AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0376770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0376770 MtrunA17_Chr8g0393061 38.028 142 62 4 80 196 152 292 5.74e-20 85.1
Msa0376770 MtrunA17_Chr5g0402101 31.963 219 122 9 1 196 1 215 4.74e-18 78.6
Msa0376770 MtrunA17_Chr8g0349601 41.322 121 57 4 88 196 162 280 1.92e-17 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0376770 AT5G06000.1 45.794 107 44 3 102 195 181 286 5.19e-18 80.5
Msa0376770 AT3G11400.1 45.370 108 45 4 102 196 188 294 5.96e-18 80.1
Msa0376770 AT3G11400.2 45.370 108 45 4 102 196 215 321 1.09e-17 79.7

Find 72 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATATATGATTCGGTATT+TGG 0.072628 3_1:+77044183 None:intergenic
AAATGCAATCGAGAAGTTTA+AGG 0.247977 3_1:-77043945 Msa0376770:CDS
TCTGGCCTTACTTGGTTCTT+TGG 0.248015 3_1:-77043115 Msa0376770:CDS
GATTCGATCGGAGGATTTAT+TGG 0.268748 3_1:+77044062 None:intergenic
GCTGAACTCTGCCGTCCTTA+TGG 0.275421 3_1:-77043175 Msa0376770:CDS
AGTATTCGTTCATGCCTCTT+AGG 0.341150 3_1:+77043006 None:intergenic
TTGGTAGGAGGAGGAGATTT+GGG 0.343798 3_1:+77043902 None:intergenic
TGACAACGCTAATTCTGTTC+TGG 0.345295 3_1:-77043239 Msa0376770:CDS
TTTGGTAGGAGGAGGAGATT+TGG 0.345311 3_1:+77043901 None:intergenic
ATATATATGATTCGGTATTT+GGG 0.362623 3_1:+77044184 None:intergenic
ATTCGATCGGAGGATTTATT+GGG 0.381915 3_1:+77044063 None:intergenic
TTCAAATGCCAATCGATACT+TGG 0.387434 3_1:-77042938 Msa0376770:three_prime_UTR
ATCAAGAGTCTGGCCTTACT+TGG 0.397071 3_1:-77043123 Msa0376770:CDS
CGTCGTAAGGAACTCTGATT+TGG 0.401539 3_1:-77043197 Msa0376770:CDS
TTAAGAAAAGCTTTGAAAGA+TGG 0.438037 3_1:-77044035 Msa0376770:exon
AGGAGGAGATTTGGGTTCAG+AGG 0.452700 3_1:+77043910 None:intergenic
TGTTGTGGAATTGAATTGAA+TGG 0.454164 3_1:+77044127 None:intergenic
AGAATTAGCGTTGTCAAGAT+AGG 0.456091 3_1:+77043246 None:intergenic
TATGTACCTACAGTCAAGAT+TGG 0.456722 3_1:-77043322 Msa0376770:CDS
GCTTTGTTAGCTTTGCCACC+AGG 0.462501 3_1:-77043093 Msa0376770:CDS
AATGCAATCGAGAAGTTTAA+GGG 0.473415 3_1:-77043944 Msa0376770:CDS
GCTTTGTATAGAATTTAGTC+TGG 0.481052 3_1:-77042884 Msa0376770:three_prime_UTR
CGAGGACAATGTATTGCAAC+CGG 0.485814 3_1:+77043370 None:intergenic
GTTGCTATTGATCAAGAGTC+TGG 0.486644 3_1:-77043133 Msa0376770:CDS
AAGGAAGGATATATATGATT+CGG 0.486706 3_1:+77044176 None:intergenic
CTTGACTGTAGGTACATACT+CGG 0.492126 3_1:+77043327 None:intergenic
GAAGATTTCCAAGTATCGAT+TGG 0.493031 3_1:+77042930 None:intergenic
CCCGAAAACAACCTCCTAAG+AGG 0.493607 3_1:-77043020 Msa0376770:exon
CTTAACTTCGATTCGATCGG+AGG 0.499983 3_1:+77044053 None:intergenic
ACCTCTCCTTCGACGTCGTA+AGG 0.500333 3_1:-77043210 Msa0376770:CDS
ACAAAAGCTTCCATAAACTT+AGG 0.510191 3_1:+77043286 None:intergenic
GTTCCCTTAACTGAAACATC+AGG 0.513610 3_1:-77044232 None:intergenic
CAAAGCCAAAGAACCAAGTA+AGG 0.522006 3_1:+77043110 None:intergenic
ATCAATAGCAACATCGGCAC+TGG 0.526411 3_1:+77043144 None:intergenic
ATGTACCTACAGTCAAGATT+GGG 0.531893 3_1:-77043321 Msa0376770:CDS
GAACAAAAGAAAATTTACTC+TGG 0.533155 3_1:-77042843 Msa0376770:three_prime_UTR
GAGACGGTCGTGATCTCGAC+CGG 0.543343 3_1:-77043976 Msa0376770:CDS
TTTCTTAACTTCGATTCGAT+CGG 0.545705 3_1:+77044050 None:intergenic
GAGCGTAGTTTGGTAGGAGG+AGG 0.548480 3_1:+77043893 None:intergenic
TTGACTGTAGGTACATACTC+GGG 0.548633 3_1:+77043328 None:intergenic
TTCACCGTCGCTGTCGCTGT+CGG 0.556222 3_1:+77043817 None:intergenic
ATTCGTTCATGCCTCTTAGG+AGG 0.559458 3_1:+77043009 None:intergenic
TATTGTGCGTGCGTTTGTTG+TGG 0.564507 3_1:+77044112 None:intergenic
GCACTGGTCACATTTCCATA+AGG 0.565262 3_1:+77043160 None:intergenic
TCCTTACGACGTCGAAGGAG+AGG 0.565857 3_1:+77043209 None:intergenic
CAAATGATGACTTCACATGT+AGG 0.566323 3_1:+77042968 None:intergenic
CTTTGTTAGCTTTGCCACCA+GGG 0.571161 3_1:-77043092 Msa0376770:CDS
ACAATCTCGATGGAGTTCAA+TGG 0.571716 3_1:-77043048 Msa0376770:CDS
TTCCTTCGCAAAACAAAACA+AGG 0.575438 3_1:-77044159 Msa0376770:five_prime_UTR
AACAATGAGCGTAGTTTGGT+AGG 0.576703 3_1:+77043887 None:intergenic
AGCATTCTGAGCATCGTCCC+TGG 0.580578 3_1:+77043075 None:intergenic
GTGAACTCACGGTGTGGAGA+CGG 0.584086 3_1:-77043992 Msa0376770:CDS
AGAGTTCCTTACGACGTCGA+AGG 0.585755 3_1:+77043204 None:intergenic
AAATGATGACTTCACATGTA+GGG 0.586477 3_1:+77042969 None:intergenic
GGCTGCGATTGAGAGAGAAC+TGG 0.594127 3_1:-77044014 Msa0376770:CDS
AATGCTATTAACAATCTCGA+TGG 0.598791 3_1:-77043058 Msa0376770:CDS
TGACTGTAGGTACATACTCG+GGG 0.600362 3_1:+77043329 None:intergenic
CTCTTGATCAATAGCAACAT+CGG 0.606132 3_1:+77043138 None:intergenic
AGAGAGAACTGGTGAACTCA+CGG 0.607110 3_1:-77044003 Msa0376770:CDS
AAACTCCCAATCTTGACTGT+AGG 0.615432 3_1:+77043316 None:intergenic
TACGACGTCGAAGGAGAGGT+CGG 0.621329 3_1:+77043213 None:intergenic
TGGTCACATTTCCATAAGGA+CGG 0.636508 3_1:+77043164 None:intergenic
AATGAGCGTAGTTTGGTAGG+AGG 0.640190 3_1:+77043890 None:intergenic
GACTGTAGGTACATACTCGG+GGG 0.640689 3_1:+77043330 None:intergenic
TGTCGGTGTCGATGCTGTCG+AGG 0.641023 3_1:+77043834 None:intergenic
CGATGCTGTCGAGGTCGCTG+TGG 0.657765 3_1:+77043843 None:intergenic
GACACCGACAGCGACAGCGA+CGG 0.673613 3_1:-77043821 Msa0376770:CDS
GAACTGGTGAACTCACGGTG+TGG 0.694794 3_1:-77043998 Msa0376770:CDS
ATTCTGAGCATCGTCCCTGG+TGG 0.711419 3_1:+77043078 None:intergenic
GCAATCGAGAAGTTTAAGGG+CGG 0.727442 3_1:-77043941 Msa0376770:CDS
ACTGTAGGTACATACTCGGG+GGG 0.731660 3_1:+77043331 None:intergenic
GGTTGTTGACAAGTACATCG+AGG 0.785440 3_1:+77043352 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAAAATTGAATGTAAT+AGG + chr3_1:77043282-77043301 None:intergenic 10.0%
!! TATTAAAAATTGAATGTAAT+AGG + chr3_1:77043282-77043301 None:intergenic 10.0%
!! AATTCAATTCATTATATTGT+TGG + chr3_1:77043449-77043468 None:intergenic 15.0%
!! AATTCAATTCATTATATTGT+TGG + chr3_1:77043449-77043468 None:intergenic 15.0%
!! TCAGGTTATAACTAAAAATA+TGG - chr3_1:77042832-77042851 Msa0376770:three_prime_UTR 20.0%
!! CAGGTTATAACTAAAAATAT+GGG - chr3_1:77042833-77042852 Msa0376770:three_prime_UTR 20.0%
!!! ATATATATGATTCGGTATTT+GGG + chr3_1:77042865-77042884 None:intergenic 20.0%
!! TTGAATGTAATAGGAAAAAA+GGG + chr3_1:77043273-77043292 None:intergenic 20.0%
!! ATTGAATGTAATAGGAAAAA+AGG + chr3_1:77043274-77043293 None:intergenic 20.0%
!!! AACAATATTGAAATTTGCTT+AGG + chr3_1:77043319-77043338 None:intergenic 20.0%
!! TCAGGTTATAACTAAAAATA+TGG - chr3_1:77042832-77042851 Msa0376770:three_prime_UTR 20.0%
!! CAGGTTATAACTAAAAATAT+GGG - chr3_1:77042833-77042852 Msa0376770:three_prime_UTR 20.0%
!!! ATATATATGATTCGGTATTT+GGG + chr3_1:77042865-77042884 None:intergenic 20.0%
!! TTGAATGTAATAGGAAAAAA+GGG + chr3_1:77043273-77043292 None:intergenic 20.0%
!! ATTGAATGTAATAGGAAAAA+AGG + chr3_1:77043274-77043293 None:intergenic 20.0%
!!! AACAATATTGAAATTTGCTT+AGG + chr3_1:77043319-77043338 None:intergenic 20.0%
!!! GATATATATGATTCGGTATT+TGG + chr3_1:77042866-77042885 None:intergenic 25.0%
! AAGGAAGGATATATATGATT+CGG + chr3_1:77042873-77042892 None:intergenic 25.0%
!!! AGGATTTATTGGGTTATTTT+TGG + chr3_1:77042976-77042995 None:intergenic 25.0%
!!! TTAAGAAAAGCTTTGAAAGA+TGG - chr3_1:77043011-77043030 Msa0376770:exon 25.0%
! TGAATGTAATAGGAAAAAAG+GGG + chr3_1:77043272-77043291 None:intergenic 25.0%
! AATTCATTATATTGTTGGAC+TGG + chr3_1:77043444-77043463 None:intergenic 25.0%
! TTAATCTCATTTGTATCTAC+AGG - chr3_1:77043563-77043582 Msa0376770:intron 25.0%
! GAACAAAAGAAAATTTACTC+TGG - chr3_1:77044203-77044222 Msa0376770:five_prime_UTR 25.0%
! TTAGTTATAACCTGATGTTT+CGG + chr3_1:77042827-77042846 None:intergenic 25.0%
!!! GATATATATGATTCGGTATT+TGG + chr3_1:77042866-77042885 None:intergenic 25.0%
! AAGGAAGGATATATATGATT+CGG + chr3_1:77042873-77042892 None:intergenic 25.0%
!!! AGGATTTATTGGGTTATTTT+TGG + chr3_1:77042976-77042995 None:intergenic 25.0%
!!! TTAAGAAAAGCTTTGAAAGA+TGG - chr3_1:77043011-77043030 Msa0376770:exon 25.0%
! TGAATGTAATAGGAAAAAAG+GGG + chr3_1:77043272-77043291 None:intergenic 25.0%
! AATTCATTATATTGTTGGAC+TGG + chr3_1:77043444-77043463 None:intergenic 25.0%
! TTAATCTCATTTGTATCTAC+AGG - chr3_1:77043563-77043582 Msa0376770:intron 25.0%
! GAACAAAAGAAAATTTACTC+TGG - chr3_1:77044203-77044222 Msa0376770:five_prime_UTR 25.0%
TGTTGTGGAATTGAATTGAA+TGG + chr3_1:77042922-77042941 None:intergenic 30.0%
! TTTCTTAACTTCGATTCGAT+CGG + chr3_1:77042999-77043018 None:intergenic 30.0%
AAATGCAATCGAGAAGTTTA+AGG - chr3_1:77043101-77043120 Msa0376770:CDS 30.0%
AATGCAATCGAGAAGTTTAA+GGG - chr3_1:77043102-77043121 Msa0376770:CDS 30.0%
AAAAAACAATGAGCGTAGTT+TGG + chr3_1:77043166-77043185 None:intergenic 30.0%
TGTTGAAAACTCGTTAAAGA+TGG - chr3_1:77043347-77043366 Msa0376770:CDS 30.0%
TCATTTGTATCTACAGGTTT+CGG - chr3_1:77043569-77043588 Msa0376770:intron 30.0%
ATAGTGATATTAACCCACTA+GGG + chr3_1:77043600-77043619 None:intergenic 30.0%
AATAGTGATATTAACCCACT+AGG + chr3_1:77043601-77043620 None:intergenic 30.0%
! TTTTCACGTACCTAAGTTTA+TGG - chr3_1:77043750-77043769 Msa0376770:intron 30.0%
ACAAAAGCTTCCATAAACTT+AGG + chr3_1:77043763-77043782 None:intergenic 30.0%
! TAAGTTTATGGAAGCTTTTG+TGG - chr3_1:77043762-77043781 Msa0376770:intron 30.0%
AATGCTATTAACAATCTCGA+TGG - chr3_1:77043988-77044007 Msa0376770:CDS 30.0%
AAATGATGACTTCACATGTA+GGG + chr3_1:77044080-77044099 None:intergenic 30.0%
GCTTTGTATAGAATTTAGTC+TGG - chr3_1:77044162-77044181 Msa0376770:five_prime_UTR 30.0%
TGTTGTGGAATTGAATTGAA+TGG + chr3_1:77042922-77042941 None:intergenic 30.0%
! TTTCTTAACTTCGATTCGAT+CGG + chr3_1:77042999-77043018 None:intergenic 30.0%
AAATGCAATCGAGAAGTTTA+AGG - chr3_1:77043101-77043120 Msa0376770:CDS 30.0%
AATGCAATCGAGAAGTTTAA+GGG - chr3_1:77043102-77043121 Msa0376770:CDS 30.0%
AAAAAACAATGAGCGTAGTT+TGG + chr3_1:77043166-77043185 None:intergenic 30.0%
TGTTGAAAACTCGTTAAAGA+TGG - chr3_1:77043347-77043366 Msa0376770:CDS 30.0%
TCATTTGTATCTACAGGTTT+CGG - chr3_1:77043569-77043588 Msa0376770:intron 30.0%
ATAGTGATATTAACCCACTA+GGG + chr3_1:77043600-77043619 None:intergenic 30.0%
AATAGTGATATTAACCCACT+AGG + chr3_1:77043601-77043620 None:intergenic 30.0%
! TTTTCACGTACCTAAGTTTA+TGG - chr3_1:77043750-77043769 Msa0376770:intron 30.0%
ACAAAAGCTTCCATAAACTT+AGG + chr3_1:77043763-77043782 None:intergenic 30.0%
! TAAGTTTATGGAAGCTTTTG+TGG - chr3_1:77043762-77043781 Msa0376770:intron 30.0%
AATGCTATTAACAATCTCGA+TGG - chr3_1:77043988-77044007 Msa0376770:CDS 30.0%
AAATGATGACTTCACATGTA+GGG + chr3_1:77044080-77044099 None:intergenic 30.0%
GCTTTGTATAGAATTTAGTC+TGG - chr3_1:77044162-77044181 Msa0376770:five_prime_UTR 30.0%
!!! TTGTTTTGTTTTGCGAAGGA+AGG + chr3_1:77042888-77042907 None:intergenic 35.0%
TTCCTTCGCAAAACAAAACA+AGG - chr3_1:77042887-77042906 Msa0376770:three_prime_UTR 35.0%
!!! AACCTTGTTTTGTTTTGCGA+AGG + chr3_1:77042892-77042911 None:intergenic 35.0%
ACGCACAATAACAAAAAACC+AGG - chr3_1:77042947-77042966 Msa0376770:three_prime_UTR 35.0%
CGCACAATAACAAAAAACCA+GGG - chr3_1:77042948-77042967 Msa0376770:three_prime_UTR 35.0%
ATTCGATCGGAGGATTTATT+GGG + chr3_1:77042986-77043005 None:intergenic 35.0%
!!! GCTCATTGTTTTTTGACGAA+TGG - chr3_1:77043172-77043191 Msa0376770:CDS 35.0%
TTAGTAAAAGCCAACTAGGA+TGG + chr3_1:77043421-77043440 None:intergenic 35.0%
CATTGTAGTCTGTGATACAT+CGG + chr3_1:77043516-77043535 None:intergenic 35.0%
TATGTACCTACAGTCAAGAT+TGG - chr3_1:77043724-77043743 Msa0376770:intron 35.0%
ATGTACCTACAGTCAAGATT+GGG - chr3_1:77043725-77043744 Msa0376770:intron 35.0%
! GCTTTTGTGGATAAGATGAT+TGG - chr3_1:77043775-77043794 Msa0376770:intron 35.0%
AGAATTAGCGTTGTCAAGAT+AGG + chr3_1:77043803-77043822 None:intergenic 35.0%
CTCTTGATCAATAGCAACAT+CGG + chr3_1:77043911-77043930 None:intergenic 35.0%
CAAATGATGACTTCACATGT+AGG + chr3_1:77044081-77044100 None:intergenic 35.0%
TTCAAATGCCAATCGATACT+TGG - chr3_1:77044108-77044127 Msa0376770:five_prime_UTR 35.0%
!! GAAGATTTCCAAGTATCGAT+TGG + chr3_1:77044119-77044138 None:intergenic 35.0%
!!! TTGTTTTGTTTTGCGAAGGA+AGG + chr3_1:77042888-77042907 None:intergenic 35.0%
TTCCTTCGCAAAACAAAACA+AGG - chr3_1:77042887-77042906 Msa0376770:three_prime_UTR 35.0%
!!! AACCTTGTTTTGTTTTGCGA+AGG + chr3_1:77042892-77042911 None:intergenic 35.0%
ACGCACAATAACAAAAAACC+AGG - chr3_1:77042947-77042966 Msa0376770:three_prime_UTR 35.0%
CGCACAATAACAAAAAACCA+GGG - chr3_1:77042948-77042967 Msa0376770:three_prime_UTR 35.0%
ATTCGATCGGAGGATTTATT+GGG + chr3_1:77042986-77043005 None:intergenic 35.0%
!!! GCTCATTGTTTTTTGACGAA+TGG - chr3_1:77043172-77043191 Msa0376770:CDS 35.0%
TTAGTAAAAGCCAACTAGGA+TGG + chr3_1:77043421-77043440 None:intergenic 35.0%
CATTGTAGTCTGTGATACAT+CGG + chr3_1:77043516-77043535 None:intergenic 35.0%
TATGTACCTACAGTCAAGAT+TGG - chr3_1:77043724-77043743 Msa0376770:intron 35.0%
ATGTACCTACAGTCAAGATT+GGG - chr3_1:77043725-77043744 Msa0376770:intron 35.0%
! GCTTTTGTGGATAAGATGAT+TGG - chr3_1:77043775-77043794 Msa0376770:intron 35.0%
AGAATTAGCGTTGTCAAGAT+AGG + chr3_1:77043803-77043822 None:intergenic 35.0%
CTCTTGATCAATAGCAACAT+CGG + chr3_1:77043911-77043930 None:intergenic 35.0%
CAAATGATGACTTCACATGT+AGG + chr3_1:77044081-77044100 None:intergenic 35.0%
TTCAAATGCCAATCGATACT+TGG - chr3_1:77044108-77044127 Msa0376770:five_prime_UTR 35.0%
!! GAAGATTTCCAAGTATCGAT+TGG + chr3_1:77044119-77044138 None:intergenic 35.0%
!!! TTGGGTTATTTTTGGAACCC+TGG + chr3_1:77042968-77042987 None:intergenic 40.0%
GATTCGATCGGAGGATTTAT+TGG + chr3_1:77042987-77043006 None:intergenic 40.0%
! TTCTCGATTGCATTTTCGTC+AGG + chr3_1:77043096-77043115 None:intergenic 40.0%
AACAATGAGCGTAGTTTGGT+AGG + chr3_1:77043162-77043181 None:intergenic 40.0%
CTGGTTAGTAAAAGCCAACT+AGG + chr3_1:77043425-77043444 None:intergenic 40.0%
TAGTCTGTGATACATCGGAA+CGG + chr3_1:77043511-77043530 None:intergenic 40.0%
TGATATTAACCCACTAGGGT+TGG + chr3_1:77043596-77043615 None:intergenic 40.0%
TTGACTGTAGGTACATACTC+GGG + chr3_1:77043721-77043740 None:intergenic 40.0%
CTTGACTGTAGGTACATACT+CGG + chr3_1:77043722-77043741 None:intergenic 40.0%
AAACTCCCAATCTTGACTGT+AGG + chr3_1:77043733-77043752 None:intergenic 40.0%
TGACAACGCTAATTCTGTTC+TGG - chr3_1:77043807-77043826 Msa0376770:intron 40.0%
TGGTCACATTTCCATAAGGA+CGG + chr3_1:77043885-77043904 None:intergenic 40.0%
! GTTGCTATTGATCAAGAGTC+TGG - chr3_1:77043913-77043932 Msa0376770:CDS 40.0%
CAAAGCCAAAGAACCAAGTA+AGG + chr3_1:77043939-77043958 None:intergenic 40.0%
ACAATCTCGATGGAGTTCAA+TGG - chr3_1:77043998-77044017 Msa0376770:CDS 40.0%
AGTATTCGTTCATGCCTCTT+AGG + chr3_1:77044043-77044062 None:intergenic 40.0%
!!! TTGGGTTATTTTTGGAACCC+TGG + chr3_1:77042968-77042987 None:intergenic 40.0%
GATTCGATCGGAGGATTTAT+TGG + chr3_1:77042987-77043006 None:intergenic 40.0%
! TTCTCGATTGCATTTTCGTC+AGG + chr3_1:77043096-77043115 None:intergenic 40.0%
AACAATGAGCGTAGTTTGGT+AGG + chr3_1:77043162-77043181 None:intergenic 40.0%
CTGGTTAGTAAAAGCCAACT+AGG + chr3_1:77043425-77043444 None:intergenic 40.0%
TAGTCTGTGATACATCGGAA+CGG + chr3_1:77043511-77043530 None:intergenic 40.0%
TGATATTAACCCACTAGGGT+TGG + chr3_1:77043596-77043615 None:intergenic 40.0%
TTGACTGTAGGTACATACTC+GGG + chr3_1:77043721-77043740 None:intergenic 40.0%
CTTGACTGTAGGTACATACT+CGG + chr3_1:77043722-77043741 None:intergenic 40.0%
AAACTCCCAATCTTGACTGT+AGG + chr3_1:77043733-77043752 None:intergenic 40.0%
TGACAACGCTAATTCTGTTC+TGG - chr3_1:77043807-77043826 Msa0376770:intron 40.0%
TGGTCACATTTCCATAAGGA+CGG + chr3_1:77043885-77043904 None:intergenic 40.0%
! GTTGCTATTGATCAAGAGTC+TGG - chr3_1:77043913-77043932 Msa0376770:CDS 40.0%
CAAAGCCAAAGAACCAAGTA+AGG + chr3_1:77043939-77043958 None:intergenic 40.0%
ACAATCTCGATGGAGTTCAA+TGG - chr3_1:77043998-77044017 Msa0376770:CDS 40.0%
AGTATTCGTTCATGCCTCTT+AGG + chr3_1:77044043-77044062 None:intergenic 40.0%
! TATTGTGCGTGCGTTTGTTG+TGG + chr3_1:77042937-77042956 None:intergenic 45.0%
! CTTAACTTCGATTCGATCGG+AGG + chr3_1:77042996-77043015 None:intergenic 45.0%
AGAGAGAACTGGTGAACTCA+CGG - chr3_1:77043043-77043062 Msa0376770:CDS 45.0%
GCAATCGAGAAGTTTAAGGG+CGG - chr3_1:77043105-77043124 Msa0376770:CDS 45.0%
TTGGTAGGAGGAGGAGATTT+GGG + chr3_1:77043147-77043166 None:intergenic 45.0%
TTTGGTAGGAGGAGGAGATT+TGG + chr3_1:77043148-77043167 None:intergenic 45.0%
AATGAGCGTAGTTTGGTAGG+AGG + chr3_1:77043159-77043178 None:intergenic 45.0%
ATCCCACCGAAATTGACATC+AGG - chr3_1:77043375-77043394 Msa0376770:CDS 45.0%
TCCCACCGAAATTGACATCA+GGG - chr3_1:77043376-77043395 Msa0376770:intron 45.0%
TAAGCCAACACCATCCTAGT+TGG - chr3_1:77043408-77043427 Msa0376770:intron 45.0%
AAAGCCAACTAGGATGGTGT+TGG + chr3_1:77043415-77043434 None:intergenic 45.0%
CTGTAGATACAAACAGGGAC+AGG + chr3_1:77043654-77043673 None:intergenic 45.0%
CTGTCCCTGTTTGTATCTAC+AGG - chr3_1:77043652-77043671 Msa0376770:intron 45.0%
CGGACCTGTAGATACAAACA+GGG + chr3_1:77043659-77043678 None:intergenic 45.0%
CCTGTTTGTATCTACAGGTC+CGG - chr3_1:77043657-77043676 Msa0376770:intron 45.0%
CGAGGACAATGTATTGCAAC+CGG + chr3_1:77043679-77043698 None:intergenic 45.0%
GGTTGTTGACAAGTACATCG+AGG + chr3_1:77043697-77043716 None:intergenic 45.0%
TGACTGTAGGTACATACTCG+GGG + chr3_1:77043720-77043739 None:intergenic 45.0%
CGTCGTAAGGAACTCTGATT+TGG - chr3_1:77043849-77043868 Msa0376770:CDS 45.0%
! GCACTGGTCACATTTCCATA+AGG + chr3_1:77043889-77043908 None:intergenic 45.0%
ATCAATAGCAACATCGGCAC+TGG + chr3_1:77043905-77043924 None:intergenic 45.0%
!! ATCAAGAGTCTGGCCTTACT+TGG - chr3_1:77043923-77043942 Msa0376770:CDS 45.0%
!! TCTGGCCTTACTTGGTTCTT+TGG - chr3_1:77043931-77043950 Msa0376770:CDS 45.0%
!! CTTTGTTAGCTTTGCCACCA+GGG - chr3_1:77043954-77043973 Msa0376770:CDS 45.0%
!! CTCTTAGGAGGTTGTTTTCG+GGG + chr3_1:77044028-77044047 None:intergenic 45.0%
!! CCTCTTAGGAGGTTGTTTTC+GGG + chr3_1:77044029-77044048 None:intergenic 45.0%
!! GCCTCTTAGGAGGTTGTTTT+CGG + chr3_1:77044030-77044049 None:intergenic 45.0%
ATTCGTTCATGCCTCTTAGG+AGG + chr3_1:77044040-77044059 None:intergenic 45.0%
! TATTGTGCGTGCGTTTGTTG+TGG + chr3_1:77042937-77042956 None:intergenic 45.0%
! CTTAACTTCGATTCGATCGG+AGG + chr3_1:77042996-77043015 None:intergenic 45.0%
AGAGAGAACTGGTGAACTCA+CGG - chr3_1:77043043-77043062 Msa0376770:CDS 45.0%
GCAATCGAGAAGTTTAAGGG+CGG - chr3_1:77043105-77043124 Msa0376770:CDS 45.0%
TTGGTAGGAGGAGGAGATTT+GGG + chr3_1:77043147-77043166 None:intergenic 45.0%
TTTGGTAGGAGGAGGAGATT+TGG + chr3_1:77043148-77043167 None:intergenic 45.0%
AATGAGCGTAGTTTGGTAGG+AGG + chr3_1:77043159-77043178 None:intergenic 45.0%
ATCCCACCGAAATTGACATC+AGG - chr3_1:77043375-77043394 Msa0376770:CDS 45.0%
TCCCACCGAAATTGACATCA+GGG - chr3_1:77043376-77043395 Msa0376770:intron 45.0%
TAAGCCAACACCATCCTAGT+TGG - chr3_1:77043408-77043427 Msa0376770:intron 45.0%
AAAGCCAACTAGGATGGTGT+TGG + chr3_1:77043415-77043434 None:intergenic 45.0%
CTGTAGATACAAACAGGGAC+AGG + chr3_1:77043654-77043673 None:intergenic 45.0%
CTGTCCCTGTTTGTATCTAC+AGG - chr3_1:77043652-77043671 Msa0376770:intron 45.0%
CGGACCTGTAGATACAAACA+GGG + chr3_1:77043659-77043678 None:intergenic 45.0%
CCTGTTTGTATCTACAGGTC+CGG - chr3_1:77043657-77043676 Msa0376770:intron 45.0%
CGAGGACAATGTATTGCAAC+CGG + chr3_1:77043679-77043698 None:intergenic 45.0%
GGTTGTTGACAAGTACATCG+AGG + chr3_1:77043697-77043716 None:intergenic 45.0%
TGACTGTAGGTACATACTCG+GGG + chr3_1:77043720-77043739 None:intergenic 45.0%
CGTCGTAAGGAACTCTGATT+TGG - chr3_1:77043849-77043868 Msa0376770:CDS 45.0%
! GCACTGGTCACATTTCCATA+AGG + chr3_1:77043889-77043908 None:intergenic 45.0%
ATCAATAGCAACATCGGCAC+TGG + chr3_1:77043905-77043924 None:intergenic 45.0%
!! ATCAAGAGTCTGGCCTTACT+TGG - chr3_1:77043923-77043942 Msa0376770:CDS 45.0%
!! TCTGGCCTTACTTGGTTCTT+TGG - chr3_1:77043931-77043950 Msa0376770:CDS 45.0%
!! CTTTGTTAGCTTTGCCACCA+GGG - chr3_1:77043954-77043973 Msa0376770:CDS 45.0%
!! CTCTTAGGAGGTTGTTTTCG+GGG + chr3_1:77044028-77044047 None:intergenic 45.0%
!! CCTCTTAGGAGGTTGTTTTC+GGG + chr3_1:77044029-77044048 None:intergenic 45.0%
!! GCCTCTTAGGAGGTTGTTTT+CGG + chr3_1:77044030-77044049 None:intergenic 45.0%
ATTCGTTCATGCCTCTTAGG+AGG + chr3_1:77044040-77044059 None:intergenic 45.0%
! CGATTGCATTTTCGTCAGGC+CGG + chr3_1:77043092-77043111 None:intergenic 50.0%
! AGGAGGAGATTTGGGTTCAG+AGG + chr3_1:77043139-77043158 None:intergenic 50.0%
CCCCTGATGTCAATTTCGGT+GGG + chr3_1:77043380-77043399 None:intergenic 50.0%
CCCACCGAAATTGACATCAG+GGG - chr3_1:77043377-77043396 Msa0376770:intron 50.0%
CCACCGAAATTGACATCAGG+GGG - chr3_1:77043378-77043397 Msa0376770:intron 50.0%
CCTCCCCCTGATGTCAATTT+CGG + chr3_1:77043384-77043403 None:intergenic 50.0%
CGAAATTGACATCAGGGGGA+GGG - chr3_1:77043382-77043401 Msa0376770:intron 50.0%
CCGGACCTGTAGATACAAAC+AGG + chr3_1:77043660-77043679 None:intergenic 50.0%
ACTGTAGGTACATACTCGGG+GGG + chr3_1:77043718-77043737 None:intergenic 50.0%
GACTGTAGGTACATACTCGG+GGG + chr3_1:77043719-77043738 None:intergenic 50.0%
AGAGTTCCTTACGACGTCGA+AGG + chr3_1:77043845-77043864 None:intergenic 50.0%
!! GCTTTGTTAGCTTTGCCACC+AGG - chr3_1:77043953-77043972 Msa0376770:CDS 50.0%
CCCGAAAACAACCTCCTAAG+AGG - chr3_1:77044026-77044045 Msa0376770:exon 50.0%
! CGATTGCATTTTCGTCAGGC+CGG + chr3_1:77043092-77043111 None:intergenic 50.0%
! AGGAGGAGATTTGGGTTCAG+AGG + chr3_1:77043139-77043158 None:intergenic 50.0%
CCCCTGATGTCAATTTCGGT+GGG + chr3_1:77043380-77043399 None:intergenic 50.0%
CCCACCGAAATTGACATCAG+GGG - chr3_1:77043377-77043396 Msa0376770:intron 50.0%
CCACCGAAATTGACATCAGG+GGG - chr3_1:77043378-77043397 Msa0376770:intron 50.0%
CCTCCCCCTGATGTCAATTT+CGG + chr3_1:77043384-77043403 None:intergenic 50.0%
CGAAATTGACATCAGGGGGA+GGG - chr3_1:77043382-77043401 Msa0376770:intron 50.0%
CCGGACCTGTAGATACAAAC+AGG + chr3_1:77043660-77043679 None:intergenic 50.0%
ACTGTAGGTACATACTCGGG+GGG + chr3_1:77043718-77043737 None:intergenic 50.0%
GACTGTAGGTACATACTCGG+GGG + chr3_1:77043719-77043738 None:intergenic 50.0%
AGAGTTCCTTACGACGTCGA+AGG + chr3_1:77043845-77043864 None:intergenic 50.0%
!! GCTTTGTTAGCTTTGCCACC+AGG - chr3_1:77043953-77043972 Msa0376770:CDS 50.0%
CCCGAAAACAACCTCCTAAG+AGG - chr3_1:77044026-77044045 Msa0376770:exon 50.0%
GGCTGCGATTGAGAGAGAAC+TGG - chr3_1:77043032-77043051 Msa0376770:CDS 55.0%
GAACTGGTGAACTCACGGTG+TGG - chr3_1:77043048-77043067 Msa0376770:CDS 55.0%
GTGAACTCACGGTGTGGAGA+CGG - chr3_1:77043054-77043073 Msa0376770:CDS 55.0%
GAGCGTAGTTTGGTAGGAGG+AGG + chr3_1:77043156-77043175 None:intergenic 55.0%
CCCCCTGATGTCAATTTCGG+TGG + chr3_1:77043381-77043400 None:intergenic 55.0%
CCGAAATTGACATCAGGGGG+AGG - chr3_1:77043381-77043400 Msa0376770:intron 55.0%
AGGTTTCGGTCCAACCCTAG+TGG - chr3_1:77043583-77043602 Msa0376770:intron 55.0%
GGTTTCGGTCCAACCCTAGT+GGG - chr3_1:77043584-77043603 Msa0376770:intron 55.0%
TACGACGTCGAAGGAGAGGT+CGG + chr3_1:77043836-77043855 None:intergenic 55.0%
ACCTCTCCTTCGACGTCGTA+AGG - chr3_1:77043836-77043855 Msa0376770:CDS 55.0%
TCCTTACGACGTCGAAGGAG+AGG + chr3_1:77043840-77043859 None:intergenic 55.0%
GCTGAACTCTGCCGTCCTTA+TGG - chr3_1:77043871-77043890 Msa0376770:CDS 55.0%
! ATTCTGAGCATCGTCCCTGG+TGG + chr3_1:77043971-77043990 None:intergenic 55.0%
! AGCATTCTGAGCATCGTCCC+TGG + chr3_1:77043974-77043993 None:intergenic 55.0%
GGCTGCGATTGAGAGAGAAC+TGG - chr3_1:77043032-77043051 Msa0376770:CDS 55.0%
GAACTGGTGAACTCACGGTG+TGG - chr3_1:77043048-77043067 Msa0376770:CDS 55.0%
GTGAACTCACGGTGTGGAGA+CGG - chr3_1:77043054-77043073 Msa0376770:CDS 55.0%
GAGCGTAGTTTGGTAGGAGG+AGG + chr3_1:77043156-77043175 None:intergenic 55.0%
CCCCCTGATGTCAATTTCGG+TGG + chr3_1:77043381-77043400 None:intergenic 55.0%
CCGAAATTGACATCAGGGGG+AGG - chr3_1:77043381-77043400 Msa0376770:intron 55.0%
AGGTTTCGGTCCAACCCTAG+TGG - chr3_1:77043583-77043602 Msa0376770:intron 55.0%
GGTTTCGGTCCAACCCTAGT+GGG - chr3_1:77043584-77043603 Msa0376770:intron 55.0%
TACGACGTCGAAGGAGAGGT+CGG + chr3_1:77043836-77043855 None:intergenic 55.0%
ACCTCTCCTTCGACGTCGTA+AGG - chr3_1:77043836-77043855 Msa0376770:CDS 55.0%
TCCTTACGACGTCGAAGGAG+AGG + chr3_1:77043840-77043859 None:intergenic 55.0%
GCTGAACTCTGCCGTCCTTA+TGG - chr3_1:77043871-77043890 Msa0376770:CDS 55.0%
! ATTCTGAGCATCGTCCCTGG+TGG + chr3_1:77043971-77043990 None:intergenic 55.0%
! AGCATTCTGAGCATCGTCCC+TGG + chr3_1:77043974-77043993 None:intergenic 55.0%
GAGACGGTCGTGATCTCGAC+CGG - chr3_1:77043070-77043089 Msa0376770:CDS 60.0%
!! TGTCGGTGTCGATGCTGTCG+AGG + chr3_1:77043215-77043234 None:intergenic 60.0%
TTCACCGTCGCTGTCGCTGT+CGG + chr3_1:77043232-77043251 None:intergenic 60.0%
GAGACGGTCGTGATCTCGAC+CGG - chr3_1:77043070-77043089 Msa0376770:CDS 60.0%
!! TGTCGGTGTCGATGCTGTCG+AGG + chr3_1:77043215-77043234 None:intergenic 60.0%
TTCACCGTCGCTGTCGCTGT+CGG + chr3_1:77043232-77043251 None:intergenic 60.0%
CGATGCTGTCGAGGTCGCTG+TGG + chr3_1:77043206-77043225 None:intergenic 65.0%
GACACCGACAGCGACAGCGA+CGG - chr3_1:77043225-77043244 Msa0376770:CDS 65.0%
CGACAGCGACGGTGAACGTG+AGG - chr3_1:77043236-77043255 Msa0376770:CDS 65.0%
CGATGCTGTCGAGGTCGCTG+TGG + chr3_1:77043206-77043225 None:intergenic 65.0%
GACACCGACAGCGACAGCGA+CGG - chr3_1:77043225-77043244 Msa0376770:CDS 65.0%
CGACAGCGACGGTGAACGTG+AGG - chr3_1:77043236-77043255 Msa0376770:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 77042826 77044242 77042826 ID=Msa0376770;Name=Msa0376770
chr3_1 mRNA 77042826 77044242 77042826 ID=Msa0376770-mRNA-1;Parent=Msa0376770;Name=Msa0376770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=204|1|1|1|1|1|2|198|197
chr3_1 exon 77042826 77043394 77042826 ID=Msa0376770-mRNA-1:exon:18356;Parent=Msa0376770-mRNA-1
chr3_1 exon 77043816 77044242 77043816 ID=Msa0376770-mRNA-1:exon:18355;Parent=Msa0376770-mRNA-1
chr3_1 five_prime_UTR 77044039 77044242 77044039 ID=Msa0376770-mRNA-1:five_prime_utr;Parent=Msa0376770-mRNA-1
chr3_1 CDS 77043816 77044038 77043816 ID=Msa0376770-mRNA-1:cds;Parent=Msa0376770-mRNA-1
chr3_1 CDS 77043024 77043394 77043024 ID=Msa0376770-mRNA-1:cds;Parent=Msa0376770-mRNA-1
chr3_1 three_prime_UTR 77042826 77043023 77042826 ID=Msa0376770-mRNA-1:three_prime_utr;Parent=Msa0376770-mRNA-1
Gene Sequence

>Msa0376770

ATGGCTGCGATTGAGAGAGAACTGGTGAACTCACGGTGTGGAGACGGTCGTGATCTCGACCGGCCTGACGAAAATGCAATCGAGAAGTTTAAGGGCGGCGACGTCTCCTCTGAACCCAAATCTCCTCCTCCTACCAAACTACGCTCATTGTTTTTTGACGAATGGTTGTCGCTCCACAGCGACCTCGACAGCATCGACACCGACAGCGACAGCGACGGTGAACGTCCGGTTGCAATACATTGTCCTCGATGTACTTGTCAACAACCCCCCGAGTATGTACCTACAGTCAAGATTGGGAGTTTTCACGTACCTAAGTTTATGGAAGCTTTTGTGGATAAGATGATTGGATCCTATCTTGACAACGCTAATTCTGTTCTGGTAACCGACCTCTCCTTCGACGTCGTAAGGAACTCTGATTTGGCTGAACTCTGCCGTCCTTATGGAAATGTGACCAGTGCCGATGTTGCTATTGATCAAGAGTCTGGCCTTACTTGGTTCTTTGGCTTTGTTAGCTTTGCCACCAGGGACGATGCTCAGAATGCTATTAACAATCTCGATGGAGTTCAATGGCTCACCCCGAAAACAACCTCCTAA
Protein sequence

>Msa0376770

MAAIERELVNSRCGDGRDLDRPDENAIEKFKGGDVSSEPKSPPPTKLRSLFFDEWLSLHSDLDSIDTDSDSDGERPVAIHCPRCTCQQPPEYVPTVKIGSFHVPKFMEAFVDKMIGSYLDNANSVLVTDLSFDVVRNSDLAELCRPYGNVTSADVAIDQESGLTWFFGFVSFATRDDAQNAINNLDGVQWLTPKTTS*