AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1433120


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGTTCTGCTTACATATT+TGG 0.081581 tig0027235:-15548 Msa1433120:CDS
AAGCTATAGAACTTAACTTT+TGG 0.253549 tig0027235:-15694 Msa1433120:CDS
TGCTTACATATTTGGATCAT+TGG 0.288800 tig0027235:-15540 Msa1433120:CDS
CTGGCGATTACTGTTGAAAA+AGG 0.307717 tig0027235:-15402 Msa1433120:CDS
CAGGTACGTCCAACAAACTA+TGG 0.362822 tig0027235:+15649 None:intergenic
GAAATTGACCGATTGAAACT+TGG 0.376665 tig0027235:+15492 None:intergenic
GAAACTTGGGCTTTCATAGC+TGG 0.377661 tig0027235:+15506 None:intergenic
GGTTGGAGACGACCGATGTT+AGG 0.401331 tig0027235:-15589 Msa1433120:CDS
AGCATTGCTTATCGCAATCT+TGG 0.427844 tig0027235:-15459 Msa1433120:CDS
TTTGCATCTACGCATTCATC+TGG 0.438204 tig0027235:-15421 Msa1433120:CDS
CTCCCTATGGATAGTATGGT+TGG 0.445956 tig0027235:-15606 Msa1433120:CDS
AAAATGCACCAATGGTGAAC+AGG 0.453002 tig0027235:+15630 None:intergenic
TTCGTTACTATTACTACCGC+TGG 0.461226 tig0027235:-15756 Msa1433120:CDS
GGAAATCCAACTTTGATGTA+TGG 0.463230 tig0027235:-15734 Msa1433120:CDS
TGGCTTTACCCATAGTTTGT+TGG 0.463391 tig0027235:-15658 Msa1433120:CDS
AAATTGACCGATTGAAACTT+GGG 0.469300 tig0027235:+15493 None:intergenic
ACCTTACCATACATCAAAGT+TGG 0.472428 tig0027235:+15728 None:intergenic
AATCTTGGTTTCTGTTGAGC+CGG 0.500259 tig0027235:-15444 Msa1433120:CDS
ATGAATGCGTAGATGCAAAC+CGG 0.525871 tig0027235:+15425 None:intergenic
TGGACGTACCTGTTCACCAT+TGG 0.540194 tig0027235:-15638 Msa1433120:CDS
TCTCCAACCATACTATCCAT+AGG 0.541270 tig0027235:+15603 None:intergenic
TCCAACTTTGATGTATGGTA+AGG 0.542081 tig0027235:-15729 Msa1433120:CDS
ATGAAAGCCCAAGTTTCAAT+CGG 0.552790 tig0027235:-15500 Msa1433120:CDS
CTCCAACCATACTATCCATA+GGG 0.555613 tig0027235:+15604 None:intergenic
CAACCTTCAACGGAAAATCC+TGG 0.564132 tig0027235:+15379 None:intergenic
AAGGAACTCAAAACATGGCT+CGG 0.577596 tig0027235:-15823 Msa1433120:CDS
GGCTCGGTTGATGAGTGTTA+CGG 0.588003 tig0027235:-15807 Msa1433120:CDS
AACAAGGAAGAAAGACAAGA+AGG 0.591524 tig0027235:-15842 Msa1433120:CDS
TCGTTACTATTACTACCGCT+GGG 0.595035 tig0027235:-15755 Msa1433120:CDS
AGGTACGTCCAACAAACTAT+GGG 0.597955 tig0027235:+15650 None:intergenic
CATGAAAGAACGCCTAACAT+CGG 0.616221 tig0027235:+15577 None:intergenic
ACAAGAAGGAACTCAAAACA+TGG 0.652113 tig0027235:-15828 Msa1433120:CDS
ATCAAAGTTGGATTTCCCAG+CGG 0.722200 tig0027235:+15740 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGCTATAGAACTTAACTTT+TGG - tig0027235:15539-15558 Msa1433120:CDS 25.0%
!! AGCTATAGAACTTAACTTTT+GGG - tig0027235:15540-15559 Msa1433120:CDS 25.0%
! TTATGTTCTGCTTACATATT+TGG - tig0027235:15685-15704 Msa1433120:CDS 25.0%
TGCTTACATATTTGGATCAT+TGG - tig0027235:15693-15712 Msa1433120:CDS 30.0%
AAATTGACCGATTGAAACTT+GGG + tig0027235:15743-15762 None:intergenic 30.0%
AACAAGGAAGAAAGACAAGA+AGG - tig0027235:15391-15410 Msa1433120:CDS 35.0%
ACAAGAAGGAACTCAAAACA+TGG - tig0027235:15405-15424 Msa1433120:CDS 35.0%
GGAAATCCAACTTTGATGTA+TGG - tig0027235:15499-15518 Msa1433120:CDS 35.0%
TCCAACTTTGATGTATGGTA+AGG - tig0027235:15504-15523 Msa1433120:CDS 35.0%
ACCTTACCATACATCAAAGT+TGG + tig0027235:15508-15527 None:intergenic 35.0%
! TTTTCTCCCTATGGATAGTA+TGG - tig0027235:15623-15642 Msa1433120:CDS 35.0%
ATGAAAGCCCAAGTTTCAAT+CGG - tig0027235:15733-15752 Msa1433120:CDS 35.0%
GAAATTGACCGATTGAAACT+TGG + tig0027235:15744-15763 None:intergenic 35.0%
AAGGAACTCAAAACATGGCT+CGG - tig0027235:15410-15429 Msa1433120:CDS 40.0%
TTCGTTACTATTACTACCGC+TGG - tig0027235:15477-15496 Msa1433120:CDS 40.0%
TCGTTACTATTACTACCGCT+GGG - tig0027235:15478-15497 Msa1433120:CDS 40.0%
ATCAAAGTTGGATTTCCCAG+CGG + tig0027235:15496-15515 None:intergenic 40.0%
!!! CTTTTGGGAAGTGCTTTTAG+TGG - tig0027235:15555-15574 Msa1433120:CDS 40.0%
! TGGCTTTACCCATAGTTTGT+TGG - tig0027235:15575-15594 Msa1433120:CDS 40.0%
AGGTACGTCCAACAAACTAT+GGG + tig0027235:15586-15605 None:intergenic 40.0%
AAAATGCACCAATGGTGAAC+AGG + tig0027235:15606-15625 None:intergenic 40.0%
TAGGGAGAAAAATGCACCAA+TGG + tig0027235:15614-15633 None:intergenic 40.0%
! TTGGTGCATTTTTCTCCCTA+TGG - tig0027235:15614-15633 Msa1433120:CDS 40.0%
CTCCAACCATACTATCCATA+GGG + tig0027235:15632-15651 None:intergenic 40.0%
TCTCCAACCATACTATCCAT+AGG + tig0027235:15633-15652 None:intergenic 40.0%
CATGAAAGAACGCCTAACAT+CGG + tig0027235:15659-15678 None:intergenic 40.0%
AGCATTGCTTATCGCAATCT+TGG - tig0027235:15774-15793 Msa1433120:CDS 40.0%
AATCTTGGTTTCTGTTGAGC+CGG - tig0027235:15789-15808 Msa1433120:CDS 40.0%
ATGAATGCGTAGATGCAAAC+CGG + tig0027235:15811-15830 None:intergenic 40.0%
TTTGCATCTACGCATTCATC+TGG - tig0027235:15812-15831 Msa1433120:CDS 40.0%
CTGGCGATTACTGTTGAAAA+AGG - tig0027235:15831-15850 Msa1433120:CDS 40.0%
GATTACTGTTGAAAAAGGCC+AGG - tig0027235:15836-15855 Msa1433120:CDS 40.0%
CAGGTACGTCCAACAAACTA+TGG + tig0027235:15587-15606 None:intergenic 45.0%
CTCCCTATGGATAGTATGGT+TGG - tig0027235:15627-15646 Msa1433120:CDS 45.0%
GAAACTTGGGCTTTCATAGC+TGG + tig0027235:15730-15749 None:intergenic 45.0%
!! GGCTCGGTTGATGAGTGTTA+CGG - tig0027235:15426-15445 Msa1433120:CDS 50.0%
TGGACGTACCTGTTCACCAT+TGG - tig0027235:15595-15614 Msa1433120:CDS 50.0%
GGTTGGAGACGACCGATGTT+AGG - tig0027235:15644-15663 Msa1433120:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0027235 gene 15385 15870 15385 ID=Msa1433120;Name=Msa1433120
tig0027235 mRNA 15385 15870 15385 ID=Msa1433120-mRNA-1;Parent=Msa1433120;Name=Msa1433120-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|161
tig0027235 exon 15385 15870 15385 ID=Msa1433120-mRNA-1:exon:8481;Parent=Msa1433120-mRNA-1
tig0027235 CDS 15385 15870 15385 ID=Msa1433120-mRNA-1:cds;Parent=Msa1433120-mRNA-1
Gene Sequence

>Msa1433120

ATGGTTAACAAGGAAGAAAGACAAGAAGGAACTCAAAACATGGCTCGGTTGATGAGTGTTACGGATGCTTTTATCATGACAACACTAATTTGTTCGTTACTATTACTACCGCTGGGAAATCCAACTTTGATGTATGGTAAGGTGCTGAATTCAAAAGCTATAGAACTTAACTTTTGGGAAGTGCTTTTAGTGGCTTTACCCATAGTTTGTTGGACGTACCTGTTCACCATTGGTGCATTTTTCTCCCTATGGATAGTATGGTTGGAGACGACCGATGTTAGGCGTTCTTTCATGACTATTTTATGTTCTGCTTACATATTTGGATCATTGGCACTTATTGTTCCAGCTATGAAAGCCCAAGTTTCAATCGGTCAATTTCAAGTGAGTGTAGCATTGCTTATCGCAATCTTGGTTTCTGTTGAGCCGGTTTGCATCTACGCATTCATCTGGCGATTACTGTTGAAAAAGGCCAGGATTTTCCGTTGA

Protein sequence

>Msa1433120

MVNKEERQEGTQNMARLMSVTDAFIMTTLICSLLLLPLGNPTLMYGKVLNSKAIELNFWEVLLVALPIVCWTYLFTIGAFFSLWIVWLETTDVRRSFMTILCSAYIFGSLALIVPAMKAQVSIGQFQVSVALLIAILVSVEPVCIYAFIWRLLLKKARIFR*