AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0253180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0253180 MtrunA17_Chr2g0312571 92.135 89 6 1 71 159 225 312 4.06e-51 169
Msa0253180 MtrunA17_Chr2g0312571 88.710 62 6 1 1 61 1 62 1.53e-31 117
Msa0253180 MtrunA17_Chr2g0312561 87.640 89 10 1 71 159 220 307 3.62e-48 160
Msa0253180 MtrunA17_Chr2g0312561 80.328 61 10 1 1 61 1 59 2.83e-25 99.8
Msa0253180 MtrunA17_Chr8g0358911 75.556 90 21 1 70 159 222 310 2.18e-41 142
Msa0253180 MtrunA17_Chr8g0358911 64.516 62 19 2 1 60 1 61 4.45e-18 80.1
Msa0253180 MtrunA17_Chr8g0360591 62.222 90 33 1 70 159 220 308 4.21e-33 120
Msa0253180 MtrunA17_Chr8g0360591 63.333 60 21 1 1 60 1 59 7.44e-15 70.9
Msa0253180 MtrunA17_Chr8g0358901 43.056 144 71 3 17 159 190 323 3.12e-23 93.6
Msa0253180 MtrunA17_Chr1g0181341 45.652 92 48 2 68 159 222 311 2.56e-21 89.0
Msa0253180 MtrunA17_Chr8g0358821 46.667 90 46 2 70 159 863 950 9.93e-19 82.4
Msa0253180 MtrunA17_Chr3g0109411 45.055 91 48 2 70 159 500 589 2.23e-18 81.3
Msa0253180 MtrunA17_Chr8g0358891 41.818 110 52 3 44 150 54 154 2.88e-18 76.6
Msa0253180 MtrunA17_Chr8g0358781 45.556 90 47 2 70 159 518 605 4.74e-18 80.5
Msa0253180 MtrunA17_Chr2g0315151 45.455 88 48 0 70 157 842 929 3.78e-17 77.8
Msa0253180 MtrunA17_Chr5g0443531 45.556 90 48 1 70 159 854 942 5.16e-17 77.4
Msa0253180 MtrunA17_Chr4g0058891 40.659 91 51 2 69 159 461 548 5.77e-17 77.0
Msa0253180 MtrunA17_Chr3g0109431 39.560 91 53 2 70 159 503 592 1.64e-16 75.9
Msa0253180 MtrunA17_Chr8g0359691 43.956 91 48 3 70 159 840 928 3.96e-16 74.7
Msa0253180 MtrunA17_Chr2g0281971 32.824 131 69 4 44 159 186 312 5.29e-16 73.9
Msa0253180 MtrunA17_Chr2g0314991 42.045 88 51 0 70 157 1353 1440 6.47e-16 74.3
Msa0253180 MtrunA17_Chr2g0314991 38.636 88 54 0 70 157 387 474 5.20e-12 63.2
Msa0253180 MtrunA17_Chr4g0071371 41.758 91 51 2 70 159 311 400 1.79e-15 72.8
Msa0253180 MtrunA17_Chr2g0299041 46.914 81 42 1 70 149 289 369 2.52e-15 72.4
Msa0253180 MtrunA17_Chr2g0315081 42.222 90 51 1 70 159 830 918 6.14e-15 71.2
Msa0253180 MtrunA17_Chr2g0314961 42.222 90 51 1 70 159 312 400 7.73e-15 70.9
Msa0253180 MtrunA17_Chr3g0109461 39.130 92 53 3 70 159 515 605 2.45e-14 69.7
Msa0253180 MtrunA17_Chr8g0357691 39.326 89 53 1 71 159 656 743 1.08e-13 67.8
Msa0253180 MtrunA17_Chr2g0314861 41.758 91 50 3 70 159 96 184 1.32e-13 66.6
Msa0253180 MtrunA17_Chr7g0226891 42.222 90 49 2 70 159 225 311 1.70e-13 67.0
Msa0253180 MtrunA17_Chr6g0484231 38.636 88 52 2 70 157 225 310 2.05e-13 66.6
Msa0253180 MtrunA17_Chr7g0274651 41.026 78 46 0 71 148 546 623 2.20e-13 67.0
Msa0253180 MtrunA17_Chr8g0359651 37.879 132 75 5 29 159 806 931 3.91e-13 66.2
Msa0253180 MtrunA17_Chr6g0474991 33.333 90 58 2 71 159 41 129 4.24e-13 63.9
Msa0253180 MtrunA17_Chr8g0359641 37.879 132 75 5 29 159 806 931 4.26e-13 66.2
Msa0253180 MtrunA17_Chr3g0081101 36.364 88 55 1 70 157 15 101 4.86e-13 63.2
Msa0253180 MtrunA17_Chr1g0206361 31.897 116 77 2 45 159 494 608 5.74e-13 65.9
Msa0253180 MtrunA17_Chr6g0475201 36.264 91 56 2 71 160 752 841 6.34e-13 65.5
Msa0253180 MtrunA17_Chr7g0242201 36.364 88 54 2 72 159 222 307 7.80e-13 65.1
Msa0253180 MtrunA17_Chr6g0475131 35.165 91 57 2 71 160 187 276 9.01e-13 64.7
Msa0253180 MtrunA17_Chr2g0314881 35.878 131 75 4 36 159 365 493 9.18e-13 65.1
Msa0253180 MtrunA17_Chr6g0474901 29.577 142 90 4 20 160 701 833 1.29e-12 64.7
Msa0253180 MtrunA17_Chr5g0394001 34.444 90 58 1 70 159 507 595 1.33e-12 64.7
Msa0253180 MtrunA17_Chr2g0314901 39.773 88 53 0 70 157 845 932 1.38e-12 64.7
Msa0253180 MtrunA17_Chr8g0357661 35.556 135 75 7 29 159 2 128 1.46e-12 62.4
Msa0253180 MtrunA17_Chr2g0314911 42.500 80 46 0 70 149 838 917 1.48e-12 64.7
Msa0253180 MtrunA17_Chr4g0048801 34.831 89 57 1 71 159 696 783 1.55e-12 64.3
Msa0253180 MtrunA17_Chr3g0084491 36.264 91 56 2 70 159 683 772 1.75e-12 64.3
Msa0253180 MtrunA17_Chr3g0084451 36.667 90 55 2 71 159 684 772 2.36e-12 63.9
Msa0253180 MtrunA17_Chr3g0084441 36.667 90 55 2 71 159 684 772 2.62e-12 63.9
Msa0253180 MtrunA17_Chr8g0357651 35.955 89 56 1 71 159 648 735 4.30e-12 63.2
Msa0253180 MtrunA17_Chr3g0081031 34.351 131 82 2 29 159 491 617 4.39e-12 63.2
Msa0253180 MtrunA17_Chr2g0297921 40.230 87 51 1 73 159 322 407 4.61e-12 62.8
Msa0253180 MtrunA17_Chr7g0274661 38.462 78 48 0 71 148 550 627 4.70e-12 63.2
Msa0253180 MtrunA17_Chr2g0297951 40.230 87 51 1 73 159 312 397 5.01e-12 62.8
Msa0253180 MtrunA17_Chr2g0327471 35.556 90 56 2 71 159 270 358 5.27e-12 62.8
Msa0253180 MtrunA17_Chr6g0474861 31.667 120 75 5 42 160 440 553 7.04e-12 62.4
Msa0253180 MtrunA17_Chr4g0023111 37.931 87 53 1 71 157 892 977 7.96e-12 62.4
Msa0253180 MtrunA17_Chr8g0357761 37.634 93 51 4 70 159 657 745 8.18e-12 62.4
Msa0253180 MtrunA17_Chr2g0280681 36.667 90 55 2 71 159 679 767 8.41e-12 62.4
Msa0253180 MtrunA17_Chr3g0081071 35.955 89 56 1 71 159 107 194 9.80e-12 61.2
Msa0253180 MtrunA17_Chr4g0048731 32.222 90 60 1 71 160 224 312 1.03e-11 61.6
Msa0253180 MtrunA17_Chr1g0189791 34.444 90 57 2 71 159 250 338 1.25e-11 61.6
Msa0253180 MtrunA17_Chr4g0074651 36.667 90 55 2 71 159 302 390 1.54e-11 61.2
Msa0253180 MtrunA17_Chr3g0081001 37.079 89 55 1 71 159 521 608 1.74e-11 61.2
Msa0253180 MtrunA17_Chr4g0048741 31.868 91 61 1 70 160 709 798 1.77e-11 61.2
Msa0253180 MtrunA17_Chr8g0376131 32.584 89 59 1 71 159 229 316 1.85e-11 61.2
Msa0253180 MtrunA17_Chr2g0314821 39.560 91 52 3 70 159 840 928 1.92e-11 61.2
Msa0253180 MtrunA17_Chr3g0078521 34.066 91 59 1 71 161 697 786 1.99e-11 61.2
Msa0253180 MtrunA17_Chr6g0474871 30.833 120 76 5 42 160 719 832 2.02e-11 61.2
Msa0253180 MtrunA17_Chr8g0361141 37.079 89 54 2 71 159 694 780 2.10e-11 61.2
Msa0253180 MtrunA17_Chr2g0314841 39.560 91 52 3 70 159 837 925 2.17e-11 61.2
Msa0253180 MtrunA17_Chr1g0206321 31.111 90 60 2 71 159 9 97 2.19e-11 58.9
Msa0253180 MtrunA17_Chr3g0102451 32.787 122 64 5 44 159 480 589 2.33e-11 60.8
Msa0253180 MtrunA17_Chr2g0280781 36.667 90 56 1 70 159 687 775 2.38e-11 60.8
Msa0253180 MtrunA17_Chr4g0047561 38.202 89 53 2 71 159 224 310 2.45e-11 60.8
Msa0253180 MtrunA17_Chr2g0280761 34.444 90 58 1 70 159 8 96 2.72e-11 58.5
Msa0253180 MtrunA17_Chr3g0078541 34.066 91 59 1 71 161 708 797 2.81e-11 60.8
Msa0253180 MtrunA17_Chr6g0475091 32.710 107 66 4 54 159 746 847 2.92e-11 60.8
Msa0253180 MtrunA17_Chr6g0474811 31.868 91 60 2 71 160 751 840 3.63e-11 60.5
Msa0253180 MtrunA17_Chr4g0048791 33.333 90 59 1 71 160 710 798 3.71e-11 60.5
Msa0253180 MtrunA17_Chr8g0357831 34.831 89 57 1 71 159 370 457 3.73e-11 60.5
Msa0253180 MtrunA17_Chr4g0048581 38.202 89 53 2 71 159 709 795 3.74e-11 60.5
Msa0253180 MtrunA17_Chr8g0361161 37.079 89 54 2 71 159 222 308 4.01e-11 60.1
Msa0253180 MtrunA17_Chr8g0357631 28.777 139 87 3 21 159 607 733 4.05e-11 60.5
Msa0253180 MtrunA17_Chr4g0048711 31.111 90 61 1 71 160 107 195 4.66e-11 60.1
Msa0253180 MtrunA17_Chr7g0239381 30.337 89 61 1 71 159 519 606 4.81e-11 60.1
Msa0253180 MtrunA17_Chr8g0347571 31.111 90 61 1 71 160 748 836 4.89e-11 60.1
Msa0253180 MtrunA17_Chr3g0082941 34.831 89 57 1 71 159 351 438 4.95e-11 60.1
Msa0253180 MtrunA17_Chr8g0380441 32.222 90 59 2 71 159 255 343 5.01e-11 59.7
Msa0253180 MtrunA17_Chr2g0280751 35.955 89 55 2 71 159 704 790 5.54e-11 60.1
Msa0253180 MtrunA17_Chr1g0206221 32.258 93 56 4 70 159 2 90 5.78e-11 57.4
Msa0253180 MtrunA17_Chr3g0084681 34.066 91 58 2 70 159 641 730 5.92e-11 59.7
Msa0253180 MtrunA17_Chr8g0357711 36.957 92 51 4 71 159 655 742 6.12e-11 59.7
Msa0253180 MtrunA17_Chr1g0206351 34.444 90 58 1 70 159 530 618 6.17e-11 59.7
Msa0253180 MtrunA17_Chr3g0078761 28.276 145 88 4 22 161 478 611 6.86e-11 59.7
Msa0253180 MtrunA17_Chr2g0315011 39.241 79 48 0 70 148 16 94 7.10e-11 58.2
Msa0253180 MtrunA17_Chr3g0078551 32.222 90 60 1 71 160 702 790 7.46e-11 59.7
Msa0253180 MtrunA17_Chr2g0315061 37.778 90 55 1 70 159 213 301 9.21e-11 58.9
Msa0253180 MtrunA17_Chr8g0340091 37.931 87 53 1 71 157 710 795 9.22e-11 59.3
Msa0253180 MtrunA17_Chr8g0340111 33.884 121 72 6 38 157 655 768 9.72e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0253180 AT1G16670.1 72.222 90 24 1 70 159 219 307 1.02e-39 138
Msa0253180 AT1G16670.1 73.684 38 10 0 23 60 19 56 4.72e-12 63.2
Msa0253180 AT1G56130.3 43.333 90 49 2 70 159 669 756 6.12e-17 77.4
Msa0253180 AT1G56130.1 43.333 90 49 2 70 159 720 807 6.67e-17 77.4
Msa0253180 AT1G56130.2 43.333 90 49 2 70 159 868 955 7.92e-17 77.4
Msa0253180 AT1G56140.1 44.444 90 48 2 70 159 867 954 9.79e-17 77.0
Msa0253180 AT5G40380.1 46.316 95 43 4 70 159 490 581 1.17e-16 76.6
Msa0253180 AT1G07650.4 44.444 90 49 1 70 159 642 730 1.71e-16 76.3
Msa0253180 AT3G14840.2 41.111 90 52 1 70 159 848 936 1.92e-16 76.3
Msa0253180 AT1G07650.3 44.444 90 49 1 70 159 829 917 2.11e-16 75.9
Msa0253180 AT1G07650.1 44.444 90 49 1 70 159 855 943 2.15e-16 75.9
Msa0253180 AT1G07650.2 44.444 90 49 1 70 159 861 949 2.35e-16 75.9
Msa0253180 AT4G23260.2 39.560 91 53 2 70 159 504 593 2.72e-16 75.5
Msa0253180 AT4G23260.1 39.560 91 53 2 70 159 515 604 2.83e-16 75.5
Msa0253180 AT1G53430.2 46.591 88 47 0 70 157 804 891 3.90e-16 75.1
Msa0253180 AT1G53440.1 46.591 88 47 0 70 157 843 930 3.96e-16 75.1
Msa0253180 AT1G53430.1 46.591 88 47 0 70 157 845 932 4.07e-16 75.1
Msa0253180 AT1G56120.3 42.222 90 50 2 70 159 718 805 2.32e-15 73.2
Msa0253180 AT1G56120.1 42.222 90 50 2 70 159 884 971 2.42e-15 73.2
Msa0253180 AT1G56120.2 42.222 90 50 2 70 159 866 953 2.44e-15 73.2
Msa0253180 AT1G29720.1 38.202 89 54 1 71 159 848 935 4.25e-15 72.4
Msa0253180 AT3G09010.1 32.824 131 69 4 44 159 183 309 2.54e-14 69.7
Msa0253180 AT1G70530.1 40.244 82 47 1 70 151 498 577 3.24e-14 69.7
Msa0253180 AT1G56145.3 38.202 89 53 2 71 159 759 845 8.11e-14 68.6
Msa0253180 AT1G56145.1 38.202 89 53 2 71 159 862 948 8.87e-14 68.6
Msa0253180 AT4G23160.1 39.326 89 53 1 71 159 536 623 9.07e-14 68.6
Msa0253180 AT1G56145.2 38.202 89 53 2 71 159 889 975 1.12e-13 68.2
Msa0253180 AT3G55550.1 34.409 93 60 1 67 159 519 610 1.48e-13 67.8
Msa0253180 AT4G23140.2 38.889 90 54 1 70 159 533 621 1.54e-13 67.8
Msa0253180 AT4G23250.3 35.165 91 57 2 70 159 530 619 1.55e-13 67.8
Msa0253180 AT4G23140.1 38.889 90 54 1 70 159 527 615 1.56e-13 67.8
Msa0253180 AT4G23250.2 35.165 91 57 2 70 159 530 619 1.62e-13 67.8
Msa0253180 AT4G23250.1 35.165 91 57 2 70 159 535 624 1.69e-13 67.8
Msa0253180 AT1G53420.1 36.792 106 62 3 54 159 788 888 2.24e-13 67.4
Msa0253180 AT1G11340.1 39.773 88 48 3 71 155 692 777 7.96e-13 65.9
Msa0253180 AT1G11410.4 39.773 88 49 3 71 157 707 791 1.22e-12 65.1
Msa0253180 AT4G23210.3 34.234 111 62 4 48 157 518 618 1.22e-12 65.1
Msa0253180 AT1G11410.1 39.773 88 49 3 71 157 700 784 1.26e-12 65.1
Msa0253180 AT1G11410.3 39.773 88 49 3 71 157 676 760 1.27e-12 65.1
Msa0253180 AT1G70520.1 32.090 134 81 4 30 157 464 593 1.32e-12 65.1
Msa0253180 AT1G11410.2 39.773 88 49 3 71 157 585 669 1.40e-12 65.1
Msa0253180 AT4G03230.4 37.079 89 55 1 71 159 867 954 1.42e-12 65.1
Msa0253180 AT4G03230.1 37.079 89 55 1 71 159 867 954 1.42e-12 65.1
Msa0253180 AT4G11460.1 36.264 91 56 2 70 159 525 614 1.49e-12 64.7
Msa0253180 AT4G03230.5 37.079 89 55 1 71 159 795 882 1.52e-12 65.1
Msa0253180 AT4G11460.2 36.264 91 56 2 70 159 447 536 1.56e-12 64.7
Msa0253180 AT4G03230.7 37.079 89 55 1 71 159 710 797 1.58e-12 64.7
Msa0253180 AT4G03230.8 37.079 89 55 1 71 159 709 796 1.59e-12 64.7
Msa0253180 AT4G11460.3 36.264 91 56 2 70 159 522 611 1.61e-12 64.7
Msa0253180 AT4G03230.2 37.079 89 55 1 71 159 767 854 1.64e-12 64.7
Msa0253180 AT4G03230.6 37.079 89 55 1 71 159 767 854 1.64e-12 64.7
Msa0253180 AT4G03230.3 37.079 89 55 1 71 159 702 789 1.64e-12 64.7
Msa0253180 AT4G11530.1 34.586 133 80 5 29 159 490 617 2.66e-12 64.3
Msa0253180 AT4G23320.3 35.556 90 56 2 71 159 381 469 2.98e-12 63.9
Msa0253180 AT4G23320.2 35.556 90 56 2 71 159 360 448 3.09e-12 63.9
Msa0253180 AT4G23320.1 35.556 90 56 2 71 159 360 448 3.09e-12 63.9
Msa0253180 AT4G28670.1 29.204 113 79 1 47 159 487 598 4.00e-12 63.5
Msa0253180 AT4G23290.2 34.444 90 57 2 71 159 540 628 4.57e-12 63.5
Msa0253180 AT1G29740.1 36.090 133 77 5 29 159 814 940 4.94e-12 63.5
Msa0253180 AT1G29740.2 36.090 133 77 5 29 159 814 940 5.24e-12 63.5
Msa0253180 AT4G23290.1 34.444 90 57 2 71 159 497 585 5.52e-12 63.2
Msa0253180 AT1G70740.1 36.082 97 58 1 71 163 238 334 8.11e-12 62.8
Msa0253180 AT5G60280.1 36.842 114 66 5 48 160 491 599 9.12e-12 62.8
Msa0253180 AT1G29750.1 36.111 108 62 2 52 159 830 930 9.59e-12 62.8
Msa0253180 AT1G29750.2 36.111 108 62 2 52 159 845 945 9.79e-12 62.8
Msa0253180 AT4G23150.1 35.556 90 57 1 70 159 512 600 9.94e-12 62.4
Msa0253180 AT5G60320.1 33.333 75 50 0 74 148 525 599 2.15e-11 61.6
Msa0253180 AT4G29450.1 32.184 87 58 1 73 159 756 841 3.21e-11 61.2
Msa0253180 AT4G11900.9 28.058 139 88 3 21 159 606 732 8.66e-11 59.7
Msa0253180 AT1G29730.1 39.286 84 48 2 70 151 815 897 8.82e-11 59.7
Msa0253180 AT1G29730.2 39.286 84 48 2 70 151 847 929 8.85e-11 59.7
Msa0253180 AT4G11900.1 28.058 139 88 3 21 159 675 801 9.55e-11 59.7

Find 71 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATCTCGAAGTGCATTTC+TGG 0.148202 2_2:-44795661 Msa0253180:three_prime_UTR
GGTCTATTTCAGAGGTTATT+TGG 0.185328 2_2:-44796735 Msa0253180:intron
GGGTACATACGTAGTGGTTT+TGG 0.258293 2_2:-44795560 Msa0253180:three_prime_UTR
GTCCATAAAATCAGAAATTA+AGG 0.279082 2_2:+44796019 None:intergenic
AAAATCGGAGAGGGTGGTTT+TGG 0.281450 2_2:-44797596 Msa0253180:CDS
GTACTTTGAACAGATATTTC+AGG 0.300098 2_2:-44797689 Msa0253180:intron
AAGGCAGACATTTACAGTTT+TGG 0.329602 2_2:-44796679 Msa0253180:CDS
CTTGTTTATGTCTAACTTTG+AGG 0.338625 2_2:+44795997 None:intergenic
AATGTACTGTTGTGTAAATA+TGG 0.354434 2_2:-44795508 Msa0253180:three_prime_UTR
AGAATGAATTGTTCTTCTAT+TGG 0.357558 2_2:+44796604 None:intergenic
TAGTAGATACATTGCTAAAC+GGG 0.366772 2_2:-44796189 Msa0253180:CDS
TTTGGCACCGAGTATGCAAT+AGG 0.385328 2_2:-44796717 Msa0253180:CDS
GCATGTAAGTTTCTGAAGAT+TGG 0.400687 2_2:-44796148 Msa0253180:CDS
CTAGTAGATACATTGCTAAA+CGG 0.421479 2_2:-44796190 Msa0253180:CDS
ATCCTCGGCCTCAGAGCATC+AGG 0.425394 2_2:-44795934 Msa0253180:three_prime_UTR
TTGGCACCGAGTATGCAATA+GGG 0.438175 2_2:-44796716 Msa0253180:CDS
TACGAGCGAAAGGAATTAGT+TGG 0.440671 2_2:-44796214 Msa0253180:CDS
AGCATTCCTTAGTTCTTTGT+AGG 0.442371 2_2:+44797643 None:intergenic
TTTAGTCCGGCCAATAAAAT+CGG 0.448805 2_2:-44797611 Msa0253180:CDS
GTCATCGACTTTCATTTCTC+CGG 0.452822 2_2:+44796061 None:intergenic
AGACATGGACGGTCGGCTCT+TGG 0.457396 2_2:+44796103 None:intergenic
ACTGAACGAGAAAGGGAAAC+AGG 0.469951 2_2:+44798208 None:intergenic
CGGGGAATTTGATGCCGAGC+AGG 0.470321 2_2:-44796170 Msa0253180:CDS
CAACCAGGTGACATTGGAAG+TGG 0.471384 2_2:-44798295 Msa0253180:five_prime_UTR
GGTTTCTTACTGAACGAGAA+AGG 0.479949 2_2:+44798200 None:intergenic
ACTGTGGTCAAGATGCTAAC+CGG 0.483980 2_2:-44796080 Msa0253180:exon
TCTATACCTACAAAGAACTA+AGG 0.485741 2_2:-44797649 Msa0253180:CDS
GAGATTCCTGAGTGCAAAGA+AGG 0.491129 2_2:+44796125 None:intergenic
CGAAGATGAAGTCTTCATAT+CGG 0.500901 2_2:+44795965 None:intergenic
AGTGGCAGCTGATGATATAA+TGG 0.510850 2_2:+44795905 None:intergenic
GACAAAGACAAATTATGAGA+AGG 0.511560 2_2:+44795415 None:intergenic
GATTGGCCTTCTTTGCACTC+AGG 0.514824 2_2:-44796131 Msa0253180:CDS
AAGAACAATTCATTCTAGAG+AGG 0.516878 2_2:-44796597 Msa0253180:CDS
ATGATTCAACCAGGTGACAT+TGG 0.524643 2_2:-44798301 Msa0253180:five_prime_UTR
GTTTCTTACTGAACGAGAAA+GGG 0.527402 2_2:+44798201 None:intergenic
CCACTACGTATGTACCCATC+CGG 0.530943 2_2:+44795566 None:intergenic
TGACAGTATGATGACAAAAC+CGG 0.531306 2_2:-44796042 Msa0253180:three_prime_UTR
TGCTACCGATGACTTTAGTC+CGG 0.531438 2_2:-44797624 Msa0253180:CDS
CAGAAACTTACATGCCTGCT+CGG 0.538038 2_2:+44796156 None:intergenic
GTCCTGATGCTCTGAGGCCG+AGG 0.551868 2_2:+44795932 None:intergenic
ATAAAATCAGAAATTAAGGC+CGG 0.557024 2_2:+44796023 None:intergenic
GCCAATAAAATCGGAGAGGG+TGG 0.565641 2_2:-44797602 Msa0253180:CDS
CATCTTGACCACAGTAGACA+TGG 0.572027 2_2:+44796088 None:intergenic
TGCGGTCTGGATGTATACTT+CGG 0.577061 2_2:+44795613 None:intergenic
ATCTTCGTATAATACATCCT+CGG 0.582976 2_2:-44795949 Msa0253180:three_prime_UTR
TGATGCTGTCTTGAAGAAGA+CGG 0.584599 2_2:+44798179 None:intergenic
CCGGATGGGTACATACGTAG+TGG 0.586607 2_2:-44795566 Msa0253180:three_prime_UTR
AGAACAATTCATTCTAGAGA+GGG 0.597759 2_2:-44796596 Msa0253180:intron
TGGCACCGAGTATGCAATAG+GGG 0.601183 2_2:-44796715 Msa0253180:CDS
ATGGGATCTTTACGAGCGAA+AGG 0.606057 2_2:-44796224 Msa0253180:CDS
GTGATCATATTGTGTAGTGA+AGG 0.623180 2_2:+44795878 None:intergenic
ATTGGCCGGACTAAAGTCAT+CGG 0.626267 2_2:+44797619 None:intergenic
CTCCTTGTGGAGATAGTCAG+CGG 0.634866 2_2:-44796652 Msa0253180:CDS
CCGGCCAATAAAATCGGAGA+GGG 0.636565 2_2:-44797605 Msa0253180:CDS
AAACCACTTCCAATGTCACC+TGG 0.644062 2_2:+44798292 None:intergenic
TTGTGTAGTGAAGGTTGAAG+TGG 0.652022 2_2:+44795887 None:intergenic
CCACAGTAGACATGGACGGT+CGG 0.654234 2_2:+44796096 None:intergenic
CTGTTGTGTAAATATGGACA+AGG 0.655886 2_2:-44795502 Msa0253180:three_prime_UTR
TCTCGAAGTGCATTTCTGGA+CGG 0.659178 2_2:-44795657 Msa0253180:three_prime_UTR
GGCACCGAGTATGCAATAGG+GGG 0.661136 2_2:-44796714 Msa0253180:CDS
GGGTGGGAAAAGTTCCTCAG+TGG 0.663539 2_2:-44795537 Msa0253180:three_prime_UTR
ATGTAAACATCAATTCACAA+AGG 0.674050 2_2:-44795852 Msa0253180:three_prime_UTR
TTCCGCTGACTATCTCCACA+AGG 0.675005 2_2:+44796650 None:intergenic
GGTAGTGTCCTGATGCTCTG+AGG 0.690372 2_2:+44795926 None:intergenic
GCACCGAGTATGCAATAGGG+GGG 0.692844 2_2:-44796713 Msa0253180:CDS
TCCGGCCAATAAAATCGGAG+AGG 0.695781 2_2:-44797606 Msa0253180:CDS
TTGACCACAGTAGACATGGA+CGG 0.697696 2_2:+44796092 None:intergenic
CCGACCGTCCATGTCTACTG+TGG 0.725374 2_2:-44796096 Msa0253180:CDS
AGTAGATACATTGCTAAACG+GGG 0.733078 2_2:-44796188 Msa0253180:CDS
AGACAGCATCAAGACAACGA+TGG 0.742510 2_2:-44798167 Msa0253180:intron
AGTACATTTAGCAACCACTG+AGG 0.791008 2_2:+44795523 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCGATTTTTTTTTTTTATT+TGG - chr2_2:44797961-44797980 Msa0253180:intron 10.0%
!!! TCGATTTTTTTTTTTTATTT+GGG - chr2_2:44797962-44797981 Msa0253180:intron 10.0%
!!! AAATGTTTTTTTTTTTTTTC+CGG - chr2_2:44798124-44798143 Msa0253180:intron 10.0%
!! AATCTTGTAAATTTGTAAAT+TGG - chr2_2:44795671-44795690 Msa0253180:three_prime_UTR 15.0%
!! AAAAACTATAATGATACAAT+TGG + chr2_2:44795896-44795915 None:intergenic 15.0%
!!! TAAAATTTGACTAATTTTCT+AGG - chr2_2:44795915-44795934 Msa0253180:three_prime_UTR 15.0%
!!! AAAATTTGACTAATTTTCTA+GGG - chr2_2:44795916-44795935 Msa0253180:three_prime_UTR 15.0%
!!! TTTTTACATTAATAGTGTAT+AGG - chr2_2:44797349-44797368 Msa0253180:intron 15.0%
!!! GGAATTAAATCATTTTTTAT+AGG - chr2_2:44797370-44797389 Msa0253180:intron 15.0%
!!! ATGAAGAATTTTTATATCAA+AGG + chr2_2:44797889-44797908 None:intergenic 15.0%
!! AAAAAAAAAATCGATAAGTA+TGG + chr2_2:44797956-44797975 None:intergenic 15.0%
!!! TTTTTTTTTTTATTTGGGTT+AGG - chr2_2:44797967-44797986 Msa0253180:intron 15.0%
!!! AAAAAAAACATTTTTTTGTG+CGG + chr2_2:44798117-44798136 None:intergenic 15.0%
!! AAACTTGTGTTTAAATACTA+CGG + chr2_2:44795698-44795717 None:intergenic 20.0%
!!! TTTTAATCTATAGCCTATAA+AGG - chr2_2:44795715-44795734 Msa0253180:intron 20.0%
!! ATGAAACCTTAATTGTATTA+CGG - chr2_2:44796204-44796223 Msa0253180:CDS 20.0%
!! GAAAATTTGGTTAAGTTATA+TGG - chr2_2:44796351-44796370 Msa0253180:intron 20.0%
!! AAAGATATGTGAATATGATT+GGG + chr2_2:44797154-44797173 None:intergenic 20.0%
!! AAAAGATATGTGAATATGAT+TGG + chr2_2:44797155-44797174 None:intergenic 20.0%
!!! AAATCATTTTTTATAGGCTT+TGG - chr2_2:44797376-44797395 Msa0253180:intron 20.0%
!!! TAAAAATTCTTCATTTTCTC+AGG - chr2_2:44797895-44797914 Msa0253180:intron 20.0%
!!! TTTTTTTTTTTTTTCCGGAT+GGG - chr2_2:44798129-44798148 Msa0253180:intron 20.0%
!! ATAATGTTAAGATTGCTGTT+AGG - chr2_2:44795590-44795609 Msa0253180:three_prime_UTR 25.0%
! GTGCATAGTTTATTATTAAG+TGG - chr2_2:44795617-44795636 Msa0253180:three_prime_UTR 25.0%
! TAGTTTATTATTAAGTGGTC+AGG - chr2_2:44795622-44795641 Msa0253180:three_prime_UTR 25.0%
!!! ACATTTCTTTGAAATACTGT+CGG - chr2_2:44796539-44796558 Msa0253180:intron 25.0%
! TTTATATCCCTATGAATGAT+AGG + chr2_2:44796780-44796799 None:intergenic 25.0%
! AGAATGAATTGTTCTTCTAT+TGG + chr2_2:44797108-44797127 None:intergenic 25.0%
! AAACTATAAAAAAGTGAAGC+AGG + chr2_2:44797230-44797249 None:intergenic 25.0%
!! GTTTAATTTCGATATACTGT+TGG - chr2_2:44797246-44797265 Msa0253180:intron 25.0%
! AACAAAGTTTAGATTAGAGT+AGG + chr2_2:44797456-44797475 None:intergenic 25.0%
! ATAAAATCAGAAATTAAGGC+CGG + chr2_2:44797689-44797708 None:intergenic 25.0%
! GTCCATAAAATCAGAAATTA+AGG + chr2_2:44797693-44797712 None:intergenic 25.0%
! ATGTAAACATCAATTCACAA+AGG - chr2_2:44797857-44797876 Msa0253180:intron 25.0%
!!! GTTTTTTTTTTTTTTCCGGA+TGG - chr2_2:44798128-44798147 Msa0253180:intron 25.0%
! AATGTACTGTTGTGTAAATA+TGG - chr2_2:44798201-44798220 Msa0253180:CDS 25.0%
AGATAACTAAGGAATTGCAA+TGG - chr2_2:44795567-44795586 Msa0253180:three_prime_UTR 30.0%
TACAGCTTACATTCCTTTAT+AGG + chr2_2:44795731-44795750 None:intergenic 30.0%
CGTGGTTATATTCTTGAATT+AGG - chr2_2:44795940-44795959 Msa0253180:three_prime_UTR 30.0%
GTACTTTGAACAGATATTTC+AGG - chr2_2:44796020-44796039 Msa0253180:three_prime_UTR 30.0%
TCTATACCTACAAAGAACTA+AGG - chr2_2:44796060-44796079 Msa0253180:three_prime_UTR 30.0%
AGGGAAAAAAAATAACGATC+AGG + chr2_2:44796168-44796187 None:intergenic 30.0%
CATACTCATCGATAAGAAAA+GGG + chr2_2:44796187-44796206 None:intergenic 30.0%
TCATACTCATCGATAAGAAA+AGG + chr2_2:44796188-44796207 None:intergenic 30.0%
! CAAATTTTCATGCTCTACTT+CGG + chr2_2:44796340-44796359 None:intergenic 30.0%
GTTGTGTCGAAAAAAATAAC+AGG - chr2_2:44796376-44796395 Msa0253180:intron 30.0%
GTGAAAGACTATTATTCTCA+AGG + chr2_2:44796419-44796438 None:intergenic 30.0%
CATATAAATCACAGAGAGTT+AGG + chr2_2:44796487-44796506 None:intergenic 30.0%
TAAAACAAATCCATGAAGCA+AGG + chr2_2:44796583-44796602 None:intergenic 30.0%
! AATTTTTACGTCTTCTCTTC+AGG - chr2_2:44796648-44796667 Msa0253180:CDS 30.0%
! AAGAACAATTCATTCTAGAG+AGG - chr2_2:44797112-44797131 Msa0253180:intron 30.0%
! AGAACAATTCATTCTAGAGA+GGG - chr2_2:44797113-44797132 Msa0253180:intron 30.0%
ATATGTGAATATGATTGGGA+AGG + chr2_2:44797150-44797169 None:intergenic 30.0%
AAACTTTGTTCTTACAGACA+TGG - chr2_2:44797466-44797485 Msa0253180:intron 30.0%
AACTTTGTTCTTACAGACAT+GGG - chr2_2:44797467-44797486 Msa0253180:intron 30.0%
CTAGTAGATACATTGCTAAA+CGG - chr2_2:44797519-44797538 Msa0253180:intron 30.0%
TAGTAGATACATTGCTAAAC+GGG - chr2_2:44797520-44797539 Msa0253180:intron 30.0%
! GGCCTTAATTTCTGATTTTA+TGG - chr2_2:44797688-44797707 Msa0253180:intron 30.0%
CTTGTTTATGTCTAACTTTG+AGG + chr2_2:44797715-44797734 None:intergenic 30.0%
ATCTTCGTATAATACATCCT+CGG - chr2_2:44797760-44797779 Msa0253180:intron 30.0%
!!! AGATGTTTTTTAGCACTATG+GGG + chr2_2:44798035-44798054 None:intergenic 30.0%
!!! GAGATGTTTTTTAGCACTAT+GGG + chr2_2:44798036-44798055 None:intergenic 30.0%
GACAAAGACAAATTATGAGA+AGG + chr2_2:44798297-44798316 None:intergenic 30.0%
GTTTCTTACTGAACGAGAAA+GGG + chr2_2:44795511-44795530 None:intergenic 35.0%
CAACGATGGTAAGATAACTA+AGG - chr2_2:44795556-44795575 Msa0253180:three_prime_UTR 35.0%
! GTTAAGATTGCTGTTAGGTA+TGG - chr2_2:44795595-44795614 Msa0253180:three_prime_UTR 35.0%
CGAGATTTGATCATAGAGTT+AGG + chr2_2:44795788-44795807 None:intergenic 35.0%
!! TATCCGTTTACTTTTGAACC+AGG - chr2_2:44795831-44795850 Msa0253180:three_prime_UTR 35.0%
!! ATCCGTTTACTTTTGAACCA+GGG - chr2_2:44795832-44795851 Msa0253180:three_prime_UTR 35.0%
! AGCATTCCTTAGTTCTTTGT+AGG + chr2_2:44796069-44796088 None:intergenic 35.0%
TTTAGTCCGGCCAATAAAAT+CGG - chr2_2:44796098-44796117 Msa0253180:CDS 35.0%
ACCTTAATTGTATTACGGTG+TGG - chr2_2:44796209-44796228 Msa0253180:CDS 35.0%
TCCACACCGTAATACAATTA+AGG + chr2_2:44796213-44796232 None:intergenic 35.0%
CGCTGCTATAAAAGTTCTTT+CGG - chr2_2:44796263-44796282 Msa0253180:intron 35.0%
CGAAGTAGAGCATGAAAATT+TGG - chr2_2:44796338-44796357 Msa0253180:intron 35.0%
AGTCTTTCACAAACACTTCT+AGG - chr2_2:44796429-44796448 Msa0253180:intron 35.0%
GTGCTAGTATCTACTTTGAT+TGG - chr2_2:44796685-44796704 Msa0253180:CDS 35.0%
ATATCCCTATGAATGATAGG+AGG + chr2_2:44796777-44796796 None:intergenic 35.0%
AGACCAAAATCCGAAATCTT+GGG + chr2_2:44796837-44796856 None:intergenic 35.0%
AAGACCAAAATCCGAAATCT+TGG + chr2_2:44796838-44796857 None:intergenic 35.0%
GTCAGAAGTTTAATGATGCA+TGG - chr2_2:44796953-44796972 Msa0253180:intron 35.0%
GGTCTATTTCAGAGGTTATT+TGG - chr2_2:44796974-44796993 Msa0253180:intron 35.0%
! AAGGCAGACATTTACAGTTT+TGG - chr2_2:44797030-44797049 Msa0253180:intron 35.0%
! CTTTTACATAGAGATGAGCA+TGG + chr2_2:44797183-44797202 None:intergenic 35.0%
AGCAGGTAATTCTGGATATA+GGG + chr2_2:44797213-44797232 None:intergenic 35.0%
AAGCAGGTAATTCTGGATAT+AGG + chr2_2:44797214-44797233 None:intergenic 35.0%
AAAAGTGAAGCAGGTAATTC+TGG + chr2_2:44797221-44797240 None:intergenic 35.0%
AGTAGATACATTGCTAAACG+GGG - chr2_2:44797521-44797540 Msa0253180:intron 35.0%
! GCATGTAAGTTTCTGAAGAT+TGG - chr2_2:44797561-44797580 Msa0253180:intron 35.0%
TGACAGTATGATGACAAAAC+CGG - chr2_2:44797667-44797686 Msa0253180:CDS 35.0%
CGAAGATGAAGTCTTCATAT+CGG + chr2_2:44797747-44797766 None:intergenic 35.0%
GTGATCATATTGTGTAGTGA+AGG + chr2_2:44797834-44797853 None:intergenic 35.0%
!!! ATGTTTTTTAGCACTATGGG+GGG + chr2_2:44798033-44798052 None:intergenic 35.0%
!!! GATGTTTTTTAGCACTATGG+GGG + chr2_2:44798034-44798053 None:intergenic 35.0%
!!! CGAGATGTTTTTTAGCACTA+TGG + chr2_2:44798037-44798056 None:intergenic 35.0%
!!! AAACATTTTTTTGTGCGGTC+TGG + chr2_2:44798112-44798131 None:intergenic 35.0%
CTGTTGTGTAAATATGGACA+AGG - chr2_2:44798207-44798226 Msa0253180:CDS 35.0%
GGTTTCTTACTGAACGAGAA+AGG + chr2_2:44795512-44795531 None:intergenic 40.0%
TGATGCTGTCTTGAAGAAGA+CGG + chr2_2:44795533-44795552 None:intergenic 40.0%
GTTAGAGACGTAGGTACAAT+TGG - chr2_2:44795762-44795781 Msa0253180:intron 40.0%
AGCCCTGGTTCAAAAGTAAA+CGG + chr2_2:44795837-44795856 None:intergenic 40.0%
AATTGTTTGAACAACAGCCC+TGG + chr2_2:44795852-44795871 None:intergenic 40.0%
!! TGACTAATTTTCTAGGGACG+TGG - chr2_2:44795922-44795941 Msa0253180:three_prime_UTR 40.0%
!!! TGGTTTTGGTTCTGTCTACA+TGG - chr2_2:44796127-44796146 Msa0253180:CDS 40.0%
TATTACGGTGTGGATGATCA+GGG - chr2_2:44796219-44796238 Msa0253180:CDS 40.0%
GATGATCAGGGACTACTTAA+AGG - chr2_2:44796231-44796250 Msa0253180:intron 40.0%
TTTCGGCTGAATCAAGACAA+GGG - chr2_2:44796280-44796299 Msa0253180:intron 40.0%
TAAAAGAACGCCTTGCTTCA+TGG - chr2_2:44796570-44796589 Msa0253180:intron 40.0%
! TTTTACGTCTTCTCTTCAGG+CGG - chr2_2:44796651-44796670 Msa0253180:CDS 40.0%
TCTACTTTGATTGGCGAACA+CGG - chr2_2:44796694-44796713 Msa0253180:CDS 40.0%
CCTGACTTCTTCATGAAGAA+AGG + chr2_2:44796754-44796773 None:intergenic 40.0%
CCTTTCTTCATGAAGAAGTC+AGG - chr2_2:44796751-44796770 Msa0253180:intron 40.0%
AGACCTTACACCCAAGATTT+CGG - chr2_2:44796824-44796843 Msa0253180:intron 40.0%
AATCCGAAATCTTGGGTGTA+AGG + chr2_2:44796830-44796849 None:intergenic 40.0%
! ACACCCAAGATTTCGGATTT+TGG - chr2_2:44796831-44796850 Msa0253180:intron 40.0%
TGATGCATGGTCTATTTCAG+AGG - chr2_2:44796966-44796985 Msa0253180:intron 40.0%
AGGTAATTCTGGATATAGGG+AGG + chr2_2:44797210-44797229 None:intergenic 40.0%
TACGAGCGAAAGGAATTAGT+TGG - chr2_2:44797495-44797514 Msa0253180:intron 40.0%
!! GTCATCGACTTTCATTTCTC+CGG + chr2_2:44797651-44797670 None:intergenic 40.0%
AGTGGCAGCTGATGATATAA+TGG + chr2_2:44797807-44797826 None:intergenic 40.0%
TTGTGTAGTGAAGGTTGAAG+TGG + chr2_2:44797825-44797844 None:intergenic 40.0%
AACATCTCGAAGTGCATTTC+TGG - chr2_2:44798048-44798067 Msa0253180:intron 40.0%
! GGTACATACGTAGTGGTTTT+GGG - chr2_2:44798150-44798169 Msa0253180:intron 40.0%
!! GTACATACGTAGTGGTTTTG+GGG - chr2_2:44798151-44798170 Msa0253180:intron 40.0%
!!! TACATACGTAGTGGTTTTGG+GGG - chr2_2:44798152-44798171 Msa0253180:intron 40.0%
AGTACATTTAGCAACCACTG+AGG + chr2_2:44798189-44798208 None:intergenic 40.0%
AAACCACTTCCAATGTCACC+TGG + chr2_2:44795420-44795439 None:intergenic 45.0%
ACTGAACGAGAAAGGGAAAC+AGG + chr2_2:44795504-44795523 None:intergenic 45.0%
AGACAGCATCAAGACAACGA+TGG - chr2_2:44795542-44795561 Msa0253180:three_prime_UTR 45.0%
TGCTACCGATGACTTTAGTC+CGG - chr2_2:44796085-44796104 Msa0253180:CDS 45.0%
ATTGGCCGGACTAAAGTCAT+CGG + chr2_2:44796093-44796112 None:intergenic 45.0%
! ACCACCCTCTCCGATTTTAT+TGG + chr2_2:44796111-44796130 None:intergenic 45.0%
! AAAATCGGAGAGGGTGGTTT+TGG - chr2_2:44796113-44796132 Msa0253180:CDS 45.0%
GTATTACGGTGTGGATGATC+AGG - chr2_2:44796218-44796237 Msa0253180:CDS 45.0%
GATCAGGGACTACTTAAAGG+TGG - chr2_2:44796234-44796253 Msa0253180:intron 45.0%
CTTTCGGCTGAATCAAGACA+AGG - chr2_2:44796279-44796298 Msa0253180:intron 45.0%
TTCGGCTGAATCAAGACAAG+GGG - chr2_2:44796281-44796300 Msa0253180:intron 45.0%
! ACACGGTGTCGAATATGCAT+TGG - chr2_2:44796711-44796730 Msa0253180:CDS 45.0%
TCAGGCCTCCTATCATTCAT+AGG - chr2_2:44796769-44796788 Msa0253180:intron 45.0%
CAGGCCTCCTATCATTCATA+GGG - chr2_2:44796770-44796789 Msa0253180:intron 45.0%
TGGGTGTAAGGTCTTTGTCA+AGG + chr2_2:44796818-44796837 None:intergenic 45.0%
! TTTGGCACCGAGTATGCAAT+AGG - chr2_2:44796992-44797011 Msa0253180:intron 45.0%
! TTGGCACCGAGTATGCAATA+GGG - chr2_2:44796993-44797012 Msa0253180:intron 45.0%
!!! CAGTTTTGGTGTTCTCCTTG+TGG - chr2_2:44797044-44797063 Msa0253180:intron 45.0%
ATGGGATCTTTACGAGCGAA+AGG - chr2_2:44797485-44797504 Msa0253180:intron 45.0%
CAGAAACTTACATGCCTGCT+CGG + chr2_2:44797556-44797575 None:intergenic 45.0%
GAGATTCCTGAGTGCAAAGA+AGG + chr2_2:44797587-44797606 None:intergenic 45.0%
TTGACCACAGTAGACATGGA+CGG + chr2_2:44797620-44797639 None:intergenic 45.0%
CATCTTGACCACAGTAGACA+TGG + chr2_2:44797624-44797643 None:intergenic 45.0%
ACTGTGGTCAAGATGCTAAC+CGG - chr2_2:44797629-44797648 Msa0253180:CDS 45.0%
! TTGGGTTAGGCTTCGTGTTT+CGG - chr2_2:44797980-44797999 Msa0253180:intron 45.0%
!!! TTTTTTAGCACTATGGGGGG+TGG + chr2_2:44798030-44798049 None:intergenic 45.0%
! TCTCGAAGTGCATTTCTGGA+CGG - chr2_2:44798052-44798071 Msa0253180:intron 45.0%
TGCGGTCTGGATGTATACTT+CGG + chr2_2:44798099-44798118 None:intergenic 45.0%
! GGGTACATACGTAGTGGTTT+TGG - chr2_2:44798149-44798168 Msa0253180:intron 45.0%
CAACCAGGTGACATTGGAAG+TGG - chr2_2:44795414-44795433 Msa0253180:three_prime_UTR 50.0%
TCGCTCGAGCTGAAGATTCA+AGG - chr2_2:44795645-44795664 Msa0253180:three_prime_UTR 50.0%
CGCTCGAGCTGAAGATTCAA+GGG - chr2_2:44795646-44795665 Msa0253180:three_prime_UTR 50.0%
CTACGTCTCTAACTGCTGAG+TGG + chr2_2:44795755-44795774 None:intergenic 50.0%
CACTCAGCAGTTAGAGACGT+AGG - chr2_2:44795753-44795772 Msa0253180:intron 50.0%
TCCGGCCAATAAAATCGGAG+AGG - chr2_2:44796103-44796122 Msa0253180:CDS 50.0%
! CCCTCTCCGATTTTATTGGC+CGG + chr2_2:44796107-44796126 None:intergenic 50.0%
CCGGCCAATAAAATCGGAGA+GGG - chr2_2:44796104-44796123 Msa0253180:CDS 50.0%
GCCAATAAAATCGGAGAGGG+TGG - chr2_2:44796107-44796126 Msa0253180:CDS 50.0%
! ATATGCATTGGCGTTGCACG+TGG - chr2_2:44796723-44796742 Msa0253180:CDS 50.0%
! TATGCATTGGCGTTGCACGT+GGG - chr2_2:44796724-44796743 Msa0253180:CDS 50.0%
CTGACATGAGTCGCATTTGC+TGG + chr2_2:44796873-44796892 None:intergenic 50.0%
! TGGCACCGAGTATGCAATAG+GGG - chr2_2:44796994-44797013 Msa0253180:intron 50.0%
CTCCTTGTGGAGATAGTCAG+CGG - chr2_2:44797057-44797076 Msa0253180:intron 50.0%
! TTCCGCTGACTATCTCCACA+AGG + chr2_2:44797062-44797081 None:intergenic 50.0%
!! GATTGGCCTTCTTTGCACTC+AGG - chr2_2:44797578-44797597 Msa0253180:intron 50.0%
CCACTACGTATGTACCCATC+CGG + chr2_2:44798146-44798165 None:intergenic 50.0%
!!! ATACGTAGTGGTTTTGGGGG+TGG - chr2_2:44798155-44798174 Msa0253180:intron 50.0%
!!! TACGTAGTGGTTTTGGGGGT+GGG - chr2_2:44798156-44798175 Msa0253180:intron 50.0%
TACGTCTTCTCTTCAGGCGG+AGG - chr2_2:44796654-44796673 Msa0253180:CDS 55.0%
! GGCACCGAGTATGCAATAGG+GGG - chr2_2:44796995-44797014 Msa0253180:intron 55.0%
GCACCGAGTATGCAATAGGG+GGG - chr2_2:44796996-44797015 Msa0253180:intron 55.0%
GCTCCCCCCTATTGCATACT+CGG + chr2_2:44797002-44797021 None:intergenic 55.0%
TAGGGGGGAGCTTACACGAA+AGG - chr2_2:44797011-44797030 Msa0253180:intron 55.0%
CCACAGTAGACATGGACGGT+CGG + chr2_2:44797616-44797635 None:intergenic 55.0%
GGTAGTGTCCTGATGCTCTG+AGG + chr2_2:44797786-44797805 None:intergenic 55.0%
CCGGATGGGTACATACGTAG+TGG - chr2_2:44798143-44798162 Msa0253180:intron 55.0%
GGGTGGGAAAAGTTCCTCAG+TGG - chr2_2:44798172-44798191 Msa0253180:CDS 55.0%
CATGTCAGCACACGCGTAGC+AGG - chr2_2:44796885-44796904 Msa0253180:intron 60.0%
CGGGGAATTTGATGCCGAGC+AGG - chr2_2:44797539-44797558 Msa0253180:intron 60.0%
! AGACATGGACGGTCGGCTCT+TGG + chr2_2:44797609-44797628 None:intergenic 60.0%
CCGACCGTCCATGTCTACTG+TGG - chr2_2:44797613-44797632 Msa0253180:CDS 60.0%
! ATCCTCGGCCTCAGAGCATC+AGG - chr2_2:44797775-44797794 Msa0253180:intron 60.0%
!! GTCCTGATGCTCTGAGGCCG+AGG + chr2_2:44797780-44797799 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 44795411 44798320 44795411 ID=Msa0253180;Name=Msa0253180
chr2_2 mRNA 44795411 44798320 44795411 ID=Msa0253180-mRNA-1;Parent=Msa0253180;Name=Msa0253180-mRNA-1;_AED=0.17;_eAED=0.17;_QI=86|0.75|0.8|1|0.5|0.4|5|635|164
chr2_2 exon 44795411 44795728 44795411 ID=Msa0253180-mRNA-1:exon:13818;Parent=Msa0253180-mRNA-1
chr2_2 exon 44795767 44796248 44795767 ID=Msa0253180-mRNA-1:exon:13817;Parent=Msa0253180-mRNA-1
chr2_2 exon 44796597 44796743 44796597 ID=Msa0253180-mRNA-1:exon:13816;Parent=Msa0253180-mRNA-1
chr2_2 exon 44797583 44797698 44797583 ID=Msa0253180-mRNA-1:exon:13815;Parent=Msa0253180-mRNA-1
chr2_2 exon 44798168 44798320 44798168 ID=Msa0253180-mRNA-1:exon:13814;Parent=Msa0253180-mRNA-1
chr2_2 five_prime_UTR 44798235 44798320 44798235 ID=Msa0253180-mRNA-1:five_prime_utr;Parent=Msa0253180-mRNA-1
chr2_2 CDS 44798168 44798234 44798168 ID=Msa0253180-mRNA-1:cds;Parent=Msa0253180-mRNA-1
chr2_2 CDS 44797583 44797698 44797583 ID=Msa0253180-mRNA-1:cds;Parent=Msa0253180-mRNA-1
chr2_2 CDS 44796597 44796743 44796597 ID=Msa0253180-mRNA-1:cds;Parent=Msa0253180-mRNA-1
chr2_2 CDS 44796084 44796248 44796084 ID=Msa0253180-mRNA-1:cds;Parent=Msa0253180-mRNA-1
chr2_2 three_prime_UTR 44795767 44796083 44795767 ID=Msa0253180-mRNA-1:three_prime_utr;Parent=Msa0253180-mRNA-1
chr2_2 three_prime_UTR 44795411 44795728 44795411 ID=Msa0253180-mRNA-1:three_prime_utr;Parent=Msa0253180-mRNA-1
Gene Sequence

>Msa0253180

ATGACCTGTTTCCCTTTCTCGTTCAGTAAGAAACCGTCTTCTTCAAGACAGCATCAAGACAACGATGATATTTCAGGCATTCACAATGTTAAAATCTATACCTACAAAGAACTAAGGAATGCTACCGATGACTTTAGTCCGGCCAATAAAATCGGAGAGGGTGGTTTTGGTTCTGTCTACATGGTTATTTGGCACCGAGTATGCAATAGGGGGGAGCTTACACGAAAGGCAGACATTTACAGTTTTGGTGTTCTCCTTGTGGAGATAGTCAGCGGAAGATGTAACACAAATTCGCGACTGCCAATAGAAGAACAATTCATTCTAGAGAGGACATGGGATCTTTACGAGCGAAAGGAATTAGTTGGACTAGTAGATACATTGCTAAACGGGGAATTTGATGCCGAGCAGGCATGTAAGTTTCTGAAGATTGGCCTTCTTTGCACTCAGGAATCTCCAAGAGCCGACCGTCCATGTCTACTGTGGTCAAGATGCTAA
Protein sequence

>Msa0253180

MTCFPFSFSKKPSSSRQHQDNDDISGIHNVKIYTYKELRNATDDFSPANKIGEGGFGSVYMVIWHRVCNRGELTRKADIYSFGVLLVEIVSGRCNTNSRLPIEEQFILERTWDLYERKELVGLVDTLLNGEFDAEQACKFLKIGLLCTQESPRADRPCLLWSRC*