AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0354660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0354660 MtrunA17_Chr3g0090341 98.425 127 2 0 1 127 1 127 6.09e-89 253
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0354660 AT5G49550.1 67.593 108 35 0 14 121 5 112 3.91e-53 163

Find 84 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGGTTTATACCGTTTATT+TGG 0.152595 3_1:+26650355 Msa0354660:three_prime_UTR
TCTCTCAATTTGTATCATTT+TGG 0.188725 3_1:+26650332 Msa0354660:three_prime_UTR
GGGTTTGGCGATTTGGCTTC+AGG 0.236775 3_1:+26650041 Msa0354660:CDS
GTATAACGGGTTTGGCGATT+TGG 0.304459 3_1:+26650034 Msa0354660:CDS
CGATTTGGCTTCAGGGTTAA+GGG 0.313912 3_1:+26650049 Msa0354660:CDS
GTTCATCACGCTTCTGATCC+TGG 0.319473 3_1:-26649763 None:intergenic
GCGATTTGGCTTCAGGGTTA+AGG 0.320780 3_1:+26650048 Msa0354660:CDS
AGAGGCTGTTTCCAGGAAAA+TGG 0.323147 3_1:-26650661 None:intergenic
CCCAACCTTTCCTTGTGAAT+TGG 0.325145 3_1:+26649695 Msa0354660:five_prime_UTR
CAGTGTACAAATTGAGGAAA+TGG 0.345104 3_1:+26650260 Msa0354660:three_prime_UTR
GAGAGAGATAAAGATGTTGC+AGG 0.346615 3_1:-26650611 None:intergenic
TAATAATCATCTAGAGCTAT+TGG 0.354512 3_1:+26649983 Msa0354660:CDS
CAAATGGTGGGACCCCTTCC+CGG 0.367038 3_1:+26650720 Msa0354660:three_prime_UTR
ACGATCTGGTCACAACAAAA+TGG 0.370600 3_1:+26650425 Msa0354660:three_prime_UTR
CGGGAAGGGGTCCCACCATT+TGG 0.381455 3_1:-26650719 None:intergenic
TTACACAAGAGGCTGTTTCC+AGG 0.382901 3_1:-26650668 None:intergenic
CATGATCAAATCAGAGCTTC+AGG 0.388388 3_1:+26649826 Msa0354660:CDS
CCGGTTTATACCGTTTATTT+GGG 0.391856 3_1:+26650356 Msa0354660:three_prime_UTR
AGCCGATGCGGCCTCAATTA+CGG 0.415660 3_1:-26650479 None:intergenic
GTAAACATAAGGAGATGAAA+AGG 0.418422 3_1:-26650637 None:intergenic
TTCAGGGTTAAGGGTGTTTG+TGG 0.420422 3_1:+26650058 Msa0354660:CDS
TGATAAATATGTGGCTTTGT+TGG 0.429542 3_1:+26650181 Msa0354660:CDS
TTCAGTCACTTGCTTCTCAA+TGG 0.438225 3_1:-26650135 None:intergenic
GCATACGCAGGGTCCGGGAA+GGG 0.438816 3_1:-26650733 None:intergenic
TTCATCACGCTTCTGATCCT+GGG 0.439268 3_1:-26649762 None:intergenic
ATCTTTCTTTATATTAGCCA+TGG 0.439568 3_1:-26649735 None:intergenic
CATACGCAGGGTCCGGGAAG+GGG 0.441791 3_1:-26650732 None:intergenic
GCTCCCGCATACGCAGGGTC+CGG 0.442051 3_1:-26650739 None:intergenic
CCCAAATAAACGGTATAAAC+CGG 0.443820 3_1:-26650356 None:intergenic
AATGGTCCGCGACCACGATC+TGG 0.449640 3_1:+26650411 Msa0354660:three_prime_UTR
TAAGTAAATTAGCAGGGTGC+AGG 0.450218 3_1:-26650305 None:intergenic
GCAGAAGAGTATAACGGGTT+TGG 0.452290 3_1:+26650026 Msa0354660:CDS
GATAAAGTGTAATCCAAAAC+AGG 0.461299 3_1:+26647029 Msa0354660:five_prime_UTR
CCACGACAGAGGGGAGTTTG+AGG 0.463554 3_1:-26650380 None:intergenic
TTGGACCATAAAACAACTCT+TGG 0.464466 3_1:+26649642 Msa0354660:five_prime_UTR
TTCTTTATATTAGCCATGGT+TGG 0.465048 3_1:-26649731 None:intergenic
AGGAAAATGGAGTAAACATA+AGG 0.465793 3_1:-26650648 None:intergenic
GAGTAGCAGAAGAGTATAAC+GGG 0.470456 3_1:+26650021 Msa0354660:CDS
ACCAATTCACAAGGAAAGGT+TGG 0.471373 3_1:-26649696 None:intergenic
GGTTTGGCGATTTGGCTTCA+GGG 0.476471 3_1:+26650042 Msa0354660:CDS
CTCCCGCATACGCAGGGTCC+GGG 0.477983 3_1:-26650738 None:intergenic
CCAATTCACAAGGAAAGGTT+GGG 0.479764 3_1:-26649695 None:intergenic
CTCTTGGTTGATTGTCACCC+CGG 0.481676 3_1:+26649658 Msa0354660:five_prime_UTR
CTGAAGCTCTGATTTGATCA+TGG 0.484440 3_1:-26649825 None:intergenic
TGGGAGTAACTATGCAAGCC+GGG 0.489139 3_1:-26649676 None:intergenic
AGTGTACAAATTGAGGAAAT+GGG 0.497614 3_1:+26650261 Msa0354660:three_prime_UTR
CGCATACGCAGGGTCCGGGA+AGG 0.499824 3_1:-26650734 None:intergenic
ACAAAACAGTGTACAAATTG+AGG 0.507538 3_1:+26650254 Msa0354660:three_prime_UTR
TACACTTTATCTCATCATAA+CGG 0.509931 3_1:-26647017 None:intergenic
ATTGACCAATTCACAAGGAA+AGG 0.520990 3_1:-26649700 None:intergenic
GCTGCGTACAATACACCAAA+TGG 0.522967 3_1:+26650704 Msa0354660:three_prime_UTR
TCTGTCGTGGTCTTAAATAA+TGG 0.523689 3_1:+26650393 Msa0354660:three_prime_UTR
CTCTATGCTTGATAAATATG+TGG 0.528398 3_1:+26650172 Msa0354660:CDS
AGAGTAGCAGAAGAGTATAA+CGG 0.529896 3_1:+26650020 Msa0354660:CDS
GACCGTAATTGAGGCCGCAT+CGG 0.532845 3_1:+26650477 Msa0354660:three_prime_UTR
AGAGAGATAAAGATGTTGCA+GGG 0.533062 3_1:-26650610 None:intergenic
AGTTTGAGGACCCAAATAAA+CGG 0.533514 3_1:-26650366 None:intergenic
AAGTAAATTAGCAGGGTGCA+GGG 0.535934 3_1:-26650304 None:intergenic
CTGCAACATGACCGTAATTG+AGG 0.539059 3_1:+26650468 Msa0354660:three_prime_UTR
TTGGGAGTAACTATGCAAGC+CGG 0.542326 3_1:-26649677 None:intergenic
TTGTGACCAGATCGTGGTCG+CGG 0.546492 3_1:-26650417 None:intergenic
TTATTTAAGACCACGACAGA+GGG 0.557465 3_1:-26650390 None:intergenic
TATAAAGAAAGATGAAGCCC+AGG 0.573042 3_1:+26649745 Msa0354660:CDS
GTTGATATTGCACAGAATTG+TGG 0.578941 3_1:-26650531 None:intergenic
GCTGAGTACTCTCTTCCTCA+AGG 0.579129 3_1:-26646930 None:intergenic
CCTCAAACTCCCCTCTGTCG+TGG 0.589112 3_1:+26650380 Msa0354660:three_prime_UTR
TACAAATTGAGGAAATGGGT+AGG 0.589720 3_1:+26650265 Msa0354660:three_prime_UTR
GGCCGCATCGGCTGTAATTG+TGG 0.592958 3_1:+26650489 Msa0354660:three_prime_UTR
GCGTACAATACACCAAATGG+TGG 0.601920 3_1:+26650707 Msa0354660:three_prime_UTR
CTAAAGCTCCCGCATACGCA+GGG 0.605962 3_1:-26650744 None:intergenic
TCAATTATATGCTCCAACCA+TGG 0.606866 3_1:+26649718 Msa0354660:exon
AGTGACTGAATTTGAAGCTG+TGG 0.611769 3_1:+26650148 Msa0354660:CDS
TGGTGGCAACACTACTGAAG+AGG 0.616263 3_1:-26649805 None:intergenic
TTAAAGTGAAGGCGAAGCAG+TGG 0.629246 3_1:-26646956 None:intergenic
ATTATTTAAGACCACGACAG+AGG 0.636695 3_1:-26650391 None:intergenic
AGTAAATTAGCAGGGTGCAG+GGG 0.639599 3_1:-26650303 None:intergenic
AACCACAATTACAGCCGATG+CGG 0.644871 3_1:-26650491 None:intergenic
AGCAGTCACGGTAACAGATG+CGG 0.646818 3_1:-26650570 None:intergenic
CGTACAATACACCAAATGGT+GGG 0.668975 3_1:+26650708 Msa0354660:three_prime_UTR
GGGAGTAACTATGCAAGCCG+GGG 0.674597 3_1:-26649675 None:intergenic
GAGCAATTGAAATGCAAGAG+TGG 0.703600 3_1:+26650080 Msa0354660:CDS
AAGCTCTGATTTGATCATGG+TGG 0.703617 3_1:-26649822 None:intergenic
ATATAATTGACCAATTCACA+AGG 0.707148 3_1:-26649705 None:intergenic
TATTTAAGACCACGACAGAG+GGG 0.739792 3_1:-26650389 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTTTCAAAATATTAT+CGG + chr3_1:26647339-26647358 Msa0354660:intron 10.0%
!!! ATTATAAATTTTACCTGTTT+TGG - chr3_1:26647045-26647064 None:intergenic 15.0%
!! AATTAAGCTAATAATTATCA+AGG - chr3_1:26647143-26647162 None:intergenic 15.0%
!!! TTATCTTTTGTTATAGAAAT+AGG + chr3_1:26647481-26647500 Msa0354660:intron 15.0%
!!! ATGTATAATGATTAAGTTTT+TGG + chr3_1:26647696-26647715 Msa0354660:intron 15.0%
!!! ATGTATAATGATTAAGTTTT+TGG + chr3_1:26647696-26647715 Msa0354660:intron 15.0%
!! TTCAAAAATTTAATAGTAAG+TGG + chr3_1:26648360-26648379 Msa0354660:intron 15.0%
!!! TAAAAGTATCAATAAAAATC+TGG - chr3_1:26648507-26648526 None:intergenic 15.0%
!!! TATTTTGTTATAATTTGCAA+TGG + chr3_1:26649942-26649961 Msa0354660:intron 15.0%
!! AATGACCCTAAATTTAATTA+TGG + chr3_1:26647163-26647182 Msa0354660:intron 20.0%
!! AAGCTCCATAATTAAATTTA+GGG - chr3_1:26647171-26647190 None:intergenic 20.0%
!!! ATTTCACTTTTTCATTGATA+TGG + chr3_1:26647214-26647233 Msa0354660:intron 20.0%
!! GTTTCTATGATAGATTTATA+TGG + chr3_1:26647731-26647750 Msa0354660:intron 20.0%
!! GTTTCTATGATAGATTTATA+TGG + chr3_1:26647731-26647750 Msa0354660:intron 20.0%
!! ATACAAATTGTAAGATGTAT+AGG + chr3_1:26647878-26647897 Msa0354660:intron 20.0%
!! TACAAATTGTAAGATGTATA+GGG + chr3_1:26647879-26647898 Msa0354660:intron 20.0%
!!! CTAAGCAAAAATATTTTAAC+AGG - chr3_1:26648761-26648780 None:intergenic 20.0%
!!! GTAGTTTTTTCAACTATTAT+CGG + chr3_1:26649177-26649196 Msa0354660:intron 20.0%
!! AGAAACAACTTATATGTTTA+TGG - chr3_1:26649339-26649358 None:intergenic 20.0%
!! CTTAAATTTAGATGACTAAA+CGG + chr3_1:26649379-26649398 Msa0354660:intron 20.0%
!! TGAGAAGATTATATTAAGTT+AGG + chr3_1:26649561-26649580 Msa0354660:intron 20.0%
!! GAGAAGATTATATTAAGTTA+GGG + chr3_1:26649562-26649581 Msa0354660:intron 20.0%
!!! ATTTGAAATGTACAATTGTT+TGG + chr3_1:26649893-26649912 Msa0354660:intron 20.0%
!! GAAAAATAAGTAAATTAGCA+GGG - chr3_1:26650314-26650333 None:intergenic 20.0%
!! AGAAAAATAAGTAAATTAGC+AGG - chr3_1:26650315-26650334 None:intergenic 20.0%
! TACACTTTATCTCATCATAA+CGG - chr3_1:26647020-26647039 None:intergenic 25.0%
! CAAGCTCCATAATTAAATTT+AGG - chr3_1:26647172-26647191 None:intergenic 25.0%
! CTTATGTTATAGAAATTCAC+CGG + chr3_1:26647540-26647559 Msa0354660:intron 25.0%
!!! TTTTTTTTTGAGATCAGTTG+TGG + chr3_1:26647636-26647655 Msa0354660:intron 25.0%
! TGATAGATTTATATGGTGTT+AGG + chr3_1:26647738-26647757 Msa0354660:intron 25.0%
!! GATAGATTTATATGGTGTTA+GGG + chr3_1:26647739-26647758 Msa0354660:intron 25.0%
! TGATAGATTTATATGGTGTT+AGG + chr3_1:26647738-26647757 Msa0354660:intron 25.0%
!! GATAGATTTATATGGTGTTA+GGG + chr3_1:26647739-26647758 Msa0354660:intron 25.0%
! ATTTGTATTCAGATCTGAAA+AGG - chr3_1:26647866-26647885 None:intergenic 25.0%
! ACAAATTGTAAGATGTATAG+GGG + chr3_1:26647880-26647899 Msa0354660:intron 25.0%
!! AAAGATATAGCAAGTTGTTT+TGG - chr3_1:26648049-26648068 None:intergenic 25.0%
!! ACAGATGATTGATTCTAATA+CGG - chr3_1:26648121-26648140 None:intergenic 25.0%
! AAATCTGCTTATTGTTTGTT+TGG - chr3_1:26648429-26648448 None:intergenic 25.0%
!! ATAAAAATCTGGTTTGCAAA+TGG - chr3_1:26648496-26648515 None:intergenic 25.0%
!!! GATACTTTTAGTATTTGTTG+AGG + chr3_1:26648517-26648536 Msa0354660:intron 25.0%
!!! TTTGTATTGGTTCTTGAATT+GGG + chr3_1:26648578-26648597 Msa0354660:intron 25.0%
!! ATTAAACAGTCTAAAATACC+GGG - chr3_1:26648726-26648745 None:intergenic 25.0%
!! CATTAAACAGTCTAAAATAC+CGG - chr3_1:26648727-26648746 None:intergenic 25.0%
!!! TATTTTAACAGGCAATTATC+AGG - chr3_1:26648750-26648769 None:intergenic 25.0%
! TCTGAAAATCTATATGCTAA+TGG + chr3_1:26648955-26648974 Msa0354660:intron 25.0%
! TAAACATCACATATTACTAG+TGG + chr3_1:26649061-26649080 Msa0354660:intron 25.0%
! TTGTAATGTTGTAAAAGACT+TGG + chr3_1:26649245-26649264 Msa0354660:intron 25.0%
! TGTAATGTTGTAAAAGACTT+GGG + chr3_1:26649246-26649265 Msa0354660:intron 25.0%
! ACAACTTATATGTTTATGGA+CGG - chr3_1:26649335-26649354 None:intergenic 25.0%
! TAATACCAATCTCCAAAATA+AGG + chr3_1:26649518-26649537 Msa0354660:intron 25.0%
! TGTGAGCTTGTAATTATAAA+AGG + chr3_1:26649591-26649610 Msa0354660:intron 25.0%
! GTGAGCTTGTAATTATAAAA+GGG + chr3_1:26649592-26649611 Msa0354660:intron 25.0%
! ATATAATTGACCAATTCACA+AGG - chr3_1:26649708-26649727 None:intergenic 25.0%
! ATCTTTCTTTATATTAGCCA+TGG - chr3_1:26649738-26649757 None:intergenic 25.0%
!! TAATAATCATCTAGAGCTAT+TGG + chr3_1:26649983-26650002 Msa0354660:CDS 25.0%
!! TCTCTCAATTTGTATCATTT+TGG + chr3_1:26650332-26650351 Msa0354660:three_prime_UTR 25.0%
!!! AAAGTTTTAAATAGCAGTCA+CGG - chr3_1:26650585-26650604 None:intergenic 25.0%
GATAAAGTGTAATCCAAAAC+AGG + chr3_1:26647029-26647048 Msa0354660:five_prime_UTR 30.0%
AGAACACGAGATAAGAAAAA+AGG - chr3_1:26647088-26647107 None:intergenic 30.0%
TTATCAAGGAAGTTGAAAAC+AGG - chr3_1:26647129-26647148 None:intergenic 30.0%
CCTTTAATCAAACAACACAT+AGG - chr3_1:26647246-26647265 None:intergenic 30.0%
!! CCTATGTGTTGTTTGATTAA+AGG + chr3_1:26647243-26647262 Msa0354660:intron 30.0%
! TGTTTGATTAAAGGCATGTT+TGG + chr3_1:26647252-26647271 Msa0354660:intron 30.0%
ATGTCAATCACTACACATAT+CGG - chr3_1:26647281-26647300 None:intergenic 30.0%
TTCAAAGTAATCACATGTGT+TGG - chr3_1:26647314-26647333 None:intergenic 30.0%
ACAATCGTCATTATTAAGAC+CGG - chr3_1:26647562-26647581 None:intergenic 30.0%
! ATTTATATGGTGTTAGGGTT+AGG + chr3_1:26647744-26647763 Msa0354660:intron 30.0%
! ATTTATATGGTGTTAGGGTT+AGG + chr3_1:26647744-26647763 Msa0354660:intron 30.0%
CAAATTGTAAGATGTATAGG+GGG + chr3_1:26647881-26647900 Msa0354660:intron 30.0%
AAATTGTAAGATGTATAGGG+GGG + chr3_1:26647882-26647901 Msa0354660:intron 30.0%
GTATTAGAATCAATCATCTG+TGG + chr3_1:26648120-26648139 Msa0354660:intron 30.0%
!! TTCCTCTTATTTCTGTTCAT+TGG + chr3_1:26648313-26648332 Msa0354660:intron 30.0%
TACCAATGAACAGAAATAAG+AGG - chr3_1:26648318-26648337 None:intergenic 30.0%
! GTTTTTCTTCAAGAAATGGA+TGG - chr3_1:26648402-26648421 None:intergenic 30.0%
! CACAGTTTTTCTTCAAGAAA+TGG - chr3_1:26648406-26648425 None:intergenic 30.0%
! AACCAATGATGAGATTGTTT+TGG + chr3_1:26648549-26648568 Msa0354660:intron 30.0%
ATCCAAAACAATCTCATCAT+TGG - chr3_1:26648554-26648573 None:intergenic 30.0%
!! GTTTTGGATTTGCTTTGTAT+TGG + chr3_1:26648565-26648584 Msa0354660:intron 30.0%
!! CTTTGTATTGGTTCTTGAAT+TGG + chr3_1:26648577-26648596 Msa0354660:intron 30.0%
GTATTATACACAATGATGAG+AGG + chr3_1:26648637-26648656 Msa0354660:intron 30.0%
! GGTCATTCTGCATATTATTT+TGG + chr3_1:26648658-26648677 Msa0354660:intron 30.0%
! TGTAATTGTGATAGTGACAT+TGG - chr3_1:26649017-26649036 None:intergenic 30.0%
GTAATGTTGTAAAAGACTTG+GGG + chr3_1:26649247-26649266 Msa0354660:intron 30.0%
TAATGTTGTAAAAGACTTGG+GGG + chr3_1:26649248-26649267 Msa0354660:intron 30.0%
AATTTAGATGACTAAACGGA+AGG + chr3_1:26649383-26649402 Msa0354660:intron 30.0%
!!! CTTATTTTGGAGATTGGTAT+TGG - chr3_1:26649441-26649460 None:intergenic 30.0%
CAATACCAATCTCCAAAATA+AGG + chr3_1:26649439-26649458 Msa0354660:intron 30.0%
!!! AATCTCCTTATTTTGGAGAT+TGG - chr3_1:26649447-26649466 None:intergenic 30.0%
!! ATGTGTGAATCTCCTTATTT+TGG - chr3_1:26649454-26649473 None:intergenic 30.0%
TGAAATTTACCGCATAATGT+TGG + chr3_1:26649491-26649510 Msa0354660:intron 30.0%
TATTAGAGACCAACATTATG+CGG - chr3_1:26649503-26649522 None:intergenic 30.0%
!!! AATCTCCTTATTTTGGAGAT+TGG - chr3_1:26649447-26649466 None:intergenic 30.0%
!! ATGTGTGAATCTCCTTATTT+TGG - chr3_1:26649454-26649473 None:intergenic 30.0%
!! ATCAACCAAGAGTTGTTTTA+TGG - chr3_1:26649650-26649669 None:intergenic 30.0%
TTCTTTATATTAGCCATGGT+TGG - chr3_1:26649734-26649753 None:intergenic 30.0%
CTCTATGCTTGATAAATATG+TGG + chr3_1:26650172-26650191 Msa0354660:CDS 30.0%
!! TGATAAATATGTGGCTTTGT+TGG + chr3_1:26650181-26650200 Msa0354660:CDS 30.0%
!! TAATCAGGAAGATGGATTTT+TGG - chr3_1:26650234-26650253 None:intergenic 30.0%
!! TGTTTTGTTAATCAGGAAGA+TGG - chr3_1:26650242-26650261 None:intergenic 30.0%
!! TGTACACTGTTTTGTTAATC+AGG - chr3_1:26650249-26650268 None:intergenic 30.0%
ACAAAACAGTGTACAAATTG+AGG + chr3_1:26650254-26650273 Msa0354660:three_prime_UTR 30.0%
AGTGTACAAATTGAGGAAAT+GGG + chr3_1:26650261-26650280 Msa0354660:three_prime_UTR 30.0%
!! CAATTTGTATCATTTTGGTC+CGG + chr3_1:26650337-26650356 Msa0354660:three_prime_UTR 30.0%
GTAAACATAAGGAGATGAAA+AGG - chr3_1:26650640-26650659 None:intergenic 30.0%
AGGAAAATGGAGTAAACATA+AGG - chr3_1:26650651-26650670 None:intergenic 30.0%
! TTATGTTTACTCCATTTTCC+TGG + chr3_1:26650650-26650669 Msa0354660:three_prime_UTR 30.0%
!!! AGCAGCAGATTTTAAAGTGA+AGG - chr3_1:26646970-26646989 None:intergenic 35.0%
! ATGACGATTGTTATTGAAGC+TGG + chr3_1:26647571-26647590 Msa0354660:intron 35.0%
GTTAACAATGTTGGACATCT+CGG - chr3_1:26647597-26647616 None:intergenic 35.0%
TCCAACATTGTTAACGAGAT+AGG + chr3_1:26647602-26647621 Msa0354660:intron 35.0%
ACCTATCTCGTTAACAATGT+TGG - chr3_1:26647606-26647625 None:intergenic 35.0%
! GTGGAAATATTTGTTTGCTG+AGG + chr3_1:26647655-26647674 Msa0354660:intron 35.0%
!!! TTGTCCTAGTTCCAGTTTTT+TGG + chr3_1:26648018-26648037 Msa0354660:intron 35.0%
TTTAATAGTAAGTGGCAGAG+TGG + chr3_1:26648368-26648387 Msa0354660:intron 35.0%
AGTGAGAAACATATCCTTGA+TGG + chr3_1:26648465-26648484 Msa0354660:intron 35.0%
GTGAGAAACATATCCTTGAT+GGG + chr3_1:26648466-26648485 Msa0354660:intron 35.0%
!! ATAGTGGGGAGATTTTTGAA+AGG + chr3_1:26648615-26648634 Msa0354660:intron 35.0%
! ATTTTTGCTTAGAACCCACT+TGG + chr3_1:26648769-26648788 Msa0354660:intron 35.0%
! TTTTTGCTTAGAACCCACTT+GGG + chr3_1:26648770-26648789 Msa0354660:intron 35.0%
GTCACTGTTTATGTAGCAAT+CGG + chr3_1:26649083-26649102 Msa0354660:intron 35.0%
TGTTTGTGTCAGTGTTTCAT+AGG + chr3_1:26649220-26649239 Msa0354660:intron 35.0%
TTATATGTTTATGGACGGAG+GGG - chr3_1:26649330-26649349 None:intergenic 35.0%
CTTATATGTTTATGGACGGA+GGG - chr3_1:26649331-26649350 None:intergenic 35.0%
ACTTATATGTTTATGGACGG+AGG - chr3_1:26649332-26649351 None:intergenic 35.0%
TGGAATTTACCGCATAATGT+TGG + chr3_1:26649412-26649431 Msa0354660:intron 35.0%
TATTGGAGACCAACATTATG+CGG - chr3_1:26649424-26649443 None:intergenic 35.0%
AAGGAGATTCACACATCTAA+CGG + chr3_1:26649458-26649477 Msa0354660:intron 35.0%
!! TTTTTCAAACAACTGCAGCT+TGG + chr3_1:26649623-26649642 Msa0354660:intron 35.0%
TTGGACCATAAAACAACTCT+TGG + chr3_1:26649642-26649661 Msa0354660:five_prime_UTR 35.0%
ATTGACCAATTCACAAGGAA+AGG - chr3_1:26649703-26649722 None:intergenic 35.0%
TCAATTATATGCTCCAACCA+TGG + chr3_1:26649718-26649737 Msa0354660:exon 35.0%
TATAAAGAAAGATGAAGCCC+AGG + chr3_1:26649745-26649764 Msa0354660:CDS 35.0%
TAGAGGGTTAGAGAAAGAAT+GGG - chr3_1:26649860-26649879 None:intergenic 35.0%
! AGAGTAGCAGAAGAGTATAA+CGG + chr3_1:26650020-26650039 Msa0354660:CDS 35.0%
!!! TTTTGGTTTGGTACTGAGAT+TGG - chr3_1:26650217-26650236 None:intergenic 35.0%
!!! AGGAAGATGGATTTTTGGTT+TGG - chr3_1:26650229-26650248 None:intergenic 35.0%
CAGTGTACAAATTGAGGAAA+TGG + chr3_1:26650260-26650279 Msa0354660:three_prime_UTR 35.0%
TACAAATTGAGGAAATGGGT+AGG + chr3_1:26650265-26650284 Msa0354660:three_prime_UTR 35.0%
TCCGGTTTATACCGTTTATT+TGG + chr3_1:26650355-26650374 Msa0354660:three_prime_UTR 35.0%
CCCAAATAAACGGTATAAAC+CGG - chr3_1:26650359-26650378 None:intergenic 35.0%
CCGGTTTATACCGTTTATTT+GGG + chr3_1:26650356-26650375 Msa0354660:three_prime_UTR 35.0%
AGTTTGAGGACCCAAATAAA+CGG - chr3_1:26650369-26650388 None:intergenic 35.0%
! TTATTTAAGACCACGACAGA+GGG - chr3_1:26650393-26650412 None:intergenic 35.0%
! ATTATTTAAGACCACGACAG+AGG - chr3_1:26650394-26650413 None:intergenic 35.0%
! TCTGTCGTGGTCTTAAATAA+TGG + chr3_1:26650393-26650412 Msa0354660:three_prime_UTR 35.0%
GTTGATATTGCACAGAATTG+TGG - chr3_1:26650534-26650553 None:intergenic 35.0%
AGAGAGATAAAGATGTTGCA+GGG - chr3_1:26650613-26650632 None:intergenic 35.0%
CATTGTTAACGAGATAGGTG+TGG + chr3_1:26647607-26647626 Msa0354660:intron 40.0%
!!! CTAGTTCCAGTTTTTTGGCT+GGG + chr3_1:26648023-26648042 Msa0354660:intron 40.0%
AGTTATCATGCTTTAGCACC+CGG + chr3_1:26648705-26648724 Msa0354660:intron 40.0%
! ATTCTCCCTGGTGCTAATTT+CGG + chr3_1:26648853-26648872 Msa0354660:intron 40.0%
AAACAAGCGTGCTATCTTGT+TGG + chr3_1:26648985-26649004 Msa0354660:intron 40.0%
CTTCTGATTGAAGATGTGTC+CGG + chr3_1:26649108-26649127 Msa0354660:intron 40.0%
GTTATGTGTTAGTGTCGTGT+TGG - chr3_1:26649143-26649162 None:intergenic 40.0%
GACTAAACGGAAGGAAAACA+TGG + chr3_1:26649392-26649411 Msa0354660:intron 40.0%
AGATTCACACATCTAACGGA+AGG + chr3_1:26649462-26649481 Msa0354660:intron 40.0%
CCAATTCACAAGGAAAGGTT+GGG - chr3_1:26649698-26649717 None:intergenic 40.0%
ACCAATTCACAAGGAAAGGT+TGG - chr3_1:26649699-26649718 None:intergenic 40.0%
! AAGCTCTGATTTGATCATGG+TGG - chr3_1:26649825-26649844 None:intergenic 40.0%
! CTGAAGCTCTGATTTGATCA+TGG - chr3_1:26649828-26649847 None:intergenic 40.0%
CATGATCAAATCAGAGCTTC+AGG + chr3_1:26649826-26649845 Msa0354660:CDS 40.0%
AGAGGGTTAGAGAAAGAATG+GGG - chr3_1:26649859-26649878 None:intergenic 40.0%
GTAGAGGGTTAGAGAAAGAA+TGG - chr3_1:26649861-26649880 None:intergenic 40.0%
AATCGTCAAGTGTCTGTAGA+GGG - chr3_1:26649876-26649895 None:intergenic 40.0%
AAATCGTCAAGTGTCTGTAG+AGG - chr3_1:26649877-26649896 None:intergenic 40.0%
! GAGTAGCAGAAGAGTATAAC+GGG + chr3_1:26650021-26650040 Msa0354660:CDS 40.0%
GAGCAATTGAAATGCAAGAG+TGG + chr3_1:26650080-26650099 Msa0354660:CDS 40.0%
TTCAGTCACTTGCTTCTCAA+TGG - chr3_1:26650138-26650157 None:intergenic 40.0%
! AGTGACTGAATTTGAAGCTG+TGG + chr3_1:26650148-26650167 Msa0354660:CDS 40.0%
AAGTAAATTAGCAGGGTGCA+GGG - chr3_1:26650307-26650326 None:intergenic 40.0%
TAAGTAAATTAGCAGGGTGC+AGG - chr3_1:26650308-26650327 None:intergenic 40.0%
! TATTTAAGACCACGACAGAG+GGG - chr3_1:26650392-26650411 None:intergenic 40.0%
!! ATTTTGTTGTGACCAGATCG+TGG - chr3_1:26650426-26650445 None:intergenic 40.0%
ACGATCTGGTCACAACAAAA+TGG + chr3_1:26650425-26650444 Msa0354660:three_prime_UTR 40.0%
GAGAGAGATAAAGATGTTGC+AGG - chr3_1:26650614-26650633 None:intergenic 40.0%
AGCCTCTTGTGTAAAAAGCA+GGG + chr3_1:26650677-26650696 Msa0354660:three_prime_UTR 40.0%
!!! TACCCTGCTTTTTACACAAG+AGG - chr3_1:26650682-26650701 None:intergenic 40.0%
CTTGTGTAAAAAGCAGGGTA+AGG + chr3_1:26650682-26650701 Msa0354660:three_prime_UTR 40.0%
CGTACAATACACCAAATGGT+GGG + chr3_1:26650708-26650727 Msa0354660:three_prime_UTR 40.0%
TTAAAGTGAAGGCGAAGCAG+TGG - chr3_1:26646959-26646978 None:intergenic 45.0%
CATTGACACCAAACACGACA+CGG - chr3_1:26647382-26647401 None:intergenic 45.0%
TGTCAGTGCTTCATTGCTCT+TGG + chr3_1:26647406-26647425 Msa0354660:intron 45.0%
! TTTGTTTGCTGAGGCAGTAG+TGG + chr3_1:26647664-26647683 Msa0354660:intron 45.0%
CCAGCCAAAAAACTGGAACT+AGG - chr3_1:26648025-26648044 None:intergenic 45.0%
!!! CCTAGTTCCAGTTTTTTGGC+TGG + chr3_1:26648022-26648041 Msa0354660:intron 45.0%
! TTTTGGCCCAGCCAAAAAAC+TGG - chr3_1:26648032-26648051 None:intergenic 45.0%
TGCAAATGGAGAACCCATCA+AGG - chr3_1:26648482-26648501 None:intergenic 45.0%
! GTAGTGCTTGCTAGCATAGT+GGG + chr3_1:26648600-26648619 Msa0354660:intron 45.0%
! TAGTGCTTGCTAGCATAGTG+GGG + chr3_1:26648601-26648620 Msa0354660:intron 45.0%
!! TTGGCCTAGTGTTGTTTGCT+TGG + chr3_1:26648793-26648812 Msa0354660:intron 45.0%
!! TGGCCTAGTGTTGTTTGCTT+GGG + chr3_1:26648794-26648813 Msa0354660:intron 45.0%
! CTTGAGAAGAGCACACTCTA+TGG - chr3_1:26648821-26648840 None:intergenic 45.0%
CATAGAGTGTGCTCTTCTCA+AGG + chr3_1:26648819-26648838 Msa0354660:intron 45.0%
TGTGCTCTTCTCAAGGTTTC+AGG + chr3_1:26648826-26648845 Msa0354660:intron 45.0%
TGTTGTAAAAGACTTGGGGG+AGG + chr3_1:26649251-26649270 Msa0354660:intron 45.0%
TTGGGAGTAACTATGCAAGC+CGG - chr3_1:26649680-26649699 None:intergenic 45.0%
CCCAACCTTTCCTTGTGAAT+TGG + chr3_1:26649695-26649714 Msa0354660:five_prime_UTR 45.0%
TTCATCACGCTTCTGATCCT+GGG - chr3_1:26649765-26649784 None:intergenic 45.0%
TTGCAATGGTTGTGTGTGTC+AGG + chr3_1:26649956-26649975 Msa0354660:intron 45.0%
TGCAATGGTTGTGTGTGTCA+GGG + chr3_1:26649957-26649976 Msa0354660:intron 45.0%
!! GCAGAAGAGTATAACGGGTT+TGG + chr3_1:26650026-26650045 Msa0354660:CDS 45.0%
!! GTATAACGGGTTTGGCGATT+TGG + chr3_1:26650034-26650053 Msa0354660:CDS 45.0%
! CGATTTGGCTTCAGGGTTAA+GGG + chr3_1:26650049-26650068 Msa0354660:CDS 45.0%
!! TTCAGGGTTAAGGGTGTTTG+TGG + chr3_1:26650058-26650077 Msa0354660:CDS 45.0%
AGTAAATTAGCAGGGTGCAG+GGG - chr3_1:26650306-26650325 None:intergenic 45.0%
CTGCAACATGACCGTAATTG+AGG + chr3_1:26650468-26650487 Msa0354660:three_prime_UTR 45.0%
AACCACAATTACAGCCGATG+CGG - chr3_1:26650494-26650513 None:intergenic 45.0%
! AGAGGCTGTTTCCAGGAAAA+TGG - chr3_1:26650664-26650683 None:intergenic 45.0%
! TTACACAAGAGGCTGTTTCC+AGG - chr3_1:26650671-26650690 None:intergenic 45.0%
CAGCCTCTTGTGTAAAAAGC+AGG + chr3_1:26650676-26650695 Msa0354660:three_prime_UTR 45.0%
GCTGCGTACAATACACCAAA+TGG + chr3_1:26650704-26650723 Msa0354660:three_prime_UTR 45.0%
GCGTACAATACACCAAATGG+TGG + chr3_1:26650707-26650726 Msa0354660:three_prime_UTR 45.0%
! GGTAGTGCTTGCTAGCATAG+TGG + chr3_1:26648599-26648618 Msa0354660:intron 50.0%
TGCTTAGAACCCACTTGGGT+TGG + chr3_1:26648774-26648793 Msa0354660:intron 50.0%
AACACTAGGCCAACCCAAGT+GGG - chr3_1:26648786-26648805 None:intergenic 50.0%
GGTCCCAAGCAAACAACACT+AGG - chr3_1:26648800-26648819 None:intergenic 50.0%
!! GTTTCAGGTTCGATTCTCCC+TGG + chr3_1:26648841-26648860 Msa0354660:intron 50.0%
! TCCCTGGTGCTAATTTCGGT+GGG + chr3_1:26648857-26648876 Msa0354660:intron 50.0%
ACCCACCGAAATTAGCACCA+GGG - chr3_1:26648861-26648880 None:intergenic 50.0%
AACCCACCGAAATTAGCACC+AGG - chr3_1:26648862-26648881 None:intergenic 50.0%
! TGTTAGTGTCGTGTTGGCGT+CGG - chr3_1:26649137-26649156 None:intergenic 50.0%
TGTAAAAGACTTGGGGGAGG+AGG + chr3_1:26649254-26649273 Msa0354660:intron 50.0%
GTAAAAGACTTGGGGGAGGA+GGG + chr3_1:26649255-26649274 Msa0354660:intron 50.0%
TAAAAGACTTGGGGGAGGAG+GGG + chr3_1:26649256-26649275 Msa0354660:intron 50.0%
TATGTTTATGGACGGAGGGG+AGG - chr3_1:26649327-26649346 None:intergenic 50.0%
CTCTTGGTTGATTGTCACCC+CGG + chr3_1:26649658-26649677 Msa0354660:five_prime_UTR 50.0%
TGGGAGTAACTATGCAAGCC+GGG - chr3_1:26649679-26649698 None:intergenic 50.0%
GTTCATCACGCTTCTGATCC+TGG - chr3_1:26649766-26649785 None:intergenic 50.0%
TGGTGGCAACACTACTGAAG+AGG - chr3_1:26649808-26649827 None:intergenic 50.0%
GCAATGGTTGTGTGTGTCAG+GGG + chr3_1:26649958-26649977 Msa0354660:intron 50.0%
! GGTTTGGCGATTTGGCTTCA+GGG + chr3_1:26650042-26650061 Msa0354660:CDS 50.0%
! GCGATTTGGCTTCAGGGTTA+AGG + chr3_1:26650048-26650067 Msa0354660:CDS 50.0%
AGCAGTCACGGTAACAGATG+CGG - chr3_1:26650573-26650592 None:intergenic 50.0%
GTGAGTGTCCGTGTCGTGTT+TGG + chr3_1:26647371-26647390 Msa0354660:intron 55.0%
CAACACTAGGCCAACCCAAG+TGG - chr3_1:26648787-26648806 None:intergenic 55.0%
! CTCCCTGGTGCTAATTTCGG+TGG + chr3_1:26648856-26648875 Msa0354660:intron 55.0%
GGGAGTAACTATGCAAGCCG+GGG - chr3_1:26649678-26649697 None:intergenic 55.0%
! GGGTTTGGCGATTTGGCTTC+AGG + chr3_1:26650041-26650060 Msa0354660:CDS 55.0%
TTGTGACCAGATCGTGGTCG+CGG - chr3_1:26650420-26650439 None:intergenic 55.0%
GACCGTAATTGAGGCCGCAT+CGG + chr3_1:26650477-26650496 Msa0354660:three_prime_UTR 55.0%
AGCCGATGCGGCCTCAATTA+CGG - chr3_1:26650482-26650501 None:intergenic 55.0%
CCACGACAGAGGGGAGTTTG+AGG - chr3_1:26650383-26650402 None:intergenic 60.0%
CCTCAAACTCCCCTCTGTCG+TGG + chr3_1:26650380-26650399 Msa0354660:three_prime_UTR 60.0%
AATGGTCCGCGACCACGATC+TGG + chr3_1:26650411-26650430 Msa0354660:three_prime_UTR 60.0%
!! GGCCGCATCGGCTGTAATTG+TGG + chr3_1:26650489-26650508 Msa0354660:three_prime_UTR 60.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr3_1:26650720-26650739 Msa0354660:three_prime_UTR 60.0%
! GTCGTGTTGGCGTCGGACAC+CGG - chr3_1:26649130-26649149 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG - chr3_1:26650722-26650741 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 26646932 26650751 26646932 ID=Msa0354660;Name=Msa0354660
chr3_1 mRNA 26646932 26650751 26646932 ID=Msa0354660-mRNA-1;Parent=Msa0354660;Name=Msa0354660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=215|1|1|1|0.5|0.33|3|501|127
chr3_1 exon 26646932 26647050 26646932 ID=Msa0354660-mRNA-1:exon:5800;Parent=Msa0354660-mRNA-1
chr3_1 exon 26649641 26649847 26649641 ID=Msa0354660-mRNA-1:exon:5801;Parent=Msa0354660-mRNA-1
chr3_1 exon 26649978 26650751 26649978 ID=Msa0354660-mRNA-1:exon:5802;Parent=Msa0354660-mRNA-1
chr3_1 five_prime_UTR 26646932 26647050 26646932 ID=Msa0354660-mRNA-1:five_prime_utr;Parent=Msa0354660-mRNA-1
chr3_1 five_prime_UTR 26649641 26649736 26649641 ID=Msa0354660-mRNA-1:five_prime_utr;Parent=Msa0354660-mRNA-1
chr3_1 CDS 26649737 26649847 26649737 ID=Msa0354660-mRNA-1:cds;Parent=Msa0354660-mRNA-1
chr3_1 CDS 26649978 26650250 26649978 ID=Msa0354660-mRNA-1:cds;Parent=Msa0354660-mRNA-1
chr3_1 three_prime_UTR 26650251 26650751 26650251 ID=Msa0354660-mRNA-1:three_prime_utr;Parent=Msa0354660-mRNA-1
Gene Sequence

>Msa0354660

ATGGCTAATATAAAGAAAGATGAAGCCCAGGATCAGAAGCGTGATGAACTTGCTGAATCACTTAATGACCTCTTCAGTAGTGTTGCCACCATGATCAAATCAGAGCTTCAGGGGACTAATAATCATCTAGAGCTATTGGAGAAAATGAATCTGAGAGTAGCAGAAGAGTATAACGGGTTTGGCGATTTGGCTTCAGGGTTAAGGGTGTTTGTGGAGCAATTGAAATGCAAGAGTGGTAGCTTTAATGAATATGTTGAGCAGATTGATGCCATTGAGAAGCAAGTGACTGAATTTGAAGCTGTGGTCTCTATGCTTGATAAATATGTGGCTTTGTTGGAATCTAGAGTCCAATCTCAGTACCAAACCAAAAATCCATCTTCCTGA
Protein sequence

>Msa0354660

MANIKKDEAQDQKRDELAESLNDLFSSVATMIKSELQGTNNHLELLEKMNLRVAEEYNGFGDLASGLRVFVEQLKCKSGSFNEYVEQIDAIEKQVTEFEAVVSMLDKYVALLESRVQSQYQTKNPSS*