AlfalfaGEDB | Msa0138230

M. sativa cultivar zhongmu-4 / Msa0138230

KEGG: K03676
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0006995 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044424 GO:0044464 GO:0050896 GO:0051716 GO:0071496
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138230	sp\|Q9M9Y9\|GRS11_ARATH	75.258	97	24	0	1	97	1	97	1.53e-51	160

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138230	A0A072VN37	98.990	99	1	0	1	99	1	99	2.76e-65	201

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0082540	Msa0138230	0.957114	5.912028e-115	-8.615850e-47
Msa0082550	Msa0138230	0.889444	2.408772e-73	-8.615850e-47
Msa0124110	Msa0138230	0.809555	1.785695e-50	-8.615850e-47
Msa0138230	Msa0138240	0.894876	1.632062e-75	-8.615850e-47
Msa0138230	Msa0175630	0.893978	3.797642e-75	-8.615850e-47
Msa0138230	Msa0175640	0.948777	4.808091e-107	-8.615850e-47
Msa0138230	Msa0282600	0.813282	2.769717e-51	-8.615850e-47
Msa0138230	Msa0304990	0.804724	1.882914e-49	-8.615850e-47
Msa0138230	Msa0416800	0.840430	8.735557e-58	-8.615850e-47
Msa0138230	Msa0463460	0.834928	2.256256e-56	-8.615850e-47
Msa0138230	Msa0509020	0.829220	5.815573e-55	-8.615850e-47
Msa0138230	Msa0722930	0.815882	7.362922e-52	-8.615850e-47
Msa0138230	Msa0723140	0.817289	3.561977e-52	-8.615850e-47
Msa0138230	Msa0790230	0.897602	1.199702e-76	-8.615850e-47
Msa0138230	Msa0790240	0.986830	4.072146e-168	-8.615850e-47
Msa0138230	Msa0843280	0.807343	5.294901e-50	-8.615850e-47
Msa0138230	Msa1036390	0.800449	1.433878e-48	-8.615850e-47
Msa0138230	Msa1055430	0.811376	7.221625e-51	-8.615850e-47
Msa0138230	Msa1139240	0.801554	8.523855e-49	-8.615850e-47
Msa0138230	Msa1249940	0.820648	6.139812e-53	-8.615850e-47
Msa0138230	Msa1400460	0.993871	7.686890e-203	-8.615850e-47
Msa0138230	Msa1400470	0.892148	2.072715e-74	-8.615850e-47
Msa0138230	Msa1410180	0.802690	4.977602e-49	-8.615850e-47
Msa0138230	Msa1444060	-0.803259	3.797147e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138230	MtrunA17_Chr1g0195981	98.990	99	1	0	1	99	1	99	5.32e-69	201
Msa0138230	MtrunA17_Chr1g0195991	95.960	99	4	0	1	99	1	99	4.05e-68	199
Msa0138230	MtrunA17_Chr7g0268711	79.592	98	20	0	1	98	1	98	3.18e-58	174
Msa0138230	MtrunA17_Chr2g0286251	58.947	95	39	0	1	95	1	95	2.11e-39	126
Msa0138230	MtrunA17_Chr1g0195971	55.102	98	44	0	1	98	1	98	4.78e-37	120
Msa0138230	MtrunA17_Chr2g0286271	55.789	95	42	0	1	95	1	95	1.98e-36	119
Msa0138230	MtrunA17_Chr7g0222541	54.167	96	43	1	1	95	30	125	6.06e-35	116
Msa0138230	MtrunA17_Chr7g0222551	53.125	96	44	1	1	95	30	125	1.91e-34	115
Msa0138230	MtrunA17_Chr7g0224091	54.167	96	43	1	1	95	22	117	3.71e-34	114
Msa0138230	MtrunA17_Chr7g0222491	53.125	96	44	1	1	95	30	125	1.78e-33	112
Msa0138230	MtrunA17_Chr4g0066791	52.083	96	45	1	1	95	1	96	1.64e-32	109
Msa0138230	MtrunA17_Chr4g0066801	52.083	96	45	1	1	95	1	96	1.64e-32	109
Msa0138230	MtrunA17_Chr4g0066811	52.083	96	45	1	1	95	1	96	1.64e-32	109
Msa0138230	MtrunA17_Chr7g0268731	52.632	95	44	1	1	94	1	95	1.28e-31	107
Msa0138230	MtrunA17_Chr7g0268721	51.579	95	45	1	1	94	1	95	8.81e-31	104
Msa0138230	MtrunA17_Chr7g0268751	43.158	95	54	0	1	95	1	95	9.16e-26	92.0
Msa0138230	MtrunA17_Chr1g0195951	40.426	94	56	0	1	94	1	94	3.15e-25	90.9
Msa0138230	MtrunA17_Chr1g0195931	44.898	98	54	0	1	98	1	98	1.65e-21	81.6
Msa0138230	MtrunA17_Chr2g0303741	41.837	98	53	1	2	95	40	137	5.06e-21	81.3
Msa0138230	MtrunA17_Chr2g0282951	40.426	94	56	0	2	95	27	120	4.29e-20	78.6
Msa0138230	MtrunA17_Chr7g0268761	41.489	94	55	0	1	94	1	94	4.78e-20	77.8
Msa0138230	MtrunA17_Chr2g0325001	36.458	96	54	2	4	95	41	133	1.52e-18	74.7
Msa0138230	MtrunA17_Chr3g0117411	35.789	95	61	0	1	95	18	112	2.58e-16	68.6
Msa0138230	MtrunA17_Chr4g0040601	38.710	93	52	3	6	95	58	148	7.40e-16	68.2
Msa0138230	MtrunA17_Chr3g0117391	34.043	94	62	0	1	94	21	114	1.06e-15	67.0
Msa0138230	MtrunA17_Chr7g0228311	35.106	94	61	0	1	94	3	96	6.50e-15	64.7
Msa0138230	MtrunA17_Chr1g0151231	38.298	94	56	1	3	94	48	141	1.74e-14	64.7
Msa0138230	MtrunA17_Chr1g0182971	32.979	94	62	1	2	94	84	177	5.08e-14	64.3
Msa0138230	MtrunA17_Chr3g0135811	37.634	93	52	3	3	94	23	110	3.05e-13	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0138230	AT1G06830.1	75.258	97	24	0	1	97	1	97	1.56e-52	160
Msa0138230	AT2G30540.1	70.408	98	29	0	1	98	1	98	4.21e-52	159
Msa0138230	AT2G47880.1	72.449	98	27	0	1	98	1	98	5.41e-52	158
Msa0138230	AT3G62960.1	70.408	98	29	0	1	98	1	98	2.11e-50	154
Msa0138230	AT3G62950.1	51.042	96	46	1	1	95	1	96	2.22e-36	119
Msa0138230	AT2G47870.1	53.125	96	44	1	1	95	1	96	9.73e-36	117
Msa0138230	AT4G15670.1	50.526	95	47	0	1	95	1	95	3.39e-34	114
Msa0138230	AT3G21460.1	54.737	95	43	0	1	95	1	95	4.27e-34	113
Msa0138230	AT4G15680.1	51.579	95	46	0	1	95	1	95	1.16e-33	112
Msa0138230	AT4G15660.1	49.485	97	49	0	1	97	1	97	1.17e-33	112
Msa0138230	AT4G15700.1	51.579	95	46	0	1	95	1	95	1.30e-33	112
Msa0138230	AT4G15690.1	50.526	95	47	0	1	95	1	95	1.14e-32	110
Msa0138230	AT5G18600.1	48.421	95	49	0	1	95	1	95	1.63e-31	107
Msa0138230	AT5G14070.1	45.631	103	48	2	1	95	31	133	1.31e-26	95.9
Msa0138230	AT3G02000.1	45.455	99	48	1	3	95	31	129	2.60e-25	92.4
Msa0138230	AT3G62930.1	43.158	95	54	0	1	95	1	95	2.93e-25	91.3
Msa0138230	AT1G03020.1	45.263	95	52	0	1	95	1	95	7.03e-25	90.1
Msa0138230	AT4G33040.1	42.268	97	50	2	3	94	41	136	1.61e-17	72.8
Msa0138230	AT1G28480.1	36.735	98	58	2	2	95	33	130	2.62e-17	72.0
Msa0138230	AT5G40370.1	32.979	94	63	0	1	94	3	96	1.01e-14	64.7
Msa0138230	AT5G11930.1	41.935	93	44	3	3	94	57	140	1.55e-14	65.1
Msa0138230	AT4G28730.1	35.106	94	60	1	2	94	71	164	1.66e-14	65.5
Msa0138230	AT5G63030.1	36.585	82	52	0	13	94	31	112	2.68e-12	58.9
Msa0138230	AT2G20270.1	34.043	94	61	1	2	94	76	169	6.84e-12	58.9
Msa0138230	AT5G20500.1	31.868	91	62	0	4	94	35	125	1.49e-11	57.0
Msa0138230	AT4G28730.2	37.143	70	43	1	2	70	71	140	8.55e-11	55.1
Msa0138230	AT1G03850.2	34.884	86	52	2	10	95	62	143	9.75e-11	55.1

Find 33 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATTCTGTTTCAAGAAAT+TGG	0.120000	1_3:+71099074	Msa0138230:CDS
AGATCCTGAAGGAAAAGAAA+TGG	0.394593	1_3:+71099127	Msa0138230:CDS
GTGGAGGGACATTACTTCAT+TGG	0.411453	1_3:-71099225	None:intergenic
GGAGAAAGCTATAACAAAGT+TGG	0.418322	1_3:+71099148	Msa0138230:CDS
CCTGTCCCTGCAGTATTCAT+TGG	0.431206	1_3:+71099182	Msa0138230:CDS
GAGAAAGCTATAACAAAGTT+GGG	0.448427	1_3:+71099149	Msa0138230:CDS
GATGAGGTTGGCTACAGAAA+AGG	0.457049	1_3:+71099004	Msa0138230:CDS
TTCATTGGAGGAAAGCTTGT+GGG	0.469806	1_3:+71099197	Msa0138230:CDS
TTTCCTCCAATGAATACTGC+AGG	0.495376	1_3:-71099188	None:intergenic
TTGTCAATTTCATGAACCAC+AGG	0.499275	1_3:-71099104	None:intergenic
TGATCAAATTAAGCTAAATA+CGG	0.503808	1_3:-71099283	None:intergenic
ATGAGGTTGGCTACAGAAAA+GGG	0.514459	1_3:+71099005	Msa0138230:CDS
GATGGAGAAGGTGATGAGGT+TGG	0.515048	1_3:+71098992	None:intergenic
GCACAAACAACAAGAGCTCT+TGG	0.517591	1_3:-71099042	None:intergenic
CCAATGAATACTGCAGGGAC+AGG	0.522380	1_3:-71099182	None:intergenic
GAGGGACATTACTTCATTGG+TGG	0.543110	1_3:-71099222	None:intergenic
GTCCCTGCAGTATTCATTGG+AGG	0.543693	1_3:+71099185	Msa0138230:CDS
AAATTAAGCTAAATACGGTA+TGG	0.562707	1_3:-71099278	None:intergenic
CGAGTGAACCACTTAGGTGG+AGG	0.574745	1_3:-71099241	None:intergenic
ATACGGTATGGCCTAAGTAG+TGG	0.575235	1_3:-71099266	None:intergenic
GTAATGTCCCTCCACCTAAG+TGG	0.591093	1_3:+71099233	Msa0138230:CDS
GTTCACTCGTTCCACTACTT+AGG	0.591318	1_3:+71099255	Msa0138230:CDS
GTGGAACGAGTGAACCACTT+AGG	0.595515	1_3:-71099247	None:intergenic
CAGTTATATTAAGATGGAGA+AGG	0.604195	1_3:+71098980	None:intergenic
TCATTGGAGGAAAGCTTGTG+GGG	0.613505	1_3:+71099198	Msa0138230:CDS
GAAATTGACAAAGATCCTGA+AGG	0.616874	1_3:+71099116	Msa0138230:CDS
ATTCATTGGAGGAAAGCTTG+TGG	0.621609	1_3:+71099196	Msa0138230:CDS
GAACGAGTGAACCACTTAGG+TGG	0.634239	1_3:-71099244	None:intergenic
TTCCTCCAATGAATACTGCA+GGG	0.637919	1_3:-71099187	None:intergenic
GAGTGAACCACTTAGGTGGA+GGG	0.652910	1_3:-71099240	None:intergenic
TTAAGATGGAGAAGGTGATG+AGG	0.668317	1_3:+71098988	None:intergenic
TGTCAATTTCATGAACCACA+GGG	0.678104	1_3:-71099103	None:intergenic
AGAAATTGGAGTGTTCCCTG+TGG	0.708961	1_3:+71099088	Msa0138230:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACATTCTGTTTCAAGAAAT+TGG	+	chr1_3:71099074-71099093	Msa0138230:CDS	25.0%
	GAGAAAGCTATAACAAAGTT+GGG	+	chr1_3:71099149-71099168	Msa0138230:CDS	30.0%
	TGTCAATTTCATGAACCACA+GGG	-	chr1_3:71099106-71099125	None:intergenic	35.0%
	TTGTCAATTTCATGAACCAC+AGG	-	chr1_3:71099107-71099126	None:intergenic	35.0%
	GAAATTGACAAAGATCCTGA+AGG	+	chr1_3:71099116-71099135	Msa0138230:CDS	35.0%
	AGATCCTGAAGGAAAAGAAA+TGG	+	chr1_3:71099127-71099146	Msa0138230:CDS	35.0%
!	TTCTCCATTTCTTTTCCTTC+AGG	-	chr1_3:71099134-71099153	None:intergenic	35.0%
	GGAGAAAGCTATAACAAAGT+TGG	+	chr1_3:71099148-71099167	Msa0138230:CDS	35.0%
!	ATGAGGTTGGCTACAGAAAA+GGG	+	chr1_3:71099005-71099024	Msa0138230:CDS	40.0%
	TTCCTCCAATGAATACTGCA+GGG	-	chr1_3:71099190-71099209	None:intergenic	40.0%
	TTTCCTCCAATGAATACTGC+AGG	-	chr1_3:71099191-71099210	None:intergenic	40.0%
	ATTCATTGGAGGAAAGCTTG+TGG	+	chr1_3:71099196-71099215	Msa0138230:CDS	40.0%
	TTCATTGGAGGAAAGCTTGT+GGG	+	chr1_3:71099197-71099216	Msa0138230:CDS	40.0%
!	GATGAGGTTGGCTACAGAAA+AGG	+	chr1_3:71099004-71099023	Msa0138230:CDS	45.0%
	GCACAAACAACAAGAGCTCT+TGG	-	chr1_3:71099045-71099064	None:intergenic	45.0%
!	AGAAATTGGAGTGTTCCCTG+TGG	+	chr1_3:71099088-71099107	Msa0138230:CDS	45.0%
	TCATTGGAGGAAAGCTTGTG+GGG	+	chr1_3:71099198-71099217	Msa0138230:CDS	45.0%
	GAGGGACATTACTTCATTGG+TGG	-	chr1_3:71099225-71099244	None:intergenic	45.0%
	GTGGAGGGACATTACTTCAT+TGG	-	chr1_3:71099228-71099247	None:intergenic	45.0%
	GTTCACTCGTTCCACTACTT+AGG	+	chr1_3:71099255-71099274	Msa0138230:CDS	45.0%
	ATACGGTATGGCCTAAGTAG+TGG	-	chr1_3:71099269-71099288	None:intergenic	45.0%
	CCAATGAATACTGCAGGGAC+AGG	-	chr1_3:71099185-71099204	None:intergenic	50.0%
	CCTGTCCCTGCAGTATTCAT+TGG	+	chr1_3:71099182-71099201	Msa0138230:CDS	50.0%
	GTCCCTGCAGTATTCATTGG+AGG	+	chr1_3:71099185-71099204	Msa0138230:CDS	50.0%
	GTAATGTCCCTCCACCTAAG+TGG	+	chr1_3:71099233-71099252	Msa0138230:CDS	50.0%
	GAGTGAACCACTTAGGTGGA+GGG	-	chr1_3:71099243-71099262	None:intergenic	50.0%
	GAACGAGTGAACCACTTAGG+TGG	-	chr1_3:71099247-71099266	None:intergenic	50.0%
	GTGGAACGAGTGAACCACTT+AGG	-	chr1_3:71099250-71099269	None:intergenic	50.0%
	CGAGTGAACCACTTAGGTGG+AGG	-	chr1_3:71099244-71099263	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	71098993	71099292	71098993	ID=Msa0138230;Name=Msa0138230
chr1_3	mRNA	71098993	71099292	71098993	ID=Msa0138230-mRNA-1;Parent=Msa0138230;Name=Msa0138230-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|99
chr1_3	exon	71098993	71099292	71098993	ID=Msa0138230-mRNA-1:exon:24727;Parent=Msa0138230-mRNA-1
chr1_3	CDS	71098993	71099292	71098993	ID=Msa0138230-mRNA-1:cds;Parent=Msa0138230-mRNA-1

Gene Sequence	>Msa0138230 ATGGAGAAGGTGATGAGGTTGGCTACAGAAAAGGGTGTTGTGATATTCACCAAGAGCTCTTGTTGTTTGTGCTATGCAGTGAACATTCTGTTTCAAGAAATTGGAGTGTTCCCTGTGGTTCATGAAATTGACAAAGATCCTGAAGGAAAAGAAATGGAGAAAGCTATAACAAAGTTGGGTTGTAATGCACCTGTCCCTGCAGTATTCATTGGAGGAAAGCTTGTGGGGTCCACCAATGAAGTAATGTCCCTCCACCTAAGTGGTTCACTCGTTCCACTACTTAGGCCATACCGTATTTAG
Protein sequence	>Msa0138230 MEKVMRLATEKGVVIFTKSSCCLCYAVNILFQEIGVFPVVHEIDKDPEGKEMEKAITKLGCNAPVPAVFIGGKLVGSTNEVMSLHLSGSLVPLLRPYRI*