Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0383770 | XP_003603639.3 | 94.624 | 186 | 8 | 1 | 1 | 186 | 9 | 192 | 4.71e-127 | 366 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0383770 | A0A396J0I8 | 94.624 | 186 | 8 | 1 | 1 | 186 | 9 | 192 | 2.25e-127 | 366 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006080 | Msa0383770 | 0.819174 | 1.333575e-52 | -8.615850e-47 |
| Msa0013270 | Msa0383770 | 0.828633 | 8.066697e-55 | -8.615850e-47 |
| Msa0017710 | Msa0383770 | 0.806881 | 6.631875e-50 | -8.615850e-47 |
| Msa0024650 | Msa0383770 | 0.805614 | 1.226243e-49 | -8.615850e-47 |
| Msa0056230 | Msa0383770 | -0.808544 | 2.939374e-50 | -8.615850e-47 |
| Msa0062190 | Msa0383770 | 0.823651 | 1.234889e-53 | -8.615850e-47 |
| Msa0073110 | Msa0383770 | 0.805536 | 1.273310e-49 | -8.615850e-47 |
| Msa0074620 | Msa0383770 | 0.804559 | 2.038340e-49 | -8.615850e-47 |
| Msa0098380 | Msa0383770 | 0.802125 | 6.507812e-49 | -8.615850e-47 |
| Msa0106540 | Msa0383770 | 0.821424 | 4.067032e-53 | -8.615850e-47 |
| Msa0111780 | Msa0383770 | 0.823398 | 1.415552e-53 | -8.615850e-47 |
| Msa0121320 | Msa0383770 | 0.804855 | 1.768193e-49 | -8.615850e-47 |
| Msa0136180 | Msa0383770 | -0.818445 | 1.953360e-52 | -8.615850e-47 |
| Msa0141410 | Msa0383770 | 0.805271 | 1.447201e-49 | -8.615850e-47 |
| Msa0145050 | Msa0383770 | 0.809675 | 1.682837e-50 | -8.615850e-47 |
| Msa0170990 | Msa0383770 | 0.803068 | 4.159192e-49 | -8.615850e-47 |
| Msa0189990 | Msa0383770 | 0.807406 | 5.134885e-50 | -8.615850e-47 |
| Msa0216150 | Msa0383770 | -0.806096 | 9.712427e-50 | -8.615850e-47 |
| Msa0224870 | Msa0383770 | 0.809358 | 1.968025e-50 | -8.615850e-47 |
| Msa0364910 | Msa0383770 | -0.802322 | 5.926663e-49 | -8.615850e-47 |
| Msa0369990 | Msa0383770 | 0.800977 | 1.118707e-48 | -8.615850e-47 |
| Msa0370620 | Msa0383770 | 0.814722 | 1.332779e-51 | -8.615850e-47 |
| Msa0374040 | Msa0383770 | 0.807855 | 4.122929e-50 | -8.615850e-47 |
| Msa0374470 | Msa0383770 | 0.801205 | 1.004858e-48 | -8.615850e-47 |
| Msa0379480 | Msa0383770 | 0.800516 | 1.389206e-48 | -8.615850e-47 |
| Msa0383770 | Msa0396860 | 0.825483 | 4.572812e-54 | -8.615850e-47 |
| Msa0383770 | Msa0401420 | 0.822441 | 2.365489e-53 | -8.615850e-47 |
| Msa0383770 | Msa0404140 | 0.801092 | 1.060048e-48 | -8.615850e-47 |
| Msa0383770 | Msa0405200 | 0.816671 | 4.902720e-52 | -8.615850e-47 |
| Msa0383770 | Msa0412580 | -0.808135 | 3.592824e-50 | -8.615850e-47 |
| Msa0383770 | Msa0423200 | 0.812137 | 4.932750e-51 | -8.615850e-47 |
| Msa0383770 | Msa0423220 | 0.833104 | 6.457954e-56 | -8.615850e-47 |
| Msa0383770 | Msa0434150 | 0.916680 | 1.330921e-85 | -8.615850e-47 |
| Msa0383770 | Msa0449500 | 0.813395 | 2.615574e-51 | -8.615850e-47 |
| Msa0383770 | Msa0454160 | 0.803430 | 3.499660e-49 | -8.615850e-47 |
| Msa0383770 | Msa0457070 | -0.809492 | 1.841889e-50 | -8.615850e-47 |
| Msa0383770 | Msa0459930 | 0.809318 | 2.007523e-50 | -8.615850e-47 |
| Msa0383770 | Msa0464310 | 0.805025 | 1.629036e-49 | -8.615850e-47 |
| Msa0383770 | Msa0464620 | 0.803822 | 2.901967e-49 | -8.615850e-47 |
| Msa0383770 | Msa0477980 | 0.806708 | 7.215669e-50 | -8.615850e-47 |
| Msa0383770 | Msa0490100 | 0.819463 | 1.146332e-52 | -8.615850e-47 |
| Msa0383770 | Msa0491780 | 0.836749 | 7.795085e-57 | -8.615850e-47 |
| Msa0383770 | Msa0496070 | 0.821124 | 4.769053e-53 | -8.615850e-47 |
| Msa0383770 | Msa0497680 | 0.813411 | 2.595312e-51 | -8.615850e-47 |
| Msa0383770 | Msa0501080 | 0.818406 | 1.993284e-52 | -8.615850e-47 |
| Msa0383770 | Msa0514540 | 0.813341 | 2.688603e-51 | -8.615850e-47 |
| Msa0383770 | Msa0515130 | 0.809410 | 1.918374e-50 | -8.615850e-47 |
| Msa0383770 | Msa0535570 | 0.817580 | 3.063862e-52 | -8.615850e-47 |
| Msa0383770 | Msa0540640 | 0.808737 | 2.673451e-50 | -8.615850e-47 |
| Msa0383770 | Msa0561830 | 0.816912 | 4.329683e-52 | -8.615850e-47 |
| Msa0383770 | Msa0562050 | 0.800426 | 1.449118e-48 | -8.615850e-47 |
| Msa0383770 | Msa0565700 | 0.844946 | 5.516753e-59 | -8.615850e-47 |
| Msa0383770 | Msa0567330 | 0.825067 | 5.737449e-54 | -8.615850e-47 |
| Msa0383770 | Msa0567570 | 0.817449 | 3.278002e-52 | -8.615850e-47 |
| Msa0383770 | Msa0576320 | 0.810711 | 1.006383e-50 | -8.615850e-47 |
| Msa0383770 | Msa0583390 | 0.822274 | 2.585717e-53 | -8.615850e-47 |
| Msa0383770 | Msa0587730 | 0.804675 | 1.927458e-49 | -8.615850e-47 |
| Msa0383770 | Msa0591490 | 0.804999 | 1.649279e-49 | -8.615850e-47 |
| Msa0383770 | Msa0597170 | 0.802204 | 6.268787e-49 | -8.615850e-47 |
| Msa0383770 | Msa0606600 | 0.830432 | 2.946975e-55 | -8.615850e-47 |
| Msa0383770 | Msa0614960 | 0.817863 | 2.645171e-52 | -8.615850e-47 |
| Msa0383770 | Msa0615070 | 0.804270 | 2.341900e-49 | -8.615850e-47 |
| Msa0383770 | Msa0616010 | 0.806384 | 8.446266e-50 | -8.615850e-47 |
| Msa0383770 | Msa0618680 | 0.808399 | 3.157456e-50 | -8.615850e-47 |
| Msa0383770 | Msa0618700 | 0.824700 | 7.001765e-54 | -8.615850e-47 |
| Msa0383770 | Msa0620340 | 0.824004 | 1.020872e-53 | -8.615850e-47 |
| Msa0383770 | Msa0629160 | 0.827046 | 1.941929e-54 | -8.615850e-47 |
| Msa0383770 | Msa0631700 | 0.805338 | 1.400571e-49 | -8.615850e-47 |
| Msa0383770 | Msa0644340 | 0.804285 | 2.324499e-49 | -8.615850e-47 |
| Msa0383770 | Msa0647300 | 0.858046 | 1.077860e-62 | -8.615850e-47 |
| Msa0383770 | Msa0655370 | 0.812213 | 4.746489e-51 | -8.615850e-47 |
| Msa0383770 | Msa0655430 | 0.803498 | 3.388048e-49 | -8.615850e-47 |
| Msa0383770 | Msa0670360 | 0.806270 | 8.926664e-50 | -8.615850e-47 |
| Msa0383770 | Msa0685390 | -0.827404 | 1.593902e-54 | -8.615850e-47 |
| Msa0383770 | Msa0686830 | 0.857822 | 1.255666e-62 | -8.615850e-47 |
| Msa0383770 | Msa0699170 | 0.829089 | 6.255428e-55 | -8.615850e-47 |
| Msa0383770 | Msa0701660 | 0.827063 | 1.924139e-54 | -8.615850e-47 |
| Msa0383770 | Msa0708680 | 0.802467 | 5.533854e-49 | -8.615850e-47 |
| Msa0383770 | Msa0720870 | 0.802099 | 6.586777e-49 | -8.615850e-47 |
| Msa0383770 | Msa0731580 | 0.822088 | 2.855940e-53 | -8.615850e-47 |
| Msa0383770 | Msa0738460 | -0.800357 | 1.497308e-48 | -8.615850e-47 |
| Msa0383770 | Msa0792410 | 0.828162 | 1.048044e-54 | -8.615850e-47 |
| Msa0383770 | Msa0799120 | 0.815305 | 9.896867e-52 | -8.615850e-47 |
| Msa0383770 | Msa0802600 | 0.811990 | 5.310705e-51 | -8.615850e-47 |
| Msa0383770 | Msa0803170 | 0.814516 | 1.480986e-51 | -8.615850e-47 |
| Msa0383770 | Msa0806930 | 0.804099 | 2.541574e-49 | -8.615850e-47 |
| Msa0383770 | Msa0826240 | 0.804156 | 2.473500e-49 | -8.615850e-47 |
| Msa0383770 | Msa0829990 | 0.811663 | 6.255397e-51 | -8.615850e-47 |
| Msa0383770 | Msa0838210 | 0.810767 | 9.784598e-51 | -8.615850e-47 |
| Msa0383770 | Msa0869660 | 0.806766 | 7.015164e-50 | -8.615850e-47 |
| Msa0383770 | Msa0903020 | 0.817081 | 3.966437e-52 | -8.615850e-47 |
| Msa0383770 | Msa0932140 | 0.815829 | 7.563816e-52 | -8.615850e-47 |
| Msa0383770 | Msa0932150 | 0.806589 | 7.642502e-50 | -8.615850e-47 |
| Msa0383770 | Msa0936630 | 0.800174 | 1.631341e-48 | -8.615850e-47 |
| Msa0383770 | Msa0967900 | -0.801516 | 8.676922e-49 | -8.615850e-47 |
| Msa0383770 | Msa0970270 | 0.832932 | 7.129903e-56 | -8.615850e-47 |
| Msa0383770 | Msa0993710 | 0.803646 | 3.156228e-49 | -8.615850e-47 |
| Msa0383770 | Msa0998810 | 0.811940 | 5.443695e-51 | -8.615850e-47 |
| Msa0383770 | Msa1003730 | 0.814231 | 1.711629e-51 | -8.615850e-47 |
| Msa0383770 | Msa1003920 | 0.819366 | 1.206150e-52 | -8.615850e-47 |
| Msa0383770 | Msa1006030 | 0.816536 | 5.257522e-52 | -8.615850e-47 |
| Msa0383770 | Msa1029860 | 0.811390 | 7.170295e-51 | -8.615850e-47 |
| Msa0383770 | Msa1030320 | 0.810745 | 9.895556e-51 | -8.615850e-47 |
| Msa0383770 | Msa1035050 | 0.805826 | 1.106734e-49 | -8.615850e-47 |
| Msa0383770 | Msa1062060 | 0.818345 | 2.057855e-52 | -8.615850e-47 |
| Msa0383770 | Msa1062070 | 0.828910 | 6.913874e-55 | -8.615850e-47 |
| Msa0383770 | Msa1066150 | 0.815735 | 7.937932e-52 | -8.615850e-47 |
| Msa0383770 | Msa1075090 | 0.813100 | 3.036411e-51 | -8.615850e-47 |
| Msa0383770 | Msa1078590 | -0.806039 | 9.981268e-50 | -8.615850e-47 |
| Msa0383770 | Msa1078760 | -0.801913 | 7.193584e-49 | -8.615850e-47 |
| Msa0383770 | Msa1093190 | -0.804022 | 2.637276e-49 | -8.615850e-47 |
| Msa0383770 | Msa1094810 | 0.815850 | 7.484172e-52 | -8.615850e-47 |
| Msa0383770 | Msa1103450 | 0.826271 | 2.973420e-54 | -8.615850e-47 |
| Msa0383770 | Msa1107220 | 0.815614 | 8.447027e-52 | -8.615850e-47 |
| Msa0383770 | Msa1107330 | 0.803215 | 3.877713e-49 | -8.615850e-47 |
| Msa0383770 | Msa1108560 | 0.836836 | 7.407305e-57 | -8.615850e-47 |
| Msa0383770 | Msa1120960 | 0.804179 | 2.446157e-49 | -8.615850e-47 |
| Msa0383770 | Msa1136630 | 0.807138 | 5.852013e-50 | -8.615850e-47 |
| Msa0383770 | Msa1142590 | 0.804454 | 2.144088e-49 | -8.615850e-47 |
| Msa0383770 | Msa1146980 | 0.818915 | 1.527952e-52 | -8.615850e-47 |
| Msa0383770 | Msa1147010 | 0.827559 | 1.463324e-54 | -8.615850e-47 |
| Msa0383770 | Msa1150740 | 0.807480 | 4.950766e-50 | -8.615850e-47 |
| Msa0383770 | Msa1150750 | 0.811793 | 5.860566e-51 | -8.615850e-47 |
| Msa0383770 | Msa1150760 | 0.824563 | 7.543785e-54 | -8.615850e-47 |
| Msa0383770 | Msa1156740 | 0.804865 | 1.759502e-49 | -8.615850e-47 |
| Msa0383770 | Msa1157210 | 0.819964 | 8.807202e-53 | -8.615850e-47 |
| Msa0383770 | Msa1162430 | 0.803163 | 3.974895e-49 | -8.615850e-47 |
| Msa0383770 | Msa1165460 | 0.830334 | 3.113935e-55 | -8.615850e-47 |
| Msa0383770 | Msa1169590 | -0.802828 | 4.660777e-49 | -8.615850e-47 |
| Msa0383770 | Msa1174280 | 0.823650 | 1.235718e-53 | -8.615850e-47 |
| Msa0383770 | Msa1186340 | 0.800096 | 1.691445e-48 | -8.615850e-47 |
| Msa0383770 | Msa1186430 | 0.809959 | 1.462416e-50 | -8.615850e-47 |
| Msa0383770 | Msa1191270 | 0.800497 | 1.401764e-48 | -8.615850e-47 |
| Msa0383770 | Msa1199540 | -0.804541 | 2.055629e-49 | -8.615850e-47 |
| Msa0383770 | Msa1202110 | 0.806859 | 6.703148e-50 | -8.615850e-47 |
| Msa0383770 | Msa1204900 | -0.802586 | 5.228936e-49 | -8.615850e-47 |
| Msa0383770 | Msa1205270 | -0.817647 | 2.959089e-52 | -8.615850e-47 |
| Msa0383770 | Msa1223710 | 0.818191 | 2.229209e-52 | -8.615850e-47 |
| Msa0383770 | Msa1223960 | 0.820471 | 6.740899e-53 | -8.615850e-47 |
| Msa0383770 | Msa1223990 | 0.809724 | 1.642384e-50 | -8.615850e-47 |
| Msa0383770 | Msa1225890 | 0.821789 | 3.350132e-53 | -8.615850e-47 |
| Msa0383770 | Msa1245530 | 0.803456 | 3.456816e-49 | -8.615850e-47 |
| Msa0383770 | Msa1254870 | 0.804567 | 2.030103e-49 | -8.615850e-47 |
| Msa0383770 | Msa1263110 | 0.838619 | 2.581646e-57 | -8.615850e-47 |
| Msa0383770 | Msa1269500 | 0.806399 | 8.381401e-50 | -8.615850e-47 |
| Msa0383770 | Msa1273320 | 0.838461 | 2.835968e-57 | -8.615850e-47 |
| Msa0383770 | Msa1279200 | -0.801394 | 9.192363e-49 | -8.615850e-47 |
| Msa0383770 | Msa1281780 | 0.832604 | 8.597318e-56 | -8.615850e-47 |
| Msa0383770 | Msa1284020 | 0.809935 | 1.479753e-50 | -8.615850e-47 |
| Msa0383770 | Msa1316960 | 0.816000 | 6.926994e-52 | -8.615850e-47 |
| Msa0383770 | Msa1319190 | 0.803662 | 3.132211e-49 | -8.615850e-47 |
| Msa0383770 | Msa1320160 | 0.808092 | 3.670538e-50 | -8.615850e-47 |
| Msa0383770 | Msa1321010 | 0.802411 | 5.682796e-49 | -8.615850e-47 |
| Msa0383770 | Msa1322500 | 0.825700 | 4.062027e-54 | -8.615850e-47 |
| Msa0383770 | Msa1328430 | 0.805396 | 1.362506e-49 | -8.615850e-47 |
| Msa0383770 | Msa1361980 | 0.851969 | 6.274897e-61 | -8.615850e-47 |
| Msa0383770 | Msa1380790 | 0.823707 | 1.198452e-53 | -8.615850e-47 |
| Msa0383770 | Msa1384130 | 0.817917 | 2.570765e-52 | -8.615850e-47 |
| Msa0383770 | Msa1400290 | -0.814913 | 1.209255e-51 | -8.615850e-47 |
| Msa0383770 | Msa1405830 | 0.820627 | 6.207651e-53 | -8.615850e-47 |
| Msa0383770 | Msa1407040 | 0.802412 | 5.680489e-49 | -8.615850e-47 |
| Msa0383770 | Msa1407810 | 0.818867 | 1.566837e-52 | -8.615850e-47 |
| Msa0383770 | Msa1433360 | 0.803745 | 3.010888e-49 | -8.615850e-47 |
| Msa0383770 | Msa1437780 | 0.819869 | 9.256904e-53 | -8.615850e-47 |
| Msa0383770 | Msa1442200 | 0.803432 | 3.495829e-49 | -8.615850e-47 |
| Msa0383770 | Msa1455360 | 0.834059 | 3.729520e-56 | -8.615850e-47 |
| Msa0383770 | Msa1461660 | 0.804342 | 2.262065e-49 | -8.615850e-47 |
| Msa0383770 | Msa1462610 | 0.825592 | 4.308776e-54 | -8.615850e-47 |
| Msa0242720 | Msa0383770 | 0.806703 | 7.230480e-50 | -8.615850e-47 |
| Msa0247780 | Msa0383770 | 0.806457 | 8.150458e-50 | -8.615850e-47 |
| Msa0248320 | Msa0383770 | 0.814415 | 1.558831e-51 | -8.615850e-47 |
| Msa0270730 | Msa0383770 | -0.803005 | 4.284887e-49 | -8.615850e-47 |
| Msa0275390 | Msa0383770 | 0.801521 | 8.656881e-49 | -8.615850e-47 |
| Msa0279810 | Msa0383770 | 0.803687 | 3.095015e-49 | -8.615850e-47 |
| Msa0303900 | Msa0383770 | 0.817906 | 2.586297e-52 | -8.615850e-47 |
| Msa0307630 | Msa0383770 | 0.827363 | 1.630294e-54 | -8.615850e-47 |
| Msa0318490 | Msa0383770 | 0.814212 | 1.728625e-51 | -8.615850e-47 |
| Msa0325260 | Msa0383770 | 0.807248 | 5.546263e-50 | -8.615850e-47 |
| Msa0333770 | Msa0383770 | 0.815299 | 9.927278e-52 | -8.615850e-47 |
| Msa0348370 | Msa0383770 | 0.820555 | 6.449335e-53 | -8.615850e-47 |
| Msa0354550 | Msa0383770 | 0.833581 | 4.912931e-56 | -8.615850e-47 |
| Msa0357470 | Msa0383770 | 0.802881 | 4.545705e-49 | -8.615850e-47 |
| Msa0357510 | Msa0383770 | 0.810692 | 1.015645e-50 | -8.615850e-47 |
| Msa0357530 | Msa0383770 | 0.806351 | 8.581317e-50 | -8.615850e-47 |
| Msa0364030 | Msa0383770 | -0.800504 | 1.397547e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0383770 | MtrunA17_Chr3g0140411 | 94.624 | 186 | 8 | 1 | 1 | 186 | 9 | 192 | 4.33e-131 | 366 |
| Msa0383770 | MtrunA17_Chr3g0140421 | 82.723 | 191 | 29 | 3 | 1 | 191 | 1 | 187 | 8.81e-114 | 322 |
| Msa0383770 | MtrunA17_Chr8g0340431 | 26.702 | 191 | 103 | 6 | 21 | 180 | 1 | 185 | 2.46e-13 | 65.5 |
| Msa0383770 | MtrunA17_Chr8g0340461 | 26.804 | 194 | 105 | 7 | 21 | 182 | 1 | 189 | 8.99e-13 | 63.9 |
| Msa0383770 | MtrunA17_Chr8g0340451 | 26.289 | 194 | 106 | 7 | 21 | 182 | 1 | 189 | 1.00e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0383770 | AT4G32860.1 | 33.971 | 209 | 99 | 8 | 1 | 185 | 1 | 194 | 7.24e-23 | 91.3 |
Find 43 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACGGCACACCTTCATTTGC+CGG | 0.291928 | 3_1:-86776042 | None:intergenic |
| GTAATATCTGTAACATTGTT+TGG | 0.307750 | 3_1:-86775683 | None:intergenic |
| TAATATCTGTAACATTGTTT+GGG | 0.386224 | 3_1:-86775682 | None:intergenic |
| GAGAGCTTAGGTTGCAGAAA+TGG | 0.410450 | 3_1:+86776179 | Msa0383770:CDS |
| TTGCCGGCGACCTTCCTCCT+TGG | 0.425099 | 3_1:-86776026 | None:intergenic |
| AGCAAAACGGTGCGTTTACT+AGG | 0.432952 | 3_1:+86775746 | Msa0383770:CDS |
| TCCTTTCATTGCTGCTTCCA+CGG | 0.447597 | 3_1:-86776060 | None:intergenic |
| GTCGGCACGTGAGTGCTTCA+TGG | 0.449621 | 3_1:-86775883 | None:intergenic |
| TATGTATTTCATTTCACCGT+TGG | 0.457166 | 3_1:+86775919 | Msa0383770:CDS |
| CGGCGACCTTCCTCCTTGGC+CGG | 0.461416 | 3_1:-86776022 | None:intergenic |
| CTATGAACTCCTCCGCCGCC+TGG | 0.473317 | 3_1:-86776144 | None:intergenic |
| TCATGTTGACCAGGCGGCGG+AGG | 0.488533 | 3_1:+86776135 | Msa0383770:CDS |
| AAGCTTCCTCCTACTACTGC+TGG | 0.509632 | 3_1:-86775993 | None:intergenic |
| GGAAGGTCGCCGGCAAATGA+AGG | 0.527011 | 3_1:+86776033 | Msa0383770:CDS |
| TTGTCTCCGGCCAAGGAGGA+AGG | 0.528048 | 3_1:+86776016 | Msa0383770:CDS |
| GCTTAGGTTGCAGAAATGGT+TGG | 0.533031 | 3_1:+86776183 | Msa0383770:CDS |
| CTAGGTTTCATGTTACAAAA+CGG | 0.539359 | 3_1:+86775764 | Msa0383770:CDS |
| ATGCAAGAAATGATGAAGAG+AGG | 0.551359 | 3_1:+86775818 | Msa0383770:CDS |
| GCGGTGGCGCCAGCAGTAGT+AGG | 0.554764 | 3_1:+86775984 | Msa0383770:CDS |
| GTTTCATGTTGACCAGGCGG+CGG | 0.558520 | 3_1:+86776132 | Msa0383770:CDS |
| GAAAGAGTTTCATGTTGACC+AGG | 0.578595 | 3_1:+86776126 | Msa0383770:CDS |
| CGGCCAAGGAGGAAGGTCGC+CGG | 0.581708 | 3_1:+86776023 | Msa0383770:CDS |
| TGTTACAGATATTACATGCA+TGG | 0.585321 | 3_1:+86775691 | Msa0383770:CDS |
| TGAAGATGACAACTTTGTCA+AGG | 0.599361 | 3_1:+86776089 | Msa0383770:CDS |
| TTTGTCAAGGGAGACTGAGA+AGG | 0.604036 | 3_1:+86776102 | Msa0383770:CDS |
| GTGGCGCCAGCAGTAGTAGG+AGG | 0.604984 | 3_1:+86775987 | Msa0383770:CDS |
| TTTCAATTACCACCGTTGGT+TGG | 0.610216 | 3_1:-86775718 | None:intergenic |
| GCCGTGGAAGCAGCAATGAA+AGG | 0.610528 | 3_1:+86776059 | Msa0383770:CDS |
| GCGCCACCGCGAGAGAGACG+CGG | 0.622064 | 3_1:-86775971 | None:intergenic |
| GAAGATGACAACTTTGTCAA+GGG | 0.623449 | 3_1:+86776090 | Msa0383770:CDS |
| ACATATCAGCATCACGTGGT+CGG | 0.624919 | 3_1:-86775901 | None:intergenic |
| CGGCAAATGAAGGTGTGCCG+TGG | 0.631422 | 3_1:+86776043 | Msa0383770:CDS |
| AGTCCACCGCGTCTCTCTCG+CGG | 0.632836 | 3_1:+86775965 | Msa0383770:CDS |
| GTGGAAGCAGCAATGAAAGG+AGG | 0.655270 | 3_1:+86776062 | Msa0383770:CDS |
| CAGCTGAACTGATACTCCAA+CGG | 0.655898 | 3_1:-86775935 | None:intergenic |
| CCACCGCGTCTCTCTCGCGG+TGG | 0.660075 | 3_1:+86775968 | Msa0383770:CDS |
| ATGCATGGATAACCAACCAA+CGG | 0.673857 | 3_1:+86775706 | Msa0383770:CDS |
| AGAGTTTCATGTTGACCAGG+CGG | 0.681822 | 3_1:+86776129 | Msa0383770:CDS |
| GAAAACACTGAACAACGTGA+TGG | 0.683073 | 3_1:+86775853 | Msa0383770:CDS |
| CATGGATAACCAACCAACGG+TGG | 0.704369 | 3_1:+86775709 | Msa0383770:CDS |
| CCACCGCGAGAGAGACGCGG+TGG | 0.712456 | 3_1:-86775968 | None:intergenic |
| AAATACATATCAGCATCACG+TGG | 0.752599 | 3_1:-86775905 | None:intergenic |
| AACTTCATACAGAAACAGAG+TGG | 0.756819 | 3_1:+86775654 | Msa0383770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATATCTGTAACATTGTTT+GGG | - | chr3_1:86775685-86775704 | None:intergenic | 20.0% |
| ! | GTAATATCTGTAACATTGTT+TGG | - | chr3_1:86775686-86775705 | None:intergenic | 25.0% |
| ! | AATTGAAAAAGTGAGCAAAA+CGG | + | chr3_1:86775733-86775752 | Msa0383770:CDS | 25.0% |
| TGTTACAGATATTACATGCA+TGG | + | chr3_1:86775691-86775710 | Msa0383770:CDS | 30.0% | |
| CTAGGTTTCATGTTACAAAA+CGG | + | chr3_1:86775764-86775783 | Msa0383770:CDS | 30.0% | |
| ATGAAGAGAGGAAAAAACAT+AGG | + | chr3_1:86775830-86775849 | Msa0383770:CDS | 30.0% | |
| TGAAGAGAGGAAAAAACATA+GGG | + | chr3_1:86775831-86775850 | Msa0383770:CDS | 30.0% | |
| TATGTATTTCATTTCACCGT+TGG | + | chr3_1:86775919-86775938 | Msa0383770:CDS | 30.0% | |
| AACTTCATACAGAAACAGAG+TGG | + | chr3_1:86775654-86775673 | Msa0383770:CDS | 35.0% | |
| !!! | ACTTTTTCAATTACCACCGT+TGG | - | chr3_1:86775725-86775744 | None:intergenic | 35.0% |
| ATGCAAGAAATGATGAAGAG+AGG | + | chr3_1:86775818-86775837 | Msa0383770:CDS | 35.0% | |
| AAATACATATCAGCATCACG+TGG | - | chr3_1:86775908-86775927 | None:intergenic | 35.0% | |
| TGAAGATGACAACTTTGTCA+AGG | + | chr3_1:86776089-86776108 | Msa0383770:CDS | 35.0% | |
| GAAGATGACAACTTTGTCAA+GGG | + | chr3_1:86776090-86776109 | Msa0383770:CDS | 35.0% | |
| ! | ACAAGTTTTACAGAGAGCTT+AGG | + | chr3_1:86776167-86776186 | Msa0383770:CDS | 35.0% |
| ATGCATGGATAACCAACCAA+CGG | + | chr3_1:86775706-86775725 | Msa0383770:CDS | 40.0% | |
| TTTCAATTACCACCGTTGGT+TGG | - | chr3_1:86775721-86775740 | None:intergenic | 40.0% | |
| GAAAACACTGAACAACGTGA+TGG | + | chr3_1:86775853-86775872 | Msa0383770:CDS | 40.0% | |
| GAAAGAGTTTCATGTTGACC+AGG | + | chr3_1:86776126-86776145 | Msa0383770:CDS | 40.0% | |
| AGCAAAACGGTGCGTTTACT+AGG | + | chr3_1:86775746-86775765 | Msa0383770:CDS | 45.0% | |
| ACATATCAGCATCACGTGGT+CGG | - | chr3_1:86775904-86775923 | None:intergenic | 45.0% | |
| CAGCTGAACTGATACTCCAA+CGG | - | chr3_1:86775938-86775957 | None:intergenic | 45.0% | |
| ! | TAGGAGGAAGCTTTTGTCTC+CGG | + | chr3_1:86776003-86776022 | Msa0383770:CDS | 45.0% |
| ! | TCCTTTCATTGCTGCTTCCA+CGG | - | chr3_1:86776063-86776082 | None:intergenic | 45.0% |
| TTTGTCAAGGGAGACTGAGA+AGG | + | chr3_1:86776102-86776121 | Msa0383770:CDS | 45.0% | |
| AGAGTTTCATGTTGACCAGG+CGG | + | chr3_1:86776129-86776148 | Msa0383770:CDS | 45.0% | |
| ! | GAGAGCTTAGGTTGCAGAAA+TGG | + | chr3_1:86776179-86776198 | Msa0383770:CDS | 45.0% |
| ! | GCTTAGGTTGCAGAAATGGT+TGG | + | chr3_1:86776183-86776202 | Msa0383770:CDS | 45.0% |
| CATGGATAACCAACCAACGG+TGG | + | chr3_1:86775709-86775728 | Msa0383770:CDS | 50.0% | |
| AAGCTTCCTCCTACTACTGC+TGG | - | chr3_1:86775996-86776015 | None:intergenic | 50.0% | |
| GTGGAAGCAGCAATGAAAGG+AGG | + | chr3_1:86776062-86776081 | Msa0383770:CDS | 50.0% | |
| ! | GAAGCTTTTGTCTCCGGCCA+AGG | + | chr3_1:86776009-86776028 | Msa0383770:CDS | 55.0% |
| CACGGCACACCTTCATTTGC+CGG | - | chr3_1:86776045-86776064 | None:intergenic | 55.0% | |
| GCCGTGGAAGCAGCAATGAA+AGG | + | chr3_1:86776059-86776078 | Msa0383770:CDS | 55.0% | |
| GTTTCATGTTGACCAGGCGG+CGG | + | chr3_1:86776132-86776151 | Msa0383770:CDS | 55.0% | |
| GTCGGCACGTGAGTGCTTCA+TGG | - | chr3_1:86775886-86775905 | None:intergenic | 60.0% | |
| ! | GCTTTTGTCTCCGGCCAAGG+AGG | + | chr3_1:86776012-86776031 | Msa0383770:CDS | 60.0% |
| TTGTCTCCGGCCAAGGAGGA+AGG | + | chr3_1:86776016-86776035 | Msa0383770:CDS | 60.0% | |
| GGAAGGTCGCCGGCAAATGA+AGG | + | chr3_1:86776033-86776052 | Msa0383770:CDS | 60.0% | |
| ! | CGGCAAATGAAGGTGTGCCG+TGG | + | chr3_1:86776043-86776062 | Msa0383770:CDS | 60.0% |
| AGTCCACCGCGTCTCTCTCG+CGG | + | chr3_1:86775965-86775984 | Msa0383770:CDS | 65.0% | |
| GTGGCGCCAGCAGTAGTAGG+AGG | + | chr3_1:86775987-86776006 | Msa0383770:CDS | 65.0% | |
| TTGCCGGCGACCTTCCTCCT+TGG | - | chr3_1:86776029-86776048 | None:intergenic | 65.0% | |
| TCATGTTGACCAGGCGGCGG+AGG | + | chr3_1:86776135-86776154 | Msa0383770:CDS | 65.0% | |
| CTATGAACTCCTCCGCCGCC+TGG | - | chr3_1:86776147-86776166 | None:intergenic | 65.0% | |
| GCGGTGGCGCCAGCAGTAGT+AGG | + | chr3_1:86775984-86776003 | Msa0383770:CDS | 70.0% | |
| CGGCGACCTTCCTCCTTGGC+CGG | - | chr3_1:86776025-86776044 | None:intergenic | 70.0% | |
| CGGCCAAGGAGGAAGGTCGC+CGG | + | chr3_1:86776023-86776042 | Msa0383770:CDS | 70.0% | |
| CCACCGCGAGAGAGACGCGG+TGG | - | chr3_1:86775971-86775990 | None:intergenic | 75.0% | |
| CCACCGCGTCTCTCTCGCGG+TGG | + | chr3_1:86775968-86775987 | Msa0383770:CDS | 75.0% | |
| GCGCCACCGCGAGAGAGACG+CGG | - | chr3_1:86775974-86775993 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 86775650 | 86776231 | 86775650 | ID=Msa0383770;Name=Msa0383770 |
| chr3_1 | mRNA | 86775650 | 86776231 | 86775650 | ID=Msa0383770-mRNA-1;Parent=Msa0383770;Name=Msa0383770-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|193 |
| chr3_1 | exon | 86775650 | 86776231 | 86775650 | ID=Msa0383770-mRNA-1:exon:22982;Parent=Msa0383770-mRNA-1 |
| chr3_1 | CDS | 86775650 | 86776231 | 86775650 | ID=Msa0383770-mRNA-1:cds;Parent=Msa0383770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
