Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0434150 | AES73890.2 | 92.893 | 197 | 11 | 2 | 1 | 197 | 1 | 194 | 1.72e-125 | 362 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0434150 | G7J3I3 | 92.893 | 197 | 11 | 2 | 1 | 197 | 1 | 194 | 8.21e-126 | 362 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0383770 | Msa0434150 | 0.916680 | 1.330921e-85 | -8.615850e-47 |
| Msa0423220 | Msa0434150 | 0.802481 | 5.496609e-49 | -8.615850e-47 |
| Msa0434150 | Msa0565700 | 0.810764 | 9.802117e-51 | -8.615850e-47 |
| Msa0434150 | Msa0620340 | 0.813495 | 2.487331e-51 | -8.615850e-47 |
| Msa0434150 | Msa0647300 | 0.817420 | 3.328933e-52 | -8.615850e-47 |
| Msa0434150 | Msa0686830 | 0.819440 | 1.160343e-52 | -8.615850e-47 |
| Msa0434150 | Msa0701660 | 0.829032 | 6.456385e-55 | -8.615850e-47 |
| Msa0434150 | Msa0970270 | 0.800117 | 1.674923e-48 | -8.615850e-47 |
| Msa0434150 | Msa1108560 | 0.803511 | 3.367079e-49 | -8.615850e-47 |
| Msa0434150 | Msa1165460 | 0.804430 | 2.168335e-49 | -8.615850e-47 |
| Msa0434150 | Msa1263110 | 0.801307 | 9.575995e-49 | -8.615850e-47 |
| Msa0434150 | Msa1322500 | 0.804505 | 2.092102e-49 | -8.615850e-47 |
| Msa0434150 | Msa1361980 | 0.803395 | 3.557426e-49 | -8.615850e-47 |
| Msa0434150 | Msa1380790 | 0.811796 | 5.851473e-51 | -8.615850e-47 |
| Msa0434150 | Msa1455360 | 0.815487 | 9.014762e-52 | -8.615850e-47 |
| Msa0354550 | Msa0434150 | 0.802772 | 4.786483e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0434150 | MtrunA17_Chr3g0140411 | 92.893 | 197 | 11 | 2 | 1 | 197 | 9 | 202 | 2.10e-129 | 362 |
| Msa0434150 | MtrunA17_Chr3g0140421 | 83.246 | 191 | 28 | 3 | 1 | 191 | 1 | 187 | 9.17e-115 | 324 |
| Msa0434150 | MtrunA17_Chr8g0340431 | 26.702 | 191 | 103 | 6 | 21 | 180 | 1 | 185 | 2.49e-13 | 65.5 |
| Msa0434150 | MtrunA17_Chr8g0340461 | 26.804 | 194 | 105 | 7 | 21 | 182 | 1 | 189 | 1.92e-12 | 63.2 |
| Msa0434150 | MtrunA17_Chr8g0340451 | 26.289 | 194 | 106 | 7 | 21 | 182 | 1 | 189 | 1.79e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0434150 | AT4G32860.1 | 33.971 | 209 | 99 | 8 | 1 | 185 | 1 | 194 | 8.43e-23 | 91.3 |
Find 43 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACGGCACACCTTCATTTGC+CGG | 0.291928 | 3_2:-86910611 | None:intergenic |
| GTAATATCTGTAACATTGTT+TGG | 0.307750 | 3_2:-86910252 | None:intergenic |
| TAATATCTGTAACATTGTTT+GGG | 0.386224 | 3_2:-86910251 | None:intergenic |
| GAGAGCTTAGGTTGCAGAAA+TGG | 0.410450 | 3_2:+86910748 | Msa0434150:CDS |
| TTGCCGGCGACCTTCCTCCT+TGG | 0.425099 | 3_2:-86910595 | None:intergenic |
| AGCAAAACGGTGCGTTTACT+AGG | 0.432952 | 3_2:+86910315 | Msa0434150:CDS |
| TCCTTTCATTGCTGCTTCCA+CGG | 0.447597 | 3_2:-86910629 | None:intergenic |
| GTCGGCACGTGAGTGCTTCA+TGG | 0.449621 | 3_2:-86910452 | None:intergenic |
| TATGTATTTCATTTCACCGT+TGG | 0.457166 | 3_2:+86910488 | Msa0434150:CDS |
| CGGCGACCTTCCTCCTTGGC+CGG | 0.461416 | 3_2:-86910591 | None:intergenic |
| CTATGAACTCCTCCGCCGCC+TGG | 0.473317 | 3_2:-86910713 | None:intergenic |
| TCATGTTGACCAGGCGGCGG+AGG | 0.488533 | 3_2:+86910704 | Msa0434150:CDS |
| AAGCTTCCTCCTACTACTGC+TGG | 0.509632 | 3_2:-86910562 | None:intergenic |
| GGAAGGTCGCCGGCAAATGA+AGG | 0.527011 | 3_2:+86910602 | Msa0434150:CDS |
| TTGTCTCCGGCCAAGGAGGA+AGG | 0.528048 | 3_2:+86910585 | Msa0434150:CDS |
| GCTTAGGTTGCAGAAATGGT+TGG | 0.533031 | 3_2:+86910752 | Msa0434150:CDS |
| CTAGGTTTCATGTTACAAAA+CGG | 0.539359 | 3_2:+86910333 | Msa0434150:CDS |
| ATGCAAGAAATGATGAAGAG+AGG | 0.551359 | 3_2:+86910387 | Msa0434150:CDS |
| GCGGTGGCGCCAGCAGTAGT+AGG | 0.554764 | 3_2:+86910553 | Msa0434150:CDS |
| GTTTCATGTTGACCAGGCGG+CGG | 0.558520 | 3_2:+86910701 | Msa0434150:CDS |
| GAAAGAGTTTCATGTTGACC+AGG | 0.578595 | 3_2:+86910695 | Msa0434150:CDS |
| CGGCCAAGGAGGAAGGTCGC+CGG | 0.581708 | 3_2:+86910592 | Msa0434150:CDS |
| TGTTACAGATATTACATGCA+TGG | 0.585321 | 3_2:+86910260 | Msa0434150:CDS |
| TGAAGATGACAACTTTGTCA+AGG | 0.599361 | 3_2:+86910658 | Msa0434150:CDS |
| TTTGTCAAGGGAGACTGAGA+AGG | 0.604036 | 3_2:+86910671 | Msa0434150:CDS |
| GTGGCGCCAGCAGTAGTAGG+AGG | 0.604984 | 3_2:+86910556 | Msa0434150:CDS |
| TTTCAATTACCACCGTTGGT+TGG | 0.610216 | 3_2:-86910287 | None:intergenic |
| GCCGTGGAAGCAGCAATGAA+AGG | 0.610528 | 3_2:+86910628 | Msa0434150:CDS |
| GCGCCACCGCGAGAGAGACG+CGG | 0.622064 | 3_2:-86910540 | None:intergenic |
| GAAGATGACAACTTTGTCAA+GGG | 0.623449 | 3_2:+86910659 | Msa0434150:CDS |
| ACATATCAGCATCACGTGGT+CGG | 0.624919 | 3_2:-86910470 | None:intergenic |
| CGGCAAATGAAGGTGTGCCG+TGG | 0.631422 | 3_2:+86910612 | Msa0434150:CDS |
| AGTCCACCGCGTCTCTCTCG+CGG | 0.632836 | 3_2:+86910534 | Msa0434150:CDS |
| GTGGAAGCAGCAATGAAAGG+AGG | 0.655270 | 3_2:+86910631 | Msa0434150:CDS |
| CAGCTGAACTGATACTCCAA+CGG | 0.655898 | 3_2:-86910504 | None:intergenic |
| CCACCGCGTCTCTCTCGCGG+TGG | 0.660075 | 3_2:+86910537 | Msa0434150:CDS |
| ATGCATGGATAACCAACCAA+CGG | 0.673857 | 3_2:+86910275 | Msa0434150:CDS |
| AGAGTTTCATGTTGACCAGG+CGG | 0.681822 | 3_2:+86910698 | Msa0434150:CDS |
| GAAAACACTGAACAACGTGA+TGG | 0.683073 | 3_2:+86910422 | Msa0434150:CDS |
| CATGGATAACCAACCAACGG+TGG | 0.704369 | 3_2:+86910278 | Msa0434150:CDS |
| CCACCGCGAGAGAGACGCGG+TGG | 0.712456 | 3_2:-86910537 | None:intergenic |
| AAATACATATCAGCATCACG+TGG | 0.752599 | 3_2:-86910474 | None:intergenic |
| AACTTCATACAGAAACAGAG+TGG | 0.756819 | 3_2:+86910223 | Msa0434150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATATCTGTAACATTGTTT+GGG | - | chr3_2:86910254-86910273 | None:intergenic | 20.0% |
| ! | GTAATATCTGTAACATTGTT+TGG | - | chr3_2:86910255-86910274 | None:intergenic | 25.0% |
| ! | AATTGAAAAAGTGAGCAAAA+CGG | + | chr3_2:86910302-86910321 | Msa0434150:CDS | 25.0% |
| TGTTACAGATATTACATGCA+TGG | + | chr3_2:86910260-86910279 | Msa0434150:CDS | 30.0% | |
| CTAGGTTTCATGTTACAAAA+CGG | + | chr3_2:86910333-86910352 | Msa0434150:CDS | 30.0% | |
| ATGAAGAGAGGAAAAAACAT+AGG | + | chr3_2:86910399-86910418 | Msa0434150:CDS | 30.0% | |
| TGAAGAGAGGAAAAAACATA+GGG | + | chr3_2:86910400-86910419 | Msa0434150:CDS | 30.0% | |
| TATGTATTTCATTTCACCGT+TGG | + | chr3_2:86910488-86910507 | Msa0434150:CDS | 30.0% | |
| AACTTCATACAGAAACAGAG+TGG | + | chr3_2:86910223-86910242 | Msa0434150:CDS | 35.0% | |
| !!! | ACTTTTTCAATTACCACCGT+TGG | - | chr3_2:86910294-86910313 | None:intergenic | 35.0% |
| ATGCAAGAAATGATGAAGAG+AGG | + | chr3_2:86910387-86910406 | Msa0434150:CDS | 35.0% | |
| AAATACATATCAGCATCACG+TGG | - | chr3_2:86910477-86910496 | None:intergenic | 35.0% | |
| TGAAGATGACAACTTTGTCA+AGG | + | chr3_2:86910658-86910677 | Msa0434150:CDS | 35.0% | |
| GAAGATGACAACTTTGTCAA+GGG | + | chr3_2:86910659-86910678 | Msa0434150:CDS | 35.0% | |
| ! | ACAAGTTTTACAGAGAGCTT+AGG | + | chr3_2:86910736-86910755 | Msa0434150:CDS | 35.0% |
| ATGCATGGATAACCAACCAA+CGG | + | chr3_2:86910275-86910294 | Msa0434150:CDS | 40.0% | |
| TTTCAATTACCACCGTTGGT+TGG | - | chr3_2:86910290-86910309 | None:intergenic | 40.0% | |
| GAAAACACTGAACAACGTGA+TGG | + | chr3_2:86910422-86910441 | Msa0434150:CDS | 40.0% | |
| GAAAGAGTTTCATGTTGACC+AGG | + | chr3_2:86910695-86910714 | Msa0434150:CDS | 40.0% | |
| AGCAAAACGGTGCGTTTACT+AGG | + | chr3_2:86910315-86910334 | Msa0434150:CDS | 45.0% | |
| ACATATCAGCATCACGTGGT+CGG | - | chr3_2:86910473-86910492 | None:intergenic | 45.0% | |
| CAGCTGAACTGATACTCCAA+CGG | - | chr3_2:86910507-86910526 | None:intergenic | 45.0% | |
| ! | TAGGAGGAAGCTTTTGTCTC+CGG | + | chr3_2:86910572-86910591 | Msa0434150:CDS | 45.0% |
| ! | TCCTTTCATTGCTGCTTCCA+CGG | - | chr3_2:86910632-86910651 | None:intergenic | 45.0% |
| TTTGTCAAGGGAGACTGAGA+AGG | + | chr3_2:86910671-86910690 | Msa0434150:CDS | 45.0% | |
| AGAGTTTCATGTTGACCAGG+CGG | + | chr3_2:86910698-86910717 | Msa0434150:CDS | 45.0% | |
| ! | GAGAGCTTAGGTTGCAGAAA+TGG | + | chr3_2:86910748-86910767 | Msa0434150:CDS | 45.0% |
| ! | GCTTAGGTTGCAGAAATGGT+TGG | + | chr3_2:86910752-86910771 | Msa0434150:CDS | 45.0% |
| CATGGATAACCAACCAACGG+TGG | + | chr3_2:86910278-86910297 | Msa0434150:CDS | 50.0% | |
| AAGCTTCCTCCTACTACTGC+TGG | - | chr3_2:86910565-86910584 | None:intergenic | 50.0% | |
| GTGGAAGCAGCAATGAAAGG+AGG | + | chr3_2:86910631-86910650 | Msa0434150:CDS | 50.0% | |
| ! | GAAGCTTTTGTCTCCGGCCA+AGG | + | chr3_2:86910578-86910597 | Msa0434150:CDS | 55.0% |
| CACGGCACACCTTCATTTGC+CGG | - | chr3_2:86910614-86910633 | None:intergenic | 55.0% | |
| GCCGTGGAAGCAGCAATGAA+AGG | + | chr3_2:86910628-86910647 | Msa0434150:CDS | 55.0% | |
| GTTTCATGTTGACCAGGCGG+CGG | + | chr3_2:86910701-86910720 | Msa0434150:CDS | 55.0% | |
| GTCGGCACGTGAGTGCTTCA+TGG | - | chr3_2:86910455-86910474 | None:intergenic | 60.0% | |
| ! | GCTTTTGTCTCCGGCCAAGG+AGG | + | chr3_2:86910581-86910600 | Msa0434150:CDS | 60.0% |
| TTGTCTCCGGCCAAGGAGGA+AGG | + | chr3_2:86910585-86910604 | Msa0434150:CDS | 60.0% | |
| GGAAGGTCGCCGGCAAATGA+AGG | + | chr3_2:86910602-86910621 | Msa0434150:CDS | 60.0% | |
| ! | CGGCAAATGAAGGTGTGCCG+TGG | + | chr3_2:86910612-86910631 | Msa0434150:CDS | 60.0% |
| AGTCCACCGCGTCTCTCTCG+CGG | + | chr3_2:86910534-86910553 | Msa0434150:CDS | 65.0% | |
| GTGGCGCCAGCAGTAGTAGG+AGG | + | chr3_2:86910556-86910575 | Msa0434150:CDS | 65.0% | |
| TTGCCGGCGACCTTCCTCCT+TGG | - | chr3_2:86910598-86910617 | None:intergenic | 65.0% | |
| TCATGTTGACCAGGCGGCGG+AGG | + | chr3_2:86910704-86910723 | Msa0434150:CDS | 65.0% | |
| CTATGAACTCCTCCGCCGCC+TGG | - | chr3_2:86910716-86910735 | None:intergenic | 65.0% | |
| GCGGTGGCGCCAGCAGTAGT+AGG | + | chr3_2:86910553-86910572 | Msa0434150:CDS | 70.0% | |
| CGGCGACCTTCCTCCTTGGC+CGG | - | chr3_2:86910594-86910613 | None:intergenic | 70.0% | |
| CGGCCAAGGAGGAAGGTCGC+CGG | + | chr3_2:86910592-86910611 | Msa0434150:CDS | 70.0% | |
| CCACCGCGAGAGAGACGCGG+TGG | - | chr3_2:86910540-86910559 | None:intergenic | 75.0% | |
| CCACCGCGTCTCTCTCGCGG+TGG | + | chr3_2:86910537-86910556 | Msa0434150:CDS | 75.0% | |
| GCGCCACCGCGAGAGAGACG+CGG | - | chr3_2:86910543-86910562 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 86910219 | 86910812 | 86910219 | ID=Msa0434150;Name=Msa0434150 |
| chr3_2 | mRNA | 86910219 | 86910812 | 86910219 | ID=Msa0434150-mRNA-1;Parent=Msa0434150;Name=Msa0434150-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|197 |
| chr3_2 | exon | 86910219 | 86910812 | 86910219 | ID=Msa0434150-mRNA-1:exon:25240;Parent=Msa0434150-mRNA-1 |
| chr3_2 | CDS | 86910219 | 86910812 | 86910219 | ID=Msa0434150-mRNA-1:cds;Parent=Msa0434150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
