AlfalfaGEDB | Msa0518130

M. sativa cultivar zhongmu-4 / Msa0518130

KEGG: K04420
GO: GO:0000165 GO:0000186 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902533
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518130	XP_003602611.1	96.313	651	17	3	1	648	1	647	0.0	1276

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518130	sp\|F4HRJ4\|M3K3A_ARATH	54.834	662	232	17	1	648	1	609	0.0	607

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518130	G7JBK2	96.313	651	17	3	1	648	1	647	0.0	1276

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0376450	Msa0518130	0.813593	2.366583e-51	-8.615850e-47
Msa0377970	Msa0518130	0.998137	4.706321e-257	-8.615850e-47
Msa0427620	Msa0518130	0.992142	1.508919e-191	-8.615850e-47
Msa0471720	Msa0518130	0.814569	1.441246e-51	-8.615850e-47
Msa0516510	Msa0518130	0.810269	1.253556e-50	-8.615850e-47
Msa0518130	Msa0967650	0.813692	2.250218e-51	-8.615850e-47
Msa0518130	Msa1041410	0.826224	3.050658e-54	-8.615850e-47
Msa0518130	Msa1119250	0.839160	1.871079e-57	-8.615850e-47
Msa0518130	Msa1119300	0.847146	1.390734e-59	-8.615850e-47
Msa0518130	Msa1138240	0.826569	2.524554e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518130	MtrunA17_Chr3g0130151	96.313	651	17	3	1	648	1	647	0.0	1276
Msa0518130	MtrunA17_Chr8g0384301	77.778	477	97	4	175	648	231	701	0.0	710
Msa0518130	MtrunA17_Chr8g0384301	44.670	197	72	7	1	188	1	169	4.36e-20	94.7
Msa0518130	MtrunA17_Chr1g0202831	51.902	447	187	6	168	596	325	761	3.28e-145	444
Msa0518130	MtrunA17_Chr8g0386981	49.800	500	218	9	124	603	279	765	1.34e-142	437
Msa0518130	MtrunA17_Chr5g0402601	49.858	353	148	8	174	503	289	635	1.58e-108	343
Msa0518130	MtrunA17_Chr3g0133541	47.910	311	150	5	203	503	262	570	3.63e-96	310
Msa0518130	MtrunA17_Chr7g0272841	51.880	266	119	4	244	501	67	331	2.51e-88	288
Msa0518130	MtrunA17_Chr1g0205931	48.872	266	127	4	244	501	54	318	2.44e-87	286
Msa0518130	MtrunA17_Chr4g0051191	52.874	261	115	7	245	502	163	418	6.91e-81	262

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518130	AT1G53570.4	54.917	661	232	16	1	648	1	608	0.0	612
Msa0518130	AT1G53570.2	54.917	661	232	16	1	648	1	608	0.0	611
Msa0518130	AT1G53570.3	54.917	661	232	16	1	648	1	608	0.0	611
Msa0518130	AT1G53570.1	54.834	662	232	17	1	648	1	609	0.0	607
Msa0518130	AT1G53570.5	51.662	662	227	18	1	648	1	583	0.0	548
Msa0518130	AT1G53570.6	55.846	573	195	13	1	564	1	524	0.0	534
Msa0518130	AT1G53570.7	55.749	574	195	14	1	564	1	525	0.0	530
Msa0518130	AT1G63700.1	52.550	451	193	7	160	596	306	749	1.21e-144	442
Msa0518130	AT1G63700.2	52.550	451	193	7	160	596	306	749	1.21e-144	442
Msa0518130	AT5G66850.2	53.172	331	140	7	181	503	286	609	5.09e-108	341

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Find 222 sgRNAs with CRISPR-Local

Find 391 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATACCCTAAAATCTTTCT+TGG	0.318172	3_4:+69991244	Msa0518130:three_prime_UTR
AACATACTAGTTGATCCTAA+TGG	0.456682	3_4:+69988834	Msa0518130:CDS
AACCGTGGCAGGTGGAAGAG+AGG	0.616527	3_4:+69987689	Msa0518130:CDS
AACGGCAGCAGTGGCGGCGG+CGG	0.365716	3_4:+69986832	Msa0518130:CDS
AACTTGGCATTTGATCTCAT+TGG	0.407432	3_4:-69991130	None:intergenic
AAGCATCGTCGAAACTCCTT+GGG	0.481883	3_4:-69986930	None:intergenic
AAGGAGAAGAAGGAAGAAAG+AGG	0.511535	3_4:-69986688	None:intergenic
AAGGAGCACGAAGAGCCACG+TGG	0.765922	3_4:+69986793	Msa0518130:CDS
AAGTTCAACTATAGAGCACA+AGG	0.650178	3_4:-69991284	None:intergenic
AAGTTGGATGAGGTGGAGAA+AGG	0.528146	3_4:-69991063	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAAAAATCCAGTAAACCCA+AGG	+	chr3_4:69986774-69986793	Msa0518130:CDS	30.0%
CAAAGCTATAGTAATGATCA+AGG	+	chr3_4:69987099-69987118	Msa0518130:CDS	30.0%
AGGATCATCATAAGAACAAT+TGG	-	chr3_4:69987178-69987197	None:intergenic	30.0%
ACATTTGGACATGTTTACTT+AGG	+	chr3_4:69987731-69987750	Msa0518130:CDS	30.0%
ACTTAGGATTCAATAGGTAT+TGG	+	chr3_4:69987747-69987766	Msa0518130:intron	30.0%
TCAGTAATCTGTCTGTAATA+TGG	-	chr3_4:69988027-69988046	None:intergenic	30.0%
TTTAGAGGTAGAACAAAGAT+AGG	-	chr3_4:69988069-69988088	None:intergenic	30.0%
GTATTACGAATTTCCAACTT+TGG	+	chr3_4:69988095-69988114	Msa0518130:intron	30.0%
TATGTGCAATAAAAGAAGTC+AGG	+	chr3_4:69988135-69988154	Msa0518130:CDS	30.0%
ATGTGCAATAAAAGAAGTCA+GGG	+	chr3_4:69988136-69988155	Msa0518130:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	69986622	69991635	69986622	ID=Msa0518130;Name=Msa0518130
chr3_4	mRNA	69986622	69991635	69986622	ID=Msa0518130-mRNA-1;Parent=Msa0518130;Name=Msa0518130-mRNA-1;_AED=0.07;_eAED=0.07;_QI=135\|1\|1\|1\|1\|1\|11\|404\|648
chr3_4	exon	69986622	69987229	69986622	ID=Msa0518130-mRNA-1:exon:18811;Parent=Msa0518130-mRNA-1
chr3_4	exon	69987442	69987762	69987442	ID=Msa0518130-mRNA-1:exon:18812;Parent=Msa0518130-mRNA-1
chr3_4	exon	69988121	69988208	69988121	ID=Msa0518130-mRNA-1:exon:18813;Parent=Msa0518130-mRNA-1
chr3_4	exon	69988384	69988446	69988384	ID=Msa0518130-mRNA-1:exon:18814;Parent=Msa0518130-mRNA-1
chr3_4	exon	69988572	69988732	69988572	ID=Msa0518130-mRNA-1:exon:18815;Parent=Msa0518130-mRNA-1
chr3_4	exon	69988818	69988893	69988818	ID=Msa0518130-mRNA-1:exon:18816;Parent=Msa0518130-mRNA-1
chr3_4	exon	69989247	69989306	69989247	ID=Msa0518130-mRNA-1:exon:18817;Parent=Msa0518130-mRNA-1
chr3_4	exon	69989424	69989531	69989424	ID=Msa0518130-mRNA-1:exon:18818;Parent=Msa0518130-mRNA-1
chr3_4	exon	69989638	69989874	69989638	ID=Msa0518130-mRNA-1:exon:18819;Parent=Msa0518130-mRNA-1
chr3_4	exon	69990747	69990850	69990747	ID=Msa0518130-mRNA-1:exon:18820;Parent=Msa0518130-mRNA-1
chr3_4	exon	69990976	69991635	69990976	ID=Msa0518130-mRNA-1:exon:18821;Parent=Msa0518130-mRNA-1
chr3_4	five_prime_UTR	69986622	69986756	69986622	ID=Msa0518130-mRNA-1:five_prime_utr;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69986757	69987229	69986757	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69987442	69987762	69987442	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69988121	69988208	69988121	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69988384	69988446	69988384	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69988572	69988732	69988572	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69988818	69988893	69988818	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69989247	69989306	69989247	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69989424	69989531	69989424	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69989638	69989874	69989638	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69990747	69990850	69990747	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	CDS	69990976	69991231	69990976	ID=Msa0518130-mRNA-1:cds;Parent=Msa0518130-mRNA-1
chr3_4	three_prime_UTR	69991232	69991635	69991232	ID=Msa0518130-mRNA-1:three_prime_utr;Parent=Msa0518130-mRNA-1

Gene Sequence

>Msa0518130

ATGCCCACTTGGTGGGGTAAAAAATCCAGTAAACCCAAGGAGCACGAAGAGCCACGTGGCGGCAGCAGCACCAGCAACGGCAGCAGTGGCGGCGGCGGCGGTGTTCTTCAGTTCAATTTTAGCAACAAGTCTCCGATCAGAAACTCTAATAAAAACGGTTATAAACCCGAAAACCCAAGGAGTTTCGACGATGCTTCTTCTAATTTTAGAAAGTCTCCCAGATCCAGTAGAGATTTCTCTCCTTCAATCAATTCTTCCGGCGCCGCCGGCGGTGGTTCTTCCTCTTGTTGGGATTCTGATGCCGGTGATAAGATAGGACTTCCTTTGCCTCGTCCTTCTACACAAAGCTATAGTAATGATCAAGGAGGAATTACTTTTGGATTTGGATCTGCTTCTCTTTCCGGTTCCAGTGTTAGCTCCAATTGTTCTTATGATGATCCTTCAACTTCTAACTCTCAGATTAATACCGCTAGAGGACAATGTGAAACGAAGTTCAATGTCAGGCCTAAAAGCCCGGGTCCTGGTTCAAGAGGGCCTACAAGCCCAACATCACCTCTTCATCCAAGGTTGAATGTTTTGAGTCTTGACTCACCTACAGGCAGGCAAGAAGATGGAAGGAGTGAGTGTCATCCATTGCCTCTTCCACCGGGCTCTCCGACTAGTTCTTCTTCTCTTCCCAACAACACGCGACCAAATGGACTGATAGAAAGCAGTACCACTAACCGTGGCAGGTGGAAGAGAGGAAAGCTTCTAGGTCGGGGAACATTTGGACATGTTTACTTAGGATTCAATAGTGAAAGTGGACAATTATGTGCAATAAAAGAAGTCAGGGCTGTCTGTGATGATCAAACCTCTAAAGAGTGCCTCAAACAGCTTAATCAGGAGATAATTTTGCTGAGTAAACTTTCACATCCAAACATTGTTCAATACTATGGAAGTGATTTGGGAGAAGAAACACTTTCCGTTTATTTGGAATATGTCTCTGGAGGTTCTATTCATAAATTACTTCAGGAATATGGGGCCTTCAAAGAGCCTGTTATCCAAAATTATACTAGGCAGATTGTCTCTGGACTTTCCTATCTACATGCGAGAAATACAGTGCATAGGGATATTAAAGGGGCTAACATACTAGTTGATCCTAATGGTGAAATCAAGCTGGCGGACTTTGGAATGGCTAAACATATAAATTCTTCATCCTCAATGCTTTCTTTCAAAGGAAGTCCACACTGGATGGCACCAGAGGTTGTAATGAATACAAATGGCTATAGTCTTCCTGTTGATATTTGGAGCTTGGGATGCACAATTCTTGAAATGGCAACCTCAAGGCCTCCTTGGAGTCAGTATGAAGGGGTGGCTGCAATATTTAAAATCGGGAACAGCAAAGACATGCCTGAAATTCCTGATCATCTCTCAAATGATGCAAAGAATTTCATTAAGCTATGCTTGCATAGAGATCCTTCAACTCGCCCTACAGCCCAAATGTTACTAAATCACCCCTTCATTCGAGATCAGTCAGGAACAAAAGCTGCTAATGTCAGAATAACCAGGGATGCTTTTCCATACATGTGTGATGGAAGCCGGACGCCGCCAGCTTCAGATCTTCACTCCAATAGAACTTGTATTGCTTCTCAGGATGCAGATTATGCGACAAAATCAGTTCTTGCAGCTTCACGGGCAGTAAGGAGCCCAAGAGACAACACAAGAATGATCACATCTTTGCCTGTATCTCCTTGTTCAAGTCCACTGCGACAGTCTGGTCCAGCTCACAAGAGCTGTTTCCTTTCTCCACCTCATCCAACTTACGCAATGATGGGACAAAATACCCTCACTTCATATGAATCATATCCAATGAGATCAAATGCCAAGTTTACTCTTGATCCTTGGCATGAAACACATATGTACAAAGTCCACACACCTGGTGCATCCCCAAGAACGAGACTTATTTGA

Protein sequence

>Msa0518130

MPTWWGKKSSKPKEHEEPRGGSSTSNGSSGGGGGVLQFNFSNKSPIRNSNKNGYKPENPRSFDDASSNFRKSPRSSRDFSPSINSSGAAGGGSSSCWDSDAGDKIGLPLPRPSTQSYSNDQGGITFGFGSASLSGSSVSSNCSYDDPSTSNSQINTARGQCETKFNVRPKSPGPGSRGPTSPTSPLHPRLNVLSLDSPTGRQEDGRSECHPLPLPPGSPTSSSSLPNNTRPNGLIESSTTNRGRWKRGKLLGRGTFGHVYLGFNSESGQLCAIKEVRAVCDDQTSKECLKQLNQEIILLSKLSHPNIVQYYGSDLGEETLSVYLEYVSGGSIHKLLQEYGAFKEPVIQNYTRQIVSGLSYLHARNTVHRDIKGANILVDPNGEIKLADFGMAKHINSSSSMLSFKGSPHWMAPEVVMNTNGYSLPVDIWSLGCTILEMATSRPPWSQYEGVAAIFKIGNSKDMPEIPDHLSNDAKNFIKLCLHRDPSTRPTAQMLLNHPFIRDQSGTKAANVRITRDAFPYMCDGSRTPPASDLHSNRTCIASQDADYATKSVLAASRAVRSPRDNTRMITSLPVSPCSSPLRQSGPAHKSCFLSPPHPTYAMMGQNTLTSYESYPMRSNAKFTLDPWHETHMYKVHTPGASPRTRLI*