:61644567-61644586	None:intergenic	45.0%
	GTGGAGGGACATTACTTCAT+TGG	+	chr1_4:61644570-61644589	None:intergenic	45.0%
	GTTCACTCGTTCCACTACTT+AGG	-	chr1_4:61644597-61644616	Msa0175640:CDS	45.0%
	ATACGGTATGGCCTAAGTAG+TGG	+	chr1_4:61644611-61644630	None:intergenic	45.0%
	CCAATGAATACTGCAGGGAC+AGG	+	chr1_4:61644527-61644546	None:intergenic	50.0%
	CCTGTCCCTGCAGTATTCAT+TGG	-	chr1_4:61644524-61644543	Msa0175640:CDS	50.0%
	GTCCCTGCAGTATTCATTGG+AGG	-	chr1_4:61644527-61644546	Msa0175640:CDS	50.0%
	GTAATGTCCCTCCACCTAAG+TGG	-	chr1_4:61644575-61644594	Msa0175640:CDS	50.0%
	GAGTGAACCACTTAGGTGGA+GGG	+	chr1_4:61644585-61644604	None:intergenic	50.0%
	GAACGAGTGAACCACTTAGG+TGG	+	chr1_4:61644589-61644608	None:intergenic	50.0%
	GTGGAACGAGTGAACCACTT+AGG	+	chr1_4:61644592-61644611	None:intergenic	50.0%
	CGAGTGAACCACTTAGGTGG+AGG	+	chr1_4:61644586-61644605	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	61644335	61644634	61644335	ID=Msa0175640;Name=Msa0175640
chr1_4	mRNA	61644335	61644634	61644335	ID=Msa0175640-mRNA-1;Parent=Msa0175640;Name=Msa0175640-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|99
chr1_4	exon	61644335	61644634	61644335	ID=Msa0175640-mRNA-1:exon:19722;Parent=Msa0175640-mRNA-1
chr1_4	CDS	61644335	61644634	61644335	ID=Msa0175640-mRNA-1:cds;Parent=Msa0175640-mRNA-1

Gene Sequence	>Msa0175640 ATGGAGAAGGTGATGAGGTTGGCTACAGAAAAGGGTGTTGTGATATTCACCAAGAGCTCTTGTTGTTTGTGCTATGCAGTGAACATTCTGTTTCAAGAAATTGGAGTGTTCCCTGTGGTTCATGAAATTGACAAAGATCCTGAAGGAAAAGAAATGGAGAAAGCTATAACAAAGTTGGGTTGTAATGCACCTGTCCCTGCAGTATTCATTGGAGGAAAGCTTGTGGGGTCCACCAATGAAGTAATGTCCCTCCACCTAAGTGGTTCACTCGTTCCACTACTTAGGCCATACCGTATTTAG
Protein sequence	>Msa0175640 MEKVMRLATEKGVVIFTKSSCCLCYAVNILFQEIGVFPVVHEIDKDPEGKEMEKAITKLGCNAPVPAVFIGGKLVGSTNEVMSLHLSGSLVPLLRPYRI*