AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0531550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0531550 MtrunA17_Chr4g0004571 97.697 304 7 0 1 304 1 304 0.0 614
Msa0531550 MtrunA17_Chr2g0331771 60.738 298 113 3 1 295 140 436 3.77e-122 357
Msa0531550 MtrunA17_Chr2g0331771 59.701 67 27 0 214 280 17 83 2.39e-20 90.5
Msa0531550 MtrunA17_Chr8g0366791 36.735 294 160 7 14 288 7 293 2.47e-47 160
Msa0531550 MtrunA17_Chr5g0411411 33.333 294 170 8 14 288 7 293 7.46e-42 146
Msa0531550 MtrunA17_Chr5g0411411 29.703 202 118 9 107 292 7 200 3.39e-11 62.8
Msa0531550 MtrunA17_Chr4g0046981 26.756 299 203 5 8 292 4 300 8.26e-31 117
Msa0531550 MtrunA17_Chr8g0385571 27.798 277 191 5 14 286 225 496 5.84e-27 110
Msa0531550 MtrunA17_Chr8g0385571 29.082 196 117 8 11 194 317 502 3.59e-12 66.6
Msa0531550 MtrunA17_Chr7g0252401 26.950 282 184 5 14 286 205 473 2.29e-26 108
Msa0531550 MtrunA17_Chr3g0131501 25.436 287 205 4 5 286 192 474 2.14e-25 105
Msa0531550 MtrunA17_Chr1g0201361 29.643 280 166 8 17 292 52 304 6.45e-25 102
Msa0531550 MtrunA17_Chr4g0028531 32.824 262 159 9 17 267 23 278 1.88e-24 100
Msa0531550 MtrunA17_Chr4g0028531 29.756 205 107 8 97 284 10 194 1.26e-11 64.3
Msa0531550 MtrunA17_Chr8g0383321 30.714 280 156 11 22 292 55 305 2.66e-24 100
Msa0531550 MtrunA17_Chr8g0347451 31.731 208 127 6 101 296 37 241 7.35e-23 97.1
Msa0531550 MtrunA17_Chr8g0347451 23.659 317 210 8 5 292 40 353 2.15e-17 81.6
Msa0531550 MtrunA17_Chr3g0135861 28.053 303 188 8 14 292 33 329 1.01e-22 96.3
Msa0531550 MtrunA17_Chr8g0386871 31.273 275 168 8 18 286 322 581 3.36e-22 96.7
Msa0531550 MtrunA17_Chr8g0386871 27.168 173 119 5 22 191 416 584 2.58e-11 64.3
Msa0531550 MtrunA17_Chr6g0463351 26.950 282 183 7 31 302 82 350 4.62e-22 94.7
Msa0531550 MtrunA17_Chr5g0396801 31.683 202 123 6 97 286 20 218 5.89e-22 94.4
Msa0531550 MtrunA17_Chr5g0396801 25.081 307 198 9 14 292 36 338 2.60e-17 81.3
Msa0531550 MtrunA17_Chr7g0269701 28.231 294 182 10 22 295 25 309 6.40e-22 94.0
Msa0531550 MtrunA17_Chr7g0269701 31.250 192 125 5 110 297 22 210 1.43e-15 75.9
Msa0531550 MtrunA17_Chr1g0196921 30.682 176 114 2 126 296 30 202 1.34e-19 87.0
Msa0531550 MtrunA17_Chr1g0196921 26.070 257 169 7 31 273 29 278 3.44e-19 85.9
Msa0531550 MtrunA17_Chr8g0378951 26.054 261 176 6 4 259 15 263 3.90e-19 85.9
Msa0531550 MtrunA17_Chr4g0063561 23.711 291 213 4 8 296 115 398 9.69e-19 85.9
Msa0531550 MtrunA17_Chr3g0137941 26.471 204 137 4 104 304 117 310 1.94e-18 84.7
Msa0531550 MtrunA17_Chr3g0137941 22.414 290 218 4 8 296 114 397 1.00e-17 82.8
Msa0531550 MtrunA17_Chr1g0149111 25.000 288 205 6 8 292 131 410 2.08e-18 85.1
Msa0531550 MtrunA17_Chr1g0149111 27.861 201 132 5 104 304 134 321 2.51e-17 81.6
Msa0531550 MtrunA17_Chr1g0195101 27.599 279 174 9 32 292 35 303 2.64e-17 80.9
Msa0531550 MtrunA17_Chr1g0195101 31.892 185 118 4 123 302 32 213 5.32e-15 74.3
Msa0531550 MtrunA17_Chr6g0455381 24.172 302 201 11 25 303 21 317 6.36e-17 79.7
Msa0531550 MtrunA17_Chr6g0488081 29.798 198 117 6 104 286 13 203 9.74e-17 79.3
Msa0531550 MtrunA17_Chr6g0488081 25.078 319 191 10 1 292 9 306 1.25e-16 79.0
Msa0531550 MtrunA17_Chr5g0423111 25.267 281 178 7 32 292 37 305 1.07e-16 79.3
Msa0531550 MtrunA17_Chr5g0423111 32.065 184 116 5 123 302 34 212 1.39e-15 76.3
Msa0531550 MtrunA17_Chr5g0436571 26.821 302 164 12 31 286 23 313 1.12e-16 79.3
Msa0531550 MtrunA17_Chr7g0240341 30.978 184 121 4 15 194 133 314 1.83e-16 78.6
Msa0531550 MtrunA17_Chr7g0240341 32.192 146 94 2 145 286 65 209 7.15e-16 76.6
Msa0531550 MtrunA17_Chr5g0431531 28.889 225 143 5 71 292 2 212 5.64e-16 77.0
Msa0531550 MtrunA17_Chr5g0431531 27.413 259 169 6 17 266 40 288 1.13e-13 70.5
Msa0531550 MtrunA17_Chr8g0343381 24.092 303 195 6 31 298 48 350 7.25e-16 77.0
Msa0531550 MtrunA17_Chr3g0133111 23.151 311 195 9 22 295 9 312 3.91e-15 74.7
Msa0531550 MtrunA17_Chr4g0008061 24.161 298 198 8 11 291 10 296 1.81e-14 72.4
Msa0531550 MtrunA17_Chr8g0372531 35.196 179 84 9 124 286 28 190 2.09e-14 72.4
Msa0531550 MtrunA17_Chr8g0372531 25.532 282 185 9 31 292 29 305 1.66e-12 67.0
Msa0531550 MtrunA17_Chr5g0412401 23.175 315 194 8 22 292 9 319 5.49e-14 71.2
Msa0531550 MtrunA17_Chr7g0219891 22.559 297 198 10 25 296 25 314 5.79e-14 71.2
Msa0531550 MtrunA17_Chr3g0086811 27.010 311 193 8 14 294 26 332 6.32e-14 71.2
Msa0531550 MtrunA17_Chr2g0328891 29.927 137 92 2 150 286 214 346 1.41e-13 70.5
Msa0531550 MtrunA17_Chr4g0000261 22.667 300 205 5 6 286 56 347 1.56e-13 70.5
Msa0531550 MtrunA17_Chr8g0367641 22.654 309 196 7 22 292 9 312 1.57e-13 70.1
Msa0531550 MtrunA17_Chr8g0367641 26.829 205 117 5 113 286 5 207 2.77e-11 63.2
Msa0531550 MtrunA17_Chr7g0241521 22.945 292 201 7 16 292 17 299 3.79e-13 68.6
Msa0531550 MtrunA17_Chr2g0296061 23.636 330 198 9 4 292 8 324 5.62e-13 68.6
Msa0531550 MtrunA17_Chr4g0006851 24.335 263 179 6 36 296 118 362 9.59e-13 68.2
Msa0531550 MtrunA17_Chr4g0006851 28.659 164 104 6 125 282 104 260 9.64e-11 62.0
Msa0531550 MtrunA17_Chr8g0393031 24.797 246 165 6 51 286 63 298 1.63e-12 67.0
Msa0531550 MtrunA17_Chr3g0103681 29.825 171 109 4 126 286 31 200 1.86e-12 66.6
Msa0531550 MtrunA17_Chr3g0103681 22.222 297 190 8 14 286 13 292 8.42e-11 61.6
Msa0531550 MtrunA17_Chr2g0293621 23.488 281 189 7 31 291 44 318 2.10e-12 66.6
Msa0531550 MtrunA17_Chr5g0436041 32.258 186 116 5 15 194 140 321 2.54e-12 66.6
Msa0531550 MtrunA17_Chr5g0436041 27.811 169 110 4 101 258 21 188 2.57e-11 63.5
Msa0531550 MtrunA17_Chr8g0352031 29.927 137 92 2 150 286 217 349 3.68e-12 66.2
Msa0531550 MtrunA17_Chr4g0067231 23.197 319 187 9 24 292 15 325 8.66e-12 65.1
Msa0531550 MtrunA17_Chr3g0088301 27.673 159 106 3 133 286 219 373 9.50e-12 65.1
Msa0531550 MtrunA17_Chr1g0200171 26.299 308 182 13 6 299 515 791 1.88e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0531550 AT1G79900.1 69.831 295 80 2 1 295 1 286 7.31e-141 399
Msa0531550 AT5G46800.2 34.237 295 169 7 14 288 6 295 3.94e-43 150
Msa0531550 AT5G46800.2 28.365 208 130 7 105 297 4 207 6.57e-11 62.4
Msa0531550 AT5G46800.3 34.237 295 169 7 14 288 6 295 3.94e-43 150
Msa0531550 AT5G46800.3 28.365 208 130 7 105 297 4 207 6.57e-11 62.4
Msa0531550 AT5G46800.1 34.237 295 169 7 14 288 6 295 3.94e-43 150
Msa0531550 AT5G46800.1 28.365 208 130 7 105 297 4 207 6.57e-11 62.4
Msa0531550 AT2G33820.1 26.621 293 199 5 14 292 16 306 3.82e-35 129
Msa0531550 AT2G33820.3 26.246 301 198 7 14 292 16 314 7.75e-33 123
Msa0531550 AT1G34065.2 29.329 283 169 7 14 292 73 328 2.36e-26 106
Msa0531550 AT1G34065.1 28.421 285 169 7 14 292 80 335 2.79e-26 106
Msa0531550 AT4G11440.1 32.384 281 171 9 14 286 328 597 2.95e-25 106
Msa0531550 AT4G11440.2 32.384 281 171 9 14 286 328 597 2.95e-25 106
Msa0531550 AT2G33820.2 26.222 225 156 4 76 292 3 225 5.83e-25 100
Msa0531550 AT4G39460.2 30.034 293 172 10 16 303 58 322 7.93e-25 102
Msa0531550 AT4G39460.1 30.034 293 172 10 16 303 58 322 7.93e-25 102
Msa0531550 AT4G39460.3 30.034 293 172 10 16 303 58 322 7.93e-25 102
Msa0531550 AT5G19760.1 26.667 300 192 7 14 304 16 296 1.73e-24 100
Msa0531550 AT5G01340.1 30.640 297 179 11 17 294 18 306 2.25e-23 97.8
Msa0531550 AT5G01340.1 29.767 215 120 7 97 297 5 202 2.21e-12 67.0
Msa0531550 AT4G01100.1 32.889 225 132 8 88 297 12 232 4.54e-23 97.8
Msa0531550 AT4G01100.1 23.585 318 209 8 5 292 31 344 1.48e-18 85.1
Msa0531550 AT2G33820.4 25.214 234 158 5 14 231 16 248 1.04e-22 95.5
Msa0531550 AT4G01100.2 30.962 239 132 8 88 297 12 246 1.84e-22 96.3
Msa0531550 AT4G01100.2 22.630 327 205 8 5 287 31 353 5.55e-18 83.6
Msa0531550 AT1G25380.1 28.058 278 175 7 32 292 37 306 2.28e-22 96.3
Msa0531550 AT1G25380.2 28.058 278 175 7 32 292 37 306 2.28e-22 96.3
Msa0531550 AT4G01100.3 34.444 180 109 5 124 297 6 182 4.39e-21 91.7
Msa0531550 AT4G01100.3 24.521 261 167 7 53 287 33 289 7.77e-17 79.7
Msa0531550 AT3G20240.1 25.246 305 191 5 6 286 42 333 7.07e-21 91.7
Msa0531550 AT5G42130.1 27.719 285 176 10 32 302 134 402 8.67e-21 92.0
Msa0531550 AT2G47490.1 29.577 284 167 11 32 295 33 303 1.51e-20 90.1
Msa0531550 AT5G51050.1 25.175 286 204 6 5 286 210 489 6.49e-20 90.1
Msa0531550 AT1G14140.1 25.098 255 175 6 31 273 31 281 8.87e-20 88.2
Msa0531550 AT1G14140.1 28.302 212 140 6 94 296 1 209 2.91e-16 78.2
Msa0531550 AT4G32400.1 23.448 290 215 4 8 296 106 389 9.41e-20 89.0
Msa0531550 AT1G74240.1 28.479 309 187 8 14 295 34 335 3.09e-19 87.4
Msa0531550 AT5G61810.2 26.199 271 177 8 5 267 52 307 1.26e-18 85.1
Msa0531550 AT5G61810.2 28.796 191 119 7 11 194 152 332 3.07e-11 63.5
Msa0531550 AT5G61810.1 26.199 271 177 8 5 267 195 450 4.28e-18 84.7
Msa0531550 AT5G61810.1 28.796 191 119 7 11 194 295 475 3.27e-11 64.3
Msa0531550 AT4G03115.1 28.395 243 155 8 31 266 53 283 5.36e-18 83.2
Msa0531550 AT4G03115.1 27.000 200 133 6 103 299 29 218 3.62e-12 66.2
Msa0531550 AT4G03115.3 28.395 243 155 8 31 266 85 315 8.48e-18 82.8
Msa0531550 AT4G03115.3 27.000 200 133 6 103 299 61 250 5.68e-12 65.9
Msa0531550 AT4G26180.1 24.830 294 195 8 13 287 18 304 9.39e-18 82.8
Msa0531550 AT4G26180.1 29.744 195 121 4 104 286 15 205 7.04e-16 77.4
Msa0531550 AT4G26180.2 25.641 273 177 8 14 267 19 284 1.25e-17 81.6
Msa0531550 AT4G26180.2 29.744 195 121 4 104 286 15 205 3.61e-16 77.8
Msa0531550 AT2G37890.1 29.592 196 129 5 6 194 139 332 4.43e-17 80.9
Msa0531550 AT2G37890.1 25.166 302 191 9 14 292 43 332 1.62e-14 73.6
Msa0531550 AT2G37890.1 30.729 192 118 8 105 286 41 227 2.57e-14 72.8
Msa0531550 AT1G72820.1 23.810 315 205 7 16 297 31 343 1.10e-16 79.7
Msa0531550 AT1G72820.2 23.810 315 205 7 16 297 31 343 1.10e-16 79.7
Msa0531550 AT3G54110.1 25.556 270 175 8 14 267 13 272 1.76e-16 78.6
Msa0531550 AT3G54110.1 29.787 188 109 5 126 304 31 204 4.22e-14 72.0
Msa0531550 AT1G74240.2 27.740 292 177 8 31 295 3 287 2.25e-16 78.6
Msa0531550 AT1G14560.1 25.180 278 179 8 32 286 43 314 2.64e-16 78.6
Msa0531550 AT1G14560.1 27.684 177 110 4 124 286 41 213 4.28e-11 63.2
Msa0531550 AT1G14560.2 25.869 259 163 8 32 267 43 295 4.47e-16 77.8
Msa0531550 AT3G53940.1 30.108 186 120 5 15 194 179 360 4.81e-16 78.2
Msa0531550 AT3G53940.1 29.508 183 121 3 111 286 74 255 2.64e-14 73.2
Msa0531550 AT5G58970.1 24.342 304 197 9 2 286 3 292 8.54e-16 76.6
Msa0531550 AT4G03115.6 28.139 231 149 7 41 266 2 220 1.58e-15 75.1
Msa0531550 AT4G03115.5 28.111 217 142 6 52 266 8 212 2.14e-15 74.7
Msa0531550 AT3G55640.1 30.769 195 129 4 1 191 130 322 2.77e-15 75.5
Msa0531550 AT3G55640.1 27.322 183 125 3 111 286 39 220 1.83e-14 73.2
Msa0531550 AT3G55640.1 23.701 308 197 9 12 295 34 327 4.07e-11 63.2
Msa0531550 AT3G55640.4 30.769 195 129 4 1 191 130 322 2.77e-15 75.5
Msa0531550 AT3G55640.4 27.322 183 125 3 111 286 39 220 1.83e-14 73.2
Msa0531550 AT3G55640.4 23.701 308 197 9 12 295 34 327 4.07e-11 63.2
Msa0531550 AT5G09470.1 29.936 157 104 2 134 286 77 231 3.02e-15 75.5
Msa0531550 AT5G09470.1 24.891 229 163 5 48 273 91 313 1.60e-11 64.7
Msa0531550 AT5G66380.1 26.351 296 190 10 18 292 12 300 5.90e-15 74.3
Msa0531550 AT3G55640.2 27.322 183 125 3 111 286 39 220 7.43e-15 73.6
Msa0531550 AT3G55640.3 27.322 183 125 3 111 286 39 220 7.43e-15 73.6
Msa0531550 AT4G24570.1 24.367 316 193 8 14 292 4 310 8.89e-15 73.9
Msa0531550 AT3G53940.2 29.508 183 121 3 111 286 74 255 1.27e-14 73.2
Msa0531550 AT2G39970.1 24.242 297 180 9 31 286 23 315 4.36e-14 72.0
Msa0531550 AT5G07320.1 24.364 275 195 5 14 286 207 470 7.50e-14 72.0
Msa0531550 AT5G07320.1 29.167 168 109 4 125 292 224 381 2.87e-12 67.4
Msa0531550 AT5G07320.1 25.654 191 125 5 11 194 296 476 3.60e-12 67.0
Msa0531550 AT1G14560.3 26.667 195 120 5 111 286 26 216 1.38e-13 69.3
Msa0531550 AT1G07030.1 27.962 211 140 5 94 302 25 225 1.56e-13 70.5
Msa0531550 AT1G07030.1 26.148 283 175 9 2 266 24 290 3.56e-11 63.5
Msa0531550 AT5G13490.1 29.375 160 103 3 133 286 214 369 1.63e-13 70.9
Msa0531550 AT5G13490.2 29.375 160 103 3 133 286 214 369 1.63e-13 70.9
Msa0531550 AT5G58970.2 28.191 188 113 5 126 304 32 206 4.78e-13 68.6
Msa0531550 AT4G03115.4 27.000 200 133 6 103 299 29 218 1.56e-12 66.2
Msa0531550 AT4G03115.2 27.000 200 133 6 103 299 29 218 1.77e-12 66.2
Msa0531550 AT5G15640.2 22.939 279 183 9 14 268 18 288 2.07e-12 67.0
Msa0531550 AT5G15640.1 22.939 279 183 9 14 268 25 295 2.61e-12 67.0
Msa0531550 AT2G46320.1 29.861 144 93 3 155 290 109 252 5.38e-12 66.2
Msa0531550 AT2G30160.1 27.473 182 123 3 111 292 43 215 5.84e-12 65.9
Msa0531550 AT2G46320.4 28.767 146 96 3 153 290 8 153 8.56e-12 64.3
Msa0531550 AT2G46320.3 28.767 146 96 3 153 290 8 153 1.14e-11 64.3
Msa0531550 AT2G46320.2 28.767 146 96 3 153 290 8 153 1.14e-11 64.3
Msa0531550 AT2G46320.5 28.767 146 96 3 153 290 8 153 1.14e-11 64.3
Msa0531550 AT3G51870.1 23.792 269 184 7 36 302 120 369 2.37e-11 64.3
Msa0531550 AT2G22500.1 22.727 308 198 8 22 295 9 310 2.41e-11 63.9
Msa0531550 AT5G48970.1 24.762 210 140 4 110 304 22 228 3.40e-11 63.5
Msa0531550 AT5G48970.1 24.062 320 186 9 23 292 22 334 9.41e-11 62.4

Find 87 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACAAAAGTTGTACCTTTA+TGG 0.084408 4_1:-5726052 None:intergenic
AAAGGAATTGTCTTCTGTTT+TGG 0.197837 4_1:+5725914 Msa0531550:five_prime_UTR
TGTTAAAAGCAGAACCATTT+TGG 0.220455 4_1:-5726288 None:intergenic
GGCACTGCTGGTATAATTTC+TGG 0.229075 4_1:+5726223 Msa0531550:CDS
CTTTATGGAGATTTCAATAA+TGG 0.241743 4_1:-5726037 None:intergenic
TGTGAAACTTATGTATATAT+TGG 0.262911 4_1:+5728413 Msa0531550:three_prime_UTR
GTAAGGATAGAATGGATTTC+TGG 0.274280 4_1:+5726146 Msa0531550:exon
GAATTCTTCATGTCAAAATA+AGG 0.274507 4_1:-5725875 None:intergenic
GAATTTGTGGCTGGTGGATT+TGG 0.305163 4_1:+5726199 Msa0531550:CDS
CTTTAGACAACATTTGTCTA+AGG 0.317894 4_1:-5726312 None:intergenic
TATAATTTCTGGTTATCCTT+TGG 0.324296 4_1:+5726234 Msa0531550:CDS
CAACAAAGTGGTGGCCAAAA+TGG 0.330381 4_1:+5726274 Msa0531550:CDS
GAGTTTCTTGCAAGTAGTTC+AGG 0.334182 4_1:+5726172 Msa0531550:CDS
TTATTAGTGATAACATTGTT+TGG 0.356630 4_1:+5726081 Msa0531550:five_prime_UTR
CCTGTGTTGTGGCGCGGTTT+AGG 0.358076 4_1:+5728163 Msa0531550:CDS
CTCGTCTTCACAACATCAAA+AGG 0.383330 4_1:-5728058 None:intergenic
TTGTTCTTTGTACCGTGGTA+TGG 0.393231 4_1:+5726342 Msa0531550:CDS
GCCTAATGCAACACTCTTGT+AGG 0.397929 4_1:-5727696 None:intergenic
GAAGATGGAGATCAAATGTT+TGG 0.400293 4_1:-5728379 None:intergenic
TTTATGGAGATTTCAATAAT+GGG 0.404103 4_1:-5726036 None:intergenic
TTGGAAATCACAAAATCAAA+AGG 0.404660 4_1:+5726100 Msa0531550:five_prime_UTR
GGTGGATTTGGAGGCACTGC+TGG 0.406098 4_1:+5726211 Msa0531550:CDS
GCCAGAGCATTTGTTGTGAA+TGG 0.409963 4_1:+5728196 Msa0531550:CDS
AGACAAATGTTGTCTAAAGA+AGG 0.414361 4_1:+5726316 Msa0531550:CDS
CATTATTGAAATCTCCATAA+AGG 0.423205 4_1:+5726038 Msa0531550:five_prime_UTR
TTGGTGTCTGGAGGATTAGC+GGG 0.424572 4_1:+5728010 Msa0531550:CDS
TAAAAGTAGCTAAACACATA+TGG 0.435448 4_1:+5727831 Msa0531550:CDS
CGCGGCATATATCGAGGACT+TGG 0.435598 4_1:+5727869 Msa0531550:CDS
GAATGAGAGTAAGGATAGAA+TGG 0.446826 4_1:+5726138 Msa0531550:exon
TTGAATACTATGTTGGTGTC+TGG 0.453435 4_1:+5727998 Msa0531550:CDS
TAGTTCAGGTAGAGAATTTG+TGG 0.458521 4_1:+5726186 Msa0531550:CDS
GAGTGTTGCATTAGGCGGAA+TGG 0.463700 4_1:+5727703 Msa0531550:CDS
AGTGTTGCATTAGGCGGAAT+GGG 0.466863 4_1:+5727704 Msa0531550:CDS
ACGAAGAGTGTCAGAGAAGA+AGG 0.477304 4_1:+5728136 Msa0531550:CDS
CAATGAAAACGTTCAAATGC+AGG 0.490208 4_1:+5728264 Msa0531550:CDS
TGTTCTTTGTACCGTGGTAT+GGG 0.490836 4_1:+5726343 Msa0531550:CDS
AGGAAACAATTTAGAATGGC+AGG 0.496126 4_1:+5728284 Msa0531550:exon
CACAGTAAACTAATAGAAGA+TGG 0.499642 4_1:-5728394 None:intergenic
CCGTGGTATGGGTGCACCAT+TGG 0.501937 4_1:+5726354 Msa0531550:CDS
GGTAGAGAATTTGTGGCTGG+TGG 0.502758 4_1:+5726193 Msa0531550:CDS
AGAAAGTTTGAATACTATGT+TGG 0.504957 4_1:+5727991 Msa0531550:CDS
GTTGGTGTCTGGAGGATTAG+CGG 0.505819 4_1:+5728009 Msa0531550:CDS
AAACATATGCAGTTCTCTCA+AGG 0.505854 4_1:+5727636 Msa0531550:CDS
TCCTACAAGAGTGTTGCATT+AGG 0.508226 4_1:+5727695 Msa0531550:CDS
ACAAACCAGTCAAAAGAATC+CGG 0.509431 4_1:+5727797 Msa0531550:CDS
AAGTTTATTGCTTTCACCAG+TGG 0.509839 4_1:+5727742 Msa0531550:CDS
ACCATTCACAACAAATGCTC+TGG 0.512219 4_1:-5728197 None:intergenic
ATGCAGGAAACAATTTAGAA+TGG 0.516293 4_1:+5728280 Msa0531550:exon
ACTTATGTATATATTGGAAA+GGG 0.518993 4_1:+5728419 Msa0531550:three_prime_UTR
CCTAAACCGCGCCACAACAC+AGG 0.527341 4_1:-5728163 None:intergenic
GAACTACTTGCAAGAAACTC+TGG 0.529528 4_1:-5726169 None:intergenic
TAAACACATATGGAGAAAAG+AGG 0.540728 4_1:+5727841 Msa0531550:CDS
TTTGTGGCTGGTGGATTTGG+AGG 0.542646 4_1:+5726202 Msa0531550:CDS
TGGAAATCACAAAATCAAAA+GGG 0.545181 4_1:+5726101 Msa0531550:five_prime_UTR
GCATTAGGCGGAATGGGCAC+AGG 0.549467 4_1:+5727710 Msa0531550:CDS
TCAGGTAGAGAATTTGTGGC+TGG 0.550186 4_1:+5726190 Msa0531550:CDS
AACTTATGTATATATTGGAA+AGG 0.565350 4_1:+5728418 Msa0531550:three_prime_UTR
ATGAGGGATATACCTTCTCA+TGG 0.567380 4_1:+5727902 Msa0531550:CDS
TCATGTATATAGAAACGTAA+AGG 0.570869 4_1:+5725896 Msa0531550:five_prime_UTR
GGATCTTTAGCAGAATCAGA+TGG 0.579538 4_1:-5727668 None:intergenic
GACTTGGCATCACTATAATG+AGG 0.581438 4_1:+5727885 Msa0531550:CDS
CTAAACCGCGCCACAACACA+GGG 0.586443 4_1:-5728162 None:intergenic
AACATATGCAGTTCTCTCAA+GGG 0.587290 4_1:+5727637 Msa0531550:CDS
TCGTCTTCACAACATCAAAA+GGG 0.593439 4_1:-5728057 None:intergenic
AATGCAACACTCTTGTAGGA+TGG 0.595216 4_1:-5727692 None:intergenic
TACAAGAGTGTTGCATTAGG+CGG 0.595968 4_1:+5727698 Msa0531550:CDS
CCTTCTGCTATAAAATACAA+AGG 0.598476 4_1:+5728097 Msa0531550:CDS
AAACACATATGGAGAAAAGA+GGG 0.601908 4_1:+5727842 Msa0531550:CDS
GGATACCCTGTGTTGTGGCG+CGG 0.610153 4_1:+5728157 Msa0531550:CDS
TGGTGTCTGGAGGATTAGCG+GGG 0.610871 4_1:+5728011 Msa0531550:CDS
AATACTATGTTGGTGTCTGG+AGG 0.616080 4_1:+5728001 Msa0531550:CDS
AAGAAGGATACCCTGTGTTG+TGG 0.621789 4_1:+5728152 Msa0531550:CDS
TCTGGACATATGAATACATG+AGG 0.629048 4_1:+5727933 Msa0531550:CDS
GCAACACTCTTGTAGGATGG+AGG 0.631532 4_1:-5727689 None:intergenic
TTAGGCGGAATGGGCACAGG+AGG 0.638617 4_1:+5727713 Msa0531550:CDS
ACTTGGCATCACTATAATGA+GGG 0.646915 4_1:+5727886 Msa0531550:CDS
CCAATGGTGCACCCATACCA+CGG 0.656699 4_1:-5726354 None:intergenic
CTTAGAATCAGACAACAAAG+TGG 0.668062 4_1:+5726262 Msa0531550:CDS
TGGAGAAAAGAGGGTTTGCG+CGG 0.668379 4_1:+5727851 Msa0531550:CDS
AGAATCAGACAACAAAGTGG+TGG 0.674748 4_1:+5726265 Msa0531550:CDS
CTGATTCTAAGAGTATCCAA+AGG 0.682126 4_1:-5726250 None:intergenic
GGTTTGCGCGGCATATATCG+AGG 0.692095 4_1:+5727863 Msa0531550:CDS
CTGGACATATGAATACATGA+GGG 0.692763 4_1:+5727934 Msa0531550:CDS
TGAAAAGTAACTGATGCCAA+TGG 0.710784 4_1:-5726370 None:intergenic
GTGGCGCGGTTTAGGAACTG+CGG 0.716540 4_1:+5728171 Msa0531550:CDS
GGTGTCTGGAGGATTAGCGG+GGG 0.720060 4_1:+5728012 Msa0531550:CDS
CTGCTTATGAGATTACACTG+AGG 0.757246 4_1:+5728230 Msa0531550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTTTATATATTCTATT+TGG + chr4_1:5725998-5726017 Msa0531550:five_prime_UTR 10.0%
!! TAATTATAAATATTCTTTGC+TGG - chr4_1:5727223-5727242 None:intergenic 15.0%
!! AAATAATTAATGATTGACAA+TGG - chr4_1:5727581-5727600 None:intergenic 15.0%
!! TTTATGGAGATTTCAATAAT+GGG - chr4_1:5726039-5726058 None:intergenic 20.0%
!! TTATTAGTGATAACATTGTT+TGG + chr4_1:5726081-5726100 Msa0531550:five_prime_UTR 20.0%
!! ATGCTATGATTTATTGTTTA+TGG + chr4_1:5726472-5726491 Msa0531550:intron 20.0%
!! ATATTTGTGTAAACTCATAT+TGG + chr4_1:5726913-5726932 Msa0531550:intron 20.0%
!!! CTTTTGATTTGTTTAGATAA+AGG + chr4_1:5726942-5726961 Msa0531550:intron 20.0%
!! ATTTGTTTAGATAAAGGATT+AGG + chr4_1:5726948-5726967 Msa0531550:intron 20.0%
!! ATTAGGATTAGATTAAGATT+AGG + chr4_1:5726965-5726984 Msa0531550:intron 20.0%
!! ATATTTAATATTGCTTGAGT+TGG - chr4_1:5727016-5727035 None:intergenic 20.0%
!!! AAATTTTTAGACAATTTTCG+TGG - chr4_1:5727504-5727523 None:intergenic 20.0%
!! ACGAAAATTGTCTAAAAATT+TGG + chr4_1:5727503-5727522 Msa0531550:intron 20.0%
!!! ATTATTTTTTTGTTTTTCGG+TGG + chr4_1:5727594-5727613 Msa0531550:intron 20.0%
!! TGTGAAACTTATGTATATAT+TGG + chr4_1:5728413-5728432 Msa0531550:three_prime_UTR 20.0%
!! AACTTATGTATATATTGGAA+AGG + chr4_1:5728418-5728437 Msa0531550:three_prime_UTR 20.0%
!! ACTTATGTATATATTGGAAA+GGG + chr4_1:5728419-5728438 Msa0531550:three_prime_UTR 20.0%
! GAATTCTTCATGTCAAAATA+AGG - chr4_1:5725878-5725897 None:intergenic 25.0%
! TCATGTATATAGAAACGTAA+AGG + chr4_1:5725896-5725915 Msa0531550:five_prime_UTR 25.0%
! CTTTATGGAGATTTCAATAA+TGG - chr4_1:5726040-5726059 None:intergenic 25.0%
! CATTATTGAAATCTCCATAA+AGG + chr4_1:5726038-5726057 Msa0531550:five_prime_UTR 25.0%
! TTGGAAATCACAAAATCAAA+AGG + chr4_1:5726100-5726119 Msa0531550:five_prime_UTR 25.0%
! TGGAAATCACAAAATCAAAA+GGG + chr4_1:5726101-5726120 Msa0531550:five_prime_UTR 25.0%
!!! TATAATTTCTGGTTATCCTT+TGG + chr4_1:5726234-5726253 Msa0531550:CDS 25.0%
! ATGAGAAGAAAAGAAAAAAG+TGG + chr4_1:5726773-5726792 Msa0531550:intron 25.0%
!! TTTTGATGCATCTAATTGAA+TGG - chr4_1:5727248-5727267 None:intergenic 25.0%
! TTATCTACATATGAAGTTTG+TGG + chr4_1:5727383-5727402 Msa0531550:intron 25.0%
! TATCTACATATGAAGTTTGT+GGG + chr4_1:5727384-5727403 Msa0531550:intron 25.0%
! TATTGCAACAATCAAAGAAA+TGG + chr4_1:5727446-5727465 Msa0531550:intron 25.0%
! TAAAAGTAGCTAAACACATA+TGG + chr4_1:5727831-5727850 Msa0531550:CDS 25.0%
! AGAAAGTTTGAATACTATGT+TGG + chr4_1:5727991-5728010 Msa0531550:CDS 25.0%
! AAAGGAATTGTCTTCTGTTT+TGG + chr4_1:5725914-5725933 Msa0531550:five_prime_UTR 30.0%
! AAGGAATTGTCTTCTGTTTT+GGG + chr4_1:5725915-5725934 Msa0531550:five_prime_UTR 30.0%
!!! GTTTTGGGTTTGTCAAATTA+AGG + chr4_1:5725930-5725949 Msa0531550:five_prime_UTR 30.0%
CTACAAAAGTTGTACCTTTA+TGG - chr4_1:5726055-5726074 None:intergenic 30.0%
!!! CAAAAGGGTGATTTTTTTTG+AGG + chr4_1:5726116-5726135 Msa0531550:five_prime_UTR 30.0%
!!! TTTTTTGAGGAATGAGAGTA+AGG + chr4_1:5726129-5726148 Msa0531550:five_prime_UTR 30.0%
! TGTTAAAAGCAGAACCATTT+TGG - chr4_1:5726291-5726310 None:intergenic 30.0%
CTTTAGACAACATTTGTCTA+AGG - chr4_1:5726315-5726334 None:intergenic 30.0%
AGACAAATGTTGTCTAAAGA+AGG + chr4_1:5726316-5726335 Msa0531550:CDS 30.0%
ATCAGGCATAAATCATCAAT+TGG + chr4_1:5726514-5726533 Msa0531550:intron 30.0%
TCAGGCATAAATCATCAATT+GGG + chr4_1:5726515-5726534 Msa0531550:intron 30.0%
ATCATCACAAATTGCAGTAT+AGG + chr4_1:5726586-5726605 Msa0531550:intron 30.0%
AGTATAGGATTCATGTGAAT+AGG + chr4_1:5726601-5726620 Msa0531550:intron 30.0%
AAAAAAGTGGCTGATATGAT+AGG + chr4_1:5726786-5726805 Msa0531550:intron 30.0%
! ATAGGAATCGTTTGTGATTT+TGG + chr4_1:5726804-5726823 Msa0531550:intron 30.0%
! TAGGAATCGTTTGTGATTTT+GGG + chr4_1:5726805-5726824 Msa0531550:intron 30.0%
!! ATATTGCTTGAGTTGGAAAA+AGG - chr4_1:5727009-5727028 None:intergenic 30.0%
! TAAATATTTTCACATGAGCC+AGG + chr4_1:5727029-5727048 Msa0531550:intron 30.0%
! AACAGTGCTTTTGTATATTC+TGG + chr4_1:5727305-5727324 Msa0531550:intron 30.0%
! ACAGTGCTTTTGTATATTCT+GGG + chr4_1:5727306-5727325 Msa0531550:intron 30.0%
TCTCATATAATTGATCTCTG+TGG - chr4_1:5727538-5727557 None:intergenic 30.0%
!!! ATGTGTTTAGCTACTTTTAC+AGG - chr4_1:5727830-5727849 None:intergenic 30.0%
TAAACACATATGGAGAAAAG+AGG + chr4_1:5727841-5727860 Msa0531550:CDS 30.0%
AAACACATATGGAGAAAAGA+GGG + chr4_1:5727842-5727861 Msa0531550:CDS 30.0%
! CCTTTGTATTTTATAGCAGA+AGG - chr4_1:5728100-5728119 None:intergenic 30.0%
CCTTCTGCTATAAAATACAA+AGG + chr4_1:5728097-5728116 Msa0531550:CDS 30.0%
! GAACGTTTTCATTGTTAAAC+AGG - chr4_1:5728258-5728277 None:intergenic 30.0%
ATGCAGGAAACAATTTAGAA+TGG + chr4_1:5728280-5728299 Msa0531550:exon 30.0%
CACAGTAAACTAATAGAAGA+TGG - chr4_1:5728397-5728416 None:intergenic 30.0%
GAATGAGAGTAAGGATAGAA+TGG + chr4_1:5726138-5726157 Msa0531550:exon 35.0%
! GTAAGGATAGAATGGATTTC+TGG + chr4_1:5726146-5726165 Msa0531550:exon 35.0%
TAGTTCAGGTAGAGAATTTG+TGG + chr4_1:5726186-5726205 Msa0531550:CDS 35.0%
!! CTGATTCTAAGAGTATCCAA+AGG - chr4_1:5726253-5726272 None:intergenic 35.0%
CTTAGAATCAGACAACAAAG+TGG + chr4_1:5726262-5726281 Msa0531550:CDS 35.0%
TGAAAAGTAACTGATGCCAA+TGG - chr4_1:5726373-5726392 None:intergenic 35.0%
!!! ATTGGCATCAGTTACTTTTC+AGG + chr4_1:5726372-5726391 Msa0531550:CDS 35.0%
AAAACATCCTTTGCCATCTT+TGG + chr4_1:5726701-5726720 Msa0531550:intron 35.0%
GAACATCAAGAATCCAAAGA+TGG - chr4_1:5726717-5726736 None:intergenic 35.0%
GATGTTCTTTGAATCTCTGA+TGG + chr4_1:5726730-5726749 Msa0531550:intron 35.0%
! AGGAATCGTTTGTGATTTTG+GGG + chr4_1:5726806-5726825 Msa0531550:intron 35.0%
ACTGTATCCATACCATAGTA+GGG - chr4_1:5727072-5727091 None:intergenic 35.0%
TACTGTATCCATACCATAGT+AGG - chr4_1:5727073-5727092 None:intergenic 35.0%
AAAACTGTACGACTGTTACA+TGG + chr4_1:5727108-5727127 Msa0531550:intron 35.0%
AAACTGTACGACTGTTACAT+GGG + chr4_1:5727109-5727128 Msa0531550:intron 35.0%
! ATGTCATTGTATTGGTAGGT+GGG - chr4_1:5727284-5727303 None:intergenic 35.0%
! GTTCATGTCATTGTATTGGT+AGG - chr4_1:5727288-5727307 None:intergenic 35.0%
CACTGTTCATGTCATTGTAT+TGG - chr4_1:5727292-5727311 None:intergenic 35.0%
! GCTTTTGTATATTCTGGGTA+TGG + chr4_1:5727311-5727330 Msa0531550:intron 35.0%
! TATAGCCATAAGGCATTCAA+AGG + chr4_1:5727422-5727441 Msa0531550:intron 35.0%
AATAGCCTTTGAATGCCTTA+TGG - chr4_1:5727430-5727449 None:intergenic 35.0%
AAACATATGCAGTTCTCTCA+AGG + chr4_1:5727636-5727655 Msa0531550:CDS 35.0%
AACATATGCAGTTCTCTCAA+GGG + chr4_1:5727637-5727656 Msa0531550:CDS 35.0%
AAGTTTATTGCTTTCACCAG+TGG + chr4_1:5727742-5727761 Msa0531550:CDS 35.0%
ACAAACCAGTCAAAAGAATC+CGG + chr4_1:5727797-5727816 Msa0531550:CDS 35.0%
! TACTTTTACAGGAGTTGTTC+CGG - chr4_1:5727819-5727838 None:intergenic 35.0%
ACTTGGCATCACTATAATGA+GGG + chr4_1:5727886-5727905 Msa0531550:CDS 35.0%
CAGAAGTAAAAACCATGAGA+AGG - chr4_1:5727917-5727936 None:intergenic 35.0%
! CTTCTCATGGTTTTTACTTC+TGG + chr4_1:5727915-5727934 Msa0531550:CDS 35.0%
TCTGGACATATGAATACATG+AGG + chr4_1:5727933-5727952 Msa0531550:CDS 35.0%
CTGGACATATGAATACATGA+GGG + chr4_1:5727934-5727953 Msa0531550:CDS 35.0%
! TTGAATACTATGTTGGTGTC+TGG + chr4_1:5727998-5728017 Msa0531550:CDS 35.0%
TCGTCTTCACAACATCAAAA+GGG - chr4_1:5728060-5728079 None:intergenic 35.0%
CAATGAAAACGTTCAAATGC+AGG + chr4_1:5728264-5728283 Msa0531550:CDS 35.0%
! AGGAAACAATTTAGAATGGC+AGG + chr4_1:5728284-5728303 Msa0531550:exon 35.0%
GAAGATGGAGATCAAATGTT+TGG - chr4_1:5728382-5728401 None:intergenic 35.0%
GAACTACTTGCAAGAAACTC+TGG - chr4_1:5726172-5726191 None:intergenic 40.0%
GAGTTTCTTGCAAGTAGTTC+AGG + chr4_1:5726172-5726191 Msa0531550:CDS 40.0%
AGAATCAGACAACAAAGTGG+TGG + chr4_1:5726265-5726284 Msa0531550:CDS 40.0%
! GGTTTTTGTTCTTTGTACCG+TGG + chr4_1:5726337-5726356 Msa0531550:CDS 40.0%
TTGTTCTTTGTACCGTGGTA+TGG + chr4_1:5726342-5726361 Msa0531550:CDS 40.0%
TGTTCTTTGTACCGTGGTAT+GGG + chr4_1:5726343-5726362 Msa0531550:CDS 40.0%
! GTTGATAAGATCCTTCGATC+AGG + chr4_1:5726497-5726516 Msa0531550:intron 40.0%
GATGATTTATGCCTGATCGA+AGG - chr4_1:5726511-5726530 None:intergenic 40.0%
TCAACATGATGTTGCATGTG+TGG + chr4_1:5726668-5726687 Msa0531550:intron 40.0%
CAAGAATCCAAAGATGGCAA+AGG - chr4_1:5726711-5726730 None:intergenic 40.0%
! AATCTCTGATGGATGCGATT+TGG + chr4_1:5726741-5726760 Msa0531550:intron 40.0%
CTGTATCCATACCATAGTAG+GGG - chr4_1:5727071-5727090 None:intergenic 40.0%
GAATCACATTCACATCACAC+AGG - chr4_1:5727135-5727154 None:intergenic 40.0%
! CATGTCATTGTATTGGTAGG+TGG - chr4_1:5727285-5727304 None:intergenic 40.0%
TATGGCTATACATAACCCAG+TGG - chr4_1:5727412-5727431 None:intergenic 40.0%
CTGGGTTATGTATAGCCATA+AGG + chr4_1:5727412-5727431 Msa0531550:intron 40.0%
GGATCTTTAGCAGAATCAGA+TGG - chr4_1:5727671-5727690 None:intergenic 40.0%
AATGCAACACTCTTGTAGGA+TGG - chr4_1:5727695-5727714 None:intergenic 40.0%
!! TCCTACAAGAGTGTTGCATT+AGG + chr4_1:5727695-5727714 Msa0531550:CDS 40.0%
!! TACAAGAGTGTTGCATTAGG+CGG + chr4_1:5727698-5727717 Msa0531550:CDS 40.0%
!! CGAATTTTGATCAACTCCAC+TGG - chr4_1:5727761-5727780 None:intergenic 40.0%
! TTGTTCCGGATTCTTTTGAC+TGG - chr4_1:5727805-5727824 None:intergenic 40.0%
GACTTGGCATCACTATAATG+AGG + chr4_1:5727885-5727904 Msa0531550:CDS 40.0%
ATGAGGGATATACCTTCTCA+TGG + chr4_1:5727902-5727921 Msa0531550:CDS 40.0%
ATGAGGGAAAAATTGCATCC+AGG + chr4_1:5727950-5727969 Msa0531550:CDS 40.0%
CATCCAGGTTGTAGAAAAAG+TGG + chr4_1:5727965-5727984 Msa0531550:CDS 40.0%
!!! TGACCACTTTTTCTACAACC+TGG - chr4_1:5727971-5727990 None:intergenic 40.0%
! AATACTATGTTGGTGTCTGG+AGG + chr4_1:5728001-5728020 Msa0531550:CDS 40.0%
CTCGTCTTCACAACATCAAA+AGG - chr4_1:5728061-5728080 None:intergenic 40.0%
ACCATTCACAACAAATGCTC+TGG - chr4_1:5728200-5728219 None:intergenic 40.0%
CTGCTTATGAGATTACACTG+AGG + chr4_1:5728230-5728249 Msa0531550:CDS 40.0%
TCAGGTAGAGAATTTGTGGC+TGG + chr4_1:5726190-5726209 Msa0531550:CDS 45.0%
!! GAATTTGTGGCTGGTGGATT+TGG + chr4_1:5726199-5726218 Msa0531550:CDS 45.0%
GGCACTGCTGGTATAATTTC+TGG + chr4_1:5726223-5726242 Msa0531550:CDS 45.0%
CAACAAAGTGGTGGCCAAAA+TGG + chr4_1:5726274-5726293 Msa0531550:CDS 45.0%
! ATTTTCACATGAGCCAGGTG+AGG + chr4_1:5727034-5727053 Msa0531550:intron 45.0%
TGGATCTGAATCCCCTACTA+TGG + chr4_1:5727057-5727076 Msa0531550:intron 45.0%
CTGAATCCCCTACTATGGTA+TGG + chr4_1:5727062-5727081 Msa0531550:intron 45.0%
GCCTAATGCAACACTCTTGT+AGG - chr4_1:5727699-5727718 None:intergenic 45.0%
AGTGTTGCATTAGGCGGAAT+GGG + chr4_1:5727704-5727723 Msa0531550:CDS 45.0%
!! ACGAAGAGTGTCAGAGAAGA+AGG + chr4_1:5728136-5728155 Msa0531550:CDS 45.0%
AAGAAGGATACCCTGTGTTG+TGG + chr4_1:5728152-5728171 Msa0531550:CDS 45.0%
!! GCCAGAGCATTTGTTGTGAA+TGG + chr4_1:5728196-5728215 Msa0531550:CDS 45.0%
!! AGATAAAAAAAAAAAATTAA+AGG + chr4_1:5725970-5725989 Msa0531550:five_prime_UTR 5.0%
!!! TAATATTAATTTTTTAGATA+TGG + chr4_1:5726625-5726644 Msa0531550:intron 5.0%
!!! TAATTATTGTTTTTTTTAAT+TGG + chr4_1:5726846-5726865 Msa0531550:intron 5.0%
!! ACAAATATAAAATTAATAAA+AGG - chr4_1:5726901-5726920 None:intergenic 5.0%
!!! TTAATTATTTTTTTGTTTTT+CGG + chr4_1:5727591-5727610 Msa0531550:intron 5.0%
GGTAGAGAATTTGTGGCTGG+TGG + chr4_1:5726193-5726212 Msa0531550:CDS 50.0%
!! TTTGTGGCTGGTGGATTTGG+AGG + chr4_1:5726202-5726221 Msa0531550:CDS 50.0%
! ATGAAGTTTGTGGGTGCCAC+TGG + chr4_1:5727393-5727412 Msa0531550:intron 50.0%
!! TGAAGTTTGTGGGTGCCACT+GGG + chr4_1:5727394-5727413 Msa0531550:intron 50.0%
! TTTTCGGTGGCAGAATGCTG+TGG + chr4_1:5727607-5727626 Msa0531550:intron 50.0%
GCAACACTCTTGTAGGATGG+AGG - chr4_1:5727692-5727711 None:intergenic 50.0%
! GAGTGTTGCATTAGGCGGAA+TGG + chr4_1:5727703-5727722 Msa0531550:CDS 50.0%
TGGAGAAAAGAGGGTTTGCG+CGG + chr4_1:5727851-5727870 Msa0531550:CDS 50.0%
! GTTGGTGTCTGGAGGATTAG+CGG + chr4_1:5728009-5728028 Msa0531550:CDS 50.0%
! TTGGTGTCTGGAGGATTAGC+GGG + chr4_1:5728010-5728029 Msa0531550:CDS 50.0%
CCAATGGTGCACCCATACCA+CGG - chr4_1:5726357-5726376 None:intergenic 55.0%
TTCACATGAGCCAGGTGAGG+TGG + chr4_1:5727037-5727056 Msa0531550:intron 55.0%
GGATTCAGATCCACCTCACC+TGG - chr4_1:5727050-5727069 None:intergenic 55.0%
GGTTTGCGCGGCATATATCG+AGG + chr4_1:5727863-5727882 Msa0531550:CDS 55.0%
CGCGGCATATATCGAGGACT+TGG + chr4_1:5727869-5727888 Msa0531550:CDS 55.0%
! TGGTGTCTGGAGGATTAGCG+GGG + chr4_1:5728011-5728030 Msa0531550:CDS 55.0%
CTAAACCGCGCCACAACACA+GGG - chr4_1:5728165-5728184 None:intergenic 55.0%
!! GGTGGATTTGGAGGCACTGC+TGG + chr4_1:5726211-5726230 Msa0531550:CDS 60.0%
CCGTGGTATGGGTGCACCAT+TGG + chr4_1:5726354-5726373 Msa0531550:CDS 60.0%
GCATTAGGCGGAATGGGCAC+AGG + chr4_1:5727710-5727729 Msa0531550:CDS 60.0%
TTAGGCGGAATGGGCACAGG+AGG + chr4_1:5727713-5727732 Msa0531550:CDS 60.0%
! GGTGTCTGGAGGATTAGCGG+GGG + chr4_1:5728012-5728031 Msa0531550:CDS 60.0%
ATTAGCGGGGGTAGCGAGCT+GGG + chr4_1:5728024-5728043 Msa0531550:CDS 60.0%
GGATACCCTGTGTTGTGGCG+CGG + chr4_1:5728157-5728176 Msa0531550:CDS 60.0%
CCTAAACCGCGCCACAACAC+AGG - chr4_1:5728166-5728185 None:intergenic 60.0%
CCTGTGTTGTGGCGCGGTTT+AGG + chr4_1:5728163-5728182 Msa0531550:CDS 60.0%
GTGGCGCGGTTTAGGAACTG+CGG + chr4_1:5728171-5728190 Msa0531550:CDS 60.0%
GATTAGCGGGGGTAGCGAGC+TGG + chr4_1:5728023-5728042 Msa0531550:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 5725864 5728449 5725864 ID=Msa0531550;Name=Msa0531550
chr4_1 mRNA 5725864 5728449 5725864 ID=Msa0531550-mRNA-1;Parent=Msa0531550;Name=Msa0531550-mRNA-1;_AED=0.04;_eAED=0.04;_QI=293|1|1|1|1|1|2|152|304
chr4_1 exon 5725864 5726393 5725864 ID=Msa0531550-mRNA-1:exon:1865;Parent=Msa0531550-mRNA-1
chr4_1 exon 5727620 5728449 5727620 ID=Msa0531550-mRNA-1:exon:1866;Parent=Msa0531550-mRNA-1
chr4_1 five_prime_UTR 5725864 5726156 5725864 ID=Msa0531550-mRNA-1:five_prime_utr;Parent=Msa0531550-mRNA-1
chr4_1 CDS 5726157 5726393 5726157 ID=Msa0531550-mRNA-1:cds;Parent=Msa0531550-mRNA-1
chr4_1 CDS 5727620 5728297 5727620 ID=Msa0531550-mRNA-1:cds;Parent=Msa0531550-mRNA-1
chr4_1 three_prime_UTR 5728298 5728449 5728298 ID=Msa0531550-mRNA-1:three_prime_utr;Parent=Msa0531550-mRNA-1
Gene Sequence

>Msa0531550

ATGGATTTCTGGCCAGAGTTTCTTGCAAGTAGTTCAGGTAGAGAATTTGTGGCTGGTGGATTTGGAGGCACTGCTGGTATAATTTCTGGTTATCCTTTGGATACTCTTAGAATCAGACAACAAAGTGGTGGCCAAAATGGTTCTGCTTTTAACATCCTTAGACAAATGTTGTCTAAAGAAGGTTTTTGTTCTTTGTACCGTGGTATGGGTGCACCATTGGCATCAGTTACTTTTCAGAATGCTGTGGTTTTTCAAACATATGCAGTTCTCTCAAGGGCATTTGATCCATCTGATTCTGCTAAAGATCCTCCATCCTACAAGAGTGTTGCATTAGGCGGAATGGGCACAGGAGGTCTTCAAAGTTTATTGCTTTCACCAGTGGAGTTGATCAAAATTCGACTTCAGCTGCATAACACAAACCAGTCAAAAGAATCCGGAACAACTCCTGTAAAAGTAGCTAAACACATATGGAGAAAAGAGGGTTTGCGCGGCATATATCGAGGACTTGGCATCACTATAATGAGGGATATACCTTCTCATGGTTTTTACTTCTGGACATATGAATACATGAGGGAAAAATTGCATCCAGGTTGTAGAAAAAGTGGTCAAGAAAGTTTGAATACTATGTTGGTGTCTGGAGGATTAGCGGGGGTAGCGAGCTGGGTTTGTTGCTACCCTTTTGATGTTGTGAAGACGAGACTACAAGCTCAAACGCCTTCTGCTATAAAATACAAAGGTGTCTATGATTGTATCACGAAGAGTGTCAGAGAAGAAGGATACCCTGTGTTGTGGCGCGGTTTAGGAACTGCGGTTGCCAGAGCATTTGTTGTGAATGGTGCTGTATTTGCTGCTTATGAGATTACACTGAGGTTCCTGTTTAACAATGAAAACGTTCAAATGCAGGAAACAATTTAG
Protein sequence

>Msa0531550

MDFWPEFLASSSGREFVAGGFGGTAGIISGYPLDTLRIRQQSGGQNGSAFNILRQMLSKEGFCSLYRGMGAPLASVTFQNAVVFQTYAVLSRAFDPSDSAKDPPSYKSVALGGMGTGGLQSLLLSPVELIKIRLQLHNTNQSKESGTTPVKVAKHIWRKEGLRGIYRGLGITIMRDIPSHGFYFWTYEYMREKLHPGCRKSGQESLNTMLVSGGLAGVASWVCCYPFDVVKTRLQAQTPSAIKYKGVYDCITKSVREEGYPVLWRGLGTAVARAFVVNGAVFAAYEITLRFLFNNENVQMQETI*