Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531690 | XP_024630208.1 | 83.740 | 123 | 15 | 2 | 1 | 121 | 1 | 120 | 3.23e-56 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531690 | A0A072TGB8 | 83.740 | 123 | 15 | 2 | 1 | 121 | 1 | 120 | 1.54e-56 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0048150 | Msa0531690 | 0.809594 | 1.751677e-50 | -8.615850e-47 |
| Msa0123560 | Msa0531690 | 0.827753 | 1.314516e-54 | -8.615850e-47 |
| Msa0194950 | Msa0531690 | -0.800137 | 1.659726e-48 | -8.615850e-47 |
| Msa0398670 | Msa0531690 | 0.814100 | 1.829282e-51 | -8.615850e-47 |
| Msa0272300 | Msa0531690 | 0.806248 | 9.021839e-50 | -8.615850e-47 |
| Msa0315440 | Msa0531690 | -0.810056 | 1.393229e-50 | -8.615850e-47 |
| Msa0531530 | Msa0531690 | 0.832021 | 1.198664e-55 | -8.615850e-47 |
| Msa0531690 | Msa0588260 | 0.825119 | 5.575783e-54 | -8.615850e-47 |
| Msa0531690 | Msa0666270 | 0.821895 | 3.165376e-53 | -8.615850e-47 |
| Msa0531690 | Msa1342890 | 0.801250 | 9.837870e-49 | -8.615850e-47 |
| Msa0531690 | Msa1434420 | -0.803632 | 3.177940e-49 | -8.615850e-47 |
| Msa0531690 | Msa1457620 | 0.809505 | 1.830874e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531690 | MtrunA17_Chr1g0173081 | 83.740 | 123 | 15 | 2 | 1 | 121 | 1 | 120 | 2.97e-60 | 181 |
| Msa0531690 | MtrunA17_Chr4g0004671 | 83.740 | 123 | 15 | 2 | 1 | 121 | 1 | 120 | 5.67e-59 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0531690 | AT1G15230.1 | 42.405 | 158 | 52 | 4 | 1 | 122 | 1 | 155 | 5.86e-23 | 87.8 |
Find 37 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCCTCGCCGGCCGGCTT+TGG | 0.289427 | 4_1:-5818923 | None:intergenic |
| GAGCCGGGAGCTTGTGTTGA+TGG | 0.317537 | 4_1:+5818983 | Msa0531690:CDS |
| CAGCAAACCTGGTTGTTTCT+CGG | 0.368726 | 4_1:-5818750 | None:intergenic |
| TGTTGATGGGTTGCAAGGTT+TGG | 0.372075 | 4_1:+5818997 | Msa0531690:CDS |
| TCGAAAATATTCACATCTTC+CGG | 0.378538 | 4_1:+5818878 | Msa0531690:CDS |
| AATGCTTCGTGGATTGATTC+CGG | 0.400340 | 4_1:-5818830 | None:intergenic |
| ACATAGCCACCAACCAAGAT+AGG | 0.434082 | 4_1:-5819082 | None:intergenic |
| CGGCTTTGAGAAATGCTTCG+TGG | 0.446691 | 4_1:-5818841 | None:intergenic |
| GAAAGAGAAGAAACCTATCT+TGG | 0.464765 | 4_1:+5819069 | Msa0531690:CDS |
| TTTCGATTTCACGGCGGCGG+CGG | 0.471497 | 4_1:-5818861 | None:intergenic |
| GAAAGATGATGTGAAGGAAA+AGG | 0.485530 | 4_1:+5819021 | Msa0531690:CDS |
| CGAAGATCCTGCACTTCCAC+CGG | 0.490722 | 4_1:+5818811 | Msa0531690:CDS |
| ATAATCTGCTGCAGCAAACC+TGG | 0.498161 | 4_1:-5818761 | None:intergenic |
| AGCCGGGAGCTTGTGTTGAT+GGG | 0.502950 | 4_1:+5818984 | Msa0531690:CDS |
| TGAGCCGGAAAACGAGGAGC+CGG | 0.504878 | 4_1:+5818967 | Msa0531690:CDS |
| ATTGATTCCGGTGGAAGTGC+AGG | 0.512710 | 4_1:-5818818 | None:intergenic |
| GAGCCGGAAAACGAGGAGCC+GGG | 0.513089 | 4_1:+5818968 | Msa0531690:CDS |
| GCTTGTGTTGATGGGTTGCA+AGG | 0.538167 | 4_1:+5818992 | Msa0531690:CDS |
| GAGAAGAAACCTATCTTGGT+TGG | 0.545774 | 4_1:+5819073 | Msa0531690:CDS |
| CGATCCAAAGCCGGCCGGCG+AGG | 0.558176 | 4_1:+5818919 | Msa0531690:CDS |
| GACATCTGAAGCATCCTCGC+CGG | 0.571070 | 4_1:-5818933 | None:intergenic |
| GTCGATGCAATCATCTTCGC+CGG | 0.573460 | 4_1:-5818897 | None:intergenic |
| TTTGGAGAAAGATGATGTGA+AGG | 0.575030 | 4_1:+5819015 | Msa0531690:CDS |
| GAGTCCACCGAGAAACAACC+AGG | 0.578103 | 4_1:+5818743 | Msa0531690:CDS |
| TTGCATCGACGATCCAAAGC+CGG | 0.583740 | 4_1:+5818910 | Msa0531690:CDS |
| AACCCATCAACACAAGCTCC+CGG | 0.588157 | 4_1:-5818986 | None:intergenic |
| ATCGACGATCCAAAGCCGGC+CGG | 0.592029 | 4_1:+5818914 | Msa0531690:CDS |
| TCTGAAGCATCCTCGCCGGC+CGG | 0.595069 | 4_1:-5818929 | None:intergenic |
| AAGAAACCTATCTTGGTTGG+TGG | 0.604979 | 4_1:+5819076 | Msa0531690:CDS |
| GAGGATGCTTCAGATGTCGT+TGG | 0.615781 | 4_1:+5818938 | Msa0531690:CDS |
| CAAACCTGGTTGTTTCTCGG+TGG | 0.627408 | 4_1:-5818747 | None:intergenic |
| TGTCGTTGGTGTGATTGAGC+CGG | 0.633135 | 4_1:+5818952 | Msa0531690:CDS |
| GCTTCGTGGATTGATTCCGG+TGG | 0.637305 | 4_1:-5818827 | None:intergenic |
| TGTGATTGAGCCGGAAAACG+AGG | 0.638081 | 4_1:+5818961 | Msa0531690:CDS |
| AGACCAGCGTCTTCTAGACG+CGG | 0.719055 | 4_1:-5818788 | None:intergenic |
| CCACCGCGTCTAGAAGACGC+TGG | 0.721185 | 4_1:+5818785 | Msa0531690:CDS |
| CCAGCGTCTTCTAGACGCGG+TGG | 0.725582 | 4_1:-5818785 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTGAATATTTTCGATTTCA+CGG | - | chr4_1:5818873-5818892 | None:intergenic | 25.0% |
| ! | AAAGGAAAAAAGTGAAGAAA+AGG | + | chr4_1:5819039-5819058 | Msa0531690:CDS | 25.0% |
| TCGAAAATATTCACATCTTC+CGG | + | chr4_1:5818878-5818897 | Msa0531690:CDS | 30.0% | |
| ! | GAATATTTTCGATTTCACGG+CGG | - | chr4_1:5818870-5818889 | None:intergenic | 35.0% |
| TTTGGAGAAAGATGATGTGA+AGG | + | chr4_1:5819015-5819034 | Msa0531690:CDS | 35.0% | |
| GAAAGATGATGTGAAGGAAA+AGG | + | chr4_1:5819021-5819040 | Msa0531690:CDS | 35.0% | |
| GAAAGAGAAGAAACCTATCT+TGG | + | chr4_1:5819069-5819088 | Msa0531690:CDS | 35.0% | |
| ! | AATGCTTCGTGGATTGATTC+CGG | - | chr4_1:5818833-5818852 | None:intergenic | 40.0% |
| GAGAAGAAACCTATCTTGGT+TGG | + | chr4_1:5819073-5819092 | Msa0531690:CDS | 40.0% | |
| AAGAAACCTATCTTGGTTGG+TGG | + | chr4_1:5819076-5819095 | Msa0531690:CDS | 40.0% | |
| !! | CAGCAAACCTGGTTGTTTCT+CGG | - | chr4_1:5818753-5818772 | None:intergenic | 45.0% |
| ATAATCTGCTGCAGCAAACC+TGG | - | chr4_1:5818764-5818783 | None:intergenic | 45.0% | |
| ! | TATTTTCGATTTCACGGCGG+CGG | - | chr4_1:5818867-5818886 | None:intergenic | 45.0% |
| !! | TGTTGATGGGTTGCAAGGTT+TGG | + | chr4_1:5818997-5819016 | Msa0531690:CDS | 45.0% |
| ACATAGCCACCAACCAAGAT+AGG | - | chr4_1:5819085-5819104 | None:intergenic | 45.0% | |
| !! | CAAACCTGGTTGTTTCTCGG+TGG | - | chr4_1:5818750-5818769 | None:intergenic | 50.0% |
| ! | ATTGATTCCGGTGGAAGTGC+AGG | - | chr4_1:5818821-5818840 | None:intergenic | 50.0% |
| !! | CGGCTTTGAGAAATGCTTCG+TGG | - | chr4_1:5818844-5818863 | None:intergenic | 50.0% |
| GTCGATGCAATCATCTTCGC+CGG | - | chr4_1:5818900-5818919 | None:intergenic | 50.0% | |
| ! | TTGCATCGACGATCCAAAGC+CGG | + | chr4_1:5818910-5818929 | Msa0531690:CDS | 50.0% |
| GAGGATGCTTCAGATGTCGT+TGG | + | chr4_1:5818938-5818957 | Msa0531690:CDS | 50.0% | |
| TGTCGTTGGTGTGATTGAGC+CGG | + | chr4_1:5818952-5818971 | Msa0531690:CDS | 50.0% | |
| TGTGATTGAGCCGGAAAACG+AGG | + | chr4_1:5818961-5818980 | Msa0531690:CDS | 50.0% | |
| AACCCATCAACACAAGCTCC+CGG | - | chr4_1:5818989-5819008 | None:intergenic | 50.0% | |
| !! | GCTTGTGTTGATGGGTTGCA+AGG | + | chr4_1:5818992-5819011 | Msa0531690:CDS | 50.0% |
| GAGTCCACCGAGAAACAACC+AGG | + | chr4_1:5818743-5818762 | Msa0531690:CDS | 55.0% | |
| AGACCAGCGTCTTCTAGACG+CGG | - | chr4_1:5818791-5818810 | None:intergenic | 55.0% | |
| CGAAGATCCTGCACTTCCAC+CGG | + | chr4_1:5818811-5818830 | Msa0531690:CDS | 55.0% | |
| ! | GCTTCGTGGATTGATTCCGG+TGG | - | chr4_1:5818830-5818849 | None:intergenic | 55.0% |
| ! | GACATCTGAAGCATCCTCGC+CGG | - | chr4_1:5818936-5818955 | None:intergenic | 55.0% |
| ! | AGCCGGGAGCTTGTGTTGAT+GGG | + | chr4_1:5818984-5819003 | Msa0531690:CDS | 55.0% |
| TTTCGATTTCACGGCGGCGG+CGG | - | chr4_1:5818864-5818883 | None:intergenic | 60.0% | |
| ATCGACGATCCAAAGCCGGC+CGG | + | chr4_1:5818914-5818933 | Msa0531690:CDS | 60.0% | |
| TGAGCCGGAAAACGAGGAGC+CGG | + | chr4_1:5818967-5818986 | Msa0531690:CDS | 60.0% | |
| GAGCCGGGAGCTTGTGTTGA+TGG | + | chr4_1:5818983-5819002 | Msa0531690:CDS | 60.0% | |
| CCAGCGTCTTCTAGACGCGG+TGG | - | chr4_1:5818788-5818807 | None:intergenic | 65.0% | |
| CCACCGCGTCTAGAAGACGC+TGG | + | chr4_1:5818785-5818804 | Msa0531690:CDS | 65.0% | |
| ! | TCTGAAGCATCCTCGCCGGC+CGG | - | chr4_1:5818932-5818951 | None:intergenic | 65.0% |
| GAGCCGGAAAACGAGGAGCC+GGG | + | chr4_1:5818968-5818987 | Msa0531690:CDS | 65.0% | |
| ! | GCTCCCGGCTCCTCGTTTTC+CGG | - | chr4_1:5818974-5818993 | None:intergenic | 65.0% |
| CGATCCAAAGCCGGCCGGCG+AGG | + | chr4_1:5818919-5818938 | Msa0531690:CDS | 75.0% | |
| ! | GCATCCTCGCCGGCCGGCTT+TGG | - | chr4_1:5818926-5818945 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 5818740 | 5819108 | 5818740 | ID=Msa0531690;Name=Msa0531690 |
| chr4_1 | mRNA | 5818740 | 5819108 | 5818740 | ID=Msa0531690-mRNA-1;Parent=Msa0531690;Name=Msa0531690-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|122 |
| chr4_1 | exon | 5818740 | 5819108 | 5818740 | ID=Msa0531690-mRNA-1:exon:1923;Parent=Msa0531690-mRNA-1 |
| chr4_1 | CDS | 5818740 | 5819108 | 5818740 | ID=Msa0531690-mRNA-1:cds;Parent=Msa0531690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
