AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0135740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135740 MtrunA17_Chr1g0206601 91.321 265 23 0 1 265 22 286 0.0 511
Msa0135740 MtrunA17_Chr1g0206581 81.022 274 42 2 1 265 18 290 7.54e-162 450
Msa0135740 MtrunA17_Chr1g0206651 72.656 256 64 4 1 255 1 251 7.51e-134 378
Msa0135740 MtrunA17_Chr1g0206571 62.343 239 90 0 20 258 23 261 1.44e-108 316
Msa0135740 MtrunA17_Chr1g0154361 61.753 251 91 3 10 255 3 253 7.45e-106 309
Msa0135740 MtrunA17_Chr1g0154341 56.923 260 107 3 10 264 3 262 2.08e-100 294
Msa0135740 MtrunA17_Chr1g0154371 55.769 260 108 4 1 254 8 266 4.67e-93 288
Msa0135740 MtrunA17_Chr1g0154311 54.435 248 109 2 14 258 10 256 6.81e-92 285
Msa0135740 MtrunA17_Chr1g0154351 55.952 252 106 3 10 256 3 254 3.98e-91 283
Msa0135740 MtrunA17_Chr1g0154321 54.902 255 106 5 10 255 3 257 9.01e-87 274
Msa0135740 MtrunA17_Chr1g0206531 51.373 255 112 6 4 256 8 252 1.24e-79 253
Msa0135740 MtrunA17_Chr5g0428591 49.187 246 117 5 10 255 8 245 3.73e-73 225
Msa0135740 MtrunA17_Chr1g0206461 48.235 255 121 5 2 256 6 249 7.23e-72 232
Msa0135740 MtrunA17_Chr5g0428571 51.364 220 103 3 40 259 35 250 3.86e-67 217
Msa0135740 MtrunA17_Chr5g0428531 46.063 254 114 7 10 254 8 247 1.40e-65 206
Msa0135740 MtrunA17_Chr1g0206451 49.219 256 121 5 2 256 40 287 1.47e-63 211
Msa0135740 MtrunA17_Chr1g0206521 47.843 255 123 5 2 256 648 892 8.83e-62 210
Msa0135740 MtrunA17_Chr1g0206521 47.718 241 117 5 16 256 19 250 1.18e-59 204
Msa0135740 MtrunA17_Chr1g0206561 40.873 252 140 4 11 262 4 246 6.44e-52 179
Msa0135740 MtrunA17_Chr5g0426941 41.288 264 134 8 4 262 1 248 4.25e-51 177
Msa0135740 MtrunA17_Chr1g0206381 37.917 240 135 6 24 255 19 252 8.63e-44 151
Msa0135740 MtrunA17_Chr1g0206401 43.256 215 107 8 43 248 33 241 2.27e-43 156
Msa0135740 MtrunA17_Chr2g0323011 31.452 248 158 7 4 248 1 239 1.98e-34 124
Msa0135740 MtrunA17_Chr1g0205771 33.617 235 145 6 24 255 33 259 1.62e-32 125
Msa0135740 MtrunA17_Chr1g0206331 34.562 217 133 7 44 255 41 253 6.17e-32 124
Msa0135740 MtrunA17_Chr5g0428601 48.413 126 62 3 130 255 1 123 2.04e-31 119
Msa0135740 MtrunA17_Chr7g0234231 29.839 248 165 5 9 256 1 239 1.39e-30 114
Msa0135740 MtrunA17_Chr6g0475001 33.730 252 153 8 11 255 7 251 1.82e-30 119
Msa0135740 MtrunA17_Chr6g0475001 34.184 196 113 8 68 254 301 489 9.40e-18 82.8
Msa0135740 MtrunA17_Chr6g0475121 32.046 259 162 7 9 261 7 257 6.68e-29 114
Msa0135740 MtrunA17_Chr6g0475121 28.992 238 150 10 21 254 268 490 1.27e-18 85.1
Msa0135740 MtrunA17_Chr6g0474901 31.776 214 137 5 47 255 35 244 1.95e-28 114
Msa0135740 MtrunA17_Chr6g0474901 30.864 243 142 11 23 254 256 483 3.03e-19 87.0
Msa0135740 MtrunA17_Chr6g0474971 33.178 214 134 5 47 255 35 244 3.33e-28 109
Msa0135740 MtrunA17_Chr5g0394001 32.114 246 155 8 13 254 12 249 3.46e-28 113
Msa0135740 MtrunA17_Chr1g0206201 32.271 251 156 9 4 248 6 248 3.29e-27 106
Msa0135740 MtrunA17_Chr1g0206361 32.735 223 139 7 40 258 42 257 4.42e-27 109
Msa0135740 MtrunA17_Chr8g0347571 29.818 275 160 8 9 263 7 268 1.45e-26 108
Msa0135740 MtrunA17_Chr8g0347571 27.626 257 154 13 23 265 261 499 1.09e-17 82.4
Msa0135740 MtrunA17_Chr6g0475201 30.303 264 173 6 1 261 1 256 5.43e-26 107
Msa0135740 MtrunA17_Chr6g0475201 35.714 168 101 6 89 254 328 490 3.38e-23 98.6
Msa0135740 MtrunA17_Chr6g0474811 31.203 266 155 10 4 255 1 252 6.72e-26 106
Msa0135740 MtrunA17_Chr6g0474811 32.500 200 122 7 68 259 303 497 5.50e-20 89.4
Msa0135740 MtrunA17_Chr6g0475111 29.719 249 166 6 9 254 10 252 1.29e-25 105
Msa0135740 MtrunA17_Chr6g0475111 30.058 173 108 7 89 255 337 502 5.58e-17 80.5
Msa0135740 MtrunA17_Chr1g0206351 29.870 231 152 5 28 255 31 254 1.34e-25 105
Msa0135740 MtrunA17_Chr6g0474871 31.076 251 158 8 12 255 7 249 2.31e-25 105
Msa0135740 MtrunA17_Chr6g0474871 31.746 189 116 7 74 254 304 487 1.71e-18 85.1
Msa0135740 MtrunA17_Chr2g0322981 29.796 245 154 8 6 248 11 239 8.67e-25 99.0
Msa0135740 MtrunA17_Chr5g0394021 33.880 183 116 3 72 254 70 247 1.34e-24 102
Msa0135740 MtrunA17_Chr5g0393981 31.193 218 129 9 45 254 113 317 1.99e-24 102
Msa0135740 MtrunA17_Chr3g0081001 27.341 267 175 6 3 265 5 256 3.87e-24 101
Msa0135740 MtrunA17_Chr1g0206231 32.536 209 131 7 51 255 52 254 9.24e-24 100
Msa0135740 MtrunA17_Chr1g0206291 30.435 253 158 9 11 255 23 265 1.97e-23 99.0
Msa0135740 MtrunA17_Chr6g0475091 31.797 217 139 5 44 255 48 260 2.00e-23 99.4
Msa0135740 MtrunA17_Chr6g0475091 33.889 180 112 6 82 259 330 504 5.58e-18 83.6
Msa0135740 MtrunA17_Chr6g0474861 32.932 249 150 11 11 254 7 243 2.36e-23 99.0
Msa0135740 MtrunA17_Chr3g0081051 32.547 212 134 5 41 249 31 236 3.08e-23 95.1
Msa0135740 MtrunA17_Chr5g0394011 31.019 216 138 7 45 254 41 251 3.27e-23 98.6
Msa0135740 MtrunA17_Chr3g0081011 27.650 217 149 5 50 265 46 255 4.12e-23 98.2
Msa0135740 MtrunA17_Chr3g0081031 31.967 244 152 6 9 249 22 254 1.18e-22 97.1
Msa0135740 MtrunA17_Chr8g0354271 27.027 259 178 7 9 264 8 258 3.54e-21 92.8
Msa0135740 MtrunA17_Chr8g0354421 30.350 257 153 13 9 255 30 270 3.55e-20 89.7
Msa0135740 MtrunA17_Chr8g0354441 27.407 270 167 12 9 265 13 266 7.57e-20 89.0
Msa0135740 MtrunA17_Chr8g0354351 26.154 260 176 8 9 265 7 253 1.80e-19 87.8
Msa0135740 MtrunA17_Chr8g0354411 27.979 193 129 6 75 265 74 258 2.58e-18 82.8
Msa0135740 MtrunA17_Chr8g0354341 31.694 183 114 6 75 255 73 246 1.97e-17 79.7
Msa0135740 MtrunA17_Chr3g0119041 29.665 209 126 7 68 259 63 267 2.51e-17 81.3
Msa0135740 MtrunA17_Chr1g0206251 34.254 181 107 7 82 254 333 509 2.52e-17 81.3
Msa0135740 MtrunA17_Chr1g0206251 27.734 256 167 9 11 256 19 266 2.08e-13 69.7
Msa0135740 MtrunA17_Chr1g0206421 30.189 159 94 5 71 220 2 152 6.78e-17 80.1
Msa0135740 MtrunA17_Chr2g0280361 27.197 239 157 7 25 262 27 249 1.73e-16 77.0
Msa0135740 MtrunA17_Chr2g0280391 25.941 239 165 7 25 262 27 254 1.97e-16 77.0
Msa0135740 MtrunA17_Chr1g0206411 53.448 58 27 0 65 122 4 61 6.38e-15 68.6
Msa0135740 MtrunA17_Chr8g0354431 23.938 259 187 6 9 265 8 258 1.16e-12 67.4
Msa0135740 MtrunA17_Chr8g0354281 22.368 228 163 8 32 255 30 247 3.11e-12 66.2
Msa0135740 MtrunA17_Chr2g0280381 25.837 209 143 5 55 262 51 248 1.29e-11 63.2
Msa0135740 MtrunA17_Chr1g0206541 44.643 56 30 1 208 263 19 73 5.47e-11 58.2
Msa0135740 MtrunA17_Chr3g0082951 33.613 119 75 3 22 139 106 221 8.64e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135740 AT4G21410.2 36.719 256 152 6 13 265 13 261 1.06e-42 152
Msa0135740 AT4G21410.3 36.719 256 152 6 13 265 13 261 3.46e-42 153
Msa0135740 AT4G38830.1 37.273 220 132 5 39 256 32 247 2.83e-37 139
Msa0135740 AT4G05200.1 36.145 249 139 9 11 254 15 248 1.40e-36 137
Msa0135740 AT4G05200.2 36.145 249 139 9 11 254 33 266 1.44e-36 137
Msa0135740 AT4G21400.3 34.667 225 142 4 43 265 42 263 2.95e-36 135
Msa0135740 AT4G21400.6 34.667 225 142 4 43 265 42 263 3.78e-36 135
Msa0135740 AT4G21400.4 34.667 225 142 4 43 265 42 263 3.78e-36 135
Msa0135740 AT4G21400.5 34.667 225 142 4 43 265 42 263 3.78e-36 135
Msa0135740 AT4G21400.2 33.071 254 163 5 14 265 15 263 7.88e-36 135
Msa0135740 AT4G21400.1 33.071 254 163 5 14 265 15 263 9.18e-36 135
Msa0135740 AT3G22060.1 31.707 246 160 4 3 248 7 244 2.55e-35 127
Msa0135740 AT4G23170.1 38.426 216 118 8 45 254 39 245 3.57e-32 119
Msa0135740 AT4G21400.7 35.829 187 116 3 80 265 54 237 5.09e-32 124
Msa0135740 AT4G21230.1 35.897 234 125 9 41 263 37 256 1.50e-31 123
Msa0135740 AT4G00970.2 38.835 206 114 6 53 254 65 262 2.13e-31 122
Msa0135740 AT4G00970.1 38.835 206 114 6 53 254 65 262 3.67e-31 122
Msa0135740 AT4G23220.1 32.212 208 139 2 43 248 29 236 8.62e-31 120
Msa0135740 AT4G23160.3 37.220 223 127 7 41 258 632 846 2.66e-30 119
Msa0135740 AT4G23160.2 37.220 223 127 7 41 258 632 846 2.66e-30 119
Msa0135740 AT4G23180.3 36.752 234 132 9 29 258 36 257 2.73e-30 118
Msa0135740 AT4G23160.1 37.220 223 127 7 41 258 52 266 3.46e-30 119
Msa0135740 AT4G23180.2 36.752 234 132 9 29 258 36 257 5.04e-30 118
Msa0135740 AT4G23280.3 37.383 214 122 6 44 254 38 242 5.71e-30 118
Msa0135740 AT4G23180.1 36.752 234 132 9 29 258 36 257 5.96e-30 118
Msa0135740 AT4G23280.1 37.383 214 122 6 44 254 38 242 7.05e-30 118
Msa0135740 AT4G23280.2 37.383 214 122 6 44 254 38 242 7.30e-30 118
Msa0135740 AT4G23150.1 35.146 239 142 7 24 258 20 249 1.55e-29 117
Msa0135740 AT4G23130.3 35.577 208 123 7 51 255 46 245 3.91e-29 115
Msa0135740 AT4G23130.2 35.577 208 123 7 51 255 46 245 5.50e-29 115
Msa0135740 AT4G23130.1 35.577 208 123 7 51 255 46 245 5.79e-29 115
Msa0135740 AT4G23210.3 32.589 224 130 6 40 248 29 246 5.44e-28 112
Msa0135740 AT2G31620.1 30.942 223 141 6 40 256 40 255 5.57e-28 108
Msa0135740 AT4G23210.2 32.589 224 130 6 40 248 29 246 6.67e-28 112
Msa0135740 AT4G23210.4 32.589 224 130 6 40 248 29 246 6.67e-28 112
Msa0135740 AT4G23210.5 32.589 224 130 6 40 248 29 246 7.42e-28 112
Msa0135740 AT4G23200.2 29.524 210 142 2 45 248 30 239 7.76e-28 111
Msa0135740 AT4G23210.1 32.589 224 130 6 40 248 29 246 8.63e-28 112
Msa0135740 AT4G23300.1 32.735 223 129 6 41 248 30 246 9.05e-28 112
Msa0135740 AT4G23200.1 29.524 210 142 2 45 248 30 239 1.15e-27 112
Msa0135740 AT4G23140.2 35.176 199 115 6 65 258 61 250 8.07e-27 109
Msa0135740 AT4G23140.1 35.176 199 115 6 65 258 61 250 8.82e-27 109
Msa0135740 AT4G11521.1 28.889 225 147 3 32 248 18 237 5.00e-26 103
Msa0135740 AT4G11460.3 31.651 218 134 5 43 248 32 246 1.02e-25 106
Msa0135740 AT4G11460.1 31.651 218 134 5 43 248 32 246 1.04e-25 106
Msa0135740 AT3G45860.1 37.186 199 109 7 65 258 64 251 4.73e-25 104
Msa0135740 AT4G11530.1 26.506 249 163 4 9 248 5 242 6.53e-25 103
Msa0135740 AT5G41290.1 29.555 247 155 8 12 249 7 243 1.80e-24 99.8
Msa0135740 AT4G23230.1 34.135 208 124 7 51 254 43 241 3.27e-24 102
Msa0135740 AT3G21960.1 27.490 251 171 6 3 248 11 255 1.15e-23 97.1
Msa0135740 AT4G23270.2 32.540 252 152 10 7 254 3 240 1.33e-23 100
Msa0135740 AT4G23270.1 32.540 252 152 10 7 254 3 240 1.33e-23 100
Msa0135740 AT4G23270.3 32.540 252 152 10 7 254 3 240 1.39e-23 100
Msa0135740 AT4G23190.1 31.818 220 137 6 39 248 28 244 1.43e-23 100
Msa0135740 AT4G20640.1 28.112 249 168 5 5 248 7 249 1.57e-23 96.3
Msa0135740 AT4G20630.1 28.112 249 168 5 5 248 7 249 1.57e-23 96.3
Msa0135740 AT4G20580.1 28.112 249 168 5 5 248 7 249 1.57e-23 96.3
Msa0135740 AT4G20600.1 28.112 249 168 5 5 248 7 249 1.57e-23 96.3
Msa0135740 AT4G20620.1 28.112 249 168 5 5 248 7 249 1.57e-23 96.3
Msa0135740 AT4G20610.1 28.112 249 168 5 5 248 7 249 1.57e-23 96.3
Msa0135740 AT4G20590.1 28.112 249 168 5 5 248 7 249 1.57e-23 96.3
Msa0135740 AT4G20570.1 28.112 249 168 5 5 248 21 263 1.76e-23 96.7
Msa0135740 AT4G20560.1 28.112 249 168 5 5 248 21 263 1.76e-23 96.7
Msa0135740 AT4G20540.1 28.112 249 168 5 5 248 21 263 1.76e-23 96.7
Msa0135740 AT4G20645.1 28.112 249 168 5 5 248 21 263 1.76e-23 96.7
Msa0135740 AT4G20530.1 28.112 249 168 5 5 248 21 263 1.76e-23 96.7
Msa0135740 AT4G20550.1 28.112 249 168 5 5 248 21 263 1.76e-23 96.7
Msa0135740 AT4G23190.2 32.110 218 135 6 41 248 30 244 1.77e-23 99.8
Msa0135740 AT4G20670.1 28.112 249 168 5 5 248 7 249 2.12e-23 96.3
Msa0135740 AT3G58310.1 26.556 241 171 4 9 248 14 249 9.14e-23 94.4
Msa0135740 AT3G21990.1 27.309 249 170 5 5 248 7 249 1.45e-22 94.0
Msa0135740 AT1G61750.1 30.660 212 139 6 43 251 38 244 2.41e-22 94.7
Msa0135740 AT4G21410.1 40.580 138 78 3 130 265 1 136 2.54e-22 96.3
Msa0135740 AT4G23240.1 29.630 216 145 4 43 254 33 245 3.91e-22 95.9
Msa0135740 AT4G11490.1 29.954 217 144 3 38 248 27 241 6.22e-22 95.5
Msa0135740 AT4G23250.1 28.448 232 148 6 36 254 30 256 9.68e-22 94.7
Msa0135740 AT4G23250.3 28.448 232 148 6 36 254 30 256 9.96e-22 94.7
Msa0135740 AT4G23250.2 28.448 232 148 6 36 254 30 256 1.04e-21 94.7
Msa0135740 AT4G23250.2 27.358 212 129 5 43 254 733 919 1.22e-13 71.2
Msa0135740 AT4G23310.2 33.005 203 123 6 50 248 157 350 4.50e-21 92.8
Msa0135740 AT4G23310.1 33.005 203 123 6 50 248 157 350 4.78e-21 92.8
Msa0135740 AT5G48540.1 29.435 248 158 7 13 256 9 243 1.03e-20 89.0
Msa0135740 AT3G21910.1 28.634 227 148 7 42 258 48 270 1.08e-20 89.4
Msa0135740 AT4G21400.8 37.226 137 84 2 130 265 1 136 1.61e-20 91.3
Msa0135740 AT4G04500.2 31.731 208 127 5 61 255 49 254 1.93e-20 90.9
Msa0135740 AT4G04500.3 31.731 208 127 5 61 255 49 254 1.93e-20 90.9
Msa0135740 AT4G04500.1 31.731 208 127 5 61 255 49 254 2.03e-20 90.9
Msa0135740 AT4G23260.2 28.636 220 149 5 36 249 30 247 3.72e-20 90.1
Msa0135740 AT3G21920.1 30.204 245 141 11 32 258 40 272 4.60e-20 87.4
Msa0135740 AT4G23260.1 28.636 220 149 5 36 249 30 247 4.70e-20 89.7
Msa0135740 AT3G21920.2 30.204 245 141 11 32 258 62 294 4.76e-20 87.8
Msa0135740 AT1G63580.1 29.469 207 139 5 49 250 35 239 1.31e-19 86.3
Msa0135740 AT1G63550.1 28.972 214 145 4 40 250 37 246 1.43e-19 87.0
Msa0135740 AT1G63600.1 28.054 221 149 4 35 250 39 254 1.45e-19 87.0
Msa0135740 AT3G21930.1 29.796 245 142 11 32 258 40 272 1.58e-19 85.9
Msa0135740 AT3G22040.1 27.907 258 159 9 4 248 7 250 2.49e-19 85.1
Msa0135740 AT5G41300.1 26.531 245 171 4 6 247 7 245 1.22e-18 83.6
Msa0135740 AT1G63570.3 26.000 250 170 5 3 249 4 241 1.88e-18 83.2
Msa0135740 AT1G63570.1 26.000 250 170 5 3 249 4 241 1.88e-18 83.2
Msa0135740 AT1G63570.2 26.000 250 170 5 3 249 57 294 2.46e-18 83.6
Msa0135740 AT4G11460.2 31.609 174 104 5 87 248 1 171 3.83e-18 84.3
Msa0135740 AT3G29050.2 27.372 274 175 9 4 258 10 278 2.23e-17 80.1
Msa0135740 AT5G41280.1 27.935 247 160 6 12 249 7 244 2.45e-17 80.1
Msa0135740 AT4G11470.2 25.701 214 144 6 51 254 43 251 7.42e-17 80.5
Msa0135740 AT4G11470.1 25.701 214 144 6 51 254 38 246 7.68e-17 80.5
Msa0135740 AT4G11480.2 22.927 205 149 3 50 250 37 236 1.08e-16 79.7
Msa0135740 AT3G21940.1 29.091 275 162 12 2 255 12 274 1.15e-16 78.2
Msa0135740 AT4G11480.1 22.927 205 149 3 50 250 37 236 1.26e-16 79.7
Msa0135740 AT4G11480.3 22.927 205 149 3 50 250 37 236 1.36e-16 79.7
Msa0135740 AT4G11480.4 22.927 205 149 3 50 250 37 236 1.45e-16 79.7
Msa0135740 AT4G23190.3 31.361 169 104 5 89 248 1 166 1.70e-16 79.3
Msa0135740 AT4G23190.4 31.361 169 104 5 89 248 1 166 1.70e-16 79.3
Msa0135740 AT3G21940.2 32.778 180 102 6 92 255 3 179 2.06e-16 75.5
Msa0135740 AT3G22010.1 27.928 222 132 9 42 248 48 256 9.82e-16 75.1
Msa0135740 AT4G20680.1 28.342 187 123 5 71 248 76 260 4.23e-15 73.6
Msa0135740 AT3G21900.2 25.481 208 146 4 41 248 45 243 1.49e-14 72.0
Msa0135740 AT4G04570.2 27.179 195 132 4 68 254 59 251 1.99e-14 73.2
Msa0135740 AT4G04570.1 27.179 195 132 4 68 254 59 251 1.99e-14 73.2
Msa0135740 AT3G22050.1 26.941 219 134 6 43 248 46 251 2.07e-14 71.6
Msa0135740 AT4G23290.2 28.293 205 137 6 51 248 41 242 3.48e-14 72.4
Msa0135740 AT1G63590.1 25.405 185 131 3 71 250 64 246 7.21e-14 70.1
Msa0135740 AT3G22000.1 25.498 251 172 8 8 248 15 260 1.57e-13 69.3
Msa0135740 AT4G04510.1 28.283 198 119 6 71 254 60 248 1.71e-13 70.5
Msa0135740 AT4G04510.2 28.283 198 119 6 71 254 62 250 1.72e-13 70.5
Msa0135740 AT1G70690.1 27.805 205 124 8 49 248 48 233 2.46e-13 68.9
Msa0135740 AT3G21970.1 26.786 224 144 7 39 248 40 257 1.85e-12 66.2
Msa0135740 AT4G04540.4 23.111 225 158 6 43 254 32 254 2.34e-12 67.0
Msa0135740 AT4G04490.1 26.126 222 131 7 50 253 40 246 2.49e-12 67.0
Msa0135740 AT4G04540.1 23.111 225 158 6 43 254 32 254 3.17e-12 66.6
Msa0135740 AT4G04540.2 23.111 225 158 6 43 254 32 254 3.33e-12 66.6
Msa0135740 AT4G04540.3 23.111 225 158 6 43 254 32 254 3.72e-12 66.2
Msa0135740 AT2G01660.2 24.206 252 153 11 10 249 6 231 9.43e-12 63.9
Msa0135740 AT2G01660.3 24.206 252 153 11 10 249 6 231 1.31e-11 63.9
Msa0135740 AT2G01660.1 24.206 252 153 11 10 249 6 231 1.38e-11 63.9
Msa0135740 AT3G21900.1 24.038 208 141 4 41 248 45 235 3.75e-11 62.0

Find 48 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTAGGCCTAAACGAAAT+TGG 0.229067 1_3:+67469229 Msa0135740:CDS
TATTCAGACCGTTCTATATT+AGG 0.252445 1_3:+67469214 Msa0135740:CDS
AGTTACCTCTATCGTTATTT+TGG 0.283454 1_3:-67468928 None:intergenic
GGCAGATCAATTTAATATCT+TGG 0.285791 1_3:+67469291 Msa0135740:CDS
CGTTTACTTTGTCATTGTTT+TGG 0.299429 1_3:-67469051 None:intergenic
CTATCAACCTAAAGCAAAAC+TGG 0.325641 1_3:+67469576 Msa0135740:CDS
TTTCAAGATGTTGTAGCAAT+AGG 0.335675 1_3:+67469494 Msa0135740:CDS
TTGCTTTAGGTTGATAGAAT+TGG 0.343077 1_3:-67469570 None:intergenic
TTAAGAAGCAAGGCTATAAA+TGG 0.352413 1_3:+67469334 Msa0135740:CDS
TTGGTCGTTGTTGTCTGCTT+GGG 0.365814 1_3:-67468885 None:intergenic
AAGCAGGTCCAATTTCGTTT+AGG 0.394951 1_3:-67469237 None:intergenic
AGTAGTAATCAAATAATATA+TGG 0.412356 1_3:+67469400 Msa0135740:CDS
TTAGACCAAGCTGTAATTTG+AGG 0.412757 1_3:+67469533 Msa0135740:CDS
TTTGGTCGTTGTTGTCTGCT+TGG 0.426348 1_3:-67468886 None:intergenic
GATAATTATGAGAAAGAGAA+AGG 0.435798 1_3:-67468846 None:intergenic
CAGTGTACACCTGACTTGTC+TGG 0.440744 1_3:+67469430 Msa0135740:CDS
TGCTTGTGTGAGATTGAATC+TGG 0.442950 1_3:-67469134 None:intergenic
AATGACAAAGTAAACGTCAT+TGG 0.446473 1_3:+67469058 Msa0135740:CDS
AGGCATTTGCGACAATCATC+AGG 0.449621 1_3:-67469103 None:intergenic
TCACATCGTAAGTATGTTGT+AGG 0.450527 1_3:+67469361 Msa0135740:CDS
TGCGACCAAAATAACGATAG+AGG 0.460057 1_3:+67468923 Msa0135740:CDS
CTTGGGGTTTAGATGTGAAA+AGG 0.460200 1_3:-67468868 None:intergenic
TATATTATTTGATTACTACT+AGG 0.467816 1_3:-67469397 None:intergenic
GATAGAGGTAACTACATAAC+CGG 0.483653 1_3:+67468938 Msa0135740:CDS
TGAGAAAGAGAAAGGAGAGT+GGG 0.486566 1_3:-67468838 None:intergenic
GTTGAATGATTTAAGAAGCA+AGG 0.524724 1_3:+67469324 Msa0135740:CDS
TCAAACCTCAAATTACAGCT+TGG 0.526400 1_3:-67469538 None:intergenic
AGATGTTGTAGCAATAGGAT+TGG 0.527777 1_3:+67469499 Msa0135740:CDS
CACTGGGGACCAGACAAGTC+AGG 0.531473 1_3:-67469439 None:intergenic
GAGCAATTCTGAGGCTGTTG+TGG 0.536469 1_3:-67468970 None:intergenic
GATTGTCGCAAATGCCTAGT+TGG 0.540455 1_3:+67469109 Msa0135740:CDS
ATGAGAAAGAGAAAGGAGAG+TGG 0.541993 1_3:-67468839 None:intergenic
GCTGTTGTGGTAGGTATCAC+CGG 0.542628 1_3:-67468957 None:intergenic
AAGTTCCATGCAAAATAAGC+AGG 0.549615 1_3:-67469253 None:intergenic
CGTTTAGGCCTAATATAGAA+CGG 0.560797 1_3:-67469222 None:intergenic
GAACTTGAACAATGCAACAA+TGG 0.575248 1_3:+67469270 Msa0135740:CDS
GATTGAATCTGGAACCAACT+AGG 0.575788 1_3:-67469123 None:intergenic
TGGTCGTTGTTGTCTGCTTG+GGG 0.575882 1_3:-67468884 None:intergenic
AATTCTGAGGCTGTTGTGGT+AGG 0.584609 1_3:-67468966 None:intergenic
AAGCAAACAATCATCACACT+GGG 0.585932 1_3:-67469455 None:intergenic
GAAGCAAACAATCATCACAC+TGG 0.591047 1_3:-67469456 None:intergenic
TATGTTGTAGGAACAATGAC+GGG 0.594989 1_3:+67469373 Msa0135740:CDS
GGTTTCTACAACACCAACAA+TGG 0.608559 1_3:+67469028 Msa0135740:CDS
GTATGTTGTAGGAACAATGA+CGG 0.611472 1_3:+67469372 Msa0135740:CDS
AACGTCATTGGAATATGTAG+AGG 0.614721 1_3:+67469070 Msa0135740:CDS
AGAAGCAATTGGATGGTACG+AGG 0.670531 1_3:+67469171 Msa0135740:CDS
GGGAGATTAGAGCAATTCTG+AGG 0.693843 1_3:-67468979 None:intergenic
AGCAAACAATCATCACACTG+GGG 0.800516 1_3:-67469454 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTATTTGATTACTACT+AGG - chr1_3:67469400-67469419 None:intergenic 15.0%
!! AGTAGTAATCAAATAATATA+TGG + chr1_3:67469400-67469419 Msa0135740:CDS 15.0%
!! TATATTATTTGATTACTACT+AGG - chr1_3:67469400-67469419 None:intergenic 15.0%
!! AGTAGTAATCAAATAATATA+TGG + chr1_3:67469400-67469419 Msa0135740:CDS 15.0%
! GATAATTATGAGAAAGAGAA+AGG - chr1_3:67468849-67468868 None:intergenic 25.0%
!!! AATTTTTGAGTTGTAAGTGA+GGG - chr1_3:67469002-67469021 None:intergenic 25.0%
! TACAACTCAAAAATTGACTA+CGG + chr1_3:67469007-67469026 Msa0135740:CDS 25.0%
!!! CCAATTGCTTCTTTTTTATT+TGG - chr1_3:67469163-67469182 None:intergenic 25.0%
! CCAAATAAAAAAGAAGCAAT+TGG + chr1_3:67469160-67469179 Msa0135740:CDS 25.0%
! ATAAAAAAGAAGCAATTGGA+TGG + chr1_3:67469164-67469183 Msa0135740:CDS 25.0%
! GATAATTATGAGAAAGAGAA+AGG - chr1_3:67468849-67468868 None:intergenic 25.0%
!!! AATTTTTGAGTTGTAAGTGA+GGG - chr1_3:67469002-67469021 None:intergenic 25.0%
! TACAACTCAAAAATTGACTA+CGG + chr1_3:67469007-67469026 Msa0135740:CDS 25.0%
!!! CCAATTGCTTCTTTTTTATT+TGG - chr1_3:67469163-67469182 None:intergenic 25.0%
! CCAAATAAAAAAGAAGCAAT+TGG + chr1_3:67469160-67469179 Msa0135740:CDS 25.0%
! ATAAAAAAGAAGCAATTGGA+TGG + chr1_3:67469164-67469183 Msa0135740:CDS 25.0%
! AGTTACCTCTATCGTTATTT+TGG - chr1_3:67468931-67468950 None:intergenic 30.0%
!!! CAATTTTTGAGTTGTAAGTG+AGG - chr1_3:67469003-67469022 None:intergenic 30.0%
! CGTTTACTTTGTCATTGTTT+TGG - chr1_3:67469054-67469073 None:intergenic 30.0%
AATGACAAAGTAAACGTCAT+TGG + chr1_3:67469058-67469077 Msa0135740:CDS 30.0%
TATTCAGACCGTTCTATATT+AGG + chr1_3:67469214-67469233 Msa0135740:CDS 30.0%
ATATTAGGCCTAAACGAAAT+TGG + chr1_3:67469229-67469248 Msa0135740:CDS 30.0%
GGCAGATCAATTTAATATCT+TGG + chr1_3:67469291-67469310 Msa0135740:CDS 30.0%
GTTGAATGATTTAAGAAGCA+AGG + chr1_3:67469324-67469343 Msa0135740:CDS 30.0%
TTAAGAAGCAAGGCTATAAA+TGG + chr1_3:67469334-67469353 Msa0135740:CDS 30.0%
TTTCAAGATGTTGTAGCAAT+AGG + chr1_3:67469494-67469513 Msa0135740:CDS 30.0%
!! TTGCTTTAGGTTGATAGAAT+TGG - chr1_3:67469573-67469592 None:intergenic 30.0%
! AGTTACCTCTATCGTTATTT+TGG - chr1_3:67468931-67468950 None:intergenic 30.0%
!!! CAATTTTTGAGTTGTAAGTG+AGG - chr1_3:67469003-67469022 None:intergenic 30.0%
! CGTTTACTTTGTCATTGTTT+TGG - chr1_3:67469054-67469073 None:intergenic 30.0%
AATGACAAAGTAAACGTCAT+TGG + chr1_3:67469058-67469077 Msa0135740:CDS 30.0%
TATTCAGACCGTTCTATATT+AGG + chr1_3:67469214-67469233 Msa0135740:CDS 30.0%
ATATTAGGCCTAAACGAAAT+TGG + chr1_3:67469229-67469248 Msa0135740:CDS 30.0%
GGCAGATCAATTTAATATCT+TGG + chr1_3:67469291-67469310 Msa0135740:CDS 30.0%
GTTGAATGATTTAAGAAGCA+AGG + chr1_3:67469324-67469343 Msa0135740:CDS 30.0%
TTAAGAAGCAAGGCTATAAA+TGG + chr1_3:67469334-67469353 Msa0135740:CDS 30.0%
TTTCAAGATGTTGTAGCAAT+AGG + chr1_3:67469494-67469513 Msa0135740:CDS 30.0%
!! TTGCTTTAGGTTGATAGAAT+TGG - chr1_3:67469573-67469592 None:intergenic 30.0%
!! CGCAAAAGTAGTGGAATTTT+TGG - chr1_3:67468907-67468926 None:intergenic 35.0%
!!! TATTTTGGTCGCAAAAGTAG+TGG - chr1_3:67468916-67468935 None:intergenic 35.0%
GATAGAGGTAACTACATAAC+CGG + chr1_3:67468938-67468957 Msa0135740:CDS 35.0%
AACGTCATTGGAATATGTAG+AGG + chr1_3:67469070-67469089 Msa0135740:CDS 35.0%
CGTTTAGGCCTAATATAGAA+CGG - chr1_3:67469225-67469244 None:intergenic 35.0%
AAGTTCCATGCAAAATAAGC+AGG - chr1_3:67469256-67469275 None:intergenic 35.0%
GAACTTGAACAATGCAACAA+TGG + chr1_3:67469270-67469289 Msa0135740:CDS 35.0%
TCACATCGTAAGTATGTTGT+AGG + chr1_3:67469361-67469380 Msa0135740:CDS 35.0%
GTATGTTGTAGGAACAATGA+CGG + chr1_3:67469372-67469391 Msa0135740:CDS 35.0%
TATGTTGTAGGAACAATGAC+GGG + chr1_3:67469373-67469392 Msa0135740:CDS 35.0%
AAGCAAACAATCATCACACT+GGG - chr1_3:67469458-67469477 None:intergenic 35.0%
AGATGTTGTAGCAATAGGAT+TGG + chr1_3:67469499-67469518 Msa0135740:CDS 35.0%
TTAGACCAAGCTGTAATTTG+AGG + chr1_3:67469533-67469552 Msa0135740:CDS 35.0%
TCAAACCTCAAATTACAGCT+TGG - chr1_3:67469541-67469560 None:intergenic 35.0%
CTATCAACCTAAAGCAAAAC+TGG + chr1_3:67469576-67469595 Msa0135740:CDS 35.0%
!! CGCAAAAGTAGTGGAATTTT+TGG - chr1_3:67468907-67468926 None:intergenic 35.0%
!!! TATTTTGGTCGCAAAAGTAG+TGG - chr1_3:67468916-67468935 None:intergenic 35.0%
GATAGAGGTAACTACATAAC+CGG + chr1_3:67468938-67468957 Msa0135740:CDS 35.0%
AACGTCATTGGAATATGTAG+AGG + chr1_3:67469070-67469089 Msa0135740:CDS 35.0%
CGTTTAGGCCTAATATAGAA+CGG - chr1_3:67469225-67469244 None:intergenic 35.0%
AAGTTCCATGCAAAATAAGC+AGG - chr1_3:67469256-67469275 None:intergenic 35.0%
GAACTTGAACAATGCAACAA+TGG + chr1_3:67469270-67469289 Msa0135740:CDS 35.0%
TCACATCGTAAGTATGTTGT+AGG + chr1_3:67469361-67469380 Msa0135740:CDS 35.0%
GTATGTTGTAGGAACAATGA+CGG + chr1_3:67469372-67469391 Msa0135740:CDS 35.0%
TATGTTGTAGGAACAATGAC+GGG + chr1_3:67469373-67469392 Msa0135740:CDS 35.0%
AAGCAAACAATCATCACACT+GGG - chr1_3:67469458-67469477 None:intergenic 35.0%
AGATGTTGTAGCAATAGGAT+TGG + chr1_3:67469499-67469518 Msa0135740:CDS 35.0%
TTAGACCAAGCTGTAATTTG+AGG + chr1_3:67469533-67469552 Msa0135740:CDS 35.0%
TCAAACCTCAAATTACAGCT+TGG - chr1_3:67469541-67469560 None:intergenic 35.0%
CTATCAACCTAAAGCAAAAC+TGG + chr1_3:67469576-67469595 Msa0135740:CDS 35.0%
TGAGAAAGAGAAAGGAGAGT+GGG - chr1_3:67468841-67468860 None:intergenic 40.0%
ATGAGAAAGAGAAAGGAGAG+TGG - chr1_3:67468842-67468861 None:intergenic 40.0%
CTTGGGGTTTAGATGTGAAA+AGG - chr1_3:67468871-67468890 None:intergenic 40.0%
TGCGACCAAAATAACGATAG+AGG + chr1_3:67468923-67468942 Msa0135740:CDS 40.0%
GGTTTCTACAACACCAACAA+TGG + chr1_3:67469028-67469047 Msa0135740:CDS 40.0%
!!! GTCATTGTTTTGGCCATTGT+TGG - chr1_3:67469044-67469063 None:intergenic 40.0%
! GATTGAATCTGGAACCAACT+AGG - chr1_3:67469126-67469145 None:intergenic 40.0%
TGCTTGTGTGAGATTGAATC+TGG - chr1_3:67469137-67469156 None:intergenic 40.0%
AAGCAGGTCCAATTTCGTTT+AGG - chr1_3:67469240-67469259 None:intergenic 40.0%
!!! TTGGACCTGCTTATTTTGCA+TGG + chr1_3:67469248-67469267 Msa0135740:CDS 40.0%
AGCAAACAATCATCACACTG+GGG - chr1_3:67469457-67469476 None:intergenic 40.0%
GAAGCAAACAATCATCACAC+TGG - chr1_3:67469459-67469478 None:intergenic 40.0%
! TGTGAAGCCAGTTTTGCTTT+AGG - chr1_3:67469586-67469605 None:intergenic 40.0%
TGAGAAAGAGAAAGGAGAGT+GGG - chr1_3:67468841-67468860 None:intergenic 40.0%
ATGAGAAAGAGAAAGGAGAG+TGG - chr1_3:67468842-67468861 None:intergenic 40.0%
CTTGGGGTTTAGATGTGAAA+AGG - chr1_3:67468871-67468890 None:intergenic 40.0%
TGCGACCAAAATAACGATAG+AGG + chr1_3:67468923-67468942 Msa0135740:CDS 40.0%
GGTTTCTACAACACCAACAA+TGG + chr1_3:67469028-67469047 Msa0135740:CDS 40.0%
!!! GTCATTGTTTTGGCCATTGT+TGG - chr1_3:67469044-67469063 None:intergenic 40.0%
! GATTGAATCTGGAACCAACT+AGG - chr1_3:67469126-67469145 None:intergenic 40.0%
TGCTTGTGTGAGATTGAATC+TGG - chr1_3:67469137-67469156 None:intergenic 40.0%
AAGCAGGTCCAATTTCGTTT+AGG - chr1_3:67469240-67469259 None:intergenic 40.0%
!!! TTGGACCTGCTTATTTTGCA+TGG + chr1_3:67469248-67469267 Msa0135740:CDS 40.0%
AGCAAACAATCATCACACTG+GGG - chr1_3:67469457-67469476 None:intergenic 40.0%
GAAGCAAACAATCATCACAC+TGG - chr1_3:67469459-67469478 None:intergenic 40.0%
! TGTGAAGCCAGTTTTGCTTT+AGG - chr1_3:67469586-67469605 None:intergenic 40.0%
TTGGTCGTTGTTGTCTGCTT+GGG - chr1_3:67468888-67468907 None:intergenic 45.0%
TTTGGTCGTTGTTGTCTGCT+TGG - chr1_3:67468889-67468908 None:intergenic 45.0%
!! AATTCTGAGGCTGTTGTGGT+AGG - chr1_3:67468969-67468988 None:intergenic 45.0%
GGGAGATTAGAGCAATTCTG+AGG - chr1_3:67468982-67469001 None:intergenic 45.0%
AGGCATTTGCGACAATCATC+AGG - chr1_3:67469106-67469125 None:intergenic 45.0%
GATTGTCGCAAATGCCTAGT+TGG + chr1_3:67469109-67469128 Msa0135740:CDS 45.0%
AGAAGCAATTGGATGGTACG+AGG + chr1_3:67469171-67469190 Msa0135740:CDS 45.0%
TTGGTCGTTGTTGTCTGCTT+GGG - chr1_3:67468888-67468907 None:intergenic 45.0%
TTTGGTCGTTGTTGTCTGCT+TGG - chr1_3:67468889-67468908 None:intergenic 45.0%
!! AATTCTGAGGCTGTTGTGGT+AGG - chr1_3:67468969-67468988 None:intergenic 45.0%
GGGAGATTAGAGCAATTCTG+AGG - chr1_3:67468982-67469001 None:intergenic 45.0%
AGGCATTTGCGACAATCATC+AGG - chr1_3:67469106-67469125 None:intergenic 45.0%
GATTGTCGCAAATGCCTAGT+TGG + chr1_3:67469109-67469128 Msa0135740:CDS 45.0%
AGAAGCAATTGGATGGTACG+AGG + chr1_3:67469171-67469190 Msa0135740:CDS 45.0%
TGGTCGTTGTTGTCTGCTTG+GGG - chr1_3:67468887-67468906 None:intergenic 50.0%
GCTGTTGTGGTAGGTATCAC+CGG - chr1_3:67468960-67468979 None:intergenic 50.0%
!! GAGCAATTCTGAGGCTGTTG+TGG - chr1_3:67468973-67468992 None:intergenic 50.0%
! CAGTGTACACCTGACTTGTC+TGG + chr1_3:67469430-67469449 Msa0135740:CDS 50.0%
TGGTCGTTGTTGTCTGCTTG+GGG - chr1_3:67468887-67468906 None:intergenic 50.0%
GCTGTTGTGGTAGGTATCAC+CGG - chr1_3:67468960-67468979 None:intergenic 50.0%
!! GAGCAATTCTGAGGCTGTTG+TGG - chr1_3:67468973-67468992 None:intergenic 50.0%
! CAGTGTACACCTGACTTGTC+TGG + chr1_3:67469430-67469449 Msa0135740:CDS 50.0%
CACTGGGGACCAGACAAGTC+AGG - chr1_3:67469442-67469461 None:intergenic 60.0%
CACTGGGGACCAGACAAGTC+AGG - chr1_3:67469442-67469461 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 67468818 67469615 67468818 ID=Msa0135740;Name=Msa0135740
chr1_3 mRNA 67468818 67469615 67468818 ID=Msa0135740-mRNA-1;Parent=Msa0135740;Name=Msa0135740-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|-1|0|1|-1|1|1|0|265
chr1_3 exon 67468818 67469615 67468818 ID=Msa0135740-mRNA-1:exon:23133;Parent=Msa0135740-mRNA-1
chr1_3 CDS 67468818 67469615 67468818 ID=Msa0135740-mRNA-1:cds;Parent=Msa0135740-mRNA-1
Gene Sequence

>Msa0135740

ATGTCTATTCTCTCTTTAAGCCCACTCTCCTTTCTCTTTCTCATAATTATCCTTTTCACATCTAAACCCCAAGCAGACAACAACGACCAAAAATTCCACTACTTTTGCGACCAAAATAACGATAGAGGTAACTACATAACCGGTGATACCTACCACAACAGCCTCAGAATTGCTCTAATCTCCCTCACTTACAACTCAAAAATTGACTACGGTTTCTACAACACCAACAATGGCCAAAACAATGACAAAGTAAACGTCATTGGAATATGTAGAGGAGACATTAATCCTGATGATTGTCGCAAATGCCTAGTTGGTTCCAGATTCAATCTCACACAAGCATGTCCAAATAAAAAAGAAGCAATTGGATGGTACGAGGATGAGAAATGCATGTTACGTTATTCAGACCGTTCTATATTAGGCCTAAACGAAATTGGACCTGCTTATTTTGCATGGAACTTGAACAATGCAACAATGGCAGATCAATTTAATATCTTGGTAAAACAACTGTTGAATGATTTAAGAAGCAAGGCTATAAATGGTGATTCACATCGTAAGTATGTTGTAGGAACAATGACGGGTCCTAGTAGTAATCAAATAATATATGGTCTTGTTCAGTGTACACCTGACTTGTCTGGTCCCCAGTGTGATGATTGTTTGCTTCAATCTATTGCTGAAGTTTCAAGATGTTGTAGCAATAGGATTGGTGCTAGAATTGTTAGACCAAGCTGTAATTTGAGGTTTGAAACTTCATACCAATTCTATCAACCTAAAGCAAAACTGGCTTCACATCTCCCATGA
Protein sequence

>Msa0135740

MSILSLSPLSFLFLIIILFTSKPQADNNDQKFHYFCDQNNDRGNYITGDTYHNSLRIALISLTYNSKIDYGFYNTNNGQNNDKVNVIGICRGDINPDDCRKCLVGSRFNLTQACPNKKEAIGWYEDEKCMLRYSDRSILGLNEIGPAYFAWNLNNATMADQFNILVKQLLNDLRSKAINGDSHRKYVVGTMTGPSSNQIIYGLVQCTPDLSGPQCDDCLLQSIAEVSRCCSNRIGARIVRPSCNLRFETSYQFYQPKAKLASHLP*