AlfalfaGEDB | Msa0101020

M. sativa cultivar zhongmu-4 / Msa0101020

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0008150 GO:0032886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007
PFAM: PF13414.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101020	sp\|F4IRM4\|PHOX1_ARATH	59.164	742	264	15	1	707	1	738	0.0	832

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101020	A0A072VDJ0	97.179	709	19	1	1	709	1	708	0.0	1429

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002680	Msa0101020	0.809418	1.911228e-50	-8.615850e-47
Msa0007990	Msa0101020	0.806097	9.703243e-50	-8.615850e-47
Msa0008220	Msa0101020	0.825629	4.222637e-54	-8.615850e-47
Msa0010770	Msa0101020	0.847601	1.043266e-59	-8.615850e-47
Msa0012710	Msa0101020	0.823507	1.334663e-53	-8.615850e-47
Msa0014570	Msa0101020	0.806806	6.879841e-50	-8.615850e-47
Msa0015670	Msa0101020	0.807224	5.611841e-50	-8.615850e-47
Msa0017320	Msa0101020	0.844192	8.801564e-59	-8.615850e-47
Msa0021210	Msa0101020	0.800662	1.297393e-48	-8.615850e-47
Msa0022550	Msa0101020	0.825309	5.028394e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101020	MtrunA17_Chr1g0149561	97.179	709	19	1	1	709	1	708	0.0	1429
Msa0101020	MtrunA17_Chr4g0075481	36.207	754	371	14	1	659	1	739	2.62e-144	440
Msa0101020	MtrunA17_Chr2g0289351	37.642	441	196	10	251	662	50	440	5.53e-89	286
Msa0101020	MtrunA17_Chr2g0304261	57.377	183	73	3	6	185	2	182	1.42e-60	216
Msa0101020	MtrunA17_Chr7g0237741	31.646	158	93	5	7	152	52	206	4.42e-16	79.0
Msa0101020	MtrunA17_Chr2g0323971	33.913	115	70	4	41	152	98	209	5.91e-14	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101020	AT2G25290.2	59.164	742	264	15	1	707	1	738	0.0	832
Msa0101020	AT2G25290.3	59.164	742	264	15	1	707	1	738	0.0	832
Msa0101020	AT2G25290.4	59.164	742	264	15	1	707	1	738	0.0	832
Msa0101020	AT2G25290.1	59.164	742	264	15	1	707	1	738	0.0	832
Msa0101020	AT4G32070.2	55.357	784	264	18	1	707	1	775	0.0	808
Msa0101020	AT4G32070.1	55.357	784	264	18	1	707	1	775	0.0	808
Msa0101020	AT5G20360.5	39.623	689	385	13	38	705	33	711	1.29e-163	488
Msa0101020	AT5G20360.6	39.623	689	385	13	38	705	33	711	1.29e-163	488
Msa0101020	AT5G20360.7	39.623	689	385	13	38	705	63	741	2.63e-163	489
Msa0101020	AT5G20360.4	39.623	689	385	13	38	705	63	741	2.63e-163	489

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Find 217 sgRNAs with CRISPR-Local

Find 357 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGATGTTGATGTGGG+AGG	0.621444	1_3:+7521628	Msa0101020:CDS
AAAATATTGAAGAATAAAAT+AGG	0.304235	1_3:-7522250	None:intergenic
AAAATTGTTCCAGGATGTAA+TGG	0.443210	1_3:-7522185	None:intergenic
AAATGTTAATGTTAATGTTA+AGG	0.428883	1_3:+7520659	Msa0101020:CDS
AACAATAAGAATATCGAAGT+TGG	0.461398	1_3:+7521554	Msa0101020:CDS
AACAGAACGCTCATACAATA+AGG	0.415328	1_3:-7521810	None:intergenic
AACATGGGAGGAATGTTTGG+AGG	0.573058	1_3:+7521856	Msa0101020:CDS
AACATTAACATTTCCAACAT+TGG	0.459292	1_3:-7520649	None:intergenic
AACTTCATACTACAATTCAC+CGG	0.617885	1_3:-7520813	None:intergenic
AAGAAAGGGCATAAAGGAGA+AGG	0.575305	1_3:+7520582	Msa0101020:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAACAACAACAAGAACAA+AGG	+	chr1_3:7520024-7520043	Msa0101020:CDS	30.0%
TCACAAGAATTAAGAGAAGA+AGG	+	chr1_3:7520099-7520118	Msa0101020:CDS	30.0%
TTCCAAAAAAAAGACAACGA+AGG	+	chr1_3:7520135-7520154	Msa0101020:CDS	30.0%
TGATAGTTTGAGGATTACTA+TGG	+	chr1_3:7520446-7520465	Msa0101020:CDS	30.0%
ATTAACATTTCCAACATTGG+TGG	-	chr1_3:7520649-7520668	None:intergenic	30.0%
AGTTGTTAAGGAAGATAAAG+CGG	+	chr1_3:7520737-7520756	Msa0101020:CDS	30.0%
TATATGGAGAGGATATTAGA+TGG	+	chr1_3:7520781-7520800	Msa0101020:CDS	30.0%
ATATGGAGAGGATATTAGAT+GGG	+	chr1_3:7520782-7520801	Msa0101020:CDS	30.0%
AACTTCATACTACAATTCAC+CGG	-	chr1_3:7520816-7520835	None:intergenic	30.0%
TTCTTGTGAAGTATAAGGAT+AGG	+	chr1_3:7520880-7520899	Msa0101020:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	7519727	7524131	7519727	ID=Msa0101020;Name=Msa0101020
chr1_3	mRNA	7519727	7524131	7519727	ID=Msa0101020-mRNA-1;Parent=Msa0101020;Name=Msa0101020-mRNA-1;_AED=0.01;_eAED=0.01;_QI=252\|1\|1\|1\|0.5\|0.33\|3\|443\|709
chr1_3	exon	7519727	7521971	7519727	ID=Msa0101020-mRNA-1:exon:2954;Parent=Msa0101020-mRNA-1
chr1_3	exon	7522073	7522300	7522073	ID=Msa0101020-mRNA-1:exon:2955;Parent=Msa0101020-mRNA-1
chr1_3	exon	7523780	7524131	7523780	ID=Msa0101020-mRNA-1:exon:2956;Parent=Msa0101020-mRNA-1
chr1_3	five_prime_UTR	7519727	7519978	7519727	ID=Msa0101020-mRNA-1:five_prime_utr;Parent=Msa0101020-mRNA-1
chr1_3	CDS	7519979	7521971	7519979	ID=Msa0101020-mRNA-1:cds;Parent=Msa0101020-mRNA-1
chr1_3	CDS	7522073	7522209	7522073	ID=Msa0101020-mRNA-1:cds;Parent=Msa0101020-mRNA-1
chr1_3	three_prime_UTR	7522210	7522300	7522210	ID=Msa0101020-mRNA-1:three_prime_utr;Parent=Msa0101020-mRNA-1
chr1_3	three_prime_UTR	7523780	7524131	7523780	ID=Msa0101020-mRNA-1:three_prime_utr;Parent=Msa0101020-mRNA-1

Gene Sequence

>Msa0101020

ATGGGGAAACCAGCTGGAAAAAAGAAGGAACATGAATCACCAAAATCAAACAACAACAAGAACAAAGGTTACGAACGTTCAACATCATTCGACGATGACACAGCCATGTTCATCAACATGTCACAAGAATTAAGAGAAGAAGGTAACAAACTTTTCTTCCAAAAAAAAGACAACGAAGGAGCTATGTTAAAGTACGAAAAAGCCCTCAAACTCTTACCCAAAAACCACATAGATGTTGCACATCTTCACACCAACATGGCCATGTGTTATATGCAATTGGGTCTTGGCGAATACCCTCGAGCGATTAACGAGTGCAATCTTGCTCTGCAAGTTTCGCCGAGGTATAGTAAAGCTCTTCTTAGAAGAGCCAAGTGTTACGAGGCGTTGAATCGGTTGGATTTGGCTATGAGAGATGTTAGAGTTGTTTTGAATGCTGAGCCGAATAATTCAACTGCGTTGGAGATTCTTGATAGTTTGAGGATTACTATGGAGGAGAAAGGGATTACTATTGATGAGACTGAGATTGCTTTGGCTGCTATTCAGCAAGTAGAGCCGCCTGCTGCGCGGTTTAGGAAAGTTGTTAGGGAGAAGATTAATAAGAAGAAGAAAGGGCATAAAGGAGAAGGCGAGGAAGAGCGGAAAAGTAAGAGTGTTGTTGAGAAGAAAGCCACCAATGTTGGAAATGTTAATGTTAATGTTAAGGTGAAGGGTGAAGATTCGAAGGATAAGGTTACGGATAAGGATAAGGATAGTAACGAAGTTGTTAAGGAAGATAAAGCGGTTACTAGGACTGTGAAGTTGATATATGGAGAGGATATTAGATGGGCGCAGTTGCCGGTGAATTGTAGTATGAAGTTGGTGAGAGATGTGATAAGGGATAGGTATCCGGGGTTAAAGGGTGTTCTTGTGAAGTATAAGGATAGGGAAGGTGATTTGGTTACTATTACTACTACTTCTGAGTTGAGGTTAGCTGAGGGTTGTCATGTGCTTGGTTCGATCCGGCTGTATATTACGCAGGTGGATTTGGATATGGAGCCGTGTTATGATGAAAAGATGAGTAGTGGTGAGGTGAGAGGGGAGAATGGGGTTGGAGAAGGAGATAAGGGTGTTGGGGCTAATAGGATGATTACTGTGGAGGAATGGCTTGTTCAGTTTGCGAGGCTGTTTAAGAACCATGTTGGGTTTGATTCTGATTCGTATCTGGATATTCATGAGGTTGGGATGAAGATATATTCGGAGGCGATGGAGGATTCAGTGACGAATGATGGTGCTCAAGAGCTGTTTGATATTGCTGCGGATAAGTTTCAAGAGATGGCTGCTTTGGCTTTGTTTAATTGGGGGAATGTTCACTTGTCTAAGGCGAGGAGGCGAGTGCCTTTTCAGGAAGACGGGTCGAGAGAAGCATCTCTCGAGCATGTTAAAGCTGGATATGAATGGGCTACGAAGGAGTATAAGGAAGCAGAAATGAGATACGAAGAATCTGTGAAAATCAAACCGGACTTCTACGAGGGACTCCTAGCTCTTGGGTATCAACAGTTTGAGCAAGCAAAGCTATGCTGGTGTTATTTAATTGTAAACAATAAGAATATCGAAGTTGGCCCTTTTGAGGAGGTTCTGCAGCTCTATAACAAGGCAGAGGACAGCATGGAAAAAGGGATGTTGATGTGGGAGGAGATTGAGGAACAGCGTTTGAATGGACTCTCTAAGTTAGATAAATATAATGCACAGTTAGAGAAAATGGGTTTGCACGGTCTTTTCAGGGATGTTTCTTCTGAAGAAGCTGAAGATCAGGCTTCAAAGATGAGGATACAGATATACCTTTTATGGGGCACCTTATTGTATGAGCGTTCTGTTGTGGAATTTAAGCTAGGACTACCAACATGGGAGGAATGTTTGGAGGTTGCTGTTGAAAAGTTTGAACTAGCCGGAGCTTCTACAACTGACATTGGTGTCATGATAAAGAACCATTGTTCTAATGAAACAGCAATGGAAGGGTTCAAAATCGATGACATAGTGCAAGCATGGAATGAGATGTATGATGGGTGGCAGTTTGATGTTCCAACTTTCCGACTCGAACCATTGTTTCGTCGGCGTGTTCCAAGACTCCATTACATCCTGGAACAATTTTAA

Protein sequence

>Msa0101020

MGKPAGKKKEHESPKSNNNKNKGYERSTSFDDDTAMFINMSQELREEGNKLFFQKKDNEGAMLKYEKALKLLPKNHIDVAHLHTNMAMCYMQLGLGEYPRAINECNLALQVSPRYSKALLRRAKCYEALNRLDLAMRDVRVVLNAEPNNSTALEILDSLRITMEEKGITIDETEIALAAIQQVEPPAARFRKVVREKINKKKKGHKGEGEEERKSKSVVEKKATNVGNVNVNVKVKGEDSKDKVTDKDKDSNEVVKEDKAVTRTVKLIYGEDIRWAQLPVNCSMKLVRDVIRDRYPGLKGVLVKYKDREGDLVTITTTSELRLAEGCHVLGSIRLYITQVDLDMEPCYDEKMSSGEVRGENGVGEGDKGVGANRMITVEEWLVQFARLFKNHVGFDSDSYLDIHEVGMKIYSEAMEDSVTNDGAQELFDIAADKFQEMAALALFNWGNVHLSKARRRVPFQEDGSREASLEHVKAGYEWATKEYKEAEMRYEESVKIKPDFYEGLLALGYQQFEQAKLCWCYLIVNNKNIEVGPFEEVLQLYNKAEDSMEKGMLMWEEIEEQRLNGLSKLDKYNAQLEKMGLHGLFRDVSSEEAEDQASKMRIQIYLLWGTLLYERSVVEFKLGLPTWEECLEVAVEKFELAGASTTDIGVMIKNHCSNETAMEGFKIDDIVQAWNEMYDGWQFDVPTFRLEPLFRRRVPRLHYILEQF*