AlfalfaGEDB | Msa0763080

M. sativa cultivar zhongmu-4 / Msa0763080

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008134 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030957 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0763080	sp\|O49840\|PBL3_ARATH	71.127	426	105	4	1	409	1	425	0.0	607

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0763080	Q2HTW4	99.024	410	4	0	1	410	1	410	0.0	839

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003560	Msa0763080	0.813148	2.963509e-51	-8.615850e-47
Msa0038020	Msa0763080	0.849552	3.006203e-60	-8.615850e-47
Msa0076700	Msa0763080	0.806715	7.190130e-50	-8.615850e-47
Msa0085720	Msa0763080	0.889373	2.567868e-73	-8.615850e-47
Msa0108850	Msa0763080	0.827337	1.653903e-54	-8.615850e-47
Msa0124550	Msa0763080	0.830027	3.699284e-55	-8.615850e-47
Msa0130520	Msa0763080	0.812246	4.668127e-51	-8.615850e-47
Msa0132240	Msa0763080	0.838790	2.331999e-57	-8.615850e-47
Msa0140170	Msa0763080	0.816065	6.700290e-52	-8.615850e-47
Msa0151950	Msa0763080	0.823817	1.129146e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0763080	MtrunA17_Chr5g0416311	99.024	410	4	0	1	410	1	410	0.0	839
Msa0763080	MtrunA17_Chr4g0060531	65.039	389	126	5	1	381	1	387	0.0	528
Msa0763080	MtrunA17_Chr2g0328171	59.596	396	142	3	1	394	1	380	8.95e-174	491
Msa0763080	MtrunA17_Chr7g0238671	63.415	369	130	2	1	367	1	366	1.90e-169	480
Msa0763080	MtrunA17_Chr5g0435211	60.440	364	139	3	1	361	1	362	1.32e-165	471
Msa0763080	MtrunA17_Chr5g0441021	54.567	427	157	6	1	410	1	407	9.29e-161	458
Msa0763080	MtrunA17_Chr7g0216551	58.424	368	145	2	1	360	41	408	8.17e-155	446
Msa0763080	MtrunA17_Chr3g0093381	59.497	358	143	2	12	367	43	400	5.21e-154	441
Msa0763080	MtrunA17_Chr5g0416011	59.401	367	136	4	1	356	52	416	1.92e-151	437
Msa0763080	MtrunA17_Chr3g0111631	58.402	363	145	4	1	360	3	362	3.12e-151	434

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0763080	AT2G02800.1	71.127	426	105	4	1	409	1	425	0.0	607
Msa0763080	AT2G02800.2	71.127	426	105	4	1	409	1	425	0.0	607
Msa0763080	AT1G14370.1	70.423	426	108	5	1	409	1	425	0.0	590
Msa0763080	AT1G69790.1	71.739	368	94	3	1	360	1	366	0.0	544
Msa0763080	AT1G26970.1	64.833	418	131	6	1	409	1	411	0.0	534
Msa0763080	AT1G69790.2	71.508	358	96	3	5	360	2	355	0.0	531
Msa0763080	AT1G07570.1	64.305	367	121	3	1	365	1	359	1.18e-173	491
Msa0763080	AT1G07570.5	64.305	367	121	3	1	365	1	359	1.18e-173	491
Msa0763080	AT1G07570.2	64.305	367	121	3	1	365	1	359	1.18e-173	491
Msa0763080	AT5G02290.2	60.104	386	144	2	1	384	1	378	2.82e-171	484

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Find 208 sgRNAs with CRISPR-Local

Find 532 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTGGAATGAAGAACT+TGG	0.529480	5_2:+21779768	Msa0763080:three_prime_UTR
AAAATGAACAATACTCCGTT+AGG	0.472779	5_2:+21779886	Msa0763080:three_prime_UTR
AAACGTGGAATGAAGAACTT+GGG	0.613512	5_2:+21779769	Msa0763080:three_prime_UTR
AAAGCTGTATACATCACTCT+TGG	0.548445	5_2:-21778777	None:intergenic
AAATGAACATGTCATGTGAA+AGG	0.582141	5_2:-21772529	None:intergenic
AACAACGGACAGCCGTACAC+GGG	0.617103	5_2:-21780051	None:intergenic
AACAATGTACTCCATTAAGT+GGG	0.570395	5_2:+21779399	Msa0763080:three_prime_UTR
AACCACTGTCTTGGTCAATA+AGG	0.357178	5_2:+21778077	Msa0763080:CDS
AACGGACAGCCGTACACGGG+TGG	0.558049	5_2:-21780048	None:intergenic
AACTTACCAACCACTCTTTA+TGG	0.153876	5_2:-21777415	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAGTACACAACAGAAAAAG+AGG	-	chr5_2:21772648-21772667	None:intergenic	30.0%
TGTACTTATAACAGTTGAAG+TGG	+	chr5_2:21772660-21772679	Msa0763080:five_prime_UTR	30.0%
TTGTGATCATTATGTTCTTG+TGG	+	chr5_2:21772916-21772935	Msa0763080:intron	30.0%
TTTGGCAAAGAGAAGAAATT+AGG	+	chr5_2:21772963-21772982	Msa0763080:intron	30.0%
AGTAGATTTAAGAGGTTGTT+TGG	+	chr5_2:21773040-21773059	Msa0763080:intron	30.0%
AAATAGTGTAAGATATGACC+AGG	+	chr5_2:21773469-21773488	Msa0763080:intron	30.0%
AATAGTGTAAGATATGACCA+GGG	+	chr5_2:21773470-21773489	Msa0763080:intron	30.0%
TTTGAAAAATGCTTTCACGA+AGG	+	chr5_2:21774059-21774078	Msa0763080:intron	30.0%
GCACTCTTAGTAAATCTAAA+AGG	-	chr5_2:21774271-21774290	None:intergenic	30.0%
AAGAGTTTATTCCTTGTAGT+AGG	-	chr5_2:21774309-21774328	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	21772532	21780597	21772532	ID=Msa0763080;Name=Msa0763080
chr5_2	mRNA	21772532	21780597	21772532	ID=Msa0763080-mRNA-1;Parent=Msa0763080;Name=Msa0763080-mRNA-1;_AED=0.08;_eAED=0.08;_QI=196\|1\|1\|1\|1\|1\|6\|1381\|410
chr5_2	exon	21772532	21772791	21772532	ID=Msa0763080-mRNA-1:exon:9023;Parent=Msa0763080-mRNA-1
chr5_2	exon	21777111	21777430	21777111	ID=Msa0763080-mRNA-1:exon:9024;Parent=Msa0763080-mRNA-1
chr5_2	exon	21777802	21777937	21777802	ID=Msa0763080-mRNA-1:exon:9025;Parent=Msa0763080-mRNA-1
chr5_2	exon	21778068	21778204	21778068	ID=Msa0763080-mRNA-1:exon:9026;Parent=Msa0763080-mRNA-1
chr5_2	exon	21778300	21778423	21778300	ID=Msa0763080-mRNA-1:exon:9027;Parent=Msa0763080-mRNA-1
chr5_2	exon	21778765	21780597	21778765	ID=Msa0763080-mRNA-1:exon:9028;Parent=Msa0763080-mRNA-1
chr5_2	five_prime_UTR	21772532	21772727	21772532	ID=Msa0763080-mRNA-1:five_prime_utr;Parent=Msa0763080-mRNA-1
chr5_2	CDS	21772728	21772791	21772728	ID=Msa0763080-mRNA-1:cds;Parent=Msa0763080-mRNA-1
chr5_2	CDS	21777111	21777430	21777111	ID=Msa0763080-mRNA-1:cds;Parent=Msa0763080-mRNA-1
chr5_2	CDS	21777802	21777937	21777802	ID=Msa0763080-mRNA-1:cds;Parent=Msa0763080-mRNA-1
chr5_2	CDS	21778068	21778204	21778068	ID=Msa0763080-mRNA-1:cds;Parent=Msa0763080-mRNA-1
chr5_2	CDS	21778300	21778423	21778300	ID=Msa0763080-mRNA-1:cds;Parent=Msa0763080-mRNA-1
chr5_2	CDS	21778765	21779216	21778765	ID=Msa0763080-mRNA-1:cds;Parent=Msa0763080-mRNA-1
chr5_2	three_prime_UTR	21779217	21780597	21779217	ID=Msa0763080-mRNA-1:three_prime_utr;Parent=Msa0763080-mRNA-1

Gene Sequence

>Msa0763080

ATGGGTAATTGTCTTGATTCTTCTGCTAAAGTTGATGCTGCTCAAAGCTCTAGATCCACTTCTGGAACCTCAAAAACTACTCCTTCTAGTTTAACAATTCCATCATACAGTGACAAAAGCAATTCCTCAAGTCTTCTTCCTACACCAAGGTCTGAGGGTGAAATCTTATCTTCTCCCAATCTTAAAGCATTCTCATTCAATGAGCTGAAAAATGCCACAAGGAATTTTCGTCCCGATAGTCTTCTAGGCGAAGGCGGATTTGGTCATGTTTATAAGGGATGGATCGACGAACACACATTCACAGCTGCAAAACCTGGATCAGGAATGGTTGTTGCTGTTAAAAGACTTAAGCCTGAAGGTTTTCAAGGCCATAAAGAGTGGTTGACTGAGGTTAACTACCTTGGACAACTTCACCATCCTAATCTAGTCAAATTAATTGGTTACTGCTTGGAAGGAGAGAACAGGCTTTTGGTATATGAATTTATGCCTAAAGGGAGCTTAGAGAACCATCTTTTTAGACGAGGACCACAACCACTGTCTTGGTCAATAAGGATGAAAGTAGCTATCGGTGCTGCTAGAGGTCTCTCGTTTCTTCACAATGCAAAATCTCAAGTCATATACCGTGATTTCAAAGCTTCAAACATCCTTTTAGACGCGGAGTTTAATTCTAAACTCTCCGATTTTGGCTTGGCTAAAGCGGGTCCTACCGGTGATAGAACTCATGTGTCTACTCAAGTAGTCGGTACTCAAGGATATGCAGCACCTGAATATGTTGCAACTGGTCGGCTAACGGCCAAGAGTGATGTATACAGCTTTGGAGTTGTTATGCTTGAACTTTTATCTGGAAGACGAGCCGTTGATAAAACAATAGCTGGCGTGGACCAGAATCTAGTAGACTGGGCAAAACCATATTTAGGCGATAAAAGAAGACTATTTCGGATCATGGATTCAAAATTAGAAGGTCAATATCCACAAAAAGGAGCATTCATGGCTGCTACACTTGCACTACAATGCCTTAACAGAGAAGCTAAGGCAAGGCCTTCAATGACAGAGGTTTTAGCAACTCTAGAACAGATAGAAGCTCCTAAACAAGCATCACGGAATTCCTTATCGGAACATCATAGATTCCATGCTCCGGTCAGAAGGTCTCCTGCACGAAACCGAACGCCTTCAAATGTTACTCCTACTGCATCGCCATTGCCATCTGTCCGGCAATCTCCTCGTGCGCACTAG

Protein sequence

>Msa0763080

MGNCLDSSAKVDAAQSSRSTSGTSKTTPSSLTIPSYSDKSNSSSLLPTPRSEGEILSSPNLKAFSFNELKNATRNFRPDSLLGEGGFGHVYKGWIDEHTFTAAKPGSGMVVAVKRLKPEGFQGHKEWLTEVNYLGQLHHPNLVKLIGYCLEGENRLLVYEFMPKGSLENHLFRRGPQPLSWSIRMKVAIGAARGLSFLHNAKSQVIYRDFKASNILLDAEFNSKLSDFGLAKAGPTGDRTHVSTQVVGTQGYAAPEYVATGRLTAKSDVYSFGVVMLELLSGRRAVDKTIAGVDQNLVDWAKPYLGDKRRLFRIMDSKLEGQYPQKGAFMAATLALQCLNREAKARPSMTEVLATLEQIEAPKQASRNSLSEHHRFHAPVRRSPARNRTPSNVTPTASPLPSVRQSPRAH*