Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400460 | XP_013469214.1 | 98.990 | 99 | 1 | 0 | 1 | 99 | 1 | 99 | 5.78e-65 | 201 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400460 | sp|Q9M9Y9|GRS11_ARATH | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 1.53e-51 | 160 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400460 | A0A072VN37 | 98.990 | 99 | 1 | 0 | 1 | 99 | 1 | 99 | 2.76e-65 | 201 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0082540 | Msa1400460 | 0.959807 | 7.516042e-118 | -8.615850e-47 |
| Msa0082550 | Msa1400460 | 0.884987 | 1.198369e-71 | -8.615850e-47 |
| Msa1400460 | Msa1400470 | 0.887876 | 9.705156e-73 | -8.615850e-47 |
| Msa1400460 | Msa1410180 | 0.804312 | 2.295247e-49 | -8.615850e-47 |
| Msa1400460 | Msa1444060 | -0.800555 | 1.364168e-48 | -8.615850e-47 |
| Msa0722930 | Msa1400460 | 0.807245 | 5.554987e-50 | -8.615850e-47 |
| Msa0723140 | Msa1400460 | 0.810053 | 1.395145e-50 | -8.615850e-47 |
| Msa0790230 | Msa1400460 | 0.893885 | 4.140661e-75 | -8.615850e-47 |
| Msa0790240 | Msa1400460 | 0.993721 | 9.708042e-202 | -8.615850e-47 |
| Msa0138230 | Msa1400460 | 0.993871 | 7.686890e-203 | -8.615850e-47 |
| Msa0138240 | Msa1400460 | 0.890680 | 7.914809e-74 | -8.615850e-47 |
| Msa0175630 | Msa1400460 | 0.889938 | 1.546591e-73 | -8.615850e-47 |
| Msa0175640 | Msa1400460 | 0.949082 | 2.609913e-107 | -8.615850e-47 |
| Msa1249940 | Msa1400460 | 0.805389 | 1.366503e-49 | -8.615850e-47 |
| Msa0416800 | Msa1400460 | 0.830781 | 2.420612e-55 | -8.615850e-47 |
| Msa0463460 | Msa1400460 | 0.827295 | 1.692999e-54 | -8.615850e-47 |
| Msa1055430 | Msa1400460 | 0.800365 | 1.491107e-48 | -8.615850e-47 |
| Msa0282600 | Msa1400460 | 0.805216 | 1.485475e-49 | -8.615850e-47 |
| Msa0509020 | Msa1400460 | 0.819442 | 1.158739e-52 | -8.615850e-47 |
| Msa0843280 | Msa1400460 | 0.802545 | 5.331209e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400460 | MtrunA17_Chr1g0195981 | 98.990 | 99 | 1 | 0 | 1 | 99 | 1 | 99 | 5.32e-69 | 201 |
| Msa1400460 | MtrunA17_Chr1g0195991 | 95.960 | 99 | 4 | 0 | 1 | 99 | 1 | 99 | 4.05e-68 | 199 |
| Msa1400460 | MtrunA17_Chr7g0268711 | 79.592 | 98 | 20 | 0 | 1 | 98 | 1 | 98 | 3.18e-58 | 174 |
| Msa1400460 | MtrunA17_Chr2g0286251 | 58.947 | 95 | 39 | 0 | 1 | 95 | 1 | 95 | 2.11e-39 | 126 |
| Msa1400460 | MtrunA17_Chr1g0195971 | 55.102 | 98 | 44 | 0 | 1 | 98 | 1 | 98 | 4.78e-37 | 120 |
| Msa1400460 | MtrunA17_Chr2g0286271 | 55.789 | 95 | 42 | 0 | 1 | 95 | 1 | 95 | 1.98e-36 | 119 |
| Msa1400460 | MtrunA17_Chr7g0222541 | 54.167 | 96 | 43 | 1 | 1 | 95 | 30 | 125 | 6.06e-35 | 116 |
| Msa1400460 | MtrunA17_Chr7g0222551 | 53.125 | 96 | 44 | 1 | 1 | 95 | 30 | 125 | 1.91e-34 | 115 |
| Msa1400460 | MtrunA17_Chr7g0224091 | 54.167 | 96 | 43 | 1 | 1 | 95 | 22 | 117 | 3.71e-34 | 114 |
| Msa1400460 | MtrunA17_Chr7g0222491 | 53.125 | 96 | 44 | 1 | 1 | 95 | 30 | 125 | 1.78e-33 | 112 |
| Msa1400460 | MtrunA17_Chr4g0066791 | 52.083 | 96 | 45 | 1 | 1 | 95 | 1 | 96 | 1.64e-32 | 109 |
| Msa1400460 | MtrunA17_Chr4g0066801 | 52.083 | 96 | 45 | 1 | 1 | 95 | 1 | 96 | 1.64e-32 | 109 |
| Msa1400460 | MtrunA17_Chr4g0066811 | 52.083 | 96 | 45 | 1 | 1 | 95 | 1 | 96 | 1.64e-32 | 109 |
| Msa1400460 | MtrunA17_Chr7g0268731 | 52.632 | 95 | 44 | 1 | 1 | 94 | 1 | 95 | 1.28e-31 | 107 |
| Msa1400460 | MtrunA17_Chr7g0268721 | 51.579 | 95 | 45 | 1 | 1 | 94 | 1 | 95 | 8.81e-31 | 104 |
| Msa1400460 | MtrunA17_Chr7g0268751 | 43.158 | 95 | 54 | 0 | 1 | 95 | 1 | 95 | 9.16e-26 | 92.0 |
| Msa1400460 | MtrunA17_Chr1g0195951 | 40.426 | 94 | 56 | 0 | 1 | 94 | 1 | 94 | 3.15e-25 | 90.9 |
| Msa1400460 | MtrunA17_Chr1g0195931 | 44.898 | 98 | 54 | 0 | 1 | 98 | 1 | 98 | 1.65e-21 | 81.6 |
| Msa1400460 | MtrunA17_Chr2g0303741 | 41.837 | 98 | 53 | 1 | 2 | 95 | 40 | 137 | 5.06e-21 | 81.3 |
| Msa1400460 | MtrunA17_Chr2g0282951 | 40.426 | 94 | 56 | 0 | 2 | 95 | 27 | 120 | 4.29e-20 | 78.6 |
| Msa1400460 | MtrunA17_Chr7g0268761 | 41.489 | 94 | 55 | 0 | 1 | 94 | 1 | 94 | 4.78e-20 | 77.8 |
| Msa1400460 | MtrunA17_Chr2g0325001 | 36.458 | 96 | 54 | 2 | 4 | 95 | 41 | 133 | 1.52e-18 | 74.7 |
| Msa1400460 | MtrunA17_Chr3g0117411 | 35.789 | 95 | 61 | 0 | 1 | 95 | 18 | 112 | 2.58e-16 | 68.6 |
| Msa1400460 | MtrunA17_Chr4g0040601 | 38.710 | 93 | 52 | 3 | 6 | 95 | 58 | 148 | 7.40e-16 | 68.2 |
| Msa1400460 | MtrunA17_Chr3g0117391 | 34.043 | 94 | 62 | 0 | 1 | 94 | 21 | 114 | 1.06e-15 | 67.0 |
| Msa1400460 | MtrunA17_Chr7g0228311 | 35.106 | 94 | 61 | 0 | 1 | 94 | 3 | 96 | 6.50e-15 | 64.7 |
| Msa1400460 | MtrunA17_Chr1g0151231 | 38.298 | 94 | 56 | 1 | 3 | 94 | 48 | 141 | 1.74e-14 | 64.7 |
| Msa1400460 | MtrunA17_Chr1g0182971 | 32.979 | 94 | 62 | 1 | 2 | 94 | 84 | 177 | 5.08e-14 | 64.3 |
| Msa1400460 | MtrunA17_Chr3g0135811 | 37.634 | 93 | 52 | 3 | 3 | 94 | 23 | 110 | 3.05e-13 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400460 | AT1G06830.1 | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 1.56e-52 | 160 |
| Msa1400460 | AT2G30540.1 | 70.408 | 98 | 29 | 0 | 1 | 98 | 1 | 98 | 4.21e-52 | 159 |
| Msa1400460 | AT2G47880.1 | 72.449 | 98 | 27 | 0 | 1 | 98 | 1 | 98 | 5.41e-52 | 158 |
| Msa1400460 | AT3G62960.1 | 70.408 | 98 | 29 | 0 | 1 | 98 | 1 | 98 | 2.11e-50 | 154 |
| Msa1400460 | AT3G62950.1 | 51.042 | 96 | 46 | 1 | 1 | 95 | 1 | 96 | 2.22e-36 | 119 |
| Msa1400460 | AT2G47870.1 | 53.125 | 96 | 44 | 1 | 1 | 95 | 1 | 96 | 9.73e-36 | 117 |
| Msa1400460 | AT4G15670.1 | 50.526 | 95 | 47 | 0 | 1 | 95 | 1 | 95 | 3.39e-34 | 114 |
| Msa1400460 | AT3G21460.1 | 54.737 | 95 | 43 | 0 | 1 | 95 | 1 | 95 | 4.27e-34 | 113 |
| Msa1400460 | AT4G15680.1 | 51.579 | 95 | 46 | 0 | 1 | 95 | 1 | 95 | 1.16e-33 | 112 |
| Msa1400460 | AT4G15660.1 | 49.485 | 97 | 49 | 0 | 1 | 97 | 1 | 97 | 1.17e-33 | 112 |
| Msa1400460 | AT4G15700.1 | 51.579 | 95 | 46 | 0 | 1 | 95 | 1 | 95 | 1.30e-33 | 112 |
| Msa1400460 | AT4G15690.1 | 50.526 | 95 | 47 | 0 | 1 | 95 | 1 | 95 | 1.14e-32 | 110 |
| Msa1400460 | AT5G18600.1 | 48.421 | 95 | 49 | 0 | 1 | 95 | 1 | 95 | 1.63e-31 | 107 |
| Msa1400460 | AT5G14070.1 | 45.631 | 103 | 48 | 2 | 1 | 95 | 31 | 133 | 1.31e-26 | 95.9 |
| Msa1400460 | AT3G02000.1 | 45.455 | 99 | 48 | 1 | 3 | 95 | 31 | 129 | 2.60e-25 | 92.4 |
| Msa1400460 | AT3G62930.1 | 43.158 | 95 | 54 | 0 | 1 | 95 | 1 | 95 | 2.93e-25 | 91.3 |
| Msa1400460 | AT1G03020.1 | 45.263 | 95 | 52 | 0 | 1 | 95 | 1 | 95 | 7.03e-25 | 90.1 |
| Msa1400460 | AT4G33040.1 | 42.268 | 97 | 50 | 2 | 3 | 94 | 41 | 136 | 1.61e-17 | 72.8 |
| Msa1400460 | AT1G28480.1 | 36.735 | 98 | 58 | 2 | 2 | 95 | 33 | 130 | 2.62e-17 | 72.0 |
| Msa1400460 | AT5G40370.1 | 32.979 | 94 | 63 | 0 | 1 | 94 | 3 | 96 | 1.01e-14 | 64.7 |
| Msa1400460 | AT5G11930.1 | 41.935 | 93 | 44 | 3 | 3 | 94 | 57 | 140 | 1.55e-14 | 65.1 |
| Msa1400460 | AT4G28730.1 | 35.106 | 94 | 60 | 1 | 2 | 94 | 71 | 164 | 1.66e-14 | 65.5 |
| Msa1400460 | AT5G63030.1 | 36.585 | 82 | 52 | 0 | 13 | 94 | 31 | 112 | 2.68e-12 | 58.9 |
| Msa1400460 | AT2G20270.1 | 34.043 | 94 | 61 | 1 | 2 | 94 | 76 | 169 | 6.84e-12 | 58.9 |
| Msa1400460 | AT5G20500.1 | 31.868 | 91 | 62 | 0 | 4 | 94 | 35 | 125 | 1.49e-11 | 57.0 |
| Msa1400460 | AT4G28730.2 | 37.143 | 70 | 43 | 1 | 2 | 70 | 71 | 140 | 8.55e-11 | 55.1 |
| Msa1400460 | AT1G03850.2 | 34.884 | 86 | 52 | 2 | 10 | 95 | 62 | 143 | 9.75e-11 | 55.1 |
Find 33 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATTCTGTTTCAAGAAAT+TGG | 0.120000 | tig0022456:+41222 | Msa1400460:CDS |
| AGATCCTGAAGGAAAAGAAA+TGG | 0.394593 | tig0022456:+41275 | Msa1400460:CDS |
| GTGGAGGGACATTACTTCAT+TGG | 0.411453 | tig0022456:-41373 | None:intergenic |
| GGAGAAAGCTATAACAAAGT+TGG | 0.418322 | tig0022456:+41296 | Msa1400460:CDS |
| CCTGTCCCTGCAGTATTCAT+TGG | 0.431206 | tig0022456:+41330 | Msa1400460:CDS |
| GAGAAAGCTATAACAAAGTT+GGG | 0.448427 | tig0022456:+41297 | Msa1400460:CDS |
| GATGAGGTTGGCTACAGAAA+AGG | 0.457049 | tig0022456:+41152 | Msa1400460:CDS |
| TTCATTGGAGGAAAGCTTGT+GGG | 0.469806 | tig0022456:+41345 | Msa1400460:CDS |
| TTTCCTCCAATGAATACTGC+AGG | 0.495376 | tig0022456:-41336 | None:intergenic |
| TTGTCAATTTCATGAACCAC+AGG | 0.499275 | tig0022456:-41252 | None:intergenic |
| TGATCAAATTAAGCTAAATA+CGG | 0.503808 | tig0022456:-41431 | None:intergenic |
| ATGAGGTTGGCTACAGAAAA+GGG | 0.514459 | tig0022456:+41153 | Msa1400460:CDS |
| GATGGAGAAGGTGATGAGGT+TGG | 0.515048 | tig0022456:+41140 | None:intergenic |
| GCACAAACAACAAGAGCTCT+TGG | 0.517591 | tig0022456:-41190 | None:intergenic |
| CCAATGAATACTGCAGGGAC+AGG | 0.522380 | tig0022456:-41330 | None:intergenic |
| GAGGGACATTACTTCATTGG+TGG | 0.543110 | tig0022456:-41370 | None:intergenic |
| GTCCCTGCAGTATTCATTGG+AGG | 0.543693 | tig0022456:+41333 | Msa1400460:CDS |
| AAATTAAGCTAAATACGGTA+TGG | 0.562707 | tig0022456:-41426 | None:intergenic |
| CGAGTGAACCACTTAGGTGG+AGG | 0.574745 | tig0022456:-41389 | None:intergenic |
| ATACGGTATGGCCTAAGTAG+TGG | 0.575235 | tig0022456:-41414 | None:intergenic |
| GTAATGTCCCTCCACCTAAG+TGG | 0.591093 | tig0022456:+41381 | Msa1400460:CDS |
| GTTCACTCGTTCCACTACTT+AGG | 0.591318 | tig0022456:+41403 | Msa1400460:CDS |
| GTGGAACGAGTGAACCACTT+AGG | 0.595515 | tig0022456:-41395 | None:intergenic |
| CAGTTATATTAAGATGGAGA+AGG | 0.604195 | tig0022456:+41128 | None:intergenic |
| TCATTGGAGGAAAGCTTGTG+GGG | 0.613505 | tig0022456:+41346 | Msa1400460:CDS |
| GAAATTGACAAAGATCCTGA+AGG | 0.616874 | tig0022456:+41264 | Msa1400460:CDS |
| ATTCATTGGAGGAAAGCTTG+TGG | 0.621609 | tig0022456:+41344 | Msa1400460:CDS |
| GAACGAGTGAACCACTTAGG+TGG | 0.634239 | tig0022456:-41392 | None:intergenic |
| TTCCTCCAATGAATACTGCA+GGG | 0.637919 | tig0022456:-41335 | None:intergenic |
| GAGTGAACCACTTAGGTGGA+GGG | 0.652910 | tig0022456:-41388 | None:intergenic |
| TTAAGATGGAGAAGGTGATG+AGG | 0.668317 | tig0022456:+41136 | None:intergenic |
| TGTCAATTTCATGAACCACA+GGG | 0.678104 | tig0022456:-41251 | None:intergenic |
| AGAAATTGGAGTGTTCCCTG+TGG | 0.708961 | tig0022456:+41236 | Msa1400460:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACATTCTGTTTCAAGAAAT+TGG | + | tig0022456:41222-41241 | Msa1400460:CDS | 25.0% |
| GAGAAAGCTATAACAAAGTT+GGG | + | tig0022456:41297-41316 | Msa1400460:CDS | 30.0% | |
| TGTCAATTTCATGAACCACA+GGG | - | tig0022456:41254-41273 | None:intergenic | 35.0% | |
| TTGTCAATTTCATGAACCAC+AGG | - | tig0022456:41255-41274 | None:intergenic | 35.0% | |
| GAAATTGACAAAGATCCTGA+AGG | + | tig0022456:41264-41283 | Msa1400460:CDS | 35.0% | |
| AGATCCTGAAGGAAAAGAAA+TGG | + | tig0022456:41275-41294 | Msa1400460:CDS | 35.0% | |
| ! | TTCTCCATTTCTTTTCCTTC+AGG | - | tig0022456:41282-41301 | None:intergenic | 35.0% |
| GGAGAAAGCTATAACAAAGT+TGG | + | tig0022456:41296-41315 | Msa1400460:CDS | 35.0% | |
| ! | ATGAGGTTGGCTACAGAAAA+GGG | + | tig0022456:41153-41172 | Msa1400460:CDS | 40.0% |
| TTCCTCCAATGAATACTGCA+GGG | - | tig0022456:41338-41357 | None:intergenic | 40.0% | |
| TTTCCTCCAATGAATACTGC+AGG | - | tig0022456:41339-41358 | None:intergenic | 40.0% | |
| ATTCATTGGAGGAAAGCTTG+TGG | + | tig0022456:41344-41363 | Msa1400460:CDS | 40.0% | |
| TTCATTGGAGGAAAGCTTGT+GGG | + | tig0022456:41345-41364 | Msa1400460:CDS | 40.0% | |
| ! | GATGAGGTTGGCTACAGAAA+AGG | + | tig0022456:41152-41171 | Msa1400460:CDS | 45.0% |
| GCACAAACAACAAGAGCTCT+TGG | - | tig0022456:41193-41212 | None:intergenic | 45.0% | |
| ! | AGAAATTGGAGTGTTCCCTG+TGG | + | tig0022456:41236-41255 | Msa1400460:CDS | 45.0% |
| TCATTGGAGGAAAGCTTGTG+GGG | + | tig0022456:41346-41365 | Msa1400460:CDS | 45.0% | |
| GAGGGACATTACTTCATTGG+TGG | - | tig0022456:41373-41392 | None:intergenic | 45.0% | |
| GTGGAGGGACATTACTTCAT+TGG | - | tig0022456:41376-41395 | None:intergenic | 45.0% | |
| GTTCACTCGTTCCACTACTT+AGG | + | tig0022456:41403-41422 | Msa1400460:CDS | 45.0% | |
| ATACGGTATGGCCTAAGTAG+TGG | - | tig0022456:41417-41436 | None:intergenic | 45.0% | |
| CCAATGAATACTGCAGGGAC+AGG | - | tig0022456:41333-41352 | None:intergenic | 50.0% | |
| CCTGTCCCTGCAGTATTCAT+TGG | + | tig0022456:41330-41349 | Msa1400460:CDS | 50.0% | |
| GTCCCTGCAGTATTCATTGG+AGG | + | tig0022456:41333-41352 | Msa1400460:CDS | 50.0% | |
| GTAATGTCCCTCCACCTAAG+TGG | + | tig0022456:41381-41400 | Msa1400460:CDS | 50.0% | |
| GAGTGAACCACTTAGGTGGA+GGG | - | tig0022456:41391-41410 | None:intergenic | 50.0% | |
| GAACGAGTGAACCACTTAGG+TGG | - | tig0022456:41395-41414 | None:intergenic | 50.0% | |
| GTGGAACGAGTGAACCACTT+AGG | - | tig0022456:41398-41417 | None:intergenic | 50.0% | |
| CGAGTGAACCACTTAGGTGG+AGG | - | tig0022456:41392-41411 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022456 | gene | 41141 | 41440 | 41141 | ID=Msa1400460;Name=Msa1400460 |
| tig0022456 | mRNA | 41141 | 41440 | 41141 | ID=Msa1400460-mRNA-1;Parent=Msa1400460;Name=Msa1400460-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|99 |
| tig0022456 | exon | 41141 | 41440 | 41141 | ID=Msa1400460-mRNA-1:exon:17725;Parent=Msa1400460-mRNA-1 |
| tig0022456 | CDS | 41141 | 41440 | 41141 | ID=Msa1400460-mRNA-1:cds;Parent=Msa1400460-mRNA-1 |
| Gene Sequence |
| Protein sequence |
