AlfalfaGEDB | Msa1215820

M. sativa cultivar zhongmu-4 / Msa1215820

KEGG: K02949
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
PFAM: PF16205.8

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1215820	XP_003609505.1	98.750	160	2	0	1	160	1	160	4.49e-112	325

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1215820	sp\|P17093\|RS11_SOYBN	95.000	160	7	1	1	160	1	159	1.37e-98	283

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1215820	B7FMB4	98.750	160	2	0	1	160	1	160	2.15e-112	325

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002750	Msa1215820	0.996333	3.325023e-226	-8.615850e-47
Msa0002790	Msa1215820	0.982656	1.171200e-155	-8.615850e-47
Msa0003780	Msa1215820	0.869277	3.491736e-66	-8.615850e-47
Msa0022970	Msa1215820	0.868552	5.998773e-66	-8.615850e-47
Msa0027260	Msa1215820	0.848977	4.344998e-60	-8.615850e-47
Msa0032780	Msa1215820	0.818710	1.700925e-52	-8.615850e-47
Msa0040690	Msa1215820	0.827348	1.644495e-54	-8.615850e-47
Msa0042280	Msa1215820	0.830336	3.109739e-55	-8.615850e-47
Msa0043660	Msa1215820	0.801010	1.101653e-48	-8.615850e-47
Msa0044890	Msa1215820	0.838342	3.043542e-57	-8.615850e-47
Msa0045730	Msa1215820	0.847981	8.195274e-60	-8.615850e-47
Msa0047160	Msa1215820	0.824172	9.323809e-54	-8.615850e-47
Msa0048180	Msa1215820	0.855832	4.840999e-62	-8.615850e-47
Msa0049370	Msa1215820	0.823786	1.148331e-53	-8.615850e-47
Msa0055180	Msa1215820	0.819599	1.067333e-52	-8.615850e-47
Msa0056470	Msa1215820	0.821385	4.153549e-53	-8.615850e-47
Msa0068970	Msa1215820	0.831213	1.895795e-55	-8.615850e-47
Msa0074030	Msa1215820	0.865405	6.047078e-65	-8.615850e-47
Msa0077530	Msa1215820	0.822069	2.885686e-53	-8.615850e-47
Msa0086750	Msa1215820	0.904675	9.576640e-80	-8.615850e-47
Msa0091880	Msa1215820	0.829979	3.801845e-55	-8.615850e-47
Msa0094470	Msa1215820	0.824424	8.131054e-54	-8.615850e-47
Msa0097580	Msa1215820	0.836940	6.968407e-57	-8.615850e-47
Msa0097600	Msa1215820	0.812114	4.989691e-51	-8.615850e-47
Msa0100740	Msa1215820	0.811602	6.449811e-51	-8.615850e-47
Msa0116130	Msa1215820	0.847568	1.064945e-59	-8.615850e-47
Msa0116670	Msa1215820	0.877481	6.071320e-69	-8.615850e-47
Msa0118710	Msa1215820	0.874662	5.667052e-68	-8.615850e-47
Msa0603320	Msa1215820	0.859905	2.992709e-63	-8.615850e-47
Msa0615440	Msa1215820	0.832909	7.220886e-56	-8.615850e-47
Msa0615580	Msa1215820	0.812475	4.162001e-51	-8.615850e-47
Msa0620890	Msa1215820	0.816052	6.745118e-52	-8.615850e-47
Msa0621950	Msa1215820	0.835180	1.950141e-56	-8.615850e-47
Msa0622750	Msa1215820	0.860274	2.316149e-63	-8.615850e-47
Msa0623200	Msa1215820	0.848639	5.390617e-60	-8.615850e-47
Msa0642160	Msa1215820	0.813466	2.523722e-51	-8.615850e-47
Msa0644350	Msa1215820	0.830947	2.203578e-55	-8.615850e-47
Msa0661300	Msa1215820	0.824590	7.434643e-54	-8.615850e-47
Msa0664560	Msa1215820	0.801076	1.067901e-48	-8.615850e-47
Msa0666230	Msa1215820	0.844886	5.725398e-59	-8.615850e-47
Msa0688850	Msa1215820	0.826170	3.141627e-54	-8.615850e-47
Msa0698490	Msa1215820	0.834033	3.785846e-56	-8.615850e-47
Msa0698660	Msa1215820	0.832720	8.047714e-56	-8.615850e-47
Msa0698780	Msa1215820	0.851740	7.291087e-61	-8.615850e-47
Msa0712610	Msa1215820	0.861733	8.335126e-64	-8.615850e-47
Msa0719420	Msa1215820	0.809723	1.643495e-50	-8.615850e-47
Msa0729200	Msa1215820	0.864917	8.608057e-65	-8.615850e-47
Msa0738100	Msa1215820	0.859520	3.908447e-63	-8.615850e-47
Msa0752910	Msa1215820	0.801696	7.972904e-49	-8.615850e-47
Msa0753140	Msa1215820	0.807073	6.039139e-50	-8.615850e-47
Msa0769590	Msa1215820	0.868661	5.531690e-66	-8.615850e-47
Msa0780490	Msa1215820	0.835356	1.759494e-56	-8.615850e-47
Msa0781080	Msa1215820	0.811099	8.291492e-51	-8.615850e-47
Msa0785130	Msa1215820	0.858368	8.643220e-63	-8.615850e-47
Msa0789680	Msa1215820	0.810147	1.332110e-50	-8.615850e-47
Msa0792420	Msa1215820	0.837561	4.835195e-57	-8.615850e-47
Msa0792760	Msa1215820	0.823238	1.542441e-53	-8.615850e-47
Msa0806880	Msa1215820	0.879230	1.476024e-69	-8.615850e-47
Msa0817540	Msa1215820	0.884900	1.291582e-71	-8.615850e-47
Msa0835420	Msa1215820	0.801569	8.464039e-49	-8.615850e-47
Msa0128860	Msa1215820	0.864803	9.348623e-65	-8.615850e-47
Msa0133540	Msa1215820	0.898133	7.150687e-77	-8.615850e-47
Msa0140850	Msa1215820	0.800560	1.361049e-48	-8.615850e-47
Msa0146340	Msa1215820	0.891144	5.192254e-74	-8.615850e-47
Msa0163760	Msa1215820	0.836315	1.005702e-56	-8.615850e-47
Msa0167480	Msa1215820	0.825213	5.299268e-54	-8.615850e-47
Msa0172900	Msa1215820	0.819754	9.838434e-53	-8.615850e-47
Msa0173530	Msa1215820	0.879104	1.635420e-69	-8.615850e-47
Msa0174730	Msa1215820	0.863972	1.699145e-64	-8.615850e-47
Msa0181490	Msa1215820	0.888516	5.507764e-73	-8.615850e-47
Msa0183090	Msa1215820	0.855284	6.996779e-62	-8.615850e-47
Msa0187930	Msa1215820	0.858596	7.389505e-63	-8.615850e-47
Msa0189700	Msa1215820	0.879420	1.264659e-69	-8.615850e-47
Msa0192230	Msa1215820	0.805091	1.577960e-49	-8.615850e-47
Msa0194540	Msa1215820	0.848429	6.163184e-60	-8.615850e-47
Msa0210330	Msa1215820	0.818485	1.912585e-52	-8.615850e-47
Msa0210360	Msa1215820	0.859942	2.917262e-63	-8.615850e-47
Msa0218830	Msa1215820	0.860454	2.043033e-63	-8.615850e-47
Msa0219120	Msa1215820	0.846396	2.229636e-59	-8.615850e-47
Msa0221030	Msa1215820	0.845020	5.269547e-59	-8.615850e-47
Msa0222730	Msa1215820	0.822368	2.458715e-53	-8.615850e-47
Msa0230490	Msa1215820	0.855146	7.670508e-62	-8.615850e-47
Msa0231100	Msa1215820	0.838803	2.314576e-57	-8.615850e-47
Msa0231660	Msa1215820	0.801811	7.549586e-49	-8.615850e-47
Msa0232010	Msa1215820	0.878118	3.637936e-69	-8.615850e-47
Msa0232080	Msa1215820	0.878875	1.970411e-69	-8.615850e-47
Msa0366890	Msa1215820	0.807005	6.243771e-50	-8.615850e-47
Msa0369450	Msa1215820	0.810056	1.393466e-50	-8.615850e-47
Msa0393370	Msa1215820	0.823245	1.536708e-53	-8.615850e-47
Msa0395890	Msa1215820	0.807624	4.616268e-50	-8.615850e-47
Msa0414800	Msa1215820	0.804402	2.198279e-49	-8.615850e-47
Msa0443390	Msa1215820	0.849869	2.451791e-60	-8.615850e-47
Msa0462110	Msa1215820	0.817940	2.540604e-52	-8.615850e-47
Msa0481850	Msa1215820	0.879301	1.393579e-69	-8.615850e-47
Msa0989540	Msa1215820	0.816033	6.812809e-52	-8.615850e-47
Msa1006840	Msa1215820	0.861397	1.055875e-63	-8.615850e-47
Msa1010310	Msa1215820	0.842351	2.727044e-58	-8.615850e-47
Msa1012740	Msa1215820	0.844490	7.320038e-59	-8.615850e-47
Msa1013400	Msa1215820	0.832076	1.162047e-55	-8.615850e-47
Msa1019240	Msa1215820	0.857944	1.155408e-62	-8.615850e-47
Msa1019260	Msa1215820	0.864248	1.394065e-64	-8.615850e-47
Msa1039520	Msa1215820	0.817283	3.574098e-52	-8.615850e-47
Msa1043690	Msa1215820	0.854195	1.446405e-61	-8.615850e-47
Msa1050210	Msa1215820	0.800362	1.493600e-48	-8.615850e-47
Msa1051880	Msa1215820	0.803500	3.383676e-49	-8.615850e-47
Msa1072710	Msa1215820	0.854098	1.542673e-61	-8.615850e-47
Msa1078870	Msa1215820	0.864798	9.380245e-65	-8.615850e-47
Msa1078980	Msa1215820	0.844630	6.712525e-59	-8.615850e-47
Msa1080350	Msa1215820	0.906311	1.698868e-80	-8.615850e-47
Msa1081150	Msa1215820	0.813398	2.611582e-51	-8.615850e-47
Msa1088490	Msa1215820	0.908860	1.076371e-81	-8.615850e-47
Msa1088940	Msa1215820	0.808738	2.672485e-50	-8.615850e-47
Msa1093530	Msa1215820	0.828074	1.100463e-54	-8.615850e-47
Msa1094380	Msa1215820	0.809920	1.490706e-50	-8.615850e-47
Msa1104140	Msa1215820	0.867798	1.049513e-65	-8.615850e-47
Msa1104210	Msa1215820	0.859830	3.153289e-63	-8.615850e-47
Msa0243140	Msa1215820	0.807946	3.941946e-50	-8.615850e-47
Msa0244780	Msa1215820	0.865405	6.048741e-65	-8.615850e-47
Msa0250820	Msa1215820	0.895400	9.934593e-76	-8.615850e-47
Msa0250870	Msa1215820	0.852098	5.768905e-61	-8.615850e-47
Msa0257450	Msa1215820	0.880181	6.780072e-70	-8.615850e-47
Msa0258850	Msa1215820	0.870559	1.331744e-66	-8.615850e-47
Msa0262480	Msa1215820	0.823491	1.346026e-53	-8.615850e-47
Msa0264010	Msa1215820	0.878926	1.891405e-69	-8.615850e-47
Msa0268130	Msa1215820	0.852509	4.406203e-61	-8.615850e-47
Msa0269180	Msa1215820	0.811654	6.285200e-51	-8.615850e-47
Msa0289580	Msa1215820	0.906384	1.571462e-80	-8.615850e-47
Msa0289610	Msa1215820	0.908702	1.280197e-81	-8.615850e-47
Msa0296720	Msa1215820	0.849173	3.831529e-60	-8.615850e-47
Msa0297830	Msa1215820	0.809747	1.623720e-50	-8.615850e-47
Msa0298200	Msa1215820	0.864171	1.473132e-64	-8.615850e-47
Msa0298990	Msa1215820	0.860492	1.989046e-63	-8.615850e-47
Msa0300350	Msa1215820	0.826923	2.077898e-54	-8.615850e-47
Msa0306690	Msa1215820	0.881970	1.541498e-70	-8.615850e-47
Msa0312210	Msa1215820	0.802250	6.132037e-49	-8.615850e-47
Msa0313100	Msa1215820	0.833626	4.786854e-56	-8.615850e-47
Msa0317910	Msa1215820	0.820031	8.503029e-53	-8.615850e-47
Msa0321640	Msa1215820	0.847201	1.342998e-59	-8.615850e-47
Msa0327300	Msa1215820	0.910503	1.742544e-82	-8.615850e-47
Msa0327330	Msa1215820	0.912719	1.411493e-83	-8.615850e-47
Msa0335840	Msa1215820	0.817491	3.208019e-52	-8.615850e-47
Msa0336010	Msa1215820	0.885947	5.237744e-72	-8.615850e-47
Msa0337670	Msa1215820	0.843386	1.446560e-58	-8.615850e-47
Msa0341880	Msa1215820	0.820603	6.287582e-53	-8.615850e-47
Msa0342850	Msa1215820	0.846697	1.845293e-59	-8.615850e-47
Msa0348830	Msa1215820	0.859859	3.089193e-63	-8.615850e-47
Msa0348990	Msa1215820	0.814096	1.833603e-51	-8.615850e-47
Msa1124850	Msa1215820	0.824296	8.716928e-54	-8.615850e-47
Msa1125860	Msa1215820	0.911188	8.068101e-83	-8.615850e-47
Msa1126060	Msa1215820	0.906202	1.908094e-80	-8.615850e-47
Msa1126510	Msa1215820	0.845497	3.915553e-59	-8.615850e-47
Msa1126830	Msa1215820	0.800225	1.592868e-48	-8.615850e-47
Msa1128120	Msa1215820	0.831894	1.288862e-55	-8.615850e-47
Msa1135890	Msa1215820	0.826295	2.933558e-54	-8.615850e-47
Msa1163530	Msa1215820	0.821549	3.805992e-53	-8.615850e-47
Msa1166860	Msa1215820	0.858371	8.627210e-63	-8.615850e-47
Msa1166870	Msa1215820	0.835949	1.245768e-56	-8.615850e-47
Msa1167540	Msa1215820	0.841147	5.668215e-58	-8.615850e-47
Msa1167550	Msa1215820	0.837584	4.769127e-57	-8.615850e-47
Msa1169180	Msa1215820	0.840766	7.135748e-58	-8.615850e-47
Msa1181830	Msa1215820	0.825711	4.038868e-54	-8.615850e-47
Msa1181840	Msa1215820	0.812961	3.257647e-51	-8.615850e-47
Msa1181850	Msa1215820	0.832986	6.912691e-56	-8.615850e-47
Msa1181860	Msa1215820	0.813752	2.183406e-51	-8.615850e-47
Msa1207270	Msa1215820	0.826958	2.038563e-54	-8.615850e-47
Msa1213480	Msa1215820	0.817339	3.470790e-52	-8.615850e-47
Msa1215820	Msa1218600	0.810294	1.238201e-50	-8.615850e-47
Msa1215820	Msa1230580	0.816523	5.294077e-52	-8.615850e-47
Msa1215820	Msa1256840	0.812946	3.282588e-51	-8.615850e-47
Msa1215820	Msa1257510	0.873319	1.612419e-67	-8.615850e-47
Msa1215820	Msa1260530	0.811257	7.662421e-51	-8.615850e-47
Msa1215820	Msa1265630	0.825607	4.275080e-54	-8.615850e-47
Msa1215820	Msa1266060	0.801133	1.039472e-48	-8.615850e-47
Msa1215820	Msa1273340	0.828482	8.771013e-55	-8.615850e-47
Msa1215820	Msa1295150	0.815941	7.140552e-52	-8.615850e-47
Msa1215820	Msa1295170	0.833748	4.463609e-56	-8.615850e-47
Msa1215820	Msa1299590	0.829795	4.213878e-55	-8.615850e-47
Msa1215820	Msa1300380	0.813727	2.210917e-51	-8.615850e-47
Msa1215820	Msa1305050	0.843891	1.059942e-58	-8.615850e-47
Msa1215820	Msa1311400	0.820175	7.880508e-53	-8.615850e-47
Msa1215820	Msa1321790	0.839483	1.542358e-57	-8.615850e-47
Msa1215820	Msa1331710	0.822621	2.147493e-53	-8.615850e-47
Msa1215820	Msa1332250	0.837952	3.835679e-57	-8.615850e-47
Msa1215820	Msa1338190	0.877880	4.404975e-69	-8.615850e-47
Msa1215820	Msa1343260	0.882457	1.025213e-70	-8.615850e-47
Msa1215820	Msa1349570	0.855680	5.363449e-62	-8.615850e-47
Msa1215820	Msa1356790	0.874587	6.012306e-68	-8.615850e-47
Msa1215820	Msa1362790	0.841707	4.035231e-58	-8.615850e-47
Msa1215820	Msa1363330	0.890790	7.165644e-74	-8.615850e-47
Msa1215820	Msa1366180	0.912481	1.854859e-83	-8.615850e-47
Msa1215820	Msa1369190	0.837392	5.339535e-57	-8.615850e-47
Msa1215820	Msa1374120	0.857246	1.860257e-62	-8.615850e-47
Msa1215820	Msa1379760	0.865076	7.675459e-65	-8.615850e-47
Msa1215820	Msa1382620	0.824660	7.156625e-54	-8.615850e-47
Msa1215820	Msa1386600	0.891404	4.096581e-74	-8.615850e-47
Msa1215820	Msa1387410	0.853555	2.211658e-61	-8.615850e-47
Msa1215820	Msa1394290	0.816339	5.818930e-52	-8.615850e-47
Msa1215820	Msa1399530	0.860993	1.401930e-63	-8.615850e-47
Msa1215820	Msa1407070	0.838521	2.737520e-57	-8.615850e-47
Msa1215820	Msa1413500	0.879458	1.226254e-69	-8.615850e-47
Msa1215820	Msa1418690	0.815020	1.144724e-51	-8.615850e-47
Msa1215820	Msa1418760	0.829764	4.288925e-55	-8.615850e-47
Msa1215820	Msa1426120	0.871112	8.754229e-67	-8.615850e-47
Msa1215820	Msa1426370	0.848988	4.315346e-60	-8.615850e-47
Msa1215820	Msa1426490	0.919330	5.150481e-87	-8.615850e-47
Msa1215820	Msa1427560	0.851774	7.131030e-61	-8.615850e-47
Msa1215820	Msa1432700	0.855517	5.984609e-62	-8.615850e-47
Msa1215820	Msa1433310	0.848697	5.197030e-60	-8.615850e-47
Msa1215820	Msa1448690	0.863358	2.635310e-64	-8.615850e-47
Msa1215820	Msa1450280	0.844894	5.698085e-59	-8.615850e-47
Msa1215820	Msa1456050	0.874609	5.909513e-68	-8.615850e-47
Msa1215820	Msa1457920	0.810331	1.215564e-50	-8.615850e-47
Msa1215820	Msa1465260	0.820299	7.381211e-53	-8.615850e-47
Msa0483110	Msa1215820	0.869898	2.192663e-66	-8.615850e-47
Msa0486070	Msa1215820	0.841513	4.540021e-58	-8.615850e-47
Msa0488820	Msa1215820	0.802511	5.420313e-49	-8.615850e-47
Msa0521050	Msa1215820	0.852751	3.758179e-61	-8.615850e-47
Msa0526540	Msa1215820	0.887641	1.193709e-72	-8.615850e-47
Msa0527150	Msa1215820	0.832201	1.082100e-55	-8.615850e-47
Msa0531650	Msa1215820	0.823809	1.133986e-53	-8.615850e-47
Msa0571920	Msa1215820	0.841057	5.983282e-58	-8.615850e-47
Msa0575170	Msa1215820	0.810023	1.416282e-50	-8.615850e-47
Msa0576940	Msa1215820	0.882219	1.251459e-70	-8.615850e-47
Msa0581110	Msa1215820	0.800382	1.479564e-48	-8.615850e-47
Msa0584030	Msa1215820	0.805928	1.053328e-49	-8.615850e-47
Msa0588360	Msa1215820	0.866429	2.869617e-65	-8.615850e-47
Msa0867060	Msa1215820	0.804369	2.232416e-49	-8.615850e-47
Msa0876170	Msa1215820	0.809150	2.181632e-50	-8.615850e-47
Msa0881290	Msa1215820	0.879123	1.610468e-69	-8.615850e-47
Msa0888810	Msa1215820	0.822686	2.074597e-53	-8.615850e-47
Msa0902760	Msa1215820	0.831520	1.593741e-55	-8.615850e-47
Msa0930490	Msa1215820	0.818167	2.257838e-52	-8.615850e-47
Msa0945480	Msa1215820	0.815376	9.543642e-52	-8.615850e-47
Msa0953310	Msa1215820	0.896250	4.417469e-76	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1215820	MtrunA17_Chr4g0065131	98.750	160	2	0	1	160	1	160	4.13e-116	325
Msa1215820	MtrunA17_Chr8g0372611	98.750	160	2	0	1	160	1	160	1.17e-115	323
Msa1215820	MtrunA17_Chr1g0152301	93.789	161	9	1	1	160	1	161	6.37e-100	284

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1215820	AT3G48930.1	91.250	160	14	0	1	160	1	160	3.55e-97	277
Msa1215820	AT5G23740.1	90.000	160	15	1	1	160	1	159	2.67e-95	273
Msa1215820	AT4G30800.1	90.625	160	14	1	1	160	1	159	1.41e-93	268

Find 71 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTAAAATGGCGAAATAAATT+AGG	0.187394	8_1:+77241528	Msa1215820:five_prime_UTR
AATACAAGCGCTTTCTTATC+AGG	0.209242	8_1:-77243818	None:intergenic
GAAGGAGATCATGTTATTAT+TGG	0.232109	8_1:+77243311	Msa1215820:CDS
ATGGAAACATTGCCAGTAAA+AGG	0.250015	8_1:-77243043	None:intergenic
GGAACCTTCAATGGCTTCTC+TGG	0.256456	8_1:-77241985	None:intergenic
ATTGCTCCGGTCCAGTAAAA+TGG	0.334230	8_1:+77241514	Msa1215820:five_prime_UTR
CCATAGCATTGTAATAAAAT+CGG	0.339068	8_1:+77241484	Msa1215820:five_prime_UTR
TTCTTTAAATCCGTTGGTCT+TGG	0.342978	8_1:+77241951	Msa1215820:CDS
AGATGTGATGAGAGTAATTC+TGG	0.347310	8_1:+77243569	Msa1215820:exon
AAGGAGATCATGTTATTATT+GGG	0.351302	8_1:+77243312	Msa1215820:CDS
GCTGGTTCATCCAGTGGTGC+TGG	0.354296	8_1:+77243522	Msa1215820:CDS
TAAAATGGCGAAATAAATTA+GGG	0.356524	8_1:+77241529	Msa1215820:five_prime_UTR
ACCCTCTCACTGAAATTTGT+TGG	0.362133	8_1:-77241563	None:intergenic
TGAATAGGACTATTATTGTT+AGG	0.366373	8_1:+77243107	Msa1215820:CDS
GCTGGGAAGAAGGCATTTAC+TGG	0.369417	8_1:+77243540	Msa1215820:CDS
AGCAACCTAAAGTGTTTCTA+TGG	0.384927	8_1:+77241779	Msa1215820:CDS
ATCACCCTGCTTCCGTGTTA+AGG	0.389482	8_1:+77243289	Msa1215820:CDS
ACATACCATAGAAACACTTT+AGG	0.392416	8_1:-77241784	None:intergenic
TCCTTCCTTAACACGGAAGC+AGG	0.400654	8_1:-77243294	None:intergenic
TTACTGGCAATGTTTCCATC+CGG	0.434194	8_1:+77243047	Msa1215820:CDS
TACTGGCAATGTTTCCATCC+GGG	0.437436	8_1:+77243048	Msa1215820:CDS
GTCACAGTGCTAAGATGAAT+AGG	0.443080	8_1:+77243092	Msa1215820:CDS
AACCGCTTCTTTAAATCCGT+TGG	0.460564	8_1:+77241945	Msa1215820:CDS
AAAGCAAAACAAAATTGAAC+AGG	0.460920	8_1:-77243618	None:intergenic
AGGGCACTTCTTGTCAATGT+AGG	0.469506	8_1:-77243023	None:intergenic
GACGTTGAACCTCACAGTCT+TGG	0.472416	8_1:-77243484	None:intergenic
TGCCTTCTTCCCAGCACCAC+TGG	0.477160	8_1:-77243532	None:intergenic
TCCAACAAATTTCAGTGAGA+GGG	0.477757	8_1:+77241562	Msa1215820:five_prime_UTR
ATCCAGTGGTGCTGGGAAGA+AGG	0.479938	8_1:+77243530	Msa1215820:CDS
CAGCCATGGTGAAGAAGCTT+CGG	0.482612	8_1:-77241631	None:intergenic
ATGTTATTATTGGGCAATGC+AGG	0.485129	8_1:+77243321	Msa1215820:CDS
TTGAAGTTAAATTCTGTCGT+TGG	0.486172	8_1:+77243640	Msa1215820:three_prime_UTR
CTGGTTCATCCAGTGGTGCT+GGG	0.492604	8_1:+77243523	Msa1215820:CDS
AATCGGGTTCATTATTGCTC+CGG	0.493717	8_1:+77241501	Msa1215820:five_prime_UTR
CATAGCATTGTAATAAAATC+GGG	0.494684	8_1:+77241485	Msa1215820:five_prime_UTR
GTTAATTACTTGTTCAGCCA+TGG	0.496408	8_1:-77241645	None:intergenic
AAAGGAAAGAGGCCTGGAAA+AGG	0.502986	8_1:+77241918	Msa1215820:CDS
AGCTAAAATACGGCCCCGGA+TGG	0.510722	8_1:-77243062	None:intergenic
GACAAGTTCCAGCTAAAATA+CGG	0.511848	8_1:-77243072	None:intergenic
TGTCGTTGGATACAAGTTGA+AGG	0.514126	8_1:+77243654	Msa1215820:three_prime_UTR
TTCCAACAAATTTCAGTGAG+AGG	0.514757	8_1:+77241561	Msa1215820:five_prime_UTR
TGGAAACATTGCCAGTAAAA+GGG	0.518269	8_1:-77243042	None:intergenic
ACTCCCAGAGAAGCCATTGA+AGG	0.523893	8_1:+77241981	Msa1215820:CDS
TTGGTAGCTCAAAGAAGAGT+GGG	0.529884	8_1:+77241895	Msa1215820:intron
GCACCACTGGATGAACCAGC+TGG	0.536114	8_1:-77243519	None:intergenic
AGTGGGAAAGGAAAGAGGCC+TGG	0.536739	8_1:+77241912	Msa1215820:CDS
GAGTAATTCTGGTTTCTGTA+TGG	0.556390	8_1:+77243580	Msa1215820:three_prime_UTR
CCCTGCTTCCGTGTTAAGGA+AGG	0.558291	8_1:+77243293	Msa1215820:CDS
GAACCTTCAATGGCTTCTCT+GGG	0.562688	8_1:-77241984	None:intergenic
GTCTTGAAAGTGATCCCAGC+TGG	0.565686	8_1:+77243504	Msa1215820:CDS
ATCCCAGCTGGTTCATCCAG+TGG	0.566568	8_1:+77243516	Msa1215820:CDS
AGAAGAGTGGGAAAGGAAAG+AGG	0.567345	8_1:+77241907	Msa1215820:CDS
ACTGGCAATGTTTCCATCCG+GGG	0.570865	8_1:+77243049	Msa1215820:CDS
AAGCAGGGTGATACATGAGC+AGG	0.574164	8_1:-77243278	None:intergenic
GGAAAGAGGCCTGGAAAAGG+TGG	0.575072	8_1:+77241921	Msa1215820:CDS
TTGTGTGTTACAGACTGAGA+AGG	0.577565	8_1:+77241747	Msa1215820:intron
TTCCAGCTAAAATACGGCCC+CGG	0.583185	8_1:-77243066	None:intergenic
ATAGGACTATTATTGTTAGG+AGG	0.587464	8_1:+77243110	Msa1215820:CDS
AGCTCAAAGAAGAGTGGGAA+AGG	0.587623	8_1:+77241900	Msa1215820:intron
GACCAACGGATTTAAAGAAG+CGG	0.587920	8_1:-77241947	None:intergenic
TTTGGTAGCTCAAAGAAGAG+TGG	0.594148	8_1:+77241894	Msa1215820:intron
CTCTATTTAGATATGAGAAG+AGG	0.599289	8_1:+77243243	Msa1215820:intron
AACCTCACAGTCTTGGAGAG+AGG	0.610094	8_1:-77243477	None:intergenic
ATTCCGAAGCTTCTTCACCA+TGG	0.616570	8_1:+77241628	Msa1215820:exon
CATGATCTCCTTCCTTAACA+CGG	0.626861	8_1:-77243301	None:intergenic
CACCACTGGATGAACCAGCT+GGG	0.630037	8_1:-77243518	None:intergenic
CCTTCCTTAACACGGAAGCA+GGG	0.633141	8_1:-77243293	None:intergenic
ACTTTATCAAGAAGTATCAG+AGG	0.634593	8_1:+77243143	Msa1215820:CDS
GGCCTCTCTCCAAGACTGTG+AGG	0.650917	8_1:+77243475	Msa1215820:intron
AGTCTTAAATCCAAGACCAA+CGG	0.666807	8_1:-77241961	None:intergenic
GAAGCTTCGGAATTTCGCAG+AGG	0.714721	8_1:-77241618	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTTTATTTTAAAAAT+AGG	+	chr8_1:77242998-77243017	Msa1215820:intron	0.0%
!!!	ATTTTTTTTATTTTAAAAAT+AGG	+	chr8_1:77242998-77243017	Msa1215820:intron	0.0%
!!!	AATTTATTGTGATATTTTAT+TGG	+	chr8_1:77242028-77242047	Msa1215820:intron	10.0%
!!!	AAAAAAACTTAATTGAATTT+TGG	+	chr8_1:77243738-77243757	Msa1215820:intron	10.0%
!!!	AATTTATTGTGATATTTTAT+TGG	+	chr8_1:77242028-77242047	Msa1215820:intron	10.0%
!!!	AAAAAAACTTAATTGAATTT+TGG	+	chr8_1:77243738-77243757	Msa1215820:intron	10.0%
!!!	AAAAAAGGATAAATCATTTT+GGG	-	chr8_1:77241823-77241842	None:intergenic	15.0%
!!!	AAAAAAAGGATAAATCATTT+TGG	-	chr8_1:77241824-77241843	None:intergenic	15.0%
!!!	AAAAAAGGATAAATCATTTT+GGG	-	chr8_1:77241823-77241842	None:intergenic	15.0%
!!!	AAAAAAAGGATAAATCATTT+TGG	-	chr8_1:77241824-77241843	None:intergenic	15.0%
!!	TAAAATGGCGAAATAAATTA+GGG	+	chr8_1:77241529-77241548	Msa1215820:five_prime_UTR	20.0%
!!!	GTTTGTTATTTGATTGAATT+TGG	+	chr8_1:77241876-77241895	Msa1215820:intron	20.0%
!!!	TGTGCAATAAATGTTTTAAT+TGG	+	chr8_1:77242220-77242239	Msa1215820:intron	20.0%
!!	ACATCATATATTAAAGACTA+TGG	-	chr8_1:77242401-77242420	None:intergenic	20.0%
!!!	AATACAAAATGGATTTCAAA+AGG	-	chr8_1:77242698-77242717	None:intergenic	20.0%
!!!	ATAATTTAAGACACAGAAAA+AGG	-	chr8_1:77243366-77243385	None:intergenic	20.0%
!!!	AAGGATTTAGATTTTCATTA+TGG	+	chr8_1:77243673-77243692	Msa1215820:three_prime_UTR	20.0%
!!	TAAAATGGCGAAATAAATTA+GGG	+	chr8_1:77241529-77241548	Msa1215820:five_prime_UTR	20.0%
!!!	GTTTGTTATTTGATTGAATT+TGG	+	chr8_1:77241876-77241895	Msa1215820:intron	20.0%
!!!	TGTGCAATAAATGTTTTAAT+TGG	+	chr8_1:77242220-77242239	Msa1215820:intron	20.0%
!!	ACATCATATATTAAAGACTA+TGG	-	chr8_1:77242401-77242420	None:intergenic	20.0%
!!!	AATACAAAATGGATTTCAAA+AGG	-	chr8_1:77242698-77242717	None:intergenic	20.0%
!!!	ATAATTTAAGACACAGAAAA+AGG	-	chr8_1:77243366-77243385	None:intergenic	20.0%
!!!	AAGGATTTAGATTTTCATTA+TGG	+	chr8_1:77243673-77243692	Msa1215820:three_prime_UTR	20.0%
!	CCATAGCATTGTAATAAAAT+CGG	+	chr8_1:77241484-77241503	Msa1215820:five_prime_UTR	25.0%
!	CATAGCATTGTAATAAAATC+GGG	+	chr8_1:77241485-77241504	Msa1215820:five_prime_UTR	25.0%
!!!	AATTTATTTCGCCATTTTAC+TGG	-	chr8_1:77241528-77241547	None:intergenic	25.0%
!	GTAAAATGGCGAAATAAATT+AGG	+	chr8_1:77241528-77241547	Msa1215820:five_prime_UTR	25.0%
!!	TTGTGATATTTTATTGGATC+TGG	+	chr8_1:77242034-77242053	Msa1215820:intron	25.0%
!!	TCACAAATAGTCAATTTTGT+AGG	+	chr8_1:77242087-77242106	Msa1215820:intron	25.0%
!!!	TGATGTTTTAATGTATGATG+TGG	+	chr8_1:77242415-77242434	Msa1215820:intron	25.0%
!	ACATCAATGCATTTACATTT+CGG	-	chr8_1:77242589-77242608	None:intergenic	25.0%
!	AAAGAAACAACAATGTCATT+TGG	-	chr8_1:77242872-77242891	None:intergenic	25.0%
!	AAAGAAACAACAAAGTCATT+TGG	-	chr8_1:77242932-77242951	None:intergenic	25.0%
!	TGAATAGGACTATTATTGTT+AGG	+	chr8_1:77243107-77243126	Msa1215820:CDS	25.0%
!	AAGGAGATCATGTTATTATT+GGG	+	chr8_1:77243312-77243331	Msa1215820:CDS	25.0%
!	AAAGCAAAACAAAATTGAAC+AGG	-	chr8_1:77243621-77243640	None:intergenic	25.0%
!!!	TTTTTGATTTTTTAGACGTG+AGG	-	chr8_1:77243785-77243804	None:intergenic	25.0%
!	CCATAGCATTGTAATAAAAT+CGG	+	chr8_1:77241484-77241503	Msa1215820:five_prime_UTR	25.0%
!	CATAGCATTGTAATAAAATC+GGG	+	chr8_1:77241485-77241504	Msa1215820:five_prime_UTR	25.0%
!!!	AATTTATTTCGCCATTTTAC+TGG	-	chr8_1:77241528-77241547	None:intergenic	25.0%
!	GTAAAATGGCGAAATAAATT+AGG	+	chr8_1:77241528-77241547	Msa1215820:five_prime_UTR	25.0%
!!	TTGTGATATTTTATTGGATC+TGG	+	chr8_1:77242034-77242053	Msa1215820:intron	25.0%
!!	TCACAAATAGTCAATTTTGT+AGG	+	chr8_1:77242087-77242106	Msa1215820:intron	25.0%
!!!	TGATGTTTTAATGTATGATG+TGG	+	chr8_1:77242415-77242434	Msa1215820:intron	25.0%
!	ACATCAATGCATTTACATTT+CGG	-	chr8_1:77242589-77242608	None:intergenic	25.0%
!	AAAGAAACAACAATGTCATT+TGG	-	chr8_1:77242872-77242891	None:intergenic	25.0%
!	AAAGAAACAACAAAGTCATT+TGG	-	chr8_1:77242932-77242951	None:intergenic	25.0%
!	TGAATAGGACTATTATTGTT+AGG	+	chr8_1:77243107-77243126	Msa1215820:CDS	25.0%
!	AAGGAGATCATGTTATTATT+GGG	+	chr8_1:77243312-77243331	Msa1215820:CDS	25.0%
!	AAAGCAAAACAAAATTGAAC+AGG	-	chr8_1:77243621-77243640	None:intergenic	25.0%
!!!	TTTTTGATTTTTTAGACGTG+AGG	-	chr8_1:77243785-77243804	None:intergenic	25.0%
!	CCGATTTTATTACAATGCTA+TGG	-	chr8_1:77241487-77241506	None:intergenic	30.0%
!!	TTCCTTTTTCCCTTTTGTTT+CGG	+	chr8_1:77241674-77241693	Msa1215820:intron	30.0%
	TAAGAACAACCGAAACAAAA+GGG	-	chr8_1:77241686-77241705	None:intergenic	30.0%
	ACATACCATAGAAACACTTT+AGG	-	chr8_1:77241787-77241806	None:intergenic	30.0%
	CAACAAATCGAAGGAAAAAA+AGG	-	chr8_1:77241838-77241857	None:intergenic	30.0%
	TCAAAATCACAACAAATCGA+AGG	-	chr8_1:77241847-77241866	None:intergenic	30.0%
	ACAATAAATTCACAATGCAG+AGG	-	chr8_1:77242018-77242037	None:intergenic	30.0%
	GAATTTCAACAAAATCACAG+TGG	-	chr8_1:77242273-77242292	None:intergenic	30.0%
!!	AAAGTATAGCCTTTGAGTTT+TGG	+	chr8_1:77242293-77242312	Msa1215820:intron	30.0%
	GAAGATTAATCTTTGATAGG+TGG	-	chr8_1:77242351-77242370	None:intergenic	30.0%
	CAGGAAGATTAATCTTTGAT+AGG	-	chr8_1:77242354-77242373	None:intergenic	30.0%
	CATGTGGCTGAATTTATTAT+TGG	+	chr8_1:77242440-77242459	Msa1215820:intron	30.0%
	TTCTGTCATGCAATCATTAT+TGG	+	chr8_1:77242608-77242627	Msa1215820:intron	30.0%
!!	AGCATATTATTTTGCTAGCT+AGG	-	chr8_1:77242662-77242681	None:intergenic	30.0%
	CATTGGACTCTAATACAAAA+TGG	-	chr8_1:77242709-77242728	None:intergenic	30.0%
!	CATTTTGTATTAGAGTCCAA+TGG	+	chr8_1:77242707-77242726	Msa1215820:intron	30.0%
!	ACAACAATGTCATTTGGATT+TGG	-	chr8_1:77242866-77242885	None:intergenic	30.0%
	CAGAAATAAACATGCACATT+CGG	-	chr8_1:77242895-77242914	None:intergenic	30.0%
	ATAGGACTATTATTGTTAGG+AGG	+	chr8_1:77243110-77243129	Msa1215820:CDS	30.0%
!!	ACTTTATCAAGAAGTATCAG+AGG	+	chr8_1:77243143-77243162	Msa1215820:CDS	30.0%
	CAAATAAAAACAGGACATCA+AGG	-	chr8_1:77243175-77243194	None:intergenic	30.0%
	CTCTATTTAGATATGAGAAG+AGG	+	chr8_1:77243243-77243262	Msa1215820:intron	30.0%
	GAAGGAGATCATGTTATTAT+TGG	+	chr8_1:77243311-77243330	Msa1215820:CDS	30.0%
	GATATAGATGTATGTCTGAT+TGG	+	chr8_1:77243430-77243449	Msa1215820:intron	30.0%
!!	TTTATTTGTGCCATTTCATC+AGG	+	chr8_1:77243454-77243473	Msa1215820:intron	30.0%
	TTGAAGTTAAATTCTGTCGT+TGG	+	chr8_1:77243640-77243659	Msa1215820:three_prime_UTR	30.0%
!	CCGATTTTATTACAATGCTA+TGG	-	chr8_1:77241487-77241506	None:intergenic	30.0%
!!	TTCCTTTTTCCCTTTTGTTT+CGG	+	chr8_1:77241674-77241693	Msa1215820:intron	30.0%
	TAAGAACAACCGAAACAAAA+GGG	-	chr8_1:77241686-77241705	None:intergenic	30.0%
	ACATACCATAGAAACACTTT+AGG	-	chr8_1:77241787-77241806	None:intergenic	30.0%
	CAACAAATCGAAGGAAAAAA+AGG	-	chr8_1:77241838-77241857	None:intergenic	30.0%
	TCAAAATCACAACAAATCGA+AGG	-	chr8_1:77241847-77241866	None:intergenic	30.0%
	ACAATAAATTCACAATGCAG+AGG	-	chr8_1:77242018-77242037	None:intergenic	30.0%
	GAATTTCAACAAAATCACAG+TGG	-	chr8_1:77242273-77242292	None:intergenic	30.0%
!!	AAAGTATAGCCTTTGAGTTT+TGG	+	chr8_1:77242293-77242312	Msa1215820:intron	30.0%
	GAAGATTAATCTTTGATAGG+TGG	-	chr8_1:77242351-77242370	None:intergenic	30.0%
	CAGGAAGATTAATCTTTGAT+AGG	-	chr8_1:77242354-77242373	None:intergenic	30.0%
	CATGTGGCTGAATTTATTAT+TGG	+	chr8_1:77242440-77242459	Msa1215820:intron	30.0%
	TTCTGTCATGCAATCATTAT+TGG	+	chr8_1:77242608-77242627	Msa1215820:intron	30.0%
!!	AGCATATTATTTTGCTAGCT+AGG	-	chr8_1:77242662-77242681	None:intergenic	30.0%
	CATTGGACTCTAATACAAAA+TGG	-	chr8_1:77242709-77242728	None:intergenic	30.0%
!	CATTTTGTATTAGAGTCCAA+TGG	+	chr8_1:77242707-77242726	Msa1215820:intron	30.0%
!	ACAACAATGTCATTTGGATT+TGG	-	chr8_1:77242866-77242885	None:intergenic	30.0%
	CAGAAATAAACATGCACATT+CGG	-	chr8_1:77242895-77242914	None:intergenic	30.0%
	ATAGGACTATTATTGTTAGG+AGG	+	chr8_1:77243110-77243129	Msa1215820:CDS	30.0%
!!	ACTTTATCAAGAAGTATCAG+AGG	+	chr8_1:77243143-77243162	Msa1215820:CDS	30.0%
	CAAATAAAAACAGGACATCA+AGG	-	chr8_1:77243175-77243194	None:intergenic	30.0%
	CTCTATTTAGATATGAGAAG+AGG	+	chr8_1:77243243-77243262	Msa1215820:intron	30.0%
	GAAGGAGATCATGTTATTAT+TGG	+	chr8_1:77243311-77243330	Msa1215820:CDS	30.0%
	GATATAGATGTATGTCTGAT+TGG	+	chr8_1:77243430-77243449	Msa1215820:intron	30.0%
!!	TTTATTTGTGCCATTTCATC+AGG	+	chr8_1:77243454-77243473	Msa1215820:intron	30.0%
	TTGAAGTTAAATTCTGTCGT+TGG	+	chr8_1:77243640-77243659	Msa1215820:three_prime_UTR	30.0%
	TTCCAACAAATTTCAGTGAG+AGG	+	chr8_1:77241561-77241580	Msa1215820:five_prime_UTR	35.0%
	TCCAACAAATTTCAGTGAGA+GGG	+	chr8_1:77241562-77241581	Msa1215820:five_prime_UTR	35.0%
	GTTAATTACTTGTTCAGCCA+TGG	-	chr8_1:77241648-77241667	None:intergenic	35.0%
	AACCGAAACAAAAGGGAAAA+AGG	-	chr8_1:77241679-77241698	None:intergenic	35.0%
	CTAAGAACAACCGAAACAAA+AGG	-	chr8_1:77241687-77241706	None:intergenic	35.0%
!	AGCAACCTAAAGTGTTTCTA+TGG	+	chr8_1:77241779-77241798	Msa1215820:CDS	35.0%
!	TTCTTTAAATCCGTTGGTCT+TGG	+	chr8_1:77241951-77241970	Msa1215820:CDS	35.0%
	AGTCTTAAATCCAAGACCAA+CGG	-	chr8_1:77241964-77241983	None:intergenic	35.0%
	GGTAAAAAAGGAACCTTCAA+TGG	-	chr8_1:77241997-77242016	None:intergenic	35.0%
	TCACAATGCAGAGGTAAAAA+AGG	-	chr8_1:77242009-77242028	None:intergenic	35.0%
	AATGTATGATGTGGATCATG+TGG	+	chr8_1:77242424-77242443	Msa1215820:intron	35.0%
!!!	AAGCTTTTGATTTCGAAACC+AGG	+	chr8_1:77242483-77242502	Msa1215820:intron	35.0%
!!!	AGCTTTTGATTTCGAAACCA+GGG	+	chr8_1:77242484-77242503	Msa1215820:intron	35.0%
	AAACACATGATACAAATCCC+TGG	-	chr8_1:77242504-77242523	None:intergenic	35.0%
!!	CCTATACTTTCAAGTTGGAT+TGG	-	chr8_1:77242555-77242574	None:intergenic	35.0%
!	CCAATCCAACTTGAAAGTAT+AGG	+	chr8_1:77242552-77242571	Msa1215820:intron	35.0%
!	GTAGACCTATACTTTCAAGT+TGG	-	chr8_1:77242560-77242579	None:intergenic	35.0%
	GACAACAAGTGTTATGACTA+GGG	-	chr8_1:77242743-77242762	None:intergenic	35.0%
	TGACAACAAGTGTTATGACT+AGG	-	chr8_1:77242744-77242763	None:intergenic	35.0%
	TGGAAACATTGCCAGTAAAA+GGG	-	chr8_1:77243045-77243064	None:intergenic	35.0%
	ATGGAAACATTGCCAGTAAA+AGG	-	chr8_1:77243046-77243065	None:intergenic	35.0%
	GACAAGTTCCAGCTAAAATA+CGG	-	chr8_1:77243075-77243094	None:intergenic	35.0%
!!!	GTCCTGTTTTTATTTGCACT+TGG	+	chr8_1:77243179-77243198	Msa1215820:intron	35.0%
	CTCCAAGTGCAAATAAAAAC+AGG	-	chr8_1:77243184-77243203	None:intergenic	35.0%
	ATGTTATTATTGGGCAATGC+AGG	+	chr8_1:77243321-77243340	Msa1215820:CDS	35.0%
	AGATGTGATGAGAGTAATTC+TGG	+	chr8_1:77243569-77243588	Msa1215820:exon	35.0%
!!	GAGTAATTCTGGTTTCTGTA+TGG	+	chr8_1:77243580-77243599	Msa1215820:three_prime_UTR	35.0%
!!!	GATTTTTTAGACGTGAGGTA+AGG	-	chr8_1:77243780-77243799	None:intergenic	35.0%
	AATACAAGCGCTTTCTTATC+AGG	-	chr8_1:77243821-77243840	None:intergenic	35.0%
	TTCCAACAAATTTCAGTGAG+AGG	+	chr8_1:77241561-77241580	Msa1215820:five_prime_UTR	35.0%
	TCCAACAAATTTCAGTGAGA+GGG	+	chr8_1:77241562-77241581	Msa1215820:five_prime_UTR	35.0%
	GTTAATTACTTGTTCAGCCA+TGG	-	chr8_1:77241648-77241667	None:intergenic	35.0%
	AACCGAAACAAAAGGGAAAA+AGG	-	chr8_1:77241679-77241698	None:intergenic	35.0%
	CTAAGAACAACCGAAACAAA+AGG	-	chr8_1:77241687-77241706	None:intergenic	35.0%
!	AGCAACCTAAAGTGTTTCTA+TGG	+	chr8_1:77241779-77241798	Msa1215820:CDS	35.0%
!	TTCTTTAAATCCGTTGGTCT+TGG	+	chr8_1:77241951-77241970	Msa1215820:CDS	35.0%
	AGTCTTAAATCCAAGACCAA+CGG	-	chr8_1:77241964-77241983	None:intergenic	35.0%
	GGTAAAAAAGGAACCTTCAA+TGG	-	chr8_1:77241997-77242016	None:intergenic	35.0%
	TCACAATGCAGAGGTAAAAA+AGG	-	chr8_1:77242009-77242028	None:intergenic	35.0%
	AATGTATGATGTGGATCATG+TGG	+	chr8_1:77242424-77242443	Msa1215820:intron	35.0%
!!!	AAGCTTTTGATTTCGAAACC+AGG	+	chr8_1:77242483-77242502	Msa1215820:intron	35.0%
!!!	AGCTTTTGATTTCGAAACCA+GGG	+	chr8_1:77242484-77242503	Msa1215820:intron	35.0%
	AAACACATGATACAAATCCC+TGG	-	chr8_1:77242504-77242523	None:intergenic	35.0%
!!	CCTATACTTTCAAGTTGGAT+TGG	-	chr8_1:77242555-77242574	None:intergenic	35.0%
!	CCAATCCAACTTGAAAGTAT+AGG	+	chr8_1:77242552-77242571	Msa1215820:intron	35.0%
!	GTAGACCTATACTTTCAAGT+TGG	-	chr8_1:77242560-77242579	None:intergenic	35.0%
	GACAACAAGTGTTATGACTA+GGG	-	chr8_1:77242743-77242762	None:intergenic	35.0%
	TGACAACAAGTGTTATGACT+AGG	-	chr8_1:77242744-77242763	None:intergenic	35.0%
	TGGAAACATTGCCAGTAAAA+GGG	-	chr8_1:77243045-77243064	None:intergenic	35.0%
	ATGGAAACATTGCCAGTAAA+AGG	-	chr8_1:77243046-77243065	None:intergenic	35.0%
	GACAAGTTCCAGCTAAAATA+CGG	-	chr8_1:77243075-77243094	None:intergenic	35.0%
!!!	GTCCTGTTTTTATTTGCACT+TGG	+	chr8_1:77243179-77243198	Msa1215820:intron	35.0%
	CTCCAAGTGCAAATAAAAAC+AGG	-	chr8_1:77243184-77243203	None:intergenic	35.0%
	ATGTTATTATTGGGCAATGC+AGG	+	chr8_1:77243321-77243340	Msa1215820:CDS	35.0%
	AGATGTGATGAGAGTAATTC+TGG	+	chr8_1:77243569-77243588	Msa1215820:exon	35.0%
!!	GAGTAATTCTGGTTTCTGTA+TGG	+	chr8_1:77243580-77243599	Msa1215820:three_prime_UTR	35.0%
!!!	GATTTTTTAGACGTGAGGTA+AGG	-	chr8_1:77243780-77243799	None:intergenic	35.0%
	AATACAAGCGCTTTCTTATC+AGG	-	chr8_1:77243821-77243840	None:intergenic	35.0%
	AATCGGGTTCATTATTGCTC+CGG	+	chr8_1:77241501-77241520	Msa1215820:five_prime_UTR	40.0%
!!	ATTTCGCCATTTTACTGGAC+CGG	-	chr8_1:77241523-77241542	None:intergenic	40.0%
	ACCCTCTCACTGAAATTTGT+TGG	-	chr8_1:77241566-77241585	None:intergenic	40.0%
	TTGTGTGTTACAGACTGAGA+AGG	+	chr8_1:77241747-77241766	Msa1215820:intron	40.0%
!	TTTGGTAGCTCAAAGAAGAG+TGG	+	chr8_1:77241894-77241913	Msa1215820:intron	40.0%
!	TTGGTAGCTCAAAGAAGAGT+GGG	+	chr8_1:77241895-77241914	Msa1215820:intron	40.0%
	AACCGCTTCTTTAAATCCGT+TGG	+	chr8_1:77241945-77241964	Msa1215820:CDS	40.0%
!	GACCAACGGATTTAAAGAAG+CGG	-	chr8_1:77241950-77241969	None:intergenic	40.0%
!!	GCCTTTGAGTTTTGGCATAA+TGG	+	chr8_1:77242301-77242320	Msa1215820:intron	40.0%
	GCCATTATGCCAAAACTCAA+AGG	-	chr8_1:77242305-77242324	None:intergenic	40.0%
	CTAGGGATATCGAAAACCAT+TGG	-	chr8_1:77242726-77242745	None:intergenic	40.0%
	TGTTGTCATTGATCGTGTCA+TGG	+	chr8_1:77242756-77242775	Msa1215820:intron	40.0%
	TTACTGGCAATGTTTCCATC+CGG	+	chr8_1:77243047-77243066	Msa1215820:CDS	40.0%
	GTCACAGTGCTAAGATGAAT+AGG	+	chr8_1:77243092-77243111	Msa1215820:CDS	40.0%
	CATGATCTCCTTCCTTAACA+CGG	-	chr8_1:77243304-77243323	None:intergenic	40.0%
	TGTGTGTGTGTGTGTGTAAA+CGG	+	chr8_1:77243401-77243420	Msa1215820:intron	40.0%
!	TGTCGTTGGATACAAGTTGA+AGG	+	chr8_1:77243654-77243673	Msa1215820:three_prime_UTR	40.0%
	AATCGGGTTCATTATTGCTC+CGG	+	chr8_1:77241501-77241520	Msa1215820:five_prime_UTR	40.0%
!!	ATTTCGCCATTTTACTGGAC+CGG	-	chr8_1:77241523-77241542	None:intergenic	40.0%
	ACCCTCTCACTGAAATTTGT+TGG	-	chr8_1:77241566-77241585	None:intergenic	40.0%
	TTGTGTGTTACAGACTGAGA+AGG	+	chr8_1:77241747-77241766	Msa1215820:intron	40.0%
!	TTTGGTAGCTCAAAGAAGAG+TGG	+	chr8_1:77241894-77241913	Msa1215820:intron	40.0%
!	TTGGTAGCTCAAAGAAGAGT+GGG	+	chr8_1:77241895-77241914	Msa1215820:intron	40.0%
	AACCGCTTCTTTAAATCCGT+TGG	+	chr8_1:77241945-77241964	Msa1215820:CDS	40.0%
!	GACCAACGGATTTAAAGAAG+CGG	-	chr8_1:77241950-77241969	None:intergenic	40.0%
!!	GCCTTTGAGTTTTGGCATAA+TGG	+	chr8_1:77242301-77242320	Msa1215820:intron	40.0%
	GCCATTATGCCAAAACTCAA+AGG	-	chr8_1:77242305-77242324	None:intergenic	40.0%
	CTAGGGATATCGAAAACCAT+TGG	-	chr8_1:77242726-77242745	None:intergenic	40.0%
	TGTTGTCATTGATCGTGTCA+TGG	+	chr8_1:77242756-77242775	Msa1215820:intron	40.0%
	TTACTGGCAATGTTTCCATC+CGG	+	chr8_1:77243047-77243066	Msa1215820:CDS	40.0%
	GTCACAGTGCTAAGATGAAT+AGG	+	chr8_1:77243092-77243111	Msa1215820:CDS	40.0%
	CATGATCTCCTTCCTTAACA+CGG	-	chr8_1:77243304-77243323	None:intergenic	40.0%
	TGTGTGTGTGTGTGTGTAAA+CGG	+	chr8_1:77243401-77243420	Msa1215820:intron	40.0%
!	TGTCGTTGGATACAAGTTGA+AGG	+	chr8_1:77243654-77243673	Msa1215820:three_prime_UTR	40.0%
	ATTGCTCCGGTCCAGTAAAA+TGG	+	chr8_1:77241514-77241533	Msa1215820:five_prime_UTR	45.0%
	ATTCCGAAGCTTCTTCACCA+TGG	+	chr8_1:77241628-77241647	Msa1215820:exon	45.0%
	AGCTCAAAGAAGAGTGGGAA+AGG	+	chr8_1:77241900-77241919	Msa1215820:intron	45.0%
	AGAAGAGTGGGAAAGGAAAG+AGG	+	chr8_1:77241907-77241926	Msa1215820:CDS	45.0%
	AAAGGAAAGAGGCCTGGAAA+AGG	+	chr8_1:77241918-77241937	Msa1215820:CDS	45.0%
	GAACCTTCAATGGCTTCTCT+GGG	-	chr8_1:77241987-77242006	None:intergenic	45.0%
	CAATCGCAGGTTCATCATTC+AGG	-	chr8_1:77242373-77242392	None:intergenic	45.0%
	GACTATGGATCTTCAATCGC+AGG	-	chr8_1:77242386-77242405	None:intergenic	45.0%
	GTGTCATGGCATCATCTACT+TGG	+	chr8_1:77242770-77242789	Msa1215820:intron	45.0%
	ACACGATCAACGACAACAAG+TGG	-	chr8_1:77242809-77242828	None:intergenic	45.0%
	TGTTGTCGTTGATCGTGTCA+TGG	+	chr8_1:77242811-77242830	Msa1215820:intron	45.0%
	GTGTCATGGCATCATCTACT+TGG	+	chr8_1:77242770-77242789	Msa1215820:intron	45.0%
!	AGGGCACTTCTTGTCAATGT+AGG	-	chr8_1:77243026-77243045	None:intergenic	45.0%
!!!	GACAAGAAGTGCCCTTTTAC+TGG	+	chr8_1:77243031-77243050	Msa1215820:CDS	45.0%
	TACTGGCAATGTTTCCATCC+GGG	+	chr8_1:77243048-77243067	Msa1215820:CDS	45.0%
	CAATGCAGGTGAGACTCAAT+AGG	+	chr8_1:77243335-77243354	Msa1215820:intron	45.0%
	ATTGCTCCGGTCCAGTAAAA+TGG	+	chr8_1:77241514-77241533	Msa1215820:five_prime_UTR	45.0%
	ATTCCGAAGCTTCTTCACCA+TGG	+	chr8_1:77241628-77241647	Msa1215820:exon	45.0%
	AGCTCAAAGAAGAGTGGGAA+AGG	+	chr8_1:77241900-77241919	Msa1215820:intron	45.0%
	AGAAGAGTGGGAAAGGAAAG+AGG	+	chr8_1:77241907-77241926	Msa1215820:CDS	45.0%
	AAAGGAAAGAGGCCTGGAAA+AGG	+	chr8_1:77241918-77241937	Msa1215820:CDS	45.0%
	GAACCTTCAATGGCTTCTCT+GGG	-	chr8_1:77241987-77242006	None:intergenic	45.0%
	CAATCGCAGGTTCATCATTC+AGG	-	chr8_1:77242373-77242392	None:intergenic	45.0%
	GACTATGGATCTTCAATCGC+AGG	-	chr8_1:77242386-77242405	None:intergenic	45.0%
	GTGTCATGGCATCATCTACT+TGG	+	chr8_1:77242770-77242789	Msa1215820:intron	45.0%
	ACACGATCAACGACAACAAG+TGG	-	chr8_1:77242809-77242828	None:intergenic	45.0%
	TGTTGTCGTTGATCGTGTCA+TGG	+	chr8_1:77242811-77242830	Msa1215820:intron	45.0%
	GTGTCATGGCATCATCTACT+TGG	+	chr8_1:77242770-77242789	Msa1215820:intron	45.0%
!	AGGGCACTTCTTGTCAATGT+AGG	-	chr8_1:77243026-77243045	None:intergenic	45.0%
!!!	GACAAGAAGTGCCCTTTTAC+TGG	+	chr8_1:77243031-77243050	Msa1215820:CDS	45.0%
	TACTGGCAATGTTTCCATCC+GGG	+	chr8_1:77243048-77243067	Msa1215820:CDS	45.0%
	CAATGCAGGTGAGACTCAAT+AGG	+	chr8_1:77243335-77243354	Msa1215820:intron	45.0%
!!	AAAATAAAAAAAATCATAAA+AGG	-	chr8_1:77242992-77243011	None:intergenic	5.0%
!!	AAAATAAAAAAAATCATAAA+AGG	-	chr8_1:77242992-77243011	None:intergenic	5.0%
!!!	AGTGAGAGGGTTTTCGAGTG+CGG	+	chr8_1:77241575-77241594	Msa1215820:five_prime_UTR	50.0%
	GAAGCTTCGGAATTTCGCAG+AGG	-	chr8_1:77241621-77241640	None:intergenic	50.0%
	CAGCCATGGTGAAGAAGCTT+CGG	-	chr8_1:77241634-77241653	None:intergenic	50.0%
!	AAGCGGTTTCCACCTTTTCC+AGG	-	chr8_1:77241933-77241952	None:intergenic	50.0%
	ACTCCCAGAGAAGCCATTGA+AGG	+	chr8_1:77241981-77242000	Msa1215820:CDS	50.0%
	GGAACCTTCAATGGCTTCTC+TGG	-	chr8_1:77241988-77242007	None:intergenic	50.0%
	ACTGGCAATGTTTCCATCCG+GGG	+	chr8_1:77243049-77243068	Msa1215820:CDS	50.0%
	TTCCAGCTAAAATACGGCCC+CGG	-	chr8_1:77243069-77243088	None:intergenic	50.0%
	AAGCAGGGTGATACATGAGC+AGG	-	chr8_1:77243281-77243300	None:intergenic	50.0%
	ATCACCCTGCTTCCGTGTTA+AGG	+	chr8_1:77243289-77243308	Msa1215820:CDS	50.0%
	CCTTCCTTAACACGGAAGCA+GGG	-	chr8_1:77243296-77243315	None:intergenic	50.0%
	TCCTTCCTTAACACGGAAGC+AGG	-	chr8_1:77243297-77243316	None:intergenic	50.0%
	TGCAGGTGAGACTCAATAGG+AGG	+	chr8_1:77243338-77243357	Msa1215820:intron	50.0%
	TGGAGAGAGGCCTGATGAAA+TGG	-	chr8_1:77243467-77243486	None:intergenic	50.0%
	AACCTCACAGTCTTGGAGAG+AGG	-	chr8_1:77243480-77243499	None:intergenic	50.0%
	GACGTTGAACCTCACAGTCT+TGG	-	chr8_1:77243487-77243506	None:intergenic	50.0%
	GTCTTGAAAGTGATCCCAGC+TGG	+	chr8_1:77243504-77243523	Msa1215820:CDS	50.0%
!!	GCTGGGAAGAAGGCATTTAC+TGG	+	chr8_1:77243540-77243559	Msa1215820:CDS	50.0%
!!!	AGTGAGAGGGTTTTCGAGTG+CGG	+	chr8_1:77241575-77241594	Msa1215820:five_prime_UTR	50.0%
	GAAGCTTCGGAATTTCGCAG+AGG	-	chr8_1:77241621-77241640	None:intergenic	50.0%
	CAGCCATGGTGAAGAAGCTT+CGG	-	chr8_1:77241634-77241653	None:intergenic	50.0%
!	AAGCGGTTTCCACCTTTTCC+AGG	-	chr8_1:77241933-77241952	None:intergenic	50.0%
	ACTCCCAGAGAAGCCATTGA+AGG	+	chr8_1:77241981-77242000	Msa1215820:CDS	50.0%
	GGAACCTTCAATGGCTTCTC+TGG	-	chr8_1:77241988-77242007	None:intergenic	50.0%
	ACTGGCAATGTTTCCATCCG+GGG	+	chr8_1:77243049-77243068	Msa1215820:CDS	50.0%
	TTCCAGCTAAAATACGGCCC+CGG	-	chr8_1:77243069-77243088	None:intergenic	50.0%
	AAGCAGGGTGATACATGAGC+AGG	-	chr8_1:77243281-77243300	None:intergenic	50.0%
	ATCACCCTGCTTCCGTGTTA+AGG	+	chr8_1:77243289-77243308	Msa1215820:CDS	50.0%
	CCTTCCTTAACACGGAAGCA+GGG	-	chr8_1:77243296-77243315	None:intergenic	50.0%
	TCCTTCCTTAACACGGAAGC+AGG	-	chr8_1:77243297-77243316	None:intergenic	50.0%
	TGCAGGTGAGACTCAATAGG+AGG	+	chr8_1:77243338-77243357	Msa1215820:intron	50.0%
	TGGAGAGAGGCCTGATGAAA+TGG	-	chr8_1:77243467-77243486	None:intergenic	50.0%
	AACCTCACAGTCTTGGAGAG+AGG	-	chr8_1:77243480-77243499	None:intergenic	50.0%
	GACGTTGAACCTCACAGTCT+TGG	-	chr8_1:77243487-77243506	None:intergenic	50.0%
	GTCTTGAAAGTGATCCCAGC+TGG	+	chr8_1:77243504-77243523	Msa1215820:CDS	50.0%
!!	GCTGGGAAGAAGGCATTTAC+TGG	+	chr8_1:77243540-77243559	Msa1215820:CDS	50.0%
	AGTGGGAAAGGAAAGAGGCC+TGG	+	chr8_1:77241912-77241931	Msa1215820:CDS	55.0%
	GGAAAGAGGCCTGGAAAAGG+TGG	+	chr8_1:77241921-77241940	Msa1215820:CDS	55.0%
	AGCTAAAATACGGCCCCGGA+TGG	-	chr8_1:77243065-77243084	None:intergenic	55.0%
!!!	ATCCGGGGCCGTATTTTAGC+TGG	+	chr8_1:77243064-77243083	Msa1215820:CDS	55.0%
	CCCTGCTTCCGTGTTAAGGA+AGG	+	chr8_1:77243293-77243312	Msa1215820:CDS	55.0%
!	ATCCCAGCTGGTTCATCCAG+TGG	+	chr8_1:77243516-77243535	Msa1215820:CDS	55.0%
	CACCACTGGATGAACCAGCT+GGG	-	chr8_1:77243521-77243540	None:intergenic	55.0%
	CTGGTTCATCCAGTGGTGCT+GGG	+	chr8_1:77243523-77243542	Msa1215820:CDS	55.0%
!	ATCCAGTGGTGCTGGGAAGA+AGG	+	chr8_1:77243530-77243549	Msa1215820:CDS	55.0%
	AGTGGGAAAGGAAAGAGGCC+TGG	+	chr8_1:77241912-77241931	Msa1215820:CDS	55.0%
	GGAAAGAGGCCTGGAAAAGG+TGG	+	chr8_1:77241921-77241940	Msa1215820:CDS	55.0%
	AGCTAAAATACGGCCCCGGA+TGG	-	chr8_1:77243065-77243084	None:intergenic	55.0%
!!!	ATCCGGGGCCGTATTTTAGC+TGG	+	chr8_1:77243064-77243083	Msa1215820:CDS	55.0%
	CCCTGCTTCCGTGTTAAGGA+AGG	+	chr8_1:77243293-77243312	Msa1215820:CDS	55.0%
!	ATCCCAGCTGGTTCATCCAG+TGG	+	chr8_1:77243516-77243535	Msa1215820:CDS	55.0%
	CACCACTGGATGAACCAGCT+GGG	-	chr8_1:77243521-77243540	None:intergenic	55.0%
	CTGGTTCATCCAGTGGTGCT+GGG	+	chr8_1:77243523-77243542	Msa1215820:CDS	55.0%
!	ATCCAGTGGTGCTGGGAAGA+AGG	+	chr8_1:77243530-77243549	Msa1215820:CDS	55.0%
	GGCCTCTCTCCAAGACTGTG+AGG	+	chr8_1:77243475-77243494	Msa1215820:intron	60.0%
	GCACCACTGGATGAACCAGC+TGG	-	chr8_1:77243522-77243541	None:intergenic	60.0%
!	GCTGGTTCATCCAGTGGTGC+TGG	+	chr8_1:77243522-77243541	Msa1215820:CDS	60.0%
	TGCCTTCTTCCCAGCACCAC+TGG	-	chr8_1:77243535-77243554	None:intergenic	60.0%
	GGCCTCTCTCCAAGACTGTG+AGG	+	chr8_1:77243475-77243494	Msa1215820:intron	60.0%
	GCACCACTGGATGAACCAGC+TGG	-	chr8_1:77243522-77243541	None:intergenic	60.0%
!	GCTGGTTCATCCAGTGGTGC+TGG	+	chr8_1:77243522-77243541	Msa1215820:CDS	60.0%
	TGCCTTCTTCCCAGCACCAC+TGG	-	chr8_1:77243535-77243554	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	77241484	77243843	77241484	ID=Msa1215820;Name=Msa1215820
chr8_1	mRNA	77241484	77243843	77241484	ID=Msa1215820-mRNA-1;Parent=Msa1215820;Name=Msa1215820-mRNA-1;_AED=0.07;_eAED=0.07;_QI=163\|1\|1\|1\|0.83\|0.71\|7\|245\|160
chr8_1	exon	77241484	77241658	77241484	ID=Msa1215820-mRNA-1:exon:26619;Parent=Msa1215820-mRNA-1
chr8_1	exon	77241760	77241800	77241760	ID=Msa1215820-mRNA-1:exon:26620;Parent=Msa1215820-mRNA-1
chr8_1	exon	77241902	77242002	77241902	ID=Msa1215820-mRNA-1:exon:26621;Parent=Msa1215820-mRNA-1
chr8_1	exon	77243020	77243164	77243020	ID=Msa1215820-mRNA-1:exon:26622;Parent=Msa1215820-mRNA-1
chr8_1	exon	77243253	77243342	77243253	ID=Msa1215820-mRNA-1:exon:26623;Parent=Msa1215820-mRNA-1
chr8_1	exon	77243476	77243726	77243476	ID=Msa1215820-mRNA-1:exon:26624;Parent=Msa1215820-mRNA-1
chr8_1	exon	77243756	77243843	77243756	ID=Msa1215820-mRNA-1:exon:26625;Parent=Msa1215820-mRNA-1
chr8_1	five_prime_UTR	77241484	77241646	77241484	ID=Msa1215820-mRNA-1:five_prime_utr;Parent=Msa1215820-mRNA-1
chr8_1	CDS	77241647	77241658	77241647	ID=Msa1215820-mRNA-1:cds;Parent=Msa1215820-mRNA-1
chr8_1	CDS	77241760	77241800	77241760	ID=Msa1215820-mRNA-1:cds;Parent=Msa1215820-mRNA-1
chr8_1	CDS	77241902	77242002	77241902	ID=Msa1215820-mRNA-1:cds;Parent=Msa1215820-mRNA-1
chr8_1	CDS	77243020	77243164	77243020	ID=Msa1215820-mRNA-1:cds;Parent=Msa1215820-mRNA-1
chr8_1	CDS	77243253	77243342	77243253	ID=Msa1215820-mRNA-1:cds;Parent=Msa1215820-mRNA-1
chr8_1	CDS	77243476	77243569	77243476	ID=Msa1215820-mRNA-1:cds;Parent=Msa1215820-mRNA-1
chr8_1	three_prime_UTR	77243570	77243726	77243570	ID=Msa1215820-mRNA-1:three_prime_utr;Parent=Msa1215820-mRNA-1
chr8_1	three_prime_UTR	77243756	77243843	77243756	ID=Msa1215820-mRNA-1:three_prime_utr;Parent=Msa1215820-mRNA-1

Gene Sequence

>Msa1215820

ATGGCTGAACAAACTGAGAAGGCTTTTCTGAAGCAACCTAAAGTGTTTCTATGCTCAAAGAAGAGTGGGAAAGGAAAGAGGCCTGGAAAAGGTGGAAACCGCTTCTTTAAATCCGTTGGTCTTGGATTTAAGACTCCCAGAGAAGCCATTGAAGGAACCTACATTGACAAGAAGTGCCCTTTTACTGGCAATGTTTCCATCCGGGGCCGTATTTTAGCTGGAACTTGTCACAGTGCTAAGATGAATAGGACTATTATTGTTAGGAGGAATTATCTTCACTTTATCAAGAAGTATCAGAGATATGAGAAGAGGCACTCAAACATTCCTGCTCATGTATCACCCTGCTTCCGTGTTAAGGAAGGAGATCATGTTATTATTGGGCAATGCAGGCCTCTCTCCAAGACTGTGAGGTTCAACGTCTTGAAAGTGATCCCAGCTGGTTCATCCAGTGGTGCTGGGAAGAAGGCATTTACTGGAATGTGA

Protein sequence

>Msa1215820

MAEQTEKAFLKQPKVFLCSKKSGKGKRPGKGGNRFFKSVGLGFKTPREAIEGTYIDKKCPFTGNVSIRGRILAGTCHSAKMNRTIIVRRNYLHFIKKYQRYEKRHSNIPAHVSPCFRVKEGDHVIIGQCRPLSKTVRFNVLKVIPAGSSSGAGKKAFTGM*