AlfalfaGEDB | Msa0505470

M. sativa cultivar zhongmu-4 / Msa0505470

KEGG: K13423|K13424
GO: GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009700 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010035 GO:0010120 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010506 GO:0010508 GO:0010556 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0031323 GO:0031325 GO:0031326 GO:0031329 GO:0031331 GO:0033554 GO:0034605 GO:0034641 GO:0042221 GO:0042430 GO:0042435 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044424 GO:0044464 GO:0044550 GO:0046217 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0070370 GO:0071704 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0505470	AKM20827.1	97.656	512	10	2	1	511	1	511	0.0	1026

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0505470	sp\|Q6B6R4\|WRK24_ORYSI	55.668	397	133	11	62	427	65	449	2.62e-130	393

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0505470	A0A0G3Y2B1	97.656	512	10	2	1	511	1	511	0.0	1026

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TFs/TRs:

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Gene ID	Type	Classification
Msa0505470	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0238710	Msa0505470	0.821533	3.838522e-53	-8.615850e-47
Msa0264680	Msa0505470	0.823297	1.494110e-53	-8.615850e-47
Msa0302730	Msa0505470	0.803603	3.222416e-49	-8.615850e-47
Msa0306310	Msa0505470	0.802866	4.577226e-49	-8.615850e-47
Msa0316470	Msa0505470	0.805368	1.380983e-49	-8.615850e-47
Msa0393100	Msa0505470	0.805026	1.628390e-49	-8.615850e-47
Msa0505470	Msa0645480	0.800875	1.173876e-48	-8.615850e-47
Msa0505470	Msa0734940	0.801462	8.902561e-49	-8.615850e-47
Msa0505470	Msa0781210	0.815331	9.766224e-52	-8.615850e-47
Msa0505470	Msa0804870	0.806772	6.993145e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0505470	MtrunA17_Chr3g0106491	93.177	513	28	2	1	511	1	508	0.0	920
Msa0505470	MtrunA17_Chr5g0432161	57.143	567	166	18	1	510	35	581	2.66e-164	478
Msa0505470	MtrunA17_Chr7g0243891	47.818	527	200	21	39	511	37	542	1.30e-125	377
Msa0505470	MtrunA17_Chr4g0004911	46.300	473	201	14	61	510	47	489	8.29e-107	327
Msa0505470	MtrunA17_Chr5g0445311	44.313	422	180	11	40	427	28	428	2.16e-94	297
Msa0505470	MtrunA17_Chr3g0101131	43.213	442	184	13	40	425	47	477	1.64e-91	291
Msa0505470	MtrunA17_Chr3g0101131	41.463	123	62	4	183	300	406	523	6.35e-17	84.0
Msa0505470	MtrunA17_Chr5g0418191	50.360	278	129	5	185	457	140	413	2.32e-81	259
Msa0505470	MtrunA17_Chr7g0218081	40.288	417	190	11	41	431	95	478	1.15e-80	260
Msa0505470	MtrunA17_Chr7g0218081	47.727	88	41	2	183	269	401	484	1.57e-15	79.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0505470	AT2G38470.1	43.728	558	226	19	1	510	1	518	1.53e-111	341
Msa0505470	AT5G07100.1	48.361	366	90	10	62	425	28	296	5.22e-94	288
Msa0505470	AT2G03340.1	45.729	398	181	10	39	427	108	479	1.93e-92	291
Msa0505470	AT2G03340.1	60.000	70	27	1	363	432	250	318	1.47e-19	92.0
Msa0505470	AT4G26640.7	44.186	430	172	12	51	432	1	410	1.13e-90	287
Msa0505470	AT4G26640.2	43.023	430	192	11	40	432	37	450	1.61e-90	288
Msa0505470	AT2G30250.1	45.330	439	173	15	1	423	1	388	9.74e-90	280
Msa0505470	AT2G30250.1	55.385	65	28	1	197	260	329	393	9.15e-16	79.7
Msa0505470	AT4G26640.5	56.911	246	93	4	195	432	34	274	1.90e-86	271
Msa0505470	AT4G26640.6	56.911	246	93	4	195	432	34	274	1.90e-86	271

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Find 119 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAATAAATTGAAGGGT+TGG	0.397409	3_4:+50976872	None:intergenic
AAAGTTGAGAGGGGTTTAGA+CGG	0.602739	3_4:-50975212	Msa0505470:CDS
AAATGAAATTCAAATGGATT+TGG	0.360393	3_4:-50975060	Msa0505470:CDS
AACAATGTTTCACTTCATAA+TGG	0.317177	3_4:-50973227	Msa0505470:CDS
AAGAGAGGTGCAGGAGAAAT+AGG	0.210851	3_4:+50976632	None:intergenic
AAGGAACATTCCTTCAAGAT+AGG	0.432156	3_4:+50973063	None:intergenic
AAGGAGGTCAGAGAAGAAAG+AGG	0.512892	3_4:+50976772	None:intergenic
AAGTGTTGTCGGATCAAAAC+AGG	0.453220	3_4:-50976700	Msa0505470:CDS
AATGATCCGGTAGTTGGAGA+TGG	0.563554	3_4:+50975905	None:intergenic
AATGGATTTGGTTGCTACAC+CGG	0.505910	3_4:-50975048	Msa0505470:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGTCATTGAATTATAGAGA+AGG	+	chr3_4:50973222-50973241	None:intergenic	30.0%
TATGTCTATGTGATTCTAGT+TGG	-	chr3_4:50974044-50974063	Msa0505470:intron	30.0%
CTAATAATGAAGGCTTCAAT+TGG	-	chr3_4:50974134-50974153	Msa0505470:intron	30.0%
AAACTTTCATGTATACCAAC+AGG	-	chr3_4:50974519-50974538	Msa0505470:intron	30.0%
AACTTTCATGTATACCAACA+GGG	-	chr3_4:50974520-50974539	Msa0505470:intron	30.0%
GGATACAATTGGAGAAAATA+TGG	-	chr3_4:50974703-50974722	Msa0505470:CDS	30.0%
CAACAAAGAAAAAAGTTGAG+AGG	-	chr3_4:50974791-50974810	Msa0505470:intron	30.0%
ACAAAGAAAAAAGTTGAGAG+GGG	-	chr3_4:50974793-50974812	Msa0505470:intron	30.0%
TTTCCAAAATGATTTGAAGG+AGG	+	chr3_4:50974937-50974956	None:intergenic	30.0%
TAATATTGCAGGAGAATAGA+AGG	-	chr3_4:50975279-50975298	Msa0505470:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	50973076	50976960	50973076	ID=Msa0505470;Name=Msa0505470
chr3_4	mRNA	50973076	50976960	50973076	ID=Msa0505470-mRNA-1;Parent=Msa0505470;Name=Msa0505470-mRNA-1;_AED=0.01;_eAED=0.01;_QI=159\|1\|0.8\|1\|1\|1\|5\|0\|511
chr3_4	exon	50973076	50973463	50973076	ID=Msa0505470-mRNA-1:exon:11083;Parent=Msa0505470-mRNA-1
chr3_4	exon	50974582	50974746	50974582	ID=Msa0505470-mRNA-1:exon:11082;Parent=Msa0505470-mRNA-1
chr3_4	exon	50974942	50975495	50974942	ID=Msa0505470-mRNA-1:exon:11081;Parent=Msa0505470-mRNA-1
chr3_4	exon	50975760	50975933	50975760	ID=Msa0505470-mRNA-1:exon:11080;Parent=Msa0505470-mRNA-1
chr3_4	exon	50976547	50976960	50976547	ID=Msa0505470-mRNA-1:exon:11079;Parent=Msa0505470-mRNA-1
chr3_4	five_prime_UTR	50976802	50976960	50976802	ID=Msa0505470-mRNA-1:five_prime_utr;Parent=Msa0505470-mRNA-1
chr3_4	CDS	50976547	50976801	50976547	ID=Msa0505470-mRNA-1:cds;Parent=Msa0505470-mRNA-1
chr3_4	CDS	50975760	50975933	50975760	ID=Msa0505470-mRNA-1:cds;Parent=Msa0505470-mRNA-1
chr3_4	CDS	50974942	50975495	50974942	ID=Msa0505470-mRNA-1:cds;Parent=Msa0505470-mRNA-1
chr3_4	CDS	50974582	50974746	50974582	ID=Msa0505470-mRNA-1:cds;Parent=Msa0505470-mRNA-1
chr3_4	CDS	50973076	50973463	50973076	ID=Msa0505470-mRNA-1:cds;Parent=Msa0505470-mRNA-1

Gene Sequence

>Msa0505470

ATGACCTCCTCTTTCTTCTCTGACCTCCTTAACTCTCCAACCGACCAAAACCTTAAAGACACCGAAAGATCTCATCATCAAGTGTTGTCGGATCAAAACAGGTCTGGTTTACCCCCCAAATTCAAGTCTACACCTCCTCCATCTCTTCCTATTTCTCCTGCACCTCTCTTTTCTCCTTCTTCATACTTTTCTATACCTCCTGGCTTCTCTCTTTCTGAACTTCTTGATTCTCCTGTTCTCCTTAACTCTTCTCATATTCTTCCATCTCCAACTACCGGATCATTTGCTAATAATGAAGGCTTCAATTGGAAGAATAATTATGAGGAAAAGCAAGAGAATGTCAAGGCAGAAGAGGAAACCATCTCATCAAGTTTCTCCTTCCAAGCTCAACCAGCTGGGTTTCAATCTTCGACCACCACAGTTCAAAATGGATGGAACATGGTGAAAACTGAAAACTCTTCTTCAATGCAAAGTTTCCCACCAGAGAGTAATTCTACTAGTGAACAAAACAACAAGAGTGGTTCACAATCAAATTACAACAATTATCAATCACAGCCACAAGTTCAAATCCTTAGTAGAAGATCAGATGATGGATACAATTGGAGAAAATATGGACAGAAACAAGTGAAAGGAAGTGAAAATCCAAGAAGCTATTACAAATGCACATACCCTAATTGTCCAACAAAGAAAAAAGTTGAGAGGGGTTTAGACGGACAAATTACTGAGATAGTTTACAAAGGTAGTCATAATCATCCTAAGCCTGTGGCTAACAAGAGGAACATAAACTCGATGTCTTCATCGTCGTCGTCACTTGCAAATCCTCCTCCTTCAAATCATTTTGGAAATGAAATTCAAATGGATTTGGTTGCTACACCGGAGAATTCATCGATATCGATTGGAGATGATGAATTTGAGCAGACTTCTCATAAATCTGGTGGAGATCATGATCAGTTTTGTGAAGATGAACCTGATGCCAAAAAATGGAGAATAGAAGGTGAAAATGAGGGAATATCATTAGGAGTTGGAAATAGAACTGTGAGGGAACCAAGAGTTGTTGTTCAAACAACAAGTGACATCGATATTCTAGATGATGGATACAGATGGAGGAAATATGGGCAGAAAGTAGTAAAGGGAAATCCAAATCCAAGGAGTTACTACAAGTGCACAAACCCAGGATGTCCAGTGAGAAAACATGTCGAACGAGCCTCACAAGACCTAAGAGCAGTGATAACAACGTATGAAGGTAAACACAATCATGATGTTCCAGCACCACGTGGAAGTGGAAACCATTCTATCAACAAACCACTACCAATGCAAACAACTATGAACAATACCACAAGTGCCAACAATTCCATCAACAACAACAATGTTTCACTTCATAATGGATTCTTCAACCATGAGATGATGCAGAACAATGGATTCACGGTTGATTCTTACATGAATATGAATCAACAACGTGTTAATAATATTCTTAGAGGTAGAGCCAAGGAGGAGCCTGTAGAAGATGACTCATTTTTTGAATCTTTCCTATCTTGA

Protein sequence

>Msa0505470

MTSSFFSDLLNSPTDQNLKDTERSHHQVLSDQNRSGLPPKFKSTPPPSLPISPAPLFSPSSYFSIPPGFSLSELLDSPVLLNSSHILPSPTTGSFANNEGFNWKNNYEEKQENVKAEEETISSSFSFQAQPAGFQSSTTTVQNGWNMVKTENSSSMQSFPPESNSTSEQNNKSGSQSNYNNYQSQPQVQILSRRSDDGYNWRKYGQKQVKGSENPRSYYKCTYPNCPTKKKVERGLDGQITEIVYKGSHNHPKPVANKRNINSMSSSSSSLANPPPSNHFGNEIQMDLVATPENSSISIGDDEFEQTSHKSGGDHDQFCEDEPDAKKWRIEGENEGISLGVGNRTVREPRVVVQTTSDIDILDDGYRWRKYGQKVVKGNPNPRSYYKCTNPGCPVRKHVERASQDLRAVITTYEGKHNHDVPAPRGSGNHSINKPLPMQTTMNNTTSANNSINNNNVSLHNGFFNHEMMQNNGFTVDSYMNMNQQRVNNILRGRAKEEPVEDDSFFESFLS*