AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0614360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614360 MtrunA17_Chr7g0223121 52.778 72 31 1 1 69 28 99 5.65e-17 75.5
Msa0614360 MtrunA17_Chr7g0223101 73.333 45 12 0 25 69 54 98 1.85e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614360 AT1G65520.1 50.794 63 27 1 11 69 38 100 1.36e-11 61.6

Find 67 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTAGTTGTTGGGTATAC+TGG 0.280147 4_2:+63396191 Msa0614360:three_prime_UTR
TTGTGTGAACTTGGGTAAAT+AGG 0.317284 4_2:-63394426 None:intergenic
GGAGTGGTCGACTGTTAATT+TGG 0.322280 4_2:+63393769 Msa0614360:CDS
ATATGCAGAAAAGCAGAAAA+AGG 0.350964 4_2:+63392733 Msa0614360:intron
AGGTTCACTAATGCAATCTT+TGG 0.356627 4_2:+63396032 Msa0614360:intron
ACGACGGTTGATGGCTTTGT+AGG 0.357835 4_2:-63391958 None:intergenic
TGTGCCGAAACGACGGTTGA+TGG 0.357888 4_2:-63391967 None:intergenic
GAGACAGTGTTCATCGTTGC+TGG 0.373353 4_2:-63392023 None:intergenic
AAGGAAGATAATTTAGTTGT+TGG 0.376710 4_2:+63396182 Msa0614360:three_prime_UTR
CATCGTTGCTGGTTAGTGTT+AGG 0.390494 4_2:-63392012 None:intergenic
GTATGTTACTTCAATATTCC+AGG 0.392807 4_2:-63394450 None:intergenic
AAAGCCATCAACCGTCGTTT+CGG 0.395485 4_2:+63391963 Msa0614360:five_prime_UTR
AGGAAGATAATTTAGTTGTT+GGG 0.413978 4_2:+63396183 Msa0614360:three_prime_UTR
AAGTTCATATAAAAGTTTGC+AGG 0.415180 4_2:+63393801 Msa0614360:CDS
ATTCCAGGATTGTGTGAACT+TGG 0.431318 4_2:-63394435 None:intergenic
CGTGGCGGAAGAGAGATTCT+AGG 0.452169 4_2:-63391929 None:intergenic
CACCGTCACGCCTTCGCCGC+AGG 0.455545 4_2:+63392235 Msa0614360:CDS
AGGCATTTGGCAAACATGTA+TGG 0.456482 4_2:+63396114 Msa0614360:three_prime_UTR
GAAGGCGGCGATGGTTGGCA+TGG 0.457955 4_2:-63392213 None:intergenic
TTCACTAATGCAATCTTTGG+AGG 0.463708 4_2:+63396035 Msa0614360:three_prime_UTR
CGGCGAAGGCGTGACGGTGA+AGG 0.465129 4_2:-63392231 None:intergenic
ACGGTGAAGGCGGCGATGGT+TGG 0.471893 4_2:-63392218 None:intergenic
TGAAGAAATTTGAATAAGAA+AGG 0.490080 4_2:-63391904 None:intergenic
TTGATTGAATTGAGAAGTGT+GGG 0.494586 4_2:-63392049 None:intergenic
AATTTGCGCGGTGGTGATGA+AGG 0.498164 4_2:-63392086 None:intergenic
GTCGACTGTTAATTTGGTGC+TGG 0.499946 4_2:+63393775 Msa0614360:CDS
CTGTCATGTGCTGTTATCTG+TGG 0.500050 4_2:+63393709 Msa0614360:CDS
TGTGGGGACAGTTAGGGGAG+TGG 0.501787 4_2:+63393753 Msa0614360:CDS
TTCGATTTCCACTACAACAC+TGG 0.504323 4_2:+63392799 Msa0614360:CDS
TACCCAAGTTCACACAATCC+TGG 0.508621 4_2:+63394432 Msa0614360:CDS
AAGGCGGCGATGGTTGGCAT+GGG 0.512341 4_2:-63392212 None:intergenic
AGATATGCTTCAAGAGGGCG+TGG 0.520461 4_2:-63392774 None:intergenic
CAACGGCTACGACGTCGATC+GGG 0.521768 4_2:+63392115 Msa0614360:CDS
GCAAATTTGTGCCGAAACGA+CGG 0.525390 4_2:-63391974 None:intergenic
GTAACATACTACATGAGTTA+CGG 0.526123 4_2:+63394464 Msa0614360:exon
CCAACGGCTACGACGTCGAT+CGG 0.527240 4_2:+63392114 Msa0614360:CDS
CGTGACGGTGAAGGCGGCGA+TGG 0.531005 4_2:-63392222 None:intergenic
CATGGGATGAGAGAGAAGTT+CGG 0.543922 4_2:-63392195 None:intergenic
GTTGATGGCTTTGTAGGTGA+TGG 0.544556 4_2:-63391952 None:intergenic
AAATAAATACAGAAGTATGA+AGG 0.548610 4_2:+63396163 Msa0614360:three_prime_UTR
TTCCAGGATTGTGTGAACTT+GGG 0.555975 4_2:-63394434 None:intergenic
ATATGTCACCAGTGTTGTAG+TGG 0.564123 4_2:-63392807 None:intergenic
GAGGATGTAACCTGCGGCGA+AGG 0.566868 4_2:-63392245 None:intergenic
TTTGATTGAATTGAGAAGTG+TGG 0.568432 4_2:-63392050 None:intergenic
GGTGATGAAGGCGAGGAGGA+CGG 0.571578 4_2:-63392074 None:intergenic
TGTCATGTGCTGTTATCTGT+GGG 0.572424 4_2:+63393710 Msa0614360:CDS
CGAAGGCGTGACGGTGAAGG+CGG 0.577796 4_2:-63392228 None:intergenic
CGAAGGAGATATGCTTCAAG+AGG 0.578167 4_2:-63392780 None:intergenic
ATGTATAGTTTGTGATACAT+TGG 0.598181 4_2:+63396345 Msa0614360:three_prime_UTR
CCGATCGACGTCGTAGCCGT+TGG 0.609478 4_2:-63392114 None:intergenic
GCGCGGTGGTGATGAAGGCG+AGG 0.618315 4_2:-63392081 None:intergenic
CATTTGGCAAACATGTATGG+AGG 0.625525 4_2:+63396117 Msa0614360:three_prime_UTR
AACCTGCGGCGAAGGCGTGA+CGG 0.631674 4_2:-63392237 None:intergenic
GTTCGGAGACAACAGAGCGG+AGG 0.635659 4_2:-63392178 None:intergenic
AGCACGAGAATGACTCAATG+AGG 0.657480 4_2:+63396229 Msa0614360:three_prime_UTR
CCGCGCAAATTCTTCTCCAA+CGG 0.664904 4_2:+63392098 Msa0614360:CDS
CGGTGGTGATGAAGGCGAGG+AGG 0.671666 4_2:-63392078 None:intergenic
AGCGGAGGCGATAGTCGATG+AGG 0.674566 4_2:-63392163 None:intergenic
GAAGGAGATATGCTTCAAGA+GGG 0.675308 4_2:-63392779 None:intergenic
AGTGTTGTAGTGGAAATCGA+AGG 0.678543 4_2:-63392797 None:intergenic
TGGCTTTGTAGGTGATGGCG+TGG 0.678911 4_2:-63391947 None:intergenic
TTGGAGAAGAATTTGCGCGG+TGG 0.689867 4_2:-63392095 None:intergenic
CTTTGTAGGTGATGGCGTGG+CGG 0.693253 4_2:-63391944 None:intergenic
GATATGCTTCAAGAGGGCGT+GGG 0.695688 4_2:-63392773 None:intergenic
TGATTGAATTGAGAAGTGTG+GGG 0.704818 4_2:-63392048 None:intergenic
CCGTTGGAGAAGAATTTGCG+CGG 0.729717 4_2:-63392098 None:intergenic
GAAGTTCGGAGACAACAGAG+CGG 0.757254 4_2:-63392181 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAAGTTATCATTATT+TGG - chr4_2:63392578-63392597 None:intergenic 10.0%
!!! ATAATTTTAAACTCTTTTTA+AGG + chr4_2:63395064-63395083 Msa0614360:intron 10.0%
!!! TTTGTATTTTTTTTTCAGTT+GGG - chr4_2:63392402-63392421 None:intergenic 15.0%
!!! TTTTGTATTTTTTTTTCAGT+TGG - chr4_2:63392403-63392422 None:intergenic 15.0%
!! TTTATTCACAAAAAAATACT+TGG - chr4_2:63392695-63392714 None:intergenic 15.0%
!! ACAACTTAAAAAATATTAAG+TGG - chr4_2:63395252-63395271 None:intergenic 15.0%
!!! ATATTGAATTTCATGATTTT+TGG + chr4_2:63395965-63395984 Msa0614360:intron 15.0%
!! TGAAGAAATTTGAATAAGAA+AGG - chr4_2:63391907-63391926 None:intergenic 20.0%
!! ATAGAATATTATATATGCAG+TGG - chr4_2:63392912-63392931 None:intergenic 20.0%
!!! TATATAAACAGACATGTTTT+TGG + chr4_2:63392975-63392994 Msa0614360:intron 20.0%
!!! ATTTCTCTTATTGTGTTTTT+TGG + chr4_2:63393046-63393065 Msa0614360:intron 20.0%
!! CAATTATCTTCAGTTAAATA+TGG - chr4_2:63393373-63393392 None:intergenic 20.0%
!!! TGTTTCTTTTGATCAAATTT+AGG + chr4_2:63393484-63393503 Msa0614360:intron 20.0%
!! TTCAAATTATTATGTTGTTG+AGG + chr4_2:63393890-63393909 Msa0614360:intron 20.0%
!!! CTTTTTTCTTTTATATTCTG+TGG + chr4_2:63394158-63394177 Msa0614360:intron 20.0%
!!! TTCTGATTATTTTTTGTTGT+AGG + chr4_2:63394388-63394407 Msa0614360:intron 20.0%
!! TTACTAATTGTATGTATGTA+TGG + chr4_2:63394518-63394537 Msa0614360:intron 20.0%
!! ACCATAAATAAACAACATTT+AGG - chr4_2:63394623-63394642 None:intergenic 20.0%
!!! CTTCTATATATTTTTACTCT+TGG + chr4_2:63394775-63394794 Msa0614360:intron 20.0%
!! TATAATCTAGTAAACTGTAT+CGG - chr4_2:63394907-63394926 None:intergenic 20.0%
!! TTGTATCTCAAATATTAGAA+AGG + chr4_2:63394999-63395018 Msa0614360:intron 20.0%
!! TCAAATATTAGAAAGGATAA+AGG + chr4_2:63395006-63395025 Msa0614360:intron 20.0%
!!! GAGAAAGATATGTTAATTTT+TGG + chr4_2:63396070-63396089 Msa0614360:three_prime_UTR 20.0%
!!! AAATAAATACAGAAGTATGA+AGG + chr4_2:63396163-63396182 Msa0614360:three_prime_UTR 20.0%
! ACATGAAAAAGTTACAGATA+TGG - chr4_2:63392293-63392312 None:intergenic 25.0%
! CAAGAAATCATGAATTCAAA+AGG - chr4_2:63392329-63392348 None:intergenic 25.0%
!! CTTTTGAATTCATGATTTCT+TGG + chr4_2:63392327-63392346 Msa0614360:intron 25.0%
!! AAATCCTAACTCTGAAAAAT+TGG - chr4_2:63392439-63392458 None:intergenic 25.0%
! GTATATATGTCAATCCAAAA+AGG - chr4_2:63392522-63392541 None:intergenic 25.0%
! TTGCAATACATTTCAATTTG+TGG - chr4_2:63392622-63392641 None:intergenic 25.0%
! CAAATTGAAATGTATTGCAA+TGG + chr4_2:63392622-63392641 Msa0614360:intron 25.0%
! TATAGTATAAATACGAAACC+TGG + chr4_2:63392848-63392867 Msa0614360:intron 25.0%
! ATAGTATAAATACGAAACCT+GGG + chr4_2:63392849-63392868 Msa0614360:intron 25.0%
!!! TTCTGTGTGATTTTTTTAGA+AGG + chr4_2:63392936-63392955 Msa0614360:intron 25.0%
! AAAGAAATAGATAAGACTGA+AGG + chr4_2:63393177-63393196 Msa0614360:intron 25.0%
! CAATCATAAAAAACTGCATT+TGG - chr4_2:63393213-63393232 None:intergenic 25.0%
! TGTGTATATATGAAAGTTCA+AGG - chr4_2:63393243-63393262 None:intergenic 25.0%
! ACAAATTGCATTACTATAGT+TGG - chr4_2:63393331-63393350 None:intergenic 25.0%
!! TTTTGGTATTTAATAGCCAA+TGG + chr4_2:63393454-63393473 Msa0614360:intron 25.0%
! AAAGAAACAAATCTTTCCAT+TGG - chr4_2:63393473-63393492 None:intergenic 25.0%
! CTACTTCAATTTACAATACA+AGG + chr4_2:63393556-63393575 Msa0614360:intron 25.0%
! TTCAATTTACAATACAAGGT+TGG + chr4_2:63393560-63393579 Msa0614360:intron 25.0%
! TCAATTTACAATACAAGGTT+GGG + chr4_2:63393561-63393580 Msa0614360:intron 25.0%
! AAGTTCATATAAAAGTTTGC+AGG + chr4_2:63393801-63393820 Msa0614360:CDS 25.0%
!!! CAGTTTTTTCTACATGAAAT+TGG - chr4_2:63394034-63394053 None:intergenic 25.0%
! AAGCACAAACTTAAACATAT+TGG - chr4_2:63394133-63394152 None:intergenic 25.0%
!! ATTTTTCCTAAACATTGCTT+TGG + chr4_2:63394253-63394272 Msa0614360:intron 25.0%
! AATTGATATACCAAGAATTG+TGG - chr4_2:63394279-63394298 None:intergenic 25.0%
!!! ATTTTTTGTTGTAGGCATTT+CGG + chr4_2:63394396-63394415 Msa0614360:intron 25.0%
! GCAAAAAAATTTAGGTATGA+AGG + chr4_2:63394486-63394505 Msa0614360:intron 25.0%
!! GCCTAAATGTTGTTTATTTA+TGG + chr4_2:63394619-63394638 Msa0614360:intron 25.0%
! TTTATGGTTATGCATTTAAG+AGG + chr4_2:63394635-63394654 Msa0614360:intron 25.0%
! TTATGGTTATGCATTTAAGA+GGG + chr4_2:63394636-63394655 Msa0614360:intron 25.0%
!!! CACTTTTTTAACAACAAAGA+TGG + chr4_2:63394659-63394678 Msa0614360:intron 25.0%
!!! AAAGATGGATTAACCAATTA+GGG + chr4_2:63394674-63394693 Msa0614360:intron 25.0%
! AGAAGGTTTAATCATATTCA+TGG + chr4_2:63394703-63394722 Msa0614360:intron 25.0%
! GAAGGTTTAATCATATTCAT+GGG + chr4_2:63394704-63394723 Msa0614360:intron 25.0%
!! GTGTTTTTCATATGAAATCA+TGG + chr4_2:63394731-63394750 Msa0614360:intron 25.0%
! CTAAAGAAAAATTTGGCTAT+TGG - chr4_2:63394859-63394878 None:intergenic 25.0%
! GTAGGAACTAAAGAAAAATT+TGG - chr4_2:63394866-63394885 None:intergenic 25.0%
! TTGCAATCTTATAAACTGTA+AGG - chr4_2:63394941-63394960 None:intergenic 25.0%
! TTTAATTACATATGCGTTCA+TGG - chr4_2:63395297-63395316 None:intergenic 25.0%
! AATTGAAAGAGACAACTTAA+AGG + chr4_2:63395322-63395341 Msa0614360:intron 25.0%
! TGATCTTACACTTAAGAATT+TGG + chr4_2:63395380-63395399 Msa0614360:intron 25.0%
! TAAAAGGTCAAACACAAAAA+AGG - chr4_2:63395635-63395654 None:intergenic 25.0%
! ATGAAACTTGAGTTCTTAAA+AGG - chr4_2:63395651-63395670 None:intergenic 25.0%
! ATTCAAACTCATTATCTAGA+AGG - chr4_2:63395808-63395827 None:intergenic 25.0%
!!! GATAGAAAGTATGTTTTTTC+TGG + chr4_2:63395835-63395854 Msa0614360:intron 25.0%
! GAAATTCAATATACATGATG+TGG - chr4_2:63395957-63395976 None:intergenic 25.0%
!!! TTGAATTTCATGATTTTTGG+AGG + chr4_2:63395968-63395987 Msa0614360:intron 25.0%
! CATGATTGTCATATATATGT+AGG + chr4_2:63396012-63396031 Msa0614360:intron 25.0%
!!! ATGTTAATTTTTGGAGTTTC+AGG + chr4_2:63396079-63396098 Msa0614360:three_prime_UTR 25.0%
! AAGGAAGATAATTTAGTTGT+TGG + chr4_2:63396182-63396201 Msa0614360:three_prime_UTR 25.0%
! AGGAAGATAATTTAGTTGTT+GGG + chr4_2:63396183-63396202 Msa0614360:three_prime_UTR 25.0%
!! ATGTATAGTTTGTGATACAT+TGG + chr4_2:63396345-63396364 Msa0614360:three_prime_UTR 25.0%
! TTGATTGAATTGAGAAGTGT+GGG - chr4_2:63392052-63392071 None:intergenic 30.0%
TTTGATTGAATTGAGAAGTG+TGG - chr4_2:63392053-63392072 None:intergenic 30.0%
ACATAAAGAAAAGATATGCG+AGG - chr4_2:63392267-63392286 None:intergenic 30.0%
CTGAAAAATTGGAAAGAGAA+TGG - chr4_2:63392428-63392447 None:intergenic 30.0%
!! CTTTCCAATTTTTCAGAGTT+AGG + chr4_2:63392432-63392451 Msa0614360:intron 30.0%
!! AAGTTTGTTGAATTTAACGC+AGG + chr4_2:63392495-63392514 Msa0614360:intron 30.0%
!! AGTTTGTTGAATTTAACGCA+GGG + chr4_2:63392496-63392515 Msa0614360:intron 30.0%
TTGGATTGACATATATACAG+TGG + chr4_2:63392524-63392543 Msa0614360:intron 30.0%
! TAAAACTATCTCAAGAGCAT+TGG + chr4_2:63392658-63392677 Msa0614360:intron 30.0%
TTCACAAAAAAATACTTGGC+AGG - chr4_2:63392691-63392710 None:intergenic 30.0%
ATATGCAGAAAAGCAGAAAA+AGG + chr4_2:63392733-63392752 Msa0614360:intron 30.0%
TAGTATAAATACGAAACCTG+GGG + chr4_2:63392850-63392869 Msa0614360:intron 30.0%
GAATATTATATATGCAGTGG+CGG - chr4_2:63392909-63392928 None:intergenic 30.0%
!! CAAGTTAAAGTATTTTCTCC+AGG - chr4_2:63393017-63393036 None:intergenic 30.0%
!!! GTGTTTTTTGGACATTTGAT+TGG + chr4_2:63393058-63393077 Msa0614360:intron 30.0%
ATAACAGAACAACACTTCTT+TGG - chr4_2:63393113-63393132 None:intergenic 30.0%
TGTTGTTCTGTTATATCGAT+TGG + chr4_2:63393119-63393138 Msa0614360:intron 30.0%
TAAAAAACTGCATTTGGTTG+TGG - chr4_2:63393207-63393226 None:intergenic 30.0%
!! ACTCATTTTAATACCAAGCT+AGG - chr4_2:63393276-63393295 None:intergenic 30.0%
TGAGAGTTGTAATGCATTAT+AGG + chr4_2:63393302-63393321 Msa0614360:intron 30.0%
! TAATGTTGCCTTATCTGTTT+TGG + chr4_2:63393410-63393429 Msa0614360:intron 30.0%
TTAGTGAACCAAAACAGATA+AGG - chr4_2:63393421-63393440 None:intergenic 30.0%
!!! CTAATTCTCTGGTTTTGTTT+TGG + chr4_2:63393437-63393456 Msa0614360:intron 30.0%
!! GTCATTGTGTTTGATTTGTA+TGG + chr4_2:63393522-63393541 Msa0614360:intron 30.0%
!! CAGGTAAAAAGTTCTAAGAA+TGG + chr4_2:63393820-63393839 Msa0614360:intron 30.0%
ACAACATCAATGACAAAATG+AGG - chr4_2:63393953-63393972 None:intergenic 30.0%
TCATGTAGAAAAAACTGATG+TGG + chr4_2:63394037-63394056 Msa0614360:intron 30.0%
CAAACTTAAACATATTGGCA+TGG - chr4_2:63394128-63394147 None:intergenic 30.0%
CTTGAAAAGAACTCAATTGA+AGG - chr4_2:63394229-63394248 None:intergenic 30.0%
! GTATGTTACTTCAATATTCC+AGG - chr4_2:63394453-63394472 None:intergenic 30.0%
GTAACATACTACATGAGTTA+CGG + chr4_2:63394464-63394483 Msa0614360:exon 30.0%
GAGTTACGGCAAAAAAATTT+AGG + chr4_2:63394478-63394497 Msa0614360:three_prime_UTR 30.0%
CATAAATCATTGTGTCTTTG+AGG + chr4_2:63394569-63394588 Msa0614360:intron 30.0%
!! TCTTTGAGGAAGATTTGTTA+AGG + chr4_2:63394583-63394602 Msa0614360:intron 30.0%
!! AGATTTGTTAAGGTTCTTTG+AGG + chr4_2:63394593-63394612 Msa0614360:intron 30.0%
!! CAAAGATGGATTAACCAATT+AGG + chr4_2:63394673-63394692 Msa0614360:intron 30.0%
! ACCAATTAGGGAATTTTAGA+AGG + chr4_2:63394686-63394705 Msa0614360:intron 30.0%
!! ACCTTCTAAAATTCCCTAAT+TGG - chr4_2:63394690-63394709 None:intergenic 30.0%
AGAAAAATTTGGCTATTGGA+GGG - chr4_2:63394855-63394874 None:intergenic 30.0%
AAGAAAAATTTGGCTATTGG+AGG - chr4_2:63394856-63394875 None:intergenic 30.0%
! TTTAGTTCCTACCTTATATG+TGG + chr4_2:63394874-63394893 Msa0614360:intron 30.0%
AGAAAGGATAAAGGATTATG+AGG + chr4_2:63395015-63395034 Msa0614360:intron 30.0%
AGAGATATATGTAGTGTCTT+TGG + chr4_2:63395165-63395184 Msa0614360:intron 30.0%
TATAATGATGAGTTTAGCGT+TGG + chr4_2:63395432-63395451 Msa0614360:intron 30.0%
TTATAAACTCACTACGAAGA+AGG - chr4_2:63395736-63395755 None:intergenic 30.0%
ATAACTAGACGTGAATTTCA+AGG + chr4_2:63395752-63395771 Msa0614360:intron 30.0%
CAAAATCTGTAAGTTCTCAT+TGG - chr4_2:63395888-63395907 None:intergenic 30.0%
AATTTAGTTGTTGGGTATAC+TGG + chr4_2:63396191-63396210 Msa0614360:three_prime_UTR 30.0%
! AAAAAGTGCATATCATGCAA+TGG + chr4_2:63396257-63396276 Msa0614360:three_prime_UTR 30.0%
! TGATTGAATTGAGAAGTGTG+GGG - chr4_2:63392051-63392070 None:intergenic 35.0%
TCAGAGTTAGGATTTGAAGA+TGG + chr4_2:63392444-63392463 Msa0614360:intron 35.0%
GATTGACATATATACAGTGG+CGG + chr4_2:63392527-63392546 Msa0614360:intron 35.0%
CGGATCTTGACTAAAAAAGT+GGG + chr4_2:63392547-63392566 Msa0614360:intron 35.0%
GGATCTTGACTAAAAAAGTG+GGG + chr4_2:63392548-63392567 Msa0614360:intron 35.0%
GATCTTGACTAAAAAAGTGG+GGG + chr4_2:63392549-63392568 Msa0614360:intron 35.0%
ATCTTGACTAAAAAAGTGGG+GGG + chr4_2:63392550-63392569 Msa0614360:intron 35.0%
! ATGTATTGCAATGGTTTGCA+AGG + chr4_2:63392631-63392650 Msa0614360:intron 35.0%
AGTATAAATACGAAACCTGG+GGG + chr4_2:63392851-63392870 Msa0614360:intron 35.0%
! GGTTTGTTTACTTGAAAACC+TGG + chr4_2:63392996-63393015 Msa0614360:intron 35.0%
CATTCTTCAGCCAAAATCAA+TGG - chr4_2:63393148-63393167 None:intergenic 35.0%
AAGGCAACATTAGTCATTTG+TGG - chr4_2:63393402-63393421 None:intergenic 35.0%
!!! GTTTTGGTTCACTAATTCTC+TGG + chr4_2:63393426-63393445 Msa0614360:intron 35.0%
GAACAGAGAGTAATAAGCTT+TGG - chr4_2:63393931-63393950 None:intergenic 35.0%
TTGTGTGAACTTGGGTAAAT+AGG - chr4_2:63394429-63394448 None:intergenic 35.0%
GATTATATAGCCTCATACAC+TGG + chr4_2:63394973-63394992 Msa0614360:intron 35.0%
AATGTCATTGACCTCTACAA+TGG + chr4_2:63395138-63395157 Msa0614360:intron 35.0%
TATCTCTTGTTCCATTGTAG+AGG - chr4_2:63395152-63395171 None:intergenic 35.0%
TATATGTAGTGTCTTTGGCA+AGG + chr4_2:63395170-63395189 Msa0614360:intron 35.0%
TGCTTAAAGGAGAGGTTATA+TGG - chr4_2:63395220-63395239 None:intergenic 35.0%
! CTTCAATTTGCTTAAAGGAG+AGG - chr4_2:63395228-63395247 None:intergenic 35.0%
! GTGGTCTTCAATTTGCTTAA+AGG - chr4_2:63395233-63395252 None:intergenic 35.0%
! TCTGAAGTCATCATTGTAGT+AGG - chr4_2:63395529-63395548 None:intergenic 35.0%
ATCAACAGTAGAAGAATCTG+AGG - chr4_2:63395591-63395610 None:intergenic 35.0%
ACGAAGAAGGTAGAAAATGA+AGG - chr4_2:63395723-63395742 None:intergenic 35.0%
TGAAGTCTCTCAACACAAAT+GGG - chr4_2:63395918-63395937 None:intergenic 35.0%
ATGAAGTCTCTCAACACAAA+TGG - chr4_2:63395919-63395938 None:intergenic 35.0%
AGGTTCACTAATGCAATCTT+TGG + chr4_2:63396032-63396051 Msa0614360:intron 35.0%
TTCACTAATGCAATCTTTGG+AGG + chr4_2:63396035-63396054 Msa0614360:three_prime_UTR 35.0%
! AATTTAACGCAGGGCCTTTT+TGG + chr4_2:63392505-63392524 Msa0614360:intron 40.0%
GCGGATCTTGACTAAAAAAG+TGG + chr4_2:63392546-63392565 Msa0614360:intron 40.0%
AAGCAGAAAAAGGAGACAAG+AGG + chr4_2:63392743-63392762 Msa0614360:CDS 40.0%
GAAGGAGATATGCTTCAAGA+GGG - chr4_2:63392782-63392801 None:intergenic 40.0%
! AGTGTTGTAGTGGAAATCGA+AGG - chr4_2:63392800-63392819 None:intergenic 40.0%
TTCGATTTCCACTACAACAC+TGG + chr4_2:63392799-63392818 Msa0614360:CDS 40.0%
ATATGTCACCAGTGTTGTAG+TGG - chr4_2:63392810-63392829 None:intergenic 40.0%
GTATAAATACGAAACCTGGG+GGG + chr4_2:63392852-63392871 Msa0614360:intron 40.0%
!!! CGATTGGAAGCCATTGATTT+TGG + chr4_2:63393135-63393154 Msa0614360:intron 40.0%
! TGTCATGTGCTGTTATCTGT+GGG + chr4_2:63393710-63393729 Msa0614360:CDS 40.0%
!! TGCTGCTGTTTGTGTTTTGT+GGG + chr4_2:63393736-63393755 Msa0614360:CDS 40.0%
CTGTGGTTCATGAATCAAAC+TGG + chr4_2:63394175-63394194 Msa0614360:intron 40.0%
TTGTGGCCAAAGCAATGTTT+AGG - chr4_2:63394262-63394281 None:intergenic 40.0%
ATTGCTTTGGCCACAATTCT+TGG + chr4_2:63394266-63394285 Msa0614360:intron 40.0%
TTCCAGGATTGTGTGAACTT+GGG - chr4_2:63394437-63394456 None:intergenic 40.0%
ATTCCAGGATTGTGTGAACT+TGG - chr4_2:63394438-63394457 None:intergenic 40.0%
CTCTACACCACATATAAGGT+AGG - chr4_2:63394884-63394903 None:intergenic 40.0%
GATACAACTTCCAGTGTATG+AGG - chr4_2:63394986-63395005 None:intergenic 40.0%
!! ATGAGTTTAGCGTTGGTCTT+TGG + chr4_2:63395439-63395458 Msa0614360:intron 40.0%
ACTGCAAATTCAGAAACTGC+AGG - chr4_2:63395496-63395515 None:intergenic 40.0%
AGATTCTTCTACTCTGAGCA+TGG + chr4_2:63395546-63395565 Msa0614360:intron 40.0%
! CACAAAAAAGGAGTACAGAC+TGG - chr4_2:63395623-63395642 None:intergenic 40.0%
!! TTTTCTGGTGCAATCTACGT+TGG + chr4_2:63395850-63395869 Msa0614360:intron 40.0%
! TTTCTGGTGCAATCTACGTT+GGG + chr4_2:63395851-63395870 Msa0614360:intron 40.0%
!!! GTTTCAGGACTGTTTTTGCA+AGG + chr4_2:63396094-63396113 Msa0614360:three_prime_UTR 40.0%
!!! GACTGTTTTTGCAAGGCATT+TGG + chr4_2:63396101-63396120 Msa0614360:three_prime_UTR 40.0%
AGGCATTTGGCAAACATGTA+TGG + chr4_2:63396114-63396133 Msa0614360:three_prime_UTR 40.0%
CATTTGGCAAACATGTATGG+AGG + chr4_2:63396117-63396136 Msa0614360:three_prime_UTR 40.0%
!! GTTGATGGCTTTGTAGGTGA+TGG - chr4_2:63391955-63391974 None:intergenic 45.0%
AAAGCCATCAACCGTCGTTT+CGG + chr4_2:63391963-63391982 Msa0614360:five_prime_UTR 45.0%
GCAAATTTGTGCCGAAACGA+CGG - chr4_2:63391977-63391996 None:intergenic 45.0%
! CATCGTTGCTGGTTAGTGTT+AGG - chr4_2:63392015-63392034 None:intergenic 45.0%
CATGGGATGAGAGAGAAGTT+CGG - chr4_2:63392198-63392217 None:intergenic 45.0%
ATATGCGAGGATGTAACCTG+CGG - chr4_2:63392254-63392273 None:intergenic 45.0%
CGAAGGAGATATGCTTCAAG+AGG - chr4_2:63392783-63392802 None:intergenic 45.0%
ACACACAACACAACCTAGCT+TGG + chr4_2:63393260-63393279 Msa0614360:intron 45.0%
! CTGTCATGTGCTGTTATCTG+TGG + chr4_2:63393709-63393728 Msa0614360:CDS 45.0%
!! CTGCTGCTGTTTGTGTTTTG+TGG + chr4_2:63393735-63393754 Msa0614360:CDS 45.0%
!! GCTGCTGTTTGTGTTTTGTG+GGG + chr4_2:63393737-63393756 Msa0614360:CDS 45.0%
! TGTGTTTTGTGGGGACAGTT+AGG + chr4_2:63393746-63393765 Msa0614360:CDS 45.0%
! GTGTTTTGTGGGGACAGTTA+GGG + chr4_2:63393747-63393766 Msa0614360:CDS 45.0%
! TGTTTTGTGGGGACAGTTAG+GGG + chr4_2:63393748-63393767 Msa0614360:CDS 45.0%
GGAGTGGTCGACTGTTAATT+TGG + chr4_2:63393769-63393788 Msa0614360:CDS 45.0%
!! GTCGACTGTTAATTTGGTGC+TGG + chr4_2:63393775-63393794 Msa0614360:CDS 45.0%
TACCCAAGTTCACACAATCC+TGG + chr4_2:63394432-63394451 Msa0614360:CDS 45.0%
TCGGCTCTACACCACATATA+AGG - chr4_2:63394888-63394907 None:intergenic 45.0%
GTAGAAGAATCTGAGGACTG+AGG - chr4_2:63395584-63395603 None:intergenic 45.0%
GGCATATTATACGCAAGCAG+TGG - chr4_2:63395702-63395721 None:intergenic 45.0%
AGCACGAGAATGACTCAATG+AGG + chr4_2:63396229-63396248 Msa0614360:three_prime_UTR 45.0%
!! ACGACGGTTGATGGCTTTGT+AGG - chr4_2:63391961-63391980 None:intergenic 50.0%
GAGACAGTGTTCATCGTTGC+TGG - chr4_2:63392026-63392045 None:intergenic 50.0%
! AATTTGCGCGGTGGTGATGA+AGG - chr4_2:63392089-63392108 None:intergenic 50.0%
TTGGAGAAGAATTTGCGCGG+TGG - chr4_2:63392098-63392117 None:intergenic 50.0%
CCGTTGGAGAAGAATTTGCG+CGG - chr4_2:63392101-63392120 None:intergenic 50.0%
CCGCGCAAATTCTTCTCCAA+CGG + chr4_2:63392098-63392117 Msa0614360:CDS 50.0%
GAAGTTCGGAGACAACAGAG+CGG - chr4_2:63392184-63392203 None:intergenic 50.0%
! GATATGCTTCAAGAGGGCGT+GGG - chr4_2:63392776-63392795 None:intergenic 50.0%
AGATATGCTTCAAGAGGGCG+TGG - chr4_2:63392777-63392796 None:intergenic 50.0%
GGATCTTCTCAAGAGCCCAT+GGG - chr4_2:63392888-63392907 None:intergenic 50.0%
! CTCGACGAGGAGATGTTTTG+AGG + chr4_2:63393586-63393605 Msa0614360:intron 50.0%
GCACAAACAGCTCACAACAG+CGG - chr4_2:63393621-63393640 None:intergenic 50.0%
CTGTTGTGAGCTGTTTGTGC+AGG + chr4_2:63393621-63393640 Msa0614360:intron 50.0%
AAAAACGCAGACAGCAGCAC+AGG - chr4_2:63393647-63393666 None:intergenic 50.0%
!!! GTGCTGCTGTCTGCGTTTTT+TGG + chr4_2:63393647-63393666 Msa0614360:CDS 50.0%
TGTCCAAAAACAGCCTCTGC+AGG - chr4_2:63393675-63393694 None:intergenic 50.0%
!! TTGGCTATTGGAGGGTGCTT+GGG - chr4_2:63394847-63394866 None:intergenic 50.0%
!! TTTGGCTATTGGAGGGTGCT+TGG - chr4_2:63394848-63394867 None:intergenic 50.0%
AATCTGAGGACTGAGGACTG+AGG - chr4_2:63395577-63395596 None:intergenic 50.0%
! CGTGGCGGAAGAGAGATTCT+AGG - chr4_2:63391932-63391951 None:intergenic 55.0%
! CTTTGTAGGTGATGGCGTGG+CGG - chr4_2:63391947-63391966 None:intergenic 55.0%
!! TGGCTTTGTAGGTGATGGCG+TGG - chr4_2:63391950-63391969 None:intergenic 55.0%
TGTGCCGAAACGACGGTTGA+TGG - chr4_2:63391970-63391989 None:intergenic 55.0%
CGGATCTTCTCAAGAGCCCA+TGG - chr4_2:63392889-63392908 None:intergenic 55.0%
!!! GCGTTTTTTGGCACCTGCAG+AGG + chr4_2:63393659-63393678 Msa0614360:CDS 55.0%
!! GCACCTGCAGAGGCTGTTTT+TGG + chr4_2:63393669-63393688 Msa0614360:CDS 55.0%
GGTGATGAAGGCGAGGAGGA+CGG - chr4_2:63392077-63392096 None:intergenic 60.0%
CCAACGGCTACGACGTCGAT+CGG + chr4_2:63392114-63392133 Msa0614360:CDS 60.0%
CAACGGCTACGACGTCGATC+GGG + chr4_2:63392115-63392134 Msa0614360:CDS 60.0%
AGCGGAGGCGATAGTCGATG+AGG - chr4_2:63392166-63392185 None:intergenic 60.0%
GTTCGGAGACAACAGAGCGG+AGG - chr4_2:63392181-63392200 None:intergenic 60.0%
AAGGCGGCGATGGTTGGCAT+GGG - chr4_2:63392215-63392234 None:intergenic 60.0%
GAGGATGTAACCTGCGGCGA+AGG - chr4_2:63392248-63392267 None:intergenic 60.0%
TCAAGAGCCCATGGGAGCCA+TGG - chr4_2:63392880-63392899 None:intergenic 60.0%
ACAAGGTTGGGTGCTCGACG+AGG + chr4_2:63393573-63393592 Msa0614360:intron 60.0%
TGTGGGGACAGTTAGGGGAG+TGG + chr4_2:63393753-63393772 Msa0614360:CDS 60.0%
! CGGTGGTGATGAAGGCGAGG+AGG - chr4_2:63392081-63392100 None:intergenic 65.0%
CCGATCGACGTCGTAGCCGT+TGG - chr4_2:63392117-63392136 None:intergenic 65.0%
GAAGGCGGCGATGGTTGGCA+TGG - chr4_2:63392216-63392235 None:intergenic 65.0%
ACGGTGAAGGCGGCGATGGT+TGG - chr4_2:63392221-63392240 None:intergenic 65.0%
! CGAAGGCGTGACGGTGAAGG+CGG - chr4_2:63392231-63392250 None:intergenic 65.0%
! AACCTGCGGCGAAGGCGTGA+CGG - chr4_2:63392240-63392259 None:intergenic 65.0%
ACGAAACCTGGGGGGAGCCA+TGG + chr4_2:63392860-63392879 Msa0614360:intron 65.0%
! GCGCGGTGGTGATGAAGGCG+AGG - chr4_2:63392084-63392103 None:intergenic 70.0%
CGTGACGGTGAAGGCGGCGA+TGG - chr4_2:63392225-63392244 None:intergenic 70.0%
! CGGCGAAGGCGTGACGGTGA+AGG - chr4_2:63392234-63392253 None:intergenic 70.0%
GGGGGAGCCATGGCTCCCAT+GGG + chr4_2:63392870-63392889 Msa0614360:intron 70.0%
CACCGTCACGCCTTCGCCGC+AGG + chr4_2:63392235-63392254 Msa0614360:CDS 75.0%
TGGGAGCCATGGCTCCCCCC+AGG - chr4_2:63392869-63392888 None:intergenic 75.0%
GGGGGGAGCCATGGCTCCCA+TGG + chr4_2:63392869-63392888 Msa0614360:intron 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 63391895 63396416 63391895 ID=Msa0614360;Name=Msa0614360
chr4_2 mRNA 63391895 63396416 63391895 ID=Msa0614360-mRNA-1;Parent=Msa0614360;Name=Msa0614360-mRNA-1;_AED=0.30;_eAED=0.30;_QI=137|0.5|0.6|0.8|0.75|0.6|5|415|180
chr4_2 exon 63391895 63392256 63391895 ID=Msa0614360-mRNA-1:exon:17685;Parent=Msa0614360-mRNA-1
chr4_2 exon 63392741 63392820 63392741 ID=Msa0614360-mRNA-1:exon:17686;Parent=Msa0614360-mRNA-1
chr4_2 exon 63393643 63393822 63393643 ID=Msa0614360-mRNA-1:exon:17687;Parent=Msa0614360-mRNA-1
chr4_2 exon 63394410 63394499 63394410 ID=Msa0614360-mRNA-1:exon:17688;Parent=Msa0614360-mRNA-1
chr4_2 exon 63396034 63396416 63396034 ID=Msa0614360-mRNA-1:exon:17689;Parent=Msa0614360-mRNA-1
chr4_2 five_prime_UTR 63391895 63392031 63391895 ID=Msa0614360-mRNA-1:five_prime_utr;Parent=Msa0614360-mRNA-1
chr4_2 CDS 63392032 63392256 63392032 ID=Msa0614360-mRNA-1:cds;Parent=Msa0614360-mRNA-1
chr4_2 CDS 63392741 63392820 63392741 ID=Msa0614360-mRNA-1:cds;Parent=Msa0614360-mRNA-1
chr4_2 CDS 63393643 63393822 63393643 ID=Msa0614360-mRNA-1:cds;Parent=Msa0614360-mRNA-1
chr4_2 CDS 63394410 63394467 63394410 ID=Msa0614360-mRNA-1:cds;Parent=Msa0614360-mRNA-1
chr4_2 three_prime_UTR 63394468 63394499 63394468 ID=Msa0614360-mRNA-1:three_prime_utr;Parent=Msa0614360-mRNA-1
chr4_2 three_prime_UTR 63396034 63396416 63396034 ID=Msa0614360-mRNA-1:three_prime_utr;Parent=Msa0614360-mRNA-1
Gene Sequence

>Msa0614360

ATGAACACTGTCTCAACCCCACACTTCTCAATTCAATCAAATCCGTCCTCCTCGCCTTCATCACCACCGCGCAAATTCTTCTCCAACGGCTACGACGTCGATCGGGCACAAAACAACAGCTCTCGCATGATCCTCATCGACTATCGCCTCCGCTCTGTTGTCTCCGAACTTCTCTCTCATCCCATGCCAACCATCGCCGCCTTCACCGTCACGCCTTCGCCGCAGAAAAGCAGAAAAAGGAGACAAGAGGAGTCGAACCCACGCCCTCTTGAAGCATATCTCCTTCGATTTCCACTACAACACTGGCCTGTGCTGCTGTCTGCGTTTTTTGGCACCTGCAGAGGCTGTTTTTGGACAATCACGAGCTGCTGCTGTCATGTGCTGTTATCTGTGGGTGCTGCTGCTGTTTGTGTTTTGTGGGGACAGTTAGGGGAGTGGTCGACTGTTAATTTGGTGCTGGTTAAAGTTCATATAAAAGTTTGCAGGCATTTCGGATATGTACCTATTTACCCAAGTTCACACAATCCTGGAATATTGAAGTAA
Protein sequence

>Msa0614360

MNTVSTPHFSIQSNPSSSPSSPPRKFFSNGYDVDRAQNNSSRMILIDYRLRSVVSELLSHPMPTIAAFTVTPSPQKSRKRRQEESNPRPLEAYLLRFPLQHWPVLLSAFFGTCRGCFWTITSCCCHVLLSVGAAAVCVLWGQLGEWSTVNLVLVKVHIKVCRHFGYVPIYPSSHNPGILK*