AlfalfaGEDB | Msa0488820

M. sativa cultivar zhongmu-4 / Msa0488820

KEGG: K00652
GO: -
PFAM: PF00155.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488820	XP_003598166.2	95.789	475	18	1	1	475	1	473	0.0	902

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488820	sp\|Q8GW43\|BIOF_ARATH	69.762	463	126	2	12	464	6	464	0.0	662

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488820	G7IVJ8	95.789	475	18	1	1	475	1	473	0.0	902

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002750	Msa0488820	0.807244	5.555363e-50	-8.615850e-47
Msa0002790	Msa0488820	0.805801	1.120118e-49	-8.615850e-47
Msa0116670	Msa0488820	0.805732	1.158286e-49	-8.615850e-47
Msa0173530	Msa0488820	0.807397	5.157109e-50	-8.615850e-47
Msa0348990	Msa0488820	0.967659	1.392828e-127	-8.615850e-47
Msa0393550	Msa0488820	0.863302	2.742243e-64	-8.615850e-47
Msa0462110	Msa0488820	0.962089	1.829292e-120	-8.615850e-47
Msa0488820	Msa1215820	0.802511	5.420313e-49	-8.615850e-47
Msa0488820	Msa1338190	0.804851	1.771124e-49	-8.615850e-47
Msa0488820	Msa1343260	0.804118	2.518228e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488820	MtrunA17_Chr3g0078851	95.789	475	18	1	1	475	1	473	0.0	902
Msa0488820	MtrunA17_Chr6g0461991	26.176	340	205	6	144	457	143	462	8.70e-26	110
Msa0488820	MtrunA17_Chr3g0099931	23.117	385	243	13	102	455	110	472	9.99e-19	88.6
Msa0488820	MtrunA17_Chr5g0430851	22.857	385	244	13	102	455	110	472	4.09e-18	86.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488820	AT5G04620.2	69.762	463	126	2	12	464	6	464	0.0	662
Msa0488820	AT5G04620.3	70.000	410	109	2	12	411	6	411	0.0	581
Msa0488820	AT5G04620.1	76.133	331	79	0	134	464	1	331	0.0	527
Msa0488820	AT5G23670.1	25.982	331	199	7	144	448	143	453	4.53e-26	111
Msa0488820	AT5G23670.2	25.982	331	199	7	144	448	143	453	4.53e-26	111
Msa0488820	AT3G48780.2	25.982	331	199	7	144	448	143	453	2.80e-25	108
Msa0488820	AT3G48780.1	25.982	331	199	7	144	448	143	453	2.80e-25	108
Msa0488820	AT3G48790.1	25.994	327	203	9	141	448	4	310	1.07e-22	99.4
Msa0488820	AT3G48790.2	27.170	265	168	8	203	448	20	278	1.74e-19	89.7
Msa0488820	AT4G36480.2	21.898	274	184	6	102	365	109	362	7.02e-16	80.5

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Find 137 sgRNAs with CRISPR-Local

Find 422 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGAGAATAAGGTTT+TGG	0.290579	3_4:-8946337	None:intergenic
AAACTCACAAGAGTGTATTG+AGG	0.681743	3_4:-8949081	None:intergenic
AAAGACTTTCATTTGCTTAC+AGG	0.206039	3_4:+8951317	Msa0488820:CDS
AAAGATAATTTACCGGAACT+TGG	0.420440	3_4:-8946618	None:intergenic
AAAGCTGCCGGCTGCCATGG+AGG	0.624806	3_4:+8950459	Msa0488820:CDS
AAATGGTGGTGGTGTTGCTG+AGG	0.647397	3_4:+8950389	Msa0488820:CDS
AAATTCGTGAAACCACGTTT+AGG	0.399614	3_4:+8946577	Msa0488820:CDS
AACAGGCATTTATTACAAGC+TGG	0.479117	3_4:+8951541	Msa0488820:intron
AAGAACATGGGATGGGACCA+AGG	0.538361	3_4:+8947897	Msa0488820:CDS
AAGATAATTTACCGGAACTT+GGG	0.449828	3_4:-8946617	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAGTAACTTCTTTCAACAA+TGG	+	chr3_4:8946371-8946390	Msa0488820:exon	30.0%
AAGATAATTTACCGGAACTT+GGG	-	chr3_4:8946620-8946639	None:intergenic	30.0%
AAAGATAATTTACCGGAACT+TGG	-	chr3_4:8946621-8946640	None:intergenic	30.0%
CATAAATCATAACAGAACAC+AGG	-	chr3_4:8947343-8947362	None:intergenic	30.0%
TGCAAATAACAAGAATAGAG+CGG	-	chr3_4:8947494-8947513	None:intergenic	30.0%
TTGTGTTCTGTTATGATTTC+GGG	+	chr3_4:8947557-8947576	Msa0488820:intron	30.0%
TTAGGTGAAGAGATTGTTTA+TGG	+	chr3_4:8947623-8947642	Msa0488820:intron	30.0%
GTGGATATACCAATTATCAT+AGG	+	chr3_4:8947936-8947955	Msa0488820:CDS	30.0%
AACAAAACCAGAACAGTTTA+AGG	-	chr3_4:8948024-8948043	None:intergenic	30.0%
AATGTTCAAACTAGGACATA+AGG	-	chr3_4:8948178-8948197	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	8946315	8952709	8946315	ID=Msa0488820;Name=Msa0488820
chr3_4	mRNA	8946315	8952709	8946315	ID=Msa0488820-mRNA-1;Parent=Msa0488820;Name=Msa0488820-mRNA-1;_AED=0.16;_eAED=0.16;_QI=75\|1\|1\|1\|0.9\|1\|11\|204\|475
chr3_4	exon	8946315	8946627	8946315	ID=Msa0488820-mRNA-1:exon:2872;Parent=Msa0488820-mRNA-1
chr3_4	exon	8947627	8947769	8947627	ID=Msa0488820-mRNA-1:exon:2873;Parent=Msa0488820-mRNA-1
chr3_4	exon	8947890	8947997	8947890	ID=Msa0488820-mRNA-1:exon:2874;Parent=Msa0488820-mRNA-1
chr3_4	exon	8948857	8949095	8948857	ID=Msa0488820-mRNA-1:exon:2875;Parent=Msa0488820-mRNA-1
chr3_4	exon	8949192	8949233	8949192	ID=Msa0488820-mRNA-1:exon:2876;Parent=Msa0488820-mRNA-1
chr3_4	exon	8949953	8950040	8949953	ID=Msa0488820-mRNA-1:exon:2877;Parent=Msa0488820-mRNA-1
chr3_4	exon	8950363	8950496	8950363	ID=Msa0488820-mRNA-1:exon:2878;Parent=Msa0488820-mRNA-1
chr3_4	exon	8950649	8950737	8950649	ID=Msa0488820-mRNA-1:exon:2879;Parent=Msa0488820-mRNA-1
chr3_4	exon	8951258	8951408	8951258	ID=Msa0488820-mRNA-1:exon:2880;Parent=Msa0488820-mRNA-1
chr3_4	exon	8951546	8951614	8951546	ID=Msa0488820-mRNA-1:exon:2881;Parent=Msa0488820-mRNA-1
chr3_4	exon	8952379	8952709	8952379	ID=Msa0488820-mRNA-1:exon:2882;Parent=Msa0488820-mRNA-1
chr3_4	five_prime_UTR	8946315	8946389	8946315	ID=Msa0488820-mRNA-1:five_prime_utr;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8946390	8946627	8946390	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8947627	8947769	8947627	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8947890	8947997	8947890	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8948857	8949095	8948857	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8949192	8949233	8949192	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8949953	8950040	8949953	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8950363	8950496	8950363	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8950649	8950737	8950649	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8951258	8951408	8951258	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8951546	8951614	8951546	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	CDS	8952379	8952505	8952379	ID=Msa0488820-mRNA-1:cds;Parent=Msa0488820-mRNA-1
chr3_4	three_prime_UTR	8952506	8952709	8952506	ID=Msa0488820-mRNA-1:three_prime_utr;Parent=Msa0488820-mRNA-1

Gene Sequence

>Msa0488820

ATGGAGGCACCAATACCAATACCAAGTGCATGGTGGCAAAAATGGGTCGAAGAGGCACTAACAACCCTTGATTCTCTCAGAGTACTTCGATCTCTACGACCCATTTGTCTCCAACGCGGGGTAAATGATGATGCGTTTGAGGTGTTTGATGAAATGCAGCAATGGGATCGTTCTTCTGTTGAGGTTCAAATTCGTGAAACCACGTTTAGGAAATGGATGCATGATACCCCAAGTTCCGGTGAAGAGATTGTTTATGGTGCAGTTTCTGGTGATGATGAAGCAGGGTTATGTCATGAGAAGTTTAGAAAATTAATTTTGTTTTCTGGAAATGATTATTTAGGCCTGAGTTCCCATCCGACTATTGGAAAGGCTGCTGCAAAGGCAGCTCAAGAACATGGGATGGGACCAAGGGGTTCTGCTCTTATTTGTGGATATACCAATTATCATAGGCAGCTAGAGTCGAGTTTGGCAGATTTGAAAAAGAAAGAGGATTGCCTTCTTTGTCCTACAGGATTTGCTGCCAATATGGCCTTGATGACAGCAATTGGAAGTATTGGTTCTCTCTTAGCTGGGAATAGCATACCTTCAGATGATGAAAAGATTGCTATCTTTTCTGATGCATTAAATCACGCGTCAATAATTGATGGTATTCGTCTTGCTGAGCGGCAAAAAGGGGTTAAACCGTACATATATAGACATTGTGACATGTCCCACCTCAATACACTCTTATCTCATTGCAGTATGAAGAGAAAAATTGTGATAACCGACAGCTTGTTTAGCATGGATGGGGACTTTGCACCAATGGTTGAGCTTGCAGAACTCCGCAAGAAGCATGGCTTTTTGTTAGTCATCGATGATGCTCATGGGACATTTGTTTGTGGCAAAAATGGTGGTGGTGTTGCTGAGGAGTTCAATTGTGAGAAGGATGTGGATATTTGCATTGGTACACTAAGTAAAGCTGCCGGCTGCCATGGAGGATTTATAGCTTGCAGCACATCGTGGAAGTTATTAATACAGTCAAGAGGTCGTTCATTTATATTTTCAACTGCTACGCCGGTTCCAGTTGCTGCTGCTGCTCATGCTGCTGTTAGAGTGGCAAAACATGAGACATGGCGTAGAAAGGCCATTTGGAACCGGGTGAAAGACTTTCATTTGCTTACAGGAATCCCTGTTACAAGTCCCATCATATCCCTTATCGTTGGCACTGAAGACAAAGCACTGCAAGCAAGCCGGCATTTATTACAAGCTGGCTTCCATGTGACTGCAATCAGACCACCAACTGTGCCTCCTAACTCCTGCAGGTTGCGAGTGGCCCTCAGTGCAGTCCATACAAGGGAAGATTTGGAAAACTTAGCAGCCGCACTCTCGAATTGCATCAATTTCCAAGATACACGAATTTACGGCTGCAATGGCTATGCAAGATTATAG

Protein sequence

>Msa0488820

MEAPIPIPSAWWQKWVEEALTTLDSLRVLRSLRPICLQRGVNDDAFEVFDEMQQWDRSSVEVQIRETTFRKWMHDTPSSGEEIVYGAVSGDDEAGLCHEKFRKLILFSGNDYLGLSSHPTIGKAAAKAAQEHGMGPRGSALICGYTNYHRQLESSLADLKKKEDCLLCPTGFAANMALMTAIGSIGSLLAGNSIPSDDEKIAIFSDALNHASIIDGIRLAERQKGVKPYIYRHCDMSHLNTLLSHCSMKRKIVITDSLFSMDGDFAPMVELAELRKKHGFLLVIDDAHGTFVCGKNGGGVAEEFNCEKDVDICIGTLSKAAGCHGGFIACSTSWKLLIQSRGRSFIFSTATPVPVAAAAHAAVRVAKHETWRRKAIWNRVKDFHLLTGIPVTSPIISLIVGTEDKALQASRHLLQAGFHVTAIRPPTVPPNSCRLRVALSAVHTREDLENLAAALSNCINFQDTRIYGCNGYARL*