Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1198090 | KEH20113.1 | 82.609 | 92 | 15 | 1 | 1 | 91 | 1 | 92 | 2.49e-36 | 128 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0007010 | Msa1198090 | 0.806049 | 9.934217e-50 | -8.615850e-47 |
| Msa0009610 | Msa1198090 | 0.855012 | 8.394085e-62 | -8.615850e-47 |
| Msa0033340 | Msa1198090 | 0.806882 | 6.629374e-50 | -8.615850e-47 |
| Msa0055730 | Msa1198090 | 0.824426 | 8.122591e-54 | -8.615850e-47 |
| Msa0109970 | Msa1198090 | 0.810368 | 1.193318e-50 | -8.615850e-47 |
| Msa0629850 | Msa1198090 | 0.828353 | 9.423710e-55 | -8.615850e-47 |
| Msa0629930 | Msa1198090 | 0.805688 | 1.183261e-49 | -8.615850e-47 |
| Msa0629940 | Msa1198090 | 0.825513 | 4.499394e-54 | -8.615850e-47 |
| Msa0641900 | Msa1198090 | 0.820419 | 6.929105e-53 | -8.615850e-47 |
| Msa0682530 | Msa1198090 | 0.800203 | 1.609122e-48 | -8.615850e-47 |
| Msa0734800 | Msa1198090 | 0.816174 | 6.336725e-52 | -8.615850e-47 |
| Msa0743640 | Msa1198090 | 0.811832 | 5.747356e-51 | -8.615850e-47 |
| Msa0754180 | Msa1198090 | 0.817073 | 3.984636e-52 | -8.615850e-47 |
| Msa0776720 | Msa1198090 | 0.802793 | 4.738739e-49 | -8.615850e-47 |
| Msa0784520 | Msa1198090 | 0.803819 | 2.906050e-49 | -8.615850e-47 |
| Msa0784580 | Msa1198090 | 0.834101 | 3.642184e-56 | -8.615850e-47 |
| Msa0813950 | Msa1198090 | 0.821378 | 4.167751e-53 | -8.615850e-47 |
| Msa0815320 | Msa1198090 | 0.818949 | 1.500998e-52 | -8.615850e-47 |
| Msa0818010 | Msa1198090 | 0.839367 | 1.652890e-57 | -8.615850e-47 |
| Msa0818030 | Msa1198090 | 0.806875 | 6.651279e-50 | -8.615850e-47 |
| Msa0142230 | Msa1198090 | 0.839190 | 1.837248e-57 | -8.615850e-47 |
| Msa0149170 | Msa1198090 | 0.820703 | 5.962500e-53 | -8.615850e-47 |
| Msa0153430 | Msa1198090 | 0.809980 | 1.447018e-50 | -8.615850e-47 |
| Msa0156170 | Msa1198090 | 0.804672 | 1.930379e-49 | -8.615850e-47 |
| Msa0193010 | Msa1198090 | 0.824734 | 6.873164e-54 | -8.615850e-47 |
| Msa0235190 | Msa1198090 | 0.835720 | 1.423769e-56 | -8.615850e-47 |
| Msa0371810 | Msa1198090 | 0.812764 | 3.597958e-51 | -8.615850e-47 |
| Msa0389310 | Msa1198090 | 0.815475 | 9.071139e-52 | -8.615850e-47 |
| Msa0391050 | Msa1198090 | 0.811559 | 6.591026e-51 | -8.615850e-47 |
| Msa0411880 | Msa1198090 | 0.801952 | 7.062509e-49 | -8.615850e-47 |
| Msa0445120 | Msa1198090 | 0.816119 | 6.515995e-52 | -8.615850e-47 |
| Msa0452410 | Msa1198090 | 0.867358 | 1.451158e-65 | -8.615850e-47 |
| Msa0458700 | Msa1198090 | 0.808282 | 3.343178e-50 | -8.615850e-47 |
| Msa1002300 | Msa1198090 | 0.836292 | 1.019597e-56 | -8.615850e-47 |
| Msa1006250 | Msa1198090 | 0.830036 | 3.681394e-55 | -8.615850e-47 |
| Msa1009270 | Msa1198090 | 0.804993 | 1.654655e-49 | -8.615850e-47 |
| Msa1025790 | Msa1198090 | 0.809026 | 2.319319e-50 | -8.615850e-47 |
| Msa1027240 | Msa1198090 | 0.803637 | 3.169783e-49 | -8.615850e-47 |
| Msa1048110 | Msa1198090 | 0.800101 | 1.688017e-48 | -8.615850e-47 |
| Msa1059420 | Msa1198090 | 0.827154 | 1.830131e-54 | -8.615850e-47 |
| Msa0273570 | Msa1198090 | 0.828407 | 9.144730e-55 | -8.615850e-47 |
| Msa0273580 | Msa1198090 | 0.810611 | 1.057847e-50 | -8.615850e-47 |
| Msa0284810 | Msa1198090 | 0.816425 | 5.568267e-52 | -8.615850e-47 |
| Msa0292180 | Msa1198090 | 0.804982 | 1.662727e-49 | -8.615850e-47 |
| Msa0313780 | Msa1198090 | 0.838786 | 2.337557e-57 | -8.615850e-47 |
| Msa0313920 | Msa1198090 | 0.837009 | 6.692800e-57 | -8.615850e-47 |
| Msa0323550 | Msa1198090 | 0.814538 | 1.464385e-51 | -8.615850e-47 |
| Msa0329550 | Msa1198090 | 0.800617 | 1.325285e-48 | -8.615850e-47 |
| Msa0350670 | Msa1198090 | 0.843557 | 1.301985e-58 | -8.615850e-47 |
| Msa0352000 | Msa1198090 | 0.807854 | 4.124218e-50 | -8.615850e-47 |
| Msa0352010 | Msa1198090 | 0.818965 | 1.488009e-52 | -8.615850e-47 |
| Msa0363980 | Msa1198090 | 0.808451 | 3.077652e-50 | -8.615850e-47 |
| Msa1198090 | Msa1275770 | 0.805439 | 1.334384e-49 | -8.615850e-47 |
| Msa1198090 | Msa1288000 | 0.811759 | 5.961964e-51 | -8.615850e-47 |
| Msa1198090 | Msa1384320 | 0.818082 | 2.359849e-52 | -8.615850e-47 |
| Msa1198090 | Msa1396830 | 0.811751 | 5.986564e-51 | -8.615850e-47 |
| Msa1198090 | Msa1424690 | 0.805131 | 1.547837e-49 | -8.615850e-47 |
| Msa1198090 | Msa1444370 | 0.836650 | 8.263412e-57 | -8.615850e-47 |
| Msa0512420 | Msa1198090 | 0.818457 | 1.941101e-52 | -8.615850e-47 |
| Msa0533290 | Msa1198090 | 0.813603 | 2.354048e-51 | -8.615850e-47 |
| Msa0533430 | Msa1198090 | 0.849989 | 2.268412e-60 | -8.615850e-47 |
| Msa0580610 | Msa1198090 | 0.842548 | 2.417847e-58 | -8.615850e-47 |
| Msa0584130 | Msa1198090 | 0.844683 | 6.494950e-59 | -8.615850e-47 |
| Msa0854620 | Msa1198090 | 0.822636 | 2.130733e-53 | -8.615850e-47 |
| Msa0858360 | Msa1198090 | 0.805453 | 1.325025e-49 | -8.615850e-47 |
| Msa0863760 | Msa1198090 | 0.813852 | 2.075130e-51 | -8.615850e-47 |
| Msa0870620 | Msa1198090 | 0.832445 | 9.413110e-56 | -8.615850e-47 |
| Msa0872420 | Msa1198090 | 0.806414 | 8.320715e-50 | -8.615850e-47 |
| Msa0898350 | Msa1198090 | 0.817533 | 3.139617e-52 | -8.615850e-47 |
| Msa0898370 | Msa1198090 | 0.838125 | 3.463025e-57 | -8.615850e-47 |
| Msa0905720 | Msa1198090 | 0.820155 | 7.962498e-53 | -8.615850e-47 |
| Msa0957640 | Msa1198090 | 0.817746 | 2.810937e-52 | -8.615850e-47 |
| Msa0974910 | Msa1198090 | 0.805148 | 1.535111e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1198090 | MtrunA17_Chr8g0367301 | 82.609 | 92 | 15 | 1 | 1 | 91 | 1 | 92 | 2.29e-40 | 128 |
| Msa1198090 | MtrunA17_Chr8g0367281 | 72.043 | 93 | 24 | 2 | 1 | 91 | 1 | 93 | 2.34e-37 | 120 |
| Msa1198090 | MtrunA17_Chr8g0367291 | 72.043 | 93 | 24 | 2 | 1 | 91 | 1 | 93 | 7.14e-35 | 114 |
| Msa1198090 | MtrunA17_Chr8g0367271 | 75.000 | 76 | 18 | 1 | 1 | 75 | 1 | 76 | 3.69e-33 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCCTGGGACGTTAATTC+TGG | 0.148722 | 8_1:-49453945 | None:intergenic |
| GCCCGTGAGTGTGGGGTTCC+TGG | 0.187631 | 8_1:-49453959 | None:intergenic |
| AACTAGTTGGTCTCAATTAC+AGG | 0.309905 | 8_1:+49453882 | Msa1198090:CDS |
| CCCGTGAGTGTGGGGTTCCT+GGG | 0.423070 | 8_1:-49453958 | None:intergenic |
| GTTCCTGGGACGTTAATTCT+GGG | 0.446060 | 8_1:-49453944 | None:intergenic |
| GGATCGTGCAGACTGATACT+TGG | 0.478577 | 8_1:-49453816 | None:intergenic |
| GGGTGTCGAGCCCGTGAGTG+TGG | 0.482613 | 8_1:-49453968 | None:intergenic |
| GATCGTGCAGACTGATACTT+GGG | 0.489065 | 8_1:-49453815 | None:intergenic |
| GGTGTCGAGCCCGTGAGTGT+GGG | 0.489754 | 8_1:-49453967 | None:intergenic |
| TTAATTCTGGGACGATATTG+AGG | 0.507755 | 8_1:-49453932 | None:intergenic |
| CTGGTAACTGATGAATCCAT+TGG | 0.517670 | 8_1:-49453837 | None:intergenic |
| CCCAGGAACCCCACACTCAC+GGG | 0.521645 | 8_1:+49453958 | Msa1198090:CDS |
| ATCACTCTTACTACCGTAGC+AGG | 0.531359 | 8_1:+49453782 | Msa1198090:CDS |
| TCCCAGGAACCCCACACTCA+CGG | 0.533282 | 8_1:+49453957 | Msa1198090:CDS |
| CTGGGACGATATTGAGGTGG+CGG | 0.560311 | 8_1:-49453926 | None:intergenic |
| GGGACTAATATTTCCTGCTA+CGG | 0.564823 | 8_1:-49453795 | None:intergenic |
| TATCAGTCTGCACGATCCAA+TGG | 0.567935 | 8_1:+49453821 | Msa1198090:CDS |
| CGTCCCAGAATTAACGTCCC+AGG | 0.577444 | 8_1:+49453941 | Msa1198090:CDS |
| GGACGATATTGAGGTGGCGG+CGG | 0.578498 | 8_1:-49453923 | None:intergenic |
| GTGTCGAGCCCGTGAGTGTG+GGG | 0.595559 | 8_1:-49453966 | None:intergenic |
| ATTCTGGGACGATATTGAGG+TGG | 0.660784 | 8_1:-49453929 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTAGTTGGTCTCAATTAC+AGG | + | chr8_1:49453882-49453901 | Msa1198090:CDS | 35.0% |
| !! | TTAATTCTGGGACGATATTG+AGG | - | chr8_1:49453935-49453954 | None:intergenic | 35.0% |
| GGGACTAATATTTCCTGCTA+CGG | - | chr8_1:49453798-49453817 | None:intergenic | 40.0% | |
| CTGGTAACTGATGAATCCAT+TGG | - | chr8_1:49453840-49453859 | None:intergenic | 40.0% | |
| !! | ATTTTAGGAGGTGGGAGTAA+CGG | - | chr8_1:49453992-49454011 | None:intergenic | 40.0% |
| ATCACTCTTACTACCGTAGC+AGG | + | chr8_1:49453782-49453801 | Msa1198090:CDS | 45.0% | |
| GATCGTGCAGACTGATACTT+GGG | - | chr8_1:49453818-49453837 | None:intergenic | 45.0% | |
| TATCAGTCTGCACGATCCAA+TGG | + | chr8_1:49453821-49453840 | Msa1198090:CDS | 45.0% | |
| ! | TTTTTGTGCGTCAGCTGTTG+GGG | - | chr8_1:49453864-49453883 | None:intergenic | 45.0% |
| ! | GTTTTTGTGCGTCAGCTGTT+GGG | - | chr8_1:49453865-49453884 | None:intergenic | 45.0% |
| ! | AGTTTTTGTGCGTCAGCTGT+TGG | - | chr8_1:49453866-49453885 | None:intergenic | 45.0% |
| GCTGACGCACAAAAACTAGT+TGG | + | chr8_1:49453869-49453888 | Msa1198090:CDS | 45.0% | |
| !! | ATTCTGGGACGATATTGAGG+TGG | - | chr8_1:49453932-49453951 | None:intergenic | 45.0% |
| GTTCCTGGGACGTTAATTCT+GGG | - | chr8_1:49453947-49453966 | None:intergenic | 45.0% | |
| !! | TTTTAGGAGGTGGGAGTAAC+GGG | - | chr8_1:49453991-49454010 | None:intergenic | 45.0% |
| GGATCGTGCAGACTGATACT+TGG | - | chr8_1:49453819-49453838 | None:intergenic | 50.0% | |
| ! | TTTTGTGCGTCAGCTGTTGG+GGG | - | chr8_1:49453863-49453882 | None:intergenic | 50.0% |
| GGTTCCTGGGACGTTAATTC+TGG | - | chr8_1:49453948-49453967 | None:intergenic | 50.0% | |
| CTGGGACGATATTGAGGTGG+CGG | - | chr8_1:49453929-49453948 | None:intergenic | 55.0% | |
| CGTCCCAGAATTAACGTCCC+AGG | + | chr8_1:49453941-49453960 | Msa1198090:CDS | 55.0% | |
| ! | GGACGATATTGAGGTGGCGG+CGG | - | chr8_1:49453926-49453945 | None:intergenic | 60.0% |
| TCCCAGGAACCCCACACTCA+CGG | + | chr8_1:49453957-49453976 | Msa1198090:CDS | 60.0% | |
| CCCGTGAGTGTGGGGTTCCT+GGG | - | chr8_1:49453961-49453980 | None:intergenic | 65.0% | |
| CCCAGGAACCCCACACTCAC+GGG | + | chr8_1:49453958-49453977 | Msa1198090:CDS | 65.0% | |
| GTGTCGAGCCCGTGAGTGTG+GGG | - | chr8_1:49453969-49453988 | None:intergenic | 65.0% | |
| ! | GGTGTCGAGCCCGTGAGTGT+GGG | - | chr8_1:49453970-49453989 | None:intergenic | 65.0% |
| GTGCGTCAGCTGTTGGGGGC+TGG | - | chr8_1:49453859-49453878 | None:intergenic | 70.0% | |
| GCCCGTGAGTGTGGGGTTCC+TGG | - | chr8_1:49453962-49453981 | None:intergenic | 70.0% | |
| !! | GGGTGTCGAGCCCGTGAGTG+TGG | - | chr8_1:49453971-49453990 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 49453740 | 49454015 | 49453740 | ID=Msa1198090;Name=Msa1198090 |
| chr8_1 | mRNA | 49453740 | 49454015 | 49453740 | ID=Msa1198090-mRNA-1;Parent=Msa1198090;Name=Msa1198090-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|91 |
| chr8_1 | exon | 49453740 | 49454015 | 49453740 | ID=Msa1198090-mRNA-1:exon:15494;Parent=Msa1198090-mRNA-1 |
| chr8_1 | CDS | 49453740 | 49454015 | 49453740 | ID=Msa1198090-mRNA-1:cds;Parent=Msa1198090-mRNA-1 |
| Gene Sequence |
| Protein sequence |
