AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0414250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414250 MtrunA17_Chr3g0125061 86.246 618 77 2 219 836 75 684 0.0 1101
Msa0414250 MtrunA17_Chr2g0304131 95.495 222 10 0 68 289 45 266 4.44e-145 430
Msa0414250 MtrunA17_Chr1g0161141 48.295 528 237 14 296 791 121 644 2.06e-144 442
Msa0414250 MtrunA17_Chr1g0161151 46.604 530 246 14 294 791 89 613 1.66e-141 433
Msa0414250 MtrunA17_Chr3g0096771 74.528 212 31 2 83 294 57 245 9.95e-93 293
Msa0414250 MtrunA17_Chr3g0107261 67.421 221 67 1 74 294 140 355 5.80e-88 284
Msa0414250 MtrunA17_Chr3g0105591 46.526 331 123 5 11 295 70 392 1.12e-71 242
Msa0414250 MtrunA17_Chr3g0105471 61.395 215 75 3 80 294 3 209 2.01e-68 226
Msa0414250 MtrunA17_Chr3g0108171 50.333 300 115 5 11 294 1642 1923 8.66e-68 246
Msa0414250 MtrunA17_Chr3g0108171 37.821 156 64 5 10 140 1449 1596 1.15e-13 75.5
Msa0414250 MtrunA17_Chr3g0108821 52.113 284 107 6 11 294 1504 1758 2.11e-67 244
Msa0414250 MtrunA17_Chr3g0087641 45.763 295 115 4 20 281 64 346 6.79e-66 223
Msa0414250 MtrunA17_Chr3g0105551 45.210 334 124 5 12 294 1495 1820 1.34e-63 233
Msa0414250 MtrunA17_Chr3g0097011 50.929 269 91 6 47 287 105 360 4.14e-63 218
Msa0414250 MtrunA17_Chr3g0108151 48.070 285 113 6 22 295 1247 1507 1.44e-60 224
Msa0414250 MtrunA17_Chr3g0105521 51.894 264 99 5 47 294 1317 1568 1.41e-59 221
Msa0414250 MtrunA17_Chr3g0108091 61.702 188 65 2 101 288 312 492 1.42e-59 211
Msa0414250 MtrunA17_Chr3g0108811 47.887 284 110 7 11 294 1648 1893 1.63e-57 214
Msa0414250 MtrunA17_Chr3g0108071 61.702 188 65 2 101 288 775 955 4.73e-57 211
Msa0414250 MtrunA17_Chr3g0108761 69.784 139 42 0 150 288 6 144 2.40e-56 191
Msa0414250 MtrunA17_Chr3g0107171 84.034 119 14 2 177 294 225 339 7.18e-54 191
Msa0414250 MtrunA17_Chr3g0107171 78.462 65 13 1 47 111 167 230 2.79e-21 97.1
Msa0414250 MtrunA17_Chr3g0091861 39.211 380 110 7 11 288 1477 1837 2.05e-53 202
Msa0414250 MtrunA17_Chr3g0107931 44.231 312 101 6 24 288 1552 1837 3.64e-53 201
Msa0414250 MtrunA17_Chr3g0107641 66.897 145 47 1 150 294 5 148 3.31e-52 180
Msa0414250 MtrunA17_Chr3g0091851 54.902 204 79 3 85 288 1089 1279 2.69e-50 192
Msa0414250 MtrunA17_Chr7g0223911 71.579 95 27 0 201 295 30 124 1.21e-39 144
Msa0414250 MtrunA17_Chr7g0229711 53.333 120 47 2 169 288 5 115 1.81e-31 120
Msa0414250 MtrunA17_Chr7g0229731 82.258 62 11 0 83 144 38 99 4.26e-26 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414250 AT1G21680.1 70.501 539 147 6 294 822 161 697 0.0 791
Msa0414250 AT1G21670.1 59.398 532 203 6 294 814 147 676 0.0 642
Msa0414250 AT4G01870.2 39.626 641 336 17 190 791 38 666 1.20e-136 422
Msa0414250 AT4G01870.1 43.214 560 277 14 270 791 61 617 1.62e-136 421

Find 217 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACAGGCTTGATATATT+TGG 0.102269 3_2:-59316692 None:intergenic
ACTGGTCTCCGAGAATTATT+TGG 0.203015 3_2:+59314723 Msa0414250:CDS
ACGCAACACTTCAACTTCTT+TGG 0.203982 3_2:-59315064 None:intergenic
CACCATCGCCAGACGGAAAA+TGG 0.208864 3_2:+59316218 Msa0414250:CDS
TCATAAGAATCTTTACTTAA+TGG 0.226205 3_2:+59316267 Msa0414250:CDS
TCATATTTCTTTCTTGTTAG+AGG 0.241231 3_2:+59313534 Msa0414250:CDS
CTGTATCTCCTACCGGAAAA+TGG 0.260326 3_2:+59315363 Msa0414250:CDS
TTTCCATCGGGGCTAAAATA+TGG 0.281247 3_2:-59316505 None:intergenic
ATCTCAAAGCTTCCACTTCC+CGG 0.305361 3_2:-59316409 None:intergenic
GTTCTCCTTGTATTGTCATA+TGG 0.314540 3_2:+59314689 Msa0414250:CDS
TTCACAGGCTTGATATATTT+GGG 0.319302 3_2:-59316691 None:intergenic
TCAACTTCTTGACGAAATTT+CGG 0.322692 3_2:-59316165 None:intergenic
CGCCGTAGGGAGTGAGTCTT+CGG 0.333993 3_2:-59315322 None:intergenic
TTCCTTAACCCGGTTCCATT+CGG 0.340230 3_2:-59316445 None:intergenic
GCGATGAATTTGTTGTTTCC+AGG 0.349537 3_2:-59315671 None:intergenic
CCGCATCATTATCAACCTTA+TGG 0.372423 3_2:+59316583 Msa0414250:CDS
TTCCAAATCTAACTCTCTTC+CGG 0.374065 3_2:+59315912 Msa0414250:CDS
AAGGAAACGAACCGGTGAAC+CGG 0.377127 3_2:-59315931 None:intergenic
TTGATCCACCCGAATGGAAC+CGG 0.378646 3_2:+59316436 Msa0414250:CDS
TCCTTAACCCGGTTCCATTC+GGG 0.381201 3_2:-59316444 None:intergenic
CTTTCTCAACCGCATGGGAC+CGG 0.387357 3_2:+59316056 Msa0414250:CDS
CTAACACATCCAGCACACTA+TGG 0.390192 3_2:-59314882 None:intergenic
ACCCGAATGGAACCGGGTTA+AGG 0.392765 3_2:+59316443 Msa0414250:CDS
TTGTTTCCAGGTGAAGTTGC+CGG 0.392945 3_2:-59315659 None:intergenic
TACCCGACCCGCGTTGAATC+AGG 0.394302 3_2:-59315821 None:intergenic
CTACACAATTTGCGCAGCTT+TGG 0.398392 3_2:+59314934 Msa0414250:CDS
AACCGGAAGAGAGTTAGATT+TGG 0.399322 3_2:-59315914 None:intergenic
ACCCTCAGCTCATTTAGAAT+TGG 0.400240 3_2:+59314049 Msa0414250:CDS
AAACTGATTCAAAGTGGTTC+AGG 0.400842 3_2:+59316466 Msa0414250:CDS
AGCACGGTGGCTGGCCAAGT+TGG 0.401342 3_2:+59315498 Msa0414250:CDS
AGGCATTGTTCTTTCCGTCT+AGG 0.409708 3_2:-59316189 None:intergenic
CATATGGGAGCTTGAGCAAC+TGG 0.410646 3_2:+59314705 Msa0414250:CDS
CTCGTAATCCAAATAATTCT+CGG 0.413416 3_2:-59314731 None:intergenic
TTCTCCTTGTATTGTCATAT+GGG 0.414016 3_2:+59314690 Msa0414250:CDS
ATTAAACCCTTCTGAAATAG+TGG 0.415341 3_2:+59314657 Msa0414250:CDS
TTGAATCAGGCGAGATAAAC+GGG 0.418237 3_2:-59315808 None:intergenic
TATTCCACCCATCTCAAATC+TGG 0.421577 3_2:+59315293 Msa0414250:CDS
GTTGAATCAGGCGAGATAAA+CGG 0.424517 3_2:-59315809 None:intergenic
CCTGTACTAACAATGTAGTT+TGG 0.430014 3_2:-59316778 None:intergenic
ACTCATAATTCTTATGAAGA+TGG 0.434923 3_2:+59316652 Msa0414250:CDS
TACTTAATGGATGCCGTTCA+CGG 0.435170 3_2:+59316280 Msa0414250:CDS
CTGGTGTTTACGTAGTGAAC+CGG 0.435407 3_2:+59315996 Msa0414250:CDS
CAATCCTCAAATGAACAATA+AGG 0.437311 3_2:-59316736 None:intergenic
TTTACACCGGCAACTTCACC+TGG 0.437505 3_2:+59315653 Msa0414250:CDS
AAAGCGATCCATTCTCCATC+AGG 0.440483 3_2:-59316367 None:intergenic
TCAATTAAGCTCAATTATTG+AGG 0.441514 3_2:+59314439 Msa0414250:CDS
GCACGGTGGCTGGCCAAGTT+GGG 0.445351 3_2:+59315499 Msa0414250:CDS
ACCGTGAGGTTGGATGGTTC+AGG 0.446696 3_2:+59316616 Msa0414250:CDS
AAGAGAGTTAGATTTGGAAC+GGG 0.447749 3_2:-59315908 None:intergenic
GGCATTGTTCTTTCCGTCTA+GGG 0.447828 3_2:-59316188 None:intergenic
GATCGTCATGACCCGGGAAG+TGG 0.448872 3_2:+59316397 Msa0414250:CDS
CATCCAGCACACTATGGGTT+TGG 0.451580 3_2:-59314876 None:intergenic
ACTATATTACATTATTTCCA+TGG 0.453379 3_2:-59313645 None:intergenic
ACATTGGTCATTATTAGTCT+TGG 0.453833 3_2:-59314305 None:intergenic
CTCAGTGCTTTCTGAAGCAA+AGG 0.453965 3_2:-59316116 None:intergenic
AACACAAGACTCTTTCCATC+GGG 0.458022 3_2:-59316517 None:intergenic
ATATATTTGGGTGACCAAGC+AGG 0.462087 3_2:-59316679 None:intergenic
TAATGATGCGGGTTAGAGAT+AGG 0.463618 3_2:-59316571 None:intergenic
ACGGTGAATATTTCACCATA+AGG 0.464654 3_2:-59316598 None:intergenic
CATAGTGTGCTGGATGTGTT+AGG 0.466258 3_2:+59314883 Msa0414250:CDS
GGGTGTTACCAGATTTGAGA+TGG 0.466658 3_2:-59315301 None:intergenic
TGGACCTTATTGTTCATTTG+AGG 0.466996 3_2:+59316732 Msa0414250:CDS
CCTGGTTAACAATGAATTCA+AGG 0.470606 3_2:+59315745 Msa0414250:CDS
GTTCATAAATGAACAATCAA+TGG 0.479217 3_2:+59313558 Msa0414250:CDS
CCGGTGTACCACGTGTCAGC+TGG 0.479911 3_2:+59315261 Msa0414250:CDS
TTTCTCAACCGCATGGGACC+GGG 0.485033 3_2:+59316057 Msa0414250:CDS
CCTTGAATTCATTGTTAACC+AGG 0.485527 3_2:-59315745 None:intergenic
ATCGGAGAGGGAACGACGGT+TGG 0.487237 3_2:+59315546 Msa0414250:CDS
ATCGCACTATTTACTTTCAT+CGG 0.488789 3_2:+59315528 Msa0414250:CDS
GTTTGAGATGTAAGAGGCAA+AGG 0.488923 3_2:-59314527 None:intergenic
AAGTAGTTGAGCACGGTGGC+TGG 0.490938 3_2:+59315489 Msa0414250:CDS
CCAAACTACATTGTTAGTAC+AGG 0.491243 3_2:+59316778 Msa0414250:CDS
GGGTTGAGATGGTGAGCATT+GGG 0.494084 3_2:-59315788 None:intergenic
CGCCTGATTCAACGCGGGTC+GGG 0.494143 3_2:+59315819 Msa0414250:CDS
ATGGAGAAAATACCTTCAAA+AGG 0.494795 3_2:-59314560 None:intergenic
GGTCGGATACAATGTGCAAC+TGG 0.496813 3_2:+59316341 Msa0414250:CDS
CGGGTTGAGATGGTGAGCAT+TGG 0.503026 3_2:-59315789 None:intergenic
GTGCGATCGTCCACCCAACT+TGG 0.507384 3_2:-59315512 None:intergenic
AGAATTGGTCAGTTCGTCAC+AGG 0.513528 3_2:+59314064 Msa0414250:CDS
GTCGCTTCCTATGGTGGTAA+AGG 0.515139 3_2:+59315392 Msa0414250:CDS
GTGTTTACGTCAGATTATGC+AGG 0.515147 3_2:+59316535 Msa0414250:CDS
GTTCATTTGAGGATTGCCAT+TGG 0.516301 3_2:+59316743 Msa0414250:CDS
GAACAATAAGGTCCACCCTT+AGG 0.516534 3_2:-59316724 None:intergenic
CTAACTCTCTTCCGGTTCAC+CGG 0.516799 3_2:+59315920 Msa0414250:CDS
GATTAAATACTCACCGTCGC+TGG 0.518095 3_2:-59315216 None:intergenic
ATCGCTTACGTTGAAATGCC+TGG 0.518227 3_2:+59315977 Msa0414250:CDS
ATTTCCATGGGAAGGTTTGA+CGG 0.519457 3_2:-59313632 None:intergenic
TATGGTGGTAAAGGTTGGTC+CGG 0.521256 3_2:+59315401 Msa0414250:CDS
TCACCGTCGCTGGTGACACT+TGG 0.521972 3_2:-59315206 None:intergenic
GTGCCCCTTAAAAGAACTGA+TGG 0.523442 3_2:-59314790 None:intergenic
GCCGTAGGGAGTGAGTCTTC+GGG 0.524195 3_2:-59315321 None:intergenic
TTTCCGTCTGGCGATGGTGA+CGG 0.524911 3_2:-59316214 None:intergenic
AGCCCCGCTGTATCTCCTAC+CGG 0.526308 3_2:+59315356 Msa0414250:CDS
ACTGGTCCCCTGATGGAGAA+TGG 0.526653 3_2:+59316359 Msa0414250:CDS
GCTAAAATCAGCGACGCCGT+AGG 0.527113 3_2:-59315336 None:intergenic
CCAGCACACTATGGGTTTGG+AGG 0.527330 3_2:-59314873 None:intergenic
CTAAAATCAGCGACGCCGTA+GGG 0.528018 3_2:-59315335 None:intergenic
TGGACAGCAGTCGCTTCCTA+TGG 0.528066 3_2:+59315383 Msa0414250:CDS
TTAGGCCTCTCAACATTCAC+AGG 0.529361 3_2:-59316706 None:intergenic
TGCATCCGATCGTCATGACC+CGG 0.530784 3_2:+59316390 Msa0414250:CDS
GGAAGTGTTTGAGATGTAAG+AGG 0.531897 3_2:-59314533 None:intergenic
CTATTTACTTTCATCGGAGA+GGG 0.531904 3_2:+59315534 Msa0414250:CDS
GCGAGATAAACGGGTTGAGA+TGG 0.532136 3_2:-59315799 None:intergenic
TGAAATATTCACCGTGAGGT+TGG 0.532205 3_2:+59316606 Msa0414250:CDS
ATTACATTATTTCCATGGGA+AGG 0.532230 3_2:-59313640 None:intergenic
ATATTCACCGTGAGGTTGGA+TGG 0.533164 3_2:+59316610 Msa0414250:CDS
CTCTAGCATAAACTGAGGAC+TGG 0.534919 3_2:-59315873 None:intergenic
TGTATGCAGCGAGTTGAAGA+AGG 0.535311 3_2:+59313996 Msa0414250:intron
CTTCCTATGGTGGTAAAGGT+TGG 0.535859 3_2:+59315396 Msa0414250:CDS
GAAATTTCGGTCAACATGGA+TGG 0.535963 3_2:-59316152 None:intergenic
TATATTTGGGTGACCAAGCA+GGG 0.537415 3_2:-59316678 None:intergenic
CATAAGGTTGATAATGATGC+GGG 0.540407 3_2:-59316582 None:intergenic
ATGAAGATGGAACCCCTGCT+TGG 0.542282 3_2:+59316665 Msa0414250:CDS
TCGCCTGATTCAACGCGGGT+CGG 0.542292 3_2:+59315818 Msa0414250:CDS
AGCTCCCATATGACAATACA+AGG 0.542449 3_2:-59314694 None:intergenic
TTTCCGGTAGGAGATACAGC+GGG 0.542707 3_2:-59315359 None:intergenic
GTGTCAACGCACGAGGATCC+CGG 0.545801 3_2:+59315242 Msa0414250:CDS
AACAGAGTTAGAAGAATCAG+TGG 0.546517 3_2:-59315594 None:intergenic
TCTCAAAGCTTCCACTTCCC+GGG 0.551863 3_2:-59316408 None:intergenic
AGTGTAATCTACACAGCAGT+TGG 0.553324 3_2:+59316088 Msa0414250:CDS
CTATATTACATTATTTCCAT+GGG 0.553660 3_2:-59313644 None:intergenic
CAGCTGACACGTGGTACACC+GGG 0.557055 3_2:-59315260 None:intergenic
CGGTGGCTGGCCAAGTTGGG+TGG 0.557379 3_2:+59315502 Msa0414250:CDS
AAAATACCTTCAAAAGGCAT+AGG 0.557495 3_2:-59314554 None:intergenic
GTCAAGAAGTTGACCCTAGA+CGG 0.557743 3_2:+59316175 Msa0414250:CDS
GCATCCGATCGTCATGACCC+GGG 0.558569 3_2:+59316391 Msa0414250:CDS
GGTGAGTTCCGTCGTGAGTG+AGG 0.560303 3_2:-59315460 None:intergenic
GGTGTAAACGCGCGTAAACC+CGG 0.562278 3_2:-59315638 None:intergenic
GGTGTTACCAGATTTGAGAT+GGG 0.562870 3_2:-59315300 None:intergenic
CCTCCAAACCCATAGTGTGC+TGG 0.563175 3_2:+59314873 Msa0414250:CDS
TAACACATCCAGCACACTAT+GGG 0.563262 3_2:-59314881 None:intergenic
AGAGATTAACAGAGGGTCCA+TGG 0.564220 3_2:+59316320 Msa0414250:CDS
GAAGAGAGTTAGATTTGGAA+CGG 0.564617 3_2:-59315909 None:intergenic
TGATCCACCCGAATGGAACC+GGG 0.566439 3_2:+59316437 Msa0414250:CDS
TGACGAAATTTCGGTCAACA+TGG 0.566872 3_2:-59316156 None:intergenic
TTGAATCAGTTTCCTTAACC+CGG 0.567806 3_2:-59316455 None:intergenic
TTTGAGATGGGTGGAATAGA+CGG 0.568439 3_2:-59315288 None:intergenic
CCAGCTGACACGTGGTACAC+CGG 0.569828 3_2:-59315261 None:intergenic
CTGATTCAAAGTGGTTCAGG+TGG 0.570012 3_2:+59316469 Msa0414250:CDS
ATGTGACGGAAGCTAGAGCC+AGG 0.571700 3_2:-59315710 None:intergenic
CAATGGCTTTCTCAACCGCA+TGG 0.572392 3_2:+59316050 Msa0414250:CDS
GGGTTTACGCGCGTTTACAC+CGG 0.572546 3_2:+59315640 Msa0414250:CDS
GTCTGGCGATGGTGACGGAA+AGG 0.573602 3_2:-59316209 None:intergenic
GGAATGCTGTTGGTAGGAGA+TGG 0.576345 3_2:-59314506 None:intergenic
TTGGCATGTTGTTGAGCCAA+TGG 0.579141 3_2:-59316759 None:intergenic
CGTGCTCAACTACTTTGTAC+CGG 0.581884 3_2:-59315481 None:intergenic
ATTTATTTGATCCACCCGAA+TGG 0.583814 3_2:+59316430 Msa0414250:CDS
GCCGTCGCAACGAGAAGACC+TGG 0.584072 3_2:+59315692 Msa0414250:CDS
CGGTTCACTACGTAAACACC+AGG 0.585044 3_2:-59315995 None:intergenic
AGTGAGCTCCTCTACTTCGC+CGG 0.586849 3_2:-59315420 None:intergenic
TTAACCCGGTTCCATTCGGG+TGG 0.587009 3_2:-59316441 None:intergenic
ATGTGCAACTGGTCCCCTGA+TGG 0.587384 3_2:+59316352 Msa0414250:CDS
AGGTTGGTCCGGCGAAGTAG+AGG 0.590368 3_2:+59315412 Msa0414250:CDS
GTTACCAGATTTGAGATGGG+TGG 0.590420 3_2:-59315297 None:intergenic
TATTGAGGAACAATTTCTTG+AGG 0.592474 3_2:+59314454 Msa0414250:CDS
AAACACAAGACTCTTTCCAT+CGG 0.593429 3_2:-59316518 None:intergenic
TTCAAAGTGGTTCAGGTGGG+AGG 0.597857 3_2:+59316473 Msa0414250:CDS
ACCAATTCTAAATGAGCTGA+GGG 0.598408 3_2:-59314050 None:intergenic
GACCAATTCTAAATGAGCTG+AGG 0.600904 3_2:-59314051 None:intergenic
CCATAAGGTTGATAATGATG+CGG 0.601624 3_2:-59316583 None:intergenic
TGGTGTTTACGTAGTGAACC+GGG 0.601869 3_2:+59315997 Msa0414250:CDS
GCCAGGTCTTCTCGTTGCGA+CGG 0.603694 3_2:-59315693 None:intergenic
CAAACTACATTGTTAGTACA+GGG 0.603915 3_2:+59316779 Msa0414250:CDS
GGACCAACCTTTACCACCAT+AGG 0.605783 3_2:-59315399 None:intergenic
AAGCGATCCATTCTCCATCA+GGG 0.608545 3_2:-59316366 None:intergenic
TGTGAATGTTGAGAGGCCTA+AGG 0.611126 3_2:+59316708 Msa0414250:CDS
ACTTCCCGGGTCATGACGAT+CGG 0.612635 3_2:-59316395 None:intergenic
TCACATCGAATTGTTCGACC+TGG 0.614521 3_2:+59315727 Msa0414250:CDS
ATCGAACGAGTTACACCACC+GGG 0.614798 3_2:+59315620 Msa0414250:CDS
ACAGCAGTCGCTTCCTATGG+TGG 0.615062 3_2:+59315386 Msa0414250:CDS
ACCTGAACCATCCAACCTCA+CGG 0.618847 3_2:-59316617 None:intergenic
TCACTCACGACGGAACTCAC+CGG 0.621307 3_2:+59315462 Msa0414250:CDS
AATCTACGTGTCAACGCACG+AGG 0.623806 3_2:+59315235 Msa0414250:CDS
AGATTACACTAGGTCGAACC+CGG 0.625928 3_2:-59316075 None:intergenic
GGCAAAGGAATGCTGTTGGT+AGG 0.627841 3_2:-59314512 None:intergenic
AATGGCTTTCTCAACCGCAT+GGG 0.628096 3_2:+59316051 Msa0414250:CDS
ACTATTTACTTTCATCGGAG+AGG 0.631119 3_2:+59315533 Msa0414250:CDS
ACCCGAAGACTCACTCCCTA+CGG 0.633343 3_2:+59315320 Msa0414250:CDS
GTGAATGTTGAGAGGCCTAA+GGG 0.633649 3_2:+59316709 Msa0414250:CDS
GGTCGAACAATTCGATGTGA+CGG 0.634875 3_2:-59315724 None:intergenic
ATTAACAGAGGGTCCATGGT+CGG 0.636917 3_2:+59316324 Msa0414250:CDS
AAGAGGCAAAGGAATGCTGT+TGG 0.643088 3_2:-59314516 None:intergenic
GTCGGGTATCACAAGTGCAG+AGG 0.643154 3_2:+59315836 Msa0414250:CDS
TTTCCGTCACCATCGCCAGA+CGG 0.643830 3_2:+59316211 Msa0414250:CDS
TATCTCGCCTGATTCAACGC+GGG 0.645965 3_2:+59315814 Msa0414250:CDS
CATCGAACGAGTTACACCAC+CGG 0.646119 3_2:+59315619 Msa0414250:CDS
TTAAGGAAACTGATTCAAAG+TGG 0.647288 3_2:+59316460 Msa0414250:CDS
AGCGATCCATTCTCCATCAG+GGG 0.647416 3_2:-59316365 None:intergenic
AAACCAAGTGTCACCAGCGA+CGG 0.647626 3_2:+59315203 Msa0414250:CDS
ATATTTGGGTGACCAAGCAG+GGG 0.655465 3_2:-59316677 None:intergenic
TTGCACATTGTATCCGACCA+TGG 0.655497 3_2:-59316337 None:intergenic
ACTGCTGTGTAGATTACACT+AGG 0.657407 3_2:-59316085 None:intergenic
TGATTCAAAGTGGTTCAGGT+GGG 0.658589 3_2:+59316470 Msa0414250:CDS
TCAAGCCTGTGAATGTTGAG+AGG 0.661021 3_2:+59316701 Msa0414250:CDS
ACTACCGTCAAACCTTCCCA+TGG 0.662089 3_2:+59313628 Msa0414250:CDS
GAACAAGATGTTGATGTCAG+AGG 0.666305 3_2:+59314268 Msa0414250:CDS
TAAGCGATTCTGTCTCCAGA+GGG 0.666512 3_2:-59315962 None:intergenic
CGGTACAAAGTAGTTGAGCA+CGG 0.671883 3_2:+59315482 Msa0414250:CDS
AGGTCGAACCCGGTCCCATG+CGG 0.680107 3_2:-59316065 None:intergenic
TATGACCTGATCGACCAGAA+CGG 0.684219 3_2:-59316249 None:intergenic
AGAGAGTTAGATTTGGAACG+GGG 0.688808 3_2:-59315907 None:intergenic
GTAAGCGATTCTGTCTCCAG+AGG 0.689173 3_2:-59315963 None:intergenic
GTTTACGTAGTGAACCGGGA+CGG 0.690796 3_2:+59316001 Msa0414250:CDS
ATGGTGAAATATTCACCGTG+AGG 0.692339 3_2:+59316602 Msa0414250:CDS
ACGGCAGTCCAGCTGACACG+TGG 0.692685 3_2:-59315269 None:intergenic
CGGGCTTAAGAGATTAACAG+AGG 0.696246 3_2:+59316312 Msa0414250:CDS
CTTTGCTTCAGAAAGCACTG+AGG 0.697797 3_2:+59316117 Msa0414250:CDS
TTCCGGTAGGAGATACAGCG+GGG 0.706099 3_2:-59315358 None:intergenic
GGGCTTAAGAGATTAACAGA+GGG 0.706815 3_2:+59316313 Msa0414250:CDS
AATGTTGAGAGGCCTAAGGG+TGG 0.712549 3_2:+59316712 Msa0414250:CDS
TTTCATCGGAGAGGGAACGA+CGG 0.712613 3_2:+59315542 Msa0414250:CDS
TTATCTCGCCTGATTCAACG+CGG 0.721298 3_2:+59315813 Msa0414250:CDS
ACAGGGCTACATGCACAATG+TGG 0.743092 3_2:+59316796 Msa0414250:CDS
GTAAACGCGCGTAAACCCGG+TGG 0.749810 3_2:-59315635 None:intergenic
ACATTCTCTAGCATAAACTG+AGG 0.757075 3_2:-59315878 None:intergenic
ACACAAGACTCTTTCCATCG+GGG 0.760389 3_2:-59316516 None:intergenic
TACAAAGTAGTTGAGCACGG+TGG 0.792659 3_2:+59315485 Msa0414250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGATATAAGAAAAATA+GGG - chr3_2:59313921-59313940 None:intergenic 10.0%
!! TAAAAAGATATAAGAAAAAT+AGG - chr3_2:59313922-59313941 None:intergenic 10.0%
!! AATTAAAAGTAAGTTTAATT+TGG - chr3_2:59313977-59313996 None:intergenic 10.0%
!! ATTGAAAATATATATAAGTT+TGG - chr3_2:59314197-59314216 None:intergenic 10.0%
!!! TTAATTTAATGATTTTGTTT+TGG + chr3_2:59314587-59314606 Msa0414250:intron 10.0%
!! AGATTAAAAATAGAAAACAA+AGG - chr3_2:59314150-59314169 None:intergenic 15.0%
!! CTATATTACATTATTTCCAT+GGG - chr3_2:59313647-59313666 None:intergenic 20.0%
!! ACTATATTACATTATTTCCA+TGG - chr3_2:59313648-59313667 None:intergenic 20.0%
!!! TTTAGCTTTAGATGAAAATA+AGG - chr3_2:59313675-59313694 None:intergenic 20.0%
!!! TTGTTTATTTCTATATGTGT+AGG + chr3_2:59314246-59314265 Msa0414250:intron 20.0%
!! TCATAAGAATCTTTACTTAA+TGG + chr3_2:59316267-59316286 Msa0414250:CDS 20.0%
! TCATATTTCTTTCTTGTTAG+AGG + chr3_2:59313534-59313553 Msa0414250:CDS 25.0%
! GTTCATAAATGAACAATCAA+TGG + chr3_2:59313558-59313577 Msa0414250:CDS 25.0%
! TATATAAGTTTGGAAAAGCT+CGG - chr3_2:59314187-59314206 None:intergenic 25.0%
!! ACAAATGAATATAGTTCTAC+TGG - chr3_2:59314230-59314249 None:intergenic 25.0%
! GTTTAACTCCAAAATCAAAA+GGG - chr3_2:59314370-59314389 None:intergenic 25.0%
! AGTTTAACTCCAAAATCAAA+AGG - chr3_2:59314371-59314390 None:intergenic 25.0%
! ATTTAGAGAAACTGAAATAG+TGG - chr3_2:59314405-59314424 None:intergenic 25.0%
! TCAATTAAGCTCAATTATTG+AGG + chr3_2:59314439-59314458 Msa0414250:CDS 25.0%
!! AATTTTCCACTATTTCAGAA+GGG - chr3_2:59314666-59314685 None:intergenic 25.0%
! TTGAATATTGTCTCAAAAAG+AGG - chr3_2:59315172-59315191 None:intergenic 25.0%
!!! TCTTTTTGAGACAATATTCA+AGG + chr3_2:59315171-59315190 Msa0414250:intron 25.0%
! ACTCATAATTCTTATGAAGA+TGG + chr3_2:59316652-59316671 Msa0414250:CDS 25.0%
ATTACATTATTTCCATGGGA+AGG - chr3_2:59313643-59313662 None:intergenic 30.0%
!! AGCTTTAGATGAAAATAAGG+CGG - chr3_2:59313672-59313691 None:intergenic 30.0%
! TTGCACAGTATTGAATACTT+TGG - chr3_2:59313803-59313822 None:intergenic 30.0%
!! ACAACTTTTTCCACCATTTT+AGG + chr3_2:59313843-59313862 Msa0414250:intron 30.0%
!! CAACTTTTTCCACCATTTTA+GGG + chr3_2:59313844-59313863 Msa0414250:intron 30.0%
TTAGGAGCAACTAATTGTAT+AGG - chr3_2:59313885-59313904 None:intergenic 30.0%
AAAAGCTCGGATTCATTTAT+AGG - chr3_2:59314174-59314193 None:intergenic 30.0%
! ACATTGGTCATTATTAGTCT+TGG - chr3_2:59314308-59314327 None:intergenic 30.0%
TATTGAGGAACAATTTCTTG+AGG + chr3_2:59314454-59314473 Msa0414250:CDS 30.0%
! AAAATACCTTCAAAAGGCAT+AGG - chr3_2:59314557-59314576 None:intergenic 30.0%
ATGGAGAAAATACCTTCAAA+AGG - chr3_2:59314563-59314582 None:intergenic 30.0%
! TCAACTATGCTTTTATTCAC+AGG + chr3_2:59314622-59314641 Msa0414250:intron 30.0%
!! ATTAAACCCTTCTGAAATAG+TGG + chr3_2:59314657-59314676 Msa0414250:CDS 30.0%
! GAATTTTCCACTATTTCAGA+AGG - chr3_2:59314667-59314686 None:intergenic 30.0%
TTCTCCTTGTATTGTCATAT+GGG + chr3_2:59314690-59314709 Msa0414250:CDS 30.0%
CTCGTAATCCAAATAATTCT+CGG - chr3_2:59314734-59314753 None:intergenic 30.0%
! AGTATCCATCAGTTCTTTTA+AGG + chr3_2:59314785-59314804 Msa0414250:CDS 30.0%
! GTATCCATCAGTTCTTTTAA+GGG + chr3_2:59314786-59314805 Msa0414250:CDS 30.0%
! TATCCATCAGTTCTTTTAAG+GGG + chr3_2:59314787-59314806 Msa0414250:CDS 30.0%
!! AGACATTTTAAGTCAACATG+TGG + chr3_2:59315095-59315114 Msa0414250:intron 30.0%
ATCGCACTATTTACTTTCAT+CGG + chr3_2:59315528-59315547 Msa0414250:CDS 30.0%
TGAAAAAAAAGGAAACGAAC+CGG - chr3_2:59315942-59315961 None:intergenic 30.0%
!!! TTGCATCAGAAACCTTTTTT+CGG - chr3_2:59316033-59316052 None:intergenic 30.0%
AAAAAAGGTTTCTGATGCAA+TGG + chr3_2:59316033-59316052 Msa0414250:CDS 30.0%
TCAACTTCTTGACGAAATTT+CGG - chr3_2:59316168-59316187 None:intergenic 30.0%
! TTAAGGAAACTGATTCAAAG+TGG + chr3_2:59316460-59316479 Msa0414250:CDS 30.0%
TTCACAGGCTTGATATATTT+GGG - chr3_2:59316694-59316713 None:intergenic 30.0%
ATTCACAGGCTTGATATATT+TGG - chr3_2:59316695-59316714 None:intergenic 30.0%
CAATCCTCAAATGAACAATA+AGG - chr3_2:59316739-59316758 None:intergenic 30.0%
CAAACTACATTGTTAGTACA+GGG + chr3_2:59316779-59316798 Msa0414250:CDS 30.0%
ATTAGTTGCTCCTAAACCAA+AGG + chr3_2:59313890-59313909 Msa0414250:intron 35.0%
GAAAAATAGGGAAATGCCTT+TGG - chr3_2:59313909-59313928 None:intergenic 35.0%
ACCAATTCTAAATGAGCTGA+GGG - chr3_2:59314053-59314072 None:intergenic 35.0%
TGACCAATGTAAAGACTCTT+AGG + chr3_2:59314318-59314337 Msa0414250:intron 35.0%
CATCCTAAGAGTCTTTACAT+TGG - chr3_2:59314324-59314343 None:intergenic 35.0%
CCAAAATCAAAAGGGTTATG+AGG - chr3_2:59314362-59314381 None:intergenic 35.0%
!!! CCTCATAACCCTTTTGATTT+TGG + chr3_2:59314359-59314378 Msa0414250:intron 35.0%
!!! ATTTTGTTTTGGTTCTCGAG+AGG + chr3_2:59314598-59314617 Msa0414250:intron 35.0%
! TATGCTTTTATTCACAGGTG+AGG + chr3_2:59314627-59314646 Msa0414250:intron 35.0%
GTTCTCCTTGTATTGTCATA+TGG + chr3_2:59314689-59314708 Msa0414250:CDS 35.0%
!!! GCTTTGGTTTTGATAAGGAT+TGG + chr3_2:59314950-59314969 Msa0414250:CDS 35.0%
!!! TGGTTTTGATAAGGATTGGT+TGG + chr3_2:59314954-59314973 Msa0414250:CDS 35.0%
AGATGCATTGCAAAAACTGT+TGG + chr3_2:59315023-59315042 Msa0414250:CDS 35.0%
!!! AGTTTTAGAAACAGTCTCAG+AGG - chr3_2:59315145-59315164 None:intergenic 35.0%
ACTATTTACTTTCATCGGAG+AGG + chr3_2:59315533-59315552 Msa0414250:CDS 35.0%
CTATTTACTTTCATCGGAGA+GGG + chr3_2:59315534-59315553 Msa0414250:CDS 35.0%
AACAGAGTTAGAAGAATCAG+TGG - chr3_2:59315597-59315616 None:intergenic 35.0%
CCTTGAATTCATTGTTAACC+AGG - chr3_2:59315748-59315767 None:intergenic 35.0%
CCTGGTTAACAATGAATTCA+AGG + chr3_2:59315745-59315764 Msa0414250:CDS 35.0%
ACATTCTCTAGCATAAACTG+AGG - chr3_2:59315881-59315900 None:intergenic 35.0%
! AAGAGAGTTAGATTTGGAAC+GGG - chr3_2:59315911-59315930 None:intergenic 35.0%
GAAGAGAGTTAGATTTGGAA+CGG - chr3_2:59315912-59315931 None:intergenic 35.0%
TTCCAAATCTAACTCTCTTC+CGG + chr3_2:59315912-59315931 Msa0414250:CDS 35.0%
!!! TTTCCTTTTTTTTCACCCTC+TGG + chr3_2:59315947-59315966 Msa0414250:CDS 35.0%
! AAAACAATCCATTTTCCGTC+TGG - chr3_2:59316229-59316248 None:intergenic 35.0%
!!! AAATGGATTGTTTTCCGTTC+TGG + chr3_2:59316235-59316254 Msa0414250:CDS 35.0%
ATTTATTTGATCCACCCGAA+TGG + chr3_2:59316430-59316449 Msa0414250:CDS 35.0%
TTGAATCAGTTTCCTTAACC+CGG - chr3_2:59316458-59316477 None:intergenic 35.0%
! AAACTGATTCAAAGTGGTTC+AGG + chr3_2:59316466-59316485 Msa0414250:CDS 35.0%
AAACACAAGACTCTTTCCAT+CGG - chr3_2:59316521-59316540 None:intergenic 35.0%
!! CATAAGGTTGATAATGATGC+GGG - chr3_2:59316585-59316604 None:intergenic 35.0%
!! CCATAAGGTTGATAATGATG+CGG - chr3_2:59316586-59316605 None:intergenic 35.0%
ACGGTGAATATTTCACCATA+AGG - chr3_2:59316601-59316620 None:intergenic 35.0%
TGGACCTTATTGTTCATTTG+AGG + chr3_2:59316732-59316751 Msa0414250:CDS 35.0%
CCTGTACTAACAATGTAGTT+TGG - chr3_2:59316781-59316800 None:intergenic 35.0%
CCAAACTACATTGTTAGTAC+AGG + chr3_2:59316778-59316797 Msa0414250:CDS 35.0%
ATTTCCATGGGAAGGTTTGA+CGG - chr3_2:59313635-59313654 None:intergenic 40.0%
CTTTCACAAACTGTGTCACA+TGG - chr3_2:59313770-59313789 None:intergenic 40.0%
!! TTTCCACCATTTTAGGGACA+AGG + chr3_2:59313850-59313869 Msa0414250:intron 40.0%
ATTCCTTGTCCCTAAAATGG+TGG - chr3_2:59313856-59313875 None:intergenic 40.0%
CTCATTCCTTGTCCCTAAAA+TGG - chr3_2:59313859-59313878 None:intergenic 40.0%
GGAGCAACTAATTGTATAGG+TGG - chr3_2:59313882-59313901 None:intergenic 40.0%
! TAGGGAAATGCCTTTGGTTT+AGG - chr3_2:59313903-59313922 None:intergenic 40.0%
! ACCCTCAGCTCATTTAGAAT+TGG + chr3_2:59314049-59314068 Msa0414250:CDS 40.0%
GACCAATTCTAAATGAGCTG+AGG - chr3_2:59314054-59314073 None:intergenic 40.0%
GAACAAGATGTTGATGTCAG+AGG + chr3_2:59314268-59314287 Msa0414250:CDS 40.0%
! GTTTGAGATGTAAGAGGCAA+AGG - chr3_2:59314530-59314549 None:intergenic 40.0%
!! GGAAGTGTTTGAGATGTAAG+AGG - chr3_2:59314536-59314555 None:intergenic 40.0%
! ACACTTCCTATGCCTTTTGA+AGG + chr3_2:59314548-59314567 Msa0414250:CDS 40.0%
AGCTCCCATATGACAATACA+AGG - chr3_2:59314697-59314716 None:intergenic 40.0%
! ACTGGTCTCCGAGAATTATT+TGG + chr3_2:59314723-59314742 Msa0414250:CDS 40.0%
TAACACATCCAGCACACTAT+GGG - chr3_2:59314884-59314903 None:intergenic 40.0%
! TGAAAGACGTGCTCTGTTTT+CGG + chr3_2:59314984-59315003 Msa0414250:CDS 40.0%
ACGCAACACTTCAACTTCTT+TGG - chr3_2:59315067-59315086 None:intergenic 40.0%
! TTTGAGATGGGTGGAATAGA+CGG - chr3_2:59315291-59315310 None:intergenic 40.0%
TATTCCACCCATCTCAAATC+TGG + chr3_2:59315293-59315312 Msa0414250:CDS 40.0%
! GGTGTTACCAGATTTGAGAT+GGG - chr3_2:59315303-59315322 None:intergenic 40.0%
!! GCGATGAATTTGTTGTTTCC+AGG - chr3_2:59315674-59315693 None:intergenic 40.0%
TTGAATCAGGCGAGATAAAC+GGG - chr3_2:59315811-59315830 None:intergenic 40.0%
GTTGAATCAGGCGAGATAAA+CGG - chr3_2:59315812-59315831 None:intergenic 40.0%
! AGAGAGTTAGATTTGGAACG+GGG - chr3_2:59315910-59315929 None:intergenic 40.0%
AACCGGAAGAGAGTTAGATT+TGG - chr3_2:59315917-59315936 None:intergenic 40.0%
TCTCCAGAGGGTGAAAAAAA+AGG - chr3_2:59315953-59315972 None:intergenic 40.0%
ACTGCTGTGTAGATTACACT+AGG - chr3_2:59316088-59316107 None:intergenic 40.0%
AGTGTAATCTACACAGCAGT+TGG + chr3_2:59316088-59316107 Msa0414250:CDS 40.0%
GAAATTTCGGTCAACATGGA+TGG - chr3_2:59316155-59316174 None:intergenic 40.0%
TGACGAAATTTCGGTCAACA+TGG - chr3_2:59316159-59316178 None:intergenic 40.0%
! TACTTAATGGATGCCGTTCA+CGG + chr3_2:59316280-59316299 Msa0414250:CDS 40.0%
GGGCTTAAGAGATTAACAGA+GGG + chr3_2:59316313-59316332 Msa0414250:CDS 40.0%
TGATTCAAAGTGGTTCAGGT+GGG + chr3_2:59316470-59316489 Msa0414250:CDS 40.0%
TTTCCATCGGGGCTAAAATA+TGG - chr3_2:59316508-59316527 None:intergenic 40.0%
AACACAAGACTCTTTCCATC+GGG - chr3_2:59316520-59316539 None:intergenic 40.0%
GTGTTTACGTCAGATTATGC+AGG + chr3_2:59316535-59316554 Msa0414250:CDS 40.0%
!! TAATGATGCGGGTTAGAGAT+AGG - chr3_2:59316574-59316593 None:intergenic 40.0%
CCGCATCATTATCAACCTTA+TGG + chr3_2:59316583-59316602 Msa0414250:CDS 40.0%
ATGGTGAAATATTCACCGTG+AGG + chr3_2:59316602-59316621 Msa0414250:CDS 40.0%
TGAAATATTCACCGTGAGGT+TGG + chr3_2:59316606-59316625 Msa0414250:CDS 40.0%
TATATTTGGGTGACCAAGCA+GGG - chr3_2:59316681-59316700 None:intergenic 40.0%
ATATATTTGGGTGACCAAGC+AGG - chr3_2:59316682-59316701 None:intergenic 40.0%
GTTCATTTGAGGATTGCCAT+TGG + chr3_2:59316743-59316762 Msa0414250:CDS 40.0%
! TGTATGCAGCGAGTTGAAGA+AGG + chr3_2:59313996-59314015 Msa0414250:intron 45.0%
AGAATTGGTCAGTTCGTCAC+AGG + chr3_2:59314064-59314083 Msa0414250:CDS 45.0%
AAGAGGCAAAGGAATGCTGT+TGG - chr3_2:59314519-59314538 None:intergenic 45.0%
GTGCCCCTTAAAAGAACTGA+TGG - chr3_2:59314793-59314812 None:intergenic 45.0%
CTAACACATCCAGCACACTA+TGG - chr3_2:59314885-59314904 None:intergenic 45.0%
CATAGTGTGCTGGATGTGTT+AGG + chr3_2:59314883-59314902 Msa0414250:CDS 45.0%
CTACACAATTTGCGCAGCTT+TGG + chr3_2:59314934-59314953 Msa0414250:CDS 45.0%
!!! GCGCAGCTTTGGTTTTGATA+AGG + chr3_2:59314945-59314964 Msa0414250:CDS 45.0%
GATTAAATACTCACCGTCGC+TGG - chr3_2:59315219-59315238 None:intergenic 45.0%
! GTTACCAGATTTGAGATGGG+TGG - chr3_2:59315300-59315319 None:intergenic 45.0%
! GGGTGTTACCAGATTTGAGA+TGG - chr3_2:59315304-59315323 None:intergenic 45.0%
! TTTTCCGGTAGGAGATACAG+CGG - chr3_2:59315363-59315382 None:intergenic 45.0%
CTGTATCTCCTACCGGAAAA+TGG + chr3_2:59315363-59315382 Msa0414250:CDS 45.0%
CTTCCTATGGTGGTAAAGGT+TGG + chr3_2:59315396-59315415 Msa0414250:CDS 45.0%
! TATGGTGGTAAAGGTTGGTC+CGG + chr3_2:59315401-59315420 Msa0414250:CDS 45.0%
! TACGTTTTCCTCACTCACGA+CGG + chr3_2:59315452-59315471 Msa0414250:CDS 45.0%
CGTGCTCAACTACTTTGTAC+CGG - chr3_2:59315484-59315503 None:intergenic 45.0%
CGGTACAAAGTAGTTGAGCA+CGG + chr3_2:59315482-59315501 Msa0414250:CDS 45.0%
TACAAAGTAGTTGAGCACGG+TGG + chr3_2:59315485-59315504 Msa0414250:CDS 45.0%
TTGTTTCCAGGTGAAGTTGC+CGG - chr3_2:59315662-59315681 None:intergenic 45.0%
GGTCGAACAATTCGATGTGA+CGG - chr3_2:59315727-59315746 None:intergenic 45.0%
TCACATCGAATTGTTCGACC+TGG + chr3_2:59315727-59315746 Msa0414250:CDS 45.0%
TTATCTCGCCTGATTCAACG+CGG + chr3_2:59315813-59315832 Msa0414250:CDS 45.0%
CTCTAGCATAAACTGAGGAC+TGG - chr3_2:59315876-59315895 None:intergenic 45.0%
TAAGCGATTCTGTCTCCAGA+GGG - chr3_2:59315965-59315984 None:intergenic 45.0%
ATCGCTTACGTTGAAATGCC+TGG + chr3_2:59315977-59315996 Msa0414250:CDS 45.0%
CTGGTGTTTACGTAGTGAAC+CGG + chr3_2:59315996-59316015 Msa0414250:CDS 45.0%
TGGTGTTTACGTAGTGAACC+GGG + chr3_2:59315997-59316016 Msa0414250:CDS 45.0%
AATGGCTTTCTCAACCGCAT+GGG + chr3_2:59316051-59316070 Msa0414250:CDS 45.0%
AGATTACACTAGGTCGAACC+CGG - chr3_2:59316078-59316097 None:intergenic 45.0%
!! CTCAGTGCTTTCTGAAGCAA+AGG - chr3_2:59316119-59316138 None:intergenic 45.0%
!! CTTTGCTTCAGAAAGCACTG+AGG + chr3_2:59316117-59316136 Msa0414250:CDS 45.0%
GTCAAGAAGTTGACCCTAGA+CGG + chr3_2:59316175-59316194 Msa0414250:CDS 45.0%
GGCATTGTTCTTTCCGTCTA+GGG - chr3_2:59316191-59316210 None:intergenic 45.0%
! AGGCATTGTTCTTTCCGTCT+AGG - chr3_2:59316192-59316211 None:intergenic 45.0%
TATGACCTGATCGACCAGAA+CGG - chr3_2:59316252-59316271 None:intergenic 45.0%
CGGGCTTAAGAGATTAACAG+AGG + chr3_2:59316312-59316331 Msa0414250:CDS 45.0%
AGAGATTAACAGAGGGTCCA+TGG + chr3_2:59316320-59316339 Msa0414250:CDS 45.0%
ATTAACAGAGGGTCCATGGT+CGG + chr3_2:59316324-59316343 Msa0414250:CDS 45.0%
TTGCACATTGTATCCGACCA+TGG - chr3_2:59316340-59316359 None:intergenic 45.0%
AAGCGATCCATTCTCCATCA+GGG - chr3_2:59316369-59316388 None:intergenic 45.0%
AAAGCGATCCATTCTCCATC+AGG - chr3_2:59316370-59316389 None:intergenic 45.0%
ATCTCAAAGCTTCCACTTCC+CGG - chr3_2:59316412-59316431 None:intergenic 45.0%
TTCCTTAACCCGGTTCCATT+CGG - chr3_2:59316448-59316467 None:intergenic 45.0%
CTGATTCAAAGTGGTTCAGG+TGG + chr3_2:59316469-59316488 Msa0414250:CDS 45.0%
!!! CATCCATATTTTAGCCCCGA+TGG + chr3_2:59316502-59316521 Msa0414250:CDS 45.0%
ACACAAGACTCTTTCCATCG+GGG - chr3_2:59316519-59316538 None:intergenic 45.0%
ATATTCACCGTGAGGTTGGA+TGG + chr3_2:59316610-59316629 Msa0414250:CDS 45.0%
ATATTTGGGTGACCAAGCAG+GGG - chr3_2:59316680-59316699 None:intergenic 45.0%
TCAAGCCTGTGAATGTTGAG+AGG + chr3_2:59316701-59316720 Msa0414250:CDS 45.0%
TTAGGCCTCTCAACATTCAC+AGG - chr3_2:59316709-59316728 None:intergenic 45.0%
TGTGAATGTTGAGAGGCCTA+AGG + chr3_2:59316708-59316727 Msa0414250:CDS 45.0%
GTGAATGTTGAGAGGCCTAA+GGG + chr3_2:59316709-59316728 Msa0414250:CDS 45.0%
GAACAATAAGGTCCACCCTT+AGG - chr3_2:59316727-59316746 None:intergenic 45.0%
TTGGCATGTTGTTGAGCCAA+TGG - chr3_2:59316762-59316781 None:intergenic 45.0%
!! AAATCATTAAATTAAATTAA+TGG - chr3_2:59314582-59314601 None:intergenic 5.0%
ACTACCGTCAAACCTTCCCA+TGG + chr3_2:59313628-59313647 Msa0414250:CDS 50.0%
! GGAATGCTGTTGGTAGGAGA+TGG - chr3_2:59314509-59314528 None:intergenic 50.0%
GGCAAAGGAATGCTGTTGGT+AGG - chr3_2:59314515-59314534 None:intergenic 50.0%
CATATGGGAGCTTGAGCAAC+TGG + chr3_2:59314705-59314724 Msa0414250:CDS 50.0%
CATCCAGCACACTATGGGTT+TGG - chr3_2:59314879-59314898 None:intergenic 50.0%
AAACCAAGTGTCACCAGCGA+CGG + chr3_2:59315203-59315222 Msa0414250:CDS 50.0%
AATCTACGTGTCAACGCACG+AGG + chr3_2:59315235-59315254 Msa0414250:CDS 50.0%
CTAAAATCAGCGACGCCGTA+GGG - chr3_2:59315338-59315357 None:intergenic 50.0%
TTTCCGGTAGGAGATACAGC+GGG - chr3_2:59315362-59315381 None:intergenic 50.0%
! ACTGCTGTCCATTTTCCGGT+AGG - chr3_2:59315374-59315393 None:intergenic 50.0%
! AGCGACTGCTGTCCATTTTC+CGG - chr3_2:59315378-59315397 None:intergenic 50.0%
GTCGCTTCCTATGGTGGTAA+AGG + chr3_2:59315392-59315411 Msa0414250:CDS 50.0%
GGACCAACCTTTACCACCAT+AGG - chr3_2:59315402-59315421 None:intergenic 50.0%
TTTCATCGGAGAGGGAACGA+CGG + chr3_2:59315542-59315561 Msa0414250:CDS 50.0%
CATCGAACGAGTTACACCAC+CGG + chr3_2:59315619-59315638 Msa0414250:CDS 50.0%
ATCGAACGAGTTACACCACC+GGG + chr3_2:59315620-59315639 Msa0414250:CDS 50.0%
TTTACACCGGCAACTTCACC+TGG + chr3_2:59315653-59315672 Msa0414250:CDS 50.0%
GGGTTGAGATGGTGAGCATT+GGG - chr3_2:59315791-59315810 None:intergenic 50.0%
!! GCGAGATAAACGGGTTGAGA+TGG - chr3_2:59315802-59315821 None:intergenic 50.0%
TATCTCGCCTGATTCAACGC+GGG + chr3_2:59315814-59315833 Msa0414250:CDS 50.0%
CTAACTCTCTTCCGGTTCAC+CGG + chr3_2:59315920-59315939 Msa0414250:CDS 50.0%
AAGGAAACGAACCGGTGAAC+CGG - chr3_2:59315934-59315953 None:intergenic 50.0%
GTAAGCGATTCTGTCTCCAG+AGG - chr3_2:59315966-59315985 None:intergenic 50.0%
CGGTTCACTACGTAAACACC+AGG - chr3_2:59315998-59316017 None:intergenic 50.0%
GTTTACGTAGTGAACCGGGA+CGG + chr3_2:59316001-59316020 Msa0414250:CDS 50.0%
!! TTTTTCGGTTCGAACCGTCC+CGG - chr3_2:59316018-59316037 None:intergenic 50.0%
GGACGGTTCGAACCGAAAAA+AGG + chr3_2:59316018-59316037 Msa0414250:CDS 50.0%
CAATGGCTTTCTCAACCGCA+TGG + chr3_2:59316050-59316069 Msa0414250:CDS 50.0%
! ATCCATTTTCCGTCTGGCGA+TGG - chr3_2:59316223-59316242 None:intergenic 50.0%
! GTTTTCCGTTCTGGTCGATC+AGG + chr3_2:59316244-59316263 Msa0414250:CDS 50.0%
GCCGTTCACGGAGAGAAAAA+CGG + chr3_2:59316292-59316311 Msa0414250:CDS 50.0%
!! CCCGTTTTTCTCTCCGTGAA+CGG - chr3_2:59316296-59316315 None:intergenic 50.0%
CCGTTCACGGAGAGAAAAAC+GGG + chr3_2:59316293-59316312 Msa0414250:CDS 50.0%
GGTCGGATACAATGTGCAAC+TGG + chr3_2:59316341-59316360 Msa0414250:CDS 50.0%
AGCGATCCATTCTCCATCAG+GGG - chr3_2:59316368-59316387 None:intergenic 50.0%
TCTCAAAGCTTCCACTTCCC+GGG - chr3_2:59316411-59316430 None:intergenic 50.0%
TTGATCCACCCGAATGGAAC+CGG + chr3_2:59316436-59316455 Msa0414250:CDS 50.0%
TCCTTAACCCGGTTCCATTC+GGG - chr3_2:59316447-59316466 None:intergenic 50.0%
TTCAAAGTGGTTCAGGTGGG+AGG + chr3_2:59316473-59316492 Msa0414250:CDS 50.0%
ACCTGAACCATCCAACCTCA+CGG - chr3_2:59316620-59316639 None:intergenic 50.0%
ATGAAGATGGAACCCCTGCT+TGG + chr3_2:59316665-59316684 Msa0414250:CDS 50.0%
AATGTTGAGAGGCCTAAGGG+TGG + chr3_2:59316712-59316731 Msa0414250:CDS 50.0%
ACAGGGCTACATGCACAATG+TGG + chr3_2:59316796-59316815 Msa0414250:CDS 50.0%
CCAGCACACTATGGGTTTGG+AGG - chr3_2:59314876-59314895 None:intergenic 55.0%
CCTCCAAACCCATAGTGTGC+TGG + chr3_2:59314873-59314892 Msa0414250:CDS 55.0%
ACCCGAAGACTCACTCCCTA+CGG + chr3_2:59315320-59315339 Msa0414250:CDS 55.0%
GCTAAAATCAGCGACGCCGT+AGG - chr3_2:59315339-59315358 None:intergenic 55.0%
TTCCGGTAGGAGATACAGCG+GGG - chr3_2:59315361-59315380 None:intergenic 55.0%
TGGACAGCAGTCGCTTCCTA+TGG + chr3_2:59315383-59315402 Msa0414250:CDS 55.0%
ACAGCAGTCGCTTCCTATGG+TGG + chr3_2:59315386-59315405 Msa0414250:CDS 55.0%
AGTGAGCTCCTCTACTTCGC+CGG - chr3_2:59315423-59315442 None:intergenic 55.0%
TCACTCACGACGGAACTCAC+CGG + chr3_2:59315462-59315481 Msa0414250:CDS 55.0%
AAGTAGTTGAGCACGGTGGC+TGG + chr3_2:59315489-59315508 Msa0414250:CDS 55.0%
GGTGTAAACGCGCGTAAACC+CGG - chr3_2:59315641-59315660 None:intergenic 55.0%
GGGTTTACGCGCGTTTACAC+CGG + chr3_2:59315640-59315659 Msa0414250:CDS 55.0%
! ATGTGACGGAAGCTAGAGCC+AGG - chr3_2:59315713-59315732 None:intergenic 55.0%
! CGGGTTGAGATGGTGAGCAT+TGG - chr3_2:59315792-59315811 None:intergenic 55.0%
GTCGGGTATCACAAGTGCAG+AGG + chr3_2:59315836-59315855 Msa0414250:CDS 55.0%
CTTTCTCAACCGCATGGGAC+CGG + chr3_2:59316056-59316075 Msa0414250:CDS 55.0%
TTTCTCAACCGCATGGGACC+GGG + chr3_2:59316057-59316076 Msa0414250:CDS 55.0%
TTTCCGTCACCATCGCCAGA+CGG + chr3_2:59316211-59316230 Msa0414250:CDS 55.0%
TTTCCGTCTGGCGATGGTGA+CGG - chr3_2:59316217-59316236 None:intergenic 55.0%
CACCATCGCCAGACGGAAAA+TGG + chr3_2:59316218-59316237 Msa0414250:CDS 55.0%
ATGTGCAACTGGTCCCCTGA+TGG + chr3_2:59316352-59316371 Msa0414250:CDS 55.0%
ACTGGTCCCCTGATGGAGAA+TGG + chr3_2:59316359-59316378 Msa0414250:CDS 55.0%
TGCATCCGATCGTCATGACC+CGG + chr3_2:59316390-59316409 Msa0414250:CDS 55.0%
ACTTCCCGGGTCATGACGAT+CGG - chr3_2:59316398-59316417 None:intergenic 55.0%
TGATCCACCCGAATGGAACC+GGG + chr3_2:59316437-59316456 Msa0414250:CDS 55.0%
TTAACCCGGTTCCATTCGGG+TGG - chr3_2:59316444-59316463 None:intergenic 55.0%
ACCCGAATGGAACCGGGTTA+AGG + chr3_2:59316443-59316462 Msa0414250:CDS 55.0%
ACCGTGAGGTTGGATGGTTC+AGG + chr3_2:59316616-59316635 Msa0414250:CDS 55.0%
TCACCGTCGCTGGTGACACT+TGG - chr3_2:59315209-59315228 None:intergenic 60.0%
GTGTCAACGCACGAGGATCC+CGG + chr3_2:59315242-59315261 Msa0414250:CDS 60.0%
CAGCTGACACGTGGTACACC+GGG - chr3_2:59315263-59315282 None:intergenic 60.0%
CCAGCTGACACGTGGTACAC+CGG - chr3_2:59315264-59315283 None:intergenic 60.0%
GCCGTAGGGAGTGAGTCTTC+GGG - chr3_2:59315324-59315343 None:intergenic 60.0%
CGCCGTAGGGAGTGAGTCTT+CGG - chr3_2:59315325-59315344 None:intergenic 60.0%
AGCCCCGCTGTATCTCCTAC+CGG + chr3_2:59315356-59315375 Msa0414250:CDS 60.0%
! AGGTTGGTCCGGCGAAGTAG+AGG + chr3_2:59315412-59315431 Msa0414250:CDS 60.0%
GGTGAGTTCCGTCGTGAGTG+AGG - chr3_2:59315463-59315482 None:intergenic 60.0%
GTGCGATCGTCCACCCAACT+TGG - chr3_2:59315515-59315534 None:intergenic 60.0%
ATCGGAGAGGGAACGACGGT+TGG + chr3_2:59315546-59315565 Msa0414250:CDS 60.0%
GTAAACGCGCGTAAACCCGG+TGG - chr3_2:59315638-59315657 None:intergenic 60.0%
GCCAGGTCTTCTCGTTGCGA+CGG - chr3_2:59315696-59315715 None:intergenic 60.0%
TCGCCTGATTCAACGCGGGT+CGG + chr3_2:59315818-59315837 Msa0414250:CDS 60.0%
TACCCGACCCGCGTTGAATC+AGG - chr3_2:59315824-59315843 None:intergenic 60.0%
!! GTCTGGCGATGGTGACGGAA+AGG - chr3_2:59316212-59316231 None:intergenic 60.0%
GCATCCGATCGTCATGACCC+GGG + chr3_2:59316391-59316410 Msa0414250:CDS 60.0%
GATCGTCATGACCCGGGAAG+TGG + chr3_2:59316397-59316416 Msa0414250:CDS 60.0%
CCGGTGTACCACGTGTCAGC+TGG + chr3_2:59315261-59315280 Msa0414250:CDS 65.0%
ACGGCAGTCCAGCTGACACG+TGG - chr3_2:59315272-59315291 None:intergenic 65.0%
! AGCACGGTGGCTGGCCAAGT+TGG + chr3_2:59315498-59315517 Msa0414250:CDS 65.0%
! GCACGGTGGCTGGCCAAGTT+GGG + chr3_2:59315499-59315518 Msa0414250:CDS 65.0%
GCCGTCGCAACGAGAAGACC+TGG + chr3_2:59315692-59315711 Msa0414250:CDS 65.0%
CGCCTGATTCAACGCGGGTC+GGG + chr3_2:59315819-59315838 Msa0414250:CDS 65.0%
AGGTCGAACCCGGTCCCATG+CGG - chr3_2:59316068-59316087 None:intergenic 65.0%
! CGGTGGCTGGCCAAGTTGGG+TGG + chr3_2:59315502-59315521 Msa0414250:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 59313523 59316825 59313523 ID=Msa0414250;Name=Msa0414250
chr3_2 mRNA 59313523 59316825 59313523 ID=Msa0414250-mRNA-1;Parent=Msa0414250;Name=Msa0414250-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.6|0.33|1|1|1|6|0|836
chr3_2 exon 59313523 59313657 59313523 ID=Msa0414250-mRNA-1:exon:12357;Parent=Msa0414250-mRNA-1
chr3_2 exon 59314005 59314085 59314005 ID=Msa0414250-mRNA-1:exon:12358;Parent=Msa0414250-mRNA-1
chr3_2 exon 59314268 59314325 59314268 ID=Msa0414250-mRNA-1:exon:12359;Parent=Msa0414250-mRNA-1
chr3_2 exon 59314414 59314569 59314414 ID=Msa0414250-mRNA-1:exon:12360;Parent=Msa0414250-mRNA-1
chr3_2 exon 59314644 59315079 59314644 ID=Msa0414250-mRNA-1:exon:12361;Parent=Msa0414250-mRNA-1
chr3_2 exon 59315181 59316825 59315181 ID=Msa0414250-mRNA-1:exon:12362;Parent=Msa0414250-mRNA-1
chr3_2 CDS 59313523 59313657 59313523 ID=Msa0414250-mRNA-1:cds;Parent=Msa0414250-mRNA-1
chr3_2 CDS 59314005 59314085 59314005 ID=Msa0414250-mRNA-1:cds;Parent=Msa0414250-mRNA-1
chr3_2 CDS 59314268 59314325 59314268 ID=Msa0414250-mRNA-1:cds;Parent=Msa0414250-mRNA-1
chr3_2 CDS 59314414 59314569 59314414 ID=Msa0414250-mRNA-1:cds;Parent=Msa0414250-mRNA-1
chr3_2 CDS 59314644 59315079 59314644 ID=Msa0414250-mRNA-1:cds;Parent=Msa0414250-mRNA-1
chr3_2 CDS 59315181 59316825 59315181 ID=Msa0414250-mRNA-1:cds;Parent=Msa0414250-mRNA-1
Gene Sequence

>Msa0414250

ATGGATCAACATCATATTTCTTTCTTGTTAGAGGAGTTCATAAATGAACAATCAATGGATCATCAATATTTGATGAATCAACAACATTCACTTGAAGAAACAGATACTACCGTCAAACCTTCCCATGGAAATAATCGAGTTGAAGAAGGAAATACATTAACTAATGCTGATGCAGCATCACCCTCAGCTCATTTAGAATTGGTCAGTTCGTCACAGGAACAAGATGTTGATGTCAGAGGCTTTCAGAAAATTACCAAGACTAATAATGACCAATTTTCTCTAAATCACAATGCTTTCATTCAATTAAGCTCAATTATTGAGGAACAATTTCTTGAGGATGATGATTTTAGAGATTCAAAATCTAAGCCATCTCCTACCAACAGCATTCCTTTGCCTCTTACATCTCAAACACTTCCTATGCCTTTTGAAGGTGAGGTTAATGTATTAAACCCTTCTGAAATAGTGGAAAATTCAAGTTCTCCTTGTATTGTCATATGGGAGCTTGAGCAACTGGTCTCCGAGAATTATTTGGATTACGAGAACTTGTCTTTGTTGACTGATTTCTTTGTTAAGTATCCATCAGTTCTTTTAAGGGGCACTTCACTGAGTACTAGATACAAGTGTTATGCATACACTTGTCTAGCTGAGCTATTACAATTCCTCCAAACCCATAGTGTGCTGGATGTGTTAGGTTCAAGTCACTCTGAATTCGTTAAGCTACTACACAATTTGCGCAGCTTTGGTTTTGATAAGGATTGGTTGGATAGTGTTGAAAGACGTGCTCTGTTTTCGGATATAAAAGTCTCTCAAGATGCATTGCAAAAACTGTTGGATTCTAAGCAACAAGTCTCCAAAGAAGTTGAAGTACAATATTCAAGGATCAAAGATAAACCAAGTGTCACCAGCGACGGTGAGTATTTAATCTACGTGTCAACGCACGAGGATCCCGGTGTACCACGTGTCAGCTGGACTGCCGTCTATTCCACCCATCTCAAATCTGGTAACACCCGAAGACTCACTCCCTACGGCGTCGCTGATTTTAGCCCCGCTGTATCTCCTACCGGAAAATGGACAGCAGTCGCTTCCTATGGTGGTAAAGGTTGGTCCGGCGAAGTAGAGGAGCTCACTGCAGACATCTACGTTTTCCTCACTCACGACGGAACTCACCGGTACAAAGTAGTTGAGCACGGTGGCTGGCCAAGTTGGGTGGACGATCGCACTATTTACTTTCATCGGAGAGGGAACGACGGTTGGTTGAGTATTTACAGAGCAATTCTCACCACTGATTCTTCTAACTCTGTTCTCATCGAACGAGTTACACCACCGGGTTTACGCGCGTTTACACCGGCAACTTCACCTGGAAACAACAAATTCATCGCCGTCGCAACGAGAAGACCTGGCTCTAGCTTCCGTCACATCGAATTGTTCGACCTGGTTAACAATGAATTCAAGGAGCTAACTCGTTTTGTCTCTCCCAATGCTCACCATCTCAACCCGTTTATCTCGCCTGATTCAACGCGGGTCGGGTATCACAAGTGCAGAGGAAAAGAAAGTAACACCAGTCCTCAGTTTATGCTAGAGAATGTTCGCAGCCCCGTTCCAAATCTAACTCTCTTCCGGTTCACCGGTTCGTTTCCTTTTTTTTCACCCTCTGGAGACAGAATCGCTTACGTTGAAATGCCTGGTGTTTACGTAGTGAACCGGGACGGTTCGAACCGAAAAAAGGTTTCTGATGCAATGGCTTTCTCAACCGCATGGGACCGGGTTCGACCTAGTGTAATCTACACAGCAGTTGGAGAAACCTTTGCTTCAGAAAGCACTGAGGTTGATATCATATCCATCCATGTTGACCGAAATTTCGTCAAGAAGTTGACCCTAGACGGAAAGAACAATGCCTTTCCGTCACCATCGCCAGACGGAAAATGGATTGTTTTCCGTTCTGGTCGATCAGGTCATAAGAATCTTTACTTAATGGATGCCGTTCACGGAGAGAAAAACGGGCTTAAGAGATTAACAGAGGGTCCATGGTCGGATACAATGTGCAACTGGTCCCCTGATGGAGAATGGATCGCTTTTGCATCCGATCGTCATGACCCGGGAAGTGGAAGCTTTGAGATTTATTTGATCCACCCGAATGGAACCGGGTTAAGGAAACTGATTCAAAGTGGTTCAGGTGGGAGGACGAATCATCCATATTTTAGCCCCGATGGAAAGAGTCTTGTGTTTACGTCAGATTATGCAGGTATATCAGCTGAGCCTATCTCTAACCCGCATCATTATCAACCTTATGGTGAAATATTCACCGTGAGGTTGGATGGTTCAGGTTTAACGAGATTGACTCATAATTCTTATGAAGATGGAACCCCTGCTTGGTCACCCAAATATATCAAGCCTGTGAATGTTGAGAGGCCTAAGGGTGGACCTTATTGTTCATTTGAGGATTGCCATTGGCTCAACAACATGCCAAACTACATTGTTAGTACAGGGCTACATGCACAATGTGGTCTGTAA
Protein sequence

>Msa0414250

MDQHHISFLLEEFINEQSMDHQYLMNQQHSLEETDTTVKPSHGNNRVEEGNTLTNADAASPSAHLELVSSSQEQDVDVRGFQKITKTNNDQFSLNHNAFIQLSSIIEEQFLEDDDFRDSKSKPSPTNSIPLPLTSQTLPMPFEGEVNVLNPSEIVENSSSPCIVIWELEQLVSENYLDYENLSLLTDFFVKYPSVLLRGTSLSTRYKCYAYTCLAELLQFLQTHSVLDVLGSSHSEFVKLLHNLRSFGFDKDWLDSVERRALFSDIKVSQDALQKLLDSKQQVSKEVEVQYSRIKDKPSVTSDGEYLIYVSTHEDPGVPRVSWTAVYSTHLKSGNTRRLTPYGVADFSPAVSPTGKWTAVASYGGKGWSGEVEELTADIYVFLTHDGTHRYKVVEHGGWPSWVDDRTIYFHRRGNDGWLSIYRAILTTDSSNSVLIERVTPPGLRAFTPATSPGNNKFIAVATRRPGSSFRHIELFDLVNNEFKELTRFVSPNAHHLNPFISPDSTRVGYHKCRGKESNTSPQFMLENVRSPVPNLTLFRFTGSFPFFSPSGDRIAYVEMPGVYVVNRDGSNRKKVSDAMAFSTAWDRVRPSVIYTAVGETFASESTEVDIISIHVDRNFVKKLTLDGKNNAFPSPSPDGKWIVFRSGRSGHKNLYLMDAVHGEKNGLKRLTEGPWSDTMCNWSPDGEWIAFASDRHDPGSGSFEIYLIHPNGTGLRKLIQSGSGGRTNHPYFSPDGKSLVFTSDYAGISAEPISNPHHYQPYGEIFTVRLDGSGLTRLTHNSYEDGTPAWSPKYIKPVNVERPKGGPYCSFEDCHWLNNMPNYIVSTGLHAQCGL*