AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1121200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGTTCTGCTTACATATT+TGG 0.081581 7_3:+71287312 Msa1121200:CDS
AAGCTATAGAACTTAACTTT+TGG 0.253549 7_3:+71287166 Msa1121200:CDS
TGCTTACATATTTGGATCAT+TGG 0.288800 7_3:+71287320 Msa1121200:CDS
CTGGCGATTACTGTTGAAAA+AGG 0.307717 7_3:+71287458 Msa1121200:CDS
AGCATTGCTTATTGCAATCT+TGG 0.320492 7_3:+71287401 Msa1121200:CDS
CAGGTACGTCCAACAAACTA+TGG 0.362822 7_3:-71287211 None:intergenic
GAAATTGACCGATTGAAACT+TGG 0.376665 7_3:-71287368 None:intergenic
GAAACTTGGGCTTTCATAGC+TGG 0.377661 7_3:-71287354 None:intergenic
GGTTGGAGACGACCGATGTT+AGG 0.401331 7_3:+71287271 Msa1121200:CDS
CTCCCTATGGATAGTATGGT+TGG 0.445956 7_3:+71287254 Msa1121200:CDS
TTTGCATCTACGCATTCATC+TGG 0.452837 7_3:+71287439 Msa1121200:CDS
AAAATGCACCAATGGTGAAC+AGG 0.453002 7_3:-71287230 None:intergenic
GGAAATCCAACTTTGATGTA+TGG 0.463230 7_3:+71287126 Msa1121200:CDS
TGGCTTTACCCATAGTTTGT+TGG 0.463391 7_3:+71287202 Msa1121200:CDS
AAATTGACCGATTGAAACTT+GGG 0.469300 7_3:-71287367 None:intergenic
ACCTTACCATACATCAAAGT+TGG 0.472428 7_3:-71287132 None:intergenic
TCAGTTACTATTACTACCGC+TGG 0.521281 7_3:+71287104 Msa1121200:CDS
TGGACGTACCTGTTCACCAT+TGG 0.540194 7_3:+71287222 Msa1121200:CDS
TCTCCAACCATACTATCCAT+AGG 0.541270 7_3:-71287257 None:intergenic
TCCAACTTTGATGTATGGTA+AGG 0.542081 7_3:+71287131 Msa1121200:CDS
ATGAAAGCCCAAGTTTCAAT+CGG 0.552790 7_3:+71287360 Msa1121200:CDS
CTCCAACCATACTATCCATA+GGG 0.555613 7_3:-71287256 None:intergenic
GGCTCGGTTGATGAGTGTTA+AGG 0.561053 7_3:+71287053 Msa1121200:CDS
CAATCATCAACGGAAAATCC+TGG 0.564842 7_3:-71287481 None:intergenic
AAGGAACTCAAAACATGGCT+CGG 0.577596 7_3:+71287037 None:intergenic
CAGTTACTATTACTACCGCT+GGG 0.584102 7_3:+71287105 Msa1121200:CDS
AGGTACGTCCAACAAACTAT+GGG 0.597955 7_3:-71287210 None:intergenic
ATGAATGCGTAGATGCAAAG+AGG 0.637814 7_3:-71287435 None:intergenic
ATCAAAGTTGGATTTCCCAG+CGG 0.722200 7_3:-71287120 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGCTATAGAACTTAACTTT+TGG + chr7_3:71287166-71287185 Msa1121200:CDS 25.0%
!! AGCTATAGAACTTAACTTTT+GGG + chr7_3:71287167-71287186 Msa1121200:CDS 25.0%
! TTATGTTCTGCTTACATATT+TGG + chr7_3:71287312-71287331 Msa1121200:CDS 25.0%
TGCTTACATATTTGGATCAT+TGG + chr7_3:71287320-71287339 Msa1121200:CDS 30.0%
AAATTGACCGATTGAAACTT+GGG - chr7_3:71287370-71287389 None:intergenic 30.0%
GGAAATCCAACTTTGATGTA+TGG + chr7_3:71287126-71287145 Msa1121200:CDS 35.0%
TCCAACTTTGATGTATGGTA+AGG + chr7_3:71287131-71287150 Msa1121200:CDS 35.0%
ACCTTACCATACATCAAAGT+TGG - chr7_3:71287135-71287154 None:intergenic 35.0%
! TTTTCTCCCTATGGATAGTA+TGG + chr7_3:71287250-71287269 Msa1121200:CDS 35.0%
CATGAAAAAACGCCTAACAT+CGG - chr7_3:71287286-71287305 None:intergenic 35.0%
ATGAAAGCCCAAGTTTCAAT+CGG + chr7_3:71287360-71287379 Msa1121200:CDS 35.0%
GAAATTGACCGATTGAAACT+TGG - chr7_3:71287371-71287390 None:intergenic 35.0%
AGCATTGCTTATTGCAATCT+TGG + chr7_3:71287401-71287420 Msa1121200:CDS 35.0%
TCAGTTACTATTACTACCGC+TGG + chr7_3:71287104-71287123 Msa1121200:CDS 40.0%
CAGTTACTATTACTACCGCT+GGG + chr7_3:71287105-71287124 Msa1121200:CDS 40.0%
ATCAAAGTTGGATTTCCCAG+CGG - chr7_3:71287123-71287142 None:intergenic 40.0%
!!! CTTTTGGGAAGTGCTTTTAG+TGG + chr7_3:71287182-71287201 Msa1121200:CDS 40.0%
! TGGCTTTACCCATAGTTTGT+TGG + chr7_3:71287202-71287221 Msa1121200:CDS 40.0%
AGGTACGTCCAACAAACTAT+GGG - chr7_3:71287213-71287232 None:intergenic 40.0%
AAAATGCACCAATGGTGAAC+AGG - chr7_3:71287233-71287252 None:intergenic 40.0%
TAGGGAGAAAAATGCACCAA+TGG - chr7_3:71287241-71287260 None:intergenic 40.0%
! TTGGTGCATTTTTCTCCCTA+TGG + chr7_3:71287241-71287260 Msa1121200:CDS 40.0%
CTCCAACCATACTATCCATA+GGG - chr7_3:71287259-71287278 None:intergenic 40.0%
TCTCCAACCATACTATCCAT+AGG - chr7_3:71287260-71287279 None:intergenic 40.0%
ATGAATGCGTAGATGCAAAG+AGG - chr7_3:71287438-71287457 None:intergenic 40.0%
TTTGCATCTACGCATTCATC+TGG + chr7_3:71287439-71287458 Msa1121200:CDS 40.0%
CTGGCGATTACTGTTGAAAA+AGG + chr7_3:71287458-71287477 Msa1121200:CDS 40.0%
GATTACTGTTGAAAAAGGCC+AGG + chr7_3:71287463-71287482 Msa1121200:CDS 40.0%
CAGGTACGTCCAACAAACTA+TGG - chr7_3:71287214-71287233 None:intergenic 45.0%
CTCCCTATGGATAGTATGGT+TGG + chr7_3:71287254-71287273 Msa1121200:CDS 45.0%
GAAACTTGGGCTTTCATAGC+TGG - chr7_3:71287357-71287376 None:intergenic 45.0%
!! GGCTCGGTTGATGAGTGTTA+AGG + chr7_3:71287053-71287072 Msa1121200:CDS 50.0%
TGGACGTACCTGTTCACCAT+TGG + chr7_3:71287222-71287241 Msa1121200:CDS 50.0%
GGTTGGAGACGACCGATGTT+AGG + chr7_3:71287271-71287290 Msa1121200:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 71287051 71287497 71287051 ID=Msa1121200;Name=Msa1121200
chr7_3 mRNA 71287051 71287497 71287051 ID=Msa1121200-mRNA-1;Parent=Msa1121200;Name=Msa1121200-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|148
chr7_3 exon 71287051 71287497 71287051 ID=Msa1121200-mRNA-1:exon:18176;Parent=Msa1121200-mRNA-1
chr7_3 CDS 71287051 71287497 71287051 ID=Msa1121200-mRNA-1:cds;Parent=Msa1121200-mRNA-1
Gene Sequence

>Msa1121200

ATGGCTCGGTTGATGAGTGTTAAGGATGCTTTTATCATGACAACACTAATTTGTCAGTTACTATTACTACCGCTGGGAAATCCAACTTTGATGTATGGTAAGGTGCTGAATTCAAAAGCTATAGAACTTAACTTTTGGGAAGTGCTTTTAGTGGCTTTACCCATAGTTTGTTGGACGTACCTGTTCACCATTGGTGCATTTTTCTCCCTATGGATAGTATGGTTGGAGACGACCGATGTTAGGCGTTTTTTCATGACTATTTTATGTTCTGCTTACATATTTGGATCATTGGCACTTATTGTTCCAGCTATGAAAGCCCAAGTTTCAATCGGTCAATTTCAAGTGAGTGTAGCATTGCTTATTGCAATCTTGGTTTCTGTTGAGCCTCTTTGCATCTACGCATTCATCTGGCGATTACTGTTGAAAAAGGCCAGGATTTTCCGTTGA

Protein sequence

>Msa1121200

MARLMSVKDAFIMTTLICQLLLLPLGNPTLMYGKVLNSKAIELNFWEVLLVALPIVCWTYLFTIGAFFSLWIVWLETTDVRRFFMTILCSAYIFGSLALIVPAMKAQVSIGQFQVSVALLIAILVSVEPLCIYAFIWRLLLKKARIFR*