Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0790240 | sp|Q9M9Y9|GRS11_ARATH | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 1.53e-51 | 160 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0790240 | A0A072VN37 | 98.990 | 99 | 1 | 0 | 1 | 99 | 1 | 99 | 2.76e-65 | 201 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0082540 | Msa0790240 | 0.949000 | 3.074963e-107 | -8.615850e-47 |
| Msa0082550 | Msa0790240 | 0.879395 | 1.290817e-69 | -8.615850e-47 |
| Msa0722930 | Msa0790240 | 0.803088 | 4.118247e-49 | -8.615850e-47 |
| Msa0723140 | Msa0790240 | 0.807666 | 4.522547e-50 | -8.615850e-47 |
| Msa0790230 | Msa0790240 | 0.887823 | 1.017150e-72 | -8.615850e-47 |
| Msa0790240 | Msa1400460 | 0.993721 | 9.708042e-202 | -8.615850e-47 |
| Msa0790240 | Msa1400470 | 0.882426 | 1.052050e-70 | -8.615850e-47 |
| Msa0790240 | Msa1410180 | 0.803242 | 3.826915e-49 | -8.615850e-47 |
| Msa0790240 | Msa1444060 | -0.802437 | 5.612769e-49 | -8.615850e-47 |
| Msa0138230 | Msa0790240 | 0.986830 | 4.072146e-168 | -8.615850e-47 |
| Msa0138240 | Msa0790240 | 0.885813 | 5.883990e-72 | -8.615850e-47 |
| Msa0175630 | Msa0790240 | 0.883890 | 3.059582e-71 | -8.615850e-47 |
| Msa0175640 | Msa0790240 | 0.953364 | 3.225178e-111 | -8.615850e-47 |
| Msa0416800 | Msa0790240 | 0.825544 | 4.424534e-54 | -8.615850e-47 |
| Msa0463460 | Msa0790240 | 0.824558 | 7.561809e-54 | -8.615850e-47 |
| Msa0282600 | Msa0790240 | 0.801365 | 9.318763e-49 | -8.615850e-47 |
| Msa0509020 | Msa0790240 | 0.816546 | 5.229496e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0790240 | MtrunA17_Chr1g0195981 | 98.990 | 99 | 1 | 0 | 1 | 99 | 1 | 99 | 5.32e-69 | 201 |
| Msa0790240 | MtrunA17_Chr1g0195991 | 95.960 | 99 | 4 | 0 | 1 | 99 | 1 | 99 | 4.05e-68 | 199 |
| Msa0790240 | MtrunA17_Chr7g0268711 | 79.592 | 98 | 20 | 0 | 1 | 98 | 1 | 98 | 3.18e-58 | 174 |
| Msa0790240 | MtrunA17_Chr2g0286251 | 58.947 | 95 | 39 | 0 | 1 | 95 | 1 | 95 | 2.11e-39 | 126 |
| Msa0790240 | MtrunA17_Chr1g0195971 | 55.102 | 98 | 44 | 0 | 1 | 98 | 1 | 98 | 4.78e-37 | 120 |
| Msa0790240 | MtrunA17_Chr2g0286271 | 55.789 | 95 | 42 | 0 | 1 | 95 | 1 | 95 | 1.98e-36 | 119 |
| Msa0790240 | MtrunA17_Chr7g0222541 | 54.167 | 96 | 43 | 1 | 1 | 95 | 30 | 125 | 6.06e-35 | 116 |
| Msa0790240 | MtrunA17_Chr7g0222551 | 53.125 | 96 | 44 | 1 | 1 | 95 | 30 | 125 | 1.91e-34 | 115 |
| Msa0790240 | MtrunA17_Chr7g0224091 | 54.167 | 96 | 43 | 1 | 1 | 95 | 22 | 117 | 3.71e-34 | 114 |
| Msa0790240 | MtrunA17_Chr7g0222491 | 53.125 | 96 | 44 | 1 | 1 | 95 | 30 | 125 | 1.78e-33 | 112 |
| Msa0790240 | MtrunA17_Chr4g0066791 | 52.083 | 96 | 45 | 1 | 1 | 95 | 1 | 96 | 1.64e-32 | 109 |
| Msa0790240 | MtrunA17_Chr4g0066801 | 52.083 | 96 | 45 | 1 | 1 | 95 | 1 | 96 | 1.64e-32 | 109 |
| Msa0790240 | MtrunA17_Chr4g0066811 | 52.083 | 96 | 45 | 1 | 1 | 95 | 1 | 96 | 1.64e-32 | 109 |
| Msa0790240 | MtrunA17_Chr7g0268731 | 52.632 | 95 | 44 | 1 | 1 | 94 | 1 | 95 | 1.28e-31 | 107 |
| Msa0790240 | MtrunA17_Chr7g0268721 | 51.579 | 95 | 45 | 1 | 1 | 94 | 1 | 95 | 8.81e-31 | 104 |
| Msa0790240 | MtrunA17_Chr7g0268751 | 43.158 | 95 | 54 | 0 | 1 | 95 | 1 | 95 | 9.16e-26 | 92.0 |
| Msa0790240 | MtrunA17_Chr1g0195951 | 40.426 | 94 | 56 | 0 | 1 | 94 | 1 | 94 | 3.15e-25 | 90.9 |
| Msa0790240 | MtrunA17_Chr1g0195931 | 44.898 | 98 | 54 | 0 | 1 | 98 | 1 | 98 | 1.65e-21 | 81.6 |
| Msa0790240 | MtrunA17_Chr2g0303741 | 41.837 | 98 | 53 | 1 | 2 | 95 | 40 | 137 | 5.06e-21 | 81.3 |
| Msa0790240 | MtrunA17_Chr2g0282951 | 40.426 | 94 | 56 | 0 | 2 | 95 | 27 | 120 | 4.29e-20 | 78.6 |
| Msa0790240 | MtrunA17_Chr7g0268761 | 41.489 | 94 | 55 | 0 | 1 | 94 | 1 | 94 | 4.78e-20 | 77.8 |
| Msa0790240 | MtrunA17_Chr2g0325001 | 36.458 | 96 | 54 | 2 | 4 | 95 | 41 | 133 | 1.52e-18 | 74.7 |
| Msa0790240 | MtrunA17_Chr3g0117411 | 35.789 | 95 | 61 | 0 | 1 | 95 | 18 | 112 | 2.58e-16 | 68.6 |
| Msa0790240 | MtrunA17_Chr4g0040601 | 38.710 | 93 | 52 | 3 | 6 | 95 | 58 | 148 | 7.40e-16 | 68.2 |
| Msa0790240 | MtrunA17_Chr3g0117391 | 34.043 | 94 | 62 | 0 | 1 | 94 | 21 | 114 | 1.06e-15 | 67.0 |
| Msa0790240 | MtrunA17_Chr7g0228311 | 35.106 | 94 | 61 | 0 | 1 | 94 | 3 | 96 | 6.50e-15 | 64.7 |
| Msa0790240 | MtrunA17_Chr1g0151231 | 38.298 | 94 | 56 | 1 | 3 | 94 | 48 | 141 | 1.74e-14 | 64.7 |
| Msa0790240 | MtrunA17_Chr1g0182971 | 32.979 | 94 | 62 | 1 | 2 | 94 | 84 | 177 | 5.08e-14 | 64.3 |
| Msa0790240 | MtrunA17_Chr3g0135811 | 37.634 | 93 | 52 | 3 | 3 | 94 | 23 | 110 | 3.05e-13 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0790240 | AT1G06830.1 | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 1.56e-52 | 160 |
| Msa0790240 | AT2G30540.1 | 70.408 | 98 | 29 | 0 | 1 | 98 | 1 | 98 | 4.21e-52 | 159 |
| Msa0790240 | AT2G47880.1 | 72.449 | 98 | 27 | 0 | 1 | 98 | 1 | 98 | 5.41e-52 | 158 |
| Msa0790240 | AT3G62960.1 | 70.408 | 98 | 29 | 0 | 1 | 98 | 1 | 98 | 2.11e-50 | 154 |
| Msa0790240 | AT3G62950.1 | 51.042 | 96 | 46 | 1 | 1 | 95 | 1 | 96 | 2.22e-36 | 119 |
| Msa0790240 | AT2G47870.1 | 53.125 | 96 | 44 | 1 | 1 | 95 | 1 | 96 | 9.73e-36 | 117 |
| Msa0790240 | AT4G15670.1 | 50.526 | 95 | 47 | 0 | 1 | 95 | 1 | 95 | 3.39e-34 | 114 |
| Msa0790240 | AT3G21460.1 | 54.737 | 95 | 43 | 0 | 1 | 95 | 1 | 95 | 4.27e-34 | 113 |
| Msa0790240 | AT4G15680.1 | 51.579 | 95 | 46 | 0 | 1 | 95 | 1 | 95 | 1.16e-33 | 112 |
| Msa0790240 | AT4G15660.1 | 49.485 | 97 | 49 | 0 | 1 | 97 | 1 | 97 | 1.17e-33 | 112 |
| Msa0790240 | AT4G15700.1 | 51.579 | 95 | 46 | 0 | 1 | 95 | 1 | 95 | 1.30e-33 | 112 |
| Msa0790240 | AT4G15690.1 | 50.526 | 95 | 47 | 0 | 1 | 95 | 1 | 95 | 1.14e-32 | 110 |
| Msa0790240 | AT5G18600.1 | 48.421 | 95 | 49 | 0 | 1 | 95 | 1 | 95 | 1.63e-31 | 107 |
| Msa0790240 | AT5G14070.1 | 45.631 | 103 | 48 | 2 | 1 | 95 | 31 | 133 | 1.31e-26 | 95.9 |
| Msa0790240 | AT3G02000.1 | 45.455 | 99 | 48 | 1 | 3 | 95 | 31 | 129 | 2.60e-25 | 92.4 |
| Msa0790240 | AT3G62930.1 | 43.158 | 95 | 54 | 0 | 1 | 95 | 1 | 95 | 2.93e-25 | 91.3 |
| Msa0790240 | AT1G03020.1 | 45.263 | 95 | 52 | 0 | 1 | 95 | 1 | 95 | 7.03e-25 | 90.1 |
| Msa0790240 | AT4G33040.1 | 42.268 | 97 | 50 | 2 | 3 | 94 | 41 | 136 | 1.61e-17 | 72.8 |
| Msa0790240 | AT1G28480.1 | 36.735 | 98 | 58 | 2 | 2 | 95 | 33 | 130 | 2.62e-17 | 72.0 |
| Msa0790240 | AT5G40370.1 | 32.979 | 94 | 63 | 0 | 1 | 94 | 3 | 96 | 1.01e-14 | 64.7 |
| Msa0790240 | AT5G11930.1 | 41.935 | 93 | 44 | 3 | 3 | 94 | 57 | 140 | 1.55e-14 | 65.1 |
| Msa0790240 | AT4G28730.1 | 35.106 | 94 | 60 | 1 | 2 | 94 | 71 | 164 | 1.66e-14 | 65.5 |
| Msa0790240 | AT5G63030.1 | 36.585 | 82 | 52 | 0 | 13 | 94 | 31 | 112 | 2.68e-12 | 58.9 |
| Msa0790240 | AT2G20270.1 | 34.043 | 94 | 61 | 1 | 2 | 94 | 76 | 169 | 6.84e-12 | 58.9 |
| Msa0790240 | AT5G20500.1 | 31.868 | 91 | 62 | 0 | 4 | 94 | 35 | 125 | 1.49e-11 | 57.0 |
| Msa0790240 | AT4G28730.2 | 37.143 | 70 | 43 | 1 | 2 | 70 | 71 | 140 | 8.55e-11 | 55.1 |
| Msa0790240 | AT1G03850.2 | 34.884 | 86 | 52 | 2 | 10 | 95 | 62 | 143 | 9.75e-11 | 55.1 |
Find 33 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATTCTGTTTCAAGAAAT+TGG | 0.120000 | 5_3:-944595 | Msa0790240:CDS |
| AGATCCTGAAGGAAAAGAAA+TGG | 0.394593 | 5_3:-944542 | Msa0790240:CDS |
| GTGGAGGGACATTACTTCAT+TGG | 0.411453 | 5_3:+944444 | None:intergenic |
| GGAGAAAGCTATAACAAAGT+TGG | 0.418322 | 5_3:-944521 | Msa0790240:CDS |
| CCTGTCCCTGCAGTATTCAT+TGG | 0.431206 | 5_3:-944487 | Msa0790240:CDS |
| GAGAAAGCTATAACAAAGTT+GGG | 0.448427 | 5_3:-944520 | Msa0790240:CDS |
| GATGAGGTTGGCTACAGAAA+AGG | 0.457049 | 5_3:-944665 | Msa0790240:CDS |
| TTCATTGGAGGAAAGCTTGT+GGG | 0.469806 | 5_3:-944472 | Msa0790240:CDS |
| TTTCCTCCAATGAATACTGC+AGG | 0.495376 | 5_3:+944481 | None:intergenic |
| TTGTCAATTTCATGAACCAC+AGG | 0.499275 | 5_3:+944565 | None:intergenic |
| TGATCAAATTAAGCTAAATA+CGG | 0.503808 | 5_3:+944386 | None:intergenic |
| ATGAGGTTGGCTACAGAAAA+GGG | 0.514459 | 5_3:-944664 | Msa0790240:CDS |
| GATGGAGAAGGTGATGAGGT+TGG | 0.515048 | 5_3:-944677 | Msa0790240:CDS |
| GCACAAACAACAAGAGCTCT+TGG | 0.517591 | 5_3:+944627 | None:intergenic |
| CCAATGAATACTGCAGGGAC+AGG | 0.522380 | 5_3:+944487 | None:intergenic |
| GAGGGACATTACTTCATTGG+TGG | 0.543110 | 5_3:+944447 | None:intergenic |
| GTCCCTGCAGTATTCATTGG+AGG | 0.543693 | 5_3:-944484 | Msa0790240:CDS |
| AAATTAAGCTAAATACGGTA+TGG | 0.562707 | 5_3:+944391 | None:intergenic |
| CGAGTGAACCACTTAGGTGG+AGG | 0.574745 | 5_3:+944428 | None:intergenic |
| ATACGGTATGGCCTAAGTAG+TGG | 0.575235 | 5_3:+944403 | None:intergenic |
| GTAATGTCCCTCCACCTAAG+TGG | 0.591093 | 5_3:-944436 | Msa0790240:CDS |
| GTTCACTCGTTCCACTACTT+AGG | 0.591318 | 5_3:-944414 | Msa0790240:CDS |
| GTGGAACGAGTGAACCACTT+AGG | 0.595515 | 5_3:+944422 | None:intergenic |
| CAGTTATATTAAGATGGAGA+AGG | 0.604195 | 5_3:-944689 | None:intergenic |
| TCATTGGAGGAAAGCTTGTG+GGG | 0.613505 | 5_3:-944471 | Msa0790240:CDS |
| GAAATTGACAAAGATCCTGA+AGG | 0.616874 | 5_3:-944553 | Msa0790240:CDS |
| ATTCATTGGAGGAAAGCTTG+TGG | 0.621609 | 5_3:-944473 | Msa0790240:CDS |
| GAACGAGTGAACCACTTAGG+TGG | 0.634239 | 5_3:+944425 | None:intergenic |
| TTCCTCCAATGAATACTGCA+GGG | 0.637919 | 5_3:+944482 | None:intergenic |
| GAGTGAACCACTTAGGTGGA+GGG | 0.652910 | 5_3:+944429 | None:intergenic |
| TTAAGATGGAGAAGGTGATG+AGG | 0.668317 | 5_3:-944681 | None:intergenic |
| TGTCAATTTCATGAACCACA+GGG | 0.678104 | 5_3:+944566 | None:intergenic |
| AGAAATTGGAGTGTTCCCTG+TGG | 0.708961 | 5_3:-944581 | Msa0790240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACATTCTGTTTCAAGAAAT+TGG | - | chr5_3:944480-944499 | Msa0790240:CDS | 25.0% |
| GAGAAAGCTATAACAAAGTT+GGG | - | chr5_3:944555-944574 | Msa0790240:CDS | 30.0% | |
| TGTCAATTTCATGAACCACA+GGG | + | chr5_3:944512-944531 | None:intergenic | 35.0% | |
| TTGTCAATTTCATGAACCAC+AGG | + | chr5_3:944513-944532 | None:intergenic | 35.0% | |
| GAAATTGACAAAGATCCTGA+AGG | - | chr5_3:944522-944541 | Msa0790240:CDS | 35.0% | |
| AGATCCTGAAGGAAAAGAAA+TGG | - | chr5_3:944533-944552 | Msa0790240:CDS | 35.0% | |
| ! | TTCTCCATTTCTTTTCCTTC+AGG | + | chr5_3:944540-944559 | None:intergenic | 35.0% |
| GGAGAAAGCTATAACAAAGT+TGG | - | chr5_3:944554-944573 | Msa0790240:CDS | 35.0% | |
| ! | ATGAGGTTGGCTACAGAAAA+GGG | - | chr5_3:944411-944430 | Msa0790240:CDS | 40.0% |
| TTCCTCCAATGAATACTGCA+GGG | + | chr5_3:944596-944615 | None:intergenic | 40.0% | |
| TTTCCTCCAATGAATACTGC+AGG | + | chr5_3:944597-944616 | None:intergenic | 40.0% | |
| ATTCATTGGAGGAAAGCTTG+TGG | - | chr5_3:944602-944621 | Msa0790240:CDS | 40.0% | |
| TTCATTGGAGGAAAGCTTGT+GGG | - | chr5_3:944603-944622 | Msa0790240:CDS | 40.0% | |
| ! | GATGAGGTTGGCTACAGAAA+AGG | - | chr5_3:944410-944429 | Msa0790240:CDS | 45.0% |
| GCACAAACAACAAGAGCTCT+TGG | + | chr5_3:944451-944470 | None:intergenic | 45.0% | |
| ! | AGAAATTGGAGTGTTCCCTG+TGG | - | chr5_3:944494-944513 | Msa0790240:CDS | 45.0% |
| TCATTGGAGGAAAGCTTGTG+GGG | - | chr5_3:944604-944623 | Msa0790240:CDS | 45.0% | |
| GAGGGACATTACTTCATTGG+TGG | + | chr5_3:944631-944650 | None:intergenic | 45.0% | |
| GTGGAGGGACATTACTTCAT+TGG | + | chr5_3:944634-944653 | None:intergenic | 45.0% | |
| GTTCACTCGTTCCACTACTT+AGG | - | chr5_3:944661-944680 | Msa0790240:CDS | 45.0% | |
| ATACGGTATGGCCTAAGTAG+TGG | + | chr5_3:944675-944694 | None:intergenic | 45.0% | |
| CCAATGAATACTGCAGGGAC+AGG | + | chr5_3:944591-944610 | None:intergenic | 50.0% | |
| CCTGTCCCTGCAGTATTCAT+TGG | - | chr5_3:944588-944607 | Msa0790240:CDS | 50.0% | |
| GTCCCTGCAGTATTCATTGG+AGG | - | chr5_3:944591-944610 | Msa0790240:CDS | 50.0% | |
| GTAATGTCCCTCCACCTAAG+TGG | - | chr5_3:944639-944658 | Msa0790240:CDS | 50.0% | |
| GAGTGAACCACTTAGGTGGA+GGG | + | chr5_3:944649-944668 | None:intergenic | 50.0% | |
| GAACGAGTGAACCACTTAGG+TGG | + | chr5_3:944653-944672 | None:intergenic | 50.0% | |
| GTGGAACGAGTGAACCACTT+AGG | + | chr5_3:944656-944675 | None:intergenic | 50.0% | |
| CGAGTGAACCACTTAGGTGG+AGG | + | chr5_3:944650-944669 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 944399 | 944698 | 944399 | ID=Msa0790240;Name=Msa0790240 |
| chr5_3 | mRNA | 944399 | 944698 | 944399 | ID=Msa0790240-mRNA-1;Parent=Msa0790240;Name=Msa0790240-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|99 |
| chr5_3 | exon | 944399 | 944698 | 944399 | ID=Msa0790240-mRNA-1:exon:343;Parent=Msa0790240-mRNA-1 |
| chr5_3 | CDS | 944399 | 944698 | 944399 | ID=Msa0790240-mRNA-1:cds;Parent=Msa0790240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
