AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0542450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0542450 MtrunA17_Chr8g0352871 85.806 155 14 1 1 147 1 155 7.32e-94 269
Msa0542450 MtrunA17_Chr3g0082861 74.839 155 30 1 7 152 2 156 2.61e-82 239
Msa0542450 MtrunA17_Chr7g0256551 69.427 157 39 1 1 148 1 157 6.78e-79 233
Msa0542450 MtrunA17_Chr4g0021731 96.512 86 3 0 1 86 3 88 1.74e-56 171
Msa0542450 MtrunA17_Chr3g0124861 54.717 159 53 3 9 149 22 179 9.64e-56 174
Msa0542450 MtrunA17_Chr4g0059571 58.042 143 60 0 4 146 21 163 1.08e-53 168
Msa0542450 MtrunA17_Chr3g0128691 60.305 131 48 2 10 137 19 148 1.87e-51 161
Msa0542450 MtrunA17_Chr5g0414931 55.479 146 59 2 8 148 15 159 3.44e-51 163
Msa0542450 MtrunA17_Chr3g0118211 51.678 149 72 0 4 152 18 166 4.05e-51 161
Msa0542450 MtrunA17_Chr1g0170741 59.854 137 52 2 8 141 7 143 5.05e-50 159
Msa0542450 MtrunA17_Chr4g0030271 29.545 132 84 2 7 138 87 209 3.61e-15 71.6
Msa0542450 MtrunA17_Chr8g0345901 29.008 131 84 1 11 141 38 159 3.66e-15 71.6
Msa0542450 MtrunA17_Chr8g0376881 30.769 143 87 3 4 146 24 154 6.90e-15 70.9
Msa0542450 MtrunA17_Chr1g0181811 28.125 128 83 2 11 138 87 205 2.58e-13 66.2
Msa0542450 MtrunA17_Chr7g0275891 31.429 140 83 4 11 150 11 137 3.60e-13 65.5
Msa0542450 MtrunA17_Chr1g0149841 29.286 140 85 3 11 150 11 136 1.18e-12 64.3
Msa0542450 MtrunA17_Chr3g0123131 28.676 136 85 2 11 146 19 142 1.21e-12 64.3
Msa0542450 MtrunA17_Chr1g0161431 30.216 139 85 3 9 147 15 141 1.44e-12 63.9
Msa0542450 MtrunA17_Chr1g0149851 31.538 130 77 3 11 140 11 128 3.11e-12 63.2
Msa0542450 MtrunA17_Chr7g0275401 30.303 132 80 3 9 140 9 128 3.28e-12 62.4
Msa0542450 MtrunA17_Chr7g0276361 26.515 132 88 1 7 138 12 134 1.59e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0542450 AT3G57040.2 67.742 155 40 1 1 145 1 155 1.23e-73 220
Msa0542450 AT3G57040.1 67.742 155 40 1 1 145 1 155 1.23e-73 220
Msa0542450 AT2G41310.1 63.871 155 48 1 1 147 1 155 1.01e-70 213
Msa0542450 AT1G59940.1 61.832 131 50 0 8 138 32 162 1.45e-55 174
Msa0542450 AT1G59940.2 61.832 131 50 0 8 138 124 254 5.10e-54 173
Msa0542450 AT3G48100.1 58.273 139 57 1 2 139 18 156 6.34e-54 168
Msa0542450 AT1G10470.3 60.305 131 52 0 8 138 19 149 7.62e-54 170
Msa0542450 AT1G10470.1 60.305 131 52 0 8 138 33 163 9.57e-54 171
Msa0542450 AT5G62920.1 61.538 130 50 0 9 138 25 154 2.17e-52 164
Msa0542450 AT1G19050.1 55.147 136 61 0 8 143 23 158 5.64e-52 164
Msa0542450 AT1G74890.1 56.934 137 59 0 8 144 17 153 9.93e-52 164
Msa0542450 AT3G56380.1 53.623 138 63 1 4 140 15 152 4.96e-51 160
Msa0542450 AT3G56380.2 53.237 139 63 2 4 140 15 153 3.18e-49 155
Msa0542450 AT2G40670.1 52.518 139 57 2 8 140 28 163 4.88e-49 155
Msa0542450 AT2G40670.2 52.143 140 57 3 8 140 28 164 3.43e-47 150
Msa0542450 AT2G07440.1 65.574 61 21 0 87 147 9 69 8.31e-23 87.8
Msa0542450 AT1G10470.2 58.491 53 22 0 86 138 35 87 3.39e-16 72.0
Msa0542450 AT2G25180.1 32.308 130 76 3 11 140 19 136 8.84e-14 67.8
Msa0542450 AT5G07210.2 28.472 144 90 3 8 151 15 145 1.96e-13 67.0
Msa0542450 AT5G60100.7 30.469 128 80 2 11 138 66 184 6.58e-13 65.5
Msa0542450 AT5G60100.6 30.469 128 80 2 11 138 66 184 6.58e-13 65.5
Msa0542450 AT5G60100.4 30.469 128 80 2 11 138 66 184 6.58e-13 65.5
Msa0542450 AT5G60100.2 30.469 128 80 2 11 138 66 184 8.13e-13 65.1
Msa0542450 AT5G02810.1 27.344 128 84 2 11 138 80 198 9.49e-13 65.1
Msa0542450 AT1G67710.1 28.148 135 85 2 9 143 11 133 1.11e-12 64.7
Msa0542450 AT5G60100.5 30.469 128 80 2 11 138 66 184 1.50e-12 64.3
Msa0542450 AT5G60100.3 30.469 128 80 2 11 138 66 184 1.50e-12 64.3
Msa0542450 AT5G60100.1 30.469 128 80 2 11 138 66 184 1.50e-12 64.3
Msa0542450 AT3G16857.1 28.889 135 84 3 9 143 37 159 1.03e-11 62.0
Msa0542450 AT3G16857.2 28.889 135 84 3 9 143 37 159 1.04e-11 62.0
Msa0542450 AT2G46790.3 25.170 147 100 3 6 152 34 170 1.99e-11 61.2
Msa0542450 AT2G46790.1 25.170 147 100 3 6 152 34 170 2.01e-11 61.2
Msa0542450 AT1G49190.1 28.788 132 82 3 10 141 35 154 3.28e-11 60.5
Msa0542450 AT5G24470.1 27.820 133 85 2 9 140 50 172 5.42e-11 60.1

Find 25 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTATGTCTTTAAAAGATT+TGG 0.160260 4_1:+27515003 None:intergenic
CTGATTGTTGAACTGGTTTC+AGG 0.173333 4_1:+27514765 None:intergenic
AGAGACTCAGTTCCATGTTT+TGG 0.251951 4_1:-27515881 Msa0542450:CDS
AGTAAGGCTTTAAAATTTCT+TGG 0.266953 4_1:-27515596 Msa0542450:CDS
TCTCACCTGCTAATCCTTGA+TGG 0.304860 4_1:+27514953 None:intergenic
GGCTTTAAAATTTCTTGGTT+TGG 0.308229 4_1:-27515591 Msa0542450:CDS
TTTACATCTGATTGTTGAAC+TGG 0.343425 4_1:+27514758 None:intergenic
TCTTGGTTTGGTTGAGGATG+TGG 0.414547 4_1:-27515579 Msa0542450:CDS
TTTGATGAAATCAAGAGTTA+AGG 0.416978 4_1:-27514720 Msa0542450:CDS
TACAATGGACTCTGCTAGTA+AGG 0.427203 4_1:-27515612 Msa0542450:CDS
TATTGTATGCCAGGGATGAC+TGG 0.449232 4_1:-27515413 Msa0542450:CDS
AAAATTTCTTGGTTTGGTTG+AGG 0.449963 4_1:-27515585 Msa0542450:CDS
ACTCTTGATTTCATCAAATG+AGG 0.455768 4_1:+27514725 None:intergenic
ATAACAGATTATTGTATGCC+AGG 0.463952 4_1:-27515422 Msa0542450:CDS
ATGTTCCATCAAGGATTAGC+AGG 0.504757 4_1:-27514958 Msa0542450:intron
TTGATAGAATGTTGATTGAG+AGG 0.516155 4_1:-27515840 Msa0542450:CDS
GATGTGGCTTCAGAAGTTCA+TGG 0.523538 4_1:-27515563 Msa0542450:CDS
ATGTGGCTTCAGAAGTTCAT+GGG 0.534908 4_1:-27515562 Msa0542450:CDS
CTTCAGAGAATGTTCCATCA+AGG 0.539392 4_1:-27514967 Msa0542450:CDS
AGATCATAGCCAGTCATCCC+TGG 0.546401 4_1:+27515404 None:intergenic
TCTGAAGACATAATCACAAT+CGG 0.548679 4_1:+27514983 None:intergenic
TAACAGATTATTGTATGCCA+GGG 0.564090 4_1:-27515421 Msa0542450:CDS
TATCATCAACAGCCAAAACA+TGG 0.580138 4_1:+27515869 None:intergenic
TGAAATGATAGTTACTACAA+TGG 0.624009 4_1:-27515627 Msa0542450:intron
TGTGGCTTCAGAAGTTCATG+GGG 0.695893 4_1:-27515561 Msa0542450:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTCTTAATATTATGA+TGG - chr4_1:27515059-27515078 Msa0542450:intron 10.0%
!!! ATTATTTTGTTTTTCTATTT+AGG - chr4_1:27515359-27515378 Msa0542450:intron 10.0%
!!! TTTGTTTTCTTTATAATTTT+GGG + chr4_1:27515500-27515519 None:intergenic 10.0%
!!! AATTTTTCTTAATATTATGA+TGG - chr4_1:27515059-27515078 Msa0542450:intron 10.0%
!!! ATTATTTTGTTTTTCTATTT+AGG - chr4_1:27515359-27515378 Msa0542450:intron 10.0%
!!! TTTGTTTTCTTTATAATTTT+GGG + chr4_1:27515500-27515519 None:intergenic 10.0%
!!! CTTTGTTTTCTTTATAATTT+TGG + chr4_1:27515501-27515520 None:intergenic 15.0%
!!! CTTTGTTTTCTTTATAATTT+TGG + chr4_1:27515501-27515520 None:intergenic 15.0%
!!! ATATATGAACATAGTTTGTA+TGG - chr4_1:27514856-27514875 Msa0542450:intron 20.0%
!!! TCAATATACTATTTTGTTGA+AGG - chr4_1:27514879-27514898 Msa0542450:intron 20.0%
!! TTTCTTAAATAGAAGTGATT+TGG - chr4_1:27515407-27515426 Msa0542450:CDS 20.0%
!!! TAACATCAAGTTAACAATTT+TGG + chr4_1:27515530-27515549 None:intergenic 20.0%
!! TAAACAATTCTTTGATTCTA+AGG - chr4_1:27515554-27515573 Msa0542450:intron 20.0%
!!! ATATATGAACATAGTTTGTA+TGG - chr4_1:27514856-27514875 Msa0542450:intron 20.0%
!!! TCAATATACTATTTTGTTGA+AGG - chr4_1:27514879-27514898 Msa0542450:intron 20.0%
!! TTTCTTAAATAGAAGTGATT+TGG - chr4_1:27515407-27515426 Msa0542450:CDS 20.0%
!!! TAACATCAAGTTAACAATTT+TGG + chr4_1:27515530-27515549 None:intergenic 20.0%
!! TAAACAATTCTTTGATTCTA+AGG - chr4_1:27515554-27515573 Msa0542450:intron 20.0%
! ATTGTAATGATGTAATGTGA+TGG - chr4_1:27514827-27514846 Msa0542450:intron 25.0%
! TTGTAATGATGTAATGTGAT+GGG - chr4_1:27514828-27514847 Msa0542450:intron 25.0%
! TGAAATGATAGTTACTACAA+TGG - chr4_1:27514956-27514975 Msa0542450:intron 25.0%
!!! AGTAAGGCTTTAAAATTTCT+TGG - chr4_1:27514987-27515006 Msa0542450:CDS 25.0%
! TTGATTCATATTATTCATGC+AGG - chr4_1:27515118-27515137 Msa0542450:intron 25.0%
! TGAAAATGAGCACAAAATTA+TGG + chr4_1:27515309-27515328 None:intergenic 25.0%
!!! TGATTAGTGATTACTGATTT+GGG - chr4_1:27515469-27515488 Msa0542450:intron 25.0%
! TTTGATGAAATCAAGAGTTA+AGG - chr4_1:27515863-27515882 Msa0542450:CDS 25.0%
! ATTGTAATGATGTAATGTGA+TGG - chr4_1:27514827-27514846 Msa0542450:intron 25.0%
! TTGTAATGATGTAATGTGAT+GGG - chr4_1:27514828-27514847 Msa0542450:intron 25.0%
! TGAAATGATAGTTACTACAA+TGG - chr4_1:27514956-27514975 Msa0542450:intron 25.0%
!!! AGTAAGGCTTTAAAATTTCT+TGG - chr4_1:27514987-27515006 Msa0542450:CDS 25.0%
! TTGATTCATATTATTCATGC+AGG - chr4_1:27515118-27515137 Msa0542450:intron 25.0%
! TGAAAATGAGCACAAAATTA+TGG + chr4_1:27515309-27515328 None:intergenic 25.0%
!!! TGATTAGTGATTACTGATTT+GGG - chr4_1:27515469-27515488 Msa0542450:intron 25.0%
! TTTGATGAAATCAAGAGTTA+AGG - chr4_1:27515863-27515882 Msa0542450:CDS 25.0%
! TTGATAGAATGTTGATTGAG+AGG - chr4_1:27514743-27514762 Msa0542450:CDS 30.0%
! CATGAAAAGAAGAAGTTTTG+AGG + chr4_1:27514771-27514790 None:intergenic 30.0%
! CTCAAAACTTCTTCTTTTCA+TGG - chr4_1:27514769-27514788 Msa0542450:CDS 30.0%
!! GGCTTTAAAATTTCTTGGTT+TGG - chr4_1:27514992-27515011 Msa0542450:CDS 30.0%
!! AAAATTTCTTGGTTTGGTTG+AGG - chr4_1:27514998-27515017 Msa0542450:CDS 30.0%
ATAACAGATTATTGTATGCC+AGG - chr4_1:27515161-27515180 Msa0542450:intron 30.0%
TAACAGATTATTGTATGCCA+GGG - chr4_1:27515162-27515181 Msa0542450:intron 30.0%
! CTGATTAGTGATTACTGATT+TGG - chr4_1:27515468-27515487 Msa0542450:intron 30.0%
! CGGTATGTCTTTAAAAGATT+TGG + chr4_1:27515583-27515602 None:intergenic 30.0%
TCTGAAGACATAATCACAAT+CGG + chr4_1:27515603-27515622 None:intergenic 30.0%
ATGTGAAGTCTCACATATTA+TGG - chr4_1:27515741-27515760 Msa0542450:intron 30.0%
!!! TTGTATCAGATGTTTTGAAG+AGG - chr4_1:27515779-27515798 Msa0542450:intron 30.0%
!!! TGTATCAGATGTTTTGAAGA+GGG - chr4_1:27515780-27515799 Msa0542450:intron 30.0%
TTTACATCTGATTGTTGAAC+TGG + chr4_1:27515828-27515847 None:intergenic 30.0%
ACTCTTGATTTCATCAAATG+AGG + chr4_1:27515861-27515880 None:intergenic 30.0%
! TTGATAGAATGTTGATTGAG+AGG - chr4_1:27514743-27514762 Msa0542450:CDS 30.0%
! CATGAAAAGAAGAAGTTTTG+AGG + chr4_1:27514771-27514790 None:intergenic 30.0%
! CTCAAAACTTCTTCTTTTCA+TGG - chr4_1:27514769-27514788 Msa0542450:CDS 30.0%
!! GGCTTTAAAATTTCTTGGTT+TGG - chr4_1:27514992-27515011 Msa0542450:CDS 30.0%
!! AAAATTTCTTGGTTTGGTTG+AGG - chr4_1:27514998-27515017 Msa0542450:CDS 30.0%
ATAACAGATTATTGTATGCC+AGG - chr4_1:27515161-27515180 Msa0542450:intron 30.0%
TAACAGATTATTGTATGCCA+GGG - chr4_1:27515162-27515181 Msa0542450:intron 30.0%
! CTGATTAGTGATTACTGATT+TGG - chr4_1:27515468-27515487 Msa0542450:intron 30.0%
! CGGTATGTCTTTAAAAGATT+TGG + chr4_1:27515583-27515602 None:intergenic 30.0%
TCTGAAGACATAATCACAAT+CGG + chr4_1:27515603-27515622 None:intergenic 30.0%
ATGTGAAGTCTCACATATTA+TGG - chr4_1:27515741-27515760 Msa0542450:intron 30.0%
!!! TTGTATCAGATGTTTTGAAG+AGG - chr4_1:27515779-27515798 Msa0542450:intron 30.0%
!!! TGTATCAGATGTTTTGAAGA+GGG - chr4_1:27515780-27515799 Msa0542450:intron 30.0%
TTTACATCTGATTGTTGAAC+TGG + chr4_1:27515828-27515847 None:intergenic 30.0%
ACTCTTGATTTCATCAAATG+AGG + chr4_1:27515861-27515880 None:intergenic 30.0%
TATCATCAACAGCCAAAACA+TGG + chr4_1:27514717-27514736 None:intergenic 35.0%
TGATCTGCTCAGAAAAATCA+AGG - chr4_1:27515196-27515215 Msa0542450:intron 35.0%
TATCATCAACAGCCAAAACA+TGG + chr4_1:27514717-27514736 None:intergenic 35.0%
TGATCTGCTCAGAAAAATCA+AGG - chr4_1:27515196-27515215 Msa0542450:intron 35.0%
! AGAGACTCAGTTCCATGTTT+TGG - chr4_1:27514702-27514721 Msa0542450:CDS 40.0%
! TACAATGGACTCTGCTAGTA+AGG - chr4_1:27514971-27514990 Msa0542450:CDS 40.0%
ATGTGGCTTCAGAAGTTCAT+GGG - chr4_1:27515021-27515040 Msa0542450:intron 40.0%
CTGCTCAGAAAAATCAAGGT+AGG - chr4_1:27515200-27515219 Msa0542450:intron 40.0%
CTTCAGAGAATGTTCCATCA+AGG - chr4_1:27515616-27515635 Msa0542450:CDS 40.0%
! ATGTTCCATCAAGGATTAGC+AGG - chr4_1:27515625-27515644 Msa0542450:intron 40.0%
! CTGATTGTTGAACTGGTTTC+AGG + chr4_1:27515821-27515840 None:intergenic 40.0%
! AGAGACTCAGTTCCATGTTT+TGG - chr4_1:27514702-27514721 Msa0542450:CDS 40.0%
! TACAATGGACTCTGCTAGTA+AGG - chr4_1:27514971-27514990 Msa0542450:CDS 40.0%
ATGTGGCTTCAGAAGTTCAT+GGG - chr4_1:27515021-27515040 Msa0542450:intron 40.0%
CTGCTCAGAAAAATCAAGGT+AGG - chr4_1:27515200-27515219 Msa0542450:intron 40.0%
CTTCAGAGAATGTTCCATCA+AGG - chr4_1:27515616-27515635 Msa0542450:CDS 40.0%
! ATGTTCCATCAAGGATTAGC+AGG - chr4_1:27515625-27515644 Msa0542450:intron 40.0%
! CTGATTGTTGAACTGGTTTC+AGG + chr4_1:27515821-27515840 None:intergenic 40.0%
!! TCTTGGTTTGGTTGAGGATG+TGG - chr4_1:27515004-27515023 Msa0542450:CDS 45.0%
GATGTGGCTTCAGAAGTTCA+TGG - chr4_1:27515020-27515039 Msa0542450:intron 45.0%
TGTGGCTTCAGAAGTTCATG+GGG - chr4_1:27515022-27515041 Msa0542450:intron 45.0%
TATTGTATGCCAGGGATGAC+TGG - chr4_1:27515170-27515189 Msa0542450:intron 45.0%
TCTCACCTGCTAATCCTTGA+TGG + chr4_1:27515633-27515652 None:intergenic 45.0%
!! TCTTGGTTTGGTTGAGGATG+TGG - chr4_1:27515004-27515023 Msa0542450:CDS 45.0%
GATGTGGCTTCAGAAGTTCA+TGG - chr4_1:27515020-27515039 Msa0542450:intron 45.0%
TGTGGCTTCAGAAGTTCATG+GGG - chr4_1:27515022-27515041 Msa0542450:intron 45.0%
TATTGTATGCCAGGGATGAC+TGG - chr4_1:27515170-27515189 Msa0542450:intron 45.0%
TCTCACCTGCTAATCCTTGA+TGG + chr4_1:27515633-27515652 None:intergenic 45.0%
AGATCATAGCCAGTCATCCC+TGG + chr4_1:27515182-27515201 None:intergenic 50.0%
AGATCATAGCCAGTCATCCC+TGG + chr4_1:27515182-27515201 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 27514688 27515917 27514688 ID=Msa0542450;Name=Msa0542450
chr4_1 mRNA 27514688 27515917 27514688 ID=Msa0542450-mRNA-1;Parent=Msa0542450;Name=Msa0542450-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|1|1|5|0|152
chr4_1 exon 27515815 27515917 27515815 ID=Msa0542450-mRNA-1:exon:7439;Parent=Msa0542450-mRNA-1
chr4_1 exon 27515562 27515638 27515562 ID=Msa0542450-mRNA-1:exon:7438;Parent=Msa0542450-mRNA-1
chr4_1 exon 27515388 27515465 27515388 ID=Msa0542450-mRNA-1:exon:7437;Parent=Msa0542450-mRNA-1
chr4_1 exon 27514959 27515029 27514959 ID=Msa0542450-mRNA-1:exon:7436;Parent=Msa0542450-mRNA-1
chr4_1 exon 27514688 27514817 27514688 ID=Msa0542450-mRNA-1:exon:7435;Parent=Msa0542450-mRNA-1
chr4_1 CDS 27515815 27515917 27515815 ID=Msa0542450-mRNA-1:cds;Parent=Msa0542450-mRNA-1
chr4_1 CDS 27515562 27515638 27515562 ID=Msa0542450-mRNA-1:cds;Parent=Msa0542450-mRNA-1
chr4_1 CDS 27515388 27515465 27515388 ID=Msa0542450-mRNA-1:cds;Parent=Msa0542450-mRNA-1
chr4_1 CDS 27514959 27515029 27514959 ID=Msa0542450-mRNA-1:cds;Parent=Msa0542450-mRNA-1
chr4_1 CDS 27514688 27514817 27514688 ID=Msa0542450-mRNA-1:cds;Parent=Msa0542450-mRNA-1
Gene Sequence

>Msa0542450

ATGGGTATGGCTGCAGAGACTCAGTTCCATGTTTTGGCTGTTGATGATAGTATTATTGATAGAATGTTGATTGAGAGGCTCCTCAAAACTTCTTCTTTTCATGTTACTACAATGGACTCTGCTAGTAAGGCTTTAAAATTTCTTGGTTTGGTTGAGGATGTGGCTTCAGAAGTTCATGGGGATGTAGATGTAAATCTGATCATAACAGATTATTGTATGCCAGGGATGACTGGCTATGATCTGCTCAGAAAAATCAAGGAATCCAAATCTTTTAAAGACATACCGATTGTGATTATGTCTTCAGAGAATGTTCCATCAAGGATTAGCAGATGTTTTGAAGAGGGAGCTGAAGAATTCTTCCTGAAACCAGTTCAACAATCAGATGTAAACAAGCTGAAGCCTCATTTGATGAAATCAAGAGTTAAGGAAGAGCAAGAACAAACAACCCATCAACAATAA

Protein sequence

>Msa0542450

MGMAAETQFHVLAVDDSIIDRMLIERLLKTSSFHVTTMDSASKALKFLGLVEDVASEVHGDVDVNLIITDYCMPGMTGYDLLRKIKESKSFKDIPIVIMSSENVPSRISRCFEEGAEEFFLKPVQQSDVNKLKPHLMKSRVKEEQEQTTHQQ*