AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0512420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0512420 MtrunA17_Chr3g0120531 86.957 115 14 1 1 114 1 115 1.74e-59 178
Msa0512420 MtrunA17_Chr3g0120541 71.429 119 25 3 1 114 1 115 1.40e-49 153
Msa0512420 MtrunA17_Chr3g0120551 59.664 119 41 3 1 114 1 117 1.42e-38 125
Msa0512420 MtrunA17_Chr2g0290831 52.703 74 35 0 3 76 2 75 8.14e-21 79.3
Msa0512420 MtrunA17_Chr5g0438551 43.609 133 51 5 4 113 2 133 1.52e-20 80.5
Msa0512420 MtrunA17_Chr6g0483521 51.613 62 30 0 3 64 2 63 2.58e-16 71.6
Msa0512420 MtrunA17_Chr6g0483511 51.613 62 30 0 3 64 2 63 2.39e-15 68.2
Msa0512420 MtrunA17_Chr6g0483581 46.575 73 39 0 3 75 2 74 5.20e-15 68.2
Msa0512420 MtrunA17_Chr6g0483561 45.714 70 38 0 3 72 2 71 2.57e-14 66.2
Msa0512420 MtrunA17_Chr6g0469651 48.571 70 33 2 4 73 2 68 1.01e-11 57.8
Msa0512420 MtrunA17_Chr6g0469571 44.928 69 35 2 5 73 3 68 3.89e-11 55.8
Msa0512420 MtrunA17_Chr6g0469551 44.928 69 35 2 5 73 3 68 4.87e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0512420 AT3G20180.3 43.617 94 52 1 3 96 2 94 2.64e-18 74.7
Msa0512420 AT5G48290.1 50.602 83 38 1 3 82 2 84 4.81e-18 75.5
Msa0512420 AT3G20180.2 42.553 94 53 1 3 96 21 113 1.13e-17 73.6
Msa0512420 AT3G07600.1 46.154 78 42 0 3 80 2 79 1.71e-17 73.6
Msa0512420 AT3G20180.4 42.391 92 52 1 5 96 2 92 5.43e-17 71.2
Msa0512420 AT3G20180.1 42.697 89 50 1 8 96 3 90 1.12e-16 70.5
Msa0512420 AT5G48290.3 41.748 103 37 2 3 82 2 104 2.66e-14 66.2
Msa0512420 AT5G48290.2 48.649 74 35 1 12 82 1 74 1.72e-13 63.5
Msa0512420 AT1G55775.1 45.070 71 38 1 2 72 3 72 4.28e-13 61.6
Msa0512420 AT4G05030.2 45.333 75 40 1 1 75 1 74 1.14e-12 58.9
Msa0512420 AT4G05030.1 43.056 72 41 0 4 75 36 107 6.64e-12 57.8

Find 29 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATTGCAGTTGGAGTTTC+AGG 0.157536 3_4:+62178044 Msa0512420:CDS
TTTATATCTCATAAGTATTA+TGG 0.216328 3_4:+62177570 Msa0512420:exon
ACTTATGAGATATAAATAAA+TGG 0.288883 3_4:-62177563 None:intergenic
ATTAGAAGAAGCTTTGTTTC+CGG 0.305912 3_4:-62177540 None:intergenic
AACTGCAATCTTCATGGCTT+TGG 0.309449 3_4:-62178033 None:intergenic
ACACAAGGCCATGCCATAAT+TGG 0.314736 3_4:-62178334 None:intergenic
AAAGAAAGAAGAACCAATTA+TGG 0.328215 3_4:+62178321 Msa0512420:CDS
AACTCCAACTGCAATCTTCA+TGG 0.358381 3_4:-62178039 None:intergenic
GAAATTCTGTCATGCAGAAT+TGG 0.360093 3_4:+62178267 Msa0512420:CDS
AAGGATCAAATAGAAGTAAT+CGG 0.441308 3_4:+62178202 Msa0512420:CDS
ACTTTAATAATACACCATTC+CGG 0.451555 3_4:+62177521 None:intergenic
ATGGCATGGCCTTGTGTAGC+TGG 0.465320 3_4:+62178340 Msa0512420:CDS
CTTATCTCACACACAGGGAC+AGG 0.478978 3_4:-62178376 None:intergenic
CTGTCCCTGTGTGTGAGATA+AGG 0.485521 3_4:+62178377 Msa0512420:CDS
TGTCCCTGTGTGTGAGATAA+GGG 0.495058 3_4:+62178378 Msa0512420:CDS
TCTCATAAGTATTATGGCTA+AGG 0.519265 3_4:+62177576 Msa0512420:exon
TCTTGATGACATTCACATGA+TGG 0.521926 3_4:-62178416 None:intergenic
GTGGAAAGTGCAGCAATACA+AGG 0.533044 3_4:+62178172 Msa0512420:CDS
GCAGCAAAAGATAGTGATCA+AGG 0.536634 3_4:+62177983 Msa0512420:intron
AAGAAGCTTTGTTTCCGGAA+TGG 0.538643 3_4:-62177535 None:intergenic
CACACAGGGACAGGGTAATG+AGG 0.564551 3_4:-62178367 None:intergenic
AAAGCCATGAAGATTGCAGT+TGG 0.583776 3_4:+62178035 Msa0512420:CDS
CATGCAGAATTGGTGAGTGT+TGG 0.620675 3_4:+62178277 Msa0512420:CDS
AGCAATACAAGGAGATAGCA+AGG 0.620845 3_4:+62178183 Msa0512420:CDS
AAGAAGAACCAATTATGGCA+TGG 0.625011 3_4:+62178326 Msa0512420:CDS
TCGTCCCTTATCTCACACAC+AGG 0.651929 3_4:-62178382 None:intergenic
TTATCTCACACACAGGGACA+GGG 0.661020 3_4:-62178375 None:intergenic
TGAGGATAACCAGCTACACA+AGG 0.666785 3_4:-62178349 None:intergenic
CGTCCCTTATCTCACACACA+GGG 0.689018 3_4:-62178381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTATATATATTGAATAA+AGG - chr3_4:62177650-62177669 None:intergenic 10.0%
!! ATAGTATATATATTGAATAA+AGG - chr3_4:62177650-62177669 None:intergenic 10.0%
!! ACTTATGAGATATAAATAAA+TGG - chr3_4:62177566-62177585 None:intergenic 15.0%
!! TTTATATCTCATAAGTATTA+TGG + chr3_4:62177570-62177589 Msa0512420:exon 15.0%
!!! TAATCCTTTTTTGAAATTTT+TGG - chr3_4:62177857-62177876 None:intergenic 15.0%
!! ACTTATGAGATATAAATAAA+TGG - chr3_4:62177566-62177585 None:intergenic 15.0%
!! TTTATATCTCATAAGTATTA+TGG + chr3_4:62177570-62177589 Msa0512420:exon 15.0%
!!! TAATCCTTTTTTGAAATTTT+TGG - chr3_4:62177857-62177876 None:intergenic 15.0%
!! GTATATATATTGAATAAAGG+AGG - chr3_4:62177647-62177666 None:intergenic 20.0%
!! GTATATATATTGAATAAAGG+AGG - chr3_4:62177647-62177666 None:intergenic 20.0%
! TCCACCAAAAATTTCAAAAA+AGG + chr3_4:62177850-62177869 Msa0512420:intron 25.0%
!!! TCCTTTTTTGAAATTTTTGG+TGG - chr3_4:62177854-62177873 None:intergenic 25.0%
! TAGTTTGGATAATTAAGCTA+TGG - chr3_4:62178129-62178148 None:intergenic 25.0%
!!! ATTTTTGGACATGATTAGTT+TGG - chr3_4:62178144-62178163 None:intergenic 25.0%
! AATCATGTCCAAAAATATTC+AGG + chr3_4:62178148-62178167 Msa0512420:intron 25.0%
! AAGGATCAAATAGAAGTAAT+CGG + chr3_4:62178202-62178221 Msa0512420:CDS 25.0%
! AAAGAAAGAAGAACCAATTA+TGG + chr3_4:62178321-62178340 Msa0512420:CDS 25.0%
! TCCACCAAAAATTTCAAAAA+AGG + chr3_4:62177850-62177869 Msa0512420:intron 25.0%
!!! TCCTTTTTTGAAATTTTTGG+TGG - chr3_4:62177854-62177873 None:intergenic 25.0%
! TAGTTTGGATAATTAAGCTA+TGG - chr3_4:62178129-62178148 None:intergenic 25.0%
!!! ATTTTTGGACATGATTAGTT+TGG - chr3_4:62178144-62178163 None:intergenic 25.0%
! AATCATGTCCAAAAATATTC+AGG + chr3_4:62178148-62178167 Msa0512420:intron 25.0%
! AAGGATCAAATAGAAGTAAT+CGG + chr3_4:62178202-62178221 Msa0512420:CDS 25.0%
! AAAGAAAGAAGAACCAATTA+TGG + chr3_4:62178321-62178340 Msa0512420:CDS 25.0%
!! ATTAGAAGAAGCTTTGTTTC+CGG - chr3_4:62177543-62177562 None:intergenic 30.0%
TCTCATAAGTATTATGGCTA+AGG + chr3_4:62177576-62177595 Msa0512420:exon 30.0%
!!! TCACACTACTTTTTTTTAGC+AGG - chr3_4:62177619-62177638 None:intergenic 30.0%
TATATTGAATAAAGGAGGTC+AGG - chr3_4:62177642-62177661 None:intergenic 30.0%
! TTTCCACTCCTGAATATTTT+TGG - chr3_4:62178159-62178178 None:intergenic 30.0%
!! ATTAGAAGAAGCTTTGTTTC+CGG - chr3_4:62177543-62177562 None:intergenic 30.0%
TCTCATAAGTATTATGGCTA+AGG + chr3_4:62177576-62177595 Msa0512420:exon 30.0%
!!! TCACACTACTTTTTTTTAGC+AGG - chr3_4:62177619-62177638 None:intergenic 30.0%
TATATTGAATAAAGGAGGTC+AGG - chr3_4:62177642-62177661 None:intergenic 30.0%
! TTTCCACTCCTGAATATTTT+TGG - chr3_4:62178159-62178178 None:intergenic 30.0%
ATCCTTTGCTTGAAATTGAG+AGG + chr3_4:62177728-62177747 Msa0512420:intron 35.0%
TCCTTTGCTTGAAATTGAGA+GGG + chr3_4:62177729-62177748 Msa0512420:intron 35.0%
TTGAAATTGAGAGGGAAAGA+GGG + chr3_4:62177737-62177756 Msa0512420:intron 35.0%
TGTCCAAAAATATTCAGGAG+TGG + chr3_4:62178153-62178172 Msa0512420:intron 35.0%
GAAATTCTGTCATGCAGAAT+TGG + chr3_4:62178267-62178286 Msa0512420:CDS 35.0%
TGGTGAAGTGAAAAAGAAAG+AGG + chr3_4:62178297-62178316 Msa0512420:CDS 35.0%
AAGAAGAACCAATTATGGCA+TGG + chr3_4:62178326-62178345 Msa0512420:CDS 35.0%
ATCCTTTGCTTGAAATTGAG+AGG + chr3_4:62177728-62177747 Msa0512420:intron 35.0%
TCCTTTGCTTGAAATTGAGA+GGG + chr3_4:62177729-62177748 Msa0512420:intron 35.0%
TTGAAATTGAGAGGGAAAGA+GGG + chr3_4:62177737-62177756 Msa0512420:intron 35.0%
TGTCCAAAAATATTCAGGAG+TGG + chr3_4:62178153-62178172 Msa0512420:intron 35.0%
GAAATTCTGTCATGCAGAAT+TGG + chr3_4:62178267-62178286 Msa0512420:CDS 35.0%
TGGTGAAGTGAAAAAGAAAG+AGG + chr3_4:62178297-62178316 Msa0512420:CDS 35.0%
AAGAAGAACCAATTATGGCA+TGG + chr3_4:62178326-62178345 Msa0512420:CDS 35.0%
!! AAGAAGCTTTGTTTCCGGAA+TGG - chr3_4:62177538-62177557 None:intergenic 40.0%
TCCCTCTCAATTTCAAGCAA+AGG - chr3_4:62177733-62177752 None:intergenic 40.0%
CTTGAAATTGAGAGGGAAAG+AGG + chr3_4:62177736-62177755 Msa0512420:intron 40.0%
GCAGCAAAAGATAGTGATCA+AGG + chr3_4:62177983-62178002 Msa0512420:intron 40.0%
CAATGCACAGCCAAAAATCA+AGG + chr3_4:62178009-62178028 Msa0512420:CDS 40.0%
!!! TGGCTTTGGACCTTGATTTT+TGG - chr3_4:62178022-62178041 None:intergenic 40.0%
AACTGCAATCTTCATGGCTT+TGG - chr3_4:62178036-62178055 None:intergenic 40.0%
AAAGCCATGAAGATTGCAGT+TGG + chr3_4:62178035-62178054 Msa0512420:CDS 40.0%
AACTCCAACTGCAATCTTCA+TGG - chr3_4:62178042-62178061 None:intergenic 40.0%
AAGATTGCAGTTGGAGTTTC+AGG + chr3_4:62178044-62178063 Msa0512420:CDS 40.0%
AGCAATACAAGGAGATAGCA+AGG + chr3_4:62178183-62178202 Msa0512420:CDS 40.0%
!! AAGAAGCTTTGTTTCCGGAA+TGG - chr3_4:62177538-62177557 None:intergenic 40.0%
TCCCTCTCAATTTCAAGCAA+AGG - chr3_4:62177733-62177752 None:intergenic 40.0%
CTTGAAATTGAGAGGGAAAG+AGG + chr3_4:62177736-62177755 Msa0512420:intron 40.0%
GCAGCAAAAGATAGTGATCA+AGG + chr3_4:62177983-62178002 Msa0512420:intron 40.0%
CAATGCACAGCCAAAAATCA+AGG + chr3_4:62178009-62178028 Msa0512420:CDS 40.0%
!!! TGGCTTTGGACCTTGATTTT+TGG - chr3_4:62178022-62178041 None:intergenic 40.0%
AACTGCAATCTTCATGGCTT+TGG - chr3_4:62178036-62178055 None:intergenic 40.0%
AAAGCCATGAAGATTGCAGT+TGG + chr3_4:62178035-62178054 Msa0512420:CDS 40.0%
AACTCCAACTGCAATCTTCA+TGG - chr3_4:62178042-62178061 None:intergenic 40.0%
AAGATTGCAGTTGGAGTTTC+AGG + chr3_4:62178044-62178063 Msa0512420:CDS 40.0%
AGCAATACAAGGAGATAGCA+AGG + chr3_4:62178183-62178202 Msa0512420:CDS 40.0%
! GTGGAAAGTGCAGCAATACA+AGG + chr3_4:62178172-62178191 Msa0512420:CDS 45.0%
CATGCAGAATTGGTGAGTGT+TGG + chr3_4:62178277-62178296 Msa0512420:CDS 45.0%
ACACAAGGCCATGCCATAAT+TGG - chr3_4:62178337-62178356 None:intergenic 45.0%
TGAGGATAACCAGCTACACA+AGG - chr3_4:62178352-62178371 None:intergenic 45.0%
TTATCTCACACACAGGGACA+GGG - chr3_4:62178378-62178397 None:intergenic 45.0%
TGTCCCTGTGTGTGAGATAA+GGG + chr3_4:62178378-62178397 Msa0512420:CDS 45.0%
! GTGGAAAGTGCAGCAATACA+AGG + chr3_4:62178172-62178191 Msa0512420:CDS 45.0%
CATGCAGAATTGGTGAGTGT+TGG + chr3_4:62178277-62178296 Msa0512420:CDS 45.0%
ACACAAGGCCATGCCATAAT+TGG - chr3_4:62178337-62178356 None:intergenic 45.0%
TGAGGATAACCAGCTACACA+AGG - chr3_4:62178352-62178371 None:intergenic 45.0%
TTATCTCACACACAGGGACA+GGG - chr3_4:62178378-62178397 None:intergenic 45.0%
TGTCCCTGTGTGTGAGATAA+GGG + chr3_4:62178378-62178397 Msa0512420:CDS 45.0%
!! TTTGAATTAAAAATAATTAA+TGG - chr3_4:62178094-62178113 None:intergenic 5.0%
!! TTTGAATTAAAAATAATTAA+TGG - chr3_4:62178094-62178113 None:intergenic 5.0%
CTTATCTCACACACAGGGAC+AGG - chr3_4:62178379-62178398 None:intergenic 50.0%
CTGTCCCTGTGTGTGAGATA+AGG + chr3_4:62178377-62178396 Msa0512420:CDS 50.0%
CGTCCCTTATCTCACACACA+GGG - chr3_4:62178384-62178403 None:intergenic 50.0%
TCGTCCCTTATCTCACACAC+AGG - chr3_4:62178385-62178404 None:intergenic 50.0%
CTTATCTCACACACAGGGAC+AGG - chr3_4:62178379-62178398 None:intergenic 50.0%
CTGTCCCTGTGTGTGAGATA+AGG + chr3_4:62178377-62178396 Msa0512420:CDS 50.0%
CGTCCCTTATCTCACACACA+GGG - chr3_4:62178384-62178403 None:intergenic 50.0%
TCGTCCCTTATCTCACACAC+AGG - chr3_4:62178385-62178404 None:intergenic 50.0%
!! ATGGCATGGCCTTGTGTAGC+TGG + chr3_4:62178340-62178359 Msa0512420:CDS 55.0%
CACACAGGGACAGGGTAATG+AGG - chr3_4:62178370-62178389 None:intergenic 55.0%
!! ATGGCATGGCCTTGTGTAGC+TGG + chr3_4:62178340-62178359 Msa0512420:CDS 55.0%
CACACAGGGACAGGGTAATG+AGG - chr3_4:62178370-62178389 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 62177526 62178426 62177526 ID=Msa0512420;Name=Msa0512420
chr3_4 mRNA 62177526 62178426 62177526 ID=Msa0512420-mRNA-1;Parent=Msa0512420;Name=Msa0512420-mRNA-1;_AED=0.41;_eAED=0.41;_QI=63|1|0.66|1|1|1|3|0|114
chr3_4 exon 62177526 62177597 62177526 ID=Msa0512420-mRNA-1:exon:15385;Parent=Msa0512420-mRNA-1
chr3_4 exon 62177987 62178065 62177987 ID=Msa0512420-mRNA-1:exon:15386;Parent=Msa0512420-mRNA-1
chr3_4 exon 62178170 62178426 62178170 ID=Msa0512420-mRNA-1:exon:15387;Parent=Msa0512420-mRNA-1
chr3_4 five_prime_UTR 62177526 62177588 62177526 ID=Msa0512420-mRNA-1:five_prime_utr;Parent=Msa0512420-mRNA-1
chr3_4 CDS 62177589 62177597 62177589 ID=Msa0512420-mRNA-1:cds;Parent=Msa0512420-mRNA-1
chr3_4 CDS 62177987 62178065 62177987 ID=Msa0512420-mRNA-1:cds;Parent=Msa0512420-mRNA-1
chr3_4 CDS 62178170 62178426 62178170 ID=Msa0512420-mRNA-1:cds;Parent=Msa0512420-mRNA-1
Gene Sequence

>Msa0512420

ATGGCTAAGCAAAAGATAGTGATCAAGGTGTCAATGCACAGCCAAAAATCAAGGTCCAAAGCCATGAAGATTGCAGTTGGAGTTTCAGGAGTGGAAAGTGCAGCAATACAAGGAGATAGCAAGGATCAAATAGAAGTAATCGGAGAACAAATAGACTCAGTTAGACTCACAAAACTGCTGAGAAAGAAATTCTGTCATGCAGAATTGGTGAGTGTTGGTGAAGTGAAAAAGAAAGAGGAAAAGAAAGAAGAACCAATTATGGCATGGCCTTGTGTAGCTGGTTATCCTCATTACCCTGTCCCTGTGTGTGAGATAAGGGACGACTCTTCTTGTTCCATCATGTGA

Protein sequence

>Msa0512420

MAKQKIVIKVSMHSQKSRSKAMKIAVGVSGVESAAIQGDSKDQIEVIGEQIDSVRLTKLLRKKFCHAELVSVGEVKKKEEKKEEPIMAWPCVAGYPHYPVPVCEIRDDSSCSIM*