AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0618410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0618410 MtrunA17_Chr8g0380901 98.347 121 2 0 1 121 1 121 1.25e-86 247
Msa0618410 MtrunA17_Chr8g0380711 77.869 122 26 1 1 121 1 122 6.34e-57 172
Msa0618410 MtrunA17_Chr3g0125791 60.800 125 44 1 1 120 1 125 5.25e-51 157
Msa0618410 MtrunA17_Chr3g0125761 58.268 127 46 2 1 121 1 126 1.84e-49 153
Msa0618410 MtrunA17_Chr2g0301431 55.645 124 51 1 1 120 1 124 1.33e-45 144
Msa0618410 MtrunA17_Chr2g0323021 40.000 115 64 4 9 119 6 119 2.82e-24 92.8
Msa0618410 MtrunA17_Chr7g0253681 38.462 117 68 3 8 120 7 123 3.40e-22 85.5
Msa0618410 MtrunA17_Chr7g0253701 36.066 122 74 3 3 120 1 122 3.64e-21 82.8
Msa0618410 MtrunA17_Chr7g0253671 36.066 122 74 3 3 120 1 122 3.64e-21 82.8
Msa0618410 MtrunA17_Chr1g0177341 39.316 117 67 3 7 119 4 120 6.08e-21 82.4
Msa0618410 MtrunA17_Chr1g0177331 39.316 117 67 3 7 119 4 120 6.08e-21 82.4
Msa0618410 MtrunA17_Chr7g0253731 38.525 122 68 4 1 118 1 119 1.37e-20 81.6
Msa0618410 MtrunA17_Chr8g0335531 42.241 116 59 5 11 119 5 119 1.79e-20 81.6
Msa0618410 MtrunA17_Chr7g0253761 39.837 123 69 3 1 120 1 121 3.02e-20 80.9
Msa0618410 MtrunA17_Chr1g0205701 33.621 116 68 3 11 119 8 121 6.97e-20 80.1
Msa0618410 MtrunA17_Chr7g0253641 39.344 122 69 3 1 119 1 120 1.21e-19 80.5
Msa0618410 MtrunA17_Chr5g0394321 33.043 115 72 2 10 119 8 122 5.50e-19 77.8
Msa0618410 MtrunA17_Chr1g0211101 36.752 117 68 4 8 120 15 129 5.86e-19 77.8
Msa0618410 MtrunA17_Chr8g0335571 39.655 116 62 5 11 119 5 119 5.87e-19 78.2
Msa0618410 MtrunA17_Chr4g0064041 40.000 100 53 2 27 119 190 289 7.49e-18 77.4
Msa0618410 MtrunA17_Chr4g0064041 36.441 118 67 3 9 119 11 127 1.69e-15 70.9
Msa0618410 MtrunA17_Chr6g0481491 35.106 94 57 3 28 118 28 120 7.19e-17 72.8
Msa0618410 MtrunA17_Chr2g0306461 32.258 124 77 3 1 118 1 123 9.42e-17 72.4
Msa0618410 MtrunA17_Chr6g0483981 40.777 103 52 2 25 118 24 126 1.15e-16 74.7
Msa0618410 MtrunA17_Chr7g0253631 38.525 122 70 3 1 119 1 120 1.31e-15 70.1
Msa0618410 MtrunA17_Chr3g0132701 38.596 114 64 5 11 118 7 120 1.72e-15 69.3
Msa0618410 MtrunA17_Chr6g0459441 36.000 100 59 4 25 120 23 121 2.29e-15 68.6
Msa0618410 MtrunA17_Chr3g0132431 39.823 113 58 6 11 116 7 116 5.20e-15 67.4
Msa0618410 MtrunA17_Chr6g0453801 30.952 126 79 3 1 120 8 131 1.92e-14 65.9
Msa0618410 MtrunA17_Chr3g0132511 37.719 114 65 5 11 118 7 120 2.31e-14 66.6
Msa0618410 MtrunA17_Chr8g0387804 33.621 116 63 4 13 120 18 127 8.74e-14 63.2
Msa0618410 MtrunA17_Chr6g0453921 34.454 119 73 3 7 120 16 134 4.19e-13 62.4
Msa0618410 MtrunA17_Chr3g0132411 36.752 117 68 5 7 118 42 157 1.40e-12 60.8
Msa0618410 MtrunA17_Chr1g0150131 31.667 120 73 3 7 118 5 123 1.52e-12 61.2
Msa0618410 MtrunA17_Chr4g0064031 33.981 103 56 4 24 115 24 125 6.06e-12 58.9
Msa0618410 MtrunA17_Chr8g0360371 31.304 115 74 2 9 118 5 119 1.10e-11 60.1
Msa0618410 MtrunA17_Chr4g0001571 31.250 96 64 1 25 118 26 121 2.73e-11 57.4
Msa0618410 MtrunA17_Chr6g0453791 32.292 96 62 2 28 120 31 126 8.01e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0618410 AT2G02850.1 60.156 128 43 1 1 120 1 128 1.78e-52 161
Msa0618410 AT3G17675.1 36.066 122 73 3 5 121 7 128 5.73e-22 84.3
Msa0618410 AT1G17800.1 41.176 119 64 5 8 120 18 136 6.69e-21 82.0
Msa0618410 AT2G32300.1 39.796 98 56 2 25 119 23 120 2.85e-20 83.2
Msa0618410 AT2G32300.2 39.796 98 56 2 25 119 53 150 4.52e-20 83.2
Msa0618410 AT3G60270.1 42.857 98 52 3 25 119 23 119 6.01e-20 80.9
Msa0618410 AT3G27200.1 36.975 119 70 4 7 121 5 122 3.83e-19 78.6
Msa0618410 AT5G26330.1 35.652 115 64 4 12 119 9 120 2.32e-18 76.6
Msa0618410 AT3G60280.1 44.898 98 48 4 25 118 21 116 4.07e-18 76.6
Msa0618410 AT2G44790.1 43.137 102 48 5 23 119 27 123 1.21e-16 72.4
Msa0618410 AT2G31050.1 33.071 127 74 4 1 119 1 124 1.36e-16 72.4
Msa0618410 AT2G26720.1 33.333 99 62 2 26 120 27 125 4.42e-16 71.2
Msa0618410 AT5G07475.1 34.821 112 69 3 11 119 13 123 7.58e-16 70.1
Msa0618410 AT2G25060.1 33.613 119 67 3 8 118 15 129 2.26e-13 63.5
Msa0618410 AT3G20570.1 31.858 113 70 3 12 118 15 126 2.51e-13 63.9
Msa0618410 AT4G12880.1 30.081 123 79 4 3 118 1 123 2.81e-12 59.7
Msa0618410 AT1G22480.1 38.793 116 61 5 11 121 4 114 8.63e-12 59.3
Msa0618410 AT4G31840.1 31.667 120 70 4 11 118 6 125 8.87e-12 59.3
Msa0618410 AT1G48940.1 32.308 130 66 8 6 120 3 125 1.14e-11 58.9
Msa0618410 AT3G18590.1 31.496 127 72 7 6 120 3 126 1.20e-11 59.3
Msa0618410 AT4G27520.1 33.913 115 67 3 9 118 16 126 1.32e-11 60.5
Msa0618410 AT5G53870.1 30.952 126 71 5 7 118 2 125 6.29e-11 58.5
Msa0618410 AT2G27035.1 29.661 118 77 3 8 120 8 124 7.06e-11 56.6

Find 68 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCCACTTATTTAATTTA+AGG 0.058031 4_2:-69385957 None:intergenic
ATTTACCACTGTTATTATTT+TGG 0.148598 4_2:+69386122 Msa0618410:three_prime_UTR
TGAAATAAACATAATGAGTT+TGG 0.208957 4_2:+69384943 Msa0618410:five_prime_UTR
GATTTGAAGGTAAGTGAATT+TGG 0.221004 4_2:-69385104 None:intergenic
AAGTTGAACACTACATTATT+AGG 0.282780 4_2:-69385832 None:intergenic
TAAATACTTCTTCATATTGC+AGG 0.322954 4_2:-69384998 None:intergenic
AGGCCAACCAACAGTGTTAA+AGG 0.339711 4_2:-69385277 None:intergenic
ACCTACACTGTTGGAGGTCC+TGG 0.340570 4_2:+69385246 Msa0618410:CDS
CACCCATTAAGATTTCTTGC+AGG 0.368254 4_2:+69385034 Msa0618410:five_prime_UTR
TAAAAGTTAAGCAGCATTGA+TGG 0.375502 4_2:-69385792 None:intergenic
GAAATAAACATAATGAGTTT+GGG 0.380221 4_2:+69384944 Msa0618410:five_prime_UTR
GTAGTTCCCTTCAAAACAAT+TGG 0.383981 4_2:-69386088 None:intergenic
AGGCCTGCAAGAAATCTTAA+TGG 0.392067 4_2:-69385037 None:intergenic
TTATCCTTAAATTAAATAAG+TGG 0.400701 4_2:+69385953 Msa0618410:three_prime_UTR
CTACTTCATTTGCAACTTTG+TGG 0.404030 4_2:+69385742 Msa0618410:CDS
TGTGAAAGAAAGGGATTTGA+AGG 0.430605 4_2:-69385117 None:intergenic
CTACTATATGTTAGTCATCA+TGG 0.431538 4_2:+69386003 Msa0618410:three_prime_UTR
GCTAGAAAACTGTGAAAGAA+AGG 0.441460 4_2:-69385127 None:intergenic
TACTTCATTTGCAACTTTGT+GGG 0.443056 4_2:+69385743 Msa0618410:CDS
GGAGCAAAAGTGTATAGGTC+AGG 0.453219 4_2:+69385686 Msa0618410:CDS
TAACACTGTTGGTTGGCCTA+AGG 0.454454 4_2:+69385281 Msa0618410:CDS
TTTGTGGGTCACTGCGAGTC+CGG 0.457246 4_2:+69385758 Msa0618410:CDS
CTAGTAGAGAAACATGGCTC+TGG 0.459629 4_2:+69385155 Msa0618410:exon
AGGATCAGATAAGGCTTGCT+AGG 0.465568 4_2:+69385711 Msa0618410:CDS
AACACTGTTGGTTGGCCTAA+GGG 0.479850 4_2:+69385282 Msa0618410:CDS
GGACCTTTAACACTGTTGGT+TGG 0.480078 4_2:+69385274 Msa0618410:CDS
TTGGGCAGACCCACGTGTCT+TGG 0.480551 4_2:+69384962 Msa0618410:five_prime_UTR
CTAGAAAACTGTGAAAGAAA+GGG 0.482984 4_2:-69385126 None:intergenic
AGTACACATGCATAAAGAAC+AGG 0.484146 4_2:-69385910 None:intergenic
AGAAACATGGCTCTGGGAAG+AGG 0.491666 4_2:+69385162 Msa0618410:exon
GGCCTGCAAGAAATCTTAAT+GGG 0.503092 4_2:-69385036 None:intergenic
GAATTTGGAATGATGTGATA+AGG 0.505362 4_2:-69385089 None:intergenic
GTGTTAAAGGTCCAACCTCC+AGG 0.508532 4_2:-69385264 None:intergenic
ATATGATTTCTGGGTCTCTC+CGG 0.514100 4_2:+69386050 Msa0618410:three_prime_UTR
GCTGCTACCTACACTGTTGG+AGG 0.517016 4_2:+69385240 Msa0618410:CDS
CAAGAGGAGCAAAAGTGTAT+AGG 0.522207 4_2:+69385681 Msa0618410:CDS
GCCACCACATTGTGAGCTGA+TGG 0.525344 4_2:-69385623 None:intergenic
CATGCTGCTACCTACACTGT+TGG 0.528640 4_2:+69385237 Msa0618410:CDS
CTGTTGGAGGTCCTGGAGGT+TGG 0.534102 4_2:+69385253 Msa0618410:CDS
AAGAAATCTTAATGGGTGGT+TGG 0.538997 4_2:-69385029 None:intergenic
GGTTGGACCTTTAACACTGT+TGG 0.539100 4_2:+69385270 Msa0618410:CDS
TTTAGGGCTGGTGACACACT+TGG 0.539527 4_2:+69385312 Msa0618410:CDS
TCTGGGAAGAGGCAGTGCAT+TGG 0.544752 4_2:+69385173 Msa0618410:CDS
AAATTAAATAAGTGGATAGG+TGG 0.544930 4_2:+69385961 Msa0618410:three_prime_UTR
GGATCAGATAAGGCTTGCTA+GGG 0.550417 4_2:+69385712 Msa0618410:CDS
CTTAAATTAAATAAGTGGAT+AGG 0.562680 4_2:+69385958 Msa0618410:three_prime_UTR
TTGGTTCGCTACATAACATC+CGG 0.565357 4_2:-69386069 None:intergenic
AATTAAATAAGTGGATAGGT+GGG 0.568337 4_2:+69385962 Msa0618410:three_prime_UTR
GGTCAGGAAAGGATCAGATA+AGG 0.577849 4_2:+69385702 Msa0618410:CDS
TAGTAGAGAAACATGGCTCT+GGG 0.578678 4_2:+69385156 Msa0618410:exon
TGAGGAGTGAGTGAAGAGGC+AGG 0.584806 4_2:-69385057 None:intergenic
TAAAGAACAGGTACAACAAG+AGG 0.594279 4_2:-69385898 None:intergenic
AAAAGTGTATAGGTCAGGAA+AGG 0.608610 4_2:+69385691 Msa0618410:CDS
TACACTGTTGGAGGTCCTGG+AGG 0.611395 4_2:+69385249 Msa0618410:CDS
CAAATACTAGTAGAGAAACA+TGG 0.613492 4_2:+69385149 Msa0618410:exon
GTGGTGGCAGTGAACAAAGG+AGG 0.631663 4_2:+69385638 Msa0618410:CDS
GATAGTTGCAAGACACCAAG+AGG 0.632266 4_2:+69385665 Msa0618410:CDS
AATGTGGTGGCAGTGAACAA+AGG 0.638717 4_2:+69385635 Msa0618410:CDS
TCCATCAGCTCACAATGTGG+TGG 0.645459 4_2:+69385622 Msa0618410:CDS
TGGTGGCAGTGAACAAAGGA+GGG 0.647438 4_2:+69385639 Msa0618410:CDS
TAATTGAGGAGTGAGTGAAG+AGG 0.656376 4_2:-69385061 None:intergenic
CTGCAAGAAATCTTAATGGG+TGG 0.656921 4_2:-69385033 None:intergenic
ACTCATCTGACCAAGACACG+TGG 0.661487 4_2:-69384972 None:intergenic
TTAAGCCAAAATAATAACAG+TGG 0.665899 4_2:-69386127 None:intergenic
TCCAGGACCTCCAACAGTGT+AGG 0.688043 4_2:-69385247 None:intergenic
GATCAGATAAGGCTTGCTAG+GGG 0.699536 4_2:+69385713 Msa0618410:CDS
TAGTCCATCAGCTCACAATG+TGG 0.719459 4_2:+69385619 Msa0618410:CDS
CTCATCTGACCAAGACACGT+GGG 0.723003 4_2:-69384971 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAATAAGACTAATGTT+TGG - chr4_2:69385405-69385424 None:intergenic 15.0%
!! TTATCCTTAAATTAAATAAG+TGG + chr4_2:69385953-69385972 Msa0618410:three_prime_UTR 15.0%
!! TTATAAATAAGACTAATGTT+TGG - chr4_2:69385405-69385424 None:intergenic 15.0%
!! TTATCCTTAAATTAAATAAG+TGG + chr4_2:69385953-69385972 Msa0618410:three_prime_UTR 15.0%
!! TGAAATAAACATAATGAGTT+TGG + chr4_2:69384943-69384962 Msa0618410:five_prime_UTR 20.0%
!! GAAATAAACATAATGAGTTT+GGG + chr4_2:69384944-69384963 Msa0618410:five_prime_UTR 20.0%
!! TAATAACATTCTTTAACGAT+TGG - chr4_2:69385355-69385374 None:intergenic 20.0%
!! CATTCAATTAATGATGTATA+TGG + chr4_2:69385554-69385573 Msa0618410:intron 20.0%
!!! CTATCCACTTATTTAATTTA+AGG - chr4_2:69385960-69385979 None:intergenic 20.0%
!! CTTAAATTAAATAAGTGGAT+AGG + chr4_2:69385958-69385977 Msa0618410:three_prime_UTR 20.0%
!!! ATTTTGTGTAATATGATTTC+TGG + chr4_2:69386040-69386059 Msa0618410:three_prime_UTR 20.0%
!!! TTTTGTGTAATATGATTTCT+GGG + chr4_2:69386041-69386060 Msa0618410:three_prime_UTR 20.0%
!!! ATTTACCACTGTTATTATTT+TGG + chr4_2:69386122-69386141 Msa0618410:three_prime_UTR 20.0%
!! TGAAATAAACATAATGAGTT+TGG + chr4_2:69384943-69384962 Msa0618410:five_prime_UTR 20.0%
!! GAAATAAACATAATGAGTTT+GGG + chr4_2:69384944-69384963 Msa0618410:five_prime_UTR 20.0%
!! TAATAACATTCTTTAACGAT+TGG - chr4_2:69385355-69385374 None:intergenic 20.0%
!! CATTCAATTAATGATGTATA+TGG + chr4_2:69385554-69385573 Msa0618410:intron 20.0%
!!! CTATCCACTTATTTAATTTA+AGG - chr4_2:69385960-69385979 None:intergenic 20.0%
!! CTTAAATTAAATAAGTGGAT+AGG + chr4_2:69385958-69385977 Msa0618410:three_prime_UTR 20.0%
!!! ATTTTGTGTAATATGATTTC+TGG + chr4_2:69386040-69386059 Msa0618410:three_prime_UTR 20.0%
!!! TTTTGTGTAATATGATTTCT+GGG + chr4_2:69386041-69386060 Msa0618410:three_prime_UTR 20.0%
!!! ATTTACCACTGTTATTATTT+TGG + chr4_2:69386122-69386141 Msa0618410:three_prime_UTR 20.0%
! TAAATACTTCTTCATATTGC+AGG - chr4_2:69385001-69385020 None:intergenic 25.0%
!!! GTGATAAGGTTTTATAATTG+AGG - chr4_2:69385078-69385097 None:intergenic 25.0%
! AAAAACAACAGTGTTAAAGT+GGG - chr4_2:69385441-69385460 None:intergenic 25.0%
! AAAAAACAACAGTGTTAAAG+TGG - chr4_2:69385442-69385461 None:intergenic 25.0%
! AAGTTGAACACTACATTATT+AGG - chr4_2:69385835-69385854 None:intergenic 25.0%
! AAATTAAATAAGTGGATAGG+TGG + chr4_2:69385961-69385980 Msa0618410:three_prime_UTR 25.0%
! AATTAAATAAGTGGATAGGT+GGG + chr4_2:69385962-69385981 Msa0618410:three_prime_UTR 25.0%
! TTAAGCCAAAATAATAACAG+TGG - chr4_2:69386130-69386149 None:intergenic 25.0%
! TAAATACTTCTTCATATTGC+AGG - chr4_2:69385001-69385020 None:intergenic 25.0%
!!! GTGATAAGGTTTTATAATTG+AGG - chr4_2:69385078-69385097 None:intergenic 25.0%
! AAAAACAACAGTGTTAAAGT+GGG - chr4_2:69385441-69385460 None:intergenic 25.0%
! AAAAAACAACAGTGTTAAAG+TGG - chr4_2:69385442-69385461 None:intergenic 25.0%
! AAGTTGAACACTACATTATT+AGG - chr4_2:69385835-69385854 None:intergenic 25.0%
! AAATTAAATAAGTGGATAGG+TGG + chr4_2:69385961-69385980 Msa0618410:three_prime_UTR 25.0%
! AATTAAATAAGTGGATAGGT+GGG + chr4_2:69385962-69385981 Msa0618410:three_prime_UTR 25.0%
! TTAAGCCAAAATAATAACAG+TGG - chr4_2:69386130-69386149 None:intergenic 25.0%
! GAATTTGGAATGATGTGATA+AGG - chr4_2:69385092-69385111 None:intergenic 30.0%
GATTTGAAGGTAAGTGAATT+TGG - chr4_2:69385107-69385126 None:intergenic 30.0%
CTAGAAAACTGTGAAAGAAA+GGG - chr4_2:69385129-69385148 None:intergenic 30.0%
CAAATACTAGTAGAGAAACA+TGG + chr4_2:69385149-69385168 Msa0618410:exon 30.0%
TCTTCAAAACTCTCTTTCAT+CGG + chr4_2:69385474-69385493 Msa0618410:intron 30.0%
TACTTCATTTGCAACTTTGT+GGG + chr4_2:69385743-69385762 Msa0618410:CDS 30.0%
!! ATTGATGGCAATTTTCATTC+CGG - chr4_2:69385780-69385799 None:intergenic 30.0%
! TAAAAGTTAAGCAGCATTGA+TGG - chr4_2:69385795-69385814 None:intergenic 30.0%
CTACTATATGTTAGTCATCA+TGG + chr4_2:69386003-69386022 Msa0618410:three_prime_UTR 30.0%
! GAATTTGGAATGATGTGATA+AGG - chr4_2:69385092-69385111 None:intergenic 30.0%
GATTTGAAGGTAAGTGAATT+TGG - chr4_2:69385107-69385126 None:intergenic 30.0%
CTAGAAAACTGTGAAAGAAA+GGG - chr4_2:69385129-69385148 None:intergenic 30.0%
CAAATACTAGTAGAGAAACA+TGG + chr4_2:69385149-69385168 Msa0618410:exon 30.0%
TCTTCAAAACTCTCTTTCAT+CGG + chr4_2:69385474-69385493 Msa0618410:intron 30.0%
TACTTCATTTGCAACTTTGT+GGG + chr4_2:69385743-69385762 Msa0618410:CDS 30.0%
!! ATTGATGGCAATTTTCATTC+CGG - chr4_2:69385780-69385799 None:intergenic 30.0%
! TAAAAGTTAAGCAGCATTGA+TGG - chr4_2:69385795-69385814 None:intergenic 30.0%
CTACTATATGTTAGTCATCA+TGG + chr4_2:69386003-69386022 Msa0618410:three_prime_UTR 30.0%
! AAGAAATCTTAATGGGTGGT+TGG - chr4_2:69385032-69385051 None:intergenic 35.0%
TGTGAAAGAAAGGGATTTGA+AGG - chr4_2:69385120-69385139 None:intergenic 35.0%
GCTAGAAAACTGTGAAAGAA+AGG - chr4_2:69385130-69385149 None:intergenic 35.0%
! AAAAGTGTATAGGTCAGGAA+AGG + chr4_2:69385691-69385710 Msa0618410:CDS 35.0%
CTACTTCATTTGCAACTTTG+TGG + chr4_2:69385742-69385761 Msa0618410:CDS 35.0%
TAAAGAACAGGTACAACAAG+AGG - chr4_2:69385901-69385920 None:intergenic 35.0%
AGTACACATGCATAAAGAAC+AGG - chr4_2:69385913-69385932 None:intergenic 35.0%
!!! TAGCGAACCAATTGTTTTGA+AGG + chr4_2:69386081-69386100 Msa0618410:three_prime_UTR 35.0%
!!! AGCGAACCAATTGTTTTGAA+GGG + chr4_2:69386082-69386101 Msa0618410:three_prime_UTR 35.0%
GTAGTTCCCTTCAAAACAAT+TGG - chr4_2:69386091-69386110 None:intergenic 35.0%
! AAGAAATCTTAATGGGTGGT+TGG - chr4_2:69385032-69385051 None:intergenic 35.0%
TGTGAAAGAAAGGGATTTGA+AGG - chr4_2:69385120-69385139 None:intergenic 35.0%
GCTAGAAAACTGTGAAAGAA+AGG - chr4_2:69385130-69385149 None:intergenic 35.0%
! AAAAGTGTATAGGTCAGGAA+AGG + chr4_2:69385691-69385710 Msa0618410:CDS 35.0%
CTACTTCATTTGCAACTTTG+TGG + chr4_2:69385742-69385761 Msa0618410:CDS 35.0%
TAAAGAACAGGTACAACAAG+AGG - chr4_2:69385901-69385920 None:intergenic 35.0%
AGTACACATGCATAAAGAAC+AGG - chr4_2:69385913-69385932 None:intergenic 35.0%
!!! TAGCGAACCAATTGTTTTGA+AGG + chr4_2:69386081-69386100 Msa0618410:three_prime_UTR 35.0%
!!! AGCGAACCAATTGTTTTGAA+GGG + chr4_2:69386082-69386101 Msa0618410:three_prime_UTR 35.0%
GTAGTTCCCTTCAAAACAAT+TGG - chr4_2:69386091-69386110 None:intergenic 35.0%
CTGCAAGAAATCTTAATGGG+TGG - chr4_2:69385036-69385055 None:intergenic 40.0%
CACCCATTAAGATTTCTTGC+AGG + chr4_2:69385034-69385053 Msa0618410:five_prime_UTR 40.0%
GGCCTGCAAGAAATCTTAAT+GGG - chr4_2:69385039-69385058 None:intergenic 40.0%
AGGCCTGCAAGAAATCTTAA+TGG - chr4_2:69385040-69385059 None:intergenic 40.0%
TAATTGAGGAGTGAGTGAAG+AGG - chr4_2:69385064-69385083 None:intergenic 40.0%
TAGTAGAGAAACATGGCTCT+GGG + chr4_2:69385156-69385175 Msa0618410:exon 40.0%
! GCCTAAGGGAAAACGTTTTA+GGG + chr4_2:69385296-69385315 Msa0618410:CDS 40.0%
! CTATACACTTTTGCTCCTCT+TGG - chr4_2:69385683-69385702 None:intergenic 40.0%
! CAAGAGGAGCAAAAGTGTAT+AGG + chr4_2:69385681-69385700 Msa0618410:CDS 40.0%
! ATATGATTTCTGGGTCTCTC+CGG + chr4_2:69386050-69386069 Msa0618410:three_prime_UTR 40.0%
! TTGGTTCGCTACATAACATC+CGG - chr4_2:69386072-69386091 None:intergenic 40.0%
CTGCAAGAAATCTTAATGGG+TGG - chr4_2:69385036-69385055 None:intergenic 40.0%
CACCCATTAAGATTTCTTGC+AGG + chr4_2:69385034-69385053 Msa0618410:five_prime_UTR 40.0%
GGCCTGCAAGAAATCTTAAT+GGG - chr4_2:69385039-69385058 None:intergenic 40.0%
AGGCCTGCAAGAAATCTTAA+TGG - chr4_2:69385040-69385059 None:intergenic 40.0%
TAATTGAGGAGTGAGTGAAG+AGG - chr4_2:69385064-69385083 None:intergenic 40.0%
TAGTAGAGAAACATGGCTCT+GGG + chr4_2:69385156-69385175 Msa0618410:exon 40.0%
! GCCTAAGGGAAAACGTTTTA+GGG + chr4_2:69385296-69385315 Msa0618410:CDS 40.0%
! CTATACACTTTTGCTCCTCT+TGG - chr4_2:69385683-69385702 None:intergenic 40.0%
! CAAGAGGAGCAAAAGTGTAT+AGG + chr4_2:69385681-69385700 Msa0618410:CDS 40.0%
! ATATGATTTCTGGGTCTCTC+CGG + chr4_2:69386050-69386069 Msa0618410:three_prime_UTR 40.0%
! TTGGTTCGCTACATAACATC+CGG - chr4_2:69386072-69386091 None:intergenic 40.0%
CTAGTAGAGAAACATGGCTC+TGG + chr4_2:69385155-69385174 Msa0618410:exon 45.0%
GGTTGGACCTTTAACACTGT+TGG + chr4_2:69385270-69385289 Msa0618410:CDS 45.0%
GGACCTTTAACACTGTTGGT+TGG + chr4_2:69385274-69385293 Msa0618410:CDS 45.0%
AGGCCAACCAACAGTGTTAA+AGG - chr4_2:69385280-69385299 None:intergenic 45.0%
!! TAACACTGTTGGTTGGCCTA+AGG + chr4_2:69385281-69385300 Msa0618410:CDS 45.0%
!! AACACTGTTGGTTGGCCTAA+GGG + chr4_2:69385282-69385301 Msa0618410:CDS 45.0%
! GGCCTAAGGGAAAACGTTTT+AGG + chr4_2:69385295-69385314 Msa0618410:CDS 45.0%
! GCCCTAAAACGTTTTCCCTT+AGG - chr4_2:69385300-69385319 None:intergenic 45.0%
!!! AAGGGAAAACGTTTTAGGGC+TGG + chr4_2:69385300-69385319 Msa0618410:CDS 45.0%
TAGTCCATCAGCTCACAATG+TGG + chr4_2:69385619-69385638 Msa0618410:CDS 45.0%
AATGTGGTGGCAGTGAACAA+AGG + chr4_2:69385635-69385654 Msa0618410:CDS 45.0%
GATAGTTGCAAGACACCAAG+AGG + chr4_2:69385665-69385684 Msa0618410:CDS 45.0%
! GGAGCAAAAGTGTATAGGTC+AGG + chr4_2:69385686-69385705 Msa0618410:CDS 45.0%
GGTCAGGAAAGGATCAGATA+AGG + chr4_2:69385702-69385721 Msa0618410:CDS 45.0%
! AGGATCAGATAAGGCTTGCT+AGG + chr4_2:69385711-69385730 Msa0618410:CDS 45.0%
!! GGATCAGATAAGGCTTGCTA+GGG + chr4_2:69385712-69385731 Msa0618410:CDS 45.0%
!! GATCAGATAAGGCTTGCTAG+GGG + chr4_2:69385713-69385732 Msa0618410:CDS 45.0%
CTAGTAGAGAAACATGGCTC+TGG + chr4_2:69385155-69385174 Msa0618410:exon 45.0%
GGTTGGACCTTTAACACTGT+TGG + chr4_2:69385270-69385289 Msa0618410:CDS 45.0%
GGACCTTTAACACTGTTGGT+TGG + chr4_2:69385274-69385293 Msa0618410:CDS 45.0%
AGGCCAACCAACAGTGTTAA+AGG - chr4_2:69385280-69385299 None:intergenic 45.0%
!! TAACACTGTTGGTTGGCCTA+AGG + chr4_2:69385281-69385300 Msa0618410:CDS 45.0%
!! AACACTGTTGGTTGGCCTAA+GGG + chr4_2:69385282-69385301 Msa0618410:CDS 45.0%
! GGCCTAAGGGAAAACGTTTT+AGG + chr4_2:69385295-69385314 Msa0618410:CDS 45.0%
! GCCCTAAAACGTTTTCCCTT+AGG - chr4_2:69385300-69385319 None:intergenic 45.0%
!!! AAGGGAAAACGTTTTAGGGC+TGG + chr4_2:69385300-69385319 Msa0618410:CDS 45.0%
TAGTCCATCAGCTCACAATG+TGG + chr4_2:69385619-69385638 Msa0618410:CDS 45.0%
AATGTGGTGGCAGTGAACAA+AGG + chr4_2:69385635-69385654 Msa0618410:CDS 45.0%
GATAGTTGCAAGACACCAAG+AGG + chr4_2:69385665-69385684 Msa0618410:CDS 45.0%
! GGAGCAAAAGTGTATAGGTC+AGG + chr4_2:69385686-69385705 Msa0618410:CDS 45.0%
GGTCAGGAAAGGATCAGATA+AGG + chr4_2:69385702-69385721 Msa0618410:CDS 45.0%
! AGGATCAGATAAGGCTTGCT+AGG + chr4_2:69385711-69385730 Msa0618410:CDS 45.0%
!! GGATCAGATAAGGCTTGCTA+GGG + chr4_2:69385712-69385731 Msa0618410:CDS 45.0%
!! GATCAGATAAGGCTTGCTAG+GGG + chr4_2:69385713-69385732 Msa0618410:CDS 45.0%
CTCATCTGACCAAGACACGT+GGG - chr4_2:69384974-69384993 None:intergenic 50.0%
ACTCATCTGACCAAGACACG+TGG - chr4_2:69384975-69384994 None:intergenic 50.0%
!! AGAAACATGGCTCTGGGAAG+AGG + chr4_2:69385162-69385181 Msa0618410:exon 50.0%
CATGCTGCTACCTACACTGT+TGG + chr4_2:69385237-69385256 Msa0618410:CDS 50.0%
GTGTTAAAGGTCCAACCTCC+AGG - chr4_2:69385267-69385286 None:intergenic 50.0%
! TTTAGGGCTGGTGACACACT+TGG + chr4_2:69385312-69385331 Msa0618410:CDS 50.0%
TCCATCAGCTCACAATGTGG+TGG + chr4_2:69385622-69385641 Msa0618410:CDS 50.0%
TGGTGGCAGTGAACAAAGGA+GGG + chr4_2:69385639-69385658 Msa0618410:CDS 50.0%
CTCATCTGACCAAGACACGT+GGG - chr4_2:69384974-69384993 None:intergenic 50.0%
ACTCATCTGACCAAGACACG+TGG - chr4_2:69384975-69384994 None:intergenic 50.0%
!! AGAAACATGGCTCTGGGAAG+AGG + chr4_2:69385162-69385181 Msa0618410:exon 50.0%
CATGCTGCTACCTACACTGT+TGG + chr4_2:69385237-69385256 Msa0618410:CDS 50.0%
GTGTTAAAGGTCCAACCTCC+AGG - chr4_2:69385267-69385286 None:intergenic 50.0%
! TTTAGGGCTGGTGACACACT+TGG + chr4_2:69385312-69385331 Msa0618410:CDS 50.0%
TCCATCAGCTCACAATGTGG+TGG + chr4_2:69385622-69385641 Msa0618410:CDS 50.0%
TGGTGGCAGTGAACAAAGGA+GGG + chr4_2:69385639-69385658 Msa0618410:CDS 50.0%
TGAGGAGTGAGTGAAGAGGC+AGG - chr4_2:69385060-69385079 None:intergenic 55.0%
TCTGGGAAGAGGCAGTGCAT+TGG + chr4_2:69385173-69385192 Msa0618410:CDS 55.0%
GCTGCTACCTACACTGTTGG+AGG + chr4_2:69385240-69385259 Msa0618410:CDS 55.0%
ACCTACACTGTTGGAGGTCC+TGG + chr4_2:69385246-69385265 Msa0618410:CDS 55.0%
TCCAGGACCTCCAACAGTGT+AGG - chr4_2:69385250-69385269 None:intergenic 55.0%
TACACTGTTGGAGGTCCTGG+AGG + chr4_2:69385249-69385268 Msa0618410:CDS 55.0%
GCCACCACATTGTGAGCTGA+TGG - chr4_2:69385626-69385645 None:intergenic 55.0%
GTGGTGGCAGTGAACAAAGG+AGG + chr4_2:69385638-69385657 Msa0618410:CDS 55.0%
TTTGTGGGTCACTGCGAGTC+CGG + chr4_2:69385758-69385777 Msa0618410:CDS 55.0%
TGAGGAGTGAGTGAAGAGGC+AGG - chr4_2:69385060-69385079 None:intergenic 55.0%
TCTGGGAAGAGGCAGTGCAT+TGG + chr4_2:69385173-69385192 Msa0618410:CDS 55.0%
GCTGCTACCTACACTGTTGG+AGG + chr4_2:69385240-69385259 Msa0618410:CDS 55.0%
ACCTACACTGTTGGAGGTCC+TGG + chr4_2:69385246-69385265 Msa0618410:CDS 55.0%
TCCAGGACCTCCAACAGTGT+AGG - chr4_2:69385250-69385269 None:intergenic 55.0%
TACACTGTTGGAGGTCCTGG+AGG + chr4_2:69385249-69385268 Msa0618410:CDS 55.0%
GCCACCACATTGTGAGCTGA+TGG - chr4_2:69385626-69385645 None:intergenic 55.0%
GTGGTGGCAGTGAACAAAGG+AGG + chr4_2:69385638-69385657 Msa0618410:CDS 55.0%
TTTGTGGGTCACTGCGAGTC+CGG + chr4_2:69385758-69385777 Msa0618410:CDS 55.0%
TTGGGCAGACCCACGTGTCT+TGG + chr4_2:69384962-69384981 Msa0618410:five_prime_UTR 60.0%
CTGTTGGAGGTCCTGGAGGT+TGG + chr4_2:69385253-69385272 Msa0618410:CDS 60.0%
TTGGGCAGACCCACGTGTCT+TGG + chr4_2:69384962-69384981 Msa0618410:five_prime_UTR 60.0%
CTGTTGGAGGTCCTGGAGGT+TGG + chr4_2:69385253-69385272 Msa0618410:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 69384933 69386150 69384933 ID=Msa0618410;Name=Msa0618410
chr4_2 mRNA 69384933 69386150 69384933 ID=Msa0618410-mRNA-1;Parent=Msa0618410;Name=Msa0618410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=235|1|1|1|1|1|2|342|121
chr4_2 exon 69384933 69385333 69384933 ID=Msa0618410-mRNA-1:exon:20032;Parent=Msa0618410-mRNA-1
chr4_2 exon 69385609 69386150 69385609 ID=Msa0618410-mRNA-1:exon:20033;Parent=Msa0618410-mRNA-1
chr4_2 five_prime_UTR 69384933 69385167 69384933 ID=Msa0618410-mRNA-1:five_prime_utr;Parent=Msa0618410-mRNA-1
chr4_2 CDS 69385168 69385333 69385168 ID=Msa0618410-mRNA-1:cds;Parent=Msa0618410-mRNA-1
chr4_2 CDS 69385609 69385808 69385609 ID=Msa0618410-mRNA-1:cds;Parent=Msa0618410-mRNA-1
chr4_2 three_prime_UTR 69385809 69386150 69385809 ID=Msa0618410-mRNA-1:three_prime_utr;Parent=Msa0618410-mRNA-1
Gene Sequence

>Msa0618410

ATGGCTCTGGGAAGAGGCAGTGCATTGGTTCTACTAGTCTGCTTTTTTGTTCTTAACTCTGAGTTTGCTCATGCTGCTACCTACACTGTTGGAGGTCCTGGAGGTTGGACCTTTAACACTGTTGGTTGGCCTAAGGGAAAACGTTTTAGGGCTGGTGACACACTTGTGTTCAACTATAGTCCATCAGCTCACAATGTGGTGGCAGTGAACAAAGGAGGGTATGATAGTTGCAAGACACCAAGAGGAGCAAAAGTGTATAGGTCAGGAAAGGATCAGATAAGGCTTGCTAGGGGACAAAACTACTTCATTTGCAACTTTGTGGGTCACTGCGAGTCCGGAATGAAAATTGCCATCAATGCTGCTTAA
Protein sequence

>Msa0618410

MALGRGSALVLLVCFFVLNSEFAHAATYTVGGPGGWTFNTVGWPKGKRFRAGDTLVFNYSPSAHNVVAVNKGGYDSCKTPRGAKVYRSGKDQIRLARGQNYFICNFVGHCESGMKIAINAA*