AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0180390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180390 MtrunA17_Chr1g0206601 91.698 265 22 0 1 265 22 286 0.0 513
Msa0180390 MtrunA17_Chr1g0206581 81.387 274 41 2 1 265 18 290 8.42e-163 452
Msa0180390 MtrunA17_Chr1g0206651 73.047 256 63 4 1 255 1 251 3.24e-135 381
Msa0180390 MtrunA17_Chr1g0206571 62.343 239 90 0 20 258 23 261 3.21e-108 315
Msa0180390 MtrunA17_Chr1g0154361 61.753 251 91 3 10 255 3 253 1.02e-105 309
Msa0180390 MtrunA17_Chr1g0154341 56.923 260 107 3 10 264 3 262 4.05e-100 293
Msa0180390 MtrunA17_Chr1g0154311 54.839 248 108 2 14 258 10 256 3.25e-93 288
Msa0180390 MtrunA17_Chr1g0154371 55.769 260 108 4 1 254 8 266 4.33e-93 288
Msa0180390 MtrunA17_Chr1g0154351 55.952 252 106 3 10 256 3 254 7.39e-91 282
Msa0180390 MtrunA17_Chr1g0154321 54.902 255 106 5 10 255 3 257 1.54e-86 273
Msa0180390 MtrunA17_Chr1g0206531 50.980 255 113 6 4 256 8 252 8.06e-79 251
Msa0180390 MtrunA17_Chr5g0428591 48.780 246 118 5 10 255 8 245 2.63e-72 223
Msa0180390 MtrunA17_Chr1g0206461 48.235 255 121 5 2 256 6 249 1.28e-71 231
Msa0180390 MtrunA17_Chr5g0428571 50.909 220 104 3 40 259 35 250 2.81e-66 214
Msa0180390 MtrunA17_Chr5g0428531 45.669 254 115 7 10 254 8 247 9.40e-65 204
Msa0180390 MtrunA17_Chr1g0206451 48.828 256 122 5 2 256 40 287 1.29e-62 208
Msa0180390 MtrunA17_Chr1g0206521 47.843 255 123 5 2 256 648 892 1.30e-61 209
Msa0180390 MtrunA17_Chr1g0206521 47.718 241 117 5 16 256 19 250 1.70e-59 203
Msa0180390 MtrunA17_Chr1g0206561 40.873 252 140 4 11 262 4 246 8.85e-52 179
Msa0180390 MtrunA17_Chr5g0426941 40.304 263 138 7 4 262 1 248 5.54e-51 176
Msa0180390 MtrunA17_Chr1g0206381 37.917 240 135 6 24 255 19 252 7.76e-44 151
Msa0180390 MtrunA17_Chr1g0206401 43.256 215 107 8 43 248 33 241 3.60e-43 155
Msa0180390 MtrunA17_Chr2g0323011 31.452 248 158 7 4 248 1 239 1.92e-34 124
Msa0180390 MtrunA17_Chr1g0205771 33.617 235 145 6 24 255 33 259 1.71e-32 125
Msa0180390 MtrunA17_Chr1g0206331 34.562 217 133 7 44 255 41 253 7.93e-32 123
Msa0180390 MtrunA17_Chr5g0428601 47.619 126 63 3 130 255 1 123 1.53e-30 117
Msa0180390 MtrunA17_Chr6g0475001 33.730 252 153 8 11 255 7 251 1.89e-30 119
Msa0180390 MtrunA17_Chr6g0475001 34.184 196 113 8 68 254 301 489 8.32e-18 82.8
Msa0180390 MtrunA17_Chr7g0234231 29.839 248 165 5 9 256 1 239 1.93e-30 114
Msa0180390 MtrunA17_Chr6g0475121 32.046 259 162 7 9 261 7 257 7.89e-29 114
Msa0180390 MtrunA17_Chr6g0475121 28.992 238 150 10 21 254 268 490 2.30e-18 84.3
Msa0180390 MtrunA17_Chr6g0474901 31.776 214 137 5 47 255 35 244 1.84e-28 114
Msa0180390 MtrunA17_Chr6g0474901 30.864 243 142 11 23 254 256 483 2.37e-19 87.4
Msa0180390 MtrunA17_Chr5g0394001 32.114 246 155 8 13 254 12 249 2.21e-28 113
Msa0180390 MtrunA17_Chr6g0474971 33.178 214 134 5 47 255 35 244 3.97e-28 109
Msa0180390 MtrunA17_Chr1g0206201 32.271 251 156 9 4 248 6 248 6.39e-27 105
Msa0180390 MtrunA17_Chr1g0206361 32.735 223 139 7 40 258 42 257 7.41e-27 109
Msa0180390 MtrunA17_Chr6g0475201 30.303 264 173 6 1 261 1 256 4.28e-26 107
Msa0180390 MtrunA17_Chr6g0475201 35.714 168 101 6 89 254 328 490 6.49e-23 97.8
Msa0180390 MtrunA17_Chr8g0347571 29.455 275 161 8 9 263 7 268 5.93e-26 106
Msa0180390 MtrunA17_Chr8g0347571 26.953 256 157 12 23 265 261 499 1.03e-17 82.8
Msa0180390 MtrunA17_Chr6g0474811 31.203 266 155 10 4 255 1 252 5.99e-26 106
Msa0180390 MtrunA17_Chr6g0474811 32.500 200 122 7 68 259 303 497 5.00e-20 89.4
Msa0180390 MtrunA17_Chr1g0206351 29.870 231 152 5 28 255 31 254 1.55e-25 105
Msa0180390 MtrunA17_Chr6g0474871 31.076 251 158 8 12 255 7 249 2.93e-25 104
Msa0180390 MtrunA17_Chr6g0474871 31.746 189 116 7 74 254 304 487 1.81e-18 84.7
Msa0180390 MtrunA17_Chr2g0322981 29.796 245 154 8 6 248 11 239 6.41e-25 99.4
Msa0180390 MtrunA17_Chr6g0475111 29.317 249 167 6 9 254 10 252 7.70e-25 103
Msa0180390 MtrunA17_Chr6g0475111 29.070 172 111 6 89 255 337 502 8.44e-17 80.1
Msa0180390 MtrunA17_Chr5g0394021 33.880 183 116 3 72 254 70 247 1.29e-24 102
Msa0180390 MtrunA17_Chr5g0393981 31.193 218 129 9 45 254 113 317 3.33e-24 101
Msa0180390 MtrunA17_Chr3g0081001 27.341 267 175 6 3 265 5 256 3.45e-24 101
Msa0180390 MtrunA17_Chr1g0206231 32.536 209 131 7 51 255 52 254 8.16e-24 100
Msa0180390 MtrunA17_Chr6g0474861 32.932 249 150 11 11 254 7 243 1.51e-23 99.8
Msa0180390 MtrunA17_Chr6g0475091 31.797 217 139 5 44 255 48 260 1.78e-23 99.8
Msa0180390 MtrunA17_Chr6g0475091 33.889 180 112 6 82 259 330 504 5.37e-18 83.6
Msa0180390 MtrunA17_Chr1g0206291 30.435 253 158 9 11 255 23 265 1.90e-23 99.0
Msa0180390 MtrunA17_Chr5g0394011 31.019 216 138 7 45 254 41 251 2.58e-23 99.0
Msa0180390 MtrunA17_Chr3g0081051 32.547 212 134 5 41 249 31 236 5.55e-23 94.7
Msa0180390 MtrunA17_Chr3g0081011 28.182 220 144 6 50 265 46 255 6.37e-23 97.8
Msa0180390 MtrunA17_Chr3g0081031 31.967 244 152 6 9 249 22 254 1.54e-22 96.7
Msa0180390 MtrunA17_Chr8g0354271 27.027 259 178 7 9 264 8 258 3.04e-21 92.8
Msa0180390 MtrunA17_Chr8g0354421 29.885 261 157 13 9 259 30 274 5.13e-20 89.4
Msa0180390 MtrunA17_Chr8g0354441 27.407 270 167 12 9 265 13 266 7.79e-20 89.0
Msa0180390 MtrunA17_Chr8g0354351 26.154 260 176 8 9 265 7 253 1.89e-19 87.8
Msa0180390 MtrunA17_Chr8g0354411 27.979 193 129 6 75 265 74 258 4.03e-18 82.4
Msa0180390 MtrunA17_Chr8g0354341 31.694 183 114 6 75 255 73 246 1.63e-17 79.7
Msa0180390 MtrunA17_Chr3g0119041 29.384 211 124 7 68 259 63 267 4.89e-17 80.5
Msa0180390 MtrunA17_Chr1g0206251 34.254 181 107 7 82 254 333 509 5.06e-17 80.5
Msa0180390 MtrunA17_Chr1g0206251 26.667 255 171 7 11 256 19 266 5.80e-13 68.6
Msa0180390 MtrunA17_Chr1g0206421 30.189 159 94 5 71 220 2 152 8.26e-17 79.7
Msa0180390 MtrunA17_Chr2g0280391 25.941 239 165 7 25 262 27 254 2.20e-16 77.0
Msa0180390 MtrunA17_Chr2g0280361 27.197 239 157 7 25 262 27 249 2.24e-16 76.6
Msa0180390 MtrunA17_Chr1g0206411 53.448 58 27 0 65 122 4 61 4.83e-15 68.9
Msa0180390 MtrunA17_Chr8g0354431 23.938 259 187 6 9 265 8 258 1.68e-12 67.0
Msa0180390 MtrunA17_Chr8g0354281 24.786 234 150 9 32 255 30 247 3.51e-12 66.2
Msa0180390 MtrunA17_Chr2g0280381 25.837 209 143 5 55 262 51 248 1.43e-11 63.2
Msa0180390 MtrunA17_Chr1g0206541 44.643 56 30 1 208 263 19 73 6.30e-11 58.2
Msa0180390 MtrunA17_Chr3g0082951 33.613 119 75 3 22 139 106 221 7.25e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180390 AT4G21410.2 36.719 256 152 6 13 265 13 261 2.47e-42 151
Msa0180390 AT4G21410.3 36.719 256 152 6 13 265 13 261 9.48e-42 152
Msa0180390 AT4G38830.1 37.273 220 132 5 39 256 32 247 5.58e-37 138
Msa0180390 AT4G05200.2 36.145 249 139 9 11 254 33 266 1.62e-36 137
Msa0180390 AT4G05200.1 36.145 249 139 9 11 254 15 248 1.84e-36 137
Msa0180390 AT4G21400.3 34.667 225 142 4 43 265 42 263 8.03e-36 134
Msa0180390 AT4G21400.6 34.667 225 142 4 43 265 42 263 9.32e-36 134
Msa0180390 AT4G21400.4 34.667 225 142 4 43 265 42 263 9.32e-36 134
Msa0180390 AT4G21400.5 34.667 225 142 4 43 265 42 263 9.32e-36 134
Msa0180390 AT4G21400.2 33.071 254 163 5 14 265 15 263 2.06e-35 134
Msa0180390 AT4G21400.1 34.667 225 142 4 43 265 42 263 2.23e-35 134
Msa0180390 AT3G22060.1 32.500 240 154 5 9 248 13 244 3.44e-35 127
Msa0180390 AT4G00970.2 39.320 206 113 6 53 254 65 262 1.36e-32 125
Msa0180390 AT4G00970.1 39.320 206 113 6 53 254 65 262 2.73e-32 125
Msa0180390 AT4G21400.7 35.829 187 116 3 80 265 54 237 1.18e-31 123
Msa0180390 AT4G23170.1 38.249 217 117 9 45 254 39 245 1.91e-31 117
Msa0180390 AT4G21230.1 35.897 234 125 9 41 263 37 256 1.93e-31 122
Msa0180390 AT4G23220.1 32.212 208 139 2 43 248 29 236 1.21e-30 120
Msa0180390 AT4G23180.3 36.752 234 132 9 29 258 36 257 2.40e-30 119
Msa0180390 AT4G23180.2 36.752 234 132 9 29 258 36 257 4.11e-30 118
Msa0180390 AT4G23180.1 36.752 234 132 9 29 258 36 257 4.78e-30 119
Msa0180390 AT4G23280.1 37.383 214 122 6 44 254 38 242 8.15e-30 118
Msa0180390 AT4G23280.3 37.383 214 122 6 44 254 38 242 8.83e-30 117
Msa0180390 AT4G23280.2 37.383 214 122 6 44 254 38 242 9.47e-30 117
Msa0180390 AT4G23160.3 36.771 223 128 7 41 258 632 846 1.47e-29 117
Msa0180390 AT4G23160.2 36.771 223 128 7 41 258 632 846 1.47e-29 117
Msa0180390 AT4G23160.1 36.771 223 128 7 41 258 52 266 1.64e-29 117
Msa0180390 AT4G23130.3 35.577 208 123 7 51 255 46 245 7.03e-29 115
Msa0180390 AT4G23150.1 34.728 239 143 7 24 258 20 249 8.11e-29 115
Msa0180390 AT4G23130.1 35.577 208 123 7 51 255 46 245 9.46e-29 115
Msa0180390 AT4G23130.2 35.577 208 123 7 51 255 46 245 9.52e-29 115
Msa0180390 AT4G23210.2 32.889 225 128 6 40 248 29 246 2.26e-28 113
Msa0180390 AT4G23210.4 32.889 225 128 6 40 248 29 246 2.26e-28 113
Msa0180390 AT4G23210.3 32.889 225 128 6 40 248 29 246 2.75e-28 114
Msa0180390 AT4G23210.5 32.889 225 128 6 40 248 29 246 2.90e-28 113
Msa0180390 AT4G23210.1 32.889 225 128 6 40 248 29 246 3.01e-28 113
Msa0180390 AT4G23300.1 33.036 224 127 6 41 248 30 246 4.45e-28 113
Msa0180390 AT2G31620.1 30.942 223 141 6 40 256 40 255 4.71e-28 108
Msa0180390 AT4G23200.2 29.524 210 142 2 45 248 30 239 8.48e-28 111
Msa0180390 AT4G23200.1 29.524 210 142 2 45 248 30 239 1.24e-27 112
Msa0180390 AT4G23140.2 35.176 199 115 6 65 258 61 250 1.23e-26 108
Msa0180390 AT4G23140.1 35.176 199 115 6 65 258 61 250 1.44e-26 108
Msa0180390 AT4G11521.1 28.889 225 147 3 32 248 18 237 7.59e-26 102
Msa0180390 AT4G11460.3 31.193 218 135 5 43 248 32 246 5.48e-25 104
Msa0180390 AT4G11460.1 31.193 218 135 5 43 248 32 246 5.50e-25 104
Msa0180390 AT4G11530.1 26.506 249 163 4 9 248 5 242 7.46e-25 103
Msa0180390 AT3G45860.1 37.186 199 109 7 65 258 64 251 7.75e-25 103
Msa0180390 AT4G23230.1 32.195 205 132 4 51 254 43 241 3.49e-24 102
Msa0180390 AT5G41290.1 29.150 247 156 8 12 249 7 243 3.62e-24 98.6
Msa0180390 AT4G23190.1 31.250 224 133 6 39 248 28 244 7.14e-24 100
Msa0180390 AT4G23190.2 31.250 224 133 6 39 248 28 244 8.02e-24 100
Msa0180390 AT4G23270.2 32.540 252 152 10 7 254 3 240 1.22e-23 100
Msa0180390 AT4G23270.1 32.540 252 152 10 7 254 3 240 1.22e-23 100
Msa0180390 AT4G23270.3 32.540 252 152 10 7 254 3 240 1.32e-23 100
Msa0180390 AT4G20640.1 28.112 249 168 5 5 248 7 249 1.33e-23 96.7
Msa0180390 AT4G20630.1 28.112 249 168 5 5 248 7 249 1.33e-23 96.7
Msa0180390 AT4G20580.1 28.112 249 168 5 5 248 7 249 1.33e-23 96.7
Msa0180390 AT4G20600.1 28.112 249 168 5 5 248 7 249 1.33e-23 96.7
Msa0180390 AT4G20620.1 28.112 249 168 5 5 248 7 249 1.33e-23 96.7
Msa0180390 AT4G20610.1 28.112 249 168 5 5 248 7 249 1.33e-23 96.7
Msa0180390 AT4G20590.1 28.112 249 168 5 5 248 7 249 1.33e-23 96.7
Msa0180390 AT3G21960.1 27.490 251 171 6 3 248 11 255 1.52e-23 96.7
Msa0180390 AT4G20570.1 28.112 249 168 5 5 248 21 263 1.66e-23 96.7
Msa0180390 AT4G20560.1 28.112 249 168 5 5 248 21 263 1.66e-23 96.7
Msa0180390 AT4G20540.1 28.112 249 168 5 5 248 21 263 1.66e-23 96.7
Msa0180390 AT4G20645.1 28.112 249 168 5 5 248 21 263 1.66e-23 96.7
Msa0180390 AT4G20530.1 28.112 249 168 5 5 248 21 263 1.66e-23 96.7
Msa0180390 AT4G20550.1 28.112 249 168 5 5 248 21 263 1.66e-23 96.7
Msa0180390 AT4G20670.1 28.112 249 168 5 5 248 7 249 1.85e-23 96.3
Msa0180390 AT4G23250.1 28.692 237 141 7 36 254 30 256 6.13e-23 98.2
Msa0180390 AT4G23250.3 28.692 237 141 7 36 254 30 256 6.50e-23 98.2
Msa0180390 AT4G23250.2 28.692 237 141 7 36 254 30 256 7.08e-23 98.2
Msa0180390 AT4G23250.2 27.358 212 129 5 43 254 733 919 1.37e-13 70.9
Msa0180390 AT3G58310.1 26.556 241 171 4 9 248 14 249 8.33e-23 94.4
Msa0180390 AT3G21990.1 27.309 249 170 5 5 248 7 249 1.14e-22 94.0
Msa0180390 AT1G61750.1 30.660 212 139 6 43 251 38 244 3.36e-22 94.4
Msa0180390 AT4G23240.1 29.630 216 145 4 43 254 33 245 4.73e-22 95.5
Msa0180390 AT4G21410.1 40.580 138 78 3 130 265 1 136 5.95e-22 95.1
Msa0180390 AT4G11490.1 29.493 217 145 3 38 248 27 241 2.97e-21 93.2
Msa0180390 AT3G21910.1 28.634 227 148 7 42 258 48 270 1.00e-20 89.4
Msa0180390 AT4G23310.2 32.512 203 124 6 50 248 157 350 1.24e-20 91.7
Msa0180390 AT4G23310.1 32.512 203 124 6 50 248 157 350 1.34e-20 91.7
Msa0180390 AT5G48540.1 29.600 250 155 8 13 256 9 243 1.72e-20 88.2
Msa0180390 AT4G23260.2 28.636 220 149 5 36 249 30 247 4.41e-20 89.7
Msa0180390 AT4G21400.8 37.226 137 84 2 130 265 1 136 4.49e-20 89.7
Msa0180390 AT3G21920.1 30.204 245 141 11 32 258 40 272 4.84e-20 87.4
Msa0180390 AT4G23260.1 28.636 220 149 5 36 249 30 247 5.26e-20 89.7
Msa0180390 AT3G21920.2 30.204 245 141 11 32 258 62 294 5.37e-20 87.8
Msa0180390 AT4G04500.2 31.250 208 128 5 61 255 49 254 1.08e-19 88.6
Msa0180390 AT4G04500.3 31.250 208 128 5 61 255 49 254 1.08e-19 88.6
Msa0180390 AT4G04500.1 31.250 208 128 5 61 255 49 254 1.19e-19 88.6
Msa0180390 AT1G63550.1 28.972 214 145 4 40 250 37 246 1.21e-19 87.0
Msa0180390 AT1G63600.1 28.054 221 149 4 35 250 39 254 1.21e-19 87.0
Msa0180390 AT3G21930.1 29.796 245 142 11 32 258 40 272 1.67e-19 85.9
Msa0180390 AT1G63580.1 29.469 207 139 5 49 250 35 239 2.70e-19 85.1
Msa0180390 AT3G22040.1 27.907 258 159 9 4 248 7 250 3.28e-19 84.7
Msa0180390 AT1G63570.3 26.800 250 168 5 3 249 4 241 2.54e-18 82.8
Msa0180390 AT1G63570.1 26.800 250 168 5 3 249 4 241 2.54e-18 82.8
Msa0180390 AT5G41300.1 27.126 247 167 6 6 247 7 245 3.20e-18 82.4
Msa0180390 AT1G63570.2 26.800 250 168 5 3 249 57 294 3.41e-18 83.2
Msa0180390 AT4G11470.2 26.168 214 143 6 51 254 43 251 5.18e-18 84.0
Msa0180390 AT4G11470.1 26.168 214 143 6 51 254 38 246 5.63e-18 83.6
Msa0180390 AT4G11480.2 24.257 202 150 3 50 250 37 236 9.36e-18 82.8
Msa0180390 AT4G11480.1 24.257 202 150 3 50 250 37 236 1.00e-17 83.2
Msa0180390 AT4G11480.3 24.257 202 150 3 50 250 37 236 1.12e-17 82.8
Msa0180390 AT4G11480.4 24.257 202 150 3 50 250 37 236 1.23e-17 82.8
Msa0180390 AT4G11460.2 31.034 174 105 5 87 248 1 171 1.79e-17 82.4
Msa0180390 AT3G29050.2 27.372 274 175 9 4 258 10 278 1.91e-17 80.5
Msa0180390 AT4G23190.3 30.636 173 100 5 89 248 1 166 8.09e-17 80.1
Msa0180390 AT4G23190.4 30.636 173 100 5 89 248 1 166 8.09e-17 80.1
Msa0180390 AT5G41280.1 27.530 247 161 7 12 249 7 244 8.42e-17 78.6
Msa0180390 AT3G21940.1 27.636 275 166 10 2 255 12 274 1.57e-16 77.8
Msa0180390 AT3G21940.2 30.556 180 106 4 92 255 3 179 1.63e-16 75.9
Msa0180390 AT3G22010.1 27.928 222 132 9 42 248 48 256 9.07e-16 75.5
Msa0180390 AT4G20680.1 28.342 187 123 5 71 248 76 260 4.15e-15 73.6
Msa0180390 AT4G23290.2 28.986 207 133 7 51 248 41 242 6.79e-15 74.7
Msa0180390 AT3G21900.2 25.481 208 146 4 41 248 45 243 1.17e-14 72.0
Msa0180390 AT3G22050.1 26.941 219 134 6 43 248 46 251 1.86e-14 71.6
Msa0180390 AT4G04570.1 27.179 195 132 4 68 254 59 251 2.35e-14 73.2
Msa0180390 AT4G04570.2 27.179 195 132 4 68 254 59 251 2.58e-14 72.8
Msa0180390 AT1G63590.1 25.000 192 123 4 71 250 64 246 1.02e-13 69.7
Msa0180390 AT3G22000.1 25.498 251 172 8 8 248 15 260 1.38e-13 69.3
Msa0180390 AT4G04510.2 28.283 198 119 6 71 254 62 250 2.69e-13 69.7
Msa0180390 AT4G04510.1 28.283 198 119 6 71 254 60 248 2.93e-13 69.7
Msa0180390 AT1G70690.1 26.829 205 126 7 49 248 48 233 5.23e-13 68.2
Msa0180390 AT3G21970.1 26.786 224 144 7 39 248 40 257 1.78e-12 66.2
Msa0180390 AT4G04490.1 26.126 222 131 7 50 253 40 246 2.68e-12 67.0
Msa0180390 AT4G04540.4 23.111 225 158 6 43 254 32 254 2.77e-12 67.0
Msa0180390 AT4G04540.1 23.111 225 158 6 43 254 32 254 3.65e-12 66.6
Msa0180390 AT4G04540.3 23.111 225 158 6 43 254 32 254 3.79e-12 66.2
Msa0180390 AT4G04540.2 23.111 225 158 6 43 254 32 254 3.83e-12 66.2
Msa0180390 AT2G01660.2 24.206 252 153 11 10 249 6 231 7.85e-12 64.3
Msa0180390 AT2G01660.1 24.400 250 151 11 10 247 6 229 1.06e-11 64.3
Msa0180390 AT2G01660.3 24.400 250 151 11 10 247 6 229 1.13e-11 63.9
Msa0180390 AT3G21900.1 24.038 208 141 4 41 248 45 235 2.92e-11 62.4

Find 48 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTAGGCCTAAACGAAAT+TGG 0.229067 1_4:-68218986 Msa0180390:CDS
TATTCAGACCGTTCTATATT+AGG 0.252445 1_4:-68219001 Msa0180390:CDS
AGTTACCTCTATCGTTATTT+TGG 0.283454 1_4:+68219287 None:intergenic
GGCAGATCAATTTAATATCT+TGG 0.285791 1_4:-68218924 Msa0180390:CDS
CGTTTACTTTGTCATTGTTT+TGG 0.294790 1_4:+68219164 None:intergenic
TTTCAAGATGTTGTAGCAAT+AGG 0.335675 1_4:-68218721 Msa0180390:CDS
TTGCTTTAGGTTGATAGAAT+TGG 0.343077 1_4:+68218645 None:intergenic
TTAAGAAGCAAGGCTATAAA+TGG 0.352413 1_4:-68218881 Msa0180390:CDS
TTGGTCGTTGTTGTCTGCTT+GGG 0.365814 1_4:+68219330 None:intergenic
AAGCAGGTCCAATTTCGTTT+AGG 0.394951 1_4:+68218978 None:intergenic
AGTAGTAATCAAATAATATA+TGG 0.412356 1_4:-68218815 Msa0180390:CDS
TTAGACCAAGCTGTAATTTG+AGG 0.412757 1_4:-68218682 Msa0180390:CDS
TTTGGTCGTTGTTGTCTGCT+TGG 0.426348 1_4:+68219329 None:intergenic
GATAATTATGAGAAAGAGAA+AGG 0.435798 1_4:+68219369 None:intergenic
CAGTGTACACCTGACTTGTC+TGG 0.440744 1_4:-68218785 Msa0180390:CDS
TGCTTGTGTGAGATTGAATC+TGG 0.442950 1_4:+68219081 None:intergenic
AATGACAAAGTAAACGTGAT+TGG 0.443303 1_4:-68219157 Msa0180390:CDS
AGGCATTTGCGACAATCATC+AGG 0.449621 1_4:+68219112 None:intergenic
TCACATCGTAAGTATGTTGT+AGG 0.450527 1_4:-68218854 Msa0180390:CDS
TGCGACCAAAATAACGATAG+AGG 0.460057 1_4:-68219292 Msa0180390:CDS
CTTGGGGTTTAGATGTGAAA+AGG 0.460200 1_4:+68219347 None:intergenic
TATATTATTTGATTACTACT+AGG 0.467816 1_4:+68218818 None:intergenic
GATAGAGGTAACTACATAAC+CGG 0.483653 1_4:-68219277 Msa0180390:CDS
TGAGAAAGAGAAAGGAGAGT+GGG 0.486566 1_4:+68219377 None:intergenic
GTTGAATGATTTAAGAAGCA+AGG 0.524724 1_4:-68218891 Msa0180390:CDS
TCAAACCTCAAATTACAGCT+TGG 0.526400 1_4:+68218677 None:intergenic
AGATGTTGTAGCAATAGGAT+TGG 0.527777 1_4:-68218716 Msa0180390:CDS
CACTGGGGACCAGACAAGTC+AGG 0.531473 1_4:+68218776 None:intergenic
GTATGTTGTAGGAACAATGC+CGG 0.534380 1_4:-68218843 Msa0180390:CDS
GATTGTCGCAAATGCCTAGT+TGG 0.540455 1_4:-68219106 Msa0180390:CDS
ATGAGAAAGAGAAAGGAGAG+TGG 0.541993 1_4:+68219376 None:intergenic
GCTGTTGTGGTAGGTATCAC+CGG 0.542628 1_4:+68219258 None:intergenic
GAGCAATTCTGAGGCTGTTG+TGG 0.544607 1_4:+68219245 None:intergenic
AAGTTCCATGCAAAATAAGC+AGG 0.549615 1_4:+68218962 None:intergenic
CGTTTAGGCCTAATATAGAA+CGG 0.560797 1_4:+68218993 None:intergenic
GAACTTGAACAATGCAACAA+TGG 0.575248 1_4:-68218945 Msa0180390:CDS
GATTGAATCTGGAACCAACT+AGG 0.575788 1_4:+68219092 None:intergenic
TGGTCGTTGTTGTCTGCTTG+GGG 0.575882 1_4:+68219331 None:intergenic
AATTCTGAGGCTGTTGTGGT+AGG 0.584609 1_4:+68219249 None:intergenic
AAGCAAACAATCATCACACT+GGG 0.585932 1_4:+68218760 None:intergenic
GAAGCAAACAATCATCACAC+TGG 0.591047 1_4:+68218759 None:intergenic
TATGTTGTAGGAACAATGCC+GGG 0.598716 1_4:-68218842 Msa0180390:CDS
GGTTTCTACAACACCAACAA+TGG 0.608559 1_4:-68219187 Msa0180390:CDS
AACGTGATTGGAATATGTAG+AGG 0.617417 1_4:-68219145 Msa0180390:CDS
ATTTGATTACTACTAGGACC+CGG 0.639406 1_4:+68218824 None:intergenic
AGAAGCAATTGGATGGTACG+AGG 0.670531 1_4:-68219044 Msa0180390:CDS
GGGAGATAAGAGCAATTCTG+AGG 0.672639 1_4:+68219236 None:intergenic
AGCAAACAATCATCACACTG+GGG 0.800516 1_4:+68218761 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTATTTGATTACTACT+AGG + chr1_4:68219204-68219223 None:intergenic 15.0%
!! AGTAGTAATCAAATAATATA+TGG - chr1_4:68219204-68219223 Msa0180390:CDS 15.0%
!! TATATTATTTGATTACTACT+AGG + chr1_4:68219204-68219223 None:intergenic 15.0%
!! AGTAGTAATCAAATAATATA+TGG - chr1_4:68219204-68219223 Msa0180390:CDS 15.0%
! GATAATTATGAGAAAGAGAA+AGG + chr1_4:68218653-68218672 None:intergenic 25.0%
!!! AATTTTTGAGTTGTAAGTGA+GGG + chr1_4:68218806-68218825 None:intergenic 25.0%
! TACAACTCAAAAATTGACTA+CGG - chr1_4:68218811-68218830 Msa0180390:CDS 25.0%
!!! CCAATTGCTTCTTTTTTATT+TGG + chr1_4:68218967-68218986 None:intergenic 25.0%
! CCAAATAAAAAAGAAGCAAT+TGG - chr1_4:68218964-68218983 Msa0180390:CDS 25.0%
! ATAAAAAAGAAGCAATTGGA+TGG - chr1_4:68218968-68218987 Msa0180390:CDS 25.0%
! GATAATTATGAGAAAGAGAA+AGG + chr1_4:68218653-68218672 None:intergenic 25.0%
!!! AATTTTTGAGTTGTAAGTGA+GGG + chr1_4:68218806-68218825 None:intergenic 25.0%
! TACAACTCAAAAATTGACTA+CGG - chr1_4:68218811-68218830 Msa0180390:CDS 25.0%
!!! CCAATTGCTTCTTTTTTATT+TGG + chr1_4:68218967-68218986 None:intergenic 25.0%
! CCAAATAAAAAAGAAGCAAT+TGG - chr1_4:68218964-68218983 Msa0180390:CDS 25.0%
! ATAAAAAAGAAGCAATTGGA+TGG - chr1_4:68218968-68218987 Msa0180390:CDS 25.0%
! AGTTACCTCTATCGTTATTT+TGG + chr1_4:68218735-68218754 None:intergenic 30.0%
!!! CAATTTTTGAGTTGTAAGTG+AGG + chr1_4:68218807-68218826 None:intergenic 30.0%
! CGTTTACTTTGTCATTGTTT+TGG + chr1_4:68218858-68218877 None:intergenic 30.0%
AATGACAAAGTAAACGTGAT+TGG - chr1_4:68218862-68218881 Msa0180390:CDS 30.0%
TATTCAGACCGTTCTATATT+AGG - chr1_4:68219018-68219037 Msa0180390:CDS 30.0%
ATATTAGGCCTAAACGAAAT+TGG - chr1_4:68219033-68219052 Msa0180390:CDS 30.0%
GGCAGATCAATTTAATATCT+TGG - chr1_4:68219095-68219114 Msa0180390:CDS 30.0%
GTTGAATGATTTAAGAAGCA+AGG - chr1_4:68219128-68219147 Msa0180390:CDS 30.0%
TTAAGAAGCAAGGCTATAAA+TGG - chr1_4:68219138-68219157 Msa0180390:CDS 30.0%
TTTCAAGATGTTGTAGCAAT+AGG - chr1_4:68219298-68219317 Msa0180390:CDS 30.0%
!! TTGCTTTAGGTTGATAGAAT+TGG + chr1_4:68219377-68219396 None:intergenic 30.0%
! AGTTACCTCTATCGTTATTT+TGG + chr1_4:68218735-68218754 None:intergenic 30.0%
!!! CAATTTTTGAGTTGTAAGTG+AGG + chr1_4:68218807-68218826 None:intergenic 30.0%
! CGTTTACTTTGTCATTGTTT+TGG + chr1_4:68218858-68218877 None:intergenic 30.0%
AATGACAAAGTAAACGTGAT+TGG - chr1_4:68218862-68218881 Msa0180390:CDS 30.0%
TATTCAGACCGTTCTATATT+AGG - chr1_4:68219018-68219037 Msa0180390:CDS 30.0%
ATATTAGGCCTAAACGAAAT+TGG - chr1_4:68219033-68219052 Msa0180390:CDS 30.0%
GGCAGATCAATTTAATATCT+TGG - chr1_4:68219095-68219114 Msa0180390:CDS 30.0%
GTTGAATGATTTAAGAAGCA+AGG - chr1_4:68219128-68219147 Msa0180390:CDS 30.0%
TTAAGAAGCAAGGCTATAAA+TGG - chr1_4:68219138-68219157 Msa0180390:CDS 30.0%
TTTCAAGATGTTGTAGCAAT+AGG - chr1_4:68219298-68219317 Msa0180390:CDS 30.0%
!! TTGCTTTAGGTTGATAGAAT+TGG + chr1_4:68219377-68219396 None:intergenic 30.0%
!! CGCAAAAGTAGTGGAATTTT+TGG + chr1_4:68218711-68218730 None:intergenic 35.0%
!!! TATTTTGGTCGCAAAAGTAG+TGG + chr1_4:68218720-68218739 None:intergenic 35.0%
GATAGAGGTAACTACATAAC+CGG - chr1_4:68218742-68218761 Msa0180390:CDS 35.0%
AACGTGATTGGAATATGTAG+AGG - chr1_4:68218874-68218893 Msa0180390:CDS 35.0%
CGTTTAGGCCTAATATAGAA+CGG + chr1_4:68219029-68219048 None:intergenic 35.0%
AAGTTCCATGCAAAATAAGC+AGG + chr1_4:68219060-68219079 None:intergenic 35.0%
GAACTTGAACAATGCAACAA+TGG - chr1_4:68219074-68219093 Msa0180390:CDS 35.0%
TCACATCGTAAGTATGTTGT+AGG - chr1_4:68219165-68219184 Msa0180390:CDS 35.0%
ATTTGATTACTACTAGGACC+CGG + chr1_4:68219198-68219217 None:intergenic 35.0%
AAGCAAACAATCATCACACT+GGG + chr1_4:68219262-68219281 None:intergenic 35.0%
AGATGTTGTAGCAATAGGAT+TGG - chr1_4:68219303-68219322 Msa0180390:CDS 35.0%
TTAGACCAAGCTGTAATTTG+AGG - chr1_4:68219337-68219356 Msa0180390:CDS 35.0%
TCAAACCTCAAATTACAGCT+TGG + chr1_4:68219345-68219364 None:intergenic 35.0%
! TGTGAAGCAAGTTTTGCTTT+AGG + chr1_4:68219390-68219409 None:intergenic 35.0%
!! CGCAAAAGTAGTGGAATTTT+TGG + chr1_4:68218711-68218730 None:intergenic 35.0%
!!! TATTTTGGTCGCAAAAGTAG+TGG + chr1_4:68218720-68218739 None:intergenic 35.0%
GATAGAGGTAACTACATAAC+CGG - chr1_4:68218742-68218761 Msa0180390:CDS 35.0%
AACGTGATTGGAATATGTAG+AGG - chr1_4:68218874-68218893 Msa0180390:CDS 35.0%
CGTTTAGGCCTAATATAGAA+CGG + chr1_4:68219029-68219048 None:intergenic 35.0%
AAGTTCCATGCAAAATAAGC+AGG + chr1_4:68219060-68219079 None:intergenic 35.0%
GAACTTGAACAATGCAACAA+TGG - chr1_4:68219074-68219093 Msa0180390:CDS 35.0%
TCACATCGTAAGTATGTTGT+AGG - chr1_4:68219165-68219184 Msa0180390:CDS 35.0%
ATTTGATTACTACTAGGACC+CGG + chr1_4:68219198-68219217 None:intergenic 35.0%
AAGCAAACAATCATCACACT+GGG + chr1_4:68219262-68219281 None:intergenic 35.0%
AGATGTTGTAGCAATAGGAT+TGG - chr1_4:68219303-68219322 Msa0180390:CDS 35.0%
TTAGACCAAGCTGTAATTTG+AGG - chr1_4:68219337-68219356 Msa0180390:CDS 35.0%
TCAAACCTCAAATTACAGCT+TGG + chr1_4:68219345-68219364 None:intergenic 35.0%
! TGTGAAGCAAGTTTTGCTTT+AGG + chr1_4:68219390-68219409 None:intergenic 35.0%
TGAGAAAGAGAAAGGAGAGT+GGG + chr1_4:68218645-68218664 None:intergenic 40.0%
ATGAGAAAGAGAAAGGAGAG+TGG + chr1_4:68218646-68218665 None:intergenic 40.0%
CTTGGGGTTTAGATGTGAAA+AGG + chr1_4:68218675-68218694 None:intergenic 40.0%
TGCGACCAAAATAACGATAG+AGG - chr1_4:68218727-68218746 Msa0180390:CDS 40.0%
GGTTTCTACAACACCAACAA+TGG - chr1_4:68218832-68218851 Msa0180390:CDS 40.0%
!!! GTCATTGTTTTGGCCATTGT+TGG + chr1_4:68218848-68218867 None:intergenic 40.0%
! GATTGAATCTGGAACCAACT+AGG + chr1_4:68218930-68218949 None:intergenic 40.0%
TGCTTGTGTGAGATTGAATC+TGG + chr1_4:68218941-68218960 None:intergenic 40.0%
AAGCAGGTCCAATTTCGTTT+AGG + chr1_4:68219044-68219063 None:intergenic 40.0%
!!! TTGGACCTGCTTATTTTGCA+TGG - chr1_4:68219052-68219071 Msa0180390:CDS 40.0%
GTATGTTGTAGGAACAATGC+CGG - chr1_4:68219176-68219195 Msa0180390:CDS 40.0%
TATGTTGTAGGAACAATGCC+GGG - chr1_4:68219177-68219196 Msa0180390:CDS 40.0%
AGCAAACAATCATCACACTG+GGG + chr1_4:68219261-68219280 None:intergenic 40.0%
GAAGCAAACAATCATCACAC+TGG + chr1_4:68219263-68219282 None:intergenic 40.0%
TGAGAAAGAGAAAGGAGAGT+GGG + chr1_4:68218645-68218664 None:intergenic 40.0%
ATGAGAAAGAGAAAGGAGAG+TGG + chr1_4:68218646-68218665 None:intergenic 40.0%
CTTGGGGTTTAGATGTGAAA+AGG + chr1_4:68218675-68218694 None:intergenic 40.0%
TGCGACCAAAATAACGATAG+AGG - chr1_4:68218727-68218746 Msa0180390:CDS 40.0%
GGTTTCTACAACACCAACAA+TGG - chr1_4:68218832-68218851 Msa0180390:CDS 40.0%
!!! GTCATTGTTTTGGCCATTGT+TGG + chr1_4:68218848-68218867 None:intergenic 40.0%
! GATTGAATCTGGAACCAACT+AGG + chr1_4:68218930-68218949 None:intergenic 40.0%
TGCTTGTGTGAGATTGAATC+TGG + chr1_4:68218941-68218960 None:intergenic 40.0%
AAGCAGGTCCAATTTCGTTT+AGG + chr1_4:68219044-68219063 None:intergenic 40.0%
!!! TTGGACCTGCTTATTTTGCA+TGG - chr1_4:68219052-68219071 Msa0180390:CDS 40.0%
GTATGTTGTAGGAACAATGC+CGG - chr1_4:68219176-68219195 Msa0180390:CDS 40.0%
TATGTTGTAGGAACAATGCC+GGG - chr1_4:68219177-68219196 Msa0180390:CDS 40.0%
AGCAAACAATCATCACACTG+GGG + chr1_4:68219261-68219280 None:intergenic 40.0%
GAAGCAAACAATCATCACAC+TGG + chr1_4:68219263-68219282 None:intergenic 40.0%
TTGGTCGTTGTTGTCTGCTT+GGG + chr1_4:68218692-68218711 None:intergenic 45.0%
TTTGGTCGTTGTTGTCTGCT+TGG + chr1_4:68218693-68218712 None:intergenic 45.0%
!! AATTCTGAGGCTGTTGTGGT+AGG + chr1_4:68218773-68218792 None:intergenic 45.0%
GGGAGATAAGAGCAATTCTG+AGG + chr1_4:68218786-68218805 None:intergenic 45.0%
AGGCATTTGCGACAATCATC+AGG + chr1_4:68218910-68218929 None:intergenic 45.0%
GATTGTCGCAAATGCCTAGT+TGG - chr1_4:68218913-68218932 Msa0180390:CDS 45.0%
AGAAGCAATTGGATGGTACG+AGG - chr1_4:68218975-68218994 Msa0180390:CDS 45.0%
TTGGTCGTTGTTGTCTGCTT+GGG + chr1_4:68218692-68218711 None:intergenic 45.0%
TTTGGTCGTTGTTGTCTGCT+TGG + chr1_4:68218693-68218712 None:intergenic 45.0%
!! AATTCTGAGGCTGTTGTGGT+AGG + chr1_4:68218773-68218792 None:intergenic 45.0%
GGGAGATAAGAGCAATTCTG+AGG + chr1_4:68218786-68218805 None:intergenic 45.0%
AGGCATTTGCGACAATCATC+AGG + chr1_4:68218910-68218929 None:intergenic 45.0%
GATTGTCGCAAATGCCTAGT+TGG - chr1_4:68218913-68218932 Msa0180390:CDS 45.0%
AGAAGCAATTGGATGGTACG+AGG - chr1_4:68218975-68218994 Msa0180390:CDS 45.0%
TGGTCGTTGTTGTCTGCTTG+GGG + chr1_4:68218691-68218710 None:intergenic 50.0%
GCTGTTGTGGTAGGTATCAC+CGG + chr1_4:68218764-68218783 None:intergenic 50.0%
!! GAGCAATTCTGAGGCTGTTG+TGG + chr1_4:68218777-68218796 None:intergenic 50.0%
! CAGTGTACACCTGACTTGTC+TGG - chr1_4:68219234-68219253 Msa0180390:CDS 50.0%
TGGTCGTTGTTGTCTGCTTG+GGG + chr1_4:68218691-68218710 None:intergenic 50.0%
GCTGTTGTGGTAGGTATCAC+CGG + chr1_4:68218764-68218783 None:intergenic 50.0%
!! GAGCAATTCTGAGGCTGTTG+TGG + chr1_4:68218777-68218796 None:intergenic 50.0%
! CAGTGTACACCTGACTTGTC+TGG - chr1_4:68219234-68219253 Msa0180390:CDS 50.0%
CACTGGGGACCAGACAAGTC+AGG + chr1_4:68219246-68219265 None:intergenic 60.0%
CACTGGGGACCAGACAAGTC+AGG + chr1_4:68219246-68219265 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 68218622 68219419 68218622 ID=Msa0180390;Name=Msa0180390
chr1_4 mRNA 68218622 68219419 68218622 ID=Msa0180390-mRNA-1;Parent=Msa0180390;Name=Msa0180390-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|-1|0|1|-1|1|1|0|265
chr1_4 exon 68218622 68219419 68218622 ID=Msa0180390-mRNA-1:exon:22553;Parent=Msa0180390-mRNA-1
chr1_4 CDS 68218622 68219419 68218622 ID=Msa0180390-mRNA-1:cds;Parent=Msa0180390-mRNA-1
Gene Sequence

>Msa0180390

ATGTCTATTCTCTCTTTAAGCCCACTCTCCTTTCTCTTTCTCATAATTATCCTTTTCACATCTAAACCCCAAGCAGACAACAACGACCAAAAATTCCACTACTTTTGCGACCAAAATAACGATAGAGGTAACTACATAACCGGTGATACCTACCACAACAGCCTCAGAATTGCTCTTATCTCCCTCACTTACAACTCAAAAATTGACTACGGTTTCTACAACACCAACAATGGCCAAAACAATGACAAAGTAAACGTGATTGGAATATGTAGAGGAGACATTAATCCTGATGATTGTCGCAAATGCCTAGTTGGTTCCAGATTCAATCTCACACAAGCATGTCCAAATAAAAAAGAAGCAATTGGATGGTACGAGGATGAGAAATGCATGTTACGTTATTCAGACCGTTCTATATTAGGCCTAAACGAAATTGGACCTGCTTATTTTGCATGGAACTTGAACAATGCAACAATGGCAGATCAATTTAATATCTTGGTAAAACAACTGTTGAATGATTTAAGAAGCAAGGCTATAAATGGTGATTCACATCGTAAGTATGTTGTAGGAACAATGCCGGGTCCTAGTAGTAATCAAATAATATATGGTCTTGTTCAGTGTACACCTGACTTGTCTGGTCCCCAGTGTGATGATTGTTTGCTTCAATCTATTGCTGAAGTTTCAAGATGTTGTAGCAATAGGATTGGTGCTAGAATTGTTAGACCAAGCTGTAATTTGAGGTTTGAAACTTCATACCAATTCTATCAACCTAAAGCAAAACTTGCTTCACATCTCCCATGA
Protein sequence

>Msa0180390

MSILSLSPLSFLFLIIILFTSKPQADNNDQKFHYFCDQNNDRGNYITGDTYHNSLRIALISLTYNSKIDYGFYNTNNGQNNDKVNVIGICRGDINPDDCRKCLVGSRFNLTQACPNKKEAIGWYEDEKCMLRYSDRSILGLNEIGPAYFAWNLNNATMADQFNILVKQLLNDLRSKAINGDSHRKYVVGTMPGPSSNQIIYGLVQCTPDLSGPQCDDCLLQSIAEVSRCCSNRIGARIVRPSCNLRFETSYQFYQPKAKLASHLP*