AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0740100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0740100 MtrunA17_Chr5g0440321 70.637 361 14 5 1 353 1 277 5.18e-151 426
Msa0740100 MtrunA17_Chr5g0440311 33.908 348 141 11 1 342 1 265 1.06e-34 128
Msa0740100 MtrunA17_Chr5g0440291 35.644 202 111 6 1 185 3 202 1.23e-25 103
Msa0740100 MtrunA17_Chr6g0481571 32.824 131 80 3 1 128 1 126 5.13e-14 69.3
Msa0740100 MtrunA17_Chr8g0356011 29.075 227 131 8 4 226 15 215 1.30e-13 71.6
Msa0740100 MtrunA17_Chr7g0253661 37.209 86 52 1 10 93 8 93 1.96e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0740100 AT2G27140.1 40.252 159 74 5 9 155 10 159 1.96e-26 105
Msa0740100 AT5G20970.1 35.227 176 108 5 10 183 7 178 1.92e-19 87.0

Find 88 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTCTCTTCTTTAGCTTT+TGG 0.144895 5_1:-58917770 Msa0740110:three_prime_UTR
GCTTTCTCTTCTATAGCTTT+TGG 0.148334 5_1:-58918004 Msa0740110:three_prime_UTR
AGCAATAAACTTGGCATCTT+TGG 0.197304 5_1:-58918197 Msa0740110:three_prime_UTR
ATCTTATTGGTCGTGGGACT+TGG 0.235571 5_1:+58918349 Msa0740100:CDS
TTTGGTCTTGGAGCAGCTTC+TGG 0.245115 5_1:-58917710 Msa0740110:three_prime_UTR
TATTTATCCCATTCATGATA+TGG 0.253330 5_1:-58917211 None:intergenic
TTTGGTCTTGGAGCTTCTTC+TGG 0.254733 5_1:-58917932 Msa0740110:three_prime_UTR
TTTAGTCTTGGAGCTTCTTC+TGG 0.262302 5_1:-58917821 Msa0740110:three_prime_UTR
ACAAAGGCATAACTAATAAA+TGG 0.287057 5_1:+58917141 Msa0740100:exon
CTTAACATGCAGGATATAGA+AGG 0.298323 5_1:+58917477 Msa0740100:intron
GTCTTTCACCTATCAGCCTT+AGG 0.314056 5_1:-58917535 None:intergenic
TGTGATGGTTCCTTTGGTCT+TGG 0.318915 5_1:-58917722 Msa0740110:three_prime_UTR
CTTTATTGTAGAAATACATT+TGG 0.339189 5_1:+58918373 Msa0740100:CDS
TGTGGCGATTCCTTTGGTCT+TGG 0.352071 5_1:-58917944 Msa0740110:three_prime_UTR
GGATTTGTTGTTGTTTCCTT+AGG 0.356840 5_1:-58917686 None:intergenic
ATGTGTCATGCTAATCTTAT+TGG 0.363566 5_1:+58918336 Msa0740100:CDS
TCATAGACACGATCAGCTTC+TGG 0.364950 5_1:-58917178 None:intergenic
TGTAGATTTCACTCTCTCTA+AGG 0.366735 5_1:+58918237 Msa0740100:CDS
CTCTAAGGCTCTCTGTAATC+AGG 0.374231 5_1:+58918252 Msa0740100:CDS
TGCGATGGTTCCTTTAGTCT+TGG 0.384700 5_1:-58917833 Msa0740110:three_prime_UTR
AAATACATTTGGATCGTCTC+AGG 0.407520 5_1:+58918384 Msa0740100:CDS
ACAAAAGGTTGATGAGCAAA+AGG 0.416395 5_1:+58917742 Msa0740100:CDS
TGGATCGTCTCAGGGAGAAT+TGG 0.416829 5_1:+58918393 Msa0740100:CDS
ACAAAAGGCTGATGAGCAAA+AGG 0.421804 5_1:+58917964 Msa0740100:CDS
AAACCTGCCCTAAGGCTGAT+AGG 0.427961 5_1:+58917527 Msa0740100:CDS
GCAAAACGTTGATGAACAAA+AGG 0.437399 5_1:+58917853 Msa0740100:CDS
TTGCCTCTTAGTAATTGAAA+TGG 0.441701 5_1:-58917095 None:intergenic
TCTTTCACCTATCAGCCTTA+GGG 0.449497 5_1:-58917534 None:intergenic
CATGCTAATCTTATTGGTCG+TGG 0.455442 5_1:+58918342 Msa0740100:CDS
ACTTTGAACCATATCATGAA+TGG 0.461998 5_1:+58917203 Msa0740100:CDS
CGTTTCACTGTCATGCTACC+AGG 0.466798 5_1:+58917241 Msa0740100:CDS
TCTTAAGAAGTGGAAGAGAT+TGG 0.472099 5_1:+58918309 Msa0740100:CDS
GCGGAGAAAGTGAGGACTAA+TGG 0.476427 5_1:+58918130 Msa0740100:CDS
TGCTAAACTTACTAATCCTA+AGG 0.484970 5_1:+58917670 Msa0740100:CDS
TCTTATTGGTCGTGGGACTT+GGG 0.489096 5_1:+58918350 Msa0740100:CDS
CGCCATCTGTTCTGAAAGGT+TGG 0.490011 5_1:-58917557 None:intergenic
CAGGATGAAAATGAAGCCCT+TGG 0.494161 5_1:+58918271 Msa0740100:CDS
ACCAAAGGAATCGCCACAAA+AGG 0.496416 5_1:+58917949 Msa0740100:CDS
TGACCATTTCAATTACTAAG+AGG 0.508482 5_1:+58917092 Msa0740100:five_prime_UTR
CTTTGAACCATATCATGAAT+GGG 0.508732 5_1:+58917204 Msa0740100:CDS
ATGCTAATCTTATTGGTCGT+GGG 0.509173 5_1:+58918343 Msa0740100:CDS
ATTGTATCATTCTCCTTCTG+TGG 0.509921 5_1:-58918067 Msa0740110:three_prime_UTR
ACCAAAGGAACCATCACAAA+AGG 0.514374 5_1:+58917727 Msa0740100:CDS
CAAATATCAGTACATCAGTA+AGG 0.527580 5_1:-58918439 Msa0740110:three_prime_UTR
TTATTGCTAGATGCAAAACC+AGG 0.528675 5_1:+58918212 Msa0740100:CDS
TTTGCATCTAGCAATAAACT+TGG 0.529793 5_1:-58918206 Msa0740110:three_prime_UTR
CAGTGTGACTGCGACATTTG+AGG 0.530897 5_1:+58917625 Msa0740100:CDS
AGAAGAGAAAGTAGAAACAA+AGG 0.532724 5_1:+58917781 Msa0740100:CDS
CATGAATGGGATAAATATGA+TGG 0.533667 5_1:+58917217 Msa0740100:CDS
AGTGTGACTGCGACATTTGA+GGG 0.535084 5_1:+58917626 Msa0740100:CDS
TCACCTATCAGCCTTAGGGC+AGG 0.538165 5_1:-58917530 None:intergenic
GTGTCATAATCAGAAGGAAT+GGG 0.540119 5_1:-58917599 None:intergenic
GCCCTTGGAAATTCGACCAC+AGG 0.540651 5_1:+58918286 Msa0740100:CDS
GAGAGTGAAATCTACAAGCC+TGG 0.545424 5_1:-58918230 Msa0740110:three_prime_UTR
GGTCTTGGAGCAGCTTCTGG+TGG 0.547053 5_1:-58917707 Msa0740110:three_prime_UTR
AGTCTTGGAGCTTCTTCTGG+TGG 0.547838 5_1:-58917818 Msa0740110:three_prime_UTR
CAAAAGGCTGATGAGCAAAA+GGG 0.548506 5_1:+58917965 Msa0740100:CDS
CAAAAGGTTGATGAGCAAAA+GGG 0.551402 5_1:+58917743 Msa0740100:CDS
AGAAGAAGCTCCAAGACTAA+AGG 0.551790 5_1:+58917823 Msa0740100:CDS
GACCTGTGGTCGAATTTCCA+AGG 0.558465 5_1:-58918288 Msa0740110:three_prime_UTR
AGAAGAGAAAGCAGAAACAA+AGG 0.563791 5_1:+58917892 Msa0740100:CDS
GACCAACCTTTCAGAACAGA+TGG 0.572272 5_1:+58917555 Msa0740100:CDS
GTATCATTCTCCTTCTGTGG+TGG 0.573843 5_1:-58918064 Msa0740110:three_prime_UTR
CTGGTAGCATGACAGTGAAA+CGG 0.576811 5_1:-58917240 None:intergenic
CATTCCTTCTGATTATGACA+CGG 0.581427 5_1:+58917601 Msa0740100:CDS
TGACCTCAAAACCTGCCCTA+AGG 0.581927 5_1:+58917519 Msa0740100:CDS
CGTGTCATAATCAGAAGGAA+TGG 0.584263 5_1:-58917600 None:intergenic
TAAAAGCAAAAGAGAAACAA+AGG 0.585363 5_1:+58917125 Msa0740100:five_prime_UTR
TTAACATGCAGGATATAGAA+GGG 0.585688 5_1:+58917478 Msa0740100:intron
CAAAACGTTGATGAACAAAA+GGG 0.585874 5_1:+58917854 Msa0740100:CDS
ATATAGAAGGGATCAAATGA+AGG 0.587427 5_1:+58917490 Msa0740100:CDS
AATACATTTGGATCGTCTCA+GGG 0.587906 5_1:+58918385 Msa0740100:CDS
TGAGCAAAAGGGTGCTCAAG+AGG 0.590093 5_1:+58917976 Msa0740100:CDS
ACCTGTGGTCGAATTTCCAA+GGG 0.591551 5_1:-58918287 Msa0740110:three_prime_UTR
TCAAAAGACACCACCACAGA+AGG 0.591734 5_1:+58918054 Msa0740100:CDS
CGACCACAGGTCTTAAGAAG+TGG 0.593414 5_1:+58918299 Msa0740100:CDS
ACAAAGTGGCGGAGAAAGTG+AGG 0.597287 5_1:+58918122 Msa0740100:CDS
GGTCTTGGAGCTTCTTCTGG+CGG 0.600665 5_1:-58917929 Msa0740110:three_prime_UTR
GAGCAAAAGGGTGCTCAAGA+GGG 0.603253 5_1:+58917977 Msa0740100:CDS
CTTCCACTTCTTAAGACCTG+TGG 0.604468 5_1:-58918302 Msa0740110:three_prime_UTR
ACGACGCCATCTGTTCTGAA+AGG 0.621474 5_1:-58917561 None:intergenic
AGAAGAAGCTCCAAGACCAA+AGG 0.632632 5_1:+58917934 Msa0740100:CDS
AGAAGCTAGCATAGACAAAG+TGG 0.640905 5_1:+58918108 Msa0740100:CDS
AGAAGCTGCTCCAAGACCAA+AGG 0.649599 5_1:+58917712 Msa0740100:CDS
CTGTCCGTGTCATAATCAGA+AGG 0.662264 5_1:-58917605 None:intergenic
AGCTAGCATAGACAAAGTGG+CGG 0.665250 5_1:+58918111 Msa0740100:CDS
TAATTGAAATGGTCATGACA+TGG 0.727826 5_1:-58917084 None:intergenic
GTGACTGCGACATTTGAGGG+TGG 0.759268 5_1:+58917629 Msa0740100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAAAAATGTATATTATT+TGG - chr5_1:58917326-58917345 None:intergenic 10.0%
!! GTAAAAAAATGTATATTATT+TGG - chr5_1:58917326-58917345 None:intergenic 10.0%
!!! TAATTCAAGTTTTTATATCT+TGG + chr5_1:58917271-58917290 Msa0740100:intron 15.0%
!! TCAAATTAATGAAAAACAAA+GGG - chr5_1:58917401-58917420 None:intergenic 15.0%
!! TTCAAATTAATGAAAAACAA+AGG - chr5_1:58917402-58917421 None:intergenic 15.0%
!!! TTTGTTTTTCATTAATTTGA+AGG + chr5_1:58917401-58917420 Msa0740100:intron 15.0%
!!! AGTATATAGTTTGTATTTTT+TGG - chr5_1:58917455-58917474 None:intergenic 15.0%
!!! TAATTCAAGTTTTTATATCT+TGG + chr5_1:58917271-58917290 Msa0740100:intron 15.0%
!! TCAAATTAATGAAAAACAAA+GGG - chr5_1:58917401-58917420 None:intergenic 15.0%
!! TTCAAATTAATGAAAAACAA+AGG - chr5_1:58917402-58917421 None:intergenic 15.0%
!!! TTTGTTTTTCATTAATTTGA+AGG + chr5_1:58917401-58917420 Msa0740100:intron 15.0%
!!! AGTATATAGTTTGTATTTTT+TGG - chr5_1:58917455-58917474 None:intergenic 15.0%
!! CTTTATTGTAGAAATACATT+TGG + chr5_1:58918373-58918392 Msa0740100:CDS 20.0%
!! CTTTATTGTAGAAATACATT+TGG + chr5_1:58918373-58918392 Msa0740100:CDS 20.0%
! TAAAAGCAAAAGAGAAACAA+AGG + chr5_1:58917125-58917144 Msa0740100:five_prime_UTR 25.0%
!! ACAAAGGCATAACTAATAAA+TGG + chr5_1:58917141-58917160 Msa0740100:exon 25.0%
! TATTTATCCCATTCATGATA+TGG - chr5_1:58917214-58917233 None:intergenic 25.0%
! AACTTGAATTAACATTTACC+TGG - chr5_1:58917262-58917281 None:intergenic 25.0%
! TAGTTGTCTTATGAGTTATT+TGG - chr5_1:58917361-58917380 None:intergenic 25.0%
!! AAACAAAGGGCTTTTAAATA+TGG - chr5_1:58917388-58917407 None:intergenic 25.0%
! TATATACTTTCTTAACATGC+AGG + chr5_1:58917467-58917486 Msa0740100:intron 25.0%
! TAAAAGCAAAAGAGAAACAA+AGG + chr5_1:58917125-58917144 Msa0740100:five_prime_UTR 25.0%
!! ACAAAGGCATAACTAATAAA+TGG + chr5_1:58917141-58917160 Msa0740100:exon 25.0%
! TATTTATCCCATTCATGATA+TGG - chr5_1:58917214-58917233 None:intergenic 25.0%
! AACTTGAATTAACATTTACC+TGG - chr5_1:58917262-58917281 None:intergenic 25.0%
! TAGTTGTCTTATGAGTTATT+TGG - chr5_1:58917361-58917380 None:intergenic 25.0%
!! AAACAAAGGGCTTTTAAATA+TGG - chr5_1:58917388-58917407 None:intergenic 25.0%
! TATATACTTTCTTAACATGC+AGG + chr5_1:58917467-58917486 Msa0740100:intron 25.0%
TAATTGAAATGGTCATGACA+TGG - chr5_1:58917087-58917106 None:intergenic 30.0%
TGACCATTTCAATTACTAAG+AGG + chr5_1:58917092-58917111 Msa0740100:five_prime_UTR 30.0%
TTGCCTCTTAGTAATTGAAA+TGG - chr5_1:58917098-58917117 None:intergenic 30.0%
ACTTTGAACCATATCATGAA+TGG + chr5_1:58917203-58917222 Msa0740100:CDS 30.0%
CTTTGAACCATATCATGAAT+GGG + chr5_1:58917204-58917223 Msa0740100:CDS 30.0%
CATGAATGGGATAAATATGA+TGG + chr5_1:58917217-58917236 Msa0740100:CDS 30.0%
TTAACATGCAGGATATAGAA+GGG + chr5_1:58917478-58917497 Msa0740100:intron 30.0%
ATATAGAAGGGATCAAATGA+AGG + chr5_1:58917490-58917509 Msa0740100:CDS 30.0%
TGCTAAACTTACTAATCCTA+AGG + chr5_1:58917670-58917689 Msa0740100:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_1:58917771-58917790 Msa0740110:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_1:58917772-58917791 Msa0740110:three_prime_UTR 30.0%
!!! ACTTTCTCTTCTTTAGCTTT+TGG - chr5_1:58917773-58917792 Msa0740110:three_prime_UTR 30.0%
AGAAGAGAAAGTAGAAACAA+AGG + chr5_1:58917781-58917800 Msa0740100:CDS 30.0%
! CAAAACGTTGATGAACAAAA+GGG + chr5_1:58917854-58917873 Msa0740100:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+AGG - chr5_1:58917771-58917790 Msa0740110:three_prime_UTR 30.0%
! TTTCTCTTCTATAGCTTTTG+GGG - chr5_1:58918005-58918024 Msa0740110:three_prime_UTR 30.0%
! CTTTCTCTTCTATAGCTTTT+GGG - chr5_1:58918006-58918025 Msa0740110:three_prime_UTR 30.0%
TTTGCATCTAGCAATAAACT+TGG - chr5_1:58918209-58918228 Msa0740110:three_prime_UTR 30.0%
ATGTGTCATGCTAATCTTAT+TGG + chr5_1:58918336-58918355 Msa0740100:CDS 30.0%
TAATTGAAATGGTCATGACA+TGG - chr5_1:58917087-58917106 None:intergenic 30.0%
TGACCATTTCAATTACTAAG+AGG + chr5_1:58917092-58917111 Msa0740100:five_prime_UTR 30.0%
TTGCCTCTTAGTAATTGAAA+TGG - chr5_1:58917098-58917117 None:intergenic 30.0%
ACTTTGAACCATATCATGAA+TGG + chr5_1:58917203-58917222 Msa0740100:CDS 30.0%
CTTTGAACCATATCATGAAT+GGG + chr5_1:58917204-58917223 Msa0740100:CDS 30.0%
CATGAATGGGATAAATATGA+TGG + chr5_1:58917217-58917236 Msa0740100:CDS 30.0%
TTAACATGCAGGATATAGAA+GGG + chr5_1:58917478-58917497 Msa0740100:intron 30.0%
ATATAGAAGGGATCAAATGA+AGG + chr5_1:58917490-58917509 Msa0740100:CDS 30.0%
TGCTAAACTTACTAATCCTA+AGG + chr5_1:58917670-58917689 Msa0740100:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_1:58917771-58917790 Msa0740110:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_1:58917772-58917791 Msa0740110:three_prime_UTR 30.0%
!!! ACTTTCTCTTCTTTAGCTTT+TGG - chr5_1:58917773-58917792 Msa0740110:three_prime_UTR 30.0%
AGAAGAGAAAGTAGAAACAA+AGG + chr5_1:58917781-58917800 Msa0740100:CDS 30.0%
! CAAAACGTTGATGAACAAAA+GGG + chr5_1:58917854-58917873 Msa0740100:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+AGG - chr5_1:58917771-58917790 Msa0740110:three_prime_UTR 30.0%
! TTTCTCTTCTATAGCTTTTG+GGG - chr5_1:58918005-58918024 Msa0740110:three_prime_UTR 30.0%
! CTTTCTCTTCTATAGCTTTT+GGG - chr5_1:58918006-58918025 Msa0740110:three_prime_UTR 30.0%
TTTGCATCTAGCAATAAACT+TGG - chr5_1:58918209-58918228 Msa0740110:three_prime_UTR 30.0%
ATGTGTCATGCTAATCTTAT+TGG + chr5_1:58918336-58918355 Msa0740100:CDS 30.0%
CTTAACATGCAGGATATAGA+AGG + chr5_1:58917477-58917496 Msa0740100:intron 35.0%
GTGTCATAATCAGAAGGAAT+GGG - chr5_1:58917602-58917621 None:intergenic 35.0%
CATTCCTTCTGATTATGACA+CGG + chr5_1:58917601-58917620 Msa0740100:CDS 35.0%
!! GGATTTGTTGTTGTTTCCTT+AGG - chr5_1:58917689-58917708 Msa0740110:three_prime_UTR 35.0%
!! ACAAAAGGTTGATGAGCAAA+AGG + chr5_1:58917742-58917761 Msa0740100:CDS 35.0%
!! CAAAAGGTTGATGAGCAAAA+GGG + chr5_1:58917743-58917762 Msa0740100:CDS 35.0%
! GCAAAACGTTGATGAACAAA+AGG + chr5_1:58917853-58917872 Msa0740100:CDS 35.0%
AGAAGAGAAAGCAGAAACAA+AGG + chr5_1:58917892-58917911 Msa0740100:CDS 35.0%
! GCTTTCTCTTCTATAGCTTT+TGG - chr5_1:58918007-58918026 Msa0740110:three_prime_UTR 35.0%
ATTGTATCATTCTCCTTCTG+TGG - chr5_1:58918070-58918089 Msa0740110:three_prime_UTR 35.0%
!!! AACTTGGCATCTTTGGTTTT+AGG - chr5_1:58918193-58918212 Msa0740110:three_prime_UTR 35.0%
AGCAATAAACTTGGCATCTT+TGG - chr5_1:58918200-58918219 Msa0740110:three_prime_UTR 35.0%
TTATTGCTAGATGCAAAACC+AGG + chr5_1:58918212-58918231 Msa0740100:CDS 35.0%
TGTAGATTTCACTCTCTCTA+AGG + chr5_1:58918237-58918256 Msa0740100:CDS 35.0%
TCTTAAGAAGTGGAAGAGAT+TGG + chr5_1:58918309-58918328 Msa0740100:CDS 35.0%
ATGCTAATCTTATTGGTCGT+GGG + chr5_1:58918343-58918362 Msa0740100:CDS 35.0%
AAATACATTTGGATCGTCTC+AGG + chr5_1:58918384-58918403 Msa0740100:CDS 35.0%
AATACATTTGGATCGTCTCA+GGG + chr5_1:58918385-58918404 Msa0740100:CDS 35.0%
CTTAACATGCAGGATATAGA+AGG + chr5_1:58917477-58917496 Msa0740100:intron 35.0%
GTGTCATAATCAGAAGGAAT+GGG - chr5_1:58917602-58917621 None:intergenic 35.0%
CATTCCTTCTGATTATGACA+CGG + chr5_1:58917601-58917620 Msa0740100:CDS 35.0%
!! GGATTTGTTGTTGTTTCCTT+AGG - chr5_1:58917689-58917708 Msa0740110:three_prime_UTR 35.0%
!! ACAAAAGGTTGATGAGCAAA+AGG + chr5_1:58917742-58917761 Msa0740100:CDS 35.0%
!! CAAAAGGTTGATGAGCAAAA+GGG + chr5_1:58917743-58917762 Msa0740100:CDS 35.0%
! GCAAAACGTTGATGAACAAA+AGG + chr5_1:58917853-58917872 Msa0740100:CDS 35.0%
AGAAGAGAAAGCAGAAACAA+AGG + chr5_1:58917892-58917911 Msa0740100:CDS 35.0%
! GCTTTCTCTTCTATAGCTTT+TGG - chr5_1:58918007-58918026 Msa0740110:three_prime_UTR 35.0%
ATTGTATCATTCTCCTTCTG+TGG - chr5_1:58918070-58918089 Msa0740110:three_prime_UTR 35.0%
!!! AACTTGGCATCTTTGGTTTT+AGG - chr5_1:58918193-58918212 Msa0740110:three_prime_UTR 35.0%
AGCAATAAACTTGGCATCTT+TGG - chr5_1:58918200-58918219 Msa0740110:three_prime_UTR 35.0%
TTATTGCTAGATGCAAAACC+AGG + chr5_1:58918212-58918231 Msa0740100:CDS 35.0%
TGTAGATTTCACTCTCTCTA+AGG + chr5_1:58918237-58918256 Msa0740100:CDS 35.0%
TCTTAAGAAGTGGAAGAGAT+TGG + chr5_1:58918309-58918328 Msa0740100:CDS 35.0%
ATGCTAATCTTATTGGTCGT+GGG + chr5_1:58918343-58918362 Msa0740100:CDS 35.0%
AAATACATTTGGATCGTCTC+AGG + chr5_1:58918384-58918403 Msa0740100:CDS 35.0%
AATACATTTGGATCGTCTCA+GGG + chr5_1:58918385-58918404 Msa0740100:CDS 35.0%
TCTTTCACCTATCAGCCTTA+GGG - chr5_1:58917537-58917556 None:intergenic 40.0%
CGTGTCATAATCAGAAGGAA+TGG - chr5_1:58917603-58917622 None:intergenic 40.0%
ACCAAAGGAACCATCACAAA+AGG + chr5_1:58917727-58917746 Msa0740100:CDS 40.0%
! ACCTTTTGTGATGGTTCCTT+TGG - chr5_1:58917731-58917750 Msa0740110:three_prime_UTR 40.0%
! TGCTCATCAACCTTTTGTGA+TGG - chr5_1:58917740-58917759 Msa0740110:three_prime_UTR 40.0%
!! TTTAGTCTTGGAGCTTCTTC+TGG - chr5_1:58917824-58917843 Msa0740110:three_prime_UTR 40.0%
AGAAGAAGCTCCAAGACTAA+AGG + chr5_1:58917823-58917842 Msa0740100:CDS 40.0%
! TGTTCATCAACGTTTTGCGA+TGG - chr5_1:58917851-58917870 Msa0740110:three_prime_UTR 40.0%
! TTTTGCTCATCAGCCTTTTG+TGG - chr5_1:58917965-58917984 Msa0740110:three_prime_UTR 40.0%
ACAAAAGGCTGATGAGCAAA+AGG + chr5_1:58917964-58917983 Msa0740100:CDS 40.0%
CAAAAGGCTGATGAGCAAAA+GGG + chr5_1:58917965-58917984 Msa0740100:CDS 40.0%
! AGCTTCTGTTCTGCTCTTTT+CGG - chr5_1:58918095-58918114 Msa0740110:three_prime_UTR 40.0%
AGAAGCTAGCATAGACAAAG+TGG + chr5_1:58918108-58918127 Msa0740100:CDS 40.0%
CATGCTAATCTTATTGGTCG+TGG + chr5_1:58918342-58918361 Msa0740100:CDS 40.0%
TCTTTCACCTATCAGCCTTA+GGG - chr5_1:58917537-58917556 None:intergenic 40.0%
CGTGTCATAATCAGAAGGAA+TGG - chr5_1:58917603-58917622 None:intergenic 40.0%
ACCAAAGGAACCATCACAAA+AGG + chr5_1:58917727-58917746 Msa0740100:CDS 40.0%
! ACCTTTTGTGATGGTTCCTT+TGG - chr5_1:58917731-58917750 Msa0740110:three_prime_UTR 40.0%
! TGCTCATCAACCTTTTGTGA+TGG - chr5_1:58917740-58917759 Msa0740110:three_prime_UTR 40.0%
!! TTTAGTCTTGGAGCTTCTTC+TGG - chr5_1:58917824-58917843 Msa0740110:three_prime_UTR 40.0%
AGAAGAAGCTCCAAGACTAA+AGG + chr5_1:58917823-58917842 Msa0740100:CDS 40.0%
! TGTTCATCAACGTTTTGCGA+TGG - chr5_1:58917851-58917870 Msa0740110:three_prime_UTR 40.0%
! TTTTGCTCATCAGCCTTTTG+TGG - chr5_1:58917965-58917984 Msa0740110:three_prime_UTR 40.0%
ACAAAAGGCTGATGAGCAAA+AGG + chr5_1:58917964-58917983 Msa0740100:CDS 40.0%
CAAAAGGCTGATGAGCAAAA+GGG + chr5_1:58917965-58917984 Msa0740100:CDS 40.0%
! AGCTTCTGTTCTGCTCTTTT+CGG - chr5_1:58918095-58918114 Msa0740110:three_prime_UTR 40.0%
AGAAGCTAGCATAGACAAAG+TGG + chr5_1:58918108-58918127 Msa0740100:CDS 40.0%
CATGCTAATCTTATTGGTCG+TGG + chr5_1:58918342-58918361 Msa0740100:CDS 40.0%
TCATAGACACGATCAGCTTC+TGG - chr5_1:58917181-58917200 None:intergenic 45.0%
CTGGTAGCATGACAGTGAAA+CGG - chr5_1:58917243-58917262 None:intergenic 45.0%
GTCTTTCACCTATCAGCCTT+AGG - chr5_1:58917538-58917557 None:intergenic 45.0%
GACCAACCTTTCAGAACAGA+TGG + chr5_1:58917555-58917574 Msa0740100:CDS 45.0%
CTGTCCGTGTCATAATCAGA+AGG - chr5_1:58917608-58917627 None:intergenic 45.0%
AGTGTGACTGCGACATTTGA+GGG + chr5_1:58917626-58917645 Msa0740100:CDS 45.0%
TGTGATGGTTCCTTTGGTCT+TGG - chr5_1:58917725-58917744 Msa0740110:three_prime_UTR 45.0%
TGCGATGGTTCCTTTAGTCT+TGG - chr5_1:58917836-58917855 Msa0740110:three_prime_UTR 45.0%
!! TTTGGTCTTGGAGCTTCTTC+TGG - chr5_1:58917935-58917954 Msa0740110:three_prime_UTR 45.0%
AGAAGAAGCTCCAAGACCAA+AGG + chr5_1:58917934-58917953 Msa0740100:CDS 45.0%
ACCAAAGGAATCGCCACAAA+AGG + chr5_1:58917949-58917968 Msa0740100:CDS 45.0%
TCAAAAGACACCACCACAGA+AGG + chr5_1:58918054-58918073 Msa0740100:CDS 45.0%
GTATCATTCTCCTTCTGTGG+TGG - chr5_1:58918067-58918086 Msa0740110:three_prime_UTR 45.0%
AGCTAGCATAGACAAAGTGG+CGG + chr5_1:58918111-58918130 Msa0740100:CDS 45.0%
GAGAGTGAAATCTACAAGCC+TGG - chr5_1:58918233-58918252 Msa0740110:three_prime_UTR 45.0%
!! CTCTAAGGCTCTCTGTAATC+AGG + chr5_1:58918252-58918271 Msa0740100:CDS 45.0%
CAGGATGAAAATGAAGCCCT+TGG + chr5_1:58918271-58918290 Msa0740100:CDS 45.0%
ACCTGTGGTCGAATTTCCAA+GGG - chr5_1:58918290-58918309 Msa0740110:three_prime_UTR 45.0%
CTTCCACTTCTTAAGACCTG+TGG - chr5_1:58918305-58918324 Msa0740110:three_prime_UTR 45.0%
ATCTTATTGGTCGTGGGACT+TGG + chr5_1:58918349-58918368 Msa0740100:CDS 45.0%
TCTTATTGGTCGTGGGACTT+GGG + chr5_1:58918350-58918369 Msa0740100:CDS 45.0%
TCATAGACACGATCAGCTTC+TGG - chr5_1:58917181-58917200 None:intergenic 45.0%
CTGGTAGCATGACAGTGAAA+CGG - chr5_1:58917243-58917262 None:intergenic 45.0%
GTCTTTCACCTATCAGCCTT+AGG - chr5_1:58917538-58917557 None:intergenic 45.0%
GACCAACCTTTCAGAACAGA+TGG + chr5_1:58917555-58917574 Msa0740100:CDS 45.0%
CTGTCCGTGTCATAATCAGA+AGG - chr5_1:58917608-58917627 None:intergenic 45.0%
AGTGTGACTGCGACATTTGA+GGG + chr5_1:58917626-58917645 Msa0740100:CDS 45.0%
TGTGATGGTTCCTTTGGTCT+TGG - chr5_1:58917725-58917744 Msa0740110:three_prime_UTR 45.0%
TGCGATGGTTCCTTTAGTCT+TGG - chr5_1:58917836-58917855 Msa0740110:three_prime_UTR 45.0%
!! TTTGGTCTTGGAGCTTCTTC+TGG - chr5_1:58917935-58917954 Msa0740110:three_prime_UTR 45.0%
AGAAGAAGCTCCAAGACCAA+AGG + chr5_1:58917934-58917953 Msa0740100:CDS 45.0%
ACCAAAGGAATCGCCACAAA+AGG + chr5_1:58917949-58917968 Msa0740100:CDS 45.0%
TCAAAAGACACCACCACAGA+AGG + chr5_1:58918054-58918073 Msa0740100:CDS 45.0%
GTATCATTCTCCTTCTGTGG+TGG - chr5_1:58918067-58918086 Msa0740110:three_prime_UTR 45.0%
AGCTAGCATAGACAAAGTGG+CGG + chr5_1:58918111-58918130 Msa0740100:CDS 45.0%
GAGAGTGAAATCTACAAGCC+TGG - chr5_1:58918233-58918252 Msa0740110:three_prime_UTR 45.0%
!! CTCTAAGGCTCTCTGTAATC+AGG + chr5_1:58918252-58918271 Msa0740100:CDS 45.0%
CAGGATGAAAATGAAGCCCT+TGG + chr5_1:58918271-58918290 Msa0740100:CDS 45.0%
ACCTGTGGTCGAATTTCCAA+GGG - chr5_1:58918290-58918309 Msa0740110:three_prime_UTR 45.0%
CTTCCACTTCTTAAGACCTG+TGG - chr5_1:58918305-58918324 Msa0740110:three_prime_UTR 45.0%
ATCTTATTGGTCGTGGGACT+TGG + chr5_1:58918349-58918368 Msa0740100:CDS 45.0%
TCTTATTGGTCGTGGGACTT+GGG + chr5_1:58918350-58918369 Msa0740100:CDS 45.0%
!! TAAAAAAATGTATATTATTT+GGG - chr5_1:58917325-58917344 None:intergenic 5.0%
!! TAAAAAAATGTATATTATTT+GGG - chr5_1:58917325-58917344 None:intergenic 5.0%
CGTTTCACTGTCATGCTACC+AGG + chr5_1:58917241-58917260 Msa0740100:CDS 50.0%
TGACCTCAAAACCTGCCCTA+AGG + chr5_1:58917519-58917538 Msa0740100:CDS 50.0%
AAACCTGCCCTAAGGCTGAT+AGG + chr5_1:58917527-58917546 Msa0740100:CDS 50.0%
!! CGCCATCTGTTCTGAAAGGT+TGG - chr5_1:58917560-58917579 None:intergenic 50.0%
! ACGACGCCATCTGTTCTGAA+AGG - chr5_1:58917564-58917583 None:intergenic 50.0%
CAGTGTGACTGCGACATTTG+AGG + chr5_1:58917625-58917644 Msa0740100:CDS 50.0%
!! TTTGGTCTTGGAGCAGCTTC+TGG - chr5_1:58917713-58917732 Msa0740110:three_prime_UTR 50.0%
AGAAGCTGCTCCAAGACCAA+AGG + chr5_1:58917712-58917731 Msa0740100:CDS 50.0%
AGTCTTGGAGCTTCTTCTGG+TGG - chr5_1:58917821-58917840 Msa0740110:three_prime_UTR 50.0%
TGTGGCGATTCCTTTGGTCT+TGG - chr5_1:58917947-58917966 Msa0740110:three_prime_UTR 50.0%
! GCCTTTTGTGGCGATTCCTT+TGG - chr5_1:58917953-58917972 Msa0740110:three_prime_UTR 50.0%
!! TGAGCAAAAGGGTGCTCAAG+AGG + chr5_1:58917976-58917995 Msa0740100:CDS 50.0%
!! GAGCAAAAGGGTGCTCAAGA+GGG + chr5_1:58917977-58917996 Msa0740100:CDS 50.0%
! ACAAAGTGGCGGAGAAAGTG+AGG + chr5_1:58918122-58918141 Msa0740100:CDS 50.0%
GCGGAGAAAGTGAGGACTAA+TGG + chr5_1:58918130-58918149 Msa0740100:CDS 50.0%
GACCTGTGGTCGAATTTCCA+AGG - chr5_1:58918291-58918310 Msa0740110:three_prime_UTR 50.0%
CGACCACAGGTCTTAAGAAG+TGG + chr5_1:58918299-58918318 Msa0740100:CDS 50.0%
TGGATCGTCTCAGGGAGAAT+TGG + chr5_1:58918393-58918412 Msa0740100:CDS 50.0%
CGTTTCACTGTCATGCTACC+AGG + chr5_1:58917241-58917260 Msa0740100:CDS 50.0%
TGACCTCAAAACCTGCCCTA+AGG + chr5_1:58917519-58917538 Msa0740100:CDS 50.0%
AAACCTGCCCTAAGGCTGAT+AGG + chr5_1:58917527-58917546 Msa0740100:CDS 50.0%
!! CGCCATCTGTTCTGAAAGGT+TGG - chr5_1:58917560-58917579 None:intergenic 50.0%
! ACGACGCCATCTGTTCTGAA+AGG - chr5_1:58917564-58917583 None:intergenic 50.0%
CAGTGTGACTGCGACATTTG+AGG + chr5_1:58917625-58917644 Msa0740100:CDS 50.0%
!! TTTGGTCTTGGAGCAGCTTC+TGG - chr5_1:58917713-58917732 Msa0740110:three_prime_UTR 50.0%
AGAAGCTGCTCCAAGACCAA+AGG + chr5_1:58917712-58917731 Msa0740100:CDS 50.0%
AGTCTTGGAGCTTCTTCTGG+TGG - chr5_1:58917821-58917840 Msa0740110:three_prime_UTR 50.0%
TGTGGCGATTCCTTTGGTCT+TGG - chr5_1:58917947-58917966 Msa0740110:three_prime_UTR 50.0%
! GCCTTTTGTGGCGATTCCTT+TGG - chr5_1:58917953-58917972 Msa0740110:three_prime_UTR 50.0%
!! TGAGCAAAAGGGTGCTCAAG+AGG + chr5_1:58917976-58917995 Msa0740100:CDS 50.0%
!! GAGCAAAAGGGTGCTCAAGA+GGG + chr5_1:58917977-58917996 Msa0740100:CDS 50.0%
! ACAAAGTGGCGGAGAAAGTG+AGG + chr5_1:58918122-58918141 Msa0740100:CDS 50.0%
GCGGAGAAAGTGAGGACTAA+TGG + chr5_1:58918130-58918149 Msa0740100:CDS 50.0%
GACCTGTGGTCGAATTTCCA+AGG - chr5_1:58918291-58918310 Msa0740110:three_prime_UTR 50.0%
CGACCACAGGTCTTAAGAAG+TGG + chr5_1:58918299-58918318 Msa0740100:CDS 50.0%
TGGATCGTCTCAGGGAGAAT+TGG + chr5_1:58918393-58918412 Msa0740100:CDS 50.0%
! CAGCCTTAGGGCAGGTTTTG+AGG - chr5_1:58917525-58917544 None:intergenic 55.0%
TCACCTATCAGCCTTAGGGC+AGG - chr5_1:58917533-58917552 None:intergenic 55.0%
GTGACTGCGACATTTGAGGG+TGG + chr5_1:58917629-58917648 Msa0740100:CDS 55.0%
GGTCTTGGAGCTTCTTCTGG+CGG - chr5_1:58917932-58917951 Msa0740110:three_prime_UTR 55.0%
GCCCTTGGAAATTCGACCAC+AGG + chr5_1:58918286-58918305 Msa0740100:CDS 55.0%
! CAGCCTTAGGGCAGGTTTTG+AGG - chr5_1:58917525-58917544 None:intergenic 55.0%
TCACCTATCAGCCTTAGGGC+AGG - chr5_1:58917533-58917552 None:intergenic 55.0%
GTGACTGCGACATTTGAGGG+TGG + chr5_1:58917629-58917648 Msa0740100:CDS 55.0%
GGTCTTGGAGCTTCTTCTGG+CGG - chr5_1:58917932-58917951 Msa0740110:three_prime_UTR 55.0%
GCCCTTGGAAATTCGACCAC+AGG + chr5_1:58918286-58918305 Msa0740100:CDS 55.0%
GGTCTTGGAGCAGCTTCTGG+TGG - chr5_1:58917710-58917729 Msa0740110:three_prime_UTR 60.0%
GGTCTTGGAGCAGCTTCTGG+TGG - chr5_1:58917710-58917729 Msa0740110:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 58917017 58918447 58917017 ID=Msa0740100;Name=Msa0740100
chr5_1 mRNA 58917017 58918447 58917017 ID=Msa0740100-mRNA-1;Parent=Msa0740100;Name=Msa0740100-mRNA-1;_AED=0.24;_eAED=0.24;_QI=143|1|0.5|1|1|1|2|0|353
chr5_1 exon 58917017 58917262 58917017 ID=Msa0740100-mRNA-1:exon:16869;Parent=Msa0740100-mRNA-1
chr5_1 exon 58917489 58918447 58917489 ID=Msa0740100-mRNA-1:exon:16870;Parent=Msa0740100-mRNA-1
chr5_1 five_prime_UTR 58917017 58917159 58917017 ID=Msa0740100-mRNA-1:five_prime_utr;Parent=Msa0740100-mRNA-1
chr5_1 CDS 58917160 58917262 58917160 ID=Msa0740100-mRNA-1:cds;Parent=Msa0740100-mRNA-1
chr5_1 CDS 58917489 58918447 58917489 ID=Msa0740100-mRNA-1:cds;Parent=Msa0740100-mRNA-1
Gene Sequence

>Msa0740100

ATGGATACAAAGACACAGCCAGAAGCTGATCGTGTCTATGAAGACTTTGAACCATATCATGAATGGGATAAATATGATGGCCGTTTCACTGTCATGCTACCAGGATATAGAAGGGATCAAATGAAGGTTCAAGTGACCTCAAAACCTGCCCTAAGGCTGATAGGTGAAAGACCAACCTTTCAGAACAGATGGCGTCGTTTTAAATTAGAATTTCCCATTCCTTCTGATTATGACACGGACAGTGTGACTGCGACATTTGAGGGTGGCAAGCTATCTGTCAAATTTGCTAAACTTACTAATCCTAAGGAAACAACAACAAATCCACCAGAAGCTGCTCCAAGACCAAAGGAACCATCACAAAAGGTTGATGAGCAAAAGGGTACCCCAAAAGCTAAAGAAGAGAAAGTAGAAACAAAGGAAACAACAACAAATCCACCAGAAGAAGCTCCAAGACTAAAGGAACCATCGCAAAACGTTGATGAACAAAAGGGTACCTCAAAAGCTAAAGAAGAGAAAGCAGAAACAAAGGAAACAACAACAAATCCGCCAGAAGAAGCTCCAAGACCAAAGGAATCGCCACAAAAGGCTGATGAGCAAAAGGGTGCTCAAGAGGGTACCCCAAAAGCTATAGAAGAGAAAGCAGAAACAAAGACTAACGATGTCTCTGATCAAAAGACACCACCACAGAAGGAGAATGATACAATAACCGAAAAGAGCAGAACAGAAGCTAGCATAGACAAAGTGGCGGAGAAAGTGAGGACTAATGGCTCAGCTGAGACAATAGACGCAGCAACATCTAATTCTCCTAAAACCAAAGATGCCAAGTTTATTGCTAGATGCAAAACCAGGCTTGTAGATTTCACTCTCTCTAAGGCTCTCTGTAATCAGGATGAAAATGAAGCCCTTGGAAATTCGACCACAGGTCTTAAGAAGTGGAAGAGATTGGTTGCATGTGTCATGCTAATCTTATTGGTCGTGGGACTTGGGCTTTATTGTAGAAATACATTTGGATCGTCTCAGGGAGAATTGGACTTCGAAGAGCTGTTATTATTTCCTTACTGA
Protein sequence

>Msa0740100

MDTKTQPEADRVYEDFEPYHEWDKYDGRFTVMLPGYRRDQMKVQVTSKPALRLIGERPTFQNRWRRFKLEFPIPSDYDTDSVTATFEGGKLSVKFAKLTNPKETTTNPPEAAPRPKEPSQKVDEQKGTPKAKEEKVETKETTTNPPEEAPRLKEPSQNVDEQKGTSKAKEEKAETKETTTNPPEEAPRPKESPQKADEQKGAQEGTPKAIEEKAETKTNDVSDQKTPPQKENDTITEKSRTEASIDKVAEKVRTNGSAETIDAATSNSPKTKDAKFIARCKTRLVDFTLSKALCNQDENEALGNSTTGLKKWKRLVACVMLILLVVGLGLYCRNTFGSSQGELDFEELLLFPY*